CN114854590A - Stem cell filtering separator - Google Patents
Stem cell filtering separator Download PDFInfo
- Publication number
- CN114854590A CN114854590A CN202210675546.1A CN202210675546A CN114854590A CN 114854590 A CN114854590 A CN 114854590A CN 202210675546 A CN202210675546 A CN 202210675546A CN 114854590 A CN114854590 A CN 114854590A
- Authority
- CN
- China
- Prior art keywords
- hole
- shaped
- cylinder
- filter membrane
- stem cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B9/00—Drives specially designed for centrifuges; Arrangement or disposition of transmission gearing; Suspending or balancing rotary bowls
- B04B9/02—Electric motor drives
- B04B9/04—Direct drive
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B9/00—Drives specially designed for centrifuges; Arrangement or disposition of transmission gearing; Suspending or balancing rotary bowls
- B04B9/08—Arrangement or disposition of transmission gearing ; Couplings; Brakes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/05—Means for pre-treatment of biological substances by centrifugation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Centrifugal Separators (AREA)
Abstract
The invention discloses a stem cell filtering separator, which relates to the technical field of biological equipment and comprises a separating cylinder, wherein a cylinder cover is arranged at the top of the separating cylinder, a centrifugal motor is arranged at the center of the top of the cylinder cover, the top of the centrifugal motor is connected with the side part of the separating cylinder through a lifting mechanism, and a rotary hole is formed in the top of the cylinder cover. The centrifugal separation device is reasonable in structure, when centrifugal separation is needed, the centrifugal motor is started, the output shaft of the centrifugal motor rotates to drive the centrifugal motor, the connecting shaft and the fixed shaft to rotate, the fixed shaft rotates to drive the movable block and the inner ring to rotate, meanwhile, the fixed shaft rotates to drive the cup-shaped filter membrane frame to rotate, the filter membrane on the cup-shaped filter membrane frame rotates to carry out centrifugal separation on stem cells, and when the stem cells fall down from the through holes, the stem cells can move upwards at the inclined circumferential side part of the cup-shaped filter membrane frame due to centrifugal force and are in contact with the inner wall of the retaining sleeve, so that separation is carried out.
Description
Technical Field
The invention relates to the technical field of biological equipment, in particular to a stem cell filtering separator.
Background
At present, stem cells refer to primitive cells with self-renewal and multi-directional differentiation potential, which are originated cells of the body and can be differentiated into one or more cells constituting tissues or organs of the body under specific conditions, and the stem cells need to be filtered and separated during cultivation.
Current centrifugal separation device is when separating, and stem cell can be put into the back and can be rotated by centrifugal force's influence, and the unable circulation is centrifuged to the separation effect is poor, for this reason, this application discloses a stem cell filter separator satisfies people's demand.
Disclosure of Invention
The present application is directed to a stem cell filtering separator, which solves the above problems of the prior art.
In order to achieve the above purpose, the present application provides the following technical solutions: the utility model provides a stem cell filters separator, includes the cylinder, the cover is installed at the top of cylinder, the top central point of cover puts and installs centrifugal motor, centrifugal motor's top pass through hoist mechanism with the lateral part of cylinder is connected, the top of cover has been seted up and has been changeed the hole, change the hole with the inside of cylinder is linked together, centrifugal motor output shaft has the connecting axle, the bottom of connecting axle is run through change the hole and install the pinion, the fixed axle is installed to the bottom of pinion, cup-shaped filter membrane frame is installed to the bottom of fixed axle, be provided with the filter membrane in the cup-shaped filter membrane frame, the spacer sleeve is installed at the top of cup-shaped filter membrane frame, the spacer sleeve with the inner wall cooperation installation of cylinder, install backward flow mechanism on the fixed axle.
