CN113892486A - Egg laying repellent for female moths of mulberry moths, preparation method and application thereof - Google Patents
Egg laying repellent for female moths of mulberry moths, preparation method and application thereof Download PDFInfo
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- CN113892486A CN113892486A CN202111182554.4A CN202111182554A CN113892486A CN 113892486 A CN113892486 A CN 113892486A CN 202111182554 A CN202111182554 A CN 202111182554A CN 113892486 A CN113892486 A CN 113892486A
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- mulberry
- repellent
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses a repellent for egg laying of female moths of mulberry moths, which comprises benzaldehyde, acetic acid leaf alcohol ester, 1-hexadecene and 2, 6-diphenyl phenol. The invention can prevent the egg laying of the female moths of the mulberry borers, and correspondingly can remarkably reduce the egg laying amount of the female moths of the mulberry borers on the mulberry trees, thereby reducing the population quantity of the larvae of the mulberry borers, the harm to the mulberry trees and the control times of the traditional chemical pesticide.
Description
Technical Field
The invention relates to the technical field of repellents, in particular to a mulberry stem borer female moth oviposition repellent, a preparation method and application thereof.
Background
The mulberry stem borer (Diaphania pylosalis (Walker)) belongs to Lepidoptera and Pyralidae, and is one of the major pests of mulberry. Due to the characteristics of large reproduction quantity, short reproduction period, obvious generation overlapping, difficult prevention and control and the like, the silkworm mulberry field is easy to outbreak and cause disasters under proper conditions, and the continuous development of the silkworm mulberry industry is seriously influenced. At present, the traditional chemical prevention and control means is mainly used for preventing and controlling the pests in production, and the high prevention and control effect is obtained, and the defects of drug resistance of the pests, environmental pollution, pesticide residue, influence on safety of people and livestock, silkworm breeding poisoning and the like are also accompanied. Therefore, the research and development of a novel technology for efficiently preventing and treating the nuisanceless mulberry borers is urgently needed.
In recent years, based on the principles of insect chemoecology, new pest control means represented by an attracting technique have been increasingly emphasized and have been achieving good control effects. The actions of feeding, mating and laying eggs of insects almost do not leave the participation and control of the chemical sensing system. In long-term investigation research, it is found that the primary eclosion adult and the mated female adult can supplement nutrition and lay eggs on the most suitable host plant, volatile substances released by the host plant have strong guiding effect on host positioning of the adult, and the sensitive olfactory system of the adult plays an important role in olfactory communication such as host positioning, egg laying place selection and the like. Analyzing the addiction of pests to host plants and the composition of volatile matters of the pests, and screening and preparing an attractant by using the host plant volatile matters are important measures and ideas for controlling the pests; conversely, repellents made with odorous substances or volatile substances that are not palatable to pests are also gaining increasing attention in agricultural pest control. The repellent is utilized to interfere the egg laying behavior of the mulberry borers in the garden, the egg laying amount of the mulberry borers on the mulberry trees is reduced, and the method is a novel method for simply, conveniently and effectively preventing and treating the mulberry borers. At present, no efficient repellent specially aiming at egg laying of female multocida borers is found.
In the process of green prevention and control of mulberry trees, the harm degree of mulberry borers to hundreds of different varieties of mulberry trees in a mulberry tree variety garden is investigated to find that: in the same period, the damage degree of the mulberry borers to the mulberries of different varieties is different, and the egg laying condition of the mulberries borers on the young tips of the mulberries is different. Further carrying out dynamic headspace gas cycle method collection and GC-MS component identification on tender shoot volatile matters of the mulberry trees of 4 varieties with the heaviest harm to the mulberry borers and 4 varieties with the lightest harm to the mulberry borers. Further through multi-angle comparative analysis, the common components and the mutual components of the volatile matters are eliminated, and finally, benzaldehyde, acetic acid leaf alcohol ester, 1-hexadecene and 2, 6-diphenyl phenol are determined to be the target components of the repellent, wherein the target components are components which are not contained in the volatile matters of the tender tips of the severely damaged mulberry trees. Therefore, the research on the repellent effect of the 4 target components on egg laying of female moths after mating of the mulberry borers lays a foundation for developing the repellent for egg laying of the mulberry borers, and provides a new control technology and a product for green control of the mulberry borers.
Disclosure of Invention
The invention provides a repellent for egg laying of female moths of mulberry moths, a preparation method and application thereof, aiming at solving the defects in the prior art.
