CN114414459B - Sample intelligent efficient preparation system for flow cytometry and application method thereof - Google Patents
Sample intelligent efficient preparation system for flow cytometry and application method thereof Download PDFInfo
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- CN114414459B CN114414459B CN202210000317.XA CN202210000317A CN114414459B CN 114414459 B CN114414459 B CN 114414459B CN 202210000317 A CN202210000317 A CN 202210000317A CN 114414459 B CN114414459 B CN 114414459B
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- 238000002360 preparation method Methods 0.000 title claims abstract description 32
- 238000000684 flow cytometry Methods 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 15
- 230000007246 mechanism Effects 0.000 claims abstract description 8
- 210000004027 cell Anatomy 0.000 claims description 27
- 238000007789 sealing Methods 0.000 claims description 16
- 238000003825 pressing Methods 0.000 claims description 14
- 238000010438 heat treatment Methods 0.000 claims description 13
- 210000003855 cell nucleus Anatomy 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 7
- 239000000284 extract Substances 0.000 claims description 5
- 230000009471 action Effects 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 3
- 210000004940 nucleus Anatomy 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 230000008859 change Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 210000003463 organelle Anatomy 0.000 description 4
- 238000005299 abrasion Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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Abstract
The invention discloses an intelligent and efficient sample preparation system for flow cytometry and a use method thereof, and belongs to the technical field of flow cytometry. The intelligent sample preparation device for the flow cytometry comprises a barrel, wherein an intelligent temperature control system is arranged in the barrel, an outlet end of the barrel is in threaded connection with a centrifuge tube, a first filter screen plate is arranged on the inner wall of the barrel, a second filter screen plate is also arranged in the barrel, the second filter screen plate is arranged below the first filter screen plate, the second filter screen plate and the first filter screen plate are movably abutted, and an adjusting mechanism is arranged on the barrel; the invention has simple operation, the second filter screen plate is rotated by the adjusting mechanism, further the second filter screen plate is staggered with the filter screen holes of the first filter screen plate, the size of the filter screen holes is changed, the adjustment can be carried out according to the use condition, different cells can be filtered without changing different cylinders, and the preparation efficiency of staff is further improved.
Description
Technical Field
The invention relates to the technical field of flow cytometry, in particular to an intelligent and efficient sample preparation system for flow cytometry and a use method thereof.
Background
The flow cytometry is a detection means for quantitatively analyzing and sorting single cells or other biological particles at the function level, has the advantages of high speed, high precision, good accuracy and the like, becomes the most advanced cell quantitative analysis technology in the current generation, can rapidly determine the biological properties of single cells or organelles, can separate and collect specific cells or organelles from a population, can rapidly analyze tens of thousands of cells, and can simultaneously measure a plurality of parameters from one cell.
Flow cytometry is an important analytical instrument that is widely used in medical, biological, and agricultural fields, and when separating cells, nuclei, or other organelles from multicellular organisms for flow cytometry analysis, it is often necessary to filter a sample to remove large-particle impurities, and the solution containing the desired cells or organelles obtained by filtration generally needs to be collected by centrifugation.
When the conventional cell filter for flow cytometer sample preparation is used, the filtrate is required to be filtered into a centrifuge tube, and when the cell or the cell nucleus is filtered, the required filter screens are different in size and shape, so that different filter screens are required to be replaced frequently, and when the filter screens are replaced, the whole filter cartridge is required to be replaced, so that inconvenience is caused, and the preparation efficiency of staff is affected.
Disclosure of Invention
The invention aims to solve the problems in the prior art, and provides an intelligent and efficient sample preparation system for flow cytometry and a use method thereof.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a intelligent preparation facilities of sample for flow cytometry, the intelligent temperature control system is provided with in the barrel, the exit end threaded connection of barrel has the centrifuging tube, the barrel inner wall is provided with first filter screen plate, still be provided with the second filter screen plate in the barrel, the below of first filter screen plate is arranged in to the second filter screen plate, second filter screen plate and first filter screen plate activity offset, be provided with adjustment mechanism on the barrel, adjustment mechanism is including setting firmly auxiliary tube, the rotatory rotating assembly of drive auxiliary tube around barrel axle center and the lifting unit of drive auxiliary tube along axial displacement on the second filter screen plate, the outer wall of auxiliary tube offsets with the inner wall activity of centrifuging tube.
Preferably, the rotating assembly comprises a gear ring fixedly arranged on the auxiliary pipe, a gear groove used for the movement of the gear ring is formed in the cylinder, a movable gear is connected to the outer wall of the gear ring in a meshed mode, a rotating rod is connected to the outer wall of the movable gear and arranged on the cylinder through a connecting block, a worm wheel is connected to one end, far away from the movable gear, of the rotating rod, the rotating assembly further comprises a supporting block fixedly arranged on the outer wall of the cylinder, a rotating rod is connected to the supporting block in a rotating mode through a bearing, and a worm meshed with the worm wheel is connected to the outer wall of the rotating rod.
