CN113940246A - Potato original stock breeding matching technology - Google Patents
Potato original stock breeding matching technology Download PDFInfo
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- CN113940246A CN113940246A CN202111229895.2A CN202111229895A CN113940246A CN 113940246 A CN113940246 A CN 113940246A CN 202111229895 A CN202111229895 A CN 202111229895A CN 113940246 A CN113940246 A CN 113940246A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/17—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing slag
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Abstract
The invention belongs to the technical field of potatoes and discloses a potato breeder mating technology, S1, removing viruses in plants by stem tip stripping and heat treatment, then cutting off the top of the growing bud with a knife and placing the cut top into a culture medium for cultivation. The invention can solve the problem of too much or too little watering by combining the coal cinder and the sterile cloth, water is poured on the surface of the nutrient soil layer, the soft soil layer can not retain the water, the water can permeate the nutrient soil layer and flows into the coal cinder under the filtration of the sterile cloth, the phenomenon of dead seedling can be avoided because the fragile root of the test-tube seedling is gradually rotted in the moist nutrient soil with water for a long time, the survival rate of the test-tube seedling is influenced, the sterile cloth is arranged, the upper-layer nutrient soil can be prevented from losing, and most of nutrient components are prevented from losing; because the temperature in the greenhouse is generally higher, the water evaporation is faster, and the nutrient soil layer is easy to dry, the potato breeder stock is better.
Description
Technical Field
The invention belongs to the technical field of potatoes, and particularly relates to a potato stock breeding matching technology.
Background
The potato tuber is edible, and is one of the important grain crops all over the world, the potato breeder seed is the process of harvesting the stock seed after the stock seed is planted, and is also the basis of virus-free fine variety breeding, the stock seed is bred by a fine variety breeding farm or a variety breeding unit through a stock seed production procedure, the purity is higher, the quality is better, the fine variety is a variety improved on the basis of the stock seed, the quality and the yield are better, the stock seed is the seed potato which is originally produced by using virus-free tube seedling transplantation or cuttage, and the most critical step of the potato breed regarding the yield and the quality is the breeding of the potato stock seed.
The traditional potato breeder's original seed breeding technology generally adopts soil cultivation, although the cultivation cost is reduced, the usability is wide, but the pure soil cultivation can not provide sufficient nutrition for the seedling, the phenomenon of dead seedling can appear in the growth process of some transplanted seedlings, thereby the survival rate of the test tube seedling is reduced, soil is loose and not breathable enough to cultivate soil, the growth of the seedling is influenced, the conditions such as illumination intensity and temperature can not be controlled, the growth environment of each stage of phase adaptation can not be provided for the test tube seedling, in addition, the proper amount of water is needed in the test tube seedling growth process, the watering amount is different in each stage, the watering amount can not be controlled by the existing watering mode, the growth speed of the test tube seedling is influenced easily too little, and the dead root phenomenon is easily caused too much.
Disclosure of Invention
Aiming at the problems, the invention provides a potato breeder stock breeding matching technology which has the advantage of good breeding effect.
In order to achieve the purpose, the invention provides the following technical scheme: a potato breeder stock breeding matching technology comprises the following steps:
s1, removing viruses in the plants by a stem tip stripping and heat treatment method, then cutting off the top points of the growing buds by a knife and putting the cut growing buds into a culture medium for cultivation;
s2, recording the date on the bottle for cultivating the growing bud and moving the bottle to a growing room of the tissue culture seedling for cultivating in an aseptic environment;
s3, paving a layer of coal cinder with the thickness of about 4-6 cm on the ground of the insect-proof sterile greenhouse, sterilizing, killing insects, applying fertilizer, paving a layer of sterile cloth on the surface of the coal cinder, and paving a layer of nutrient soil on the top of the coal cinder;
s4, selecting a strong and pollution-free test-tube plantlet with a plant height of 6-7 cm and more than 3 leaves, then hardening the test-tube plantlet in a natural air temperature environment for 3-6 days, taking out the test-tube plantlet from a bottle, and washing off a culture medium on roots of the test-tube plantlet with water;
s5, taking out the washed test-tube plantlets, transplanting the test-tube plantlets into prepared nutrient soil, keeping the plant-row spacing at 4-8 cm, enabling tips of the plantlets to leak out of the ground by 2-3 cm and exposing 2 leaves, then watering and laminating, and controlling the temperature to be 22-25 ℃ and the humidity to be 95%;
s6, after 7-8 days, regulating and controlling the indoor temperature to be 25-28 ℃ and the humidity to be 80%, fertilizing by using nutrient solution in the early stage, fertilizing by using fertilizers such as urea, monopotassium phosphate and diammonium phosphate after 30 days, and performing pest control once every week;
s7, when the plants grow to 80-120 days, 85% of the potato blocks grow to be more than 2.5g and become yellow, the potato blocks can be harvested, the harvested potato blocks are aired for a plurality of days until the potato skins are aged, then the potato blocks are bagged, labeled and moved to a storage warehouse, the temperature in the storage warehouse is kept at 2-4 ℃, and the humidity is 85%.
