CN113848335A - Automatic processing system for liquid-based cytology and human papilloma virus combined screening sample - Google Patents
Automatic processing system for liquid-based cytology and human papilloma virus combined screening sample Download PDFInfo
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- CN113848335A CN113848335A CN202111138198.6A CN202111138198A CN113848335A CN 113848335 A CN113848335 A CN 113848335A CN 202111138198 A CN202111138198 A CN 202111138198A CN 113848335 A CN113848335 A CN 113848335A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00722—Communications; Identification
- G01N35/00732—Identification of carriers, materials or components in automatic analysers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
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Abstract
The invention provides an automatic processing system for jointly screening samples by liquid-based cytology and human papilloma virus, which comprises a sampling brush conveyer belt, wherein the sampling brush conveyer belt is used for conveying a sampling brush, and a handle shearing mechanism is arranged on a motion path of the sampling brush conveyer belt and is used for shearing a sampling brush rod of the sampling brush; the elution bottle conveying belt is also arranged and is used for placing the elution bottle below the handle shearing mechanism to receive the sampling brush head and elute the collected sample; the device is also provided with a liquid separating bottle cap which is provided with a liquid separating joint for separating the eluent in the elution bottle into a preservation test tube and a cracking test tube; still be equipped with the arm, the arm is used for snatching the liquid separating bottle lid and covers on the elution bottle. The invention can automatically finish the collection of the cell sample by the sampling brush, and can respectively inject the cell sample into the preservation test tube and the lysis test tube without mutual interference, thereby greatly improving the efficiency of sample collection.
Description
Technical Field
The invention relates to the field of cervical cancer joint screening, in particular to an automatic processing system for joint screening samples of liquid-based cytology and human papilloma virus.
Background
In recent years, cervical cancer is a trend of youthfulness and is a common gynecological malignant tumor seriously threatening the health of women. The etiology of cervical cancer is clear, and high-risk Human Papilloma Virus (HPV) infection is the primary condition for the occurrence of cervical cancer. The cervical cancer can be detected, diagnosed and treated early, so that early screening is an important measure for preventing and treating the cervical cancer. With the development of cervical cancer screening technology in recent years, liquid-based thin-layer cytology screening (TCT) is taken as an important diagnosis technology for early screening cervical cancer lesions, plays a role of a gold standard in cervical cancer pre-lesion diagnosis, and HPV-DNA (human papillomavirus) -proves the existence and the load of pathogens in advance from the perspective of molecular genetics, and can effectively reduce false negative of cytological examination, so that the combined detection of TCT and cervical HPV-DNA can play an increasingly important clinical value in large-scale cervical cancer screening.
The liquid-based thin-layer cell technology TCT is used for screening early cervical lesions, can obviously improve the detection rate of abnormal cells, has high sensitivity, makes up the defects of the traditional Papanicolaou classification, realizes the basic unification of cytopathology and histopathology terms, and can provide richer and more detailed diagnosis information for clinicians through the image-text report issued by computer-aided reading. The liquid-based thin-layer cell technology can prepare a high-quality cervical cell smear, so that the morphological structure of cells is clearer, the detection rate of cervical cancer is greatly improved, and the cytological diagnosis level is gradually improved. Meanwhile, partial precancerous lesions and microbial infections such as fungi, trichomonas, viruses, chlamydia and the like can be found. TBS reporting can also provide an assessment of specimen quality and recommendations for diagnosis. But the united screening has the non-uniform problem of specimen preservation after sampling at present. The sampling step of cervical cancer screening is to collect the exfoliated cells of the external cervical orifice and the cervical canal by using a broom-shaped cervical brush sampler, as recorded by a cervical cancer sampling device-CN 212118190U. Then eluted in a preservative or lysis solution. Wherein, the TCT detection sample needs to be fixed and preserved by a preserving fluid without destroying the original structure or components of cells, aiming at leading the substances in the cells to be as close as possible to the morphological structure and position of the cells in the living state; the key to preservation and pretreatment of HPV-DNA detection is the need to disrupt cell morphology in the lysate, enzymatically hydrolyze, denature or hydrolyze cell membranes, release chromosomes, enzymatically hydrolyze nucleic acid-bound histones. Due to different functions of the preservation solution or the lysate, the preservation solution or the lysate generally needs to be collected twice and eluted in the preservation solution or the lysate respectively, so that the process is very complicated, and the problem of inconsistent samples is easily caused by collecting twice, so that a better solution scheme is not seen at present.