Preferably, the backflow mechanism comprises a movable hole arranged on the side part of the fixed shaft, a movable block is slidably arranged in the movable hole, inner rings are arranged at two ends of the movable block, a bearing is arranged on the side part of the circumference of the inner ring, the side part of the bearing is connected with a pressing unit, two inclined rods are arranged at the bottom of the bearing, the inner wall of the inner ring of the bearing is connected with the side part of the circumference of the inner ring, the top ends of the two inclined rods are connected to the bottom of the outer ring of the bearing, a conical disc is arranged at the bottom ends of the two inclined rods, a gap is reserved between the bottom of the conical disc and the top of the fixed shaft, an outer ring is arranged on the side part of a circular shaft of the conical disc, an inclined plate is arranged at the top of the outer ring, the inclined plate is obliquely arranged, the inclined plate is in contact with the inner wall of the blocking sleeve, the bottom of the outer ring is obliquely arranged, and the outer ring is matched with the top of the oblique circumference of the cup-shaped filter membrane frame, a through hole is formed in the top of the conical disc, one end of the fixing shaft penetrates through the through hole, and a gap is reserved between the inner wall of the through hole and the side portion of the fixing shaft.
Preferably, the unit of pushing down install in including rotating the rotation axis of cover bottom, the bottom of rotation axis is rotated and is installed the gear wheel, the diameter of gear wheel is greater than the diameter of pinion, the gear wheel with the pinion meshes mutually, the down tube is installed to the bottom off-centre of gear wheel, L shape mounting panel is installed to the bottom of cover, the rectangular hole has been seted up at the top of L shape mounting panel, slidable mounting has the rectangle post in the rectangular hole, isosceles triangle is installed on the top of rectangle post, isosceles triangle with the position of down tube is corresponding, the spout has been seted up to the bottom of rectangle post, slidable mounting has the cylinder in the spout, the connecting plate is installed to the lateral part of bearing, the slide opening has been seted up at the top of connecting plate, cylindrical bottom runs through the slide opening is connected with the closure tray.
Preferably, the rectangular column is sleeved with an upper spring, and the top end and the bottom end of the upper spring are respectively installed on the side portions, close to each other, of the isosceles triangular block and the L-shaped installation plate.
Preferably, the cylinder is sleeved with a lower spring, and two ends of the lower spring are respectively installed on the connecting plate and the side part of the rectangular column, which are close to each other.
Preferably, the lifting mechanism comprises an L-shaped lifting rod mounted at the top of the centrifugal motor, an L-shaped movable plate is mounted at the side of the separation cylinder, a rack is mounted at the side of the L-shaped movable plate, a lifting motor is mounted at the side of the L-shaped lifting rod, a rotating gear is mounted at an output shaft of the lifting motor, the rotating gear is meshed with the rack, and the L-shaped lifting rod is slidably connected with the L-shaped movable plate.
Preferably, a guide block is mounted on the side portion of the L-shaped movable plate, a guide hole is formed in the top of the guide block, and one end of the L-shaped lifting rod is slidably mounted in the guide hole.
Preferably, the top of cover is installed the inlet pipe, the discharging pipe is installed to the bottom of knockout drum.
In conclusion, the technical effects and advantages of the invention are as follows:
1. the centrifugal separation device is reasonable in structure, when centrifugal separation is needed, the centrifugal motor is started, the output shaft of the centrifugal motor rotates to drive the centrifugal motor, the connecting shaft and the fixed shaft to rotate, the fixed shaft rotates to drive the movable block and the inner ring to rotate, meanwhile, the fixed shaft rotates to drive the cup-shaped filter membrane frame to rotate, the filter membrane on the cup-shaped filter membrane frame rotates to carry out centrifugal separation on stem cells, and when the stem cells fall down from the through holes, the stem cells can move upwards at the inclined circumferential side part of the cup-shaped filter membrane frame due to centrifugal force and are in contact with the inner wall of the retaining sleeve, so that separation is carried out;
2. in the invention, a pinion rotates to drive a gearwheel to rotate, the gearwheel rotates to drive an inclined rod to rotate, when the inclined rod is contacted with an isosceles triangle block, the inclined rod extrudes the isosceles triangle block to enable the isosceles triangle block to move downwards, the isosceles triangle block moves to drive an upper spring to deform, simultaneously, the isosceles triangle block moves to drive a rectangular column to move, the rectangular column moves to extrude a lower spring to enable the rectangular column to deform, a connecting plate moves downwards under the reaction force of the lower spring, the connecting plate moves to drive a bearing and two inclined rods to move downwards, further, a conical disc and an outer ring move downwards, the outer ring is contacted with the top of a fixed shaft, so that stem cells above the outer ring on a cup-shaped filter membrane frame cannot fall onto the cup-shaped filter membrane frame, when the stem cells are contacted with the inclined plate, the stem cells are stopped by the inclined plate to fall onto the conical disc, and further fall onto the fixed shaft through a through hole, and continuing to circulate and centrifuge.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative efforts.
