CN214115521U - Experimental device for extraction of primary cells of solid tissue - Google Patents
Experimental device for extraction of primary cells of solid tissue Download PDFInfo
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- CN214115521U CN214115521U CN202022399527.XU CN202022399527U CN214115521U CN 214115521 U CN214115521 U CN 214115521U CN 202022399527 U CN202022399527 U CN 202022399527U CN 214115521 U CN214115521 U CN 214115521U
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Abstract
The utility model discloses an experimental device for extracting primary cells from solid tissues, which comprises a sleeve, a tube cover, a stirring cover and a filter cylinder, wherein the tube cover can cover an opening at the top of a sealing sleeve; the stirring cover comprises a cover body, a stirring shaft and a handle; the cover body can cover the opening at the top of the sealing sleeve, and a rotating motor is arranged in the cover body; the top of the stirring shaft is detachably and fixedly connected with a rotating shaft of the rotating motor, and stirring blades are arranged on the stirring shaft; the handle and the cover body are connected into a whole, and a power supply is arranged in the handle; the filter cartridge is detachably arranged above the inner side of the sleeve; when the cover body of the stirring cover covers the opening at the top of the sealing sleeve, the stirring shaft extends into the filter cylinder. The objects in the filter cartridge can be cut and crushed; the problem of huge tissue processing work load in the process of extracting the primary cells of the entity tissue at present is solved, the steps of extracting the primary cells are simplified, time is saved, the pollution probability is reduced, the whole extraction process is easier to operate, and the device is portable and easy to operate.
Description
Technical Field
The utility model belongs to medical auxiliary assembly, concretely relates to experimental apparatus of primary cell extraction of entity tissue.
Background
At present, the extraction of primary cells of basic experimental entity tissues needs to shear the entity tissues by sterile scissors, and the amount of primary cells extracted from a small amount of entity tissues is small, so that larger entity tissues need to be processed frequently in order to obtain enough primary cells. Manual cutting greatly increases the workload and increases the risk of primary cell contamination after work fatigue. And the subsequent treatment steps after the solid tissues are cut into pieces are complicated and trivial, and the required experimental instruments are separated, so that one of the two instruments is often missed, and the experiment is delayed.
SUMMERY OF THE UTILITY MODEL
Problem to prior art exists, the utility model provides an experimental apparatus of primary cell extraction of entity tissue solves the huge problem of the primary cell extraction in-process tissue handling work load of present entity tissue, simplifies primary cell extraction step, saves time, reduces the pollution probability, makes whole extraction process change the operation, and the portable easy operation of this device.
The technical scheme of the utility model is that: an experimental device for extracting primary cells of solid tissues comprises a sleeve, wherein the sleeve is of a cup-shaped structure with a bottom and without a top;
the sealing sleeve comprises a tube cover, wherein the tube cover can cover the top opening of the sealing sleeve;
the stirring device comprises a stirring cover, wherein the stirring cover comprises a cover body, a stirring shaft and a handle; the cover body can cover the opening at the top of the sealing sleeve, and a rotating motor is arranged in the cover body; the top of the stirring shaft is detachably and fixedly connected with a rotating shaft of the rotating motor, and stirring blades are arranged on the stirring shaft; the handle and the cover body are connected into a whole, and a power supply is arranged in the handle;
the filter cartridge is detachably arranged above the inner side of the sleeve; when the cover body of the stirring cover covers the opening at the top of the sealing sleeve, the stirring shaft extends into the filter cylinder. The objects in the filter cartridge can be cut and crushed.
Further, the device also comprises a filter funnel, wherein the filter funnel comprises a conical hopper in the middle; a connecting sleeve is arranged at the large opening end of the conical hopper and can be fixedly connected with the opening at the top of the sleeve; a filter screen is arranged between the connecting sleeve and the large opening end of the conical hopper; and a filtering outlet is arranged at the small-opening end of the conical hopper. The matched filter funnel is adopted, the filter funnel can be arranged between the cell digestion tubes for cell culture after cell digestion, and the operation is convenient.
Further, the top of the inner side of the sleeve is provided with a radially contracted step surface, the top of the filter cylinder radially protrudes with a cap peak, and the cap peak can be matched with and placed on the step surface. The installation is convenient.
Further, the outer surface of the top of the sleeve is provided with an external thread, the inner surface of the cover body of the stirring cover is provided with a matched internal thread, and the cover body of the stirring cover is hermetically connected with the sleeve through the external thread and the internal thread.
Further, the outer surface of the top of the sleeve is provided with external threads, the inner surface of the pipe cover is provided with matched internal threads, and the pipe cover is in sealing connection with the sleeve through the external threads and the internal threads.
Furthermore, the outer surface of the top of the sleeve is provided with an external thread, the inner surface of the connecting sleeve of the filtering funnel is provided with a matched internal thread, and the connecting sleeve of the filtering funnel is hermetically connected with the sleeve through the external thread and the internal thread.
Furthermore, the filter screen adopts metal molybdenum with the specification of 200 molybdenum or 100 molybdenum.