In the prior art, a sample report for developing cervical cancer screening by an artificial intelligence technology can be rapidly obtained, for example, an artificial intelligence cloud diagnosis platform is recorded in CN110797097A, which can greatly improve the efficiency and accuracy of sample identification, but the bottleneck of cervical cancer screening lies in that the efficiency of sample collection and pretreatment is low, which hinders further improvement of cervical cancer screening efficiency.
Disclosure of Invention
The invention aims to solve the technical problem of providing an automatic sample processing system for liquid-based cytology and human papilloma virus combined screening, which can automatically acquire and obtain a unified sample of TCT and cervical HPV-DNA, improve the detection accuracy and has high acquisition efficiency.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows: an automatic processing system for screening samples by combining liquid-based cytology and human papilloma virus comprises a sampling brush conveyer belt, wherein the sampling brush conveyer belt is used for conveying a sampling brush, and a movement path of the sampling brush conveyer belt is provided with a shearing handle mechanism for shearing a sampling brush rod of the sampling brush;
the elution bottle conveying belt is also arranged and is used for placing the elution bottle below the handle shearing mechanism to receive the sampling brush head and elute the collected sample;
the device is also provided with a liquid separating bottle cap which is provided with a liquid separating joint for separating the eluent in the elution bottle into a preservation test tube and a cracking test tube;
still be equipped with the arm, the arm is used for snatching the liquid separating bottle lid and covers on the elution bottle.
In the preferred scheme, the sample brush conveyer belt is the reciprocal conveyer belt of circulation, and the sample brush conveyer belt is equipped with vertical face, is equipped with a plurality of peg on the vertical face, and the sample brush is hung on one of them peg.
In the preferred scheme, the structure of the shearing handle mechanism is that a rod feeding piece is arranged on a shearing handle bracket, a rod feeding opening is arranged on the rod feeding piece, the rod feeding opening is positioned on the motion path of the sampling brush, a shearing handle cutter is arranged at the position of the rod feeding opening, the shearing handle cutter is connected with the rod feeding piece in a relatively rotating mode through a shaft, the outer edge of the shearing handle cutter is provided with an arc, teeth are arranged on the arc, a shearing handle half gear is meshed with the teeth on the outer edge of the shearing handle cutter, and the shearing handle half gear is connected with a motor;
and a return spring for returning the scissors handle knife is also arranged.
In the preferred scheme, the conveying belt of the elution bottle adopts a chain plate type conveying belt, and the surface of a single chain plate is provided with a bottle seat for limiting the elution bottle;
the conveying belt of the elution bottle is driven by a stepping motor.
In a preferable scheme, a vibrator is arranged in the bottle seat, and the vibrator enables liquid in the elution bottle to vibrate.
In the preferred scheme, a bottle supply device is further arranged, the bottle supply device supplies elution bottles by adopting a rotary friction mechanism, and an eluent liquid supply device is arranged on the path of the bottle supply device and is used for supplying eluent to the elution bottles;
the bottle seat is provided with a notch corresponding to the bottle supplying device.
In the preferred scheme, a bottle cap supply chain is further arranged, chains are arranged on two sides of the bottle cap supply chain, a chain rod is arranged in the middle of the bottle cap supply chain, and a liquid separating bottle cap is placed between the two chain rods.
In the preferred scheme, the test tube;
the test tube conveyer belt is equipped with gland device from the below process of elution bottle conveyer belt, in the crisscross position of elution bottle conveyer belt and test tube conveyer belt for inject into respectively the eluant of in the elution bottle and preserve test tube and schizolysis test tube.