In order to solve the technical problems, the invention adopts the following technical scheme: a repellent for egg laying of female moth of mulberry borer comprises benzaldehyde, acetic acid leaf alcohol ester, 1-hexadecene and 2, 6-diphenyl phenol.
Preferably, the mass ratio of the benzaldehyde to the acetic acid phyllyl ester to the 1-hexadecene to the 2, 6-diphenyl phenol is (1-4): (3-7): (10-16): (2-3).
Preferably, the mass ratio of the benzaldehyde to the acetic acid phyllyl ester to the 1-hexadecene to the 2, 6-diphenyl phenol is 3: 5: 13: 3.
preferably, the repellent is obtained by dissolving the repellent in a heavy distilled n-hexane solution and stirring.
Preferably, the repellent is loaded on a slow release carrier to prepare a repellent lure core which is applied to repelling eggs of female moths of the mulberry borers.
Preferably, the slow release carrier is any one of filter paper, a cotton ball, a rubber plug and paraffin oil.
Preferably, the application time of the repellent is the period of the adult mulberry borers in the full growth period, and the periods are respectively from 4 months to 10 months per year in the middle and late days of each month;the application density is 5 per 667m2Distributed in mulberry field in Z shape; the application height is 30cm away from the mulberry crown; the application of attractant change/addition time interval is 5 d.
Compared with the prior art, the repellent provided by the invention can be used for repelling eggs of female moths of the mulberry borers, and correspondingly can remarkably reduce the egg laying amount of the female moths of the mulberry borers on mulberry trees, so that the population quantity of mulberry borers and the damage to the mulberry trees are reduced, the control frequency of traditional chemical pesticides is reduced, the pollution-free sustainable production of the mulberry trees is facilitated, a new way for controlling the damage of the mulberry borers in an environment-friendly and efficient manner is provided, the important significance is provided for promoting the high-quality development of silkworm silkworms, and the repellent and the application thereof have higher popularization and application values.
Detailed Description
The present invention will be described in detail with reference to examples.
Example 1
The invention provides a repellent for egg laying of female moths of mulberry moths, which comprises benzaldehyde, acetic acid leaf alcohol ester, 1-hexadecene and 2, 6-diphenyl phenol in a mass ratio of 1: 3: 10: 2.
example 2
The repellent comprises benzaldehyde, acetic acid leaf alcohol ester, 1-hexadecene and 2, 6-diphenyl phenol in a mass ratio of 3: 5: 13: 3.
example 3
The repellent comprises benzaldehyde, acetic acid leaf alcohol ester, 1-hexadecene and 2, 6-diphenyl phenol in a mass ratio of 4: 7: 16: 3.
preparation of examples 1 to 3: the solutions obtained in the embodiments 1 to 3 are respectively dissolved in heavy-distilled n-hexane to prepare solutions with the dosage of 100mg/ml, and the solutions are used as egg-laying repellents of the female moths of the mulberry borers.
The application time of the repellent is the period of the adult mulberry borers in the full growth period, and the period is 4-10 months each year in the middle and late days of each month; the application density is 5 per 667m2Distributed in mulberry field in Z shape; the application height is 30cm away from the mulberry crown; the application of attractant change/addition time interval is 5 d.
Comparing the examples 1-3 according to a repellent test, wherein the specific data are shown in a table 1;
repelling test: and (3) utilizing a wind tunnel device to perform behavior determination on the female mulberry borers, and verifying the repellent effect of the taste source on the mated female mulberry borers. Taking a mated female mulberry borer (2-3 days old) as a test object, taking 50 mu L of the prepared solution serving as the egg laying repellent of the female mulberry borer by using a micro sampler, dripping the solution on a filter paper strip of 3cm multiplied by 3cm to prepare a repellent core, and then placing the repellent core on a bracket (the bracket height is 35cm) between two vigorous mulberry pot plants (the average plant height is 50cm) to serve as a flavor source; placing the mulberry pot plant carrying the flavor source at the upwind end of a wind tunnel, and releasing female mulworms of the mulworms at the other end of the wind tunnel which is 1.5m away from the flavor source, wherein the size of the wind tunnel is 230cm multiplied by 85cm, and the wind speed is 0.2 m/s; observing each female moth for 5min, recording the time for the female moth to contact with the mulberry (taste source), and recording as no reaction if the female moth does not leave the release table within 5 min; repeat 3 times, each time test 20 female moths of mulberry borer.