Preferably, the lifting assembly comprises a first piston which is slidably connected in the rotary rod, the outer wall of the first piston is connected with a push rod, the push rod is slidably connected in the rotary rod, one end, far away from the first piston, of the push rod penetrates through the rotary rod and is connected with a push plate, the outer wall of the push rod is sleeved with a first elastic element, and two ends of the first elastic element are respectively connected with the rotary rod and the push plate.
Preferably, the lifting assembly further comprises a fixed ring fixedly arranged on the inner wall of the cylinder body, the outer wall of the fixed ring is connected with a sealing shell, a first air duct is communicated between the sealing shell and the rotating rod, the inner wall of the sealing shell is connected with a second elastic element, one end, far away from the bottom wall of the sealing shell, of the second elastic element is connected with a second piston, the outer wall of the second piston is connected with a push rod, one end, far away from the second piston, of the push rod penetrates the sealing shell and extends outwards, and the outer wall of the auxiliary pipe is fixedly connected with a connecting plate which is movably propped against the push rod.
Preferably, the barrel inner wall has seted up the spout, spout inner wall is connected with third elastic element, the one end that the spout inner wall was kept away from to third elastic element is connected with the slider, the slider links to each other with first filter screen plate is fixed, the supporting shoe is connected with the baffle through the round pin axle rotation.
Preferably, the first filter screen plate comprises a first filter screen frame and a first screen body arranged on the inner wall of the first filter screen frame, the second filter screen plate comprises a second filter screen frame and a second screen body arranged on the inner wall of the second filter screen frame, the first screen body and the second screen body are movably abutted against each other, the bottom wall of the first filter screen frame is provided with a groove, and the top of the second filter screen frame is connected with a bump matched with the groove.
Preferably, a first cavity is arranged in the push plate, a fourth elastic element is connected to the inner wall of the first cavity, a third piston is connected to one end, far away from the inner wall of the first cavity, of the fourth elastic element, a connecting rod is connected to the outer wall of the third piston, and a pressing plate is connected to one end, far away from the third piston, of the connecting rod.
Preferably, a second cavity is formed in the supporting block, a second air duct is connected between the second cavity and the first cavity, a fifth elastic element is connected to the inner wall of the second cavity, one end, far away from the inner wall of the second cavity, of the fifth elastic element is connected with a fourth piston, a rack plate is connected to the outer wall of the fourth piston, the rack plate is slidably connected in the supporting block, and a half gear meshed with the rack plate is connected to the outer wall of the baffle.
Preferably, the intelligent temperature control system comprises a control module, a refrigerating sheet, a heating sheet and a temperature sensor, wherein the temperature sensor is used for collecting temperature signals inside the cylinder, the refrigerating sheet and the heating sheet are respectively used for cooling and heating the inside of the cylinder, the control module is used for collecting and processing the signals collected by the temperature sensor, and the refrigerating sheet, the heating sheet and the temperature sensor are electrically connected with the control module.
The invention also discloses a use method of the sample intelligent preparation device for flow cytometry, which comprises the following steps:
s1: when the device is used, the cylinder is fixed on the centrifugal frame, the centrifugal tube is rotated, so that the centrifugal tube is screwed at the bottom of the cylinder, the top of the centrifugal tube is arranged between the cylinder and the auxiliary tube, the top of the cylinder is conical, the material is convenient to pour, the first filter screen plate and the second filter screen plate are propped against each other, the meshes of the first filter screen plate and the second filter screen plate are consistent and coincide, and liquid is poured from the top of the cylinder during filtration and then filtered by the filter screen plate, and enters the inside of the centrifugal tube;
s2: when the small-size cells or cell nuclei need to be filtered, a worker holds the push plate, applies force to the pressure plate on the push plate, and the third piston in the first cavity extrudes the internal air, so that the fourth piston in the second cavity pushes the rack plate to be meshed with the half gear of the baffle plate, the baffle plate does not resist the push plate any more, and the push plate can be pulled in a direction far away from the rotating rod at the moment;
s3: when the push plate moves, the push rod is driven to move, the first elastic element which is originally compressed starts to recover and stretch, so that the push rod drives the first piston to move in the rotary rod, the first piston extracts air in the sealed shell through the first air duct, the inside of the sealed shell becomes negative pressure, the second piston moves downwards, the stretched second elastic element recovers and is compressed, at the moment, the ejector rod does not act on the connecting plate of the auxiliary pipe any more, and the auxiliary pipe drives the second filter screen plate to move downwards and not to prop against the first filter screen plate any more;
s4: the push plate can be rotated at the moment, the push plate drives the rotary rod to rotate in the supporting block through the push rod, and then the worm on the outer side of the rotary rod is meshed with the worm wheel of the rotary rod, so that the rotary rod drives the movable gear to be meshed with the gear ring on the outer side of the auxiliary pipe, and at the moment, the second filter screen plate rotates relative to the first filter screen plate, so that the originally overlapped meshes are staggered;
s5: after the second filter screen plate is adjusted, pushing the push plate to the rotary rod, and changing the first elastic element from a stretching state to a compressed state again, so that the first piston extrudes air into the sealing shell again, changing the second elastic element to a stretching state again, pushing the ejector rod to press the connecting plate upwards by the second piston, and enabling the connecting plate to drive the second filter screen plate to tightly press against the first filter screen plate through the auxiliary pipe, so that the overlapped filter screen holes can filter cells or cell nuclei with smaller sizes;
s6: then the pressing of fingers on the pressing plate is released, and under the action of the elasticity of the fourth elastic element and the fifth elastic element, the rack plate is reversely meshed with the half gear, so that the half gear drives the baffle to rotate and limits and resists the pushing plate again, and the second filter screen plate is tightly abutted with the first filter screen plate.
Compared with the prior art, the invention provides an intelligent and efficient sample preparation system for flow cytometry and a use method thereof, and the intelligent and efficient sample preparation system has the following beneficial effects:
1. this a sample intelligent high-efficient preparation system for flow cytometry and application method thereof makes the second filter screen board rotate through adjustment mechanism, and then makes the second filter screen board crisscross with the filter screen hole of first filter screen board, changes the filter screen hole size, can adjust according to the in service behavior, easy operation, need not change different barrels and can filter different cells, and then improves staff's preparation efficiency.
2. This a sample intelligent high-efficient preparation system for flow cytometry and application method thereof, through the clamp plate effort on to the push pedal, make the inside air of third piston extrusion in the first cavity, and then make the inside fourth piston of second cavity promote rack board and the half gear engagement of baffle, make the baffle no longer keep out the push pedal, can be with the push pedal to the direction pulling of keeping away from the rotary rod this moment, conveniently carry out tensile removal to the push pedal.
3. According to the sample intelligent high-efficiency preparation system for the flow cytometry and the application method thereof, when the push plate moves, the push rod is driven to move, the first elastic element which is originally compressed starts to recover and stretch, the push rod drives the first piston to move in the rotating rod, the first piston extracts air in the sealed shell through the first air duct, the inside of the sealed shell becomes negative pressure, the second piston moves downwards, the stretched second elastic element recovers and is compressed, at the moment, the ejector rod does not act on the connecting plate of the auxiliary pipe any more, the auxiliary pipe drives the second filter screen plate to move downwards and not to abut against the first filter screen plate any more, and when the second filter screen plate is prevented from rotating, the second filter screen plate is tightly abutted against the first filter screen plate due to the fact that abrasion between the first filter screen plate and the second filter screen plate is serious, and the service life of the filter screen plate is reduced.
4. This a sample intelligent high-efficient preparation system for flow cytometry and application method thereof, through rotating the push pedal, make the push pedal pass through the push rod and drive the rotary rod and rotate in the supporting shoe, and then make the worm in the rotary rod outside and the worm wheel intermeshing of rotary rod to make the rotary rod drive the gear ring meshing in the outside of movable gear and auxiliary pipe, the second filter screen board rotates for first filter screen board this moment, makes the mesh that coincides originally begin to be crisscross, the mesh diminishes, and then filters cell or cell nucleus of less size.
5. According to the sample intelligent high-efficiency preparation system for the flow cytometry and the application method thereof, during the process that the baffle is propped against the push plate, the push plate is pushed to the rotary rod, so that the first piston extrudes air to the sealing shell through the first air guide pipe, the second piston drives the ejector rod to ascend again, the second elastic element stretches secondarily, the third elastic element is compressed, the first filter screen plate and the second filter screen plate which are propped against each other synchronously shake up and down, the filtering effect of the filter screen holes is improved, the accumulation blocking of trapped cells and impurities is avoided, and the circulation state of the filter screen holes is ensured.
Drawings
FIG. 1 is a schematic diagram of a first embodiment of the present invention;
FIG. 2 is an enlarged schematic view of part A of FIG. 1 in accordance with the present invention;
FIG. 3 is a second schematic diagram of the structure of the present invention;
FIG. 4 is an enlarged schematic view of part B of FIG. 3 in accordance with the present invention;
FIG. 5 is a schematic cross-sectional view of the present invention;
FIG. 6 is a schematic cross-sectional view of a barrel according to the present invention;
FIG. 7 is a schematic view of the auxiliary tube according to the present invention;
FIG. 8 is a schematic diagram of the structure of a first screen plate and a second screen plate of the present invention;
FIG. 9 is a schematic cross-sectional view of a rotary rod according to the present invention;
FIG. 10 is a schematic cross-sectional view of a seal housing of the present invention;
FIG. 11 is a schematic cross-sectional view of a push plate according to the present invention;
FIG. 12 is a schematic cross-sectional view of a support base according to the present invention;
FIG. 13 is a functional block diagram of an intelligent temperature control system of the present invention.