In a preferred scheme, the structure of the liquid separating bottle cap is as follows: a Y-shaped liquid separation joint is arranged on the cover body, one end of the liquid separation joint is connected with a liquid inlet pipe, the liquid inlet pipe is positioned in the elution bottle, the other end of the liquid separation joint is respectively connected with two liquid separation pipes, and the liquid separation pipes are positioned outside the elution bottle;
the cover body is also provided with a pressing column which is connected with the cover body in a sealing and sliding way;
the gland device is provided with a gland cylinder for pressing the compression leg down below the cover body.
In a preferable scheme, a first laser scanner is arranged on a traveling path of a sampling brush conveying belt;
a second laser scanner is arranged on a bottle supply path of the bottle supply device;
a third laser scanner is arranged on the conveying path of the test tube conveying belt;
the first laser scanner is used for scanning the identification code of the sampling brush;
the second laser scanner is used for scanning the identification code of the elution bottle;
the third laser scanner is used for scanning the identification code of the test tube rack;
the corresponding identification codes are coded into a group, and the identification codes of the preservation test tubes and the cracking test tubes in the test tube rack are also coded into a group with the identification codes of the test tube rack.
The automatic processing system for the liquid-based cytology and human papilloma virus combined screening sample can automatically finish the collection of the sampling brush cell sample, and can be respectively injected into a preservation test tube and a lysis test tube without mutual interference, so that the sample collection efficiency is greatly improved, and the bottleneck influencing cervical cancer screening is broken. The system has a simpler structure, a small number of positions needing accurate positioning among all actions, fewer auxiliary mechanisms and higher reliability, and the sampling brushes, the elution bottles, the storage test tubes and the cracking test tubes are in one-to-one correspondence in a mode of scanning identification codes by the arranged multiple laser scanners.
Drawings
The invention is further illustrated by the following examples in conjunction with the accompanying drawings:
fig. 1 is a schematic top view of the present invention.
FIG. 2 is a schematic view of a partially developed structure of a conveyor belt and a shear handle mechanism of the sampling brush of the present invention.
Fig. 3 is a schematic top view of the handle shearing mechanism of the present invention.
FIG. 4 is a schematic view showing a partial structure of a transfer belt for an elution bottle of the present invention.
Fig. 5 is a schematic view of the operation state of the capping device according to the present invention.
Fig. 6 is a schematic view of the working state of the liquid separation joint in the invention.
Fig. 7 is a schematic cross-sectional view of the separating bottle cap of the invention when being transported on a bottle cap chain.
In the figure: the elution bottle 1, the bottle feeding device 2, the bottle feeding cap chain 3, the chain 31, the chain rod 32, the liquid separating cap 4, the liquid separating joint 41, the rotating shaft 411, the front plate 412, the first liquid separating plate 413, the second liquid separating plate 414, the liquid inlet pipe 42, the liquid separating pipe 43, the cover 44, the compression column 45, the sampling brush conveyer belt 5, the hanging rod 51, the mechanical arm 6, the test tube rack 7, the test tube conveyer belt 8, the first pump 9, the second pump 10, the first liquid supply tank 11, the second liquid supply tank 12, the capping device 13, the capping bracket 131, the capping cylinder 132, the third laser scanner 14, the second laser scanner 15, the first laser scanner 16, the sampling brush inlet 17, the negative pressure plugging device 18, the handle taking slope 19, the elution bottle conveyer belt 20, the bottle base 201, the vibrator 202, the elution bottle collecting tank 21, the handle shearing mechanism 22, the handle shearing bracket 221, the handle shearing half gear 222, the handle shearing cutter 223, the return spring 224, the rod inlet opening 225, the rod feeding plate 226, the box 23 is collected to the sample brush, and sample brush 24, identification code 241, sample brush holder 242, sample brush-holder 243, sample brush head 244, save test tube 25, schizolysis test tube 26, eluent liquid supply unit 27, casing 28.