Fig. 1 is a schematic perspective view of a first perspective structure according to an embodiment of the present application;
FIG. 2 is an enlarged schematic view of the structure at A in FIG. 1;
FIG. 3 is a schematic view of a cut-away perspective structure according to an embodiment of the present application;
FIG. 4 is a schematic view of a portion of the structure of FIG. 3;
FIG. 5 is a schematic view of a portion of the structure of FIG. 4;
fig. 6 is a schematic perspective view of a second perspective structure according to an embodiment of the present application.
In the figure: 1. a separation cylinder; 2. a cylinder cover; 3. a centrifugal motor; 4. a connecting shaft; 5. a pinion gear; 6. a fixed shaft; 7. a cup-shaped filter membrane frame; 8. a blocking sleeve; 9. a movable hole; 10. a movable block; 11. an inner ring; 12. a bearing; 13. a connecting plate; 14. an L-shaped mounting plate; 15. a rectangular column; 16. an isosceles triangle block; 17. an upper spring; 18. a cylinder; 19. a lower spring; 20. blocking the disc; 21. a rotating shaft; 22. a bull gear; 23. a diagonal bar; 24. a conical disk; 25. an outer ring; 26. a through hole; 27. an L-shaped lifting rod; 28. an L-shaped movable plate; 29. a guide block; 30. a rack; 31. a lifting motor; 32. a rotating gear; 33. a sloping plate.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example one
Referring to fig. 1-6, a stem cell filter separator, including a separation cylinder 1, a cylinder cover 2 is installed at the top of the separation cylinder 1, a centrifugal motor 3 is installed at the top center position of the cylinder cover 2, the top of the centrifugal motor 3 is connected with the side portion of the separation cylinder 1 through a lifting mechanism, a rotary hole is formed at the top of the cylinder cover 2 and communicated with the inside of the separation cylinder 1, an output shaft of the centrifugal motor 3 is connected with a connecting shaft 4, the bottom end of the connecting shaft 4 penetrates through the rotary hole and is provided with a pinion 5, a fixing shaft 6 is installed at the bottom end of the pinion 5, a cup-shaped filter membrane frame 7 is installed at the bottom end of the fixing shaft 6, a filter membrane is arranged in the cup-shaped filter membrane frame 7, a baffle sleeve 8 is installed at the top of the cup-shaped filter membrane frame 7, the baffle sleeve 8 is installed in cooperation with the inner wall of the separation cylinder 1, and a backflow mechanism is installed on the fixing shaft 6. When centrifugal separation is needed, the centrifugal motor 3 is started, the output shaft of the centrifugal motor 3 rotates to drive the centrifugal motor 3, the connecting shaft 4 and the fixing shaft 6 to rotate, the fixing shaft 6 rotates to drive the movable block 10 and the inner ring 11 to rotate, meanwhile, the fixing shaft 6 rotates to drive the cup-shaped filter membrane frame 7 to rotate, the filter membrane on the cup-shaped filter membrane frame 7 rotates to carry out centrifugal separation on stem cells, and when the stem cells fall from the through hole 26, the stem cells can move upwards at the inclined circumferential side part of the cup-shaped filter membrane frame 7 due to centrifugal force and are in contact with the inner wall of the blocking sleeve 8, so that separation is carried out.