The utility model has the advantages that: a stirring shaft with blades is adopted to carry out efficient cutting in the metal sleeve, so that the solid tissue is rapidly crushed; the filter cartridge is adopted, and the mesh screen on the filter cartridge can enable the tissue meeting the requirement to sink, so that excessive crushing and cell activity damage to the tissue are avoided; then directly carrying out digestive enzyme digestion neutralization in a metal sleeve; then filtering out solid tissues by using a matched filter funnel, centrifugally collecting cells, and inoculating and plating for culture. The whole device not only simplifies the experimental steps, but also is portable and easy to operate.
Drawings
FIG. 1 is a schematic structural view of a stirring cover;
FIG. 2 is a schematic structural view of a bushing;
FIG. 3 is a cross-sectional view of the cannula;
FIG. 4 is a schematic structural view of a filter cartridge;
FIG. 5 is a cross-sectional view of the assembled filter cartridge and sleeve;
FIG. 6 is a schematic view of the assembled structure of the sleeve, filter cartridge and agitator cap;
FIG. 7 is a schematic view of the structure of a filter funnel;
fig. 8 is a schematic view of the assembled filter funnel and sleeve.
In the figure: 1 is the sleeve pipe, 2 is the stirring lid, 3 is the cartridge filter, 4 is filtering funnel, 11 is the step face, 12 is the external screw thread, 21 is the lid, 22 is the (mixing) shaft, 23 is the handle, 31 is the brim of a hat, 41 is the adapter sleeve, 42 is the toper fill, 43 is filtering outlet, 44 is the filter screen.
Detailed Description
The present invention will be further described with reference to the accompanying drawings.
An experimental device for extracting primary cells from solid tissues comprises a cannula 1 shown in fig. 2, a tube cover, a stirring cover 2 shown in fig. 1, a filter cartridge 3 shown in fig. 4 and a filter funnel 4 shown in fig. 7.
The sleeve 1 is a cup-shaped structure with a bottom and no top. The outer surface of the top of the sleeve 1 is provided with external threads 12. The inner surface of the pipe cover is provided with matched internal threads, and the pipe cover is connected with the sleeve pipe 1 through the external threads 12 and the internal threads in a sealing mode.
The stirring cover 2 comprises a cover body 21, a stirring shaft 22 and a handle 23. The inner surface of the cover body 21 of the stirring cover 2 is provided with matched internal threads, and the cover body 21 of the stirring cover 2 is hermetically connected with the sleeve 1 through the external threads 12 and the internal threads. A rotating electric machine is provided in the lid body 21. The top of the stirring shaft 22 is fixedly connected with a rotating shaft of the rotating motor in a detachable mode, and stirring blades are arranged on the stirring shaft 22. The handle 23 and the cover body 21 are connected into a whole, and a power supply is arranged in the handle 23.
The filter cartridge 3 is detachably arranged above the inner side of the sleeve 1. When the cover body of the stirring cover covers the opening at the top of the sealing sleeve, the stirring shaft extends into the filter cylinder. The objects in the filter cartridge can be cut and crushed.
The filter funnel 4 comprises a conical funnel 42 in the middle. The connecting sleeve 41 is arranged at the large opening end of the conical hopper 42, the inner surface of the connecting sleeve 41 is provided with matched internal threads, and the connecting sleeve 41 of the filtering funnel 4 is hermetically connected with the sleeve 1 through the external threads 12 and the internal threads. A 200 molybdenum or 100 molybdenum filter screen 44 is arranged between the connecting sleeve 41 and the large opening end of the conical hopper 42. The small end of the conical hopper 42 is provided with a filtering outlet 43. The matched filter funnel is adopted, the filter funnel can be arranged between the cell digestion tubes for cell culture after cell digestion, and the operation is convenient.
In a preferred embodiment, as shown in fig. 2 and 3, a radially contracted step surface 11 is provided at the top of the inner side of the sleeve 1, a cap visor 31 protrudes radially from the top of the filter cartridge 3, and the cap visor 31 can be fittingly placed on the step surface 11. The installation is convenient.
When in use, the following steps are adopted:
step one, carrying out irradiation sterilization on a handle with a power supply and a cover body with a rotating motor of the whole primary cell extraction device. The stirring shaft with stirring blades and a 50ml sleeve were sterilized by a high pressure steam sterilizer.
Step two, placing the sterilized solid tissue into a filter cartridge, placing the filter cartridge into a sleeve (enough culture medium is added into the sleeve) according to the description of fig. 5, then covering the top opening of the sealing sleeve with a cover body of a stirring cover as shown in fig. 6, and extending a stirring shaft into the filter cartridge. And starting a switch, and selecting a proper rotating speed to rapidly cut and crush the tissues in the filter cartridge.
Step three, after the mixture is crushed to a proper degree, the tissues can sink to the bottom of the sleeve, and after the mixture is completely crushed, the sleeve is centrifuged and the supernatant is discarded; adding digestive enzyme to digest according to the requirement of the extracted cells; at this point the tube was capped and placed in a 37 degree water bath for digestion.
