CN113973599A - Tilia miqueliana cutting propagation method - Google Patents
Tilia miqueliana cutting propagation method Download PDFInfo
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- CN113973599A CN113973599A CN202111390869.8A CN202111390869A CN113973599A CN 113973599 A CN113973599 A CN 113973599A CN 202111390869 A CN202111390869 A CN 202111390869A CN 113973599 A CN113973599 A CN 113973599A
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- 238000005520 cutting process Methods 0.000 title claims abstract description 107
- 238000000034 method Methods 0.000 title claims abstract description 31
- 241000328973 Tilia miqueliana Species 0.000 title claims description 26
- 238000005507 spraying Methods 0.000 claims description 43
- 238000011282 treatment Methods 0.000 claims description 27
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- 239000002689 soil Substances 0.000 claims description 23
- 235000016709 nutrition Nutrition 0.000 claims description 15
- 230000035764 nutrition Effects 0.000 claims description 15
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- 240000007591 Tilia tomentosa Species 0.000 claims description 9
- 235000010840 Tilia tomentosa Nutrition 0.000 claims description 9
- 238000007598 dipping method Methods 0.000 claims description 9
- 235000015097 nutrients Nutrition 0.000 claims description 9
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- 238000009423 ventilation Methods 0.000 claims description 9
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- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 8
- 239000006013 carbendazim Substances 0.000 claims description 8
- 239000005556 hormone Substances 0.000 claims description 8
- 229940088597 hormone Drugs 0.000 claims description 8
- 239000003814 drug Substances 0.000 claims description 7
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 6
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 5
- 241001465754 Metazoa Species 0.000 claims description 5
- 210000003608 fece Anatomy 0.000 claims description 5
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 5
- 239000011790 ferrous sulphate Substances 0.000 claims description 5
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 5
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 5
- 239000010871 livestock manure Substances 0.000 claims description 5
- 239000004576 sand Substances 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 239000004563 wettable powder Substances 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 4
- 102000018997 Growth Hormone Human genes 0.000 claims description 3
- 108010051696 Growth Hormone Proteins 0.000 claims description 3
- 241000793823 Tilia amurensis Species 0.000 claims description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 3
- 230000032683 aging Effects 0.000 claims description 3
- 230000000844 anti-bacterial effect Effects 0.000 claims description 3
- 239000003899 bactericide agent Substances 0.000 claims description 3
- 239000004202 carbamide Substances 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 3
- 239000002131 composite material Substances 0.000 claims description 3
- 230000000249 desinfective effect Effects 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 239000003337 fertilizer Substances 0.000 claims description 3
- 239000000122 growth hormone Substances 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 238000003973 irrigation Methods 0.000 claims description 3
- 230000002262 irrigation Effects 0.000 claims description 3
- 230000007774 longterm Effects 0.000 claims description 3
- 239000002420 orchard Substances 0.000 claims description 3
- 230000008635 plant growth Effects 0.000 claims description 3
- 230000002035 prolonged effect Effects 0.000 claims description 3
- 238000012414 sterilization procedure Methods 0.000 claims description 3
- 235000013619 trace mineral Nutrition 0.000 claims description 3
- 239000011573 trace mineral Substances 0.000 claims description 3
- 238000009333 weeding Methods 0.000 claims description 3
- 235000002168 Tilia europaea Nutrition 0.000 claims description 2
- 240000006909 Tilia x europaea Species 0.000 claims description 2
- 238000003825 pressing Methods 0.000 claims description 2
- 238000013138 pruning Methods 0.000 claims description 2
