CN113893305A

CN113893305A - Traditional Chinese medicine composition for preventing and treating salpingitis of laying hens as well as preparation process and application thereof

Info

Publication number: CN113893305A
Application number: CN202111428854.6A
Authority: CN
Inventors: 王德云; 杨阳; 武志勇; 朱少武; 刘振广
Original assignee: Nanjing Agricultural University
Current assignee: Nanjing Agricultural University
Priority date: 2021-11-29
Filing date: 2021-11-29
Publication date: 2022-01-07
Anticipated expiration: 2041-11-29
Also published as: CN113893305B

Abstract

The invention discloses a traditional Chinese medicine composition for preventing and treating salpingitis of laying hens and a preparation process and application thereof, wherein active ingredients of the traditional Chinese medicine composition comprise substances prepared according to the following formula; the formula comprises the following traditional Chinese medicine components: herba Leonuri, herba Polygoni Capitati, rhizoma Smilacis chinensis, fructus Cnidii, herba plantaginis, and Concha Ostreae. The composition provided by the invention has the effects of clearing away heat and toxic materials, inducing diuresis with bland drugs and resisting inflammation, can be used for remarkably controlling salpingitis of laying hens in laying hen breeding and improving the quality of the laying hens, has a definite curative effect, and is suitable for antibiotic reduction in nonreactive breeding and livestock husbandry industries. Under the background that the use of antibiotics is limited and the antibiotics are controlled to be remained in animal products by the current national policy, the culture benefit can be improved, the egg quality is improved, and the method has huge market prospect and has obvious economic benefit and social benefit.

Description

Traditional Chinese medicine composition for preventing and treating salpingitis of laying hens as well as preparation process and application thereof

Technical Field

The invention relates to the technical field of veterinary drugs, and in particular relates to a traditional Chinese medicine composition for preventing and treating salpingitis of laying hens, and a preparation process and application thereof.

Background

In the seventy-eight years of the last century, the laying hen industry in China has been vigorously developed, and the egg yield has been steadily at the first in the world since 1985. With the development of the laying hen technology, the specialized, facility, fine breed and marketized laying hen industries are basically completed, and the industry division system is relatively perfect. At present, the egg yield of China is about 2450 million tons. Eggs are indispensable protein food in daily life of people, the breeding economic benefit and the quality safety of the eggs are the most concerned problems of farmers and consumers respectively, and the key to improving the breeding benefit is how to improve the production performance of laying hens and the quality of the eggs. A large amount of antibiotics are frequently used in the disease prevention and treatment process of laying hens, so that the problems of standard exceeding of veterinary drug residues, drug resistance of bacteria, quality safety of livestock and poultry products and the like are easily caused, and serious risk potential hazards are caused to food quality safety, public health safety and ecological safety. The 'banning' of the feed is a great trend, and the feed is to be comprehensively 'banned' by 2020 in China.

Salpingitis is a common disease harmful to laying hens, and often causes the laying rate of the laying hens to be reduced, the number of defective eggs such as soft-shell eggs, blood spot eggs and eggshell eggs is increased, and the breeding profit is seriously influenced. Salpingitis is often closely related to dampness, heat and toxicity, and often causes phlegm stagnation, qi stagnation and blood stasis, and inhibits yang qi from running after long-term development, and shows symptoms of dampness syndrome or lower reproductive function and the like. The traditional Chinese medicine has the advantages of rich resources, low toxicity, low drug residue and the like, and becomes one of the best choices of 'replacing resistance' along with the comprehensive 'banning resistance' of the feed. China has a huge breeding industry market and rich traditional Chinese medicine resources, has a long-history traditional Chinese medicine research history, and has great development prospect along with the continuous progress of scientific technology. Therefore, the invention provides a traditional Chinese medicine composition for preventing and treating salpingitis of laying hens and a preparation process and application thereof, so as to effectively solve the problems of salpingitis and egg laying of the laying hens.

Disclosure of Invention

The purpose of the invention is as follows: the invention aims to solve the technical problem of providing a traditional Chinese medicine composition for preventing and treating salpingitis of laying hens aiming at the defects of the prior art.

The technical problem to be solved by the invention is to provide a preparation containing the traditional Chinese medicine composition.

The technical problem to be solved by the invention is to provide a preparation method of the traditional Chinese medicine composition.

The invention finally aims to solve the technical problem of providing the application of the traditional Chinese medicine composition and the preparation.

