CN215050196U - Cell filter for sample preparation of flow cytometer - Google Patents

Cell filter for sample preparation of flow cytometer Download PDF

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Publication number
CN215050196U
CN215050196U CN202120692607.6U CN202120692607U CN215050196U CN 215050196 U CN215050196 U CN 215050196U CN 202120692607 U CN202120692607 U CN 202120692607U CN 215050196 U CN215050196 U CN 215050196U
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China
Prior art keywords
barrel
plate
mounting
flow cytometer
sample preparation
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CN202120692607.6U
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Chinese (zh)
Inventor
楚玉兰
周剑峰
贾晓冬
李行
陈宝荣
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Tianjin Jinyu Medical Laboratory Co ltd
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Tianjin Jinyu Medical Laboratory Co ltd
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Abstract

The utility model relates to a cell filter technical field specifically is a cell filter for flow cytometer sample preparation, the novel nipple rectifier comprises a cylindrical shel, the mounting groove has been seted up to the inside of barrel, the inside fixedly connected with connecting axle of mounting groove, the surface swing joint of connecting axle has the rotor plate, the bottom fixedly connected with connecting plate of barrel, the inside swing joint of connecting plate has the mounting panel, the bottom fixedly connected with centrifuging tube of mounting panel. The utility model has the advantages that: through setting up the barrel, when using the barrel, because mounting groove and rotor plate pass through connecting axle swing joint, rotate the inside rotor plate of mounting groove, make the rotor plate be located the inside two corresponding mounting grooves of barrel, rotor plate and the equal level of filter screen are located the inside of barrel this moment, are convenient for filter, and the filter screen interval of the inside filter screen of barrel is all inequality, can adjust according to the in service behavior, need not change different barrels, the operation of being convenient for.

