CN113930321A

CN113930321A - Dissociation assembly, tissue dissociation tube, dissociation device and tissue dissociation method

Info

Publication number: CN113930321A
Application number: CN202111341321.4A
Authority: CN
Inventors: 张梦超; 倪鸣; 徐炳坤
Original assignee: Qingdao Huada Zhizao Technology Co ltd
Current assignee: Qingdao Huada Zhizao Technology Co ltd
Priority date: 2021-11-12
Filing date: 2021-11-12
Publication date: 2022-01-14
Anticipated expiration: 2041-11-12
Also published as: CN113930321B

Abstract

The application provides a dissociation subassembly, tissue dissociation pipe, dissociation device and tissue dissociation method, and the subassembly that dissociates includes base, rotation piece and closing cap spare. The base includes first connecting portion, second connecting portion and a plurality of piece that dissociates, first connecting portion and second connecting portion enclose respectively and enclose into first accepting the chamber and the chamber is accepted to the second, a plurality of pieces that dissociate set up and all connect in the first inner wall of accepting the chamber along the first circumference of accepting the chamber, the flowing groove has been seted up between two adjacent pieces that dissociate, it includes the rotation base member to rotate the piece, the cutting member that connector and spiral bending extend, rotate the inner wall clearance fit that the base member rotates to be connected and accept the chamber with the second with second connecting portion, the cutting member is connected in the connector and with the first inner wall clearance fit who accepts the chamber, a plurality of pieces that dissociate enclose locate cutting member periphery and set up with cutting member interval, the closure piece is connected with first connecting portion detachably. The dissociation assembly can perform continuous gradient dissociation on tissues, the grinding speed is high, and the obtained single cell suspension has good dispersibility.

Description

Dissociation assembly, tissue dissociation tube, dissociation device and tissue dissociation method

Technical Field

The application relates to the technical field of tissue separation devices, in particular to a dissociation assembly, a tissue dissociation tube, a dissociation device and a tissue dissociation method.

Background

The single cell suspension is a biological tissue of dispersed single cells released by destroying the connection between cells in the tissue, has the characteristics of sufficient cell dispersion, high activity and the like, and is the basis for researching biological cells. Among them, the dispersibility of cells in a single-cell suspension directly affects the quality of the single-cell suspension. However, the existing device for preparing single cell suspension has high cost, and the dispersibility of the single cell suspension obtained by aiming at the tissue with stronger viscosity is not ideal.

Disclosure of Invention

In view of the above, there is a need to provide a dissociation assembly, a tissue dissociation tube, a dissociation device and a tissue dissociation method, so as to solve the technical problem of obtaining a single-cell suspension with high dispersibility at low cost.

An embodiment of the present application provides a dissociation assembly, including:

the base comprises a first connecting part and a second connecting part which are mutually communicated, the first connecting part is hollow and encloses a first accommodating cavity, the second connecting part is hollow and encloses a second accommodating cavity communicated with the first accommodating cavity, the first accommodating cavity and the second accommodating cavity jointly penetrate through the base, the base further comprises a plurality of dissociation parts which are convexly arranged on the first connecting part and are positioned in the first accommodating cavity, each dissociation part is connected to the inner wall of the first accommodating cavity, the dissociation parts are arranged along the circumferential direction of the first accommodating cavity, and a flow groove is formed between every two adjacent dissociation parts;

the rotating body is sleeved in the base and is rotatably connected with the base, the rotating body comprises a rotating base body, a connecting body and a cutting body, the rotating base body is rotatably connected with the second connecting part and is in clearance fit with the inner wall of the second accommodating cavity, the connecting body extends into the first accommodating cavity from the rotating base body and can synchronously rotate with the rotating base body, the cutting body is connected with the connecting body and is in clearance fit with the inner wall of the first accommodating cavity, the dissociation parts are arranged around the periphery of the cutting body at intervals with the cutting body, and the cutting body is spirally bent and extends from one end of the connecting body close to the rotating base body to the direction of one end far away from the second connecting part;

the cover piece is detachably connected with the first connecting part and is provided with a cover groove, and when the cover piece is connected to the base, the cover groove is communicated with the first accommodating cavity.

In some embodiments, the first receiving cavity and the second receiving cavity are coaxially disposed, and a distance between an inner wall of the first receiving cavity and the connecting body gradually decreases from the first connecting portion to the second connecting portion.

In some embodiments, the dissociation assembly further comprises:

and the clamping piece is connected to one end of the rotating base body, which is far away from the connecting body, protrudes out of the second accommodating cavity and is used for clamping one end of the second connecting part, which is far away from the first connecting part.

In some embodiments, a plurality of the dissociation parts enclose to form a dissociation pool in a shape of a cone barrel, and the aperture of the dissociation pool gradually increases towards a direction away from the second connecting part.

In some embodiments, an inner diameter of the second connecting portion is smaller than an inner diameter of the first connecting portion, an end surface of the second connecting portion, which is close to the first connecting portion, forms an end surface that divides the first receiving cavity and the second receiving cavity, and the dissociation members extend from the end surface in a direction away from the second connecting portion.

