CN115786075A

CN115786075A - Tissue dissociation device

Info

Publication number: CN115786075A
Application number: CN202211582712.XA
Authority: CN
Inventors: 李圆圆; 周颖婷; 吕洪臻; 汪楠
Original assignee: Jiangsu Vocational College of Medicine
Current assignee: Jiangsu Vocational College of Medicine
Priority date: 2022-12-09
Filing date: 2022-12-09
Publication date: 2023-03-14

Abstract

The invention discloses a tissue dissociation device, which relates to the technical field of tissue dissociation devices and comprises a sample bottle with an open top and a grinding and filtering mechanism arranged in the sample bottle, wherein the grinding and filtering mechanism comprises a grinding piece and a filtering assembly, the filtering assembly comprises a separating cylinder formed by surrounding a bottom plate and a surrounding plate, a tissue accommodating space for accommodating tissues and dissociation liquid is formed in the separating cylinder, and the outer wall of the surrounding plate is tightly attached to the inner wall of a cylindrical cavity and is in sliding sealing connection with the inner wall of the cylindrical cavity. The invention not only has double functions of grinding and filtering, but also realizes the sealing condition in the grinding process, has good sealing performance, can prevent dissociation liquid from permeating into a cell suspension accommodating area in the grinding process, thereby influencing the tissue dissociation efficiency and the cell suspension quality, and simultaneously, in the grinding process, the separation cylinder is driven by the axial driving assembly to vibrate, so that the tissue is fully contacted with the dissociation liquid, and the dissociation efficiency is improved.

Description

Tissue dissociation device

Technical Field

The invention relates to the technical field of tissue dissociation devices, in particular to a tissue dissociation device.

Background

The tissue dissociation device is equipment for dissociating biological tissues by utilizing a tissue dissociation technology to obtain high-activity and high-quality cell suspension. To obtain a cell suspension, the tissue dissociation device first needs to destroy the extracellular matrix to release cells from the extracellular matrix, and then needs to destroy the cell-to-cell connection to obtain the cell suspension.

There are many methods for tissue dissociation, such as enzymatic digestion, electrolysis, or grinding. Since the grinding can sufficiently shear the tissue, the dissociation effect is good, and the grinding is widely used. The existing device using grinding as a tissue dissociation method cannot directly filter dissociated cell suspension, and only can transfer the cell suspension into an external filtering device for filtering after grinding is finished, so that the operation is complicated, and the activity and the quality of the cell suspension can be influenced in the transfer process.

As in patent application CN114015543A, a tissue dissociation device is disclosed, comprising: a sample bottle having a storage space and a storage port; the grinding and filtering structure comprises a grinding piece and a filter screen, wherein the filter screen is movably connected to a grinding end of the grinding piece, the grinding end and the filter screen can enter the object placing space through the object placing opening, and the object placing space is divided into a cell suspension accommodating area positioned on one side of the filter screen and a tissue accommodating area (8230) \\8230), wherein the cell suspension accommodating area is positioned on one side of the grinding end, so that cell suspension formed by grinding biological tissues can pass through the filter screen under the action of negative pressure to be filtered and enter the cell suspension accommodating area. This tissue dissociation device will grind piece and filter screen integration together, and the integrated level is high, is favorable to improving the production efficiency and the activity of cell suspension, and filters cell suspension under the negative pressure effect, and the filter effect is better, does benefit to the cell suspension that forms high-quality. However, the patent also has the following disadvantages: it is sealed through grinding end and filter screen butt realization and seals, when grinding the end and taking place to rotate, probably take place to shake between grinding end and the filter screen and lead to appearing the clearance between the two, so, probably lead to when not dissociating the completion, the dissociation liquid can permeate the clearance between grinding end and the filter screen, gets into the cell suspension and holds the district to influence the efficiency and the cell suspension quality that the tissue dissociates.

Disclosure of Invention

The present invention is directed to a tissue dissociation device to solve the above problems.

In order to achieve the purpose, the invention adopts the following technical scheme:

the invention provides a tissue dissociation device, which comprises a sample bottle with an open top and a grinding and filtering mechanism arranged in the sample bottle, wherein the grinding and filtering mechanism comprises a grinding piece and a filtering component, the filtering component is movably connected with a grinding end of the grinding piece, the grinding end and the filtering component can extend into the sample bottle through the opening of the sample bottle and divide the interior of the sample bottle into a cell suspension containing area and a tissue containing area from bottom to top;

the filtering component comprises a separating cylinder formed by surrounding a bottom plate and a surrounding plate, a tissue accommodating space for accommodating tissues and dissociation liquid is formed inside the separating cylinder, the outer wall of the surrounding plate is tightly attached to the inner wall of the cylindrical cavity and is in sliding sealing connection with the inner wall of the cylindrical cavity, an annular isolation cavity is formed in the surrounding plate in a hollow mode, an annular partition plate is arranged in the middle of the bottom end of the annular isolation cavity along the height direction of the annular isolation cavity, the annular isolation cavity is divided by the annular partition plate to form an outer ring cavity and an inner ring cavity, a filtering structure for filtering cell suspension liquid is arranged at the bottom between the inner ring cavity and the tissue accommodating space, and through holes are uniformly formed in the bottom of the outer ring cavity along the circumferential direction of the outer ring cavity;

the septum of the filtration assembly has a mounting location inside the sample vial;

the grinding end is configured to be rotatable within the sample vial about a central axis thereof when the septum housing of the filter assembly is in the installed position to grind biological tissue located within the tissue-receiving space, and is further provided with an axial drive assembly configured to drive the septum housing to reciprocate axially in the installed position when the grinding end is rotated.

Furthermore, the top of the annular partition plate is connected with a conical cover, and the inner diameter of the small opening end of the conical cover is larger than the minimum inner diameter of the inner annular cavity.

Further, the spiral plates are uniformly arranged in the inner ring cavity along the circumferential direction, and the uniformly arranged spiral plates divide the inner ring cavity to form a plurality of spiral channels.

Further, the grinding end comprises a fixing shaft coaxially fixed at the bottom end of the tissue accommodating space, a grinding stator matched with the tissue accommodating space and fixedly assembled at the bottom end, a grinding rotor rotatably assembled at the outer side of the fixing shaft and matched with the grinding stator, and a driving part for driving the grinding rotor to rotate around the central axis of the fixing shaft so as to grind the biological tissue of the tissue accommodating space.

Furthermore, the grinding stator is in a circular truncated cone shape, a through hole matched with the fixed shaft is formed in the center of the grinding stator, a plurality of grinding grooves are formed in the conical surface of the grinding stator, and the grinding grooves rotate around the central axis of the fixed shaft;

the grinding rotor comprises a rotating ring rotatably arranged on the outer side of the fixed shaft, fixed strips symmetrically arranged on two sides of the fixed shaft and grinding blades connected to the bottoms of the fixed strips, and the bottom ends of the grinding blades are matched with the conical surface of the grinding stator and are provided with grinding blades;

the drive part includes the drive shaft, the drive shaft sets up with the fixed axle is coaxial, just the bottom and the fixed axle axial sliding connection of drive shaft, the top of drive shaft is extended and is connected with external driving source, the top of fixed strip is provided with T type post perpendicularly, and the outside cover of T type post is equipped with solid fixed ring, and is connected with the connecting rod between solid fixed ring and the drive shaft.

Furthermore, a groove is arranged at the top of the fixed shaft, the driving shaft extends into the groove and is axially and slidably connected with the groove, the axial driving assembly comprises a limiting structure arranged between the sample bottle and the separating cylinder and a clamping structure arranged in the groove,

the limiting structure comprises limiting strips uniformly arranged on the inner wall of the sample bottle along the circumferential direction, limiting grooves matched with the limiting strips are formed in the outer side wall of the bottom of the separating cylinder, the length of each limiting groove is greater than that of each limiting strip, and the separating cylinder is located at the installation position when the limiting grooves of the separating cylinder are clamped in the limiting strips;

the clamping structure comprises a non-radial sliding groove arranged on the outer side wall of the bottom end of the driving shaft and clamping blocks symmetrically fixed on two sides of the groove and connected with the non-radial sliding groove in a sliding mode, and when the driving shaft rotates for one circle, the clamping blocks undulate in the non-radial sliding groove at least once.

Further, the non-radial sliding groove comprises two opposite symmetrical wave crests and two opposite symmetrical wave troughs, and a smooth sliding channel is arranged between every two adjacent wave crests and wave troughs.

Further, the filtration includes that set up at the annular groove that is close to tissue accommodation space one side, set up along the circumferencial direction and be close to the punching a hole of inner ring chamber one side and install the filter screen in the annular groove, it is unanimous with the conical surface inclination of grinding the stator to punch a hole.

Compared with the prior art, the above one or more technical schemes have the following beneficial effects:

the invention not only has double functions of grinding and filtering, can filter the cell suspension in the fastest time after grinding is finished, and is beneficial to improving the production efficiency and activity of the cell suspension, but also realizes the sealing condition in the grinding process, has good sealing performance, can prevent dissociation liquid from permeating into a cell suspension accommodating area in the grinding process, thereby influencing the tissue dissociation efficiency and the cell suspension quality, simultaneously can not only lead the dissociation liquid and biological tissues of a tissue accommodating space to generate vibration under the driving of an axial driving assembly in the grinding process, is beneficial to the full contact of the dissociation liquid and tissue organisms, improves the dissociation efficiency, but also can intermittently generate jet flow in the tissue accommodating space, floats the fine tissues after grinding the bottom of the tissue accommodating space to be fully contacted with the dissociation liquid, and further improves the dissociation efficiency.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention, as claimed.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic cross-sectional structural view of the present invention;

FIG. 3 is a schematic view of a filter assembly and abrasive end configuration of the present invention;

FIG. 4 is a schematic view of the structure of the portion A of FIG. 3;

FIG. 5 is a schematic view of the structure of the part B in FIG. 3;

FIG. 6 is a schematic view of the filter assembly and abrasive article construction of the present invention;

FIG. 7 is a schematic illustration of a filter assembly and abrasive article of the present invention in a disassembled configuration;

FIG. 8 is a schematic view of a non-radial runner configuration of the present invention;

FIG. 9 is a schematic view of the axial upward movement of the spacer of the present invention;

FIG. 10 is a schematic view of the axially downward movement of the spacer of the present invention.

In the figure:

100. a sample bottle;

200. a grinding member; 210. grinding the end; 211. a fixed shaft; 212. grinding the stator; 213. grinding the rotor; 214. a drive member; 213a, a rotating ring; 213b, a fixing strip; 213c, a sharpening sheet; 214a, a drive shaft; 214b, T-shaped posts; 214c, a retaining ring; 214d, a connecting rod; 211a, a groove; 220. a driving end;

300. a filter assembly; 310. separating the cylinder; 311. a base plate; 312. enclosing plates; 320. a tissue receiving space; 330. an annular isolation chamber; 331. an outer ring cavity; 332. an inner ring cavity; 333. an annular partition plate; 334. a conical cover; 335. a spiral plate; 336. a helical channel; 340. a filter structure; 341. an annular groove; 342. punching; 343. filtering with a screen; 350. a through hole;

400. a cell suspension containment zone;

500. a tissue receiving area;

620. a clamping structure; 611. a limiting strip; 612. a limiting groove; 621. a non-radial chute; 622. a clamping block; 621a, peak; 621b, trough; 621c, a slide.

700. A cover body; 710. reserving a hole;

Detailed Description

In order to make the technical solutions better understood by those skilled in the art, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only partial embodiments of the present application, but not all embodiments. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present application without making any creative effort shall fall within the protection scope of the present application.

Referring to fig. 1-9, the present invention provides a tissue dissociation device, comprising a sample bottle 100 with an open top and a grinding and filtering mechanism disposed inside the sample bottle 100, wherein the grinding and filtering mechanism comprises a grinding member 200 and a filtering component 300, the grinding member 200 has a grinding end 210 and a driving end 220, the driving end 220 is used for connecting an external driving source (not shown), the filtering component 300 is movably connected to the grinding end 210 of the grinding member 200, the grinding end 210 and the filtering component 300 can extend into the sample bottle 100 through the open top of the sample bottle 100 and divide the interior of the sample bottle 100 into a cell suspension containing area 400 and a tissue containing area 500 from the bottom to the top.

As shown in fig. 2 and 3, the filter assembly 300 includes a partition cylinder 310 surrounded by a bottom plate 311 and a surrounding plate 312, a tissue accommodating space 320 for accommodating tissue and dissociation liquid is formed inside the partition cylinder 310, an outer wall of the surrounding plate 312 is tightly attached to an inner wall of the sample bottle 100 and is in sliding sealing connection with the inner wall of the sample bottle 100, the sliding sealing connection can be achieved by a sealing assembly in the prior art, for example, a sealing gasket is provided at an outer side of the partition cylinder and is tightly attached to the inner wall of the sample bottle 100, so as to achieve the sliding sealing connection, an annular isolation cavity 330 is hollow inside the surrounding plate 312, an annular partition plate 333 is provided at a middle portion of a bottom end of the annular isolation cavity 330 along a height direction thereof, the annular partition plate 333 partitions the annular isolation cavity 330 into an outer annular cavity 331 and an inner annular cavity 332, a filter structure 340 for filtering cell suspension is provided at a bottom portion between the inner cavity 332 and the tissue accommodating space 320, and through holes 350 are uniformly provided at a bottom portion of the outer cavity 331 along a circumference direction thereof;

the septum 310 of the filter assembly 300 has a mounting position inside the sample vial 100;

the abrading end 210 is configured to be rotatable about its central axis within the sample vial 100 when the septum 310 of the filter assembly 300 is in the installed position to abrade biological tissue located within the tissue-receiving space 320, the abrading end 210 further being provided with an axial drive assembly configured to drive the septum 310 to axially reciprocate in the installed position when the abrading end 210 is rotated.

The working principle is as follows: before application, the grinding and filtering mechanism is placed in the sample bottle 100 from the opening of the sample bottle 100, and the partition cylinder 310 of the filtering assembly 300 is moved to the installation position, and then the biological tissue and the dissociation liquid are poured into the sample bottle 100 from the opening of the sample bottle 100, and the biological tissue and the dissociation liquid enter the tissue accommodating space 320 of the partition cylinder 310 after entering the sample bottle 100, (it should be noted that the liquid level of the dissociation liquid is lower than the height of the annular partition plate, so the dissociation liquid is stored in the tissue accommodating space 320 and the inner annular cavity 332, and will not enter the cell suspension accommodating area, and at the same time, the cell suspension accommodating area forms a sealed environment due to the presence of the dissociation liquid), when in use, the driving end 220 of the grinding member 200 is externally connected, and the driving end can be a motor, and the motor drives the grinding member 200 to rotate, the grinding end 210 of the grinding member 200 grinds the biological tissue located in the tissue accommodating space 320, and at the same time, under the driving of the axial driving component, the partition cylinder 310 will generate reciprocating axial displacement at the installation position, when the partition cylinder 310 generates reciprocating axial displacement at the installation position, on one hand, the tissue accommodating space 320 will generate vibration to improve the dissociation efficiency, on the other hand, (please refer to fig. 9 and fig. 10) will compress the space of the cell suspension accommodating area intermittently, so that the gas in the cell suspension accommodating area enters the outer ring cavity 331 and the inner ring cavity 332 through the through hole 350, and further the dissociation liquid in the inner ring cavity 332 is discharged from the filtering structure 340 to form a jet flow, which can impact the ground fine tissue at the bottom of the tissue accommodating space 320, so that the fine tissue floats to fully contact with the dissociation liquid, further improving the dissociation efficiency, it should be noted that, during the reciprocating axial displacement of the partition cylinder 310 at the installation position, the space of the cell suspension accommodating area is compressed and then reset, and when the space is reset, a suction force is generated again, and the suction force can make the dissociation liquid in the tissue accommodating space 320 present a state of flowing to the filtering structure 340, and in this state, the floating biological tissue can be settled to the bottom of the tissue accommodating space 320, which is beneficial for the grinding of the biological tissue by the grinding end 210; after the grinding is completed, when the grinding and filtering mechanism is moved out, because the cell suspension accommodating area is in a sealed state, along with the continuous outward movement of the filtering component 300, the space of the cell suspension accommodating area is increased at the moment, and the air pressure is reduced, so that the cell suspension accommodating area forms a negative pressure state, and the cell suspension in the tissue accommodating space 320 enters the cell suspension accommodating area 400 through the inner annular cavity 332, the outer annular cavity 331 and the through hole 350 for temporary storage after being filtered by the filtering structure 340 under the action of negative pressure.

