CN114164109B

CN114164109B - Tissue dissociation tube

Info

Publication number: CN114164109B
Application number: CN202111453284.6A
Authority: CN
Inventors: 徐传来; 邱匀彦; 鲍宇; 储冬东; 高缘缘; 钱梦雅
Original assignee: Nanjing Tongyuan Medical Laboratory Co ltd; Suzhou New Geyuan Biotechnology Co ltd
Current assignee: Nanjing Tongyuan Medical Laboratory Co ltd; Suzhou New Geyuan Biotechnology Co ltd
Priority date: 2021-12-01
Filing date: 2021-12-01
Publication date: 2024-02-23
Anticipated expiration: 2041-12-01
Also published as: CN114164109A

Abstract

The invention belongs to the technical field of biological experimental instruments, and discloses a tissue dissociation tube, which comprises a tube body, wherein a first grinding body, a second grinding body, a support frame and a filter screen which are provided with grinding grooves are sequentially arranged in the tube body, the second grinding body comprises a mounting plate and a grinding head, the mounting plate is arranged on one side of a notch of the grinding groove, the mounting plate and the grinding groove form a containing space, the grinding head is arranged in the containing space, a grinding space for containing tissues to be ground is arranged between the grinding head and the grinding groove, the first grinding body is rotationally connected with the second grinding body, an external power output device can drive one of the first grinding body and the second grinding body to rotate so as to grind the tissues to be ground, and a plurality of first through grooves are formed in the periphery of the mounting plate; a plurality of second through grooves are formed in the circumferential direction of the support frame, the support frame can be abutted to or separated from the mounting plate, and the first through grooves and the second through grooves are staggered. The invention simplifies the structure and forms an integrated structure, is convenient to operate, and can prevent tissue leakage in the grinding process.

Description

Tissue dissociation tube

Technical Field

The invention relates to the technical field of biological experiment instruments, in particular to a tissue dissociation tube.

Background

Tissue dissociation, as the name implies, is a technique of breaking down and dispersing clumped animal and plant tissue, such as heart tissue of mice for testing, artificially cultured kidney tissue of humans, etc., into single cell suspensions. In single cell sequencing and related techniques in biological research, single cells need to be processed and analyzed, but in practical experiments, only a block of tissue can be obtained from sample tissue, not ready single cells. At this point, tissue dissociation is required to obtain a single cell suspension, allowing subsequent investigation of capture, lysis or other procedures. The existing tissue dissociation technology is mature, but the grinding structure and the filtering structure are separately arranged, the structure is complex, the operation is inconvenient, tissue leakage in grinding possibly exists in the grinding process, insufficient grinding or the obtained cell suspension is poor in effect, and the requirement on test personnel is high.

Disclosure of Invention

The invention aims to provide a tissue dissociation tube which has a simplified structure and an integrated structure, is convenient to operate, can prevent tissue leakage in the grinding process, ensures that the tissue is sufficiently ground, and improves the cell suspension effect.

To achieve the purpose, the invention adopts the following technical scheme:

a tissue dissociation tube comprising a tube body, wherein the tube body is internally provided with:

a first grinding body having a grinding groove;

the second grinding body comprises a mounting plate and a grinding head, wherein the mounting plate is arranged on one side of a notch of the grinding groove, the mounting plate and the grinding groove form a containing space, the grinding head is arranged in the containing space, a grinding space for containing tissues to be ground is formed between the grinding head and the grinding groove, the first grinding body is rotationally connected with the second grinding body, an external power output device can drive one of the first grinding body and the second grinding body to rotate so as to grind the tissues to be ground, and a plurality of first through grooves are formed in the periphery of the mounting plate;

the support frame is provided with a plurality of second through grooves in the circumferential direction, can be abutted against or separated from one side of the mounting plate, which is away from the grinding head, and is staggered with the first through grooves;

the filter screen is arranged on one side of the support frame, which is away from the second grinding body.

Optionally, the periphery of the grinding head is provided with a plurality of first cutting blades which are arranged at intervals.