Example two
Based on the above embodiment 1, the backflow mechanism includes the movable hole 9 provided at the side of the fixed shaft 6, the movable block 10 is slidably installed in the movable hole 9, the inner rings 11 are installed at both ends of the movable block 10, the bearing 12 is installed at the circumferential side of the inner ring 11, the pressing unit is connected to the side of the bearing 12, the two inclined rods 23 are installed at the bottom of the bearing 12, the inner wall of the inner ring of the bearing 12 is connected to the circumferential side of the inner ring 11, the top ends of the two inclined rods 23 are connected to the bottom of the outer ring of the bearing 12, the conical disc 24 is installed at the bottom of the two inclined rods 23, a gap is left between the bottom of the conical disc 24 and the top of the fixed shaft 6, the outer ring 25 is installed at the circumferential side of the conical disc 24, the inclined plate 33 is installed at the top of the outer ring 25, the inclined plate 33 is in contact with the inner wall of the retaining sleeve 8, the bottom of the outer ring 25 is in an inclined arrangement, the outer ring 25 is adapted to the inclined circumferential top of the cup-shaped filter frame 7, the top of the conical disc 24 is provided with a through hole 26, one end of the fixed shaft 6 penetrates through the through hole 26, and a gap is reserved between the inner wall of the through hole 26 and the side part of the fixed shaft 6.
EXAMPLE III
Based on above-mentioned embodiment 1 or 2, the unit of pushing down installs in the rotation axis 21 of cover 2 bottom including rotating, rotation axis 21's bottom is rotated and is installed gear wheel 22, gear wheel 22's diameter is greater than pinion 5's diameter, gear wheel 22 meshes with pinion 5 mutually, gear wheel 22's bottom eccentric mounting has down tube 23, L shape mounting panel 14 is installed to cover 2's bottom, the rectangular hole has been seted up at L shape mounting panel 14's top, slidable mounting has rectangle post 15 in the rectangular hole, isosceles triangle 16 is installed on rectangle post 15's top, isosceles triangle 16 corresponds with the position of down tube 23, the spout has been seted up to rectangle post 15's bottom, slidable mounting has cylinder 18 in the spout, connecting plate 13 is installed to the lateral part of bearing 12, the slide opening has been seted up at the top of connecting plate 13, the bottom of cylinder 18 runs through the slide opening and is connected with closure disc 20. Pinion 5 rotates and drives gear wheel 22 to rotate, and gear wheel 22 rotates and drives down tube 23 to rotate, and when down tube 23 and isosceles triangle piece 16 contact, down tube 23 extrudes isosceles triangle piece 16 and makes it move down, and isosceles triangle piece 16 removes and drives upper spring 17 and take place deformation, and isosceles triangle piece 16 removes and drives rectangle post 15 and remove simultaneously.
Example four
Based on the above embodiments 1, 2 or 3, the rectangular column 15 is sleeved with the upper spring 17, and the top end and the bottom end of the upper spring 17 are respectively installed on the side portions of the isosceles triangle block 16 and the L-shaped installation plate 14, which are close to each other.
EXAMPLE five
Based on the above embodiments 1, 2, 3 or 4, the lower spring 19 is sleeved on the column 18, and both ends of the lower spring 19 are respectively installed on the side portions of the connecting plate 13 and the rectangular column 15, which are close to each other. Rectangular column 15 moves and extrudes lower spring 19 to deform it, connecting plate 13 moves downwards under the reaction force of lower spring 19, connecting plate 13 moves to drive bearing 12 and two diagonal rods 23 to move downwards, and then conical disc 24 and outer ring 25 move downwards, outer ring 25 contacts with the top of fixed shaft 6, so that the stem cells above outer ring 25 on cup-shaped filter frame 7 do not fall onto cup-shaped filter frame 7, when the stem cells contact with inclined plate 33, the stem cells can fall onto conical disc 24 due to the blocking of inclined plate 33, and the stem cells can continuously fall onto fixed shaft 6 through hole 26, and the circulation centrifugation is continued.
