CN211339485U

CN211339485U - Tissue separator for preparing single cell suspension

Info

Publication number: CN211339485U
Application number: CN201921377084.5U
Authority: CN
Inventors: 吴晓春; 李建华; 杨光世; 黄岭
Original assignee: Unitech Bioscience Co ltd
Current assignee: Unitech Bioscience Co ltd
Priority date: 2019-08-22
Filing date: 2019-08-22
Publication date: 2020-08-25
Anticipated expiration: 2029-08-22

Abstract

The utility model discloses a tissue separator of preparation unicellular suspension belongs to the tissue separation field, including cup, cutter arbor and the blade of connection in the cutter arbor bottom, the blade is located the cup, and the cutter arbor upper portion stretches out from cup open-top, the location is provided with the cell screen cloth in the cup, and this cell screen cloth is with the inside cutting chamber that divide into the top of cup and the suspension chamber that is located the below, and the sieve mesh intercommunication cutting chamber and the suspension chamber of cell screen cloth, cell screen cloth are provided with the cutting edge corresponding to the border position of every sieve mesh, the blade is located cutting intracavity and the blade under the terminal surface butt cutting edge up end. The utility model discloses at first utilize the cutting action that lasts between the cutting edge on the cell screen cloth and the rotatory blade thoroughly to separate the sample, later the suspension falls into the suspension chamber downwards through the filtration of sieve mesh and supplies direct utilization, and it has accomplished separation, filterable function at a separator.

Description

Tissue separator for preparing single cell suspension

Technical Field

The utility model relates to an equipment facility field of separation living cell especially relates to a tissue separator of preparation unicellular suspension.

Background

CN203639459U and CN204079962U disclose tissue processors for separating living cells, respectively, wherein a blade (head) is disposed in a cup body, and the rotation of the blade (head) homogenizes a tissue sample. Although the structure can uniformly beat the sample to a certain degree, the tissue separation efficiency is not high, the final living cell suspension can be obtained only by filtering the slurry at the later stage, the efficiency is undoubtedly low, and the pollution of an intermediate link can be formed.

SUMMERY OF THE UTILITY MODEL

In order to solve the problem, the utility model provides a tissue separator for preparing single cell suspension, its separation effect is good, efficient.

The utility model adopts the technical proposal that:

the utility model provides a tissue separator of preparation unicellular suspension, includes cup, cutter arbor and connects the blade in the cutter arbor bottom, the blade is located the cup, and the cutter arbor upper portion stretches out from the cup open-top, the location is provided with the cell screen cloth in the cup, and this cell screen cloth is with the inside cutting chamber that divide into the top of cup and the suspension chamber that is located the below, and the sieve mesh intercommunication cutting chamber and the suspension chamber of cell screen cloth, cell screen cloth are provided with the cutting edge corresponding to the border position of every sieve mesh, the blade is located the cutting intracavity and the blade under the terminal surface butt cutting edge up end.

As a further improvement of the utility model, the cutting edge is formed by extending upwards the position of the cell screen corresponding to the edge of the screen hole.

As a further improvement, the sieve mesh is a polygon, each edge of the polygon is provided with a cutting edge, and the top end of each cutting edge is enclosed into the inlet of the sieve mesh.

As a further improvement of the utility model, the sieve mesh is a regular hexagon.

As a further improvement of the utility model, the top and the bottom of blade pass through inclined plane or cambered surface transition, and the bottom of blade is equipped with the cutting edge with the contact of cell screen cloth.

As a further improvement of the present invention, the blade only has a cutting edge contacting the cell screen.

As a further improvement of the utility model, the suspension intracavity is equipped with the stirring leaf, the cutter arbor bottom is connected to the stirring leaf.

As a further improvement of the utility model, the cup includes the base, the cover body of nestification in the base and the support of nestification in the cover body, and base, the cover body and support are transition fit, and the cutter arbor penetrates the cover body from the support middle part, the cell screen cloth is by the centre gripping between support bottom face and cover body bottom.

As a further improvement of the utility model, the upper end face of the cup body is provided with a hole, and the hole is communicated with the suspension cavity.

As a further improvement of the utility model, the upper end of the cup body is detachably covered with a cup cover, and the cup cover covers the hole.

The utility model has the advantages that: the utility model discloses at first utilize the cutting action that lasts between the cutting edge on the cell screen cloth and the rotatory blade thoroughly to separate the sample, later the suspension falls into the suspension chamber downwards through the filtration of sieve mesh and supplies direct utilization, and it has accomplished separation, filterable function at a separator.

Drawings

The present invention will be further described with reference to the accompanying drawings and embodiments.

FIG. 1 is a schematic view of a tissue separator;

FIG. 2 is a schematic view of a cell screen;

FIG. 3 is a top view of a cell screen;

FIG. 4 is an enlarged schematic view of portion A of FIG. 3;

FIG. 5 is a schematic view of the mounting of the blade to the cell screen.