Optionally, the first cutting edge has a tooth-shaped structure.

Optionally, the first grinding body comprises a shell and a first connecting shaft arranged in the shell, the first connecting shaft is rotationally connected with the grinding head, and the grinding groove is formed in the shell.

Optionally, the pipe body is the transparent body, the casing includes interconnect's shell bottom plate and semi-ring plate, the shell bottom plate install in on the first connecting axle.

Optionally, the semi-ring plate has offered a plurality of cell bodies in succession along circumference, and the degree of depth of a plurality of cell bodies increases in proper order, the second grinding body can be relative first grinding body is followed the one side that the cell body degree of depth is big is to the one side that the cell body degree of depth is little rotates.

Optionally, the tissue dissociation tube further comprises a cover body, the cover body is covered at one end of the tube body, and the cover body is detachably connected with the tube body.

Optionally, the first grinding body further comprises a second connecting shaft, the second connecting shaft is arranged outside the shell, the second connecting shaft penetrates through the cover body, and the external power output device is connected with the second connecting shaft.

Optionally, the filter screen is mounted on the tube or the support frame.

Optionally, an extension rod is arranged at one side of the mounting plate, which is away from the grinding head, and a limiting block is arranged at the end part of the extension rod;

the periphery of the support frame is provided with a first groove and a second groove which are communicated with each other, and the groove depth of the second groove along the radial direction of the support frame is larger than that of the first groove along the support frame;

the extension rod can be arranged between the first groove and the penetrating hole formed by the pipe body in a penetrating mode, the limiting block is connected in the second groove in a sliding mode, and the limiting block can be blocked by the groove wall of the first groove.

The invention has the beneficial effects that:

according to the tissue dissociation tube provided by the invention, the first grinding body and the second grinding body are arranged in the tube body, and the support frame can support the second grinding body and further support the first grinding body, so that the structure is stable; by arranging the supporting frame and the mounting plate structure, the tissue can be fully ground, dissociated tissue is output, leakage of the tissue which is not fully ground in the grinding process is avoided, the grinding is more sufficient, and the effect of the obtained cell suspension is improved; the filter screen is arranged for filtering again, so that the tissue which is not ground is prevented from being output, and the effect of the output cell suspension of the dissociated tissue is further improved. The first grinding body, the second grinding body, the supporting frame, the filter screen and the pipe body are integrated to form a whole, so that the structure is simple, and the portable grinding machine is convenient to carry and use.

Drawings

FIG. 1 is an exploded view of a tissue dissociation tube provided in accordance with an embodiment of the present invention;

FIG. 2 is a longitudinal cross-sectional view of a tissue dissociation tube provided by an embodiment of the present invention;

fig. 3 is a schematic structural view of a second polishing body in an abutting state with a supporting frame according to an embodiment of the present invention;

fig. 4 is a schematic structural diagram of a second polishing body and a supporting frame in a separated state according to an embodiment of the present invention.

In the figure:

1. a tube body; 11. a filter screen; 12. a supporting protrusion;

2. a first abrasive body; 21. a housing; 211. a shell bottom plate; 212. a semi-annular plate; 2121. a tank body; 22. a first connecting shaft; 23. a second connecting shaft;

3. a second abrasive body; 31. a mounting plate; 31A, a receiving space; 311. a first through groove; 32. a grinding head; 321. a first cutting edge; 33. an extension rod; 34. a limiting block;

4. a support frame; 41. a second through slot; 42. a first groove; 43. a second groove;

5. a cover body; 51. an end portion; 52. an extending portion.

Detailed Description

In order to make the technical problems solved by the present invention, the technical solutions adopted and the technical effects achieved more clear, the technical solutions of the embodiments of the present invention will be described in further detail below with reference to the accompanying drawings, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

In the description of the present invention, unless explicitly stated and limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.

In the present invention, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, as well as the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.

The embodiment provides a tissue dissociation tube, as shown in fig. 1-4, which comprises a tube body 1, wherein a first grinding body 2, a second grinding body 3, a supporting frame 4 and a filter screen 11 are arranged in the tube body 1; specifically, the first polishing body 2 has a polishing groove; the second grinding body 3 comprises a mounting plate 31 and a grinding head 32, the mounting plate 31 is arranged on one side of a notch of the grinding groove, the mounting plate 31 and the grinding groove form a containing space 31A, the grinding head 32 is arranged in the containing space 31A, a grinding space for containing tissues to be ground is formed between the grinding head 32 and the grinding groove, the first grinding body 2 is rotationally connected with the second grinding body 3, an external power output device can drive one of the first grinding body 2 and the second grinding body 3 to rotate so as to grind the tissues to be ground, and a plurality of first through grooves 311 are formed in the periphery of the mounting plate 31; a plurality of second through grooves 41 are formed in the circumferential direction of the support frame 4, the support frame 4 can be abutted against or separated from one side of the mounting plate 31, which is away from the grinding head 32, and the plurality of first through grooves 311 and the plurality of second through grooves 41 are staggered; the filter screen 11 is arranged on the side of the support frame 4 facing away from the second grinding body 3.

Before use, as shown in fig. 3, the support frame 4 is abutted against one side of the mounting plate 31, which is away from the grinding head 32, so that the first through groove 311 and the second through groove 41 are not communicated, and leakage of tissue which is not completely dissociated in the grinding space is avoided; when the device is used, the external power output device drives the first grinding body 2 or the second grinding body 3 to rotate, so that the first grinding body 2 and the second grinding body 3 relatively rotate, and tissues to be ground in the grinding space are ground in the rotating process; after polishing, as shown in fig. 4, the support frame 4 is separated from the mounting plate 31, the first through groove 311 and the second through groove 41 are communicated, and the dissociated tissue in the polishing space is sequentially fed into the filter screen 11 on the other side of the support frame 4 through the first through groove 311 and the second through groove 41, passes through the filter screen 11, and is then output.

The first grinding body 2 and the second grinding body 3 are arranged in the pipe body 1, and the supporting frame 4 can support the second grinding body 3 and further support the first grinding body 2, so that the structure is stable; by arranging the structures of the support frame 4 and the mounting plate 31, the sufficient grinding can be ensured to ensure that tissues are sufficiently ground, dissociated tissues are output, the leakage of tissues which are not sufficiently ground in the grinding process is avoided, the grinding is more sufficient, and the obtained cell suspension effect is improved; by arranging the filter screen 11 for filtering again, the tissues which are not ground are prevented from being output, the effect of the output cell suspension of the dissociated tissues is further improved, and the requirements on test staff are further reduced. The first grinding body 2, the second grinding body 3, the supporting frame 4, the filter screen 11 and the pipe body 1 are integrated to form a whole, and the portable grinding machine is simple in structure and convenient to carry and use.

Optionally, at least one of the first grinding body 2 and the second grinding body 3 has a cutting blade, improving the grinding effect. Optionally, as shown in fig. 1, the outer periphery of the grinding head 32 has a plurality of first cutting edges 321 arranged at intervals, so that the number of the first cutting edges 321 is increased, and the cutting and grinding efficiency is further improved; further, the first cutting blades 321 have the same structure and are uniformly arranged at intervals, so that the grinding and dissociation effects are improved, and the grinding and dissociation device is convenient to manufacture and uniform in stress. In this embodiment, the first cutting edge 321 has a tooth-shaped structure, has a good grinding effect, and is convenient to manufacture.

Alternatively, as shown in fig. 1 to 4, the first grinding body includes a housing 21 and a first connecting shaft 22 disposed in the housing 21, the first connecting shaft 22 is rotatably connected with the grinding bit 32, and a grinding groove is formed in the housing 21, so that the first grinding body is compact, and the first grinding body and the second grinding body are more compactly mounted.