In some embodiments, the dissociation part includes a top surface opposite to the end surface and disposed at an interval, and a first dissociation side surface and a second dissociation side surface connected between the top surface and the end surface, the first dissociation side surface and the second dissociation side surface intersect to form a cutting edge, one sides of the first dissociation side surface and the second dissociation side surface, which are far away from the cutting edge, are respectively connected with an inner wall of the first accommodating cavity, the area of the first dissociation side surface is larger than that of the second dissociation side surface, and the flow groove is formed between the first dissociation side surface and the adjacent second dissociation side surface; or, dissociate the piece include with the relative and top surface that the interval set up of terminal surface, and connect the top surface with first dissociation side, second dissociation side and cutting plane between the terminal surface, the cutting plane is connected first dissociation side with between the second dissociation side, first dissociation side is kept away from with the second dissociation side one side of cutting plane with first interior wall connection who accepts the chamber, the area of first dissociation side is greater than the second dissociation side, first dissociation side and adjacent form between the second dissociation side the flowing groove.

In some embodiments, the cutting body includes a cutting portion located in the dissociation chamber and a guide portion extending from a side of the cutting portion away from the rotating base to outside the dissociation chamber, the guide portion is used for guiding the tissue into the dissociation chamber when the cover member is connected with the base, the guide portion covers a portion of the dissociation member in an orthographic projection of a radial plane of the dissociation chamber, and the cutting portion includes a radial portion extending radially from the connecting portion.

In some embodiments, the cutting body includes a cutting portion located in the dissociation pool and a guiding portion extending from a side of the cutting portion away from the rotating base body to an outside of the dissociation pool, the guiding portion is used for guiding a tissue into the dissociation pool when the cover component is connected with the base, the guiding portion covers a part of the dissociation piece in an orthographic projection of a radial plane of the dissociation pool, the cutting portion includes a radial portion extending from the connecting body in a radial direction and a circumferential portion extending from one end of the radial portion away from the connecting body in a circumferential bending manner of the connecting body, and the circumferential portion is smoothly connected with the radial portion.

In some embodiments, a single cutting portion is housed in the dissociation chamber, and the guide portion and the cutting portion do not overlap each other in an orthographic projection of the radial plane; or at least two cutting parts are symmetrically arranged in the dissociation pool relative to the connecting body.

In some embodiments, a circumferential fixing groove is formed in one side of the rotating base body adjacent to the connecting body, and the rotating member further includes a sealing body, which is sleeved on and protrudes from the fixing groove.

In some embodiments, the connecting body is provided with a cutting groove extending in a direction from the first connecting portion to the second connecting portion, and a plurality of flow holes each extending inward from the connecting body outer peripheral wall and communicating with the cutting groove.

The embodiment of the present application further provides a dissociation device, including:

in the dissociation assembly of any of the above embodiments, the rotation base is provided with a rotation groove, the rotation groove has an opening, the opening is disposed away from the connecting body, the rotation member further includes a plurality of locking bodies, each locking body is connected to an inner wall of the rotation groove, and the plurality of locking bodies are disposed along a circumferential direction of the rotation groove;

and the driving piece is clamped and matched with the plurality of locking bodies to drive the rotating piece to rotate.

The dissociation assembly and the dissociation device can dissociate tissues to obtain single cell suspension, wherein the first accommodating cavity is used for accommodating the tissues, the dissociation parts are circumferentially connected to the inner wall of the first accommodating cavity to form a continuous step-shaped structure, when the tissues are dissociated, the rotating base body rotates in the second accommodating cavity and drives the cutting body positioned on the connecting body to rotate relative to the dissociation parts, the tissues positioned in the first accommodating cavity are continuously and directionally pushed to the step-shaped structure formed by the dissociation parts, and then the tissues are extruded and ground to obtain the single cell suspension. The dissociation component can perform continuous gradient dissociation on tissues, has high contact speed and large grinding force, and the obtained single cell suspension has good dispersibility.

The embodiment of the present application further provides a tissue dissociation tube, including:

the accommodating tube is provided with an opening end and a cavity for accommodating tissues to be dissociated, and the opening end is communicated with the cavity;

a base including a first connection part and a second connection part which are communicated with each other, the second connection part being located outside the containing pipe, the first connecting part extends from the second connecting part to the cavity and covers the opening end, the first connecting part is detachably connected with the opening end, the first connecting part is hollow and encloses a first accommodating cavity communicated with the cavity, the second connecting part is hollow and encloses a second containing cavity communicated with the first containing cavity, the first containing cavity and the second containing cavity jointly penetrate through the base, the base further comprises a plurality of dissociation pieces which are convexly arranged on the first connecting part and are positioned in the first containing cavity, each dissociation piece is connected to the inner wall of the first containing cavity, the dissociation parts are arranged along the circumferential direction of the first accommodating cavity, and a flow groove is formed between every two adjacent dissociation parts;

rotate the piece, the cover is located in the base and with the base rotationally connects, including rotating base member, connector and cutting body, rotate the base member rotationally connect in the second connecting portion and with the inner wall clearance fit in chamber is acceptd to the second, the connector certainly rotate the base member and extend to first acceping the intracavity, and with it can rotate in step to rotate the base member, cut the body connect in the connector and with first inner wall clearance fit who accepts the chamber is a plurality of it encloses to dissociate the piece and locates cut the body periphery and with the cutting body interval sets up, cut the body certainly the connector is close to the one end orientation of rotating the base member is kept away from the direction of the one end of second connecting portion is the crooked extension of heliciform.

