CN113176123A - Experimental container and biological sample preparation device - Google Patents
Experimental container and biological sample preparation device Download PDFInfo
- Publication number
- CN113176123A CN113176123A CN202110473378.3A CN202110473378A CN113176123A CN 113176123 A CN113176123 A CN 113176123A CN 202110473378 A CN202110473378 A CN 202110473378A CN 113176123 A CN113176123 A CN 113176123A
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- teeth
- cover
- row
- guide tube
- container
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2866—Grinding or homogeneising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2873—Cutting or cleaving
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
An embodiment of the present invention provides an experiment container, including: a container body; a first cover; and a processing assembly disposed on the first cover; the processing assembly comprises a guide pipe which passes through the first cover and can rotate relative to the first cover, at least one row of teeth which are fixed on the first cover and arranged around the guide pipe, and an agitating element which is fixed on the guide pipe; the stirring element includes at least one cantilevered cutting blade and a structural member cooperating with the at least one row of teeth, the structural member extending from the guide tube and overlying the at least one row of teeth. According to the embodiment of the invention, the tissue put into the experimental container is cut by the rotatable cantilever type cutting knife, the tissue put into the experimental container is ground by the plurality of rows of teeth fixed on the first cover and the rotatable structural member covered on the first cover, and when the cantilever type cutting knife and the structural member rotate, the tissue in the experimental container is repeatedly cut and ground, so that cells in the prepared biological sample are more fully dispersed.
Description
Technical Field
The invention relates to the technical field of experimental instruments, in particular to an experimental container and a biological sample preparation device.
Background
Tissue is the cellular architecture that borders between cells and organs, and is composed of many morphologically similar cells and intercellular substances. For the cellular study of organisms, tissues need to be processed to prepare single cell suspensions. A single cell suspension is a biological sample that releases discrete individual cells as the connections between cells in the tissue are broken. The prepared single cell suspension has the characteristics of sufficient cell dispersion, high activity and the like, namely, the connection between cells needs to be fully destroyed, and the integrity and the activity of a single cell are kept, so that the single cell suspension is the basis for cell research.
Based on the above characteristics of single-cell suspensions, higher requirements are placed on the structure of the container and/or the device for preparing single-cell suspensions. When the existing device is used for treating tissues with strong adhesion, such as mouse brain tissues, the cell dispersibility in the prepared single cell suspension is not ideal.
Disclosure of Invention
The embodiment of the invention provides an experimental container, aiming at solving the problem of insufficient cell dispersion in a single cell suspension in the prior art.
In a first aspect, there is provided an assay container comprising:
a container body (1) having an open end (11) and a closed end (12);
a first lid (2) connected to the open end (11) of the container body (1); and
a processing assembly (3) arranged on the first cover (2);
wherein the processing assembly (3) comprises a guide tube (31) which passes through the first cover (2) and is rotatable relative to the first cover (2), at least one row of teeth (32) which are fixed on the first cover (2) and arranged around the guide tube (31), and a stirring element (33) which is fixed on the guide tube (31);
wherein the stirring element (33) comprises at least one cantilever cutting knife (331) and a structural member (332) cooperating with the at least one row of teeth (32), the structural member (332) extending from the guide tube (31) and covering the at least one row of teeth (32).
In a second aspect, there is provided a biological sample preparation device comprising a housing, a motor and an assay container as described above.
According to the embodiment of the invention, the tissue put into the experimental container is cut by the rotatable cantilever type cutting knife, the tissue put into the experimental container is ground by the plurality of rows of teeth fixed on the first cover and the rotatable structural member covered on the first cover, and when the cantilever type cutting knife and the structural member rotate, the tissue in the experimental container is repeatedly cut and ground, so that cells in the prepared biological sample are more fully dispersed.
Drawings
The foregoing and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
FIG. 1 is an exploded view of an experimental vessel according to a first embodiment of the present invention;
fig. 2 is a side view of the first cover 2 and the processing assembly 3 according to the second embodiment of the present invention;
fig. 3 is a plan view of the first cover 2 and the processing assembly 3 according to the second embodiment of the present invention;
FIG. 4 is a schematic view of region B of FIG. 3;
fig. 5 is a schematic structural diagram of a biological sample preparation device according to a third embodiment of the present invention.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar modules or modules having the same or similar functionality throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention. On the contrary, the embodiments of the invention include all changes, modifications and equivalents coming within the spirit and terms of the claims appended hereto.
