CN115717100A - Device for separating pathogenic bacteria from dried plant specimen and using method thereof - Google Patents
Device for separating pathogenic bacteria from dried plant specimen and using method thereof Download PDFInfo
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- CN115717100A CN115717100A CN202211381218.7A CN202211381218A CN115717100A CN 115717100 A CN115717100 A CN 115717100A CN 202211381218 A CN202211381218 A CN 202211381218A CN 115717100 A CN115717100 A CN 115717100A
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Abstract
The invention discloses a device for separating pathogenic bacteria from a dried plant specimen, which belongs to the technical field of bacteria separation, and comprises a containing barrel, wherein a support is arranged below the containing barrel, rotating blades are arranged in the containing barrel, and a driving assembly can repeatedly and continuously sieve bacteria with different sizes in a culture solution through different filter barrels and flow limiting barrels when working, so that each filter barrel can only filter the bacteria with the size close to the same size, the bacteria are separated step by step, the collected bacteria are discharged into a collecting box through a collecting group and a sealing group after being filtered, and then are discharged into a culture dish without manual participation, the steps of bacteria separation are simplified, the standardization of the bacteria separation can be improved through the separation, and the efficiency of the bacteria separation can be improved through the contact of the culture solution and the filter barrels in the process of quick rotation.
Description
Technical Field
The invention relates to the technical field of bacteria separation, in particular to a device for separating pathogenic bacteria from a plant dry specimen and a using method thereof.
Background
The bacterial culture has important effects on various aspects such as diagnosis, prevention, treatment, scientific research and the like of diseases, and the pathogenic diagnosis of infectious diseases needs to take bacterial specimens in a patient body for bacterial isolation culture, identification and drug sensitivity test, which is the most reliable basis for diagnosing infectious diseases and can also guide clinical treatment medication; the study of bacteriology to study the physiology, genetic variation, pathogenicity, immunity and drug resistance of bacteria requires artificial culture of bacteria, which is one of the prerequisites for human to find unknown new pathogenic bacteria.
At present, a common separation culture method is to manually perform necessary pretreatment on a sample, and then to perform streaking inoculation on several culture dishes by using an inoculator to perform separation culture so as to obtain bacterial colonies, further perform bacterial identification and drug sensitive detection, and separate bacteria with different sizes only by multiple steps, wherein the separation process needs to be performed by multiple steps, the separation efficiency is very low, the requirements of a laboratory cannot be met, the operation has considerable influence of human factors, and standardization operations are difficult to perform.
Based on the above, the invention designs a device for separating pathogenic bacteria from a dried plant specimen and a using method thereof, so as to solve the above problems.
Disclosure of Invention
The present invention is directed to a device for separating pathogenic bacteria from a dried plant specimen and a method for using the same, which solve the above problems of the prior art.
In order to achieve the purpose, the invention provides the following technical scheme: the device for separating pathogenic bacteria from the plant dry specimen comprises a containing barrel, wherein a support is arranged below the containing barrel, rotating blades are arranged inside the containing barrel, a plurality of fixing frames distributed in a circumferential array are fixedly connected to the outside of the containing barrel, the fixing frames are arranged in an anticlockwise and equidistant mode by taking the central point of the containing barrel as the center, the fixing frames are communicated with the inside of the containing barrel, a collection box for containing bacteria is connected to the inside of each fixing frame in a sliding mode, a first outlet and a second outlet are formed in the bottom of the collection box and the bottom of the fixing frame respectively, and a culture dish is arranged below each second outlet; the collecting boxes are fixedly connected with first springs for resetting the collecting boxes, the tops of a plurality of collecting boxes are fixedly connected with flow-limiting cylinders, the tops of the flow-limiting cylinders are rotatably connected with filter cylinders, and the flow-limiting cylinders are used for preventing bacteria from flowing out of the filter cylinders;
the sizes of the filter openings of the filter cartridges are different and gradually increased on the basis of the filter opening of the first filter cartridge; the first flow limiting cylinder is a solid cylinder body, a plurality of flow limiting cylinders are provided with filtering holes on the basis of the second flow limiting cylinder, and the size of each filtering hole is the same as that of a filtering opening of the previous filter cylinder; a plurality of flow-limiting cylinders are internally provided with a collection group, the collection group is used for collecting bacteria entering the interior of the filter cylinder, and a sealing group is arranged below the collection group; the sealing group is used for sealing the collecting box and releasing the sealing of the collecting box when the collecting group puts the collected bacteria into the collecting box; hold the bucket below and be provided with drive assembly, drive assembly is used for driving rotating vane to stir the inside culture solution of holding the bucket to push a plurality of collection box one by one and hold the bucket inside to the not equidimension bacterium separate step by step among the culture solution.