The growth buds after detoxification can be ensured not to carry other germs, and can grow steadily during tissue culture growth; the coal cinder, the sterile cloth and the nutrient soil are matched for use, so that the soil is loose, the air permeability is good, the root of the test-tube plantlet is prevented from being rotted in accumulated water, the required water can be provided for the nutrient layer in the absence of water, the effect of isolating water supply is achieved, and the survival rate of the test-tube plantlet is improved; selecting strong and pollution-free test-tube plantlets to ensure high survival rate, and infecting other test-tube plantlets without carrying diseased plants; the test-tube plantlets are transplanted after hardening, so that the adaptability of the test-tube plantlets can be enhanced, the weak stage is maintained by matching with nutrient solution in the early stage, and sufficient nutrients are provided for the plantlets through fertilization in the later stage; the outside of the rice is kept dry by airing during harvesting, the rice is not easy to rot, and the rice is packaged and labeled for storage, so that the variety and specification can be conveniently searched
According to a preferable technical scheme of the invention, the knives used in the S1 are sterilized and are sterilized once every time the knives are cut, so that the condition that other types of potatoes carry diseased plants and are infected to other potatoes through contact can be avoided, and the potato breeder seeds are healthy and safe.
According to a preferable technical scheme of the invention, the air vent valve is arranged at the bottle mouth for cultivating the growing buds in the S2, the temperature of the growing room of the tissue culture seedling is kept at 20-24 ℃ and the illumination intensity is 2600-3000 lx, the air vent valve can ensure that the oxygen amount in the culture bottle is sufficient and can well prevent pollutants from entering, so that the internal tissue culture seedling can grow healthily, and the temperature and the required illumination intensity are necessary factors which cannot be lost in the growth of the tissue culture seedling.
As a preferable technical scheme of the invention, the fertilizer in the S3 contains diammonium phosphate, chicken manure and potassium sulfate and phoxim particles, so that the fertilizer can provide certain nutrients and can prevent and control soil insects, and after watering, the nutrients can be better distributed, and a tamping foundation is laid, so that the nutrients are sufficient.
As a preferred technical scheme of the invention, in S5, before transplanting, the test-tube plantlets are picked up by sterile tweezers and washed, and then soaked in a naphthylacetic acid solution for 3-5S, and then planted, wherein the picked-up test-tube plantlets are not contaminated by germs by the sterile tweezers, and then soaked by the naphthylacetic acid after filtration and sterilization, so that cell division and expansion can be promoted, and the regulator is a plant growth regulator.
As a preferable technical scheme, the membrane can be uncovered after 4-7 days of transplanting in S5, and because the test-tube seedlings need to be watered after transplanting, the action of covering the membrane can preserve heat and moisture, a comfortable and stable growth environment is provided for the seedlings which are just transplanted, and the seedlings can stably live through the fragile early stage.
As a preferable technical scheme of the invention, the nutrient solution mentioned in S6 is an MS nutrient solution, which contains a large amount of nitrate, potassium and ammonium, and in addition, the inorganic salt and ion have higher concentration, so that the nutrient solution is a stable balanced solution, and can meet the nutritional and physiological needs of plant cells.