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- 230000000694 effects Effects 0.000 description 13
- 239000010451 perlite Substances 0.000 description 9
- 235000019362 perlite Nutrition 0.000 description 9
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 6
- 244000025254 Cannabis sativa Species 0.000 description 6
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- 229910052799 carbon Inorganic materials 0.000 description 4
- 229930192334 Auxin Natural products 0.000 description 3
- 239000002363 auxin Substances 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 230000035876 healing Effects 0.000 description 3
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000035699 permeability Effects 0.000 description 3
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- 238000009331 sowing Methods 0.000 description 3
- HQRHFUYMGCHHJS-UHFFFAOYSA-N 2-[[2-[(2-aminoacetyl)amino]acetyl]amino]-5-(diaminomethylideneamino)pentanoic acid Chemical compound NCC(=O)NCC(=O)NC(C(O)=O)CCCN=C(N)N HQRHFUYMGCHHJS-UHFFFAOYSA-N 0.000 description 2
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- 239000003415 peat Substances 0.000 description 2
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- 230000001568 sexual effect Effects 0.000 description 2
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- 239000005747 Chlorothalonil Substances 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 241000219071 Malvaceae Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
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- 244000141353 Prunus domestica Species 0.000 description 1
- 241000793824 Tilia henryana Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- CRQQGFGUEAVUIL-UHFFFAOYSA-N chlorothalonil Chemical compound ClC1=C(Cl)C(C#N)=C(Cl)C(C#N)=C1Cl CRQQGFGUEAVUIL-UHFFFAOYSA-N 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
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- 235000009566 rice Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
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- Life Sciences & Earth Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Developmental Biology & Embryology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to the technical field of tilia henryi breeding, in particular to a tilia henryi cutting propagation method, which is different from a conventional cutting collection method.
Description
Technical Field
The invention relates to the technical field of tilia henryi cultivation and propagation, in particular to a tilia henryi cutting propagation method.
Background
Tilia miqueliana (academic name:Tilia henryana) The Tilia miqueliana Miqueliana is a plant of Tilia of Tiliaceae, belongs to a plant variety special for China, is also an excellent native tree species, is mainly distributed in Shaanxi, Hunan, Jiangxi, Jiangsu, Hubei, Henan and the like in continental China, generally grows in mountains with the altitude of below 1400 meters, Tilia miqueliana Miqueliana likes yang, seedlings are slightly resistant to yin, drought and barren, slightly resistant to cold, and not strict in requirements on soil, the Tilia miqueliana Miqueliana is straight and tall in body, excellent in material quality, elegant in tree shape, dense in branches and leaves, long in service life, has been artificially introduced and cultivated at present, has strong adaptability and shows no error at all altitudes, however, when the Tilia miqueliana is bred in a sexual propagation mode, the seed peel is hard, the water permeability is poor, if not treated, after sowing, the germination rate is extremely low in the year, and generally needs two to three years or even longer to landSeedling emergence is continued, a sowing land is occupied for a long time, the operation cost is increased, and the seedling growing period is longer, so that the sowing and seedling growing method cannot well meet the requirement for rapid breeding of tilia tomentosa.
Disclosure of Invention
The invention aims to provide a tilia henryi cuttage propagation method, which can realize mass propagation and seedling raising of tilia henryi in a short period within a manual control range, can be transplanted to a field in the same year, can grow seedlings in the same year, and greatly meets the propagation requirements of people on tilia henryi.