Description

Cell filter for sample preparation of flow cytometer
Technical Field
The utility model relates to a cell filter technical field, especially a cell filter is used in preparation of flow cytometer sample.
Background
Flow cytometry is an important analytical instrument and widely applied in the fields of medicine, biology, agriculture and the like, when a flow cytometer is used for analyzing cells, cell nucleuses or other organelles separated from a multicellular organism, a sample is often required to be filtered so as to remove large-particle impurities, and a solution containing target cells or organelles obtained by filtering is generally required to be centrifuged to collect the required cells or organelles.
The disadvantages of the current cell filters for flow cytometer sample preparation are:
1. when the cell filter for sample preparation of the existing flow cytometer is used, filtrate needs to be filtered into a centrifuge tube, and when the cell filter is used for filtering, different filter screens need to be frequently replaced due to different sizes and shapes of cells or cell nuclei, and the whole filter cartridge needs to be replaced when the filter screens are replaced, so that inconvenience is caused;
2. present cell filter for flow cytometer sample preparation passes through the inside of filter screen entering centrifuging tube with the inside solution of barrel when using, and the barrel is located the top of centrifuging tube, all is provided with a barrel on every centrifuging tube, is not convenient for take out the liquid that filters.
SUMMERY OF THE UTILITY MODEL
The utility model aims to overcome prior art's shortcoming, provide a cell filter for flow cytometer sample preparation.
The purpose of the utility model is realized through the following technical scheme: a cell filter for sample preparation of a flow cytometer comprises a cylinder body, wherein an installation groove is formed in the cylinder body, a connecting shaft is fixedly connected inside the installation groove, and the outer surface of the connecting shaft is movably connected with a rotating plate;
the bottom fixedly connected with connecting plate of barrel, the inside swing joint of connecting plate has the mounting panel, the bottom fixedly connected with centrifuging tube of mounting panel.
Optionally, the inside set up threaded hole of connecting plate, the surface of mounting panel is provided with the external screw thread with screw hole looks adaptation, the height of connecting plate and mounting panel equals, mounting panel swing joint is in the bottom of barrel, when fixing centrifuging tube and barrel, removes the bottom of centrifuging tube to barrel, rotates the centrifuging tube, makes the mounting panel be located the inside of connecting plate, fixes the centrifuging tube in the bottom of barrel this moment, makes a barrel be fit for different centrifuging tubes, practices thrift the cost.
Optionally, a square through hole is formed in the barrel, four mounting grooves are formed in the barrel and are respectively located on four faces of the square through hole, and the barrel is cylindrical in outer portion and convenient to take and store.
Optionally, the width of rotor plate is less than the degree of depth of mounting groove, the length of rotor plate is greater than the distance between the inside two relative mounting grooves of barrel, fixes the rotor plate in the inside of mounting groove after, removes any rotor plate among them, can make the rotor plate be located between two corresponding two mounting grooves, is convenient for fix the rotor plate in the inside of barrel, makes rotor plate and filter screen be the level in the inside of barrel and places, is convenient for filter.
Optionally, the mounting hole has been seted up to the inside of rotor plate, the inside fixedly connected with filter screen of mounting hole, the diameter of filter screen is less than the diameter of centrifuging tube, when using the filter screen to filter, because the centrifuging tube is located the bottom of filter screen for solution can directly get into the inside of centrifuging tube, avoids the inner wall contact of solution and barrel, reduces the clearance number of times, excellent in use effect.
Optionally, the inside swing joint of mounting hole has a feeding section of thick bamboo, the top fixedly connected with limiting plate of a feeding section of thick bamboo, a feeding section of thick bamboo swing joint is in the inside of barrel, fixes a feeding section of thick bamboo in the inside of barrel, lies in the top of barrel through the limiting plate, carries on spacingly to a feeding section of thick bamboo, is convenient for carry solution to the inside of barrel.
Optionally, the top fixedly connected with locating plate of barrel, limiting plate swing joint is in the inside of locating plate, limiting plate swing joint is in the top of barrel, fixes a position the limiting plate, when making a feeding section of thick bamboo remove to the inside of barrel, directly is located the top of filter screen, is convenient for install.
Optionally, the top fixedly connected with feed inlet of limiting plate, the feed inlet is the toper setting, is convenient for pay-off.
Optionally, the rotor plate is provided with four, four the inside of rotor plate all is provided with the filter screen, four the filter screen interval of filter screen is all inequality, because cell or cell nucleus size and shape differ, can select suitable filter screen according to the condition of filtering liquid, need not change different barrels, saves operating procedure, improves work efficiency.
Optionally, the inside of the mounting groove and one side of the rotating plate close to the mounting groove are both provided with a magnetic block, wherein the magnetic block on the rotating plate inside each mounting groove is different from the magnetic block on the rotating plate inside the mounting groove, so that the rotating plate is conveniently fixed inside the mounting groove.
The utility model has the advantages of it is following:
1. this cell filter is used in preparation of flow cytometer sample, through setting up the barrel, the square through hole has been seted up to barrel inside, four mounting grooves are located four faces of square through hole respectively, make all be provided with a rotor plate on four inner walls of barrel, and the filter screen is located the inside of rotor plate, when using the barrel, because mounting groove and rotor plate pass through connecting axle swing joint, rotate the inside rotor plate of mounting groove, make the rotor plate be located two inside corresponding mounting grooves of barrel, rotor plate and filter screen level are located the inside of barrel this moment, be convenient for filter, and the filter screen interval of the inside filter screen of barrel is all inequality, can adjust according to the in service behavior, need not change different barrels, be convenient for operate.
2. This cell filter is used in preparation of flow cytometer sample, through setting up connecting plate and mounting panel, bottom fixedly connected with connecting plate at the barrel, top fixedly connected with mounting panel at the centrifuging tube, wherein the inside threaded hole that has seted up of connecting plate, the surface of mounting panel is provided with the external screw thread with threaded hole looks adaptation, when using, remove centrifuging tube to the bottom of barrel, rotate the centrifuging tube, make the mounting panel be located the inside of connecting plate, fix the bottom at the barrel with the centrifuging tube this moment, and after filtering the completion, rotate the centrifuging tube, make mounting panel and connecting plate separation, be convenient for installation and dismantlement, be fit for fixing a plurality of different centrifuging tubes, the practicality is good.
Drawings
Fig. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic structural view of the connection plate of the present invention;
FIG. 3 is a schematic structural view of the centrifuge tube of the present invention;
fig. 4 is a schematic structural view of the cylinder of the present invention;
FIG. 5 is a schematic structural view of the feed inlet of the present invention;
fig. 6 is a schematic structural view of the rotating plate of the present invention.
In the figure: 1-barrel body, 2-mounting groove, 3-connecting shaft, 4-rotating plate, 5-connecting plate, 6-mounting plate, 7-centrifuge tube, 8-filter screen, 9-feeding barrel, 10-limiting plate, 11-positioning plate and 12-feeding hole.
Detailed Description
The invention will be further described with reference to the accompanying drawings, but the scope of the invention is not limited to the following description.
As shown in fig. 1-6, a cell filter for sample preparation of a flow cytometer comprises a cylinder 1, wherein an installation groove 2 is formed in the cylinder 1, a connecting shaft 3 is fixedly connected in the installation groove 2, and a rotating plate 4 is movably connected to the outer surface of the connecting shaft 3;
the bottom fixedly connected with connecting plate 5 of barrel 1, the inside swing joint of connecting plate 5 has mounting panel 6, and the bottom fixedly connected with centrifuging tube 7 of mounting panel 6.
As an optional technical solution of the utility model:
threaded hole is seted up to the inside of connecting plate 5, the surface of mounting panel 6 is provided with the external screw thread with threaded hole looks adaptation, connecting plate 5 and mounting panel 6 highly equal, mounting panel 6 swing joint is in the bottom of barrel 1, through rotating mounting panel 6, can fix mounting panel 6 in the inside of connecting plate 5, and then fix centrifuge tube 7 in the bottom of barrel 1, during the dismantlement, only need rotate centrifuge tube 7, can make centrifuge tube 7 and barrel 1 separate, be convenient for installation and dismantlement, easy and simple to handle.
As an optional technical solution of the utility model:
the square through hole is formed in the barrel body 1, the four mounting grooves 2 are arranged, the four mounting grooves 2 are respectively located on four faces of the square through hole, the four rotating plates 4 are conveniently located in the barrel body 1 respectively, and when one rotating plate 4 is used, other three rotating plates cannot cause influence.
As an optional technical solution of the utility model:
the width of rotor plate 4 is less than the degree of depth of mounting groove 2, and the length of rotor plate 4 is greater than the distance between 1 inside two relative mounting grooves 2 of barrel, when rotating 2 inside rotor plates 4 of mounting groove, can make rotor plate 4 be located between two corresponding mounting grooves 2, is convenient for fix rotor plate 4 in the inside of barrel 1, makes rotor plate 4 and filter screen 8 place for the level in the inside of barrel 1, is convenient for filter.
As an optional technical solution of the utility model:
the mounting hole has been seted up to the inside of rotor plate 4, the inside fixedly connected with filter screen 8 of mounting hole, and the diameter of filter screen 8 is less than centrifuge tube 7's diameter, when using filter screen 8 to filter, because centrifuge tube 7 is located filter screen 8's bottom for solution can directly get into centrifuge tube 7's inside, avoids solution and barrel 1's inner wall contact, reduces the clearance number of times, excellent in use effect.
As an optional technical solution of the utility model:
the inside swing joint of mounting hole has a feeding section of thick bamboo 9, and the top fixedly connected with limiting plate 10 of a feeding section of thick bamboo 9, and 9 swing joint of a feeding section of thick bamboo are in the inside of barrel 1, when filtering solution, remove limiting plate 10, make a feeding section of thick bamboo 9 be located the inside of barrel 1, and limiting plate 10 is located the top of barrel 1, fixes a position.
As an optional technical solution of the utility model:
the top fixedly connected with locating plate 11 of barrel 1, limiting plate 10 swing joint is in the inside of locating plate 11, and limiting plate 10 swing joint is in the top of barrel 1, when removing feeding section of thick bamboo 9 to the inside of barrel 1, because the location of locating plate 11 makes limiting plate 10 be located the inside of locating plate 11, makes feeding section of thick bamboo 9 directly be located the top of filter screen 8, the installation of being convenient for.
As an optional technical solution of the utility model:
the top of the limiting plate 10 is fixedly connected with a feeding hole 12, and the feeding hole 12 is arranged in a conical shape, so that the feeding hole 12 is large in upper part and small in lower part, and feeding is facilitated.
As an optional technical solution of the utility model:
the rotating plates 4 are four, the filter screens 8 are arranged in the four rotating plates 4, the filter screen intervals of the four filter screens 8 are different, and therefore the barrel 1 is suitable for filtering various different solutions and is good in practicability.
As an optional technical solution of the utility model:
the inside of mounting groove 2 and the one side that rotor plate 4 is close to mounting groove 2 all are provided with the magnetic path, when using different rotor plates 4, can fix other rotor plates 4 in the inside of mounting groove 2.
The working process of the utility model is as follows: firstly, fixing a barrel body 1 on a centrifugal frame, wherein a square through hole is formed in the barrel body 1, four mounting grooves 2 are respectively positioned on four surfaces of the square through hole, so that a rotating plate 4 is arranged on four inner walls of the barrel body 1, a filter screen 8 is positioned in the rotating plate 4, four filter screens 8 are arranged in the barrel body 1, when the barrel body 1 is used, the rotating plate 4 can rotate as the mounting grooves 2 and the rotating plate 4 are movably connected through a connecting shaft 3, the rotating plate 4 in the mounting groove 2 is rotated, as the length of the rotating plate 4 is greater than the distance between two opposite mounting grooves 2 in the barrel body 1, the rotating plate 4 is positioned on the two corresponding mounting grooves 2 in the barrel body 1, and further the rotating plate 4 is fixed between the two mounting grooves 2, at the moment, the rotating plate 4 and the filter screen 8 are horizontally positioned in the barrel body 1, and are convenient for filtering, the filter screen intervals of the filter screen 8 in the barrel body 1 are different, the filter screen intervals can be adjusted according to the use condition, different barrel bodies do not need to be replaced, the operation is convenient, when the filtration is carried out, the feed inlet 12 is moved, the feed barrel 9 is positioned in the barrel body 1, the limiting plate 10 is positioned at the top of the barrel body 1, the limiting plate 10 is positioned in the positioning plate 11, the feed barrel 9 is positioned at the top of the filter screen 8, the connecting plate 5 is fixedly connected at the bottom of the barrel body 1, the mounting plate 6 is fixedly connected at the top of the centrifugal tube 7, a threaded hole is formed in the connecting plate 5, external threads matched with the threaded hole are arranged on the outer surface of the mounting plate 6, when the centrifugal tube 7 is used, the centrifugal tube 7 is moved to the bottom of the barrel body 1, the mounting plate 6 is positioned at the bottom of the connecting plate 5, the centrifugal tube 7 is rotated to be positioned in the connecting plate 5, and then the centrifugal tube 7 is fixed at the bottom of the barrel body 1, realize the installation to the filter, during the filtration, make solution enter the inside of feed cylinder 9 through feed inlet 12, wherein feed inlet 12 is the toper setting, the convenient pouring, later the material is through the filtration of filter screen 8, enter the inside of centrifuging tube 7, wherein the diameter of filter screen 8 is less than the diameter of centrifuging tube 7, avoid during the filtration, solution and the inner wall contact of barrel 1, can use many times, reduce and clear up this moment, and after filtering, rotate centrifuging tube 7, make mounting panel 6 and connecting plate 5 separate, and then separate barrel 1 and centrifuging tube 7, the convenient installation and dismantlement, be fit for fixing a plurality of different centrifuging tubes 7, the practicality is good, when using different filter screens 8, at first take out feed cylinder 9, and rotate the filter screen 8 that uses before, make filter screen 8 be located the inside of mounting groove 2, wherein the inside of mounting groove 2 and the one side that rotor plate 4 is close to mounting groove 2 all are provided with the magnetic path, be convenient for fix rotor plate 4 in the inside of mounting groove 2, simultaneously, can remove the filter screen 8 of difference to barrel 1 in, continue to filter simple structure, the operation of being convenient for.
In summary, when the cell filter for sample preparation of the flow cytometer is used, by arranging the cylinder 1, a square through hole is formed in the cylinder 1, and the four installation grooves 2 are respectively located on four surfaces of the square through hole, so that the four inner walls of the cylinder 1 are all provided with one rotating plate 4, and the filter screen 8 is located inside the rotating plate 4, when the cylinder 1 is used, because the installation grooves 2 and the rotating plates 4 are movably connected through the connecting shaft 3, the rotating plates 4 in the installation grooves 2 are rotated, so that the rotating plates 4 are located on two corresponding installation grooves 2 in the cylinder 1, and at this time, the rotating plates 4 and the filter screen 8 are horizontally located inside the cylinder 1, so that the filtration is convenient, and the filter screen intervals of the filter screen 8 in the cylinder 1 are different, so that the cell filter screen filter can be adjusted according to use conditions, and different cylinders do not need to be replaced, so that the operation is convenient; through setting up connecting plate 5 and mounting panel 6, bottom fixedly connected with connecting plate 5 at barrel 1, top fixedly connected with mounting panel 6 at centrifuging tube 7, wherein the inside set up threaded hole of connecting plate 5, the surface of mounting panel 6 is provided with the external screw thread with screw hole looks adaptation, when using, remove centrifuging tube 7 to barrel 1's bottom, rotate centrifuging tube 7, make mounting panel 6 be located the inside of connecting plate, fix centrifuging tube 7 in barrel 1's bottom this moment, and after filtering the completion, rotate centrifuging tube 7, make mounting panel 6 and connecting plate 5 separate, be convenient for install and dismantle, be fit for fixing a plurality of different centrifuging tubes 7, the practicality is good.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (10)

1. A cell filter for sample preparation of a flow cytometer, comprising: the drum comprises a drum body (1), wherein an installation groove (2) is formed in the drum body (1), a connecting shaft (3) is fixedly connected in the installation groove (2), and a rotating plate (4) is movably connected to the outer surface of the connecting shaft (3);
the bottom fixedly connected with connecting plate (5) of barrel (1), the inside swing joint of connecting plate (5) has mounting panel (6), the bottom fixedly connected with centrifuging tube (7) of mounting panel (6).
2. A flow cytometer sample preparation cell filter as described in claim 1, wherein: threaded holes are formed in the connecting plate (5), external threads matched with the threaded holes are arranged on the outer surface of the mounting plate (6), the connecting plate (5) and the mounting plate (6) are equal in height, and the mounting plate (6) is movably connected to the bottom of the barrel body (1).
3. A flow cytometer sample preparation cell filter as described in claim 1, wherein: the barrel (1) is internally provided with four square through holes, and the four mounting grooves (2) are respectively positioned on four surfaces of the square through holes.
4. A flow cytometer sample preparation cell filter as described in claim 1, wherein: the width of the rotating plate (4) is smaller than the depth of the mounting groove (2), and the length of the rotating plate (4) is larger than the distance between two opposite mounting grooves (2) in the barrel body (1).
5. A flow cytometer sample preparation cell filter as described in claim 1, wherein: the mounting hole has been seted up to the inside of rotor plate (4), the inside fixedly connected with filter screen (8) of mounting hole, the diameter of filter screen (8) is less than the diameter of centrifuging tube (7).
6. A flow cytometer sample preparation cell filter as described in claim 5, wherein: the inside swing joint of mounting hole has a feeding section of thick bamboo (9), the top fixedly connected with limiting plate (10) of a feeding section of thick bamboo (9), a feeding section of thick bamboo (9) swing joint is in the inside of barrel (1).
7. A flow cytometer sample preparation cell filter as described in claim 6, wherein: the top fixedly connected with locating plate (11) of barrel (1), limiting plate (10) swing joint is in the inside of locating plate (11), limiting plate (10) swing joint is in the top of barrel (1).
8. A flow cytometer sample preparation cell filter as described in claim 6, wherein: the top of limiting plate (10) is fixedly connected with feed inlet (12), feed inlet (12) are the toper setting.
9. A flow cytometer sample preparation cell filter as described in claim 5, wherein: the rotary plate (4) is provided with four, four the inside of rotary plate (4) all is provided with filter screen (8), four the filter screen interval of filter screen (8) is all inequality.
10. A flow cytometer sample preparation cell filter as described in claim 1, wherein: and magnetic blocks are arranged in the mounting groove (2) and on one side of the rotating plate (4) close to the mounting groove (2).
CN202120692607.6U 2021-04-06 2021-04-06 Cell filter for sample preparation of flow cytometer Active CN215050196U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202120692607.6U CN215050196U (en) 2021-04-06 2021-04-06 Cell filter for sample preparation of flow cytometer

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202120692607.6U CN215050196U (en) 2021-04-06 2021-04-06 Cell filter for sample preparation of flow cytometer

Publications (1)

Publication Number Publication Date
CN215050196U true CN215050196U (en) 2021-12-07

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ID=79149859

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Application Number Title Priority Date Filing Date
CN202120692607.6U Active CN215050196U (en) 2021-04-06 2021-04-06 Cell filter for sample preparation of flow cytometer

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114414459A (en) * 2022-01-02 2022-04-29 李志清 Sample intelligent efficient preparation system for flow cytometry and use method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114414459A (en) * 2022-01-02 2022-04-29 李志清 Sample intelligent efficient preparation system for flow cytometry and use method thereof
CN114414459B (en) * 2022-01-02 2024-02-13 西电集团医院 Sample intelligent efficient preparation system for flow cytometry and application method thereof

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Inventor after: Jia Xiaodong

Inventor after: Chen Baorong

Inventor after: Zhou Jianfeng

Inventor after: Chu Yulan

Inventor after: Xie Chunru

Inventor after: Li Xing

Inventor before: Chu Yulan

Inventor before: Zhou Jianfeng

Inventor before: Jia Xiaodong

Inventor before: Li Xing

Inventor before: Chen Baorong

CB03 Change of inventor or designer information