According to the embodiment of the invention, the tissue put into the experimental container is cut by the rotatable cantilever type cutting knife, the tissue put into the experimental container is ground by the plurality of rows of teeth fixed on the first cover and the rotatable structural member covered on the first cover, and when the cantilever type cutting knife and the structural member rotate, the tissue in the experimental container is repeatedly cut and ground, so that cells in the prepared biological sample are more fully dispersed.
Example one
Fig. 1 is an exploded view of an experimental container according to a first embodiment of the present invention. As shown in fig. 1, the test container is shaped like a test tube and includes a container body 1, a first cap 2, and a processing assembly 3. The container body 1 has an open end 11 and a closed end 12. The outer surface of the opening end 11 is provided with external threads in a partial area, and the external threads can be matched with the internal threads on the first cover 2 to connect and fix the first cover 2. The processing assembly 3 is arranged on the first cover 2 and comprises a guide tube 31, at least one row of teeth 32 and an agitation element 33.
The guide tube 31 passes through the first cap 2 through a center hole (not shown) of the first cap 2 to be rotatable with respect to the first cap 2. In the present embodiment, three rows of teeth 32 are arranged around the guide tube 31, at different levels, fixed to the first cover 2. The stirring member 33 is fixed to the upper outer surface of the guide tube 31, extends downward, and is rotatable with respect to the three rows of teeth 32. Wherein the stirring element 33 comprises a cantilevered cutting blade 331 and a structure 332 cooperating with the three rows of teeth 32. As an embodiment of the present invention, the cantilever cutting blade 331 may be provided in plurality. The structural member 332 extends from the guide tube 31 and covers the three rows of teeth 32 with a relatively short distance between the corresponding surfaces of the structural member 332 and the three rows of teeth 32. The distance is preferably about 100um for preparing single cell suspensions and about 20um for preparing homogenates (biological samples with disrupted cell structures, release of proteins inside the cells and accounting) depending on the particle size of the biological sample to be prepared. The lower inner surface of the guide tube 31 includes a coupling portion (not shown) for coupling with an external drive shaft. When the experimental container is used for treating tissues, manually cut tissues are placed into the experimental container, the external driving shaft is connected with the joint part of the guiding pipe 31, the guiding pipe 31 and the stirring element 33 are driven to rotate when the external driving shaft rotates, the container body 1, the first cover 2 and the three rows of teeth 32 fixed on the first cover 2 are not moved, the tissues in the experimental container are subjected to cutting by the cantilever type cutting knife 331 and then are subjected to grinding by the structural part 332, and the circulation treatment is carried out. The rotating speed and the processing time are comprehensively set according to the requirements of the tissue adhesiveness and the granularity of the biological sample required to be prepared.
According to the embodiment of the invention, the tissue put into the experimental container is cut by the rotatable cantilever type cutting knife, the tissue put into the experimental container is ground by the plurality of rows of teeth fixed on the first cover and the rotatable structural member covered on the first cover, and when the cantilever type cutting knife and the structural member rotate, the tissue in the experimental container is repeatedly cut and ground, so that cells in the prepared biological sample are more fully dispersed.
Example two
In the embodiment of the present invention, the basic structure of the experimental container is the same as that of the first embodiment, and the same reference numerals are used for the same components as those of the first embodiment, including all the features described in the first embodiment, and thus, the description thereof is omitted.
Fig. 2 is a side view of the first cover 2 and the processing assembly 3 according to the second embodiment of the present invention. Fig. 3 is a plan view of the first cover 2 and the processing assembly 3 according to the second embodiment of the present invention. As shown in fig. 2 and 3, in an embodiment of the present invention, the structural member 332 further comprises a helical baffle 3321 forming a predetermined angle with the three rows of teeth 32. As the structure 332 rotates, the tissue to be treated is pushed by the helical baffle 3321 towards the cantilevered cutting blade 331, undergoing cutting by the cantilevered cutting blade 331. Baffle 3321 is configured in a helical configuration to reduce the mechanical resistance of the tissue to be treated to which structure 332 is subjected during rotation. Preferably, at least one pressure relief opening a is formed in the edge of the spiral-shaped baffle 3321, and the pressure relief opening a is a narrow slit allowing the biological sample processed into small particles to pass through, so as to further reduce the mechanical resistance of the tissue to be processed to the structural member 332 during rotation.
One end of the cantilever type cutting knife 331 is fixed to the guide tube 31 and then extends outward, the cutting knife having cutting edges at both ends in the vertical direction, and the end of the cutting knife extends above the three rows of teeth 32, preferably, the step between the middle row of teeth and the lowest row of teeth. Fig. 4 is a schematic view of the region B in fig. 3. As shown in FIG. 4, the cross section of the cutting blade in the vertical direction of the cantilever type cutting blade 331 is fan-shaped, and both ends are cutting edges, which can cut the tissue to be treated contacted in the rotation. For the same cross section, the side of the cutting blade facing the middle row of teeth in the vertical direction of the cantilever cutting blade 331 and the periphery of the middle row of teeth (indicated by a circular arc) form a triangle-like shape, i.e. d1 is larger than d2 in fig. 4, but the narrowest part still keeps a certain narrow distance, so that the cut tissue to be treated can pass through and can be extruded and ground to a certain extent when passing through.
The cantilever type cutting knife 331 realizes rough cutting of the tissue to be treated, the structural member 332 realizes fine grinding of the tissue to be treated, and the two processes of rough cutting and fine grinding are completed by two different assemblies, so that cells in the prepared biological sample are dispersed more fully.
As an embodiment of the present invention, the structure 332 and the cantilever cutting blade 331 are symmetrically distributed with respect to the guide tube 31, and may be disposed at an included angle of 90 degrees, 45 degrees, or the like, as viewed from a top view.
To prevent leakage, the guide tube 31 and the three rows of teeth 32 are connected by a seal 4. Further, as shown in fig. 1, the experimental container further includes an absorption ring 5 for preventing leakage and a second cover 6. The absorption ring 5 is sleeved on a lower portion of the guide pipe 31 and is received in the second cover 6. The second cap 6 is fixed to the first cap 2 and also serves as a container for receiving leaked liquid.
According to the embodiment of the invention, the tissue put into the experimental container is cut by the rotatable cantilever type cutting knife, the tissue put into the experimental container is ground by the plurality of rows of teeth fixed on the first cover and the rotatable structural member covered on the first cover, and when the cantilever type cutting knife and the structural member rotate, the tissue in the experimental container is repeatedly cut and ground, so that cells in the prepared biological sample are more fully dispersed.
EXAMPLE III
Fig. 5 is a schematic structural diagram of a biological sample preparation device according to a third embodiment of the present invention. With reference to fig. 1 and 5, a biological sample preparation device includes a housing, a motor, and an experimental vessel as described in example one or example two. The motor sets up in the shell, and the breach of shell is put into to the experiment container, and vessel 1 and first lid 2 are fixed, and the drive shaft and the guide tube 31 of motor are connected, and the drive experiment container is rotatory in order to prepare biological sample.
According to the embodiment of the invention, the tissue put into the experimental container is cut by the rotatable cantilever type cutting knife, the tissue put into the experimental container is ground by the plurality of rows of teeth fixed on the first cover and the rotatable structural member covered on the first cover, and when the cantilever type cutting knife and the structural member rotate, the tissue in the experimental container is repeatedly cut and ground, so that cells in the prepared biological sample are more fully dispersed.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (10)
1. An assay container, comprising:
a container body (1) having an open end (11) and a closed end (12);
a first lid (2) connected to an open end (11) of the container body (1); and
a processing assembly (3) arranged on the first cover (2);
wherein the treatment assembly (3) comprises a guide tube (31) rotatable relative to the first cover (2) through the first cover (2), at least one row of teeth (32) fixed on the first cover (2) arranged around the guide tube (31), and an agitation element (33) fixed on the guide tube (31);
wherein the stirring element (33) comprises at least one cantilever cutting knife (331) and a structure (332) cooperating with the at least one row of teeth (32), the structure (332) extending from the guide tube (31) and covering the at least one row of teeth (32).
2. The assay container according to claim 1, wherein, for the same cross-section, the side of the at least one cantilever cutting blade (331) facing the at least one row of teeth (32) forms a triangle-like shape with the periphery of the at least one row of teeth (32).
3. The assay container according to claim 1, wherein the at least one cantilever cutting blade (331) is fan-shaped in cross-section.