As a further scheme of the invention, the driving assembly comprises a motor, a tooth ring and an L-shaped rod; the motor is fixedly connected with the support through a fixing rod, an output shaft of the motor penetrates through the containing barrel to be fixedly connected with the rotating blades, the toothed ring is rotatably connected with the support, a gear is meshed with the toothed ring, the gear is fixedly connected with a first belt pulley, the first belt pulley is rotatably connected with the support, the output shaft of the motor is fixedly connected with a second belt pulley, and the first belt pulley and the second belt pulley are in transmission connection through a belt;
the L-shaped rod is connected to the outer side wall of the bracket in a sliding manner, the L-shaped rod is fixedly connected with a resisting sheet, the resisting sheet is provided with an inclined surface, and the resisting sheet is connected with a wedge block in a sliding manner; the wedge-shaped block is fixedly connected with a second spring for resetting the wedge-shaped block, the bottom of the wedge-shaped block is fixedly connected with a limiting rod, the limiting rod is provided with a sliding groove, the sliding groove is obliquely arranged, the sliding groove is connected with a connecting piece in a sliding manner, the top of the connecting piece is rotatably connected with a rotating piece, the top of the rotating piece is rotatably connected with a connecting rod, the connecting rod is rotatably connected with a fixing piece, the fixing piece is fixedly connected to the bottom of a supporting piece, the fixing piece is slidably connected with a first touch rod and a second touch rod, and the first touch rod and the second touch rod are located on two sides of a rotating shaft of the connecting rod; the connecting rod below is provided with the ejector pad, the ejector pad is located first touching pole and second touching pole intermediate position, ejector pad fixed connection is at tooth circle top.
As a further scheme of the invention, the collection group comprises a plurality of fan blades and a reciprocating screw rod, the fan blades are fixedly connected to the outside of the filter cartridge, the reciprocating screw rod is fixedly connected with the filter cartridge, the bottom of the reciprocating screw rod is positioned in the flow-limiting cylinder, the reciprocating screw rod is in threaded connection with a collection pipe, the collection pipe is in sliding connection with the flow-limiting cylinder, and the collection pipe is in sliding connection with a blocking piece.
As a further aspect of the present invention, the sealing set includes a sealing sheet, and the sealing sheet is slidably connected with the flow limiting cylinder; the sealing piece is fixedly connected with a third spring for resetting the sealing piece.
In a further aspect of the present invention, the sealing piece has a conical shape.
As a further scheme of the invention, the brackets are fixedly connected with placing frames which correspond to the fixed frames one by one; the rack is located fixed frame below and the equal fixed connection of its tail end holds the barrel head portion, the rack is used for placing the culture dish.
As a further aspect of the invention, the stent is ring-shaped.
As a further scheme of the invention, the bottom end of the bracket is fixedly connected with an anti-skid pad.
As a further aspect of the invention, the belt is a toothed belt.
As a further scheme of the invention, the bracket is provided with an annular groove, and the L-shaped rod is connected with the annular groove in a sliding manner.
A method for isolating pathogenic bacteria from a dried plant specimen, the method comprising the steps of:
the method comprises the following steps: the driving assembly drives a plurality of filter cartridges with different filter openings and flow limiting cartridges with different filter holes to screen and filter bacteria in the culture solution one by one so as to realize gradual filtration of the bacteria;
step two: collecting the bacteria filtered by the filter cartridge and the flow-limiting cartridge through a collection group;
step three: the collection group discharges the bacteria into the collection box through the sealing group after the bacteria are collected, and the bacteria are discharged after being collected for a plurality of times;
step four: will collect the box through supporting piece and wedge and promote to holding bucket inside to make and collect the box and stay among holding the bucket in the time of ejector pad rotation a week.
Compared with the prior art, the invention has the beneficial effects that:
drive assembly during operation can carry out repeated screening constantly to the bacterium of equidimension among the culture solution through different cartridge filter and current-limiting cylinder, make each cartridge filter can only be close the same to the bacterium size and filter, realize the separation step by step of bacterium, discharge the bacterium of accomplishing through collection group and sealed group after filtering and collect among the box, then among discharging the culture dish, do not need artifical the participation can carry out automatic collection and separation, the step of bacterium separation has been simplified, and can improve the standardization of bacterium separation through this mode separation, the efficiency that the culture solution can improve the bacterium separation with the cartridge filter contact on the way of quick rotation.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention;
fig. 2 is an internal view of the tub according to the present invention;
FIG. 3 is a block diagram of the carrier and gears of the present invention;
fig. 4 is a structural view of a first touch bar and a second touch bar of the present invention;
FIG. 5 is a diagram showing the positional relationship between the rotating member and the connecting rod according to the present invention;
FIG. 6 is a diagram of the positioning of the collection cartridge and filter cartridge of the present invention;
FIG. 7 is a cross-sectional view of a collection cartridge and filter cartridge in accordance with the present invention;
FIG. 8 is a cross-sectional view of a flow restrictor cartridge of the present invention;
FIG. 9 is a cross-sectional view of a filter tube according to the present invention;
FIG. 10 is a schematic diagram of the cartridge and biological filter membrane of the present invention;
FIG. 11 is a flow chart of the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
1. a containing barrel; 2. a rotating blade; 3. a fixing frame; 4. a collection box; 5. a flow-limiting barrel; 6. a filter cartridge; 7. a support; 8. a first outlet; 9. a second outlet; 10. a motor; 11. fixing the rod; 12. a tooth ring; 13. a gear; 14. a first pulley; 15. a second pulley; 16. a belt; 17. an L-shaped rod; 18. pressing the slices; 19. a wedge block; 20. a second spring; 21. a limiting rod; 22. a connecting member; 23. a connecting rod; 24. a fixing sheet; 25. a first touch lever; 26. a second touch lever; 27. pushing a block; 28. a fan blade; 29. a reciprocating screw rod; 30. a collection pipe; 31. a baffle plate; 32. sealing the sheet; 33. a third spring; 34. placing a rack; 35. a non-slip mat; 36. an annular groove; 37. a first spring; 38. a chute; 39. a rotating member; 40. a cylinder body; 41. biological filtration membranes.