Above-mentioned tissue dissociation pipe, the outside manual operation pole that sets up of accessible, the grinding of a small amount of tissue is accomplished in manual work, uses more in a flexible way, easier control.

The embodiment of the present application further provides a tissue dissociation method for a tissue dissociation tube, including:

adding tissue into the accommodating tube and covering the base on the accommodating tube, wherein at least part of the tissue is accommodated between the rotating piece and the dissociation piece;

providing a manual operating rod, clamping the operating rod with the rotating base body, and meshing the manual operating rod with the rotating base body;

rotate manual operation pole is in order to drive the rotation base member, it drives to rotate the base member the cutting body is relative dissociate the piece rotate and with dissociate the piece and mutually support in order to realize right the dissociation of tissue.

The tissue dissociation method of the tissue dissociation tube can finish grinding of a small amount of tissues in a manual operation mode, and is high in flexibility and convenient and fast to operate.

The embodiment of the present application further provides a tissue dissociation method for a dissociation assembly, including:

adding tissue to the cover member and attaching the base to the cover member, at least a portion of the tissue being contained between the rotating member and the severing member;

providing a driving part, wherein the driving part comprises a driving motor and a driving output shaft connected with the driving motor, and connecting the driving output shaft with the rotating base body;

utilize driving motor drive output shaft to by drive output shaft drives rotate the piece relative the base is rotatory, the cutting body in rotatory in-process with the cooperation of dissociation piece is in order to realize to the dissociation of tissue.

According to the tissue dissociation method of the dissociation assembly, continuous gradient dissociation of the tissue is realized through the provided driving piece, the contact speed is high, the grinding force is large, and the obtained tissue grinding product has good dispersibility.

Drawings

Fig. 1 is a schematic structural diagram of a dissociation device according to an embodiment of the present disclosure.

Fig. 2 is an exploded view of a dissociation device according to an embodiment of the present disclosure.

Fig. 3 is a schematic cross-sectional view of a base along a direction iii according to an embodiment of the present disclosure.

Fig. 4 is a schematic cross-sectional view of the base and the rotating member along the direction iii according to an embodiment of the present application.

Fig. 5 is a schematic structural diagram of a rotating member according to an embodiment of the present application.

Fig. 6 is an exploded view of a dissociation device according to an embodiment of the present disclosure.

Fig. 7 is a schematic top view of the base and the rotating member according to the second embodiment of the present application.

Fig. 8 is an exploded view of a three dissociation device according to an embodiment of the present disclosure.

Fig. 9 is a schematic top view of the base and the rotating member according to the third embodiment of the present application.

Fig. 10 is a bottom view of a rotating member according to an embodiment of the present application.

Fig. 11 is a flowchart of a tissue dissociation method of a tissue dissociation tube according to a sixth embodiment of the present application.

Fig. 12 is a flowchart of a tissue dissociation method of a dissociation module according to a seventh embodiment of the present disclosure.

Description of the main elements

Dissociation assembly

10, 20, 30

Base 11

First receiving cavity 11a

Second accommodation chamber 11b

End face 11c

First connection portion 111

Inner barrel 1111

Outer cylinder 1112

Locking thread segment 1112a

Fixing part 1113

Second connecting part 112

Detaching member 113

Dissociation pool 113a

Top surface 1131

First dissociation side 1132

Second dissociating side 1133

Cutting edge 1134

Cutting surface 1135

Flow channel 114

Rotor 12

Rotating base 121

Fixing groove 1211

Turning groove 1212

Connecting body 122

Cutting groove 1221

Flow aperture 1222

Cutting body 123

Cutting part 1231

Radial portion 1231a

Circumferential portion 1231b

Guide part 1232

Sealing body 124

Locking body 125

Raised grain 126

Closure member 13

Cover groove 131

Turning thread segment 1311

Retaining member 14

Detailed Description

Reference will now be made in detail to embodiments of the present application, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative and are only for the purpose of explaining the present application and are not to be construed as limiting the present application.

In the description of the present application, it is to be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the present application and for simplicity in description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed in a particular orientation, and be operated in a particular manner, and are not to be construed as limiting the present application. Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, features defined as "first", "second", may explicitly or implicitly include one or more of the described features. In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.

In the description of the present application, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; may be mechanically connected, may be electrically connected or may be in communication with each other; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.

In this application, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact of the first and second features, or may comprise contact of the first and second features not directly but through another feature in between. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly above and obliquely above the second feature, or simply meaning that the first feature is at a lesser level than the second feature.

The following disclosure provides many different embodiments or examples for implementing different features of the application. In order to simplify the disclosure of the present application, specific example components and arrangements are described below. Of course, they are merely examples and are not intended to limit the present application. Moreover, the present application may repeat reference numerals and/or letters in the various examples, such repetition is for the purpose of simplicity and clarity and does not in itself dictate a relationship between the various embodiments and/or configurations discussed. In addition, examples of various specific processes and materials are provided herein, but one of ordinary skill in the art may recognize applications of other processes and/or use of other materials.

The present embodiments provide a dissociation assembly for dissociating tissue to form a single cell suspension, the dissociation assembly comprising a base, a rotating member, and a closure member. The base includes first connecting portion and the second connecting portion that communicate each other, first connecting portion are the cavity form and enclose into first accepting the chamber, the second connecting portion are the cavity form and enclose into and first accepting the second of chamber intercommunication and accept the chamber, first accepting the chamber and the second accepts the chamber and run through the base jointly, the base still includes protruding a plurality of pieces of dissociation of locating first connecting portion and being located first accepting the intracavity, every piece of dissociation is connected in the first inner wall of accepting the chamber, and a plurality of pieces of dissociation set up along the first circumference of accepting the chamber, the flowing groove has been seted up between two adjacent pieces of dissociation. Rotate the piece cover and locate in the base and rotationally be connected with the base, it includes the rotation base member to rotate the piece, the connector and the cutting body, it rotationally connects in the second connecting portion and accepts the inner wall clearance fit in chamber with the second to rotate the base member, the connector extends to first intracavity of acceping from rotating the base member, and can rotate in step with the rotation base member, the cutting body is connected in the connector and with first inner wall clearance fit who accepts the chamber, a plurality of pieces of dissociation enclose locate the cutting body periphery and with the cutting body interval setting, the cutting body is the crooked extension of heliciform from the direction that the one end of the second connecting portion was kept away from to the one end orientation that the connector is close to the rotation base member. The cover piece is detachably connected with the first connecting part, the cover piece is provided with a cover groove, and when the cover piece is connected to the base, the cover groove is communicated with the first accommodating cavity.

The embodiment of the application also provides an dissociation device, which comprises a driving piece and the dissociation assembly of any one of the embodiments. The rotation base member is equipped with the rotation recess, rotates the recess and has an opening, and the opening deviates from the connector setting, rotates the piece and still includes a plurality of locking bodies, and a plurality of locking body circumferential connection are in the inner wall that rotates the recess. The driving piece is clamped and matched with the plurality of locking bodies to drive the rotating piece to rotate.

The embodiment of the application also provides a tissue dissociation tube, a tissue dissociation method of the tissue dissociation tube and a tissue dissociation method of the dissociation assembly. The following describes in detail various embodiments of the present application with reference to the drawings.

Example one

Referring to fig. 1 to 3, the dissociation assembly 10 in the present embodiment includes a base 11, a rotation member 12 and a cover member 13. The base 11 includes a first connecting portion 111 and a second connecting portion 112 that are connected to each other, the first connecting portion 111 is hollow and encloses a first receiving cavity 11a, the second connecting portion 112 is hollow and encloses a second receiving cavity 11b that is connected to the first receiving cavity 11a, the first receiving cavity 11a and the second receiving cavity 11b jointly penetrate the base 11, the base 11 further includes a plurality of dissociation parts 113 protruding from the first connecting portion 111 and located in the first receiving cavity 11a, each dissociation part 113 is connected to an inner wall of the first receiving cavity 11a, the plurality of dissociation parts 113 are disposed along a circumferential direction of the first receiving cavity 11a, and a flow groove 114 is disposed between two adjacent dissociation parts 113. The rotating member 12 is sleeved in the base 11 and rotatably connected to the base 11, the rotating member 12 includes a rotating base 121, a connecting body 122 and a cutting body 123, the rotating base 121 is rotatably connected to the second connecting portion 112 and is in clearance fit with an inner wall of the second accommodating cavity 11b, the connecting body 122 extends from the rotating base 121 to the first accommodating cavity 11a and can rotate synchronously with the rotating base 121, in this embodiment, the connecting body 122 and the rotating base 121 are coaxially disposed, the cutting body 123 is connected to the connecting body 122 and is in clearance fit with an inner wall of the first accommodating cavity 11a, the plurality of separating members 113 are surrounded on an outer periphery of the cutting body 123 and are spaced from the cutting body 123, and the cutting body 123 is spirally bent and extends from one end of the connecting body 122 close to the rotating base 121 toward one end far away from the second connecting portion 112. The cover member 13 is detachably connected to the first connecting portion 111, the cover member 13 has a cover slot 131, when the cover member 13 is connected to the base 11, the cover slot 131 is communicated with the first receiving cavity 11a, and the cover slot 131 can receive the tissue to be dissociated.

In this embodiment, the base 11, the rotating base 121 and the connecting body 122 are all cylindrical structures.

Referring to fig. 3, in the present embodiment, the first connecting portion 111 includes an inner cylinder 1111, an outer cylinder 1112, and a fixing member 1113. The inner cylinder 1111 is connected with the disconnecting part 113 and encloses a first receiving cavity 11a, the outer cylinder 1112 is sleeved on the inner cylinder 1111 and is arranged at an interval with the inner cylinder 1111, the closing cover part 13 is detachably sleeved between the inner cylinder 1111 and the outer cylinder 1112, and the fixing part 1113 is connected between the inner cylinder 1111 and the outer cylinder 1112.