EXAMPLE six
Based on the above embodiments 1, 2, 3, 4, or 5, the lifting mechanism includes an L-shaped lifting rod 27 mounted on the top of the centrifugal motor 3, an L-shaped movable plate 28 is mounted on the side of the separation cylinder 1, a rack 30 is mounted on the side of the L-shaped movable plate 28, a lifting motor 31 is mounted on the side of the L-shaped lifting rod 27, a rotating gear 32 is mounted on an output shaft of the lifting motor 31, the rotating gear 32 is engaged with the rack 30, and the L-shaped lifting rod 27 is slidably connected with the L-shaped movable plate 28. Can drive rotatory gear 32 through starting lifting motor 31 and rotate, rotatory gear 32 rotates on rack 30 to make L shape lifting rod 27 and cover 2 rebound, thereby be convenient for later maintenance.
EXAMPLE seven
Based on the above embodiments 1, 2, 3, 4, 5, or 6, the guide block 29 is installed at the side of the L-shaped movable plate 28, the top of the guide block 29 is opened with a guide hole, and one end of the L-shaped lifting rod 27 is slidably installed in the guide hole. The L-shaped lifting rod 27 is convenient to limit and guide through the arrangement of the guide block 29.
Example eight
Based on the above embodiments 1, 2, 3, 4, 5, 6 or 7, the top of the cover 2 is installed with the feeding pipe, and the bottom of the separation cylinder 1 is installed with the discharging pipe. Can put into stem cell through the inlet pipe, can discharge through the discharging pipe.
The working principle of the invention is as follows:
stem cells can be put in through the feeding pipe and can be discharged through the discharging pipe, when centrifugal separation is needed, the centrifugal motor 3 is started, the output shaft of the centrifugal motor 3 rotates to drive the centrifugal motor 3, the connecting shaft 4 and the fixed shaft 6 to rotate, the fixed shaft 6 rotates to drive the movable block 10 and the inner ring 11 to rotate, meanwhile, the fixed shaft 6 rotates to drive the cup-shaped filter membrane frame 7 to rotate, the filter membrane on the cup-shaped filter membrane frame 7 rotates to carry out centrifugal separation on the stem cells, and when the stem cells fall down from the through hole 26, the stem cells can move upwards at the inclined circumferential side part of the cup-shaped filter membrane frame 7 due to centrifugal force and are contacted with the inner wall of the retaining sleeve 8, so that separation is carried out;
meanwhile, the pinion 5 rotates to drive the bull gear 22 to rotate, the bull gear 22 rotates to drive the inclined rod 23 to rotate, when the inclined rod 23 is in contact with the isosceles triangle block 16, the inclined rod 23 presses the isosceles triangle block 16 to move downwards, the isosceles triangle block 16 moves to drive the upper spring 17 to deform, meanwhile, the isosceles triangle block 16 moves to drive the rectangular column 15 to move, the rectangular column 15 moves to press the lower spring 19 to deform, the connecting plate 13 moves downwards under the reaction force of the lower spring 19, the connecting plate 13 moves to drive the bearing 12 and the two inclined rods 23 to move downwards, further, the conical disc 24 and the outer ring 25 move downwards, the outer ring 25 is in contact with the top of the fixed shaft 6, accordingly, stem cells above the outer ring 25 on the cup-shaped filter film frame 7 cannot fall onto the cup-shaped filter film frame 7, when the stem cells are in contact with the inclined plate 33, the stem cells can fall onto the conical disc 24 due to blocking of the inclined plate 33, so that the stem cells continuously drop onto the fixed shaft 6 through the through holes 26, and the circulation centrifugation is continuously carried out;
after the centrifugation is accomplished, can drive rotatory gear 32 through starting lifting motor 31 and rotate, rotatory gear 32 rotates on rack 30 to make L shape lifting rod 27 and cover 2 rebound, thereby be convenient for later maintenance, simultaneously through the setting of guide block 29, be convenient for carry out spacing direction to L shape lifting rod 27.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (8)
1. A stem cell filtration separator, comprising: comprises a separating cylinder (1), a cylinder cover (2) is installed at the top of the separating cylinder (1), a centrifugal motor (3) is installed at the top center position of the cylinder cover (2), the top of the centrifugal motor (3) is connected with the side part of the separating cylinder (1) through a lifting mechanism, a rotary hole is formed in the top of the cylinder cover (2), the rotary hole is communicated with the inside of the separating cylinder (1), a connecting shaft (4) is connected with an output shaft of the centrifugal motor (3), the bottom end of the connecting shaft (4) penetrates through the rotary hole and is provided with a pinion (5), a fixed shaft (6) is installed at the bottom end of the pinion (5), a cup-shaped filter membrane frame (7) is installed at the bottom end of the fixed shaft (6), a filter membrane is arranged in the cup-shaped filter membrane frame (7), a baffle sleeve (8) is installed at the top of the cup-shaped filter membrane frame (7), and the baffle sleeve (8) is installed in cooperation with the inner wall of the separating cylinder (1), and a backflow mechanism is arranged on the fixed shaft (6).