Detailed Description
Referring to fig. 1-11, the present invention provides a technical solution: the device for separating pathogenic bacteria from the dried plant specimen comprises a containing barrel 1, wherein a support 7 is arranged below the containing barrel 1, a rotating blade 2 is arranged inside the containing barrel 1, a plurality of fixing frames 3 are fixedly connected to the outside of the containing barrel 1, the fixing frames 3 are arranged in an anticlockwise and equidistant mode by taking the central point of the containing barrel 1 as the center, the fixing frames 3 are all communicated with the inside of the containing barrel 1, a collection box 4 for containing bacteria is connected inside the fixing frames 3 in a sliding mode, a first outlet 8 and a second outlet 9 are respectively formed in the bottom of the collection box 4 and the bottom of the fixing frames 3, and a culture dish is arranged below the second outlet 9; the collection boxes 4 are fixedly connected with first springs 37 for resetting the collection boxes, the tops of the collection boxes 4 are fixedly connected with flow-limiting cylinders 5, the tops of the flow-limiting cylinders 5 are rotatably connected with filter cylinders 6, and the flow-limiting cylinders 5 are used for preventing bacteria from flowing out of the filter cylinders 6;
the sizes of the filter openings of the plurality of filter cartridges 6 are different, and the sizes of the filter openings are gradually increased on the basis of the filter opening of the first filter cartridge 6; the first flow limiting cylinder 5 is a solid cylinder, a plurality of flow limiting cylinders 5 are provided with filtering holes on the basis of the second flow limiting cylinder, and the size of each filtering hole is the same as that of the filtering opening of the previous filter cylinder 6; a collection group is arranged in each of the plurality of flow-limiting cylinders 5 and is used for collecting bacteria entering the interior of the filter cylinder 6, and a sealing group is arranged below the collection group; the sealing group is used for sealing the collection box 4 and releasing the sealing of the collection box 4 when the collection group puts the collected bacteria into the collection box 4; hold 1 below of bucket and be provided with drive assembly, drive assembly is used for driving rotating vane 2 to stir the culture solution that holds 1 inside of bucket and push a plurality of collection box 4 one by one and hold 1 inside bacterium to equidimension among the culture solution and separate step by step.
When the scheme is put into practical use, the filter cylinder 6 and the flow limiting cylinder 5 both consist of a cylinder body 40 and a biological filtering membrane 41, the cylinder body 40 is hollow, the biological filtering membrane 41 is bonded with the cylinder body 40 in a waterproof adhesive tape mode and the like, so that the biological filtering membrane 42 can be conveniently replaced and detached, the containing barrel 1 is supported by the support 7, the bacteria culture solution is poured into the containing barrel 1, then the driving assembly is started, the driving assembly drives the rotating blades 2 to rotate inside the containing barrel 1 when working, the rotating blades 2 can form the culture solution into a vortex shape when rotating, so that the culture solution rotates along the inner wall of the containing barrel 1, the driving assembly can extrude the first collecting box 4, the first collecting box 4 can stay for a period of time inside the containing barrel 1, after the first collecting box 4 enters the containing barrel 1, the vortex-shaped rotating culture solution can continuously flush the filter cylinder 6, the filter cylinder 6 can block larger bacteria from the outside the filter cylinder 6, so that the smaller bacteria can enter the filter cylinder 6, and the smaller flow limiting cylinder 5 can prevent most of the smaller bacteria from flowing out of the filter cylinder, and the smaller flow limiting cylinder 5 can prevent the smaller bacteria from flowing out of the filter cylinder; during the period of time when the first filter cylinder 6 filters the culture solution, the collection group collects the small bacteria staying inside the flow limiting cylinder 5 for multiple times, each time the collection group collects the bacteria inside the flow limiting cylinder 5 and then descends along the flow limiting cylinder 5, and each time the collection group descends to the lowest part of the flow limiting cylinder 5, the sealing group releases the sealing of the collection box 4, so that the small bacteria collected by the collection group are discharged into the collection box 4;
the filter cartridge 6 continuously filters smaller bacteria in the culture solution during the time that the first collection cartridge 4 stays inside the tub 1, and the collection group continuously collects bacteria filtered by the filter cartridge 6 during the time that the filter cartridge 6 takes care of the bacteria, and then discharges the collected smaller bacteria into the collection cartridge 4;