In a preferred technical scheme of the invention, in the step S7, the supply of water and fertilizer is stopped and the stems and leaves on the ground are removed in the previous week of harvesting, so that the soil can be ensured to be gradually dried, the skins of the potato blocks can be promoted to be gradually hardened, the picking work is convenient, the surfaces of the potato blocks are not easy to peel in the process, the interiors of the potato blocks are not polluted, and the viruses on the stems and leaves can be prevented from being transferred to the tubers.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention can solve the problem of too much or too little watering by combining the coal cinder and the sterile cloth, water is poured on the surface of the nutrient soil layer, the soft soil layer can not retain the water, the water can permeate the nutrient soil layer and flows into the coal cinder under the filtration of the sterile cloth, the phenomenon of dead seedling can be avoided because the fragile root of the test-tube seedling is gradually rotted in the moist nutrient soil with water for a long time, the survival rate of the test-tube seedling is influenced, the sterile cloth is arranged, the upper-layer nutrient soil can be prevented from losing, and most of nutrient components are prevented from losing; because the temperature in the greenhouse is generally higher, the water is evaporated faster, the nutrient soil layer is easy to dry, and the water accumulated in the coal slag can provide the required water for the test-tube plantlet through the bottom layer of the nutrient soil in the form of water vapor through the sterile cloth, so that the effect of isolating water supply is achieved, and the test-tube plantlet is prevented from dying roots; the temperature and the humidity in the temperature control chamber are adjusted, so that the test tube plantlets can grow healthily and strongly in each stage; by using the nutrient solution and the chemical fertilizer in a matching way, the adaptability of the test-tube plantlet can be enhanced, and the test-tube plantlet can safely pass the fragile early stage; thereby ensuring that the breeding of the potato breeder stock is better.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a potato breeder stock breeding matching technology, which comprises the following steps:
s1, removing viruses in the plants by a stem tip stripping and heat treatment method, then cutting off the top points of the growing buds by a knife and putting the cut growing buds into a culture medium for cultivation;
s2, recording the date on the bottle for cultivating the growing bud and moving the bottle to a growing room of the tissue culture seedling for cultivating in an aseptic environment;
s3, paving a layer of coal cinder with the thickness of about 4-6 cm on the ground of the insect-proof sterile greenhouse, sterilizing, killing insects, applying fertilizer, paving a layer of sterile cloth on the surface of the coal cinder, and paving a layer of nutrient soil on the top of the coal cinder;
s4, selecting a strong and pollution-free test-tube plantlet with a plant height of 6-7 cm and more than 3 leaves, then hardening the test-tube plantlet in a natural air temperature environment for 3-6 days, taking out the test-tube plantlet from a bottle, and washing off a culture medium on roots of the test-tube plantlet with water;
s5, taking out the washed test-tube plantlets, transplanting the test-tube plantlets into prepared nutrient soil, keeping the plant-row spacing at 4-8 cm, enabling tips of the plantlets to leak out of the ground by 2-3 cm and exposing 2 leaves, then watering and laminating, and controlling the temperature to be 22-25 ℃ and the humidity to be 95%;
s6, after 7-8 days, regulating and controlling the indoor temperature to be 25-28 ℃ and the humidity to be 80%, fertilizing by using nutrient solution in the early stage, fertilizing by using fertilizers such as urea, monopotassium phosphate and diammonium phosphate after 30 days, and performing pest control once every week;
s7, when the plants grow to 80-120 days, 85% of the potato blocks grow to be more than 2.5g and become yellow, the potato blocks can be harvested, the harvested potato blocks are aired for a plurality of days until the potato skins are aged, then the potato blocks are bagged, labeled and moved to a storage warehouse, the temperature in the storage warehouse is kept at 2-4 ℃, and the humidity is 85%.
The growth buds after detoxification can be ensured not to carry other germs, and can grow steadily during tissue culture growth; the coal cinder, the sterile cloth and the nutrient soil are matched for use, so that the soil is loose, the air permeability is good, the root of the test-tube plantlet is prevented from being rotted in accumulated water, the required water can be provided for the nutrient layer in the absence of water, the effect of isolating water supply is achieved, and the survival rate of the test-tube plantlet is improved; selecting strong and pollution-free test-tube plantlets to ensure high survival rate, and infecting other test-tube plantlets without carrying diseased plants; the test-tube plantlets are transplanted after hardening, so that the adaptability of the test-tube plantlets can be enhanced, the weak stage is maintained by matching with nutrient solution in the early stage, and sufficient nutrients are provided for the plantlets through fertilization in the later stage; the outside of the rice is kept dry by airing during harvesting, the rice is not easy to rot, and the rice can be stored in a bag and label mode, so that varieties and specifications can be conveniently searched.
Wherein, the knife used in S1 is a disinfected knife and can be disinfected once every time, thus avoiding the other kinds of potatoes from carrying diseased plants and infecting the diseased plants to other potatoes through contact and ensuring that the original potato seeds are healthy and safe.