Alternatively, as shown in fig. 1 to 4, the pipe body 1 is a transparent body, the casing 21 includes a casing bottom plate 211 and a half ring plate 212 that are connected to each other, the casing bottom plate 211 is mounted on the first connecting shaft 22, the half ring plate 212 is mounted on the first connecting shaft 22 through the casing bottom plate 211, the half casing 21 is formed by arranging the half ring plate 212, and the pipe body 1 is a transparent body, so that an operator can observe the internal grinding condition in the grinding process, so as to determine whether the dissociation requirement is reached, and then perform the next work. Specifically, the tube body 1 may be made of transparent plastic or glass.

Optionally, as shown in fig. 1, the half ring plate 212 is continuously provided with a plurality of grooves 2121 along the circumferential direction, the depths of the plurality of grooves 2121 are sequentially increased, the second grinding body 3 can rotate relative to the first grinding body 2 from the side with the larger depth of the grooves 2121 to the side with the smaller depth of the grooves 2121, during the grinding process, tissues are gradually dissociated along with grinding, the structural components are reduced, the grinding resistance is gradually reduced, during the relative rotation process of the first grinding body 2 and the second grinding body 3, along with the sequential reduction of the depths of the grooves 2121, the grinding space is gradually reduced, and the tissues are gradually dissociated along with the dissociation, so that the structure of the grinding body is smaller and smaller; the groove 2121 with larger depth forms larger grinding space, can accommodate larger tissue structure, reduces grinding resistance and is convenient for grinding; the groove 2121 with smaller depth forms smaller grinding space, increases grinding force and improves grinding fineness. Specifically, the maximum depth of the groove 2121 is set according to the structure size of the tissue to be ground placed in the groove 2121 at the beginning, and the minimum depth of the groove 2121 is set according to the required grinding effect; the number of the arrangement of the respective grooves 2121, the difference in depth, and the like may be set according to actual conditions, and are not limited. In addition, the side wall between two adjacent groove bodies 2121 can form a cutting edge, so that the grinding dissociation efficiency is further improved.

Optionally, as shown in fig. 1 and fig. 2, the tissue dissociation tube further includes a cover 5, where the cover 5 covers one end of the tube 1, and the cover 5 is detachably connected to the tube 1, so as to avoid leakage of tissue from the other side of the mounting plate 31 away from the filter screen 11 during the dissociation process. Specifically, the cover body 5 comprises an end 51 and an extending part 52 which are connected with each other, the outer diameter of the end 51 is larger than that of the extending part 52, the extending part 52 is blocked at one side of the pipe body 1, and the end 51 is blocked at one end of the pipe body 1, so that the sealing effect is improved, and the leakage of internal tissues is avoided.

Optionally, as shown in fig. 1, the first grinding body 2 further includes a second connecting shaft 23, the second connecting shaft 23 is disposed outside the housing 21, the second connecting shaft 23 penetrates through the cover 5, and the external power output device is connected with the second connecting shaft 23 to enable the first grinding body 2 to rotate, so that the first grinding body 2 and the second grinding body 3 relatively rotate to grind; by providing the second connecting shaft 23, it is arranged outside the casing 21, passes through the cover 5 and then is connected with an external power output device, and the structure is compact. Specifically, the first connecting shaft 22 and the second connecting shaft 23 are coaxially disposed, reducing the overall circumferential structure.

In this embodiment, as shown in fig. 1 and fig. 2, the filter screen 11 is mounted on the pipe body 1, or in other embodiments, the filter screen 11 may be mounted on a side of the support frame 4 away from the second grinding body 3, which is not limited, and may be set according to actual situations; the filter screen 11 may be of an existing structure, and will not be described in detail.

Specifically, the inner side wall of the pipe body 1 is also provided with a supporting protrusion 12 for supporting the supporting frame 4, so that the supporting frame 4 is prevented from slipping towards one side provided with the filter screen 11 to form axial limit.

Alternatively, as shown in fig. 1 to 4, an extension rod 33 is provided on the side of the mounting plate 31 facing away from the grinding bit 32, and a stopper 34 is provided on an end 51 of the extension rod 33; the periphery of the support frame 4 is provided with a first groove 42 and a second groove 43 which are communicated with each other, and the groove depth of the second groove 43 along the radial direction of the support frame 4 is larger than the groove depth of the first groove 42 along the support frame 4; the extension rod 33 can be worn to locate between the hole of wearing that first recess 42 and body 1 formed, stopper 34 sliding connection in second recess 43, and stopper 34 can be stopped to the cell wall of first recess 42, and in mounting panel 31 and support frame 4 separation in-process for the axial spacing of second grinding body 3, it is deviate from body 1 to avoid first grinding body 2 and second grinding body 3 to slide.