after the period of time that the first collecting box 4 stays inside the containing barrel 1 is over, the driving assembly can extrude the second collecting box 4, so that the second collecting box 4 can also stay inside the containing barrel 1 for a period of time, when the driving assembly extrudes the second collecting box 4 to the inside of the containing barrel 1, the previous collecting box 4 can automatically reset to an initial position from the inside of the containing barrel 1 under the action of the first spring 37, so that the first filtering barrel 6 stops filtering bacteria, the first outlet 8 and the second outlet 9 are overlapped after the first collecting box 4 resets, and at the moment, the bacteria collected by the first collecting box 4 are discharged into a culture dish below the fixing frame 3 through the first outlet 8 and the second outlet 9; because the filter opening of the second filter cartridge 6 is larger than the filter opening of the previous filter cartridge 6, when the second collection cartridge 4 enters the containing barrel 1, the filter cartridge 6 above the second collection cartridge 4 can filter slightly larger bacteria than the first filter cartridge 6, and the second flow-limiting cartridge 5 is provided with filter holes, and the filter holes formed in the second flow-limiting cartridge 5 are the same as the filter opening of the previous filter cartridge 6; the second filter cylinder 6 blocks bacteria larger than the filter opening of the second filter cylinder from the outside, and bacteria smaller than and equal to the filter opening of the second filter cylinder enter the inside of the flow-limiting cylinder 5, while bacteria smaller than the filter opening of the second flow-limiting cylinder 5 flow out of the inside of the flow-limiting cylinder 5, so that only some bacteria with nearly the same size can be left in the inside of the second flow-limiting cylinder 5, and the collection group and the sealing group can collect the bacteria with nearly the same size into the second collection box 4;
the bacteria smaller than the filtering port of the second filter cylinder 6 can be automatically discharged through the filtering holes formed in the second flow limiting cylinder 5, only the bacteria with the same size and larger than the bacteria filtered by the first filter cylinder 6 are left, and the standardization and the separation efficiency of bacteria separation are improved.
After the period of time that the second collecting box 4 stays inside the tub 1 is over, the driving assembly may press the third collecting box 4, so that the third collecting box 4 can also stay inside the tub 1 for a period of time.
The bacteria with different sizes in the culture solution can be repeatedly and continuously screened through different filter cartridges 6 and flow limiting cylinders 5, so that each filter cartridge 6 can only filter the bacteria with the size close to the same size, the bacteria can be separated step by step, the collected bacteria are discharged into the collecting box 4 through the collecting group and the sealing group after being filtered, and then the bacteria are discharged into a culture dish, the bacteria can be automatically collected and separated without manual participation, the bacteria separation steps are simplified, the bacteria separation standardization can be improved through the separation, and the efficiency of the bacteria separation can be improved by the culture solution in contact with the filter cartridges 6 on the way of rapid rotation.
As a further aspect of the present invention, the driving assembly includes a motor 10, a ring gear 12 and an L-shaped rod 17; the motor 10 is fixedly connected with the support 7 through a fixing rod 11, an output shaft of the motor 10 penetrates through the containing barrel 1 to be fixedly connected with the rotating blades 2, the toothed ring 12 is rotatably connected with the support 7, the toothed ring 12 is meshed with a gear 13, the gear 13 is fixedly connected with a first belt pulley 14, the first belt pulley 14 is rotatably connected with the support 7, the output shaft of the motor 10 is fixedly connected with a second belt pulley 15, and the first belt pulley 14 is in transmission connection with the second belt pulley 15 through a belt 16;
the L-shaped rod 17 is connected to the outer side wall of the bracket 7 in a sliding manner, the L-shaped rod 17 is fixedly connected with a propping piece 18, the propping piece 18 is provided with an inclined surface, and the propping piece 18 is connected with a wedge block 19 in a sliding manner; the wedge-shaped block 19 is fixedly connected with a second spring 20 for resetting the wedge-shaped block, the bottom of the wedge-shaped block 19 is fixedly connected with a limiting rod 21, the limiting rod 21 is provided with a sliding groove 38, the sliding groove 38 is obliquely arranged, the sliding groove 38 is slidably connected with a connecting piece 22, the top of the connecting piece 22 is rotatably connected with a rotating piece 39, the top of the rotating piece 39 is slidably connected with a connecting rod 23, the connecting rod 23 is rotatably connected with a fixing piece 24, the fixing piece 24 is fixedly connected to the bottom of the supporting piece 18, the fixing piece 24 is slidably connected with a first touch rod 25 and a second touch rod 26, and the first touch rod 25 and the second touch rod 26 are positioned on two sides of the rotating shaft of the connecting rod 23; a push block 27 is arranged below the connecting rod 23, the push block 27 is positioned between the first touch rod 25 and the second touch rod 26, and the push block 27 is fixedly connected to the top of the tooth ring 12.
When the driving assembly works, the motor 10 is started, an output shaft of the motor 10 drives the rotating blade 2 to stir culture solution in the containing barrel 1, the motor 10 drives the second belt pulley 15 to rotate when rotating, the second belt pulley 15 drives the first belt pulley 14 and the gear 13 to rotate through the belt 16 when rotating, the gear 13 drives the toothed ring 12 to rotate at the top of the support 7 when rotating, the toothed ring 12 drives the push block 27 to rotate when rotating, the push block 27 pushes the first touch rod 25 to enable the L-shaped rod 17 and the resisting sheet 18 to slide around the support 7 when rotating, the resisting sheet 18 can be in contact with the outer part of the collecting box 4 when the L-shaped rod 17 slides around the support 7, and the end part of the collecting box 4 can gradually rise along the inclined plane formed when the resisting sheet 18 rotates, so that the collecting box 4 is pushed inwards;
when the collecting box 4 enters the containing barrel 1, the plane of the resisting sheet 18 contacts with the end part of the collecting box 4, but the L-shaped rod 17 and the resisting sheet 18 continuously rotate at the moment, the end part of the collecting box 4 can extrude the wedge-shaped block 19 along with the continuous rotation of the resisting sheet 18 and the L-shaped rod 17, the wedge-shaped block 19 can compress the second spring 20 when being extruded, the wedge-shaped block 19 can drive the limiting rod 21 to move towards the direction far away from the collecting box 4 when being extruded, the connecting piece 22 slides in the chute 38 when the limiting rod 21 moves, the connecting piece 22 can drive the connecting rod 23 to rotate when sliding, the rotating piece 39 rotates along the connecting rod 23 when the connecting rod 23 rotates, the connecting rod 23 can drive the first touch rod 25 to slide and rise along the fixing piece 24 and the second touch rod 26 to slide and fall along the fixing piece 24 when rotating; the first touch rod 25 is separated from the push block 27 after rising, at the moment, the tooth ring 12 drives the push block 27 to rotate, so that the L-shaped rod 17 and the abutting piece 18 stay at the position, the collection box 4 can stay in the containing barrel 1 for a period of time, bacteria in the culture solution are screened through the filter barrel 6 and the flow limiting barrel 5, after the tooth ring 12 drives the push block 27 to rotate for a circle, the push block 27 is contacted with the second touch rod 26 and pushes the L-shaped rod 17 and the abutting piece 18 to rotate through the second touch rod 26, along with the rotation of the abutting piece 18 and the L-shaped rod 17, the wedge block 19 is separated from the outer end of the collection box 4, the collection box 4 automatically resets under the action of the first spring 37, the second spring 20 also drives the wedge block 19 to reset, and the connecting rod 23 resets after the wedge block 19 resets, so that the first touch rod 25 is at a low position and the second touch rod 26 is at a high position;
As a further scheme of the invention, the collection set comprises a plurality of fan blades 28 and a reciprocating screw rod 29, the fan blades 28 are fixedly connected to the outside of the filter cartridge 6, the reciprocating screw rod 29 is fixedly connected with the filter cartridge 6, the bottom of the reciprocating screw rod 29 is positioned in the flow-limiting cylinder 5, the reciprocating screw rod 29 is in threaded connection with a collection pipe 30, the collection pipe 30 is in sliding connection with the flow-limiting cylinder 5, and the collection pipe 30 is in sliding connection with a baffle 31.
When the collecting group works, after the collecting box 4 enters the containing barrel 1, the culture solution drives the filter cylinder 6 to rotate through the plurality of fan blades 28 because the culture solution is in a rotating state, the filter cylinder 6 drives the reciprocating screw rod 29 to rotate when rotating, the reciprocating screw rod 29 drives the collecting pipe 30 to reciprocate, when the reciprocating screw rod 29 descends, the baffle sheet 31 can slide upwards under the lifting of water flow, so that the top of the collecting pipe 30 is sealed, the collecting pipe 30 can drive bacteria in the flow limiting cylinder 5 to move downwards when descending, and when the collecting pipe 30 descends to the lowest position, the sealing group can relieve the sealing of the collecting box 4, so that the bacteria in the collecting pipe 30 enter the collecting box 4; when the collecting pipe 30 ascends, the baffle plate 31 slides downwards, bacteria in the flow restricting cylinder 5 enter below the collecting pipe 30, when the collecting pipe 30 descends again, the bacteria in the flow restricting cylinder 5 are collected again and then discharged into the collecting box 4 again, and when the collecting box 4 is reset, the first outlet 8 and the second outlet 9 are overlapped, so that the bacteria collected in the collecting box 4 flow into the culture dish.
As a further aspect of the present invention, the sealing set includes a sealing sheet 32, and the sealing sheet 32 is slidably connected to the flow-limiting cylinder 5; a third spring 33 for returning the sealing piece 32 is fixedly connected to the sealing piece.
Above-mentioned sealed group is at the during operation, when collecting pipe 30 descends to the lowest, the bottom of collecting pipe 30 can push down the sealing strip 32 to collect inside the box 4, the sealing strip 32 can compress third spring 33 when the sealing strip descends, the sealing strip 32 can contact when descending to collect inside the box 4 and to collecting the sealed of box 4 to the bacterium that makes collecting pipe 30 collect and a small amount of culture solution directly discharge when the collecting pipe 30 rises among collecting box 4, third spring 33 drives the sealing strip 32 and resets and continue to seal collecting box 4.
In a further aspect of the present invention, the sealing piece 32 has a conical shape.
When the scheme works, the sealing sheet 32 is conical, so that when the sealing sheet 32 enters the collecting box 4, bacteria collected by the collecting pipe 30 can descend along the sealing sheet 32, and the phenomenon that excessive bacteria cannot be discharged above the sealing sheet 32 is prevented.
As a further scheme of the present invention, the support 7 is fixedly connected with placing frames 34 corresponding to the fixing frames 3 one by one; the rack 34 is located fixed frame 3 below and the equal fixed connection of its tail end holds 1 bottom of bucket, the rack 34 is used for placing the culture dish.
Above-mentioned scheme can place the culture dish through rack 34 at the during operation, conveniently gets to take to can also support motor 10.
As a further aspect of the invention, the support 7 is ring-shaped.
Above-mentioned scheme is at the during operation, and support 7 is the annular, makes things convenient for tooth circle 12 to slide at the top of support 7.
As a further scheme of the invention, the bottom end of the bracket 7 is fixedly connected with a non-slip mat 35.
Above-mentioned scheme is at the during operation, can increase the steadiness of support 7 through slipmat 35.
As a further aspect of the invention, the belt 16 is a toothed belt.
When the scheme works, the friction force of the toothed belt is large, the second belt pulley 15 and the first belt pulley 14 can be driven by the belt 16, and the sliding phenomenon cannot occur.
As a further aspect of the present invention, the bracket 7 is provided with an annular groove 36, and the L-shaped rod 17 is slidably connected to the annular groove 36.
When the scheme works, the L-shaped rod 17 can be limited through the annular groove 36 when the L-shaped rod 17 slides along the annular groove 36.
A method for isolating pathogenic bacteria from a dried vegetative specimen, comprising the steps of:
the method comprises the following steps: the driving component drives a plurality of filter cartridges 6 with different filter openings and flow limiting cartridges 5 with different filter holes to screen and filter bacteria in the culture solution one by one to realize the step-by-step filtration of the bacteria;
step two: collecting the bacteria filtered by the filter cartridge 6 and the flow-limiting cartridge 5 through a collection group;
step three: the collection group discharges the bacteria into the collection box 4 through the sealing group after the bacteria are collected, and the bacteria are discharged after being collected for a plurality of times;
step four: the collection box 4 is pushed inside the tub 1 by the stopper piece 18 and the wedge 19, and the collection box 4 is made to stay in the tub 1 for a period of one rotation of the stopper 27.
The working principle is as follows: the containing barrel 1 is supported through the support 7, the bacteria culture solution is poured into the containing barrel 1, then the driving assembly is started, the driving assembly can drive the rotating blades 2 to rotate inside the containing barrel 1 when working, the rotating blades 2 can form the culture solution into a vortex shape when rotating, the culture solution can rotate along the inner wall of the containing barrel 1, the driving assembly can extrude the first collecting box 4, the first collecting box 4 can stay inside the containing barrel 1 for a period of time, after the first collecting box 4 enters the containing barrel 1, the culture solution rotating in the vortex shape can continuously wash the filter barrel 6, larger bacteria can be blocked outside the filter barrel 6 through the filter barrel 6, smaller bacteria can enter the filter barrel 6, the smaller bacteria can be limited under the action of the flow limiting barrel 5 due to the fact that the first flow limiting barrel 5 is an entity, the smaller bacteria can be prevented from flowing out along with the rotation of the culture solution from the inside the filter barrel 6, and most of the smaller bacteria can stay inside the flow limiting barrel 5; during the period of time when the first filter cylinder 6 filters the culture solution, the collection group collects the small bacteria staying inside the flow limiting cylinder 5 for multiple times, each time the collection group collects the bacteria inside the flow limiting cylinder 5 and then descends along the flow limiting cylinder 5, and each time the collection group descends to the lowest part of the flow limiting cylinder 5, the sealing group releases the sealing of the collection box 4, so that the small bacteria collected by the collection group are discharged into the collection box 4;
the filter cartridge 6 continuously filters smaller bacteria in the culture solution during the time that the first collection cartridge 4 stays inside the tub 1, and the collection group continuously collects bacteria filtered by the filter cartridge 6 during the time that the filter cartridge 6 takes care of the bacteria, and then discharges the collected smaller bacteria into the collection cartridge 4;
after the period of time that the first collecting box 4 stays inside the containing barrel 1 is over, the driving assembly can extrude the second collecting box 4, so that the second collecting box 4 can also stay inside the containing barrel 1 for a period of time, when the driving assembly extrudes the second collecting box 4 to the inside of the containing barrel 1, the previous collecting box 4 can automatically reset to an initial position from the inside of the containing barrel 1 under the action of the first spring 37, so that the first filtering barrel 6 stops filtering bacteria, the first outlet 8 and the second outlet 9 are overlapped after the first collecting box 4 resets, and at the moment, the bacteria collected by the first collecting box 4 are discharged into a culture dish below the fixing frame 3 through the first outlet 8 and the second outlet 9; because the filter opening of the second filter cartridge 6 is larger than the filter opening of the previous filter cartridge 6, when the second collection cartridge 4 enters the containing barrel 1, the filter cartridge 6 above the second collection cartridge 4 can filter slightly larger bacteria than the first filter cartridge 6, the second flow-limiting cartridge 5 is provided with filter holes, and the filter holes formed in the second flow-limiting cartridge 5 are the same as the filter opening of the previous filter cartridge 6; the second filter cylinder 6 blocks bacteria larger than the filter opening of the second filter cylinder from the outside, and bacteria smaller than and equal to the filter opening of the second filter cylinder enter the inside of the flow-limiting cylinder 5, while bacteria smaller than the filter opening of the second flow-limiting cylinder 5 flow out of the inside of the flow-limiting cylinder 5, so that only some bacteria with nearly the same size can be left in the inside of the second flow-limiting cylinder 5, and the collection group and the sealing group can collect the bacteria with nearly the same size into the second collection box 4;
the bacteria smaller than the filtering port of the second filter cylinder 6 can be automatically discharged through the filtering holes formed in the second flow limiting cylinder 5, only the bacteria with the same size and larger than the bacteria filtered by the first filter cylinder 6 are left, and the standardization and the separation efficiency of bacteria separation are improved.