Wherein, the bottleneck department of cultivating the growth bud in S2 installs the breather valve, and the temperature that the tissue culture seedling grows between should keep 20 ~ 24 ℃ and illumination intensity is 2600 ~ 3000lx, and the breather valve not only can guarantee that the oxygen volume in the blake bottle is sufficient, and can be fine prevents the pollutant entering for inside tissue culture seedling can healthily grow up, and temperature and required illumination intensity all are the essential factor that can not lack in the growth of tissue culture seedling.
The S3 fertilizer contains diammonium phosphate, chicken manure and potassium sulfate and phoxim particles, provides certain nutrients, and can prevent and control soil insects, and after watering, the nutrients can be better distributed, so that a tamping foundation is laid, and the nutrients are sufficient.
Before the test-tube plantlets in the S5 are transplanted, sterile tweezers are used for clamping cleaned test-tube plantlets and placing the test-tube plantlets into a naphthylacetic acid solution to be soaked for 3-5S, then planting is carried out, the sterile tweezers are used for ensuring that the clamped test-tube plantlets are not infected with germs, and the naphthylacetic acid after filtration and sterilization is used for soaking, so that cell division and expansion can be promoted, and the regulator for plant growth is provided.
Wherein, can take off the membrane after 4 ~ 7d after transplanting in S5, because need water after the test tube seedling transplants, the effect of epiphragma can keep warm and moisturize, provides a comfortable and stable growing environment for the seedling of just transplanting, makes it can the steady weak earlier stage of passing through.
Wherein, the nutrient solution mentioned in S6 is MS nutrient solution, which contains a large amount of nitrate, potassium and ammonium, and in addition, the inorganic salt and ion have higher concentration, which is stable equilibrium solution, and can meet the nutrition and physiological needs of plant cells.
In S7, water and fertilizer supply is stopped one week before harvesting, and stems and leaves on the ground are removed, so that soil can be ensured to be gradually dried, the skins of the potato blocks can be promoted to be gradually hardened, picking work is facilitated, the surfaces of the potato blocks are not easy to peel in the process, the insides of the potato blocks are not polluted, and viruses on the stems and leaves can be prevented from being transferred to tubers.
The first embodiment is as follows:
s1, removing viruses in the plants by a stem tip stripping and heat treatment method, then cutting off the top points of the growing buds by a knife and putting the cut growing buds into a culture medium for cultivation;
s2, recording the date on the bottle for cultivating the growing bud and moving the bottle to a growing room of the tissue culture seedling for cultivating in an aseptic environment;
s3, paving a layer of coal cinder with the thickness of about 4cm on the ground of the insect-proof sterile greenhouse, sterilizing, killing insects, applying fertilizer, paving a layer of sterile cloth on the surface of the coal cinder, and paving a layer of nutrient soil on the top of the coal cinder;
s4, selecting a strong and pollution-free test-tube plantlet with a plant height of 6cm and more than 3 leaves, then putting the test-tube plantlet in a natural air temperature environment for hardening for 4d, taking the test-tube plantlet out of a bottle, and washing off a culture medium on roots of the test-tube plantlet with water;
s5, taking out the washed test-tube plantlets, transplanting the test-tube plantlets into prepared nutrient soil, keeping the plant-row spacing at 5cm, enabling tips of the plantlets to leak out of the ground by 2cm and expose 2 leaves, watering and laminating, and controlling the temperature at 23 ℃ and the humidity at 90%;
s6, after 7 days, regulating and controlling the indoor temperature to be kept at 25 ℃ and the humidity to be kept at 81%, fertilizing by using nutrient solution in the early stage, fertilizing by using fertilizers such as urea, monopotassium phosphate and diammonium phosphate after 30 days, and performing pest control once a week;
s7, when the plants grow to 90 days, 82% of the potato blocks grow to be more than 2.5g and become yellow, the potato blocks can be harvested, the harvested potato blocks are aired for a plurality of days until the potato skins are aged, then the potato blocks are bagged, labeled and moved to a storage warehouse, the temperature in the storage warehouse is kept at 2-4 ℃, and the humidity is 88%.