It is to be understood that the above examples of the present invention are provided for clarity of illustration only and are not limiting of the embodiments of the present invention. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. Any modification, equivalent replacement, improvement, etc. which come within the spirit and principles of the invention are desired to be protected by the following claims.

Claims

1. The tissue dissociation tube is characterized by comprising a tube body (1), wherein the tube body (1) is internally provided with:

a first polishing body (2) having a polishing groove;

the second grinding body (3) comprises a mounting plate (31) and a grinding head (32), wherein the mounting plate (31) is arranged on one side of a notch of the grinding groove, the mounting plate (31) and the grinding groove form a containing space (31A), the grinding head (32) is arranged in the containing space (31A), a grinding space for containing tissues to be ground is formed between the grinding head (32) and the grinding groove, the first grinding body (2) is rotationally connected with the second grinding body (3), one of the first grinding body (2) and the second grinding body (3) can be driven by an external power output device to rotate so as to grind the tissues to be ground, and a plurality of first through grooves (311) are formed in the periphery of the mounting plate (31);

the support frame (4) is provided with a plurality of second through grooves (41) in the circumferential direction, the support frame (4) can be abutted or separated on one side of the mounting plate (31) away from the grinding head (32), and the plurality of first through grooves (311) and the plurality of second through grooves (41) are staggered;

the filter screen (11) is arranged on one side of the supporting frame (4) away from the second grinding body (3);

the first grinding body comprises a shell (21) and a first connecting shaft (22) arranged in the shell (21), the first connecting shaft (22) is rotationally connected with the grinding head (32), and the grinding groove is formed in the shell (21); the pipe body (1) is a transparent body, the shell (21) comprises a shell bottom plate (211) and a half-ring plate (212) which are connected with each other, and the shell bottom plate (211) is arranged on the first connecting shaft (22); the semi-ring plate (212) is provided with a plurality of groove bodies (2121) continuously along the circumferential direction, the depths of the groove bodies (2121) are sequentially increased, and the second grinding body (3) can rotate relative to the first grinding body (2) from the side with the larger depth of the groove bodies (2121) to the side with the smaller depth of the groove bodies (2121);

the periphery of the grinding head (32) is provided with a plurality of first cutting blades (321) which are arranged at intervals;

the first cutting blade (321) is in a tooth-shaped structure.

2. The tissue dissociation tube of claim 1, further comprising a cover (5), the cover (5) being arranged to cover one end of the tube (1), the cover (5) being detachably connected to the tube (1).

3. The tissue dissociation tube of claim 2, wherein the first abrasive body (2) further comprises a second connection shaft (23), the second connection shaft (23) being arranged outside the housing (21), the second connection shaft (23) being threaded through the cover (5), the external power take-off being connected to the second connection shaft (23).

4. A tissue dissociation tube as claimed in any one of claims 1 to 3, characterised in that the screen (11) is mounted on the tube body (1) or the support frame (4).

5. A tissue dissociation tube as claimed in any one of claims 1-3, characterised in that the side of the mounting plate (31) facing away from the grinding head (32) is provided with an extension rod (33), the end (51) of the extension rod (33) being provided with a stop (34);

the periphery of the support frame (4) is provided with a first groove (42) and a second groove (43) which are communicated with each other, and the groove depth of the second groove (43) along the radial direction of the support frame (4) is larger than the groove depth of the first groove (42) along the support frame (4);

the extension rod (33) can be arranged between the first groove (42) and a penetrating hole formed by the pipe body (1) in a penetrating mode, the limiting block (34) is connected in the second groove (43) in a sliding mode, and the limiting block (34) can be blocked by the groove wall of the first groove (42).