In order to achieve the purpose, the invention adopts the following technical scheme:
a Tilia miqueliana cutting propagation method mainly comprises the following steps:
s1, constructing cutting orchard
S11, selecting an irrigation convenience soil layer deep nursery land, completing mechanical deep ploughing and soil preparation in winter in the previous year, applying decomposed and fermented animal manure per mu in combination with soil preparation, and simultaneously spreading ferrous sulfate;
s12, selecting a biennial excellent tilia europaea grafted seedling, planting the seedling with full buds in the late March without mechanical damage, watering for fixing roots after planting, and watering thoroughly to ensure that the root system is in close contact with soil;
s2, selection and treatment of cutting slips
S21, selection of cutting slips: in the late May and early June, when the sprouting length of the trunk of the cutting garden is 15-20 cm and the leaves are 5-7, selecting newly-germinated twigs on annual twigs of Tilia tomentosa fixedly planted in the cutting garden as cuttings;
s22, cutting the cutting shoot: collected twigs are made into cutting slips as soon as possible, the cutting slips are collected, made and inserted at any time, leaves of the soil entering section are removed, then larger leaves at the lower parts of the twigs are cut off from 1/3-1/2, the tops of the twigs are reserved, and then bases of the twigs are treated with medicaments in order;
s23, hormone treatment: treating with high-concentration plant growth hormone, sterilizing cutting slips with 400 times of 25% carbendazim wettable powder solution before treatment, and cutting after drying;
s3, seedling raising facility and substrate preparation
S31, adopting a sunlight greenhouse, installing an automatic spraying system in the greenhouse, arranging a seedling raising pond in the greenhouse with a sun-shading facility outside the greenhouse, paving a layer of coarse sand on the bottom layer of the seedling raising pond, raising seedlings by using a hole tray or a nutrition cup, filling the prepared nutrition medium into the seedling raising pond, and putting the seedling raising pond in order;
s4, cuttage and cultivation management
S41, cuttage: cutting the base part of the cutting slips after quick dipping treatment, wherein the depth is 2 cm-4 cm, the base part and the cutting slips are tightly combined and cannot reach the volt due to finger pressing, and the density is based on that leaves between the cutting slips are not overlapped;
s42, cultivation management measures: the method specifically comprises the following steps of (1) carrying out a moisture management program; sterilizing and disinfecting; a lighting management program; temperature and humidity control program; a nutrition management program; hardening and transplanting the seedlings; and (5) transplanting and managing after planting.
Further, in the step S11, 1000 kg of decomposed and fermented animal manure is applied per mu, 20 kg of ferrous sulfate is simultaneously applied per mu, and the deep ploughing depth is 25 cm-30 cm.
Further, in step S21, when cutting and picking, it is necessary for the operator to hold the twig with one hand and cut off the twig at the base of the branch close to the old branch with a pruning scissors with the other hand, and the picked twig is brought back to make the cutting.
Further, in the step S23, a high-concentration auxin quick dipping treatment is adopted, before the treatment, 400 times of solution of 25% carbendazim wettable powder is used for disinfecting the cutting slips, the Tilia tomentosa twig cutting slips are quickly dipped for 3-5S through the auxin, and the cutting can be carried out after slight drying.
Further, in step S42, the cultivation management measures specifically include: and (3) a water management program: the spraying system controlled by the automatic spraying seedling raising instrument is used for adjusting related data according to weather conditions, so that the plant leaves are dry, wet and damp, and do not wither or rot due to long-term excessive moisture; the spraying standard of the non-rooted cutting is as follows: the water spraying time and the water spraying amount are adjusted at any time according to the change of weather and temperature. When the moisture of the leaves is gradually volatilized after the last spraying, and the next spraying is started when the 2/3 leaves are just dried and 1/3 leaves still have moisture; after the cutting shoots root, the interval time of the seedling raising instrument needs to be adjusted in time, the time of spraying intervals is relatively gradually prolonged, and the water spraying amount is reduced;
and (3) disinfection and sterilization procedures: in the process of seedling culture, broad-spectrum bactericide is required to be sprayed every 5-7 days;
the illumination management program: the method is characterized in that proper shading is needed in the early seedling stage, a shading net is used, excessive shading cannot be achieved, after the rooting starts for 10-15 days, the shading net is gradually removed, and the following operation can be specifically adopted: shading when sunlight is strong at noon, and removing the shading net before 10 am and after 17 pm every day until ventilation hardening seedlings are started without shading;
temperature and humidity control program: the operator needs to keep the temperature in the shed between 18 ℃ and 35 ℃ and the relative humidity between 80 percent and 95 percent all the time;
and (4) nutrition management program: spraying low-concentration nutrient solution every 5-7 days, specifically, spraying 0.1% of monopotassium phosphate before rooting, and spraying 0.2% of nutrient solution every 3-5 days after rooting, wherein the nutrient solution can be 50% of urea, 40% of monopotassium phosphate or 10% of composite trace elements;
hardening and transplanting programs: tilia tomentosa is rooted after 10-15 days of cuttage, the rooting can reach more than 90% after 20-25 days of rooting, ventilation and seedling hardening can be gradually started, the water spraying amount is gradually reduced, the rooting seedling aging is promoted, and field transplanting can be carried out after 7-15 days of exercise;
transplanting and post-planting management program: when the exercised tilia amurensis root seedlings are transplanted, the hole trays or the nutrition cups need to be removed, soil balls need to be kept complete, holes are dug in the prepared field for planting, watering is carried out in time after planting, watering is carried out for the second time within 3 d-5 d, loosening the soil and weeding are carried out after the ground skin is dried, water and fertilizer management is enhanced after the seedlings survive and grow, and the seedlings can grow in the same year.