Detailed Description

The tissue separator for preparing single cell suspension as shown in fig. 1 comprises a cup body 1, a knife bar 2 and a blade 3 connected to the bottom of the knife bar 2. The blade 3 sets up in cup 1, and cutter arbor 2 is along vertical arrangement, and upper portion stretches out from 1 top opening of cup. The top of the knife bar 2 is a connecting part 21 for connecting an external driving mechanism, such as a motor, and the driving mechanism drives the knife bar 2 to rotate so as to drive the blade 3 to rotate in the cup body 1.

A cell screen 4 is transversely positioned in the cup body 1, and a plurality of sieve holes 8 shown in figures 2 to 4 are uniformly distributed on the cell screen 4. The cell screen 4 divides the inside of the cup body 1 into a cutting cavity 5 at the upper part and a suspension cavity 6 at the lower part, each screen hole 8 is communicated with the cutting cavity 5 and the suspension cavity 6, and the blade 3 is positioned in the cutting cavity 5. The upper surface of the cell screen 4 is provided with a cutting edge 7 corresponding to the edge of each screen hole 8, the blade 3 is positioned in the cutting cavity 5, and the lower end surface of the blade 3 is abutted against the upper end surface of the cutting edge 7. The rotation of the blade 3 may produce a friction cutting action with the cutting edge 7.

When the cell cutting device is used, a cell sample (solid state) is added into the cutting cavity 5, liquid (which can be injected from the cutting cavity or injected through other ways) is injected into the suspension cavity 6, the driving mechanism is started, the blade 3 starts to rotate, and the blade 3 drives the sample to rotate in the process because the sample is supported by the cell screen 4, so that the sample is pressed below the blade 3, the sample, the blade 3 and the blade edge 7 continuously generate the friction cutting effect, and the sample is divided into fine particles; the fine particles and the liquid are fully mixed to form suspension, because the blade 7 is just positioned at the edge of the sieve pore 8, the mixed liquid mixed with the fine particles immediately passes through the sieve pore 8 and is mixed with the liquid in the suspension cavity 6 under the action of gravity, and the larger particles cannot pass through the sieve pore 8, namely the sieve pore 8 plays a role in filtering at the same time. In this way, the blade 3 continues to cut the sample, the blade 7, i.e. repeatedly cut the sample, until the sample meets the appropriate particle size and falls through the mesh 8 into the suspension chamber 6, completing the tissue separation. Finally, the single cell suspension can be directly obtained from the suspension chamber 6 by stopping the rotation of the blade 3.

Further preferably, referring to fig. 2 to 4, the knife edge 7 is integrally formed with the cell screen 4, and is made of a metal material. Each blade 7 is formed by extending and protruding the cell screen 4 at a position corresponding to the edge of the screen hole 8. The forming mode of the cutting edge 7 does not need extra assembling steps, is more convenient to install, does not have a gap between the cutting edge 7 and the cell screen 4, and does not have the problem of loose connection between the cutting edge 7 and the cell screen.

It is further preferred that the sieve openings 8 are arranged in the shape of a polygon, each side of the polygon being provided with a knife edge 7. The polygonal screen openings allow an angle to be formed between adjacent cutting edges 7, a plurality of which has a more advantageous effect on the cutting action. The top end of each blade 7 encloses the inlet of a polygonal sieve opening 8 so that after cutting, suitably sized particles can fall directly down into the sieve opening 8 to the suspension chamber 6.

The preferred screen holes 8 are regular hexagons, and the corresponding screen holes 8 also have six blades 7 enclosing a regular hexagon.

Further preferably, reference is made to fig. 5, in which the screening holes are not illustrated, but do not affect the understanding thereof by the person skilled in the art. The blade 3 is approximately fan-shaped, the top and the bottom of the blade are transited through a cambered surface, and the bottom of the blade is contacted with the cell screen 4. Below this curved surface is then formed a collecting zone 9, which collecting zone 9 can also be seen as an opening towards the direction of rotation of the blade 3, which opening tapers in the opposite direction of the direction of rotation of the blade 3. Therefore, during the rotation of the blade 3, the mixed liquor can reach the bottom position of the blade 3 oppositely along the guiding action of the cambered surface of the collecting area 9 and cut at the corresponding position. That is, the blade having such a shape has a function of collecting the mixed liquid and guiding the mixed liquid.

In another embodiment, not shown, the above-mentioned arc surface may also be replaced by a bevel, which has a similar effect.

The bottom end of the blade 3 is provided with a cutting edge 11, and the cutting edge 11 is continuously contacted and rubbed with the cell screen 4 while the blade rotates, and will cut the sample.

Further, the cutting edge 11 is bent downward with respect to the entire blade 3, so that only the cutting edge 11 of the entire blade 3 is in contact with the cell screen 4, thereby preventing the blade from being in excessive grinding contact with the sample solution.

The cutting surface 10 and the cutting edge 11 are connected by a transition section 15 that is arched upward and spaced apart from the cell screen 4. The position arched to the cutting edge 11 forms a cambered surface from high to low similar to the collecting area 9, so that a sample can be collected from the side end for cutting, and the tissue separation efficiency is further improved.