The invention idea is as follows: the compound Chinese medicine preparation is prepared with Chinese medicinal materials, and has the functions of clearing away heat and toxic material, promoting diuresis, promoting blood circulation to disperse blood clots, strengthening liver and invigorating kidney, and can prevent salpingitis caused by downward flow of heat and damp, improve microcirculation and rheological property of blood, promote the absorption and elimination of inflammation and necrosis tissue, reduce tissue proliferation and adhesion, speed the repair of damaged oviduct and ovary, strengthen liver and invigorate kidney, delay egg laying rate and raise egg laying rate.

In order to solve the first technical problem, the invention discloses a traditional Chinese medicine composition for preventing and treating salpingitis of laying hens, wherein active ingredients of the traditional Chinese medicine composition comprise substances prepared according to the following formula; the formula comprises the following traditional Chinese medicine components: herba Leonuri, herba Polygoni Capitati, rhizoma Smilacis chinensis, fructus Cnidii, herba plantaginis, and Concha Ostreae. In the formula, the motherwort herb is used as a monarch drug for inducing diuresis to alleviate edema, promoting blood circulation and removing blood stasis; the polygonum capitatum has the effects of removing dampness by light infiltration, and inducing diuresis for treating stranguria; the smilax china is used as a ministerial drug together with the two drugs of promoting diuresis, removing turbidity, detoxifying and dissipating blood stasis; fructus Cnidii has effects of eliminating dampness, dispelling pathogenic wind, warming yang and invigorating kidney; the plantain herb has the functions of promoting diuresis, eliminating dampness, clearing away heat and toxic materials; the oyster three medicines for calming the liver, subduing yang and softening and resolving hard mass are used as assistant and guiding medicines. The combination of the medicines can effectively solve the problems of egg laying function reduction and eggshell quality reduction caused by salpingitis and kidney yang deficiency caused by the damp-heat syndrome of the laying hens, and simultaneously, the organic calcium and phosphorus in the oysters can improve the calcium and phosphorus balance of the laying hens.

Wherein the components comprise, by mass, 20-30 parts of motherwort, 20-30 parts of polygonum capitatum, 20-30 parts of smilax china, 20-30 parts of common cnidium fruit, 20-30 parts of plantain herb and 80-120 parts of oyster; preferably, the components comprise, by mass, 20 parts of motherwort, 20 parts of polygonum capitatum, 20 parts of smilax china, 20 parts of common cnidium fruit, 20 parts of plantain herb and 80 parts of oyster; further preferably, the components comprise 20g of motherwort, 20g of polygonum capitatum, 20g of smilax china, 20g of common cnidium fruit, 20g of plantain herb and 80g of oyster.

In order to solve the second technical problem, the invention discloses a preparation for preventing and treating salpingitis of laying hens, which comprises the traditional Chinese medicine composition.

Wherein, the preparation is in a unit dosage form suitable for nasal mucosa, oral mucosa, sublingual administration, oral administration, skin administration, injection administration or pulmonary inhalation.

In order to solve the third technical problem, the invention discloses a preparation method of the traditional Chinese medicine composition, which comprises the following steps:

s1: according to the formula, crushing other components except oysters, performing reflux extraction by using an acidic alcohol solution, filtering to obtain filtrate, recovering ethanol, and adjusting the pH value to 6.5-7.5 by using sodium hydroxide for later use; decocting the residue with water for 2 times, filtering, and mixing the three filtrates; the pH value is preferably 7.0;

s2: concentrating the filtrate to obtain concentrated solution, adding silicon dioxide for adsorption and drying;

s3: and (8) mixing the dried product of S2 with oyster to obtain the product.

In step S1, the pulverization is to a coarse powder of about 2 mm.

In step S1, the extraction efficiency of leonurine hydrochloride can be effectively improved through reflux extraction of acidic alcohol solution, the damage to the effective components of the traditional Chinese medicine is reduced, and the product effect is enhanced.

In step S1, the acidic alcohol solution is a mixed solution of hydrochloric acid and ethanol.

Wherein the concentration of the hydrochloric acid is 0.02-0.08mol/L, and preferably 0.05 mol/L.

Wherein the concentration of the ethanol is 40-60%, and preferably 50%.

In step S1, the mass ratio of the solvent to the raw material is (4-8):1, preferably 6: 1.

In step S1, the reflux extraction time is 1-3h, preferably 2 h.

In the step S1, the process also comprises decoction, other water-soluble active ingredients in the decoction dregs can be completely extracted through the decoction, and all effects of the medicinal materials are effectively exerted; specifically, according to the formula, pulverizing the components except Concha Ostreae, reflux-extracting with acidic alcohol solution, filtering, decocting the obtained residue for several times, filtering again, and mixing the filtrates; concentrating the obtained filtrate to obtain concentrated solution; the temperature of the reflux extraction is 80 ℃; the temperature of the decoction is 100 ℃.