2. The stem cell filtration separator of claim 1, wherein: the backflow mechanism comprises a movable hole (9) formed in the side portion of the fixed shaft (6), a movable block (10) is arranged in the movable hole (9) in a sliding mode, inner rings (11) are installed at two ends of the movable block (10), bearings (12) are installed on the side portion of the circumference of the inner rings (11), the side portion of each bearing (12) is connected with a pressing unit, two inclined rods (23) are installed at the bottom of each bearing (12), the inner ring inner wall of each bearing (12) is connected with the side portion of the circumference of the inner rings (11), the top ends of the two inclined rods (23) are connected to the bottom of the outer ring of each bearing (12), conical discs (24) are installed at the bottom ends of the inclined rods (23), gaps are reserved at the bottoms of the conical discs (24) and the top portion of the fixed shaft (6), outer rings (25) are installed on the side portions of the circular shafts of the conical discs (24), swash plate (33) are installed at the top of outer ring (25), swash plate (33) slope sets up, swash plate (33) with the inner wall of spacer sleeve (8) contacts, the bottom of outer ring (25) sets up for the slope, outer ring (25) with the slope circumference top looks adaptation of cup-shaped filter membrane frame (7), through-hole (26) have been seted up at the top of toper dish (24), the one end of fixed axle (6) is run through-hole (26), the inner wall of through-hole (26) with fixed axle (6) lateral part leaves the clearance.
3. A stem cell filtration separator according to claim 2, wherein: the unit of pushing down install in rotation axis (21) of cover (2) bottom including rotating, the bottom of rotation axis (21) is rotated and is installed gear wheel (22), the diameter of gear wheel (22) is greater than the diameter of pinion (5), gear wheel (22) with pinion (5) mesh mutually, down tube (23) are installed to the bottom off-centre of gear wheel (22), L shape mounting panel (14) are installed to the bottom of cover (2), the rectangular hole has been seted up at the top of L shape mounting panel (14), slidable mounting has rectangle post (15) in the rectangular hole, isosceles triangle piece (16) are installed on the top of rectangle post (15), isosceles triangle piece (16) with the position of down tube (23) is corresponding, the spout has been seted up to the bottom of rectangle post (15), slidable mounting has cylinder (18) in the spout, connecting plate (13) are installed to the lateral part of bearing (12), the slide opening has been seted up at the top of connecting plate (13), the bottom of cylinder (18) runs through the slide opening and be connected with closure disc (20).
4. A stem cell filtration separator according to claim 3, wherein: an upper spring (17) is sleeved on the rectangular column (15), and the top end and the bottom end of the upper spring (17) are respectively installed on the side portions, close to the isosceles triangle block (16) and the L-shaped installation plate (14), of the upper spring.
5. A stem cell filtration separator according to claim 3, wherein: the cylinder (18) is sleeved with a lower spring (19), and two ends of the lower spring (19) are respectively installed on the side portions, close to each other, of the connecting plate (13) and the rectangular column (15).
6. The stem cell filtration separator of claim 1, wherein: the lifting mechanism comprises an L-shaped lifting rod (27) arranged at the top of the centrifugal motor (3), an L-shaped movable plate (28) is arranged on the side portion of the separating cylinder (1), a rack (30) is arranged on the side portion of the L-shaped movable plate (28), a lifting motor (31) is arranged on the side portion of the L-shaped lifting rod (27), a rotating gear (32) is arranged on an output shaft of the lifting motor (31), the rotating gear (32) is meshed with the rack (30), and the L-shaped lifting rod (27) is connected with the L-shaped movable plate (28) in a sliding mode.