In this way, the first connection portion 111 is formed in a sleeve form, so that the cover member 13 is detachably sleeved between the inner cylinder 1111 and the outer cylinder 1112, and the overall occupied area of the dissociation assembly 10 can be reduced.

Referring to fig. 2 and 3, in some embodiments, a locking thread segment 1112a is disposed on a side of the outer cylinder 1112 facing the inner cylinder 1111, a rotating thread segment 1311 is disposed on an outer wall of the cap groove 131, and the rotating thread segment 1311 is adapted to the locking thread segment 1112 a.

Therefore, the cover member 13 and the base 11 can be detachably connected by adopting a threaded connection mode, and the use is convenient and the manufacturing cost is low.

In this embodiment, the first receiving cavity 11a and the second receiving cavity 11b are coaxially disposed, and a distance between an inner wall of the first receiving cavity 11a and the connecting body 122 gradually decreases from the first connecting portion 111 to the second connecting portion 112.

The first receiving cavity 11a forms an inverted cone structure, so as to receive the tissue to be separated conveniently, and prevent the tissue from running off from the second connecting portion 112 during the separation process.

In this embodiment, the dissociation parts 113 enclose a conical barrel-shaped dissociation pool 113a, and the aperture of the dissociation pool 113a gradually increases toward a direction away from the second connection part 112.

In this embodiment, the inner diameter of the second connecting portion 112 is smaller than the inner diameter of the first connecting portion 111, an end surface 11c is formed at an end of the second connecting portion 112 close to the first connecting portion 111 to divide the first receiving cavity 11a and the second receiving cavity 11b, and the plurality of dissociation pieces 113 extend from the end surface 11c toward a direction away from the second connecting portion 112.

With reference to fig. 3, in the present embodiment, the dissociation element 113 includes a top surface 1131 opposite to the end surface 11c and disposed at an interval, and a first dissociation side surface 1132 and a second dissociation side surface 1133 connected between the top surface 1131 and the end surface 11c, the first dissociation side surface 1132 and the second dissociation side surface 1133 intersect to form a cutting edge 1134, one side of the first dissociation side surface 1132 and one side of the second dissociation side surface 1133 far from the cutting edge 1134 are respectively connected to an inner wall of the first receiving cavity 11a, an area of the first dissociation side surface 1132 is larger than that of the second dissociation side surface 1133, and a flow groove 114 is formed between the first dissociation side surface 1132 and the adjacent second dissociation side surface 1133. The cross section of the dissociation part 113 on the radial plane of the dissociation pool 113a is triangular.

In this embodiment, the cutting elements 123 are three and evenly distributed at intervals along the circumferential direction of the connecting body 122, each cutting element 123 includes a cutting portion 1231 located in the dissociation pool 113a and a guiding portion 1232 extending from a side of the cutting portion 1231 away from the rotating base 121 to the outside of the dissociation pool 113a, the guiding portion 1232 is used for guiding the tissue into the dissociation pool 113a when the cover 13 is connected with the base 11, the guiding portion 1232 covers a part of the dissociation element 113 in a forward projection of a radial plane of the dissociation pool 113a, and the cutting portion 1231 only includes a radial portion 1231a extending from the connecting portion in a radial direction.

In this embodiment, the gap between each cutting portion 1231 and the inner wall of the first accommodating cavity 11a is in a range of 0.05mm to 0.08mm, and each cutting portion 1231 extends in a spiral bending manner along the axial direction of the connecting body 122, so that an arc-shaped transition space is formed between each cutting portion 1231 and the inner wall of the first accommodating cavity 11a, and a larger piece of tissue can be squeezed and ground.

In another preferred embodiment of the present invention, only a single cutting body may be provided, in which the cutting part 1231 is accommodated in the dissociation tank 113a, and the orthogonal projections of the guide part 1232 and the cutting part 1231 on the radial plane do not overlap each other; alternatively, at least two cutting bodies are provided, and the cutting portions 1231 thereof are symmetrically disposed in the dissociation tank 113a with respect to the connection body 122.

Referring to fig. 4 and fig. 5, in the present embodiment, the dissociation assembly 10 further includes a holding member 14, the holding member 14 is connected to an end of the rotation base 121 far from the connecting body 122, and the holding member 14 protrudes out of the second receiving cavity 11b and is used for holding an end of the second connecting portion 112 far from the first connecting portion 111.

Thus, the retaining member 14 can prevent the rotating member 12 from moving from the second connecting portion 112 to the first connecting portion 111 when the rotating member 12 rotates relative to the base 11, so as to prevent the rotating member 12 from separating from the base 11.

In this embodiment, a circumferential fixing groove 1211 is formed on a side of the rotating base 121 adjacent to the connecting body 122, and the rotating member 12 further includes a sealing body 124, wherein the sealing body 124 is sleeved on and protrudes from the fixing groove 1211.

In some embodiments, the sealing body 124 may be a plastic sealing ring.

In this embodiment, the gap between the rotating base 121 and the inner wall of the second accommodating cavity 11b is between 0.03mm and 0.1mm, so that the rotating element 12 and the base 11 form an effective rotating pair to meet the requirement of high-speed rotation.