After the second collecting box 4 stays inside the tub 1 for the period of time, the driving assembly presses the third collecting box 4, so that the third collecting box 4 can stay inside the tub 1 for the period of time.
Can sieve repeatedly through filter cylinder 6 and current-limiting cylinder 5 of difference not equidimension bacterium among the culture solution for each filter cylinder 6 can only be close the same to the bacterium size filters, realize the separation step by step of bacterium, discharge the bacterium of collecting the completion through collection group and seal group after filtering and collect among the box 4, then discharge among the culture dish, do not need artifical the participation can carry out automatic collection and separation, the step of bacterium separation has been simplified, and can improve the standardization of bacterium separation through this mode separation, the culture solution can improve the efficiency of bacterium separation with filter cylinder 6 contact on the way of quick rotation.
Claims (11)
1. The utility model provides a device of separation pathogenic bacterium in dry sample of plant, is including holding bucket (1), it is provided with support (7), its characterized in that to hold bucket (1) below: the bacteria-containing pot is characterized in that a rotating blade (2) is arranged inside the containing barrel (1), a plurality of fixed frames (3) distributed in a circumferential array manner are fixedly connected to the outside of the containing barrel (1), the plurality of fixed frames (3) are all communicated with the inside of the containing barrel (1), a collection box (4) for containing bacteria is all slidably connected to the inside of the plurality of fixed frames (3), a first outlet (8) and a second outlet (9) are respectively formed in the bottom of the collection box (4) and the bottom of the fixed frames (3), and a culture dish is arranged below the second outlet (9); the collection boxes (4) are fixedly connected with first springs (37) for resetting the collection boxes, the tops of the collection boxes (4) are fixedly connected with flow limiting cylinders (5), the tops of the flow limiting cylinders (5) are rotatably connected with filter cylinders (6), and the flow limiting cylinders (5) are used for preventing bacteria from flowing out of the filter cylinders (6);
the sizes of the filter openings of the filter cartridges (6) are different and gradually increased on the basis of the filter opening of the first filter cartridge (6); the first flow limiting cylinder (5) is a solid cylinder, a plurality of flow limiting cylinders (5) are provided with filtering holes on the basis of the second flow limiting cylinder, and the size of each filtering hole is the same as that of the filtering opening of the previous filter cylinder (6); a collection group is arranged in each of the plurality of flow limiting cylinders (5), the collection groups are used for collecting bacteria entering the interior of the filter cylinder (6), and a sealing group is arranged below each collection group; the sealing group is used for sealing the collection box (4) and releasing the sealing of the collection box (4) when the collection group puts the collected bacteria into the collection box (4); hold bucket (1) below and be provided with drive assembly, drive assembly is used for driving rotating vane (2) to stir the culture solution that holds bucket (1) inside to with a plurality of collection box (4) push into one by one and hold bucket (1) inside to the bacterium of equidimension not separate step by step among the culture solution.
2. The apparatus for isolating pathogenic bacteria from a dried plant specimen according to claim 1, wherein: the driving component comprises a motor (10), a tooth ring (12) and an L-shaped rod (17); the motor (10) is fixedly connected with the support (7) through a fixing rod (11), an output shaft of the motor (10) penetrates through the containing barrel (1) and is fixedly connected with the rotating blades (2), the toothed ring (12) is rotatably connected with the support (7), the toothed ring (12) is meshed with a gear (13), the gear (13) is fixedly connected with a first belt pulley (14), the first belt pulley (14) is rotatably connected with the support (7), an output shaft of the motor (10) is fixedly connected with a second belt pulley (15), and the first belt pulley (14) is in transmission connection with the second belt pulley (15) through a belt (16);
the L-shaped rod (17) is connected to the outer side wall of the support (7) in a sliding mode, the L-shaped rod (17) is fixedly connected with a propping piece (18), the propping piece (18) is provided with an inclined surface, and the propping piece (18) is connected with a wedge block (19) in a sliding mode; the wedge block (19) is fixedly connected with a second spring (20) for resetting the wedge block, the bottom of the wedge block (19) is fixedly connected with a limiting rod (21), a sliding groove (38) is formed in the limiting rod (21), the sliding groove (38) is obliquely arranged, a connecting piece (22) is slidably connected with the sliding groove (38), a rotating piece (39) is rotatably connected to the top of the connecting piece (22), a connecting rod (23) is slidably connected to the top of the rotating piece (39), the connecting rod (23) is rotatably connected with a fixing piece (24), the fixing piece (24) is fixedly connected to the bottom of the supporting piece (18), the fixing piece (24) is slidably connected with a first touch rod (25) and a second touch rod (26), and the first touch rod (25) and the second touch rod (26) are located on two sides of the rotating shaft of the connecting rod (23); connecting rod (23) below is provided with ejector pad (27), ejector pad (27) are located first touch pole (25) and second touch pole (26) intermediate position, ejector pad (27) fixed connection is at tooth circle (12) top.