Detailed Description
Example 1:
as shown in figure 1, the automatic sample processing system for liquid-based cytology and human papilloma virus combined screening comprises a sampling brush conveyer belt 5, preferably, as shown in figure 2, the sampling brush conveyer belt 5 is a reciprocating conveyer belt, the sampling brush conveyer belt 5 is provided with a vertical surface, a plurality of hanging rods 51 are arranged on the vertical surface, and a sampling brush 24 is hung on one of the hanging rods 51. Optionally, a fixed rod is provided on the vertical surface of the sampling brush conveyor belt 5, and a hook is provided on the sampling brush holder 242 of the sampling brush 24, and the hook is hung on the rod. Alternatively, a fork-shaped hook is arranged on the vertical surface of the sampling brush conveyer belt 5, and the sampling brush holder 242 is hung on the hook.
As shown in fig. 2, a cutting mechanism 22 for cutting off a sampling brush rod 243 of the sampling brush 24 is provided on the movement path of the sampling brush conveyer belt 5; the sampling brush head 244 falls into the lower elution bottle 1, and the elution liquid is injected into the elution bottle 1, wherein the elution liquid comprises the components of sodium chloride and is 0.420 percent by mass; concentrated hydrochloric acid, the volume percent is 0.062%; the balance of water; for eluting the sample from the sampling brush head 244 and separating the cellular sample from impurities. The scheme can improve the elution efficiency and simulate the environment in a human body.
As shown in fig. 1 and 2, a handle slope 19 is further provided at a position downstream of the handle shearing mechanism 22 in the moving path of the sampling brush belt 5, and when the sampling brush handle 242 and the sampling brush rod 243, from which the sampling brush head 244 is sheared, run to the position of the handle slope 19, the sampling brush handle 242 and the sampling brush rod 243 are separated from the vertical surface of the sampling brush belt 5.
As shown in fig. 1, an elution bottle conveyer belt 20 is further provided for placing the elution bottle 1 under the handle shearing mechanism 22 to receive the sampling brush head 244 and elute the collected sample; in a preferred scheme, the elution bottle conveying belt 20 is a chain plate type conveying belt, a bottle seat 201 is arranged on the surface of a single chain plate, and preferably, the bottle seat 201 is a groove with a single side opening and is used for limiting the elution bottle 1;
the elution vial conveyor belt 20 is driven by a stepper motor. A photoelectric sensor is disposed at one side of the elution bottle conveyer belt 20 to detect whether there is an elution bottle 1 in the current bottle seat 201 and whether the elution bottle 1 is located at a preset position.
As shown in fig. 1, a separating bottle cap 4 is further provided, and a separating joint 41 is provided on the separating bottle cap 4 for separating the eluent in the elution bottle 1 to the preservation test tube 25 and the lysis test tube 26; in a preferred scheme, the separating bottle caps 4 are continuously conveyed by the bottle cap supply chain 3. The two sides of the bottle cap chain 3 are provided with chains 31, the middle of the bottle cap chain is provided with chain rods 32, the liquid separating bottle caps 4 are placed between the two chain rods 32, and the cover bodies 44 of the liquid separating bottle caps 4 realize limiting.
Still be equipped with arm 6, arm 6 is used for snatching separating bottle lid 4 and covers on elution bottle 1. The robot arm 6 in this example is a six-axis robot arm.
The preferable scheme is as shown in fig. 1-3, the handle shearing mechanism 22 is characterized in that a rod feeding piece 226 is arranged on the handle shearing support 221, a rod feeding opening 225 is arranged on the rod feeding piece 226, the rod feeding opening 225 is positioned on the motion path of the sampling brush 24, a handle shearing knife 223 is arranged at the position of the rod feeding opening 225, the handle shearing knife 223 is connected with the rod feeding piece 226 in a relatively rotating mode through a shaft, a knife edge is arranged on the handle shearing knife 223, the rotating position of the knife edge part and the rod feeding opening 225 form a shearing structure, an arc is arranged at the outer edge of one end, close to the rotating shaft, of the handle shearing knife 223, teeth are arranged on the arc, a handle shearing half gear 222 is in meshing connection with the teeth at the outer edge of the handle shearing knife 223, and the handle half gear 222 is connected with a motor; the motor is provided with a speed reducer. A photoelectric sensor is arranged on one side of the sampling brush conveying belt 5, when the photoelectric sensor detects that a sampling brush rod 243 of the sampling brush 24 enters the rod inlet opening 225, the motor drives the scissor handle half gear 222 to rotate, the scissor handle half gear 222 drives the scissor handle knife 223 to rotate to cut the sampling brush rod 243, and the sampling brush head 244 falls into the lower elution bottle 1 for elution. A return spring 224 for returning the shank cutter 223 is provided on the rotary shaft of the shank cutter 223. When the half-gear 222 is rotated to disengage the knife 223, the knife 223 is reset by the reset spring 224 and the motor stops.