In the figure: 1. a cylinder; 2. centrifuging tube; 3. a first screen plate; 301. a first filter screen frame; 3011. a groove; 302. a first net body; 4. a second screen plate; 401. a second filter screen frame; 4011. a bump; 402. a second net body; 5. an auxiliary tube; 501. a gear ring; 502. a connecting plate; 6. a gear groove; 7. a rotating lever; 701. a movable gear; 702. a worm wheel; 8. a support block; 9. a rotating rod; 901. a first piston; 902. a push rod; 903. a push plate; 904. a first elastic element; 10. a worm; 11. a fixing ring; 12. a seal housing; 121. a second elastic element; 122. a second piston; 123. a push rod; 13. a first air duct; 14. a chute; 141. a third elastic element; 142. a slide block; 15. a baffle; 151. a half gear; 16. a first cavity; 161. a fourth elastic element; 162. a third piston; 163. a connecting rod; 164. a pressing plate; 17. a second cavity; 171. a fifth elastic element; 172. a fourth piston; 173. rack plate; 18. and a second air duct.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments.
In the description of the present invention, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present invention and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
Example 1:
referring to fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, fig. 6, fig. 7 and fig. 8, a sample intelligent preparation device for flow cytometry comprises a barrel 1, an intelligent temperature control system is arranged in the barrel 1, an outlet end of the barrel 1 is in threaded connection with a centrifuge tube 2, a first filter screen plate 3 is arranged on the inner wall of the barrel 1, a second filter screen plate 4 is further arranged in the barrel 1, the second filter screen plate 4 is arranged below the first filter screen plate 3, the second filter screen plate 4 and the first filter screen plate 3 are movably abutted against each other, an adjusting mechanism is arranged on the barrel 1 and comprises an auxiliary tube 5 fixedly arranged on the second filter screen plate 4, a rotating assembly for driving the auxiliary tube 5 to rotate around the axis of the barrel 1 and a lifting assembly for driving the auxiliary tube 5 to axially move, and the outer wall of the auxiliary tube 5 is movably abutted against the inner wall of the centrifuge tube 2.
Specifically, when the device is used, firstly, fix barrel 1 on the centrifuge frame, rotate centrifuging tube 2, make centrifuging tube 2 connect soon in the bottom of barrel 1, and at this moment, barrel 1 is arranged in at the top of centrifuging tube 2 and auxiliary tube 5 between, barrel 1's top sets up to the toper, be convenient for fall material, first filter screen plate 3 offsets with second filter screen plate 4 in the beginning, and first filter screen plate 3 and the mesh of second filter screen plate 4 are unanimous and coincide, liquid pours from barrel 1 top during the filtration, afterwards filter through the filter screen plate, get into centrifuging tube 2's inside, when needs to filter cells or the nucleus of less size, make second filter screen plate 4 rotate through adjustment mechanism, and then make second filter screen plate 4 crisscross with the filter screen hole of first filter screen plate 3, change the filter screen hole size, can adjust according to the in service behavior, can filter different cells to different barrel 1 of not need to change, and then improve staff's preparation efficiency.
Example 2:
referring to fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, fig. 6 and fig. 7, a sample intelligent preparation device for flow cytometry is the same as that of embodiment 1, further, a rotating assembly comprises a gear ring 501 fixedly arranged on an auxiliary tube 5, a gear groove 6 for the movement of the gear ring 501 is formed in a cylinder 1, a movable gear 701 is connected with the outer wall of the gear ring 501 in a meshed manner, a rotating rod 7 is connected with the outer wall of the movable gear 701, the rotating rod 7 is arranged on the cylinder 1 through a connecting block, a worm wheel 702 is connected with one end, far away from the movable gear 701, of the rotating rod 7, the rotating assembly further comprises a supporting block 8 fixedly arranged on the outer wall of the cylinder 1, a rotating rod 9 is rotatably connected in the supporting block 8 through a bearing, and a worm 10 meshed with the worm wheel 702 is connected with the outer wall of the rotating rod 9.
Specifically, through rotating rotary rod 9, make worm 10 and the worm wheel 702 intermeshing of rotary rod 7 in the rotary rod 9 outside to make rotary rod 7 drive the gear ring 501 meshing in the outside of gear 701 and auxiliary pipe 5, second filter screen plate 4 rotates for first filter screen plate 3 this moment, make originally coincident mesh begin to stagger, the mesh of straining after staggering can filter less size cell or nucleus, need not change different barrel 1 and can filter different cells, easy operation is convenient for improve staff's preparation efficiency.
Example 3:
referring to fig. 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10, the sample intelligent preparation apparatus for flow cytometry is the same as that of embodiment 2, further, the lifting assembly comprises a first piston 901 slidably connected in the rotary rod 9, a push rod 902 is connected to an outer wall of the first piston 901, one end of the push rod 902 far away from the first piston 901 in the rotary rod 9 passes through the rotary rod 9 and is connected with a push plate 903, a first elastic element 904 is sleeved on an outer wall of the push rod 902, and two ends of the first elastic element 904 are respectively connected with the rotary rod 9 and the push plate 903.
Further, lifting assembly still includes the solid fixed ring 11 who sets firmly at barrel 1 inner wall, fixed ring 11 outer wall connection has sealed casing 12, the intercommunication has first air duct 13 between sealed casing 12 and the rotary rod 9, sealed casing 12 inner wall connection has second elastic element 121, the one end that sealed casing 12 diapire was kept away from to second elastic element 121 is connected with second piston 122, second piston 122 outer wall connection has ejector pin 123, the one end that second piston 122 was kept away from to ejector pin 123 passes sealed casing 12 and extends to the outside, auxiliary tube 5 outer wall fixedly connected with and ejector pin 123 activity offset connecting plate 502.
Specifically, when the smaller size cells or nuclei need to be filtered, the worker pulls the push plate 903 away from the rotating rod 9, the push plate 903 drives the push rod 902 to move when moving, the first elastic element 904 which is originally compressed starts to recover and stretch, so that the push rod 902 drives the first piston 901 to move in the rotating rod 9, the first piston 901 extracts the air in the sealed shell 12 through the first air duct 13, the inside of the sealed shell 12 becomes negative pressure, the second piston 122 moves downwards, the stretched second elastic element 121 recovers and is compressed, at this moment, the ejector rod 123 no longer acts on the connecting plate 502 of the auxiliary tube 5, the auxiliary tube 5 drives the second filter screen 4 to move downwards and no longer abuts against the first filter screen 3, and when the second filter screen 4 is prevented from rotating, the second filter screen 4 abuts against the first filter screen 3 tightly, so that the abrasion between the first filter screen 3 and the second filter screen 4 is serious, and the service life of the second filter screen 4 is reduced.
Example 4:
referring to fig. 6, 7, 8, 9, 10, 11 and 12, the sample intelligentized preparation apparatus for flow cytometry is the same as that of embodiment 3, further, a chute 14 is formed on the inner wall of the barrel 1, a third elastic element 141 is connected to the inner wall of the chute 14, one end of the third elastic element 141 far away from the inner wall of the chute 14 is connected with a sliding block 142, the sliding block 142 is fixedly connected with the first screen 3, and the supporting block 8 is rotatably connected with a baffle 15 through a pin shaft.
Further, the first filter screen plate 3 comprises a first filter screen frame 301 and a first screen body 302 arranged on the inner wall of the first filter screen frame 301, the second filter screen plate 4 comprises a second filter screen frame 401 and a second screen body 402 arranged on the inner wall of the second filter screen frame 401, the first screen body 302 is movably abutted against the second screen body 402, a groove 3011 is formed in the bottom wall of the first filter screen frame 301, and a projection 4011 matched with the groove 3011 is connected to the top of the second filter screen frame 401.
Further, a first cavity 16 is disposed in the push plate 903, a fourth elastic element 161 is connected to an inner wall of the first cavity 16, a third piston 162 is connected to an end of the fourth elastic element 161 away from the inner wall of the first cavity 16, a connecting rod 163 is connected to an outer wall of the third piston 162, and a pressing plate 164 is connected to an end of the connecting rod 163 away from the third piston 162.
Further, a second cavity 17 is arranged in the supporting block 8, a second air duct 18 is connected between the second cavity 17 and the first cavity 16, a fifth elastic element 171 is connected to the inner wall of the second cavity 17, one end, far away from the inner wall of the second cavity 17, of the fifth elastic element 171 is connected with a fourth piston 172, the outer wall of the fourth piston 172 is connected with a rack plate 173, the rack plate 173 is slidably connected in the supporting block 8, and the outer wall of the baffle 15 is connected with a half gear 151 meshed with the rack plate 173.
Specifically, when the small-size cells or nuclei need to be filtered, the staff holds the push plate 903, and applies force to the pressing plate 164 on the push plate 903, the third piston 162 in the first cavity 16 extrudes the internal air, and then the fourth piston 172 in the second cavity 17 pushes the rack plate 173 to be meshed with the half gear 151 of the baffle 15, so that the baffle 15 does not resist the push plate 903, at this time, the push plate 903 can be pulled in a direction away from the rotating rod 9, the lifting assembly is adjusted, after the second filter screen plate 4 is adjusted, the pressing of the pressing plate 164 by the fingers is released, under the action of the elastic force of the fourth elastic element 161 and the fifth elastic element 171, the rack plate 173 is reversely meshed with the half gear 151, the half gear 151 drives the baffle 15 to rotate and limits and resists the push plate again, the second filter screen plate 4 is tightly abutted against the first filter screen plate 3, the filter screen hole structure of the two layers 903 after the adjustment is ensured to be stable, and on the basis, the first piston 903 pushes the push plate 903 to the rotating rod 9, the air is pushed by the first piston 903 to pass through the first air guide tube 13 to the second filter screen plate 12, the second filter screen plate 122 is driven by the second elastic element 122 to resist the second filter screen plate 122, the net hole is prevented from being compressed, the net hole is prevented from being accumulated by the second filter screen plate 122, the net hole is prevented from being compressed, the net hole is synchronously pressed by the second filter screen plate is compressed, and the filter screen plate is prevented from being stacked, the net hole filter screen plate is pressed, and the filter screen plate is pressed.
Example 5:
referring to fig. 4 and 13, the sample intelligent preparation device for flow cytometry is the same as that of embodiment 2, and further, the intelligent temperature control system comprises a control module, a refrigerating plate, a heating plate and a temperature sensor, wherein the temperature sensor is used for collecting temperature signals inside the cylinder 1, the refrigerating plate and the heating plate are respectively used for cooling and heating the inside of the cylinder 1, the control module is used for collecting and processing the signals collected by the temperature sensor, and the refrigerating plate, the heating plate and the temperature sensor are electrically connected with the control module.
Specifically, gather the inside temperature signal of barrel 1 through temperature sensor, control module analyzes and handles the signal of gathering, when the temperature is lower, control heating plate work, when the temperature is higher, control refrigeration piece work, and then guarantee that the cell is in suitable temperature all the time, avoid fluctuation and the unstability of cell function detection data that the uncontrollable temperature caused.
The invention also discloses a use method of the sample intelligent preparation device for flow cytometry, which comprises the following steps:
s1: when the device is used, firstly, the cylinder 1 is fixed on a centrifugal frame, the centrifugal tube 2 is rotated, so that the centrifugal tube 2 is screwed at the bottom of the cylinder 1, at the moment, the top of the centrifugal tube 2 is arranged between the cylinder 1 and the auxiliary tube 5, the top of the cylinder 1 is conical, the material is convenient to pour, at the moment, the first filter screen plate 3 is propped against the second filter screen plate 4, the meshes of the first filter screen plate 3 and the second filter screen plate 4 are consistent and coincide, and liquid is poured from the top of the cylinder 1 during filtration, and then is filtered by the filter screen plates and enters the inside of the centrifugal tube 2;
s2: when the smaller size cells or nuclei need to be filtered, a worker holds the push plate 903 and applies force to the pressure plate 164 on the push plate 903, the third piston 162 in the first cavity 16 extrudes the internal air, so that the fourth piston 172 in the second cavity 17 pushes the rack plate 173 to engage with the half gear 151 of the baffle 15, the baffle 15 does not resist the push plate 903 any more, and the push plate 903 can be pulled in a direction away from the rotating rod 9;
s3: when the push plate 903 moves, the push rod 902 is driven to move, the first elastic element 904 which is originally compressed starts to recover and stretch, so that the push rod 902 drives the first piston 901 to move in the rotary rod 9, the first piston 901 extracts air in the sealed shell 12 through the first air duct 13, the inside of the sealed shell 12 becomes negative pressure, the second piston 122 moves downwards, the stretched second elastic element 121 recovers and is compressed, at the moment, the ejector rod 123 does not act on the connecting plate 502 of the auxiliary pipe 5 any more, and the auxiliary pipe 5 drives the second filter screen plate 4 to move downwards and not to be propped against the first filter screen plate 3 any more;
s4: at this time, the push plate 903 can be rotated, so that the push plate 903 drives the rotary rod 9 to rotate in the supporting block 8 through the push rod 902, and further the worm 10 at the outer side of the rotary rod 9 is meshed with the worm wheel 702 of the rotary rod 7, so that the rotary rod 7 drives the movable gear 701 to be meshed with the gear ring 501 at the outer side of the auxiliary pipe 5, and at this time, the second screen plate 4 rotates relative to the first screen plate 3, so that the originally overlapped meshes are staggered;
s5: after the second filter screen plate 4 is adjusted, the push plate 903 is pushed to the rotary rod 9, the first elastic element 904 is changed from a stretching state to a compressed state again, so that the first piston 901 extrudes air into the sealing shell 12 again, the second elastic element 121 is changed to a stretching state again, the second piston 122 pushes the ejector rod 123 to press the connecting plate 502 upwards, the connecting plate 502 drives the second filter screen plate 4 to tightly abut against the first filter screen plate 3 through the auxiliary pipe 5, and the overlapped filter screen holes can filter cells or cell nuclei with smaller sizes;
s6: then the pressing of the pressing plate 164 by the fingers is released, and under the action of the elastic force of the fourth elastic element 161 and the fifth elastic element 171, the rack plate 173 is reversely meshed with the half gear 151, so that the half gear 151 drives the baffle 15 to rotate and limits and resists the pushing plate 903 again, and the second filter screen plate 4 is tightly abutted against the first filter screen plate 3.