The invention has the beneficial effects that: the invention provides a Tilia miqueliana cutting propagation method, which is different from a conventional cutting collection method, and comprises the steps of firstly building a cutting garden, carrying out drug treatment on cutting slips and a matrix before cutting, and carrying out environmental control on the Tilia miqueliana after cutting.
Drawings
FIG. 1 is a table comparing the effect of different hormone treatments on the rooting behavior of cuttings;
FIG. 2 is a table comparing the effect of different concentrations of IBA treatment on cutting rooting and healing;
FIG. 3 is a table showing the comparison of root-growing effect of Tilia tomentose by cutting in different substrate states;
FIG. 4 is a photograph showing the operation of sterilizing the ear of the rice in example 1;
FIG. 5 is a photograph showing the rooting of callus in example 1;
FIG. 6 is a photograph showing the rooting of Tilia tomentose in example 1;
FIG. 7 is a photograph I of the growth of the stem segment in example 1;
FIG. 8 is a photograph II of the growth of the stem section in example 1;
FIG. 9 is photograph I of the root growth in example 1;
FIG. 10 is photograph II of the root growth in example 1;
FIG. 11 is a photograph showing the root growth of the nursery in example 1;
FIG. 12 is a photograph showing the growth of nursery cuttings according to example 1.
Detailed Description
Specific example 1: the technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. It should be noted that: in the present invention, all the embodiments and preferred methods mentioned herein can be combined with each other to form a new technical solution, if not specifically stated. In the present invention, all the technical features mentioned herein and preferred features may be combined with each other to form a new technical solution, if not specifically stated. In the present invention, the components referred to or the preferred components thereof may be combined with each other to form a novel embodiment, if not specifically stated. In the present invention, unless otherwise specified, the individual reactions or operation steps may be performed sequentially or may be performed in sequence. Preferably, the reaction processes herein are carried out sequentially. Unless otherwise defined, technical and scientific terms used herein have the same meaning as is familiar to those skilled in the art. In addition, any methods or materials similar or equivalent to those described herein can also be used in the present invention. In order to solve the technical problems of low germination rate, long seedling culture period and high seedling culture cost of the current year when tilia henryi is propagated and cultured in a sexual propagation mode, the invention provides a tilia henryi cutting propagation method, which can enable the cuttage survival rate of the tilia henryi to reach 90 percent and the transplanting survival rate to reach 95 percent, and the specific operation comprises the following steps:
s1, constructing cutting orchard
S1.1: selecting nursery lands convenient for irrigation and having deep soil layers, completing mechanical deep ploughing and soil preparation in winter in the previous year, combining soil preparation, applying 1000 kg of decomposed and fermented animal manure per mu, simultaneously applying 20 kg of ferrous sulfate per mu, and deeply ploughing to a depth of 25-30 cm;
s1.2: selecting a seedling grafted by the biennial excellent-hair Tilia miqueliana, wherein the average seedling height is 120-150 cm, the main root length is more than 20cm, the lateral roots are more than 3, the ground diameter is more than 2.5 cm, the seedling is straight, no mechanical damage is ensured by checking, the seedling with full buds is fixedly planted in the third ten days of the next year, the row spacing is 1 m multiplied by 1.5 m, after planting, root water is poured, and the root system is thoroughly poured to ensure that the soil is in close contact with the root system;
s2, selection and treatment of cutting slips
S21, collection of cutting shoots: in the late May or early June of the current year, when the sprouting length of the trunk of the cutting garden is 15-20 cm and the number of leaves is 5-7, cutting shoots are collected in the morning of cloudy days or sunny days, and specifically, newly-germinated twigs on 1-year-old branches of the Tilia tomentosa fixedly planted in the cutting garden can be selected as the cutting shoots, and the specific collection method comprises the following steps: an operator holds the tender branches with one hand, and prunes the tender branches at the positions, close to the old branches, of the base parts of the tender branches with the other hand, and the collected tender branches are brought back to be made into cutting slips;