Wherein the number of times of decoction is 1-3, preferably 2.

Wherein the decoction is carried out by adding water, and the mass ratio of the water to the raw materials (the components except the oyster) is (4-8) to 1, preferably 6 to 1.

Wherein the decoction time is 1-3h, preferably 2h, more preferably 2h for the first decoction and 1.5h for the second decoction.

In step S2, the concentration is carried out until the density is 1.05-1.08g/cm³。

In step S2, the concentration is performed by using a triple-effect concentrator, which can effectively shorten the concentration time and reduce the loss of effective components.

Wherein, the vacuum degree and the evaporation temperature of each effect in the triple-effect concentrator are as follows: first effect is-0.04-0.07 MPa, 85 ℃; second effect-0.06-0.08 MPa, 75 deg.C, third effect-0.07-0.10 MPa, 65 deg.C.

In step S2, the drying is performed by adsorption with particulate silica and paddle-screw drying. The product has good free-running property, the volume weight is close to that of the feed raw materials of corn flour and soybean meal, and the adding uniformity in the feed is good. The paddle spiral drying method has the advantages of large heating area, short drying time and low energy consumption.

Wherein the mass ratio of the concentrated solution to the silicon dioxide is 1 (0.5-3.5), preferably 1: 2.

In step S3, the oysters are crushed and sieved by 60-80 meshes.

In the step S3, the oysters are added, so that the effects of warming the kidney, supporting yang, dissipating stagnation and softening hardness of the oysters can be exerted, organic calcium and phosphorus in the oysters can be utilized by laying hens, and the quality of eggshells is improved.

Preferably, the preparation method comprises the steps of weighing 20-30 parts of motherwort, 20-30 parts of polygonum capitatum, 20-30 parts of smilax china, 20-30 parts of common cnidium fruit, 20-30 parts of plantain herb and 80-120 parts of oyster according to the weight proportion; pulverizing the above materials except Concha Ostreae into coarse powder of about 2 mm; adding 0.05mol/L diluted hydrochloric acid 50% ethanol solution, soaking for 60 min, reflux extracting for 2 hr, with the mass ratio of solvent to raw materials being 6:1, extracting the filtrate, recovering ethanol, and adjusting pH to 7.0 with sodium hydroxide for use; decocting the residue with water for 2 times, wherein the weight ratio of water to the raw materials is 6:1, and the decocting time is 2 hr and 1.5 hr respectively; mixing filtrates, and concentrating to density of 1.05-1.08; adding silicon dioxide into the concentrated solution, mixing, drying at 60 ℃, and adding the silicon dioxide and the concentrated solution in a ratio of 1: 2; sieving the dried intermediate, adding Concha Ostreae powder, and mixing.

In order to solve the fourth technical problem, the invention also discloses application of the traditional Chinese medicine composition and the preparation in preparing products for preventing and treating salpingitis of laying hens and application in preparing products for improving the laying performance of the laying hens.

Wherein, the product comprises but is not limited to veterinary drugs and feed additives.

Has the advantages that: compared with the prior art, the invention has the following advantages:

(1) the preparation process of the invention adopts fractional extraction, adopts dilute hydrochloric acid ethanol solution to extract leonurine, and extracts other effective components by water, thus improving the extraction yield of the effective components; the silicon dioxide adsorption drying process is adopted, so that the drying time is shortened, the drying temperature is reduced, and the product fluidity is improved; the oyster powder is not extracted, is directly crushed and compounded with extracted medicaments, plays the effects of warming the kidney, supporting yang, softening hardness and dissipating stagnation of the oyster, fully utilizes organic calcium and phosphorus in the oyster, supplements calcium and phosphorus for laying hens, and improves the quality of eggshells.

(2) The composition provided by the invention has the effects of clearing away heat and toxic materials, inducing diuresis with bland drugs and resisting inflammation, can be used for remarkably controlling salpingitis of laying hens in laying hen breeding and improving the quality of the laying hens, has a definite curative effect, and is suitable for antibiotic reduction in nonreactive breeding and livestock husbandry industries. Under the background that the use of antibiotics is limited and the antibiotics are controlled to be remained in animal products by the current national policy, the culture benefit can be improved, the egg quality is improved, and the method has huge market prospect and has obvious economic benefit and social benefit.