With continued reference to fig. 5, in the present embodiment, the connecting body 122 is provided with a cutting slot 1221 and a plurality of flow holes 1222, the cutting slot 1221 extends along the direction from the first connecting portion 111 to the second connecting portion 112, and each flow hole 1222 extends inward from the outer peripheral wall of the connecting body 122 and communicates with the cutting slot 1221.

In this way, by providing the cutting grooves 1221 and the plurality of flow holes 1222 communicating with the cutting grooves 1221, a circulation flow of the tissue can be formed between the cutting grooves 1221 and the plurality of flow holes 1222, and the dispersibility of the tissue being ground can be improved.

In this embodiment, the rotating member 12 is further provided with a plurality of raised grains 126 for increasing the resolving power to the tissue, each raised grain 126 is disposed on the surfaces of the connecting body 122 and the cutting body 123, and the plurality of raised grains 126 are disposed at equal intervals along the axial direction of the connecting body 122.

The use of the dissociation assembly 10 described above is generally as follows:

first, tissue is placed in the first receiving cavity 11a, and the closing member 13 is rotated clockwise to fix the closing member 13 between the outer cylinder 1112 and the inner cylinder 1111; then, the second connecting portion 112 of the rotating member 12 is driven by an external driving member to rotate in the second receiving cavity 11b, so as to drive the cutting member 123 on the connecting member 122 to rotate in the first receiving cavity 11a, and during the rotation process, the tissue in the dissociation tank 113a is cut and ground between the plurality of cutting portions 1231 and the cutting edge 1134 for a plurality of times; after a preset time; closing the drive member, inverting the dissociation assembly 10, and rotating the cover member 13 counterclockwise to separate the cover member 13 from the first connection portion 111; finally, the single cell suspension formed was decanted.

The dissociation assembly 10 can dissociate the tissue to obtain a single cell suspension, wherein the first receiving cavity 11a is used for receiving the tissue, the plurality of dissociation components 113 are circumferentially connected to the inner wall of the first receiving cavity 11a to form a continuous step-shaped structure, when dissociating the tissue, the rotating substrate 121 rotates in the second receiving cavity 11b and drives the cutting body 123 on the connecting body 122 to rotate relative to the plurality of dissociation components 113, the tissue in the first receiving cavity 11a is continuously and directionally pushed to the step-shaped structure formed by the plurality of dissociation components 113, and then the tissue is squeezed and ground to obtain the single cell suspension. The dissociation component 10 can perform continuous gradient dissociation on tissues, has high contact speed and large grinding force, and the obtained single cell suspension has good dispersibility.

Example two

Referring to fig. 6 and 7, the dissociation assembly 20 of the present embodiment includes a base 11, a rotation member 12 and a cover member 13, compared with the first embodiment.

In this embodiment, the dissociation piece 113 includes the top surface 1131 that is relative and the interval setting with the terminal surface 11c, and connect the first side 1132, the second side 1133 and the cutting plane 1135 of dissociating between top surface 1131 and terminal surface 11c, the cutting plane 1135 is connected between the first side 1132 and the second side 1133 of dissociating, the first side 1132 and the second side 1133 of dissociating keep away from the cutting plane 1135 with the first inner wall of accepting the chamber 11a be connected, the area of the first side 1132 of dissociating is greater than the second side 1133 of dissociating, form the flowing groove 114 between the first side 1132 and the adjacent second side 1133 of dissociating. Wherein the cross section of the dissociation part 113 in the radial plane of the dissociation pool 113a is trapezoidal.

In this embodiment, the cutting element 123 is one, and the cutting portion 1231 includes a radial portion 1231a extending from the connecting body 122 in the radial direction and a circumferential portion 1231b extending from one end of the radial portion 1231a away from the connecting body 122 in a bending manner in the circumferential direction of the connecting body 122, and the circumferential portion 1231b is smoothly connected to the radial portion 1231 a. In the present embodiment, the cutting part 1231 is housed in the dissociation tank 113a, and the orthogonal projections of the guide part 1232 and the cutting part 1231 on the radial plane do not overlap each other.

In this embodiment, the connecting body 122 is not provided with the cutting grooves 1221 and the plurality of flow holes 1222, but it is not excluded that corresponding structures are formed on the connecting body.

EXAMPLE III

Referring to fig. 8 and 9, compared to the embodiment, the dissociation assembly 30 of the present embodiment includes a base 11, a rotating member 12, a covering member 13 and a cutting surface 1135, in the present embodiment, two cutting bodies 123 are symmetrically disposed on the connecting body 122, and the cutting portion 1231 includes a radial portion 1231a extending from the connecting body 122 along the radial direction and a circumferential portion 1231b extending from one end of the radial portion 1231a far away from the connecting body 122 along the circumferential direction of the connecting body 122, and the circumferential portion 1231b is smoothly connected to the radial portion 1231 a.

It should be noted that the specific structures of the dissociation part 113 and the cutting body 123 in the first to third embodiments may be changed according to the processing and use requirements.