In a preferred embodiment, as shown in FIG. 4, a vibrator 202 is provided in the bottle holder 201, and the vibrator 202 vibrates the liquid in the elution bottle 1. Preferably, the vibrator 202 is an ultrasonic vibrator, and the vibrator 202 causes cavitation bubbles to be generated in the eluent in the elution bottle 1, thereby greatly improving the elution effect.
The preferable scheme is as shown in fig. 1, a bottle supplying device 2 is further provided, the bottle supplying device 2 supplies the elution bottles 1 by adopting a rotary friction mechanism, the elution bottles 1 are extruded into a bottle seat 201 of an elution bottle conveying belt 20 from a channel one by one through a rotary chassis, and an eluent liquid supplying device 27 is arranged on a bottle supplying path and used for supplying eluent to the elution bottles 1; the eluent liquid supply device 27 comprises a peristaltic pump or a diaphragm pump and is also provided with a liquid supply box, and the peristaltic pump or the diaphragm pump pumps the eluent in the liquid supply box to inject into the passing elution bottle 1.
A notch corresponding to the bottle supplying device 2 is provided on the bottle seat 201 to facilitate the entry of the elution bottle 1.
According to a preferable scheme, as shown in fig. 1, a test tube conveyer belt 8 is further arranged, the test tube conveyer belt 8 is a crawler-type conveyer belt, the test tube conveyer belt 8 is used for conveying test tube racks 7, the test tube racks 7 are of a square structure so as to be arranged orderly, a storage test tube 25 and a lysis test tube 26 which are parallel to each other are arranged in each test tube rack 7, and a first pump 9 and a second pump 10 which are used for respectively injecting liquid into the storage test tube 25 and the lysis test tube 26 are arranged above the test tube conveyer belt 8; a first liquid supply box 11 for respectively supplying preservation liquid and a second liquid supply box 12 for preserving lysate are also arranged, and the first pump 9 and the second pump 10 adopt peristaltic pumps or diaphragm pumps.
The component of the preserving fluid in the preserving test tube 25 is N-acetyl-3-mercapto alanine, the mass percentage is 0.11%; absolute alcohol, the volume percentage is 49.38%; 0.173 percent of disodium hydrogen phosphate by mass; 0.012 percent of sodium dihydrogen phosphate; glacial acetic acid, volume percent is 0.59%; the balance of water; the amount of water required 1/2 is calculated from the amount of eluent water injected.
The composition of the lysate in lysis tube 26 is: sodium dodecyl sulfate, volume percent 1-5%; 0.5 to 2 percent of polyethylene glycol octyl phenyl ether; 100-300mmol/L of 4-hydroxyethyl piperazine ethanesulfonic acid; sodium chloride, 7.6-11.6% by volume; 50-150mmol/L of trihydroxymethyl aminomethane hydrochloride; ethylene diamine tetraacetic acid, 0.5-3 mmol/L; 1-3% of lauryl alcohol polyoxyethylene ether sulfate sodium salt by volume percentage; the balance being water. The amount of water required 1/2 is calculated from the amount of eluent water injected.
As shown in fig. 5, the test tube conveyer belt 8 passes under the elution bottle conveyer belt 20, and a capping device 13 is provided at a position where the elution bottle conveyer belt 20 and the test tube conveyer belt 8 are crossed, for injecting the eluents in the elution bottle 1 into the preservation test tube 25 and the lysis test tube 26, respectively.