(3) The composition provided by the invention is a green and safe novel feed additive prepared from pure traditional Chinese medicines, and has the characteristics of safety, no side effect, quick response, no residue, obvious effect and the like.

Drawings

The foregoing and/or other advantages of the invention will become further apparent from the following detailed description of the invention when taken in conjunction with the accompanying drawings.

Fig. 1 shows the daily laying rate of the hens during the test period.

FIG. 2 shows the daily defective egg rate of each type of eggs, from top to bottom, in the test period, eggs with blood spots, eggs with sand shell, eggs with soft shell, eggs with broken shell and abnormal eggs.

Detailed Description

The experimental methods described in the following examples are all conventional methods unless otherwise specified; the reagents and materials are commercially available, unless otherwise specified.

Example 1

The formula of the components is as follows: weighing 20kg of motherwort, 20kg of polygonum capitatum, 20kg of chinaroot greenbrier, 20kg of common cnidium fruit, 20kg of plantain herb and 80kg of oyster according to parts by weight, and screening and cleaning.

The preparation process comprises the following steps:

(1) weighing the raw materials in parts by weight respectively; pulverizing the above materials except Concha Ostreae into coarse powder of about 2 mm; adding 0.05mol/L diluted hydrochloric acid 50% ethanol solution, soaking for 60 min, reflux extracting for 2 hr, with the mass ratio of solvent to raw materials being 6:1, filtering to obtain filtrate and residue, extracting the filtrate, recovering ethanol, and adjusting pH to 7.0 with sodium hydroxide;

(2) decocting the residue with water for 2 times, wherein the weight ratio of water to the raw materials is 6:1, and the decocting time is 2 hr and 1.5 hr respectively;

(3) mixing filtrates, and concentrating to density of 1.05-1.08g/cm³(ii) a The vacuum degree and the evaporation temperature of each effect in the three-effect concentrator are as follows: first effect is-0.04-0.07 MPa, 85 ℃; second effect-0.06-0.08 MPa, 75 ℃, third effect-0.07-0.10 MPa, 65 ℃;

(4) adding silicon dioxide into the concentrated solution, mixing, drying at 60 ℃, and adding the silicon dioxide and the concentrated solution in a ratio of 1: 2;

(5) sieving the dried intermediate, adding 60 mesh Concha Ostreae powder, and mixing to obtain 155kg of product.

Example 2

The formula of the components is as follows: weighing 20kg of motherwort, 30kg of polygonum capitatum, 25kg of chinaroot greenbrier, 28kg of common cnidium fruit, 21kg of plantain herb and 95kg of oyster according to parts by weight, and screening and cleaning.

The preparation process is the same as in example 1.

Example 3

The formula of the components is as follows: weighing 26kg of motherwort, 22kg of polygonum capitatum, 29kg of chinaroot greenbrier, 23kg of common cnidium fruit, 25kg of plantain herb and 104kg of oyster according to parts by weight, and screening and cleaning.

The preparation process is the same as in example 1.

Example 4: clinical validation

1. Test drugs the water-soluble traditional Chinese medicine compound extracted in example 1.

2. The experimental animals are provided by a certain mechanized chicken farm in Nanjing, 60000 laying hens are bred in the farm, the variety is Su poultry No. 1, the breeding mode is cage breeding, and 5-7 feathers are bred in each cage. 24000 feathers of laying hens which are 90 weeks old, healthy, similar in weight and subjected to forced moulting are selected.

3. The test grouping test adopts an animal sample self-control method, wherein a blank control group (non-administration stage) is adopted before the administration of the laying hens, and an experimental control group (administration stage and withdrawal stage) is adopted after the administration. The grouping is shown in table 2.

Table 2: test grouping

4. Test method

The egg laying rate and defective egg rate of the chicken group and the number of various defective eggs (blood spot eggs, broken eggs, soft shell eggs, sand shell eggs and malformed eggs) in the sample range are measured by observing for 1 day before the test, and the mental states of the excrement and the chicken group are observed. From day 2, the patient is administered according to the requirement, the relevant indexes are recorded every day, the administration of the liquid medicine is stopped after 7 days, the relevant indexes of each group are continuously recorded for 3 days, and the test is finished. The survey index is shown in Table 3.