s22, cutting the cutting shoot: collected twigs are made into cutting slips as soon as possible, the cutting slips are collected, made and inserted at any time, leaves of the soil entering section are removed, then larger leaves at the lower parts of the twigs are cut off from 1/3-1/2, the tops of the twigs are reserved, and then bases of the twigs are treated with medicaments in order;
s23, hormone treatment: adopting plant growth hormone with higher concentration to carry out quick dipping treatment, using 400 times of solution of 25% carbendazim wettable powder to disinfect the cutting slips before treatment, obtaining the optimum concentration of IBA 2.0 mg/ml for cutting slips treatment of young twigs of tilia miqueliana through experimental study, carrying out quick dipping for 3-5 s, and carrying out cutting after slight airing;
s3, seedling raising facility and substrate preparation
S31, adopting a sunlight greenhouse as a seedling growing facility, installing an automatic spraying system in the greenhouse, arranging a sun shading facility outside the greenhouse, arranging a seedling growing pond in the greenhouse, paving a layer of coarse sand on the bottom layer of the seedling growing pond, growing seedlings by using a hole tray or a nutrition cup, filling prepared nutrition medium into the seedling growing pond, and neatly placing the seedling growing pond. Uniformly mixing the cutting matrix with turf and perlite according to a ratio of 2:1, spraying 0.5% potassium permanganate solution or 0.1% carbendazim for disinfection before cutting, and stacking for 24 h for use;
s4, cuttage and cultivation management
S41, cuttage: cutting after the base part of the cutting slips is subjected to quick dipping treatment, the depth is 2 cm-4 cm, the combination of the matrix and the cutting slips is tight and less than the volt when the finger is pressed, the density is that the leaves between the cutting slips are not overlapped, and after the cutting is finished, the cultivation management is developed according to further management measures;
s42, cultivation management measures: and (3) a water management program: the spraying system controlled by the automatic spraying seedling raising instrument is used for adjusting related data according to weather conditions, so that plant leaves are dry, wet and dry, and do not wither or rot due to long-term excessive moisture. The spraying standard of the non-rooted cutting is as follows: the water spraying time and the water spraying amount are adjusted at any time according to the change of weather and temperature. When the moisture of the leaves is gradually volatilized after the last spraying, and the water is still remained in the 1/3 leaves just dried at 2/3, the next spraying is started. After the cutting shoots root, the interval time of the seedling raising instrument needs to be adjusted in time, the time of spraying intervals is relatively gradually prolonged, and the water spraying amount is reduced;
and (3) disinfection and sterilization procedures: mainly means that in the process of seedling culture, 800 times of broad-spectrum bactericides such as carbendazim, chlorothalonil, procymidone and the like are required to be sprayed every 5 d-7 d, and the medicaments can be used alternately; the illumination management program: proper shading is needed in the early stage of seedling culture, a shading net with shading rate of about 30% is used, excessive shading is avoided, after the rooting starts for 10 d-15 d, the shading net needs to be removed gradually, and the specific operation process is as follows: shading when sunlight is strong at noon, the shading net needs to be removed before 10 am and after 17 pm every day until ventilation hardening seedlings are started without shading;
temperature and humidity control program: the temperature in the greenhouse is required to be kept between 18 ℃ and 35 ℃ all the time, and the relative humidity is 80 percent to 95 percent;
and (4) nutrition management program: spraying low-concentration nutrient solution every 5-7 days, spraying 0.1% of monopotassium phosphate before rooting, and spraying 0.2% of nutrient solution every 3-5 days after rooting, wherein the nutrient solution comprises 50% of urea, 40% of monopotassium phosphate and 10% of composite trace elements;