7. The stem cell filter separator of claim 6, wherein: a guide block (29) is installed on the side portion of the L-shaped movable plate (28), a guide hole is formed in the top of the guide block (29), and one end of the L-shaped lifting rod (27) is installed in the guide hole in a sliding mode.
8. The stem cell filtration separator of claim 1, wherein: the inlet pipe is installed at the top of cover (2), the discharging pipe is installed to the bottom of knockout drum (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210675546.1A CN114854590A (en) | 2022-06-15 | 2022-06-15 | Stem cell filtering separator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210675546.1A CN114854590A (en) | 2022-06-15 | 2022-06-15 | Stem cell filtering separator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114854590A true CN114854590A (en) | 2022-08-05 |
Family
ID=82624273
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210675546.1A Withdrawn CN114854590A (en) | 2022-06-15 | 2022-06-15 | Stem cell filtering separator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114854590A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117586859A (en) * | 2023-11-28 | 2024-02-23 | 上海多宁生物科技股份有限公司 | Stem cell conditioned medium purification device and application method thereof |
| CN118546756A (en) * | 2024-05-15 | 2024-08-27 | 上海昱极细胞生物技术有限公司 | Separation preparation device and method for natural killer cells |
-
2022
- 2022-06-15 CN CN202210675546.1A patent/CN114854590A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117586859A (en) * | 2023-11-28 | 2024-02-23 | 上海多宁生物科技股份有限公司 | Stem cell conditioned medium purification device and application method thereof |
| CN117586859B (en) * | 2023-11-28 | 2024-07-05 | 上海多宁生物科技股份有限公司 | Stem cell conditioned medium purification device and application method thereof |
| CN118546756A (en) * | 2024-05-15 | 2024-08-27 | 上海昱极细胞生物技术有限公司 | Separation preparation device and method for natural killer cells |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN114854590A (en) | Stem cell filtering separator | |
| CN114456891B (en) | Red bayberry wine brewing raw material processing device with filtering and screening functions | |
| CN214972253U (en) | Filter equipment is used in soybean oil processing | |
| CN219630792U (en) | Filter equipment for preparation methyl ethyl carbonate | |
| CN218653319U (en) | Centrifugal rainwater collecting and filtering device | |
| CN216963796U (en) | High-efficient solid-liquid separation equipment | |
| CN214763739U (en) | Filter press | |
| CN115786075A (en) | Tissue dissociation device | |
| CN110681486A (en) | Novel centrifugal separation equipment of magnetic material for industrial waste liquid | |
| CN216170590U (en) | Linseed oil adsorbs concise with scarfing cinder essence jar of straining | |
| CN214347250U (en) | Horizontal spiral discharging and filtering centrifuge | |
| CN209829287U (en) | Vertical low-speed large-capacity centrifuge suitable for test tubes with different diameters | |
| CN216125856U (en) | Cell collection device for medical treatment | |
| CN210905267U (en) | Solid-liquid separation device for producing surfactant through multi-stage separation | |
| CN220495783U (en) | Centrifugal filter | |
| CN114414459B (en) | Sample intelligent efficient preparation system for flow cytometry and application method thereof | |
| CN111410340A (en) | Sewage treatment device | |
| CN221107364U (en) | ARA fungus body preparation filter equipment | |
| CN213285955U (en) | Special blood separator | |
| CN116713126B (en) | Rolling extraction device for saussurea involucrata processing | |
| CN211536850U (en) | Filter equipment is used in edible oil production | |
| CN220657907U (en) | Centrifugal machine for producing tranexamic acid bulk drug | |
| CN220609431U (en) | Hydrogenation liquid catalyst separation device | |
| CN221158014U (en) | Exhaust gas collecting device for discharging of suction filtration bucket | |
| CN218130427U (en) | Filter equipment is used in honey processing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20220805 |