CN113924359A - 通过番茄次碱处理促进干细胞分化为成熟心肌细胞的方法 - Google Patents

通过番茄次碱处理促进干细胞分化为成熟心肌细胞的方法 Download PDF

Info

Publication number
CN113924359A
CN113924359A CN202080040203.7A CN202080040203A CN113924359A CN 113924359 A CN113924359 A CN 113924359A CN 202080040203 A CN202080040203 A CN 202080040203A CN 113924359 A CN113924359 A CN 113924359A
Authority
CN
China
Prior art keywords
cardiomyocytes
stem cells
solanesoximine
differentiated
composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202080040203.7A
Other languages
English (en)
Chinese (zh)
Inventor
金载皓
金艺瑟
尹停媛
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hessel Technology Co ltd
Original Assignee
Hessel Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hessel Technology Co ltd filed Critical Hessel Technology Co ltd
Publication of CN113924359A publication Critical patent/CN113924359A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657Cardiomyocytes; Heart cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/34Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00Drugs for disorders of the cardiovascular system
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40Regulators of development
    • C12N2501/415Wnt; Frizzeled
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70Enzymes
    • C12N2501/72Transferases (EC 2.)
    • C12N2501/727Kinases (EC 2.7.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999Small molecules not provided for elsewhere
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cardiology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Vascular Medicine (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Epidemiology (AREA)
  • Rheumatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN202080040203.7A 2019-05-28 2020-04-09 通过番茄次碱处理促进干细胞分化为成熟心肌细胞的方法 Pending CN113924359A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR10-2019-0062579 2019-05-28
KR1020190062579A KR102167257B1 (ko) 2019-05-28 2019-05-28 토마티딘 처리를 통한 줄기세포의 성숙화된 심근세포로의 분화유도 촉진 방법
PCT/KR2020/004841 WO2020242045A1 (fr) 2019-05-28 2020-04-09 Procédé pour favoriser la différenciation de cellules souches en cardiomyocytes matures par un traitement avec de la tomatidine

Publications (1)

Publication Number Publication Date
CN113924359A true CN113924359A (zh) 2022-01-11

Family

ID=73042827

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080040203.7A Pending CN113924359A (zh) 2019-05-28 2020-04-09 通过番茄次碱处理促进干细胞分化为成熟心肌细胞的方法

Country Status (3)

Country Link
KR (1) KR102167257B1 (fr)
CN (1) CN113924359A (fr)
WO (1) WO2020242045A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20230152192A (ko) 2022-04-21 2023-11-03 주식회사 스마트셀랩 커큐민에 의한 줄기세포의 심근전구세포로의 유도 및 심근세포로의 분화

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160177267A1 (en) * 2014-12-18 2016-06-23 President And Fellows Of Harvard College Serum-free in vitro directed differentiation protocol for generating stem cell-derived beta cells and uses thereof
WO2018170280A1 (fr) * 2017-03-15 2018-09-20 University Of Washington Méthodes et compositions pour améliorer la maturation et la prise de greffe de cardiomyocytes

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4950660B2 (ja) * 2003-06-27 2012-06-13 エチコン、インコーポレイテッド 分娩後由来細胞を使用する眼組織の修復および再生
US10913929B2 (en) * 2009-10-13 2021-02-09 Stemcell Technologies Inc. Method of differentiating stem cells
CN106414718A (zh) * 2013-06-11 2017-02-15 哈佛学院校长同事会 SC-β细胞以及用于产生其的组合物和方法
KR102604999B1 (ko) * 2014-09-08 2023-11-23 고쿠리쓰 겐큐 가이하쓰 호징 리가가쿠 겐큐소 소뇌 선구체 조직의 제조 방법

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160177267A1 (en) * 2014-12-18 2016-06-23 President And Fellows Of Harvard College Serum-free in vitro directed differentiation protocol for generating stem cell-derived beta cells and uses thereof
WO2018170280A1 (fr) * 2017-03-15 2018-09-20 University Of Washington Méthodes et compositions pour améliorer la maturation et la prise de greffe de cardiomyocytes