TABLE 1
| Example 1 | Example 2 | Example 3 | |
| Number of female moths of mulberry borer | 60 | 60 | 60 |
| Number of approximation | 14 | 11 | 15 |
The garden investigation shows that different mulberry varieties are damaged by the mulberry borers to different degrees, the special components of the 4 mulberry twig volatile substances with low damage degrees are screened from the mulberry twig volatile substances of the 4 varieties with the highest damage degrees and the 4 varieties with the lowest damage degrees, the 4 substances are further tested to have a repellent effect on egg laying of the female mulberry borers, the combination effect of the 4 substances is the best, and the embodiment 2 is the optimal scheme as shown in the table 1.
Example 4
The preparation method of the repellent comprises the following steps: the repellent in the embodiment 2 is dissolved in heavy distilled n-hexane to prepare a solution with the dosage of 100mg/ml, and the solution is used as the egg laying repellent of the female moths of the mulberry borers.
Comparative example 1
Essentially identical to example 4, except that:
the preparation method of the repellent comprises the following steps: dissolving formaldehyde in heavy-distilled n-hexane to prepare a solution with the dosage of 100mg/ml, and using the solution as an oviposition repellent of the female moths of the mulberry borers.
Comparative example 2
Essentially identical to example 4, except that:
the preparation method of the repellent comprises the following steps: dissolving the leaf alcohol acetate in redistilled n-hexane to prepare a solution with the dosage of 100mg/ml, and using the solution as the egg-laying repellent of the female moths of the mulberry borers.
Comparative example 3
Essentially identical to example 4, except that:
the preparation method of the repellent comprises the following steps: dissolving 1-hexadecene in heavy-distilled n-hexane to prepare a solution with the dosage of 100mg/ml, and using the solution as the egg-laying repellent of the female moths of the mulberry borers.
Comparative example 4
Essentially identical to example 4, except that:
the preparation method of the repellent comprises the following steps: 2, 6-diphenyl phenol is dissolved in heavy-distilled n-hexane to prepare a solution with the dosage of 100mg/ml, and the solution is used as the egg laying repellent of the female moths of the mulberry borers.
Comparative example 5
Essentially identical to example 4, except that:
the preparation method of the repellent comprises the following steps: benzaldehyde and 2, 6-diphenylphenol were mixed in the following ratio of 3: 3, dissolving the mixture in heavy-distilled n-hexane to prepare a solution with the dosage of 100mg/ml, and using the solution as the egg laying repellent of the female moths of the mulberry borers.
Comparative example 6
Essentially identical to example 4, except that:
the preparation method of the repellent comprises the following steps: mixing folic alcohol acetate and 1-hexadecene according to the weight ratio of 5: 13, dissolving in heavy-distilled n-hexane to prepare a solution with the dosage of 100mg/ml, and using the solution as the egg-laying repellent of the female moths of the mulberry borers.
Comparative example 7
Essentially identical to example 4, except that:
the preparation method of the repellent comprises the following steps: benzaldehyde, folic alcohol acetate and 1-hexadecene are mixed according to the weight ratio of 3: 5: 13, dissolving in heavy-distilled n-hexane to prepare a solution with the dosage of 100mg/ml, and using the solution as the egg-laying repellent of the female moths of the mulberry borers.
Comparative example 8
Essentially identical to example 4, except that:
the preparation method of the repellent comprises the following steps: benzaldehyde, folic alcohol acetate and 2, 6-diphenyl phenol were mixed according to a 3: 5: 3, dissolving the mixture in heavy-distilled n-hexane to prepare a solution with the dosage of 100mg/ml, and using the solution as the egg laying repellent of the female moths of the mulberry borers.
Comparative example 9
Essentially identical to example 4, except that:
the preparation method of the repellent comprises the following steps: the repellent is heavy-distilled n-hexane which is prepared into a solution with the dosage of 100mg/ml as the egg laying repellent of the female moths of the mulberry borers.
Comparing the example 4 and the comparative examples 1-9 according to a repellent test, wherein the specific data are shown in a table 2;
repelling test: and (3) utilizing a wind tunnel device to perform behavior determination on the female mulberry borers, and verifying the repellent effect of the taste source on the mated female mulberry borers. Taking a mated female mulberry borer (2-3 days old) as a test object, taking 50 mu L of the prepared solution serving as the egg laying repellent of the female mulberry borer by using a micro sampler, dripping the solution on a filter paper strip of 3cm multiplied by 3cm to prepare a repellent core, and then placing the repellent core on a bracket (the bracket height is 35cm) between two vigorous mulberry pot plants (the average plant height is 50cm) to serve as a flavor source; placing the mulberry pot plant carrying the flavor source at the upwind end of a wind tunnel, and releasing female mulworms of the mulworms at the other end of the wind tunnel which is 1.5m away from the flavor source, wherein the size of the wind tunnel is 230cm multiplied by 85cm, and the wind speed is 0.2 m/s; observing each female moth for 5min, recording the time for the female moth to contact with the mulberry (taste source), and recording as no reaction if the female moth does not leave the release table within 5 min; repeat 3 times, each time test 20 female moths of mulberry borer.