In a preferred scheme, as shown in fig. 7, the structure of the separating bottle cap 4 is as follows: a Y-shaped liquid separating joint 41 is arranged on the cover body 44, one end of the liquid separating joint 41 is connected with a liquid inlet pipe 42, the liquid inlet pipe 42 is positioned in the elution bottle 1, the other end of the liquid separating joint 41 is respectively connected with two liquid separating pipes 43, and the liquid separating pipes 43 are positioned outside the elution bottle 1; further preferred is a scheme as shown in fig. 6, a passive liquid-separating structure is further arranged at the liquid-separating joint 41, the position of the Y-shaped structure of the liquid-separating joint 41 is rectangular in cross section, a vertical rotating shaft 411 is arranged at the middle position, one side of the rotating shaft 411 is connected with a front piece 412, the other side of the rotating shaft is connected with a first liquid-separating piece 413 and a second liquid-separating piece 414, when liquid enters the front piece 412, the front piece 412 is made to swing randomly, so that the liquid of the eluent is randomly distributed between the two branch pipes, and the random quantity is approximately the same in a time period.
The cover body 44 is also provided with a pressure column 45, and the pressure column 45 is connected with the cover body 44 in a sealing and sliding way; the pressure column 45 has a substantially cylindrical structure, and when the lid 44 is closed and sealed in the elution bottle 1, the pressure column 45 is pressed down by the capping device 13, so that the positive pressure causes the eluent to pass through the liquid inlet tube 42, the liquid separating joint 41 and the two liquid separating tubes 43 from the elution bottle 1 and to be injected into the preservation test tube 25 and the lysis test tube 26, respectively, even if the positive pressure is formed in the elution bottle 1, thereby completing the liquid preparation operation. The adoption of the scheme has the advantage that the liquid separation quality with higher precision can be obtained. And compared with the conventional liquid transfer operation, the efficiency is greatly improved, and the cost is greatly reduced.
The capping device 13 is provided with a capping cylinder 132 for pressing the plunger 45 down below the cap body 44.
In a preferred embodiment, as shown in fig. 1, a first laser scanner 16 is provided on the traveling path of the sampling brush transporting belt 5;
a second laser scanner 15 is provided on a bottle supply path of the bottle supply device 2;
a third laser scanner 14 is arranged on the conveying path of the test tube conveying belt 8;
the first laser scanner 16 is used to scan the identification code of the sample brush 24; the identification code may be a bar code, a two-dimensional code, or other form of pattern that is easily digitized.
The second laser scanner 15 is used for scanning the identification code of the elution bottle 1;
the third laser scanner 14 is used for scanning the identification code of the test tube rack 7;
the corresponding identification codes are coded into a group, and the corresponding identification codes refer to that the sampling brush head 244 of the sampling brush 24 falls into the corresponding elution bottle 1, and the eluent in the elution bottle 1 is injected into the corresponding preservation test tube 25 and the lysis test tube 26. The identification codes of the preservation tubes 25 and the lysis tubes 26 in the tube rack 7 are also grouped with the identification code of the tube rack 7. According to the scheme, only the identification codes of the test tube rack 7 need to be scanned, and the identification codes of the storage test tubes 25 and the splitting test tubes 26 can be grouped.