And step four, opening a tube cover, adding a culture medium with serum for neutralization, covering a filter funnel on an opening at the top of a sealing sleeve as shown in fig. 8, filtering to a cell culture 50ml centrifuge tube by using a filter screen, and performing inoculation culture on the resuspended cells after centrifugation (the metal sleeve has the same specification as the conventional cell culture 50ml centrifuge tube, and is convenient to use a conventional centrifuge).
The primary cell extraction device adopts a stirring shaft with blades to perform efficient cutting in a metal sleeve, so that the rapid crushing of solid tissues is realized; the filter cartridge is adopted, and the mesh screen on the filter cartridge can enable the tissue meeting the requirement to sink, so that excessive crushing and cell activity damage to the tissue are avoided; then directly carrying out digestive enzyme digestion neutralization in a metal sleeve; then filtering out solid tissues by using a matched filter funnel, centrifugally collecting cells, and inoculating and plating for culture. The whole device not only simplifies the experimental steps, but also is portable and easy to operate.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (7)
1. An experimental device for extracting primary cells of solid tissues is characterized in that: the sleeve comprises a sleeve (1), wherein the sleeve (1) is of a cup-shaped structure with a bottom and without a top;
comprises a tube cover which can cover the top opening of the sealing sleeve (1);
the stirring device comprises a stirring cover (2), wherein the stirring cover (2) comprises a cover body (21), a stirring shaft (22) and a handle (23); the cover body (21) can cover the opening at the top of the sealing sleeve (1), and a rotating motor is arranged in the cover body (21); the top of the stirring shaft (22) is detachably and fixedly connected with a rotating shaft of a rotating motor, and stirring blades are arranged on the stirring shaft (22); the handle (23) is connected with the cover body (21) into a whole, and a power supply is arranged in the handle (23);
the filter comprises a filter cartridge (3), wherein the filter cartridge (3) is detachably arranged above the inner side of a sleeve (1); when the cover body of the stirring cover covers the opening at the top of the sealing sleeve, the stirring shaft extends into the filter cylinder.
2. The experimental apparatus for extracting primary cells from solid tissue according to claim 1, wherein: the filter device also comprises a filter funnel (4), wherein the filter funnel (4) comprises a conical hopper (42) in the middle; a connecting sleeve (41) is arranged at the large opening end of the conical hopper (42), and the connecting sleeve (41) can be fixedly connected with an opening at the top of the sleeve (1); a filter screen (44) is arranged between the connecting sleeve (41) and the large opening end of the conical hopper (42); and a filtering outlet (43) is formed at the small-opening end of the conical hopper (42).
3. The experimental apparatus for extracting primary cells from solid tissue according to claim 1, wherein: the novel filter cartridge is characterized in that a radially contracted step surface (11) is arranged at the top of the inner side of the sleeve (1), a cap peak (31) protrudes from the top of the filter cartridge (3) in the radial direction, and the cap peak (31) can be matched with the step surface (11) to be placed on.
4. The experimental apparatus for extracting primary cells from solid tissue according to claim 1, wherein: the outer surface of the top of the sleeve (1) is provided with an external thread (12), the inner surface of the cover body (21) of the stirring cover (2) is provided with a matched internal thread, and the cover body (21) of the stirring cover (2) is in sealing connection with the sleeve (1) through the external thread (12) and the internal thread.
5. The experimental apparatus for extracting primary cells from solid tissue according to claim 1, wherein: the outer surface of the top of the sleeve (1) is provided with an external thread (12), the inner surface of the pipe cover is provided with a matched internal thread, and the pipe cover is hermetically connected with the sleeve (1) through the external thread (12) and the internal thread.
6. The experimental apparatus for extracting primary cells from solid tissue according to claim 2, wherein: the utility model discloses a filter funnel, including sleeve pipe (1), adapter sleeve (41) internal surface of filter funnel (4) is provided with external screw thread (12), the adapter sleeve (41) of filter funnel (4) and sleeve pipe (1) between be connected through external screw thread (12) and internal thread sealing.
7. The experimental apparatus for extracting primary cells from solid tissue according to claim 2, wherein: the filter screen (44) adopts metal molybdenum with the specification of 200 meshes or 100 meshes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022399527.XU CN214115521U (en) | 2020-10-26 | 2020-10-26 | Experimental device for extraction of primary cells of solid tissue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022399527.XU CN214115521U (en) | 2020-10-26 | 2020-10-26 | Experimental device for extraction of primary cells of solid tissue |
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| Publication Number | Publication Date |
|---|---|
| CN214115521U true CN214115521U (en) | 2021-09-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022399527.XU Active CN214115521U (en) | 2020-10-26 | 2020-10-26 | Experimental device for extraction of primary cells of solid tissue |
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| CN (1) | CN214115521U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114958720A (en) * | 2022-06-07 | 2022-08-30 | 王翠玲 | Cell separation method |
-
2020
- 2020-10-26 CN CN202022399527.XU patent/CN214115521U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114958720A (en) * | 2022-06-07 | 2022-08-30 | 王翠玲 | Cell separation method |
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