hardening and transplanting programs: tilia tomentosa starts to root after 10-15 days of cuttage, the rooting can reach more than 90% after 20-25 days, ventilation and seedling hardening can be gradually started in the process, the water spraying amount is gradually reduced, the aging of the rooted seedlings is promoted, and the field transplantation can be carried out after 7-15 days of exercise;
transplanting and post-planting management program: when the exercised tilia amurensis root seedlings are transplanted, the hole trays or the nutrition cups need to be removed, soil balls need to be kept complete, holes are dug in the prepared field for planting, watering is carried out in time after planting, watering is carried out for the second time within 3 d-5 d, loosening the soil and weeding are carried out after the ground skin is dried, water and fertilizer management is enhanced after the seedlings survive and grow, and the seedlings can grow in the same year.
The cutting propagation method of tilia miqueliana of the invention is verified by the following tests:
firstly, verifying the influence of different hormone treatments on the rooting of the cutting shoot
By adopting the cutting propagation cultivation method, different hormones are adopted to treat the cutting shoots by 2mg/ml, and the result is shown in table 1 in the attached figure 1 of the specification: as can be seen from table 1 in figure 1 of the specification: the rooting rate is 92.5% of IBA (indolebutyric acid), 88.6% of IBA (indolebutyric acid) + NAA (naphthylacetic acid), 64.3% of NAA (naphthylacetic acid), 85.7% of GGR (rooting powder) and 8.2% of clear water, the average root number and the average root length of each treated rooted seedling are greatly improved compared with those of a control, and the root number is also much higher than that of the control, wherein the IBA treatment rooting rate is highest, the average root number is the most, the average root length is the largest, and the cuttage effect is the best;
secondly, verifying the influence of different concentration treatments of the same hormone on the rooting of the cutting shoot
The Tilia miqueliana branch cuttage needs the induction of exogenous auxin, and the test result shows that the effect of IBA is obviously superior to that of NAA, the rooting rate is high and stable, so that IBA is selected as the exogenous hormone for inducing rooting, the IBA treatment concentration is researched, and the result is shown in the table 2 in the attached figure 2 of the specification;
as can be seen from Table 2 in FIG. 2 of the specification: IBA concentration of 1 mg/ml-2 mg/ml can induce higher rooting effect, the rooting rate can reach 88% -92.6%, most incisions can be healed, and the healing rate is 90.5% -94.2%. When the IBA concentration is low, the incision can be healed, but the rooting rate is low, the rooting time is long, and the number of root systems is small; the healing rate and rooting rate of the incision are obviously reduced due to the over-high concentration of IBA; in the test, the IBA concentration is higher, the medicine dipping part of the cutting is blackened, the root system can be healed and grown above the medicine dipping part, and partial cutting blades fall off and die;
thirdly, verifying the relation between different matrixes and the cutting rooting effect
The test adopts 5 matrixes of turf 2 parts and perlite 1 part, turf, perlite, river sand and raw yellow soil, adopts IBA 2mg/ml to treat the cutting, and the result is shown in Table 3 in the attached figure 3 of the specification;
as seen from the table 3 in the attached figure 3 of the specification, 5 kinds of substrates have the best rooting effect by grass carbon, perlite and two kinds of mixed materials, the river sand effect is also good, the immature loess is used as the substrate, the cuttage rooting rate is the lowest, the reason that the rooting effect of the first 4 kinds of substrates is good has a direct relation with the good ventilation and water permeability of the substrates, and the better mixing effect of the grass carbon and the perlite is because the grass carbon and the perlite are rich in nutrition and the substrates have higher temperature and good ventilation property; the raw loess has poor drainage and ventilation, so the rooting rate is poor, the matrix with good permeability has good effect in terms of root number, and the mixed matrix is longer in terms of root length, so the grass carbon and the perlite are mixed and are more suitable to be used as the matrix for the twig cuttage of Tilia tomentosa;