4. The cuvette according to claim 1, wherein the structure (332) further comprises a helical baffle (3321) forming a predetermined angle with the at least one row of teeth (32).
5. The vessel according to claim 4, wherein at least one pressure relief opening (A) is provided in the edge of the spiral-shaped baffle (3321).
6. The cuvette according to any of claims 1 to 5, wherein the structure (332) and the at least one cantilever cutting knife (331) are symmetrically distributed about the guide tube (31).
7. Laboratory vessel according to any of claims 1 to 5, characterized in that the guide tube (31) and the at least one row of teeth (32) are connected by a seal (4).
8. The assay container according to any of claims 1-5, further comprising an absorption ring (5) and a second lid (6) for leakage prevention.
9. Laboratory vessel according to anyone of claims 1 to 5, characterized in that the guide tube (31) further comprises a coupling adapted for connection of an external drive shaft.
10. A biological sample preparation device, comprising a housing, a motor and a test vessel according to any of claims 1 to 9, wherein the motor is arranged in the housing, and wherein a drive shaft of the motor is connected to the guide tube (31) for driving the test vessel in rotation for preparing a biological sample.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110473378.3A CN113176123A (en) | 2021-04-29 | 2021-04-29 | Experimental container and biological sample preparation device |
| PCT/CN2021/114998 WO2022227354A1 (en) | 2021-04-29 | 2021-08-27 | Experimental container and biological sample preparation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110473378.3A CN113176123A (en) | 2021-04-29 | 2021-04-29 | Experimental container and biological sample preparation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113176123A true CN113176123A (en) | 2021-07-27 |
Family
ID=76925167
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110473378.3A Pending CN113176123A (en) | 2021-04-29 | 2021-04-29 | Experimental container and biological sample preparation device |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN113176123A (en) |
| WO (1) | WO2022227354A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113930321A (en) * | 2021-11-12 | 2022-01-14 | 青岛华大智造科技有限责任公司 | Dissociation assembly, tissue dissociation tube, dissociation device and tissue dissociation method |
| WO2022227354A1 (en) * | 2021-04-29 | 2022-11-03 | 深圳市瑞沃德生命科技有限公司 | Experimental container and biological sample preparation device |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100203521A1 (en) * | 2007-04-02 | 2010-08-12 | Boston Medical Center Corporation | Method for bacterial lysis |
| WO2009111338A1 (en) * | 2008-02-29 | 2009-09-11 | Biomet Manufacturing Corp. | A system and process for separating a material |
| ES2574666T3 (en) * | 2011-06-27 | 2016-06-21 | Miltenyi Biotec Gmbh | Tissue fragment device |
| DE102013007063A1 (en) * | 2013-04-19 | 2014-10-23 | Kocher-Plastik Maschinenbau Gmbh | container |
| CN210544904U (en) * | 2019-08-07 | 2020-05-19 | 北京海思特医学检验实验室有限公司 | Separated single cell suspension preparation device |
| CN211339485U (en) * | 2019-08-22 | 2020-08-25 | 广州市尤德生物科技有限公司 | Tissue separator for preparing single cell suspension |
| CN113176123A (en) * | 2021-04-29 | 2021-07-27 | 深圳市瑞沃德生命科技有限公司 | Experimental container and biological sample preparation device |
| CN215162615U (en) * | 2021-06-08 | 2021-12-14 | 北京极客基因科技有限公司 | Single cell suspension preparation facilities |
-
2021
- 2021-04-29 CN CN202110473378.3A patent/CN113176123A/en active Pending
- 2021-08-27 WO PCT/CN2021/114998 patent/WO2022227354A1/en unknown
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022227354A1 (en) * | 2021-04-29 | 2022-11-03 | 深圳市瑞沃德生命科技有限公司 | Experimental container and biological sample preparation device |
| CN113930321A (en) * | 2021-11-12 | 2022-01-14 | 青岛华大智造科技有限责任公司 | Dissociation assembly, tissue dissociation tube, dissociation device and tissue dissociation method |
| CN113930321B (en) * | 2021-11-12 | 2023-12-08 | 青岛华大智造科技有限责任公司 | Dissociation assembly, tissue dissociation tube, dissociation device and tissue dissociation method |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2022227354A1 (en) | 2022-11-03 |
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