According to the invention, the filter screen plate can be adjusted and dithered by the device only by driving the push plate 903, the operation is simple, the device can be adjusted according to the use condition, different cells can be filtered without changing different cylinders, and the preparation efficiency and the filtering effect of staff are improved.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (3)
1. The intelligent sample preparation device for the flow cytometry comprises a barrel (1), wherein an intelligent temperature control system is arranged in the barrel (1), the intelligent sample preparation device is characterized in that an outlet end of the barrel (1) is connected with a centrifuge tube (2) in a threaded mode, a first filter screen plate (3) is arranged on the inner wall of the barrel (1), a second filter screen plate (4) is further arranged in the barrel (1), the second filter screen plate (4) is arranged below the first filter screen plate (3), the second filter screen plate (4) and the first filter screen plate (3) are movably abutted against each other, an adjusting mechanism is arranged on the barrel (1), and comprises an auxiliary tube (5) fixedly arranged on the second filter screen plate (4), a rotating assembly for driving the auxiliary tube (5) to rotate around the axis of the barrel (1) and a lifting assembly for driving the auxiliary tube (5) to move along the axial direction, and the outer wall of the auxiliary tube (5) is movably abutted against the inner wall of the centrifuge tube (2);
the rotary assembly comprises a gear ring (501) fixedly arranged on an auxiliary pipe (5), a gear groove (6) used for enabling the gear ring (501) to move is formed in the cylinder (1), a movable gear (701) is connected to the outer wall of the gear ring (501) in a meshed mode, a rotary rod (7) is connected to the outer wall of the movable gear (701), the rotary rod (7) is arranged on the cylinder (1) through a connecting block, one end, far away from the movable gear (701), of the rotary rod (7) is connected with a worm wheel (702), the rotary assembly further comprises a supporting block (8) fixedly arranged on the outer wall of the cylinder (1), a rotary rod (9) is connected in the supporting block (8) in a rotary mode through a bearing, and a worm (10) meshed with the worm wheel (702) is connected to the outer wall of the rotary rod (9).
The lifting assembly comprises a first piston (901) which is slidably connected in a rotary rod (9), the outer wall of the first piston (901) is connected with a push rod (902), the push rod (902) is slidably connected in the rotary rod (9), one end, far away from the first piston (901), of the push rod (902) penetrates through the rotary rod (9) and is connected with a push plate (903), a first elastic element (904) is sleeved on the outer wall of the push rod (902), and two ends of the first elastic element (904) are respectively connected with the rotary rod (9) and the push plate (903);
the lifting assembly further comprises a fixed ring (11) fixedly arranged on the inner wall of the cylinder body (1), the outer wall of the fixed ring (11) is connected with a sealing shell (12), a first air guide pipe (13) is communicated between the sealing shell (12) and the rotating rod (9), the inner wall of the sealing shell (12) is connected with a second elastic element (121), one end, far away from the bottom wall of the sealing shell (12), of the second elastic element (121) is connected with a second piston (122), the outer wall of the second piston (122) is connected with a push rod (123), one end, far away from the second piston (122), of the push rod (123) penetrates through the sealing shell (12) and extends outwards, and the outer wall of the auxiliary pipe (5) is fixedly connected with a connecting plate (502) which is movably propped against the push rod (123);
a sliding groove (14) is formed in the inner wall of the cylinder body (1), a third elastic element (141) is connected to the inner wall of the sliding groove (14), a sliding block (142) is connected to one end, far away from the inner wall of the sliding groove (14), of the third elastic element (141), the sliding block (142) is fixedly connected with the first screen plate (3), and a baffle (15) is connected to the supporting block (8) through a pin shaft in a rotating mode;
the first filter screen plate (3) comprises a first filter screen frame (301) and a first screen body (302) arranged on the inner wall of the first filter screen frame (301), the second filter screen plate (4) comprises a second filter screen frame (401) and a second screen body (402) arranged on the inner wall of the second filter screen frame (401), the first screen body (302) is movably abutted against the second screen body (402), a groove (3011) is formed in the bottom wall of the first filter screen frame (301), and a lug (4011) matched with the groove (3011) is connected to the top of the second filter screen frame (401);
a first cavity (16) is arranged in the push plate (903), a fourth elastic element (161) is connected to the inner wall of the first cavity (16), a third piston (162) is connected to one end, far away from the inner wall of the first cavity (16), of the fourth elastic element (161), a connecting rod (163) is connected to the outer wall of the third piston (162), and a pressing plate (164) is connected to one end, far away from the third piston (162), of the connecting rod (163);
the novel air guide device is characterized in that a second cavity (17) is formed in the supporting block (8), a second air guide pipe (18) is connected between the second cavity (17) and the first cavity (16), a fifth elastic element (171) is connected to the inner wall of the second cavity (17), a fourth piston (172) is connected to one end, far away from the inner wall of the second cavity (17), of the fifth elastic element (171), a rack plate (173) is connected to the outer wall of the fourth piston (172), the rack plate (173) is slidably connected in the supporting block (8), and a half gear (151) meshed with the rack plate (173) is connected to the outer wall of the baffle (15).
2. The intelligent sample preparation device for flow cytometry according to claim 1, wherein the intelligent temperature control system comprises a control module, a refrigerating plate, a heating plate and a temperature sensor, the temperature sensor is used for collecting temperature signals inside the cylinder (1), the refrigerating plate and the heating plate are respectively used for cooling and heating the inside of the cylinder (1), the control module is used for collecting and processing the signals collected by the temperature sensor, and the refrigerating plate, the heating plate and the temperature sensor are electrically connected with the control module.