Table 3: survey index

5. Test results

5.1 example 1 extraction of Water-soluble Chinese medicinal Compound for improving laying rate of laying hens in laying period

The results are shown in fig. 1, and the test results show that the product of example 1 can improve the laying rate of the laying hens to a certain extent in the administration stage and a certain period of time after the withdrawal, and the increase of the laying rate can reach 5.25 percent at the lowest and 15 percent at the highest. It is noted that on day 1 and day 2 of withdrawal, the laying rate was reduced due to a sudden drop in air temperature as the chicken house suffered from typhoons, but was still higher than the laying rate without the drug. It is generally accepted that the optimum temperature for layer production is 19-22 deg.C, and below this temperature range, cold irritation may occur. The low temperature has great harm to the production performance of the laying hens, and can cause the reduction of the laying rate and the increase of the pecking rate.

5.2 example 1 improvement of defective egg rate and defective egg quantity of laying hens by extracting water-soluble Chinese medicinal compound

The results are shown in fig. 2, and the experimental results show that the product of example 1 can significantly reduce the number of defective eggs and the ratio of defective eggs to the total number of eggs laid per day. The defective egg rate reaches 32.222 ‰ in the non-administration stage, gradually decreases and stabilizes in the rest 11 days with administration of medicine, and stabilizes at 7.037 ‰ -8.394 ‰ in the later stage of experiment. The product of example 1 has the greatest effect on the shell-broken eggs and the shell-sanded eggs, and can reduce the rate of the shell-broken eggs by about 10 per thousand and the shell-sanded eggs by 8 per thousand.

The comprehensive results are as follows: after the chicken flocks are used, the effect is fast, the laying rate is obviously increased, and the defective egg rate is obviously reduced. The product in the example 1 can improve the gastrointestinal tract function by resisting infection, resisting oxidation and enhancing immunity, and further regulate and control the reproductive hormone level, so that the production performance of the laying hens can be obviously improved. The product is pure traditional Chinese medicine, is safe and reliable to use, and can be widely applied to livestock and poultry breeding.

The invention provides a traditional Chinese medicine composition for preventing and treating salpingitis of laying hens, a preparation process and an application thought and a method thereof, and particularly provides a plurality of methods and ways for realizing the technical scheme. All the components not specified in the present embodiment can be realized by the prior art.

Claims

1. A traditional Chinese medicine composition for preventing and treating salpingitis of laying hens is characterized in that active ingredients of the traditional Chinese medicine composition comprise substances prepared according to the following formula; the formula comprises the following traditional Chinese medicine components: herba Leonuri, herba Polygoni Capitati, rhizoma Smilacis chinensis, fructus Cnidii, herba plantaginis, and Concha Ostreae.

2. The traditional Chinese medicine composition for preventing and treating salpingitis of laying hens as claimed in claim 1, wherein the components comprise, by mass, 20-30 parts of motherwort herb, 20-30 parts of polygonum capitatum, 20-30 parts of smilax china, 20-30 parts of common cnidium fruit, 20-30 parts of plantain herb and 80-120 parts of oyster.

3. The traditional Chinese medicine composition for preventing and treating salpingitis of laying hens as claimed in claim 1, wherein the components comprise, by mass, 20 parts of motherwort herb, 20 parts of polygonum capitatum, 20 parts of smilax china, 20 parts of common cnidium fruit, 20 parts of plantain herb and 80 parts of oyster.

4. The traditional Chinese medicine composition for preventing and treating salpingitis of laying hens as claimed in claim 1, wherein the components comprise 20g of motherwort herb, 20g of polygonum capitatum, 20g of smilax china, 20g of common cnidium fruit, 20g of plantain herb and 80g of oyster.

5. A preparation for preventing and treating salpingitis of laying hens, which is characterized by comprising the traditional Chinese medicine composition of any one of claims 1 to 3.

6. The method for preparing the traditional Chinese medicine composition of any one of claims 1 to 4, which is characterized by comprising the following steps:

s1: according to the formula, crushing the components except the oysters, performing reflux extraction by using an acidic alcohol solution, and filtering to obtain filtrate and dregs; recovering ethanol from the obtained filtrate, and adjusting the pH value to 6.5-7.5 for later use; decocting the residue with water, filtering, and mixing filtrates;

s3: and (8) mixing the dried product of S2 with oyster to obtain the product.

7. The method for preparing the Chinese medicinal composition according to claim 6, wherein in step S1, the acidic alcohol solution is a mixed solution of hydrochloric acid and ethanol.

8. The method for preparing a Chinese medicinal composition according to claim 7, wherein the concentration of the hydrochloric acid is 0.02-0.08 mol/L.

9. The use of the Chinese medicinal composition of any one of claims 1-4, or the formulation of claim 5, in the preparation of a product for preventing and treating salpingitis of laying hens.

10. Use of the Chinese medicinal composition of any one of claims 1-4, or the formulation of claim 5, in the preparation of a product for improving laying performance of laying hens.