Example four

The present embodiment provides an dissociation apparatus (not shown), which includes a driving member and any one of the

dissociation assemblies

10, 20, and 30. Referring to fig. 10, fig. 10 is a schematic bottom view of a rotating member 12 according to a first embodiment of the present application, wherein the structures of the rotating bases 121 of the rotating member 12 according to the first embodiment, the second embodiment and the third embodiment are the same, and the rotating member 12 according to the first embodiment is taken as an example for description. The rotating base 121 is provided with a rotating groove 1212, the rotating groove 1212 has an opening, the opening is disposed away from the connecting body 122, the rotating member 12 further includes a plurality of locking bodies 125, each locking body 125 is connected to an inner wall of the rotating groove 1212, the plurality of locking bodies 125 are disposed along a circumferential direction of the rotating groove 1212, and the driving member and the plurality of locking bodies 125 are clamped and matched to drive the rotating member 12 to rotate.

In some embodiments, the driving member may be a driving rod engaged with the plurality of locking bodies 125, and the driving member is connected to a driving motor through the driving rod, or may be a driving motor directly, and an output end of the driving motor is engaged with the plurality of locking bodies 125.

The dissociation component 10 in the dissociation device can perform continuous gradient dissociation on tissues, the contact speed is high, the grinding force is high, and the obtained single cell suspension has good dispersibility. Meanwhile, the driving member is engaged with the plurality of locking bodies 125 in the rotating member 12 to drive the rotating member 12 to rotate, and the structure is simple and the use is convenient.

EXAMPLE five

The present embodiment provides a tissue dissociation tube (not shown), which includes a receiving tube, a base 11 and a rotating member 12. The containing tube has a structure similar to that of the sealing cover in the above embodiment, and has an open end and a cavity for containing the tissue to be dissociated, and the open end is communicated with the cavity.

The base 11 is similar to the base in any of the above embodiments, and includes a first connection portion 111 and a second connection portion 112 that are communicated with each other, the second connection portion 112 is located outside the accommodating tube, the first connection portion 111 extends from the second connection portion 112 into the cavity and covers the opening end, and the first connection portion 111 is detachably connected to the opening end. The first connecting portion 111 is hollow and encloses into a first accommodating cavity 11a communicated with the cavity, the second connecting portion 112 is hollow and encloses into a second accommodating cavity 11b communicated with the first accommodating cavity 11a, the first accommodating cavity 11a and the second accommodating cavity 11b jointly penetrate through the base 11, the base 11 further comprises a plurality of dissociation pieces 113 protruding from the first connecting portion 111 and located in the first accommodating cavity 11a, each dissociation piece 113 is connected to the inner wall of the first accommodating cavity 11a, the dissociation pieces 113 are arranged along the circumferential direction of the first accommodating cavity 11a, and a flow groove 114 is formed between two adjacent dissociation pieces 113.

The rotating member 12 is similar to the base structure of any of the above embodiments, and is sleeved in the base 11 and rotatably connected to the base 11, the rotating member 12 includes a rotating base 121, a connecting member 122 and a cutting member 123, the rotating base 121 is rotatably connected to the second connecting portion 112 and is in clearance fit with the inner wall of the second accommodating cavity 11b, the connecting member 122 extends from the rotating base 121 to the first accommodating cavity 11a, and can rotate synchronously with the rotating base 121, in this embodiment, the connecting body 122 and the rotating base 121 are coaxially disposed, the cutting body 123 is connected to the connecting body 122 and is in clearance fit with the inner wall of the first accommodating cavity 11a, the plurality of dissociation pieces 113 are disposed around the periphery of the cutting body 123 and are spaced from the cutting body 123, and the cutting body 123 is spirally bent and extended from one end of the connecting body 122 close to the rotating base 121 toward one end far away from the second connecting portion 112 (see fig. 1 to 3). The accommodating tube is used for accommodating tissues, the accommodating tube is movably connected with the first connecting part 111 and has an opening end, and when the base 11 is covered on the opening, the part of the first connecting part 111 extends into the accommodating tube.

In this embodiment, the accommodating tube and the base 11 are movably connected or screwed in a clamping manner. During the operation, the rotation of the piece is rotated in the manual control of the manual operation pole manual control that the accessible outside set up, and manual work accomplishes the grinding of a small amount of organizations, uses more in a flexible way, and is controlled more easily.

EXAMPLE six

Referring to fig. 11, the present embodiment provides a tissue dissociation method of the tissue dissociation tube, including:

s11, adding tissue into the containing tube, covering the base on the containing tube, and at least partially containing the tissue between the rotating piece and the dissociation piece.

And S12, providing a manual operating rod, clamping the operating rod with the rotating base body, and engaging the manual operating rod with the rotating base body.

S13, rotating the manual operating rod to drive the rotating base body, and rotating the base body to drive the cutting body to rotate relative to the dissociation piece and to mutually cooperate with the dissociation piece to realize the dissociation of the tissue.

EXAMPLE seven

Referring to fig. 12, the present embodiment provides a tissue dissociation method using the dissociation module of any one of the above embodiments, including:

s21, adding tissue into the cover element and covering the base on the cover element, at least part of the tissue is contained between the rotating element and the dissociation element.