Compared with the prior art in the background art, the invention has the dual functions of grinding and filtering, can filter the cell suspension in the fastest time for finishing grinding, and is favorable for improving the production efficiency and activity of the cell suspension, the sealing condition in the grinding process of the invention is realized, the sealing property is good, the dissociation liquid can be prevented from permeating into the cell suspension accommodating area in the grinding process, so that the tissue dissociation efficiency and the cell suspension quality are influenced, and simultaneously, under the driving of the axial driving component in the grinding process, the dissociation liquid and the biological tissue in the tissue accommodating space 320 can vibrate, the dissociation liquid can be favorably and fully contacted with tissue organisms, the dissociation efficiency is improved, jet flow can be intermittently generated in the tissue accommodating space 320, and the ground fine tissue at the bottom of the tissue accommodating space 320 can float and fully contact with the dissociation liquid, so that the dissociation efficiency is further improved.

In this embodiment, as shown in fig. 1, the sample bottle 100 is in the shape of a test tube, and the sample bottle 100 has a cylindrical shape, and the sample bottle 100 can be directly placed on a centrifuge to perform centrifugation of the finally obtained cell suspension, and more specifically, the sample bottle 100 includes a straight cylinder portion and a tapered portion, and the inclined inner wall surface of the tapered portion enables the cell suspension to be easily collected to the bottom end of the sample bottle 100, thereby avoiding the occurrence of wall hanging.

In this embodiment, as shown in fig. 2, a conical cover 334 is connected to the top of the annular partition 333, and the inner diameter of the small opening end of the conical cover 334 is greater than the minimum inner diameter of the inner annular cavity 332. The conical cover 334 prevents the dissociation liquid in the inner ring chamber 332 from crossing the annular partition 333 and entering the outer ring chamber 331 during the up-and-down reciprocating movement of the partition cylinder 310.

In this embodiment, as shown in fig. 3 and 4, the spiral plates 335 are uniformly arranged inside the inner annular cavity 332 along the circumferential direction, and the uniformly arranged spiral plates 335 divide the inner annular cavity 332 into a plurality of spiral channels. In the process of continuously introducing the dissociation liquid into the inner annular cavity 332 and discharging the dissociation liquid out of the inner annular cavity 332, the liquids inside the inner annular cavity 332 are mixed by swirling flow, so as to balance the concentration of the dissociation liquid, i.e. the cell suspension, in the inner annular cavity 332 and the tissue accommodating space 320.

In the present embodiment, as shown in fig. 3 and 7, the grinding tip 210 includes a fixing shaft 211 coaxially fixed to the bottom end of the tissue accommodating space 320, a grinding stator 212 fitted to the tissue accommodating space 320 and fixedly fitted to the bottom end of the fixing shaft, a grinding rotor 213 rotatably fitted to the outside of the fixing shaft 211 and engaged with the grinding stator 212, and a driving member 214 for driving the grinding rotor 213 to rotate around the central axis of the fixing shaft 211 to grind the biological tissue of the tissue accommodating space 320. The grinding rotor 213 grinds the biological tissue inside the tissue accommodating space 320 by being continuously rotated in cooperation with the grinding stator 212.

Specifically, the grinding stator 212 is in a circular truncated cone shape, a through hole matched with the fixed shaft 211 is formed in the center of the grinding stator 212, a plurality of grinding grooves are formed in the conical surface of the grinding stator 212, and the grinding grooves rotate around the central axis of the fixed shaft 211; the conical surface of the grinding stator 212 is a grinding surface, and the grinding of the biological tissue is realized through the matching of the grinding grooves and the grinding rotor 213.

The grinding rotor 213 comprises a rotating ring 213a rotatably arranged at the outer side of the fixed shaft 211, fixed strips 213b symmetrically arranged at two sides of the fixed shaft, and grinding blades 213c connected to the bottom of the fixed strips 213b, wherein the bottom ends of the grinding blades 213c are matched with the conical surface of the grinding stator 212 and are provided with grinding blades; the position of the grinding blade 213c is relatively stationary in the axial direction of the grinding stator 212 by the position limitation of the rotating ring 213a, so that when the rotating ring 213a rotates, the grinding blade edge of the grinding blade 213c is matched with the grinding groove on the grinding surface to grind the biological tissue, and the biological tissue is sufficiently ground.

As shown in fig. 5 and 7, the driving part 214 includes a driving shaft 214a, the driving shaft 214a is disposed coaxially with the fixed shaft 211, a bottom end of the driving shaft 214a is axially slidably connected with the fixed shaft 211, a top end of the driving shaft 214a extends to be connected with an external driving source, a top portion of the fixing strip 213b is vertically provided with a T-shaped column 214b, a fixing ring 214c is sleeved outside the T-shaped column 214b, and a connecting rod 214d is connected between the fixing ring 214c and the driving shaft 214 a. The fixed ring 214c is driven by the driving shaft 214a to rotate circumferentially, and the T-shaped post 214b can drive the rotating ring 213a to rotate when the fixed ring 214c rotates circumferentially, because the partition cylinder 310 is driven by the axial driving assembly to reciprocate up and down axially, when the fixed ring 214c drives the T-shaped post 214b to rotate, the fixed ring 214c can slide on the T-shaped post 214b, so as to realize the axial movement of the rotating ring 213a during the circumferential rotation, that is, the rotating ring 213a can be driven by the driving shaft 214a to rotate circumferentially and satisfy the requirement of displacement in the axial direction, and is relatively static in the axial direction relative to the grinding stator 212.

Furthermore, in this embodiment, the top of the fixed shaft 211 is provided with a groove 211a, the driving shaft 214a extends into the groove 211a and is axially slidably connected with the groove 211a, and the axial driving assembly includes a limiting structure disposed between the sample bottle 100 and the separating cylinder 310 and a clamping structure disposed in the groove 211 a;

as shown in fig. 3 and 7, the limiting structure includes a limiting strip 611 uniformly arranged on the inner wall of the sample bottle 100 along the circumferential direction, a limiting groove 612 adapted to the limiting strip 611 is arranged on the outer side wall of the bottom of the partition cylinder 310, the length of the limiting groove 612 is greater than the length of the limiting strip 611, and when the limiting groove 612 of the partition cylinder 310 is clamped in the limiting strip 611, the partition cylinder 310 is located at the installation position;

as shown in fig. 5 and 7, the locking structure includes a non-radial sliding slot 621 disposed on the outer side wall of the bottom end of the driving shaft 214a, and a latch 622 symmetrically fixed on both sides of the recess 211a and slidably connected with the non-radial sliding slot 621, wherein the latch 622 undulates at least once in the non-radial sliding slot 621 when the driving shaft 214a rotates one revolution.

Through the arrangement of the clamping structure, when the partition cylinder 310 is located at the installation position, the circumferential rotation is limited, and thus, through the arrangement of the clamping structure, when the driving shaft 214a rotates, the fixture block 622 is clamped in the non-radial sliding groove 621, so that the fixing column can fluctuate at least once when the driving shaft 214a rotates a circle, that is, the partition cylinder 310 is located at the installation position, and along with the circumferential rotation of the driving shaft 214a, the partition cylinder 310 continuously generates axial displacement, and the aforementioned effects are generated.

Specifically, as shown in fig. 8, in the present embodiment, the non-radial sliding groove 621 includes two symmetrical peaks 621a and two symmetrical troughs 621b, and a smooth sliding path 621c is disposed between adjacent peaks 621a and troughs 621 b.

Further, as shown in fig. 4, in the present embodiment, the filtering structure 340 includes an annular recess 341 opened at a side close to the tissue accommodating space 320, punched holes 342 circumferentially provided at a side close to the inner annular chamber 332, and a screen 343 installed in the annular recess 341, the punched holes 342 corresponding to the taper of the conical surface of the grinding stator 212. Through the design, along with the reciprocating movement of the separating cylinder 310 in the axial direction, the gas in the cell suspension accommodating area 400 can push the dissociation liquid in the inner ring cavity 332 to be sprayed out from the punching hole 342 along the grinding surface of the grinding stator 212 after being extruded, so that the ground fine tissue can be floated to be fully contacted with the dissociation liquid, and the dissociation efficiency is improved.

In this embodiment, as shown in fig. 1, a cover 700 is fastened to the top of the sampling bottle, and a preformed hole 710 for the driving shaft 214a to pass through is reserved in the middle of the cover.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered as the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.

Claims

1. A tissue dissociation device comprises a sample bottle with an open top and a grinding and filtering mechanism arranged inside the sample bottle, wherein the grinding and filtering mechanism comprises a grinding piece and a filtering component, the filtering component is movably connected to a grinding end of the grinding piece, and the grinding end and the filtering component can extend into the sample bottle through the opening of the sample bottle and divide the inside of the sample bottle into a cell suspension containing area and a tissue containing area from bottom to top;

it is characterized in that the preparation method is characterized in that,

2. The tissue dissociation device of claim 1, wherein a conical hood is attached to a top portion of the annular spacer, and an inner diameter of a small opening end of the conical hood is greater than a minimum inner diameter of the inner annular cavity.

3. The tissue dissociation device of claim 1, wherein the inner annular chamber is uniformly circumferentially provided with helical plates, and the uniformly provided helical plates divide the inner annular chamber into a plurality of helical channels.

4. The tissue dissociation device of claim 1, wherein the grinding end comprises a fixing shaft coaxially fixed to the bottom end of the tissue accommodating space, a grinding stator fitted to the tissue accommodating space and fixedly mounted to the bottom end, a grinding rotor rotatably mounted to an outer side of the fixing shaft and cooperating with the grinding stator, and a driving member for driving the grinding rotor to rotate around a central axis of the fixing shaft to grind the biological tissue of the tissue accommodating space.

5. The tissue dissociation device according to claim 4, wherein the grinding stator is in a circular truncated cone shape, a through hole adapted to the fixed shaft is formed in the center of the grinding stator, a plurality of grinding grooves are formed in a conical surface of the grinding stator, and the plurality of grinding grooves rotate around a central axis of the fixed shaft;

6. The tissue dissociation device of claim 5, wherein a groove is disposed at the top of the fixed shaft, the driving shaft extends into the groove and is axially slidably connected with the groove, the axial driving assembly comprises a limiting structure disposed between the sample bottle and the separating cylinder and a clamping structure disposed in the groove,

the limiting structure comprises limiting strips uniformly arranged on the inner wall of the sample bottle along the circumferential direction, the outer side wall of the bottom of the partition cylinder is provided with a limiting groove matched with the limiting strips, the length of the limiting groove is greater than that of the limiting strips, and when the limiting groove of the partition cylinder is clamped in the limiting strips, the partition cylinder is positioned at the mounting position;

the clamping structure comprises a non-radial sliding groove arranged on the outer side wall of the bottom end of the driving shaft and clamping blocks symmetrically fixed on two sides of the groove and in sliding connection with the non-radial sliding groove, and when the driving shaft rotates for one circle, the clamping blocks fluctuate in the non-radial sliding groove at least once.

7. A tissue dissociation device as claimed in claim 6, wherein the non-radial runners comprise two peaks and two troughs which are relatively symmetrical, a smooth ramp being provided between adjacent peaks and troughs.

8. The tissue dissociation device of claim 5, wherein the filtering structure comprises an annular groove opened at a side close to the tissue accommodating space, a punched hole circumferentially disposed at a side close to the inner annular cavity, and a strainer installed in the annular groove, the punched hole having a slope corresponding to a conical surface of the grinding stator.