Example 2:
in use, as shown in fig. 1, after a doctor operates the sampling brush 24 to sample a cervix position, the sampling brush 24 is hung on the hanging rod 51 of the sampling brush conveyer belt 5 from the position of the sampling brush inlet 17, the first laser scanner 16 scans the identification code 241 on the sampling brush handle 242 to form a user code group x, the sampling brush conveyer belt 5 rotates to the position of the handle shearing mechanism 22, at this time, the elution bottle 1 is supplied to the bottle seat 201 on the elution bottle conveyer belt 20 by the bottle supply device 2, the elution bottle 1 is injected with a proper amount of eluent by the eluent supply device 27, and the identification code of the elution bottle 1 is scanned by the second laser scanner 15 and then is included in the user code group x. The motor of the handle shearing mechanism 22 drives the handle shearing half gear 222 to rotate, the handle shearing half gear 222 drives the handle shearing knife 223 to shear the sampling brush rod 243 of the current sampling brush 24, and the sampling brush head 244 falls into the elution bottle 1, as shown in fig. 2. The sampling brush transport belt 5 continues to rotate and handles the other sampling brushes 24. The current sampling brush falls off the sampling brush conveyor belt 5 under the action of the grip ramp 19 and falls into the sampling brush collection box 23. The elution vial conveyor belt 20 is stepped. The elution bottle 1 is brought to the next station, where there is no shielding above, and the vibrator 202 in the bottle seat 201 vibrates to elute the sample on the sampling brush head 244. The mechanical arm 6 acts to grab the liquid separating bottle cap 4 on the bottle cap chain 3 to cover the current elution bottle 1. The elution bottle conveyer belt 20 drives the elution bottle 1 to enter the lower part of the capping device 13 step by step. The test tube conveyer belt 8 is used for conveying the test tube rack 7 to the position below the elution bottle 1 at the current station step by step. During the transfer process, the first pump 9 and the second pump 10 are used to inject the storage solution into the storage tube 25 and the lysis solution into the lysis tube 26, respectively. The capping cylinder 132 of the capping device 13 is actuated to press down the pressure column 45, and the eluent is introduced into the preservation test tube 25 and the lysis test tube 26 from the liquid inlet tube 42, the liquid separation joint 41 and the liquid separation tube 43 under pressure. When the test tube rack 7 passes through the third laser scanner 14, the third laser scanner 14 scans the identification code of the test tube rack 7, and also incorporates the identification codes of the storage test tubes 25 and the lysis test tubes 26 into the user code group x, thereby completing the binding of the related data. The test tube conveyer belt 8 continues to run, and the bottle stopper is covered by vacuumizing at the position of the negative pressure stopper 18. Sending to the next step for processing. The elution bottle conveyer belt 20 continues to run, and the elution bottle 1 falls into the elution bottle collecting box 21. By the system, automatic processing of liquid-based cytology and human papilloma virus combined screening samples is realized, processing efficiency is greatly improved, and errors caused by data inconsistency are avoided. And the structure is simple, and the realization cost is low.
The above-described embodiments are merely preferred embodiments of the present invention, and should not be construed as limiting the present invention, and features in the embodiments and examples in the present application may be arbitrarily combined with each other without conflict. The protection scope of the present invention is defined by the claims, and includes equivalents of technical features of the claims. I.e., equivalent alterations and modifications within the scope hereof, are also intended to be within the scope of the invention.
Claims (10)
1. An automatic processing system for screening samples by combining liquid-based cytology and human papilloma virus is characterized in that: the sampling brush conveying belt (5) is used for conveying a sampling brush (24), a handle shearing mechanism (22) and a sampling brush rod (243) for shearing the sampling brush (24) are arranged on a movement path of the sampling brush conveying belt (5);
the elution bottle conveying belt (20) is also arranged and is used for placing the elution bottle (1) below the handle shearing mechanism (22) to receive the sampling brush head (244) and elute the collected sample;
the device is also provided with a liquid separating bottle cap (4), wherein a liquid separating joint (41) is arranged on the liquid separating bottle cap (4) and is used for separating the eluent in the elution bottle (1) into a preservation test tube (25) and a cracking test tube (26);
the washing machine is also provided with a mechanical arm (6), and the mechanical arm (6) is used for grabbing the liquid separating bottle cap (4) to cover the washing bottle (1).
2. The system of claim 1, wherein the automated system comprises: the sampling brush conveying belt (5) is a circulating reciprocating conveying belt, the sampling brush conveying belt (5) is provided with a vertical surface, a plurality of hanging rods (51) are arranged on the vertical surface, and the sampling brush (24) is hung on one of the hanging rods (51).