fourth, conclusion
From the verification process, the Tilia miqueliana Maxim cutting slips are not resistant to water moisture, the cutting slips are easy to rot and die due to the fact that the water content of the substrate is too high, the cut shoots can be easily rotten and die, the cut twigs can be treated by grass peat and perlite as the substrates, the method is economical and practical, the rooting seedlings grow well, the seedling raising and transplanting are convenient, NAA, IBA and GGR treatment can promote the Tilia miqueliana Maxim twigs to heal and take root in advance, IBA treatment can obviously improve the rooting rate of the cutting slips and the quality of the rooting seedlings, wherein grass peat and perlite are uniformly mixed according to the ratio of 2:1, a 0.5% potassium permanganate solution or 0.1% carbendazim is used for disinfection before cutting, on the basis of the cutting substrates used after 24 hours are stacked, the Tilia miqueliana is used for treating the Tilia miqueliana under the concentration of 2mg/ml, the mortality is only 1.8%, the average number of the rooting seedlings of the Tilia miqueliana Tilia miqueliana, the average number of the cutting roots is more than 4, the average number of the root length is more than 7.0, and the best effect is achieved, can meet the requirements of seedling production. The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (5)
1. A Tilia miqueliana cutting propagation method is characterized by mainly comprising the following steps:
s1, constructing cutting orchard
S11, selecting an irrigation convenience soil layer deep nursery land, completing mechanical deep ploughing and soil preparation in winter in the previous year, applying decomposed and fermented animal manure per mu in combination with soil preparation, and simultaneously spreading ferrous sulfate;
s12, selecting a biennial excellent tilia europaea grafted seedling, planting the seedling with full buds in the late March without mechanical damage, watering for fixing roots after planting, and watering thoroughly to ensure that the root system is in close contact with soil;
s2, selection and treatment of cutting slips
S21, selection of cutting slips: in the late May and early June, when the sprouting length of the trunk of the cutting garden is 15-20 cm and the leaves are 5-7, selecting newly-germinated twigs on annual twigs of Tilia tomentosa fixedly planted in the cutting garden as cuttings;
s22, cutting the cutting shoot: collected twigs are made into cutting slips as soon as possible, the cutting slips are collected, made and inserted at any time, leaves of the soil entering section are firstly removed, then larger leaves at the lower parts of the twigs are cut off 1/3-1/2, the tops of the twigs are reserved, and then the bases of the twigs are treated with medicaments;
s23, hormone treatment: treating with high-concentration plant growth hormone, sterilizing cutting slips with 400 times of 25% carbendazim wettable powder solution before treatment, and cutting after drying;
s3, seedling raising facility and substrate preparation
S31, adopting a sunlight greenhouse, installing an automatic spraying system in the greenhouse, arranging a seedling raising pond in the greenhouse with a sun-shading facility outside the greenhouse, paving a layer of coarse sand on the bottom layer of the seedling raising pond, raising seedlings by using a hole tray or a nutrition cup, filling the prepared nutrition medium into the seedling raising pond, and putting the seedling raising pond in order;
s4, cuttage and cultivation management
S41, cuttage: cutting the base part of the cutting slips after quick dipping treatment, wherein the depth is 2 cm-4 cm, the base part and the cutting slips are tightly combined and cannot reach the volt due to finger pressing, and the density is based on that leaves between the cutting slips are not overlapped;
s42, cultivation management measures: the method specifically comprises the following steps of (1) carrying out a moisture management program; sterilizing and disinfecting; a lighting management program; temperature and humidity control program; a nutrition management program; hardening and transplanting the seedlings; and (5) transplanting and managing after planting.
