CN114026240A - 靶向基因编辑构建体及其使用方法 - Google Patents

靶向基因编辑构建体及其使用方法 Download PDF

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CN114026240A
CN114026240A CN202080043025.3A CN202080043025A CN114026240A CN 114026240 A CN114026240 A CN 114026240A CN 202080043025 A CN202080043025 A CN 202080043025A CN 114026240 A CN114026240 A CN 114026240A
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nucleic acid
amino acid
sequence
modified
protein
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A·桑切斯-梅贾斯·加西亚
M·格尔·卡戈尔
D·伊万西克·德杰马诺维克
M·帕拉雷斯·马斯米加
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Universitat Pompeu Fabra UPF
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Universitat Pompeu Fabra UPF
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CN202080043025.3A 2019-06-11 2020-06-11 靶向基因编辑构建体及其使用方法 Pending CN114026240A (zh)

Applications Claiming Priority (3)

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US201962860186P 2019-06-11 2019-06-11
US62/860,186 2019-06-11
PCT/IB2020/055507 WO2020250181A1 (en) 2019-06-11 2020-06-11 Targeted gene editing constructs and methods of using the same

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US (1) US20220235379A1 (https=)
EP (1) EP3983541A1 (https=)
JP (1) JP7830129B2 (https=)
KR (1) KR20220019794A (https=)
CN (1) CN114026240A (https=)
AU (1) AU2020290790B2 (https=)
BR (1) BR112021024828A2 (https=)
CA (1) CA3141422A1 (https=)
IL (1) IL288794A (https=)
MX (1) MX2021015157A (https=)
WO (1) WO2020250181A1 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116218904A (zh) * 2022-11-03 2023-06-06 国药中生生物技术研究院有限公司 一种可提高工程化细胞外泌体中特定核酸分子载量的方法及其应用
CN118185901A (zh) * 2022-12-12 2024-06-14 上海精缮生物科技有限责任公司 一种PBase蛋白、融合蛋白、核酸和基因整合系统及应用
CN119053694A (zh) * 2023-03-27 2024-11-29 北京星辰集因科技有限责任公司 一种分离的转座酶及其用途

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JP7419168B2 (ja) * 2020-06-10 2024-01-22 株式会社東芝 改変型ｐｉｇｇｙＢａｃトランスポゼースのポリペプチド、それをコードするポリヌクレオチド、導入キャリア、キット、細胞のゲノムに目的配列を組込む方法及び細胞製造方法
AU2021403660A1 (en) 2020-12-16 2023-07-06 Universitat Pompeu Fabra Programmable transposases and uses thereof
JP2023553701A (ja) * 2020-12-16 2023-12-25 ウニベルシタット ポンペウ ファブラ 先天性筋ジストロフィーの処置のための治療用ｌａｍａ２ペイロード
EP4341392A4 (en) * 2021-05-14 2025-02-19 Becton Dickinson and Company Multiplexed unbiased nucleic acid amplification method
CA3234642A1 (en) * 2021-10-04 2023-04-13 Poseida Therapeutics, Inc. Transposases and uses thereof
JP2024545923A (ja) * 2021-12-30 2024-12-13 リーゲル セラピューティクス，インコーポレイティッド ナトリウム電位依存性チャネルアルファサブユニット１の発現を調節するための組成物、及びその使用
WO2023141504A2 (en) * 2022-01-19 2023-07-27 Genomeminer, Inc. (Formally Tupac Bio, Inc.) Dcas9-integrase for targeted genome editing
KR20230122932A (ko) 2022-02-15 2023-08-22 주식회사 엘지에너지솔루션 이차전지 제조장치 및 이를 이용하는 이차전지 제조방법
CN121712899A (zh) * 2023-04-27 2026-03-20 伦斯勒理工学院 用于在真核细胞中准确插入dna序列的重组酶
CN121443733A (zh) 2023-06-01 2026-01-30 英特格拉治疗公司 转座酶及其用途
WO2025212757A2 (en) * 2024-04-03 2025-10-09 Buck Institute For Research On Aging Cas enabled targeted transposition
CN119842638A (zh) * 2025-01-20 2025-04-18 天津大学 融合酶突变体及其在氟代白藜芦醇合成中的应用

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WO2016161207A1 (en) * 2015-03-31 2016-10-06 Exeligen Scientific, Inc. Cas 9 retroviral integrase and cas 9 recombinase systems for targeted incorporation of a dna sequence into a genome of a cell or organism
WO2018175872A1 (en) * 2017-03-24 2018-09-27 President And Fellows Of Harvard College Methods of genome engineering by nuclease-transposase fusion proteins

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DE60335637D1 (de) * 2002-07-24 2011-02-17 Manoa Biosciences Inc Vektoren auf transposonbasis und verfahren zur integration von nukleinsäuren
WO2013012824A2 (en) * 2011-07-15 2013-01-24 The Johns Hopkins University Trichoplusia ni piggybac transposases with reduced integration activity
US20170114333A1 (en) * 2014-04-11 2017-04-27 The Johns Hopkins University Improvements to eukaryotic transposase mutants and transposon end compositions for modifying nucleic acids and methods for production and use in the generation of sequencing libraries
KR102249982B1 (ko) * 2015-12-14 2021-05-11 게놈프론티어 테라퓨틱스 인코포레이티드 트랜스포존 시스템, 이를 포함한 키트, 및 이들의 용도

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
WO2016161207A1 (en) * 2015-03-31 2016-10-06 Exeligen Scientific, Inc. Cas 9 retroviral integrase and cas 9 recombinase systems for targeted incorporation of a dna sequence into a genome of a cell or organism
WO2018175872A1 (en) * 2017-03-24 2018-09-27 President And Fellows Of Harvard College Methods of genome engineering by nuclease-transposase fusion proteins

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116218904A (zh) * 2022-11-03 2023-06-06 国药中生生物技术研究院有限公司 一种可提高工程化细胞外泌体中特定核酸分子载量的方法及其应用
CN118185901A (zh) * 2022-12-12 2024-06-14 上海精缮生物科技有限责任公司 一种PBase蛋白、融合蛋白、核酸和基因整合系统及应用
CN119053694A (zh) * 2023-03-27 2024-11-29 北京星辰集因科技有限责任公司 一种分离的转座酶及其用途

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IL288794A (en) 2022-02-01
KR20220019794A (ko) 2022-02-17
AU2020290790A1 (en) 2022-01-27
WO2020250181A1 (en) 2020-12-17
EP3983541A1 (en) 2022-04-20
JP2022540318A (ja) 2022-09-15
US20220235379A1 (en) 2022-07-28
BR112021024828A2 (pt) 2022-01-25

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