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
PARIKH SS ET AL.: "hyroid and Glucocorticoid Hormones Promote Functional T-Tubule Development in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes", 《CIRC RES.》, 8 December 2017 (2017-12-08), pages 1323 - 1330 *
SILVA DOS SANTOS DET AL.: "Embryonic stem cell-derived cardiomyocytes for the treatment of doxorubicin-induced cardiomyopathy", 《STEM CELL RES THER.》, vol. 9, no. 1, 5 February 2018 (2018-02-05) *
傅继东: "分化发育早期心肌细胞钙稳态的调控——应用胚胎干细胞向心肌细胞分化体外模型的基础研究", 《中国优秀博硕士学位论文全文数据库 (博士)基础科学辑》, 15 October 2006 (2006-10-15) *

Also Published As

Publication number Publication date
WO2020242045A1 (fr) 2020-12-03
KR102167257B1 (ko) 2020-10-19

Similar Documents

Publication Publication Date Title
JP7386290B2 (ja) ヒト心室前駆細胞の生着のための遺伝子マーカー
Rupert et al. IGF1 and NRG1 enhance proliferation, metabolic maturity, and the force‐frequency response in hESC‐derived engineered cardiac tissues
TWI720333B (zh) 多能性幹細胞的製備方法、使用該製備方法而製備出多能性幹細胞、改善劑、以及該多能性幹細胞之分化誘導方法
CN106459922B (zh) Cd82阳性心肌前体细胞
AU2017314870A1 (en) Cardiomyocyte maturation
JP7275109B2 (ja) ニューロピリン-1(nrp1)のヒト心室前駆細胞を単離するための細胞表面マーカーとしての使用
EP3183337B1 (fr) Utilisation de jagged 1/frizzled 4 à titre de marqueurs de surface cellulaire pour isoler des cellules progénitrices ventriculaires cardiaques humaines
CN105518125A (zh) 用于产生心外膜细胞的方法和组合物
WO2013063305A2 (fr) Différenciation dirigée en cardiomyocytes de cellules souches
KR101562366B1 (ko) 메타크릴레이트화된 젤라틴을 포함하는, 심근세포분화 유도용 세포 지지체
JP2024037905A (ja) 心筋細胞を生成及び増殖させるためのwntアゴニスト、並びに生理活性脂質を用いた試薬及び方法
WO2015081094A1 (fr) Cellules progénitrices cardiaques et leurs procédés d'utilisation
US9155765B2 (en) Establishment of patient- or person-specific cardiac myocyte cell lines from human induced pluripotent stem cells (iPSCs)
CN113924359A (zh) 通过番茄次碱处理促进干细胞分化为成熟心肌细胞的方法
KR101539132B1 (ko) 유전자 도입 없이 저분자성 물질을 이용하여 체세포로부터 심근세포를 유도하는 방법
US7534607B1 (en) Method of producing cardiomyocytes from mesenchymal stem cells
Di Felice et al. OPLA scaffold, collagen I, and horse serum induce a higher degree of myogenic differentiation of adult rat cardiac stem cells
Liu et al. A net-shaped multicellular formation facilitates the maturation of hPSC-derived cardiomyocytes through mechanical and electrophysiological stimuli
JP2016077159A (ja) 心筋細胞の製造方法、心室型心筋細胞及びその製造方法、並びにスクリーニング方法
JP2010246476A (ja) 心筋細胞分化誘導促進剤およびその使用方法
WO2017177050A1 (fr) Reprogrammation directe de cellules somatiques en cellules myogènes
Xue et al. Angiotensin II promotes differentiation of mouse c-kit-positive cardiac stem cells into pacemaker-like cells
JPWO2005116194A1 (ja) 試験管内筋繊維形成のための筋芽細胞又は筋芽細胞様細胞培養法
Feige Molecular Regulation of Satellite Cell Fate
Hoes Studying cardiac diseases using human stem cell-derived cardiomyocytes

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination