CN113826624B - Application of methyl furoate as plant growth regulator, plant growth regulator and application method thereof - Google Patents
Application of methyl furoate as plant growth regulator, plant growth regulator and application method thereof Download PDFInfo
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- CN113826624B CN113826624B CN202111283302.0A CN202111283302A CN113826624B CN 113826624 B CN113826624 B CN 113826624B CN 202111283302 A CN202111283302 A CN 202111283302A CN 113826624 B CN113826624 B CN 113826624B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
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Abstract
The invention belongs to the technical field of agricultural production, and particularly discloses an application of methyl furoate as a plant growth regulator, a plant growth regulator and a use method thereof. The invention discloses a compound solution mainly composed of methyl furoate, sodium gluconate and water, wherein the concentration of the methyl furoate is 0.08-0.2 mg/mL, and the concentration of the sodium gluconate is 0.5-0.7 mg/mL. The solution has the growth regulating effect on plants, is sprayed for 1-4 times from the vegetative growth period to the initial reproductive growth period of the plants, can regulate the growth of the plants, obviously enlarges the leaf area, accelerates the accumulation of dry matters and improves the root-cap ratio.
Description
Technical Field
The invention relates to the technical field of agricultural production, in particular to an application of methyl furoate as a plant growth regulator, a plant growth regulator and a using method thereof.
Background
With the development of scientific technology and agricultural modernization, plant growth regulating substances are widely used in crop production, and especially play an important role in controlling plant growth and development. The regulation of plant growth by using plant growth regulator has been used as one of the main measures for realizing crop resistance and yield increase in 21 st century at home and abroad.
A large number of researches show that the plant growth regulator can be used for regulating and controlling the synthesis of nucleic acid, protein and enzyme in plants, and can play a role in regulating and controlling different stages of plant growth and development, such as germination, rooting, cell elongation, organ differentiation, flower bud differentiation, flowering, fruiting, leaf falling, dormancy and the like. In recent years, research and development of plant growth regulators are rapidly developed, and the plant growth regulators are widely applied to production of agricultural, forestry and horticultural crops, and especially play a great role in regulating plant growth and formation and distribution of nutrient substances, promoting individual growth and development, enhancing stress resistance of crops, increasing crop yield, improving product quality, improving planting benefits and the like. Meanwhile, the plant growth regulator can also play a role in improving the utilization efficiency of the fertilizer and the labor production efficiency, so the method is a technology with wide application prospect.
Methyl furoate (Methyl 2-furoate, pyromucic acid Methyl ester, 13), also known as Methyl 2-furoate, methyl 2-furancarboxylate, methyl pyromucate, etc. The natural smoke is in plants such as peanut, cocoa, coffee, roasted hazelnut, almond and rhizome of Lepidium meyenii (maca), and also in flue-cured tobacco leaves and various smoke gases (including smoke generated by charcoal production by biomass charcoal). Methyl 2-furoate is one of important fine chemical products converted from biomass, and has attracted extensive attention in the field of biomass conversion. The 2-furoic acid methyl ester is one of important derivatives in furan ring system compounds, the active center is carbonyl, the carbonyl has higher reactivity and larger polarity, and the molecules of the carbonyl have furan rings and aldehyde groups, so that furfural hydrogenation, oxidation, reductive amination, decarbonylation and other reactions are easy to occur. The characteristics determine that the methyl 2-furoate is an extremely important novel synthetic spice, occupies an important position in a synthetic spice family, and is widely applied to industries of food essence, tobacco essence, cosmetic essence and the like. Meanwhile, the product is an important chemical raw material and an intermediate, and can be used as a gasoline antiknock agent to improve the quality of gasoline. In the field of medicine, methyl furoate can also be used for preparing medicines with antitumor activity.
At present, no research and report related to the application of methyl furoate in promoting plant growth exists, and the development of the crop growth regulation application of methyl furoate is beneficial to agricultural high-efficiency production and biomass recycling.
Disclosure of Invention
In view of the above, the invention provides an application of methyl furoate as a plant growth regulator, a plant growth regulator and a use method thereof, innovatively provides an effect of methyl furoate on regulating and controlling crop growth and an effect thereof, and provides a new way for promoting agricultural high-efficiency production and recycling of biomass.
In order to achieve the purpose, the invention adopts the following technical scheme:
application of furoic acid methyl ester as plant growth regulator.
Another object of the present invention is to provide a plant growth regulator, which consists of methyl furoate, sodium gluconate and water;
wherein the concentration of the methyl furoate is 0.08-0.2 mg/mL, and the concentration of the sodium gluconate is 0.4-0.7 mg/mL.
It is still another object of the present invention to provide a method for using a plant growth regulator, which is applied from the vegetative growth stage to the reproductive growth stage of a plant.
Preferably, the plant growth regulator is sprayed on the foliage for 1 to 4 times at an interval of 5 to 7 days.
Preferably, the application amount of each spraying single plant is 2-8 mL.
Preferably, when the plant to be treated is a monocotyledonous cereal crop such as rice, the plant growth regulator is applied from the 3-leaf stage to the heading stage.
Preferably, when the plant to be treated is dicotyledonous crops such as cotton and rape, the plant growth regulator is applied from the 3-leaf stage to the initial flowering stage.
According to the technical scheme, compared with the prior art, the invention has the following beneficial effects:
the invention is based on the characteristic that the furoic acid methyl ester has low-concentration promotion and high-concentration inhibition effects on plants, can be used as a novel plant growth regulating component, and is used in the fields of agriculture, forestry, gardening and the like. The furoic acid methyl ester can regulate the growth of crops, effectively accelerate the accumulation of dry matters and improve the root-cap ratio. The crops can be monocotyledonous crops, and can also be dicotyledonous crops, such as rice, cotton, rape and the like.
Detailed Description
The invention provides an application of furoic acid methyl ester as a plant growth regulator.
The invention also provides a plant growth regulator, which consists of methyl furoate, sodium gluconate and water;
wherein, the concentration of the methyl furoate is 0.08-0.2 mg/mL, and the concentration of the sodium gluconate is 0.4-0.7 mg/mL. For monocotyledonous cereals such as rice, the concentration of the applied methyl furoate is preferably 0.11-0.16 mg/mL, and the concentration of the sodium gluconate is preferably 0.45-0.55 mg/mL; more preferably, the concentration of the methyl furoate is 0.13mg/mL, and the concentration of the sodium gluconate is 0.5mg/mL. For dicotyledonous crops such as cotton, rape and the like, the concentration of the applied methyl furoate is preferably 0.08-0.14 mg/mL, and the concentration of the sodium gluconate is preferably 0.55-0.65 mg/mL; more preferably, the concentration of the methyl furoate is 0.1mg/mL, and the concentration of the sodium gluconate is 0.6mg/mL.
The invention also provides a use method of the plant growth regulator, wherein the application period of the plant growth regulator is from vegetative growth period to reproductive growth period of the plant.
In the invention, the plant growth regulator is sprayed on the leaf surfaces for 1 to 4 times at an interval of 5 to 7 days; preferably, the spraying times are 4 times, and each spraying interval is 5 days.
In the invention, the application amount of each sprayed single plant is 2-8 mL; preferably, the application amount of each plant is 2-5 mL when the plant has 2-5 leaves; when the number of the plants is 6 or more, the amount applied to each plant is preferably 5 to 8mL.
In the present invention, when the plant to be affected is rice, the period of application of the plant growth regulator is from 3 leaves to heading, and the concentration of furfural methyl ester is preferably 0.1 to 0.2mg/mL.
In the present invention, when the plant to be treated is cotton or rape, the plant growth regulator is applied from the 3-leaf stage to the initial flowering stage, and the concentration of furfural methyl ester is preferably 0.08-0.13 mg/mL.
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
(1) Test materials: the test variety is the rice variety Huanghuazhan; rape variety Hua YOU ZAO No. 9 of cabbage type, cotton variety Hua Cotton 3097.
(2) The test treatment method comprises the following steps:
the rice is cultivated into young seedlings in a water culture mode. The rice seeds are sown in the self-made net-shaped floating water culture germination device box, the solution in the box is 1/4 Hoagland nutrient solution, 60 seeds are sown in each box, and the field planting is 30 plants after emergence of seedlings. Repeat 4 times.
The rape seedlings are cultivated by adopting a germination box and water culture mode. Seeds were first placed in a petri dish with wetted filter paper and germinated in the dark at 26 ℃. When the seed bud reaches 3-5 mm, the seed bud is placed on a 96-well plate paved with germination paper for growth. 60 seedlings with uniform growth conditions are selected, the cotyledonary nodes are clamped by sponge balls, and the seedlings are transferred to a 15mL centrifuge tube with 1/4 Hoagland nutrient solution to be continuously cultured in an incubator. Repeat 4 times, 15 strains per repeat.
The cotton is planted in planting boxes with length, width and height of 18cm,13cm and 8cm respectively, and nutrient soil is adopted: sand =1 mass ratio of substrate, sterilized by high temperature (130 ℃) and weighed 2kg per pot of substrate. 40 seeds are sowed in each box, and 12 plants are planted after emergence of seedlings. Repeat 4 times.
After sowing, the rice, rape and cotton were cultured in an intelligent light and temperature incubator (GDN-400, le electric instrument manufacturing ltd.). Incubator light (13000 lx), light/dark time set to 12h/12h. The night/day temperatures were set at 25/30 ℃ for rice, 22/28 ℃ for cotton and 20/25 ℃ for rape.
Spraying a plant growth regulator for the first time when 2-5 leaves (2 leaves of rape, 3 leaves of rice and 5 leaves of cotton) grow out of crop seedlings, wherein the application amount of each plant is 3ml of rape and rice and 5ml of cotton; spraying for the 2 nd time after 5d intervals, wherein the spraying dosage is the same as that of the first time, and spraying for the 3 rd time after 5d intervals, wherein the application dosage of each single plant is 5ml of rape and rice and 7ml of cotton; spray rinsing was used as Control (CK). When the foliage spraying treatment is carried out, the plant is taken out of the incubator and put into the incubator after the leaves are absorbed. When the crop is rice, the concentration of the methyl furoate solution in the plant growth regulator is 0.13mg/ml, and the concentration of the sodium gluconate is 0.5mg/ml; when the crop is cotton or rape, the concentration of the furoic acid methyl ester solution is 0.1mg/ml, and the concentration of the sodium gluconate is 0.6mg/ml. Sampling was investigated 2 weeks after spray application 3.
(3) The measuring and calculating method comprises the following steps: selecting 10 plants each time, respectively measuring the root length and stem length of seedlings by using a ruler, separately sampling and bagging the overground part and the underground part of each time, placing the bags in an oven for deactivation of enzymes at 105 ℃ for 20min, drying the bags at 70 ℃ to constant weight, respectively weighing the dry weight of stems and leaves and the dry weight of roots, taking an average value, and repeatedly taking the average value for many times.
The effect results of the methyl furoate treatment agent on seedling growth are shown in table 1, (lower case letters a, b, c, d, etc. represent significant differences, and P is less than 0.05).
TABLE 1
The data obtained from the detection in table 1 show that the plant height and the main root length of the experimental group applied with the plant growth regulator are obviously increased, the accumulation of dry matters is accelerated, and the root-cap ratio is improved.
Example 2
(1) Test materials: the test variety is cabbage type rape variety Hua YOU ZAO No. 9.
(2) Test treatment and method
The test was carried out in the potting field of the university of agriculture in Huazhong, wuhan, hubei. The pot culture method is adopted, and the diameter of a pot culture pot is 20cm, and the height of the pot culture pot is 18cm. The test soil is yellow brown loam, and the mass ratio of sand to soil is 1:1, each pot is filled with 4.5kg of soil. The main nutrient content of the soil to be tested sampled before sowing is 1.5mg/kg of ammoniacal nitrogen, 7.9mg/kg of nitrate nitrogen, 8.4mg/kg of available phosphorus, 112.9mg/kg of available potassium and 7.5mg/kg of organic matters, and the pH value of the soil is 6.0.
Sowing the seeds 10 and 19 days in 2020, and sowing 8 seeds in each pot. And (4) performing final singling in a three-leaf period, and keeping 4 healthy and strong seedlings with consistent growth vigor in each pot. 3g of N15-P15-K15 compound fertilizer is applied to each pot of base fertilizer. And (3) uniformly mixing the soil and the fertilizer before sowing, putting the mixture into a pot, and additionally applying 3g of compound fertilizer in each pot in the four-leaf period. The test sets 5 treatments (sequentially marked as J1, J2, J3, J4 and J5) of spraying for 1, 2, 3, 4, 5 times, etc., wherein after each spraying, the spraying is carried out again after 5 days. Each group was treated with 3 replicates.
The first foliar spraying is carried out by adopting 0.11mg/ml methyl furoate and 0.6mg/ml sodium gluconate mixed solution in 11 months and 12 days (three-leaf period) so as to spray clear water Control (CK). The spraying dosage is 5ml per plant. When spraying, the package box paperboard is adopted to block rape spraying and carry out adjacent treatment so as to ensure the accuracy of the concentration of the spraying agent. Other management technologies are carried out according to conventional cultivation measures. Sampling investigations were carried out on day 12, 20 after 5 spray treatments on day 12, 6 at 12 months to complete the last application.
(3) The measuring and calculating method comprises the following steps: 5 plants were randomly selected for each group, and the root length and stem length of the seedlings were measured with a ruler. Root neck thickness was measured using a vernier caliper. Leaf area was scanned and imaged with an Epson V800 scanner at 300dpi resolution and the Image was analyzed with Image J to calculate leaf area. Then, separately sampling and bagging each of the repeatedly overground part and the repeatedly underground part, placing the repeatedly overground part and the repeatedly underground part in an oven for deactivation of enzymes at 105 ℃ for 20min, drying the repeatedly overground part and the repeatedly overground part at 70 ℃ to a constant weight, and respectively weighing the dry weight of the stem leaves and the dry weight of the roots.
The results of the effect of foliar spray of the plant growth regulator on the growth of seedlings are shown in table 2 (the lower case letters a, b, c, d, e and f indicate that the LSD test has significant differences, and P is less than 0.05).
TABLE 2
The detection results in table 2 show that after the plant growth regulator determined by the invention is used for carrying out leaf surface treatment, the indexes of green leaf number, leaf area, stem leaf dry weight, root dry weight and total dry weight, root cap ratio, root neck thickness and the like of rape seedlings can reach obvious levels of treatment and contrast difference, and the plant growth regulator has the effects of obviously promoting growth and strengthening seedlings. The treatment agent can obviously promote the growth of plants after being sprayed for 1 to 4 times, the interval between the two spraying is preferably more than 5 days, and the best effect is achieved after 4 spraying.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (7)
1. Application of furoic acid methyl ester as plant growth regulator.
2. A plant growth regulator is characterized in that the plant growth regulator consists of methyl furoate, sodium gluconate and water;
wherein the concentration of the methyl furoate is 0.08-0.2 mg/mL, and the concentration of the sodium gluconate is 0.4-0.7 mg/mL.
3. The method of using a plant growth regulator according to claim 2, wherein the plant growth regulator is applied from vegetative growth to reproductive growth of the plant.
4. The use method of a plant growth regulator according to claim 3, wherein the plant growth regulator is used for foliar spraying, the spraying frequency is 1 to 4 times, and the interval between each spraying is 5 to 7 days.
5. The use method of a plant growth regulator according to claim 4, wherein the application amount of each sprayed plant is 2-8 mL.
6. The use of a plant growth regulator according to any one of claims 3 to 5, wherein the plant growth regulator is applied from the 3-leaf stage to the heading stage when the plant to be treated is a monocotyledonous cereal crop.
7. The use of a plant growth regulator according to any one of claims 3 to 5, wherein the plant growth regulator is applied from the 3-leaf stage to the initial flowering stage when the plant to be treated is a dicotyledonous crop.
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Non-Patent Citations (2)
| Title |
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| 喷施小分子有机物对小油菜生长发育和养分吸收的影响;于会丽 等;《植物营养与肥料学报》;20141125;第20卷(第6期);第1560-1568页 * |
| 螺环季酮酸类杀螨剂及新型植物生长调节剂的研制;关立婷;《中国优秀硕士学位论文全文数据库 (工程科技Ⅰ辑)》;20150415;B016-430 * |
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