Further preferably, referring to fig. 1, a stirring blade 12 is arranged in the suspension chamber 6, the stirring blade 12 is connected to the bottom end of the cutter bar 2, and rotates in a linkage manner in the rotation process of the cutter blade 3 to stir the suspension, so that the suspension is kept uniform.

Further preferably, the cup body 1 is a detachable structure, and includes a base 101, a sleeve 102 nested in the base 101, and a bracket 103 nested in the sleeve 102. The base 101, the sleeve body 102 and the bracket 103 are in transition fit, the connecting surface between the base 101 and the sleeve body 102 includes a guiding inclined surface not shown in the figure, the connecting surface between the bracket 103 and the sleeve body 102 also includes another guiding inclined surface not shown in the figure, and the two guiding inclined surfaces are convenient for the tight connection of each component. The base 101, the sleeve 102 and the bracket 103 together enclose the inner cavity of the cup body 1.

Wherein, the knife bar 2 is fixed in the middle of the bracket 103 and penetrates into the sheath 102 from the middle of the bracket 103, and the cell screen 4 is clamped between the bottom end surface of the bracket 103 and the bottom of the sheath 102.

It is further preferred that the upper end surface of the cup body 1 is provided with a hole 13, the hole 13 is communicated with the suspension cavity 6, and the hole 13 is preferably arranged on the upper end surface of the sleeve body 102. The hole 13 can be used as a liquid adding hole of the cup body 1, and liquid mixed with the sample is directly added; the holes 13 can also be used as circulation holes of the cup body 1, the mixed liquid in the suspension cavity 6 is lifted by external lifting equipment and then returns to the cutting cavity 5 for multiple cutting, and the particle size of particles is further reduced; the hole 13 can also be used as a liquid taking port to directly take the suspension from the suspension cavity 6.

The upper end of the cup body 1 is generally opened, a sample can be placed in the cup body, liquid can also be directly injected into the cup body, a cup cover 14 is detachably covered at the upper end of the cup body 1, and the cup cover 14 covers the hole 13. Lid 14 may be opened for tissue separation.

The above description is only a preferred embodiment of the present invention, and does not limit the scope of the present invention.

Claims

1. The utility model provides a tissue separator of preparation single cell suspension, includes cup (1), cutter arbor (2) and connects blade (3) in cutter arbor (2) bottom, blade (3) are located cup (1), and cutter arbor (2) upper portion stretches out its characterized in that from cup (1) open-top: the cell sieve is characterized in that a cell screen (4) is positioned in the cup body (1), the cell sieve (4) divides the inside of the cup body (1) into a cutting cavity (5) above and a suspension cavity (6) below, sieve holes (8) of the cell sieve (4) are communicated with the cutting cavity (5) and the suspension cavity (6), a cutting edge (7) is arranged at the edge position of the cell sieve (4) corresponding to each sieve hole (8), and the blade (3) is positioned in the cutting cavity (5) and abuts against the upper end face of the cutting edge (7) at the lower end face of the blade (3).

2. The tissue separator for preparing a single cell suspension of claim 1, wherein: the blade (7) is formed by extending the cell screen (4) upwards at the position corresponding to the edge of the sieve hole (8).

3. The tissue separator for preparing a single cell suspension of claim 2, wherein: the sieve mesh (8) is polygonal, each edge of the polygon is provided with a cutting edge (7), and the top end of each cutting edge (7) is enclosed to form an inlet of the sieve mesh (8).

4. The tissue separator for preparing a single cell suspension of claim 3, wherein: the sieve holes (8) are in the shape of a regular hexagon.

5. The tissue separator for preparing single cell suspension according to claim 1 or 2 or 3 or 4, characterized in that: the top and the bottom of the blade (3) are transited through an inclined plane or an arc surface, and the bottom end of the blade (3) is provided with a cutting edge (11) contacted with the cell screen (4).

6. The tissue separator for preparing a single cell suspension of claim 5, wherein: only the cutting edge (11) of the blade (3) contacts the cell screen (4).

7. The tissue separator for preparing single cell suspension according to claim 1 or 2 or 3 or 4, characterized in that: and a stirring blade (12) is arranged in the suspension cavity (6), and the stirring blade (12) is connected with the bottom end of the cutter bar (2).

8. The tissue separator for preparing single cell suspension according to claim 1 or 2 or 3 or 4, characterized in that: the cell screen comprises a cup body (1) and a cutter bar (2), wherein the cup body comprises a base (101), a sleeve body (102) embedded in the base (101) and a support (103) embedded in the sleeve body (102), the base (101), the sleeve body (102) and the support (103) are in transition fit, the cutter bar (2) penetrates into the sleeve body (102) from the middle of the support (103), and the cell screen (4) is clamped between the bottom end face of the support (103) and the bottom of the sleeve body (102).

9. The tissue separator for preparing a single cell suspension of claim 8, wherein: the cup is characterized in that a hole (13) is formed in the upper end face of the cup body (1), and the hole (13) is communicated with the suspension cavity (6).

10. The tissue separator for preparing a single cell suspension of claim 9, wherein: a cup cover (14) is detachably covered at the upper end of the cup body (1), and the cup cover (14) covers the hole (13).