Example two:
s1, removing viruses in the plants by a stem tip stripping and heat treatment method, then cutting off the top points of the growing buds by a knife and putting the cut growing buds into a culture medium for cultivation;
s2, recording the date on the bottle for cultivating the growing bud and moving the bottle to a growing room of the tissue culture seedling for cultivating in an aseptic environment;
s3, paving a layer of cinder with the thickness of about 5cm on the ground of the insect-proof sterile greenhouse, sterilizing, killing insects, applying fertilizer, paving a layer of sterile cloth on the surface of the cinder, and paving a layer of nutrient soil on the top of the cloth;
s4, selecting a strong and pollution-free test-tube plantlet with a plant height of 6.5cm and more than 3 leaves, then putting the test-tube plantlet in a natural air temperature environment for hardening for 5 days, taking out the test-tube plantlet from a bottle, and washing off a culture medium on roots of the test-tube plantlet with water;
s5, taking out the washed test-tube plantlets, transplanting the test-tube plantlets into prepared nutrient soil, keeping the plant row spacing at 6cm, enabling the tips of the plantlets to leak out of the ground by 2.5cm and expose 2 leaves, watering and laminating, and controlling the temperature at 24 ℃ and the humidity at 93%;
s6, after 8 days, regulating and controlling the indoor temperature to be 27 ℃ and the humidity to be 84%, fertilizing by using nutrient solution in the early stage, fertilizing by using fertilizers such as urea, monopotassium phosphate and diammonium phosphate after 30 days, and performing pest control once a week;
s7, when the plants grow to 100 days, 85% of the potato blocks grow to be more than 2.5g and become yellow, the potato blocks can be harvested, the harvested potato blocks are aired for a plurality of days until the potato skins are aged, then the potato blocks are bagged, labeled and moved to a storage warehouse, the temperature in the storage warehouse is kept at 2-4 ℃, and the humidity is 90%.
Example three:
s1, removing viruses in the plants by a stem tip stripping and heat treatment method, then cutting off the top points of the growing buds by a knife and putting the cut growing buds into a culture medium for cultivation;
s2, recording the date on the bottle for cultivating the growing bud and moving the bottle to a growing room of the tissue culture seedling for cultivating in an aseptic environment;
s3, paving a layer of cinder with the thickness of about 6cm on the ground of the insect-proof sterile greenhouse, sterilizing, killing insects, applying fertilizer, paving a layer of sterile cloth on the surface of the cinder, and paving a layer of nutrient soil on the top of the cloth;
s4, selecting a strong and pollution-free test-tube plantlet with a plant height of 7cm and more than 3 leaves, then putting the test-tube plantlet in a natural air temperature environment for hardening for 6d, taking the test-tube plantlet out of a bottle, and washing off a culture medium on roots of the test-tube plantlet with water;
s5, taking out the washed test-tube plantlets, transplanting the test-tube plantlets into prepared nutrient soil, keeping the plant-row spacing at 7cm, enabling tips of the plantlets to leak out of the ground by 3cm and expose 2 leaves, watering and laminating, and controlling the temperature at 25 ℃ and the humidity at 97%;
s6, after 8 days, regulating and controlling the indoor temperature to be 28 ℃ and the humidity to be 82%, fertilizing by using nutrient solution in the early stage, fertilizing by using fertilizers such as urea, monopotassium phosphate and diammonium phosphate after 30 days, and performing pest control once a week;
s7, when the plants grow to 110d, 90% of the potato blocks grow to be more than 2.5g and become yellow, the potato blocks can be harvested, the harvested potato blocks are aired for a plurality of days until the potato skins are aged, then the potato blocks are bagged, labeled and moved to a storage warehouse, the temperature in the storage warehouse is kept at 2-4 ℃, and the humidity is 94%.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A potato breeder stock breeding matching technology is characterized by comprising the following steps:
s1, removing viruses in the plants by a stem tip stripping and heat treatment method, then cutting off the top points of the growing buds by a knife and putting the cut growing buds into a culture medium for cultivation;
s2, recording the date on the bottle for cultivating the growing bud and moving the bottle to a growing room of the tissue culture seedling for cultivating in an aseptic environment;
s3, paving a layer of coal cinder with the thickness of about 4-6 cm on the ground of the insect-proof sterile greenhouse, sterilizing, killing insects, applying fertilizer, paving a layer of sterile cloth on the surface of the coal cinder, and paving a layer of nutrient soil on the top of the coal cinder;
s4, selecting a strong and pollution-free test-tube plantlet with a plant height of 6-7 cm and more than 3 leaves, then hardening the test-tube plantlet in a natural air temperature environment for 3-6 days, taking out the test-tube plantlet from a bottle, and washing off a culture medium on roots of the test-tube plantlet with water;
s5, taking out the washed test-tube plantlets, transplanting the test-tube plantlets into prepared nutrient soil, keeping the plant-row spacing at 4-8 cm, enabling tips of the plantlets to leak out of the ground by 2-3 cm and exposing 2 leaves, then watering and laminating, and controlling the temperature to be 22-25 ℃ and the humidity to be 95%;
s6, after 7-8 days, regulating and controlling the indoor temperature to be 25-28 ℃ and the humidity to be 80%, fertilizing by using nutrient solution in the early stage, fertilizing by using fertilizers such as urea, monopotassium phosphate and diammonium phosphate after 30 days, and performing pest control once every week;
s7, when the plants grow to 80-120 days, 85% of the potato blocks grow to be more than 2.5g and become yellow, the potato blocks can be harvested, the harvested potato blocks are aired for a plurality of days until the potato skins are aged, then the potato blocks are bagged, labeled and moved to a storage warehouse, the temperature in the storage warehouse is kept at 2-4 ℃, and the humidity is 85%.