3. The system of claim 1, wherein the automated system comprises: the structure of the handle shearing mechanism (22) is that a rod feeding sheet (226) is arranged on a handle shearing support (221), a rod feeding opening (225) is arranged on the rod feeding sheet (226), the rod feeding opening (225) is positioned on the motion path of the sampling brush (24), a handle shearing knife (223) is arranged at the position of the rod feeding opening (225), the handle shearing knife (223) is connected with the rod feeding sheet (226) in a relatively rotating mode through a shaft, the outer edge of the handle shearing knife (223) is provided with an arc, teeth are arranged on the arc, a handle shearing half gear (222) is meshed with the teeth at the outer edge of the handle shearing knife (223), and the handle shearing half gear (222) is connected with a motor;
a return spring (224) for returning the shank cutter (223) is also provided.
4. The system of claim 1, wherein the automated system comprises: the elution bottle conveying belt (20) adopts a chain plate type conveying belt, and a bottle seat (201) is arranged on the surface of a single chain plate and used for limiting the elution bottle (1);
the conveying belt (20) of the elution bottle is driven by a stepping motor.
5. The system of claim 4, wherein the system comprises: a vibrator (202) is arranged in the bottle seat (201), and the vibrator (202) vibrates the liquid in the elution bottle (1).
6. The system of claim 4, wherein the system comprises: the device is also provided with a bottle supply device (2), the bottle supply device (2) supplies the elution bottle (1) by adopting a rotary friction mechanism, and an eluent liquid supply device (27) is arranged on the path of the bottle supply and is used for supplying eluent to the elution bottle (1);
a notch corresponding to the bottle supplying device (2) is arranged on the bottle seat (201).
7. The system of claim 4, wherein the system comprises: the bottle cap separating device is further provided with a bottle cap supplying chain (3), chains (31) are arranged on two sides of the bottle cap supplying chain (3), a chain rod (32) is arranged in the middle of the bottle cap supplying chain, and the liquid separating bottle cap (4) is placed between the two chain rods (32).
8. The system of claim 6, wherein the system comprises: the test tube test device is characterized by also comprising test tube conveying belts (8), wherein the test tube conveying belts (8) are used for conveying test tube racks (7), each test tube rack (7) is internally provided with a storage test tube (25) and a cracking test tube (26) which are arranged in parallel, and a first pump (9) and a second pump (10) which are used for respectively injecting liquid into the storage test tube (25) and the cracking test tube (26) are arranged above the test tube conveying belts (8);
test tube conveyer belt (8) are equipped with closing device (13) from the below process of elution bottle conveyer belt (20) in the crisscross position of elution bottle conveyer belt (20) and test tube conveyer belt (8) for inject into respectively with the eluant in elution bottle (1) and preserve test tube (25) and schizolysis test tube (26).
9. The system of claim 8, wherein the automated system comprises: the structure of the liquid separating bottle cap (4) is as follows: a Y-shaped liquid separating joint (41) is arranged on the cover body (44), one end of the liquid separating joint (41) is connected with a liquid inlet pipe (42), the liquid inlet pipe (42) is positioned in the elution bottle (1), the other end of the liquid separating joint (41) is respectively connected with two liquid separating pipes (43), and the liquid separating pipes (43) are positioned outside the elution bottle (1);
the cover body (44) is also provided with a pressure column (45), and the pressure column (45) is in sealed sliding connection with the cover body (44);
the capping device (13) is provided with a capping cylinder (132) for pressing the plunger (45) down below the cap body (44).
10. The system of claim 8, wherein the automated system comprises: a first laser scanner (16) is arranged on the traveling path of the sampling brush conveyor belt (5);
a second laser scanner (15) is arranged on a bottle supplying path of the bottle supplying device (2);
a third laser scanner (14) is arranged on the conveying path of the test tube conveying belt (8);
the first laser scanner (16) is used for scanning the identification code of the sampling brush (24);
the second laser scanner (15) is used for scanning the identification code of the elution bottle (1);
the third laser scanner (14) is used for scanning the identification code of the test tube rack (7);
the corresponding identification codes are coded into a group, and the identification codes of the preservation test tube (25) and the cracking test tube (26) in the test tube rack (7) are also coded into a group with the identification code of the test tube rack (7).
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