TABLE 2
Table 2 is a data showing the effect of each repellent shown in example 4 and comparative examples 1 to 9 of the present invention on the repellency of the female moth of the mulberry borer. As can be seen from the table, compared with the control example 9, the directional behavior of the mulberry with the female mulberry borer is influenced by the repellents with different degrees, wherein the repellent with the 4 efficacy components in the example 4 has a repellent effect which is obviously stronger than that of the single products and the compositions in the control examples 1 to 8. Therefore, the results clearly show that the egg laying repellent for the female mulberry borer and the application thereof provided by the invention can be used for repelling the female mulberry borer and correspondingly achieve the effect of repelling the egg laying of the female mulberry borer, so that the population quantity and harm of the mulberry borer larvae are reduced, a new way is provided for environment-friendly and efficient prevention and control of mulberry borer harm to mulberry trees, the repellent has important significance for promoting high-quality development of silkworm mulberry, and the repellent and the application have wide economic, social and ecological benefits.
Those skilled in the art can select and design flexibly according to the needs, and is not limited thereto.
The above additional technical features can be freely combined and used in superposition by those skilled in the art without conflict.
The above examples only show some embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the patent and protection scope of the present invention should be subject to the appended claims.
Claims (7)
1. A repellent for egg laying of female moths of mulberry moths is characterized by comprising benzaldehyde, acetic acid leaf alcohol ester, 1-hexadecene and 2, 6-diphenyl phenol.
2. The egg laying repellent for the female moths of the mulberry borers, according to claim 1, is characterized in that the mass ratio of benzaldehyde, acetic acid leaf alcohol ester, 1-hexadecene and 2, 6-diphenyl phenol is (1-4): (3-7): (10-16): (2-3).
3. The egg laying repellent for the female moths of the mulberry borers, according to claim 1, is characterized in that the mass ratio of benzaldehyde, folic alcohol acetate, 1-hexadecene and 2, 6-diphenyl phenol is 3: 5: 13: 3.
4. the method for preparing the repellent according to the claims 1 to 3, wherein the repellent is obtained by dissolving the repellent in a redistilled n-hexane solution and stirring.
5. The application of the repellent according to claims 1 to 4, wherein the repellent is loaded on a slow release carrier to prepare a repellent lure core which is applied to repelling eggs of female moths of the mulberry borers.
6. The use of a repellent according to claim 6, wherein the slow release carrier is any one of filter paper, absorbent cotton balls, rubber stoppers and paraffin oil.
7. Use as claimed in claim 6, for a repellent for application at a time when the adult mulberry borer is exuberantThe growth period is respectively from 4 months to 10 months per year in the middle and last days of each month; the application density is 5 per 667m2Distributed in mulberry field in Z shape; the application height is 30cm away from the mulberry crown; the application of attractant change/addition time interval is 5 d.
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| CN202111182554.4A CN113892486B (en) | 2021-10-11 | 2021-10-11 | Egg laying repellent for female moths of mulberry moths, preparation method and application thereof |
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| CN202111182554.4A CN113892486B (en) | 2021-10-11 | 2021-10-11 | Egg laying repellent for female moths of mulberry moths, preparation method and application thereof |
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| CN113892486B CN113892486B (en) | 2022-07-29 |
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Cited By (1)
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| CN115968882A (en) * | 2023-02-02 | 2023-04-18 | 四川省新兰月生物科技有限公司 | Pharmaceutical composition for promoting citrus color change and preparation method thereof |
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| CN113142202A (en) * | 2021-04-23 | 2021-07-23 | 贵州师范大学 | Method for preventing and controlling noctuid pests in field crops |
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2021
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| CN111096323A (en) * | 2019-12-27 | 2020-05-05 | 四川省金桑庄园农业发展股份有限公司 | Mulberry borer attractant and application thereof |
| CN113142202A (en) * | 2021-04-23 | 2021-07-23 | 贵州师范大学 | Method for preventing and controlling noctuid pests in field crops |
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