3. The apparatus for isolating pathogenic bacteria from a dried plant specimen according to claim 1, wherein: the collecting set comprises a plurality of fan blades (28) and a reciprocating screw rod (29), the fan blades (28) are fixedly connected to the outside of the filter cartridge (6), the reciprocating screw rod (29) is fixedly connected with the filter cartridge (6), the bottom of the reciprocating screw rod (29) is located inside the flow limiting cylinder (5), the reciprocating screw rod (29) is in threaded connection with a collecting pipe (30), the collecting pipe (30) is in sliding connection with the flow limiting cylinder (5), and the collecting pipe (30) is in sliding connection with a blocking piece (31).
4. The apparatus for isolating pathogenic bacteria from a dried plant specimen according to claim 1, wherein: the sealing group comprises a sealing sheet (32), and the sealing sheet (32) is in sliding connection with the flow limiting cylinder (5); and a third spring (33) for resetting the sealing piece (32) is fixedly connected with the sealing piece.
5. The apparatus for isolating pathogenic bacteria from a dried plant specimen according to claim 4, wherein: the sealing piece (32) is conical.
6. The apparatus for isolating pathogenic bacteria from a dried plant specimen according to claim 1, wherein: the bracket (7) is fixedly connected with placing racks (34) which correspond to the fixed frames (3) one by one; the rack (34) is located below the fixing frame (3), the tail end of the rack is fixedly connected to the bottom of the containing barrel (1), and the rack (34) is used for containing a culture dish.
7. The apparatus for isolating pathogenic bacteria from a dried plant specimen according to claim 1, wherein: the support (7) is annular.
8. The apparatus for isolating pathogenic bacteria from a dried plant specimen according to claim 1, wherein: the bottom end of the bracket (7) is fixedly connected with an anti-skid pad (35).
9. The apparatus for isolating pathogenic bacteria from a dried plant specimen according to claim 2, wherein: the belt (16) is a toothed belt.
10. The apparatus for isolating pathogenic bacteria from a dried plant specimen according to claim 2, wherein: annular groove (36) have been seted up in support (7), L type pole (17) and annular groove (36) sliding connection.
11. A method for isolating pathogenic bacteria from a dried vegetative specimen, suitable for use in a device for isolating pathogenic bacteria from a dried vegetative specimen according to any one of claims 1 to 10, comprising the steps of:
the method comprises the following steps: the driving component drives a plurality of filter cartridges (6) with different sizes of filter orifices and flow limiting cartridges (5) with different sizes of filter holes to screen and filter bacteria in the culture solution one by one to realize the gradual filtration of the bacteria;
step two: collecting bacteria filtered by the filter cartridge (6) and the flow limiting cylinder (5) through a collecting group;
step three: the collection group discharges the bacteria into the collection box (4) through the sealing group after the bacteria are collected, and the bacteria are discharged after being collected for a plurality of times;
step four: the collecting box (4) is pushed to the inside of the containing barrel (1) through the abutting sheet (18) and the wedge-shaped block (19), and the collecting box (4) stays in the containing barrel (1) within the time of one rotation of the pushing block (27).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211381218.7A CN115717100A (en) | 2022-11-06 | 2022-11-06 | Device for separating pathogenic bacteria from dried plant specimen and using method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211381218.7A CN115717100A (en) | 2022-11-06 | 2022-11-06 | Device for separating pathogenic bacteria from dried plant specimen and using method thereof |
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| CN115717100A true CN115717100A (en) | 2023-02-28 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117298800A (en) * | 2023-11-27 | 2023-12-29 | 山西亚鑫新能科技有限公司 | VOCs collection and waste gas comprehensive treatment system and method |
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2022
- 2022-11-06 CN CN202211381218.7A patent/CN115717100A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117298800A (en) * | 2023-11-27 | 2023-12-29 | 山西亚鑫新能科技有限公司 | VOCs collection and waste gas comprehensive treatment system and method |
| CN117298800B (en) * | 2023-11-27 | 2024-02-06 | 山西亚鑫新能科技有限公司 | VOCs collection and waste gas comprehensive treatment system and method |
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