MX2021015157A - Construcciones de edicion de genes dirigidas y metodos de uso de las mismas. - Google Patents

Construcciones de edicion de genes dirigidas y metodos de uso de las mismas.

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Publication number
MX2021015157A
MX2021015157A MX2021015157A MX2021015157A MX2021015157A MX 2021015157 A MX2021015157 A MX 2021015157A MX 2021015157 A MX2021015157 A MX 2021015157A MX 2021015157 A MX2021015157 A MX 2021015157A MX 2021015157 A MX2021015157 A MX 2021015157A
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MX
Mexico
Prior art keywords
nucleic acid
methods
same
gene editing
targeted gene
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Application number
MX2021015157A
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English (en)
Inventor
- MEJÍAS GARCÍA Avencia SÁNCHEZ
Cargol Marc Güell
Djermanovic Dimitrie Ivancic
Masmitja Maria Pallarès
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Univ Pompeu Fabra
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Application filed by Univ Pompeu Fabra filed Critical Univ Pompeu Fabra
Publication of MX2021015157A publication Critical patent/MX2021015157A/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
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    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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  • Health & Medical Sciences (AREA)
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  • Genetics & Genomics (AREA)
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  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Physics & Mathematics (AREA)
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  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
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  • Enzymes And Modification Thereof (AREA)

Abstract

La presente divulgación proporciona las construcciones de ácidos nucleicos para usarse en la mejora de la inserción específica del sitio de un ácido nucleico exógeno en un genoma. En algunos aspectos, la construcción del ácido nucleico comprende una primera secuencia de polinucleótidos que codifica una proteína de enlace al ADN que se diseña de una forma tal como para que se enlace a una secuencia de ADN genómico específica, un segundo polinucleótido que comprende una integrasa modificada o una transposasa modificada que permite que se haga la inserción del ácido nucleico exógeno en el genoma, y una secuencia de ácido nucleico que codifica un enlazador entre los dos nucleótidos. En algunas modalidades, la construcción de ácido nucleico codifica una proteína de fusión, por ejemplo, una proteína de fusión para su suministro a una célula por medio de una partícula lentiviral.
MX2021015157A 2019-06-11 2020-06-11 Construcciones de edicion de genes dirigidas y metodos de uso de las mismas. MX2021015157A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962860186P 2019-06-11 2019-06-11
PCT/IB2020/055507 WO2020250181A1 (en) 2019-06-11 2020-06-11 Targeted gene editing constructs and methods of using the same

Publications (1)

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MX2021015157A true MX2021015157A (es) 2022-03-17

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US (1) US20220235379A1 (es)
EP (1) EP3983541A1 (es)
JP (1) JP2022540318A (es)
KR (1) KR20220019794A (es)
CN (1) CN114026240A (es)
AU (1) AU2020290790A1 (es)
BR (1) BR112021024828A2 (es)
CA (1) CA3141422A1 (es)
IL (1) IL288794A (es)
MX (1) MX2021015157A (es)
WO (1) WO2020250181A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022129438A1 (en) 2020-12-16 2022-06-23 Universitat Pompeu Fabra Programmable transposases and uses thereof
EP4263818A1 (en) * 2020-12-16 2023-10-25 Universitat Pompeu Fabra Therapeutic lama2 payload for treatment of congenital muscular dystrophy
EP4341392A1 (en) * 2021-05-14 2024-03-27 Becton Dickinson and Company Multiplexed unbiased nucleic acid amplification method
KR20240099249A (ko) * 2021-10-04 2024-06-28 포세이다 테라퓨틱스, 인크. 트랜스포사제 및 이의 용도
IL313824A (en) * 2021-12-30 2024-08-01 Regel Therapeutics Inc Compounds for modulating expression of sodium channel alpha subunit 1 and uses thereof
WO2023141504A2 (en) * 2022-01-19 2023-07-27 Genomeminer, Inc. (Formally Tupac Bio, Inc.) Dcas9-integrase for targeted genome editing
CN118185901A (zh) * 2022-12-12 2024-06-14 上海精缮生物科技有限责任公司 一种PBase蛋白、融合蛋白、核酸和基因整合系统及应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004009792A2 (en) * 2002-07-24 2004-01-29 Vanderbilt University Transposon-based vectors and methods of nucleic acid integration
EP3277805A1 (en) * 2015-03-31 2018-02-07 Exeligen Scientific, Inc. Cas 9 retroviral integrase and cas 9 recombinase systems for targeted incorporation of a dna sequence into a genome of a cell or organism
WO2018175872A1 (en) * 2017-03-24 2018-09-27 President And Fellows Of Harvard College Methods of genome engineering by nuclease-transposase fusion proteins

Also Published As

Publication number Publication date
US20220235379A1 (en) 2022-07-28
CN114026240A (zh) 2022-02-08
IL288794A (en) 2022-02-01
JP2022540318A (ja) 2022-09-15
CA3141422A1 (en) 2020-12-17
KR20220019794A (ko) 2022-02-17
WO2020250181A1 (en) 2020-12-17
AU2020290790A1 (en) 2022-01-27
BR112021024828A2 (pt) 2022-01-25
EP3983541A1 (en) 2022-04-20

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