2. The cutting propagation method of tilia miqueliana as claimed in claim 1, further comprising applying 1000 kg of decomposed and fermented animal manure per mu in step S11, and simultaneously applying 20 kg of ferrous sulfate per mu, and deep ploughing depth is 25 cm-30 cm.
3. The cutting propagation method of tilia miqueliana as claimed in claim 1, further comprising the step of S21, wherein when picking up the cutting shoot, the operator holds the twig with one hand and cuts off the twig at the base of the shoot close to the old shoot with the other hand by pruning shears, and the picked twig is brought back to make the cutting shoot.
4. The cutting propagation method of tilia miqueliana as claimed in claim 1, further comprising the step of S23, wherein the tilia miqueliana is treated by fast dipping with phytohormone with a high concentration, the step of disinfection treatment is carried out on the cutting slips by 400 times of 25% carbendazim wettable powder before treatment, the cutting slips of the tilia miqueliana twigs are dipped by the phytohormone for 3S to 5S, and the cutting can be carried out after slight drying.
5. The cutting propagation method of tilia miqueliana according to claim 1, further comprising, in step S42, the cultivation management measures specifically including: and (3) a water management program: the spraying system controlled by the automatic spraying seedling raising instrument is used for adjusting related data according to weather conditions, so that the plant leaves are dry, wet and damp, and do not wither or rot due to long-term excessive moisture; the spraying standard of the non-rooted cutting is as follows: adjusting the water spraying time and the water spraying amount at any time according to the change of weather and temperature; when the moisture of the leaves is gradually volatilized after the last spraying, and the next spraying is started when the 2/3 leaves are just dried and 1/3 leaves still have moisture; after the cutting shoots root, the interval time of the seedling raising instrument needs to be adjusted in time, the time of spraying intervals is relatively gradually prolonged, and the water spraying amount is reduced;
and (3) disinfection and sterilization procedures: in the process of seedling culture, broad-spectrum bactericide is required to be sprayed every 5-7 days;
the illumination management program: the method is characterized in that proper shading is needed in the early seedling stage, a shading net is used, excessive shading cannot be achieved, after the rooting starts for 10-15 days, the shading net is gradually removed, and the following operation can be specifically adopted: shading when sunlight is strong at noon, and removing the shading net before 10 am and after 17 pm every day until ventilation hardening seedlings are started without shading;
temperature and humidity control program: the operator needs to keep the temperature in the shed between 18 ℃ and 35 ℃ and the relative humidity between 80 percent and 95 percent all the time;
and (4) nutrition management program: spraying low-concentration nutrient solution every 5-7 days, specifically, spraying 0.1% of monopotassium phosphate before rooting, and spraying 0.2% of nutrient solution every 3-5 days after rooting, wherein the nutrient solution can be 50% of urea, 40% of monopotassium phosphate or 10% of composite trace elements;
hardening and transplanting programs: tilia tomentosa is rooted after 10-15 days of cuttage, the rooting can reach more than 90% after 20-25 days of rooting, ventilation and seedling hardening can be gradually started, the water spraying amount is gradually reduced, the rooting seedling aging is promoted, and field transplanting can be carried out after 7-15 days of exercise;
transplanting and post-planting management program: when the exercised tilia amurensis root seedlings are transplanted, the hole trays or the nutrition cups need to be removed, soil balls need to be kept complete, holes are dug in the prepared field for planting, watering is carried out in time after planting, watering is carried out for the second time within 3 d-5 d, loosening the soil and weeding are carried out after the ground skin is dried, water and fertilizer management is enhanced after the seedlings survive and grow, and the seedlings can grow in the same year.
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