3. A method of using the sample intelligent preparation device for flow cytometry of claim 2, comprising the steps of:
s1: when the device is used, firstly, the cylinder body (1) is fixed on the centrifugal frame, the centrifugal tube (2) is rotated, the centrifugal tube (2) is screwed at the bottom of the cylinder body (1), at the moment, the top of the centrifugal tube (2) is arranged between the cylinder body (1) and the auxiliary tube (5), the top of the cylinder body (1) is arranged into a cone shape, the material is convenient to pour, at the moment, the first filter screen plate (3) is propped against the second filter screen plate (4), the meshes of the first filter screen plate (3) and the second filter screen plate (4) are consistent and coincide, and liquid is poured from the top of the cylinder body (1) during filtration, and then is filtered by the filter screen plate and enters the inside of the centrifugal tube (2);
s2: when the small-size cells or cell nuclei need to be filtered, a worker holds the push plate (903) and applies force to the pressing plate (164) on the push plate (903), the third piston (162) in the first cavity (16) extrudes the internal air, so that the fourth piston (172) in the second cavity (17) pushes the rack plate (173) to be meshed with the half gear (151) of the baffle plate (15), the baffle plate (15) does not resist the push plate (903), and the push plate (903) can be pulled in a direction far away from the rotating rod (9);
s3: when the push plate (903) moves, the push rod (902) is driven to move, the first elastic element (904) which is originally compressed starts to recover and stretch, so that the push rod (902) drives the first piston (901) to move in the rotary rod (9), the first piston (901) extracts air in the sealing shell (12) through the first air duct (13), the inside of the sealing shell (12) becomes negative pressure, the second piston (122) moves downwards, the stretched second elastic element (121) recovers and is compressed, at the moment, the ejector rod (123) does not apply force to the connecting plate (502) of the auxiliary pipe (5), and the auxiliary pipe (5) drives the second filter screen plate (4) to move downwards and not abut against the first filter screen plate (3);
s4: the push plate (903) can be rotated at the moment, the push plate (903) drives the rotary rod (9) to rotate in the supporting block (8) through the push rod (902), and then the worm (10) at the outer side of the rotary rod (9) is meshed with the worm wheel (702) of the rotary rod (7), so that the rotary rod (7) drives the driving gear (701) to be meshed with the gear ring (501) at the outer side of the auxiliary pipe (5), and at the moment, the second screen plate (4) rotates relative to the first screen plate (3), so that the originally overlapped meshes are staggered;
s5: after the second filter screen plate (4) is adjusted, pushing the push plate (903) to the rotary rod (9), changing the first elastic element (904) from a stretching state to a compressed state again, enabling the first piston (901) to squeeze air into the sealing shell (12) again, changing the second elastic element (121) to a stretching state again, pushing the ejector rod (123) to press the connecting plate (502) upwards by the second piston (122), enabling the connecting plate (502) to drive the second filter screen plate (4) to tightly abut against the first filter screen plate (3) through the auxiliary pipe (5), and enabling the overlapped filter screen holes to filter cells or cell nuclei with smaller sizes;
s6: then the pressing of fingers on the pressing plate (164) is released, and under the elastic action of the fourth elastic element (161) and the fifth elastic element (171), the rack plate (173) is reversely meshed with the half gear (151), so that the half gear (151) drives the baffle plate (15) to rotate and limits and withstands the pushing plate (903) again, and the second filter screen plate (4) is tightly abutted against the first filter screen plate (3).
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| CN207488048U (en) * | 2017-12-04 | 2018-06-12 | 浙江大学 | Flow cytometer sample preparation cell filter |
| CN207918840U (en) * | 2018-01-29 | 2018-09-28 | 天津博奥科生物科技有限公司 | Cellular filter |
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| CN211227132U (en) * | 2019-11-06 | 2020-08-11 | 肖兴安 | Autologous blood living cell filtering device |
| CN215050196U (en) * | 2021-04-06 | 2021-12-07 | 天津金域医学检验实验室有限公司 | Cell filter for sample preparation of flow cytometer |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN207488048U (en) * | 2017-12-04 | 2018-06-12 | 浙江大学 | Flow cytometer sample preparation cell filter |
| CN207918840U (en) * | 2018-01-29 | 2018-09-28 | 天津博奥科生物科技有限公司 | Cellular filter |
| CN208717324U (en) * | 2018-06-06 | 2019-04-09 | 上海钰森生物技术有限公司 | A kind of multi-layer cellular strainer |
| CN211227132U (en) * | 2019-11-06 | 2020-08-11 | 肖兴安 | Autologous blood living cell filtering device |
| CN215050196U (en) * | 2021-04-06 | 2021-12-07 | 天津金域医学检验实验室有限公司 | Cell filter for sample preparation of flow cytometer |
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