2. The potato breeder's stock breeding matching technology of claim 1, which is characterized in that: the knives used in S1 are sterilized knives and each time a cut is made, a sterilization work is performed on the knife.
3. The potato breeder's stock breeding matching technology of claim 1, which is characterized in that: and a vent valve is arranged at the bottle mouth for culturing the growing buds in the S2, the temperature of the tissue culture seedling during growth is kept at 20-24 ℃, and the illumination intensity is 2600-3000 lx.
4. The potato breeder's stock breeding matching technology of claim 1, which is characterized in that: the fertilizer in the S3 contains diammonium phosphate, chicken manure and potassium sulfate with phoxim granules.
5. The potato breeder's stock breeding matching technology of claim 1, which is characterized in that: and (3) before transplanting the test-tube plantlets in the S5, clamping the cleaned test-tube plantlets by using sterile forceps, soaking the test-tube plantlets in a naphthylacetic acid solution for 3-5S, and then planting.
6. The potato breeder's stock breeding matching technology of claim 1, which is characterized in that: and (4) uncovering the film after transplanting in the S5 for 4-7 d.
7. The potato breeder's stock breeding matching technology of claim 1, which is characterized in that: the nutrient solution mentioned in S6 is MS nutrient solution.
8. The potato breeder's stock breeding matching technology of claim 1, which is characterized in that: the supply of water and fertilizer was stopped one week before harvesting and the stems and leaves on the ground were removed in S7.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498842A (en) * | 2011-10-11 | 2012-06-20 | 达州市农业科学研究所 | Method for producing virus-free potato breeder's stock |
| CN105123468A (en) * | 2015-07-27 | 2015-12-09 | 达州市农业科学研究所 | Virus-free potato test tube plantlet rapid propagation method and apparatus |
| CN105210600A (en) * | 2015-07-08 | 2016-01-06 | 甘肃凯凯农业科技发展股份有限公司 | Virus-free basic potato seed hot-house culture method |
| CN106258975A (en) * | 2016-08-24 | 2017-01-04 | 遵义华富生物科技有限公司 | A kind of potato primary stock production method |
| CN207589617U (en) * | 2017-11-22 | 2018-07-10 | 宁洱鑫泰商贸有限公司 | The bletilla striata tames seedling bed |
-
2021
- 2021-10-22 CN CN202111229895.2A patent/CN113940246A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498842A (en) * | 2011-10-11 | 2012-06-20 | 达州市农业科学研究所 | Method for producing virus-free potato breeder's stock |
| CN105210600A (en) * | 2015-07-08 | 2016-01-06 | 甘肃凯凯农业科技发展股份有限公司 | Virus-free basic potato seed hot-house culture method |
| CN105123468A (en) * | 2015-07-27 | 2015-12-09 | 达州市农业科学研究所 | Virus-free potato test tube plantlet rapid propagation method and apparatus |
| CN106258975A (en) * | 2016-08-24 | 2017-01-04 | 遵义华富生物科技有限公司 | A kind of potato primary stock production method |
| CN207589617U (en) * | 2017-11-22 | 2018-07-10 | 宁洱鑫泰商贸有限公司 | The bletilla striata tames seedling bed |
Non-Patent Citations (1)
| Title |
|---|
| 童霄丽: "脱毒马铃薯原原种繁育技术", 《农民科技培训》 * |
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