And S22, providing a driving piece, wherein the driving piece comprises a driving motor and a driving output shaft connected with the driving motor, and connecting the driving output shaft with the rotating base body.

S23, the driving motor is used for driving the driving output shaft, the driving output shaft drives the rotating piece to rotate relative to the base, and the cutting body is matched with the dissociation piece in the rotating process to realize the dissociation of the tissue.

It will be evident to those skilled in the art that the present application is not limited to the details of the foregoing illustrative embodiments, and that the present application may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the application being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present application and not for limiting, and although the present application is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present application without departing from the spirit and scope of the technical solutions of the present application.

Claims

1. A dissociation assembly, comprising:

2. The dissociation assembly of claim 1,

the first accommodating cavity and the second accommodating cavity are coaxially arranged, and the distance between the inner wall of the first accommodating cavity and the connecting body is gradually reduced from the first connecting part to the second connecting part.

3. The dissociation assembly of claim 1, wherein the dissociation assembly further comprises:

4. The dissociation assembly of claim 1,

the dissociation parts enclose to form a conical barrel-shaped dissociation pool, and the aperture of the dissociation pool is gradually increased towards the direction far away from the second connecting part.

5. The dissociation assembly of claim 4, wherein the second connection portion has an inner diameter smaller than that of the first connection portion, an end surface of the second connection portion adjacent to the first connection portion is formed to divide the first receiving cavity and the second receiving cavity, and the dissociation members extend from the end surface in a direction away from the second connection portion.

6. The dissociation assembly of claim 5,

the dissociation part comprises a top surface, a first dissociation side surface and a second dissociation side surface, wherein the top surface is opposite to the end surface and is arranged at an interval, the first dissociation side surface and the second dissociation side surface are connected between the top surface and the end surface, the first dissociation side surface and the second dissociation side surface are intersected to form a cutting edge, one sides of the first dissociation side surface and the second dissociation side surface, which are far away from the cutting edge, are respectively connected with the inner wall of the first accommodating cavity, the area of the first dissociation side surface is larger than that of the second dissociation side surface, and the flow groove is formed between the first dissociation side surface and the adjacent second dissociation side surface; or

The piece that dissociates include with the relative top surface that just the interval set up of terminal surface, and connect the top surface with first dissociation side, second dissociation side and cutting plane between the terminal surface, the cutting plane is connected first dissociation side with between the second dissociation side, first dissociation side is kept away from with the second dissociation side one side of cutting plane with the first interior wall connection who accepts the chamber, the area of first dissociation side is greater than the second dissociation side, first dissociation side and adjacent form between the second dissociation side the flowing groove.

7. A dissociation assembly as claimed in claim 4, wherein the cutter comprises a cutting portion located within the dissociation chamber and a guide portion extending from a side of the cutting portion remote from the rotational base to outside the dissociation chamber for guiding tissue into the dissociation chamber when the closure member is connected to the base, the guide portion covering a portion of the dissociation member in an orthographic projection of a radial plane of the dissociation chamber, the cutting portion including therein a radial portion extending radially from the connecting portion.

8. A dissociation assembly as claimed in claim 4, wherein the cutter comprises a cutting portion located in the dissociation chamber and a guide portion extending from a side of the cutting portion away from the rotary base to outside the dissociation chamber, the guide portion is used for guiding tissue into the dissociation chamber when the cover member is connected with the base, the guide portion covers a part of the dissociation member in a forward projection of a radial plane of the dissociation chamber, the cutting portion comprises a radial portion extending radially from the connecting body and a circumferential portion extending from one end of the radial portion away from the connecting body along a circumferential bending of the connecting body, and the circumferential portion is smoothly connected with the radial portion.

9. A dissociation assembly according to claim 7 or 8, wherein a single cutting portion is housed within the dissociation chamber, and the guide portion and the cutting portion do not overlap in orthographic projection of the radial plane; or at least two cutting parts are symmetrically arranged in the dissociation pool relative to the connecting body.

10. The dissociation assembly of claim 1,

one side of the rotating base body, which is adjacent to the connecting body, is provided with a fixing groove which is arranged in the circumferential direction;

the rotating piece further comprises a sealing body, and the sealing body is sleeved with and protrudes out of the fixing groove.

11. The dissociation assembly of claim 1,

the connector is equipped with cuts cut groove and a plurality of mobile hole, cut the groove along first connecting portion extremely the direction of second connecting portion extends, every mobile hole certainly connector periphery wall inwards extends and with cut the groove intercommunication.

12. A dissociation device, comprising:

the dissociation assembly of any one of claims 1 to 11, wherein the rotation base is provided with a rotation groove having an opening disposed away from the connection body, the rotation member further comprises a plurality of locking bodies, each locking body is connected to an inner wall of the rotation groove, and the plurality of locking bodies are disposed along a circumferential direction of the rotation groove;

13. A tissue dissociation tube, comprising:

14. A tissue dissociation method using the tissue dissociation tube of claim 13, comprising:

adding tissues into the accommodating tube, covering the base on the accommodating tube, and accommodating at least part of the tissues between the rotating piece and the dissociation piece;

15. A method of tissue dissociation employing the dissociation assembly of any one of claims 1-11, comprising: