CN113812547A - Feed for promoting growth of bee colonies and preparation method thereof - Google Patents
Feed for promoting growth of bee colonies and preparation method thereof Download PDFInfo
- Publication number
- CN113812547A CN113812547A CN202111106458.1A CN202111106458A CN113812547A CN 113812547 A CN113812547 A CN 113812547A CN 202111106458 A CN202111106458 A CN 202111106458A CN 113812547 A CN113812547 A CN 113812547A
- Authority
- CN
- China
- Prior art keywords
- feed
- powder
- bee
- parts
- promoting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 35
- 230000001737 promoting effect Effects 0.000 title claims abstract description 24
- 239000000843 powder Substances 0.000 claims abstract description 92
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 45
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 45
- 239000002994 raw material Substances 0.000 claims abstract description 40
- 235000015097 nutrients Nutrition 0.000 claims abstract description 32
- 235000000346 sugar Nutrition 0.000 claims abstract description 31
- 239000003623 enhancer Substances 0.000 claims abstract description 26
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 25
- 239000000839 emulsion Substances 0.000 claims abstract description 25
- 235000020251 goat milk Nutrition 0.000 claims abstract description 25
- 244000118350 Andrographis paniculata Species 0.000 claims abstract description 15
- 241000109365 Rosa arkansana Species 0.000 claims abstract description 15
- 235000005066 Rosa arkansana Nutrition 0.000 claims abstract description 15
- 108091005658 Basic proteases Proteins 0.000 claims abstract description 8
- 108010059345 keratinase Proteins 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 58
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 48
- 102000004190 Enzymes Human genes 0.000 claims description 38
- 108090000790 Enzymes Proteins 0.000 claims description 38
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims description 36
- 235000012000 cholesterol Nutrition 0.000 claims description 18
- 239000008367 deionised water Substances 0.000 claims description 18
- 229910021641 deionized water Inorganic materials 0.000 claims description 18
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 17
- 230000000415 inactivating effect Effects 0.000 claims description 17
- 229940083466 soybean lecithin Drugs 0.000 claims description 17
- 241000628997 Flos Species 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 15
- 235000015872 dietary supplement Nutrition 0.000 claims description 14
- 238000009835 boiling Methods 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 13
- 239000011324 bead Substances 0.000 claims description 12
- 150000001875 compounds Chemical class 0.000 claims description 12
- 239000011521 glass Substances 0.000 claims description 12
- 238000002390 rotary evaporation Methods 0.000 claims description 12
- 238000010008 shearing Methods 0.000 claims description 12
- 239000006228 supernatant Substances 0.000 claims description 12
- 238000005303 weighing Methods 0.000 claims description 12
- 150000002333 glycines Chemical class 0.000 claims description 11
- 210000000582 semen Anatomy 0.000 claims description 11
- 239000006210 lotion Substances 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 5
- 235000018102 proteins Nutrition 0.000 abstract description 39
- 241000257303 Hymenoptera Species 0.000 abstract description 34
- 238000009395 breeding Methods 0.000 abstract description 24
- 230000001488 breeding effect Effects 0.000 abstract description 24
- 230000000694 effects Effects 0.000 abstract description 8
- 239000000126 substance Substances 0.000 abstract description 7
- 235000019629 palatability Nutrition 0.000 abstract description 6
- 108010073771 Soybean Proteins Proteins 0.000 abstract description 5
- 230000000433 anti-nutritional effect Effects 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract description 4
- 235000021120 animal protein Nutrition 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 3
- 235000019710 soybean protein Nutrition 0.000 abstract description 3
- 239000003674 animal food additive Substances 0.000 abstract 1
- 241000256844 Apis mellifera Species 0.000 description 61
- 241000746375 Andrographis Species 0.000 description 34
- 229940088598 enzyme Drugs 0.000 description 32
- 238000012360 testing method Methods 0.000 description 20
- 241000256837 Apidae Species 0.000 description 14
- 230000000052 comparative effect Effects 0.000 description 11
- 240000004355 Borago officinalis Species 0.000 description 5
- 235000007689 Borago officinalis Nutrition 0.000 description 5
- 108010059892 Cellulase Proteins 0.000 description 5
- 229940106157 cellulase Drugs 0.000 description 5
- 235000020247 cow milk Nutrition 0.000 description 5
- 238000011161 development Methods 0.000 description 5
- 238000001035 drying Methods 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 239000002244 precipitate Substances 0.000 description 5
- 238000007873 sieving Methods 0.000 description 5
- 238000002791 soaking Methods 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 4
- 235000021050 feed intake Nutrition 0.000 description 4
- 235000019750 Crude protein Nutrition 0.000 description 3
- 235000010469 Glycine max Nutrition 0.000 description 3
- 244000068988 Glycine max Species 0.000 description 3
- 235000019764 Soybean Meal Nutrition 0.000 description 3
- 235000012907 honey Nutrition 0.000 description 3
- 239000002105 nanoparticle Substances 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 239000004455 soybean meal Substances 0.000 description 3
- 235000020357 syrup Nutrition 0.000 description 3
- 239000006188 syrup Substances 0.000 description 3
- PJVXUVWGSCCGHT-ZPYZYFCMSA-N (2r,3s,4r,5r)-2,3,4,5,6-pentahydroxyhexanal;(3s,4r,5r)-1,3,4,5,6-pentahydroxyhexan-2-one Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O.OC[C@@H](O)[C@@H](O)[C@H](O)C(=O)CO PJVXUVWGSCCGHT-ZPYZYFCMSA-N 0.000 description 2
- 102000011632 Caseins Human genes 0.000 description 2
- 108010076119 Caseins Proteins 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000782597 Hypericum erectum Species 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 238000009341 apiculture Methods 0.000 description 2
- 229940038481 bee pollen Drugs 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- GHVNFZFCNZKVNT-UHFFFAOYSA-N decanoic acid Chemical compound CCCCCCCCCC(O)=O GHVNFZFCNZKVNT-UHFFFAOYSA-N 0.000 description 2
- 239000003797 essential amino acid Substances 0.000 description 2
- 235000020776 essential amino acid Nutrition 0.000 description 2
- 230000037406 food intake Effects 0.000 description 2
- 235000012631 food intake Nutrition 0.000 description 2
- 208000006454 hepatitis Diseases 0.000 description 2
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 2
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 2
- 230000036737 immune function Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 229940001941 soy protein Drugs 0.000 description 2
- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- 240000003870 Ageratum houstonianum Species 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 241000256843 Apis mellifera ligustica Species 0.000 description 1
- 239000005632 Capric acid (CAS 334-48-5) Substances 0.000 description 1
- 239000005635 Caprylic acid (CAS 124-07-2) Substances 0.000 description 1
- 206010008909 Chronic Hepatitis Diseases 0.000 description 1
- 201000004624 Dermatitis Diseases 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 108700037728 Glycine max beta-conglycinin Proteins 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 238000007696 Kjeldahl method Methods 0.000 description 1
- 235000021314 Palmitic acid Nutrition 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 206010035503 Plasmodium vivax infection Diseases 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 241000202758 Scirpus Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241001072888 Teucrium Species 0.000 description 1
- 101710162629 Trypsin inhibitor Proteins 0.000 description 1
- 229940122618 Trypsin inhibitor Drugs 0.000 description 1
- 108010046377 Whey Proteins Proteins 0.000 description 1
- 102000007544 Whey Proteins Human genes 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000078 anti-malarial effect Effects 0.000 description 1
- 239000003430 antimalarial agent Substances 0.000 description 1
- 201000009361 ascariasis Diseases 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000037208 balanced nutrition Effects 0.000 description 1
- 235000019046 balanced nutrition Nutrition 0.000 description 1
- -1 bee pollen Substances 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 210000000038 chest Anatomy 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 150000002016 disaccharides Chemical class 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 230000003031 feeding effect Effects 0.000 description 1
- 108010083391 glycinin Proteins 0.000 description 1
- 235000021552 granulated sugar Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 208000021760 high fever Diseases 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000010150 least significant difference test Methods 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 201000004792 malaria Diseases 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000693 micelle Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 229960002446 octanoic acid Drugs 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000007065 protein hydrolysis Effects 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 235000020254 sheep milk Nutrition 0.000 description 1
- 150000004666 short chain fatty acids Chemical class 0.000 description 1
- 235000020374 simple syrup Nutrition 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 208000003265 stomatitis Diseases 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 229960004016 sucrose syrup Drugs 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
- 239000002753 trypsin inhibitor Substances 0.000 description 1
- 235000018648 unbalanced nutrition Nutrition 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000021119 whey protein Nutrition 0.000 description 1
- 235000021247 β-casein Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/168—Steroids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a feed for promoting bee colony growth and a preparation method thereof, belonging to the technical field of bee breeding. The feed raw materials comprise hydrolyzed protein powder, emulsion, wild rose pollen, powdered sugar, yeast tablets and nutrient enhancers. The invention takes hydrolyzed soybean protein as a main raw material, prepares a small amount of nutrient substances such as wild rose pollen, high-quality animal protein goat milk, powdered sugar and the like, uses the soybean protein which is fully enzymolyzed by keratinase and alkaline protease to effectively reduce the anti-nutritional factor level, uses fat-soluble substances to wrap the andrographis paniculata, can cover the bad smell of the andrographis paniculata, effectively improves the medicament stability of the andrographis paniculata, and reduces the bee colony morbidity. The raw materials are mutually matched and cooperate, so that the feed additive has good promotion effect on palatability of bee colonies and breeding of the bee colonies. The raw materials required by the invention are available on the market, the feeding method is simple and easy to implement, the popularization and the application are easy, the method is applied to large-scale bee farms, the high-quality culture of bees can be realized, and the economic benefit is obvious.
Description
Technical Field
The invention belongs to the technical field of bee breeding, and particularly relates to a feed for promoting bee colony growth and a preparation method thereof.
Background
With the acceleration of the urbanization process and the change of the ecological environment in recent years, the sources of the natural foods of the bees, namely pollen and nectar, face serious threats and greatly influence the health of bee colonies. The change of ecological environment can cause various problems such as single variety, reduced quantity, reduced quality and the like of plant pollen, the honey storage in the honeycomb is often insufficient, and bee colonies are often in the situations of insufficient eating and poor eating. Meanwhile, the nutritional composition in bee food directly affects the growth, development, life span, productivity and disease resistance of bee colony. Therefore, when the external honey powder source is deficient, the bee colony needs to be supplemented with appropriate artificial feed in time to maintain normal breeding of the bee colony. Based on the above practical situations, it is very important to develop artificial bee feed with high quality, low cost and strong palatability. The artificial bee feed is developed through scientific preparation and evaluation of feed and selection of proper feeding time on the premise of deep understanding of biological characteristics of bees and the nutritional composition and functions of bee food, so as to realize healthy breeding of bee colonies.
The bee artificial feed mainly comprises sugar feed and pollen-substituted feed. The sugar feed is generally used for replacing honey in a honeycomb and providing necessary carbohydrate for normal growth and reproduction of bee colonies, and the main sugar feed comprises white granulated sugar, syrup and the like. The pollen-substituted feed is a feed which can replace or partially replace pollen, has higher protein content and contains rich trace elements and vitamins.
Bee feed raw materials such as bee pollen, soybean meal or soybean meal powder, sucrose syrup, fructose-glucose syrup and the like are common in the market. However, bee pollen is high in price and has pathogenic bacteria hidden danger; the cane sugar syrup is not only high in price but also is not suitable for being fed by the bees who use the disaccharide sugar directly and is easy to be infected by the larvae disease; the soybean meal has anti-nutritional factors, so that the digestion and absorption of bees are influenced; the fructose-glucose syrup is low in price, but the actual feeding effect is poor, and the fatality rate of bees is high. The artificial feed has the problems of unbalanced nutrition, poor taste, poor functionality and the like, and the problems restrict the development of the beekeeping industry to the direction of high quality and high yield.
Generally, the development of bee feeds is mainly directed to pollen substitute feeds. Therefore, how to overcome the inherent defects of the current protein and saccharide beekeeping raw materials, solve the root problems, develop the artificial feed with balanced nutrition and good palatability, which can improve the immune function of the bees, promote the rapid and healthy growth and reproduction of the bees and is of great importance to the development of the bee industry.
Disclosure of Invention
The invention provides a high-efficiency artificial bee bread formula aiming at the problem of unobvious bee proliferation caused by insufficient natural bee bread and poor palatability of artificial bee bread in spring breeding and autumn breeding processes of bees at present, so that the feeding rate of bee colonies and the growth rate of the colony vigor are effectively improved, and the economic benefit is increased.
In order to achieve the technical purpose, the technical scheme adopted by the invention is as follows:
the feed for promoting bee colony growth is prepared from the following raw materials in parts by weight: 100-200 parts of hydrolyzed protein powder, 30-60 parts of emulsion, 20-30 parts of wild rose powder, 30-70 parts of powdered sugar, 1-6 parts of yeast tablet and 1-3 parts of nutrient enhancer.
Further, the preparation method of the hydrolyzed protein powder comprises the following steps: adding complex enzyme and deionized water into semen glycines powder, adjusting pH to 9-10, performing enzymolysis in water bath at constant temperature of 50 deg.C for 30-50min, inactivating enzyme in boiling water for 15min, centrifuging, collecting supernatant, concentrating, and lyophilizing to obtain hydrolyzed protein powder.
Further, the compound enzyme is formed by mixing keratinase and alkaline protease according to the mass ratio of 1:1, the adding amount of the compound enzyme is 3000U/g, and the using amount of deionized water is 1g/50 ml.
Further, the emulsion is goat milk.
Further, the preparation method of the nutrient enhancer comprises the following steps: weighing soybean lecithin and cholesterol according to the mass ratio of 1:1, adding an ethanol solution to dissolve the soybean lecithin and cholesterol, carrying out rotary evaporation at the rotating speed of 120r/min and the temperature of 50 ℃ to remove ethanol, adding an andrographis paniculata extract accounting for 20 wt% of the mass of the mixture, adding a proper amount of glass beads, rotating at the rotating speed of 120r/min and the temperature of 37 ℃, homogenizing the solution for 1min by a shearing machine after the solution is turbid, and homogenizing for 10 cycles under the pressure of 75MPa by a high-pressure homogenizer to obtain the nutrient supplement.
The preparation method of the common andrographis herb extract comprises the following steps: drying and crushing the common andrographis herb, sieving the dried and crushed common andrographis herb with a 60-mesh sieve to obtain common andrographis herb powder, adding water into the common andrographis herb powder, and uniformly stirring, wherein the weight ratio of the common andrographis herb powder to the water is 1: 7, soaking in water for 12 hours, adding cellulase for enzymolysis for 1 hour, inactivating at 100 ℃ for 10min, centrifuging at 4000r/min for 20min, separating, discarding precipitate to obtain herba Andrographitis extract
A preparation method of feed for promoting bee colony growth comprises the following steps:
(1) preparing hydrolyzed protein powder: adding complex enzyme and deionized water into soybean powder, adjusting pH to 9-10, performing enzymolysis in water bath at constant temperature of 50 deg.C for 30-50min, inactivating enzyme in boiling water for 15min, centrifuging, collecting supernatant, concentrating, and lyophilizing to obtain hydrolyzed protein powder;
(2) sterilizing flos Rosae Davuricae pollen and lotion respectively;
(3) respectively smashing the powdered sugar and the yeast tablets into powder for later use;
(4) preparing a nutrient enhancer: weighing soybean lecithin and cholesterol according to the mass ratio of 1:1, adding an ethanol solution for dissolving, carrying out rotary evaporation at the rotating speed of 120r/min and the temperature of 50 ℃ to remove ethanol, adding an andrographis paniculata extract accounting for 20 wt% of the mass of the mixture, adding glass beads, rotating at the rotating speed of 120r/min and the temperature of 37 ℃, homogenizing the solution for 1min by a shearing machine when the solution is turbid, and homogenizing for 10 cycles under the pressure of 75MPa by a high-pressure homogenizer to obtain a nutrient supplement;
(5) pouring sterilized flos Rosae Davuricae pollen into a container, adding mashed sugar and yeast powder, pouring sterilized emulsion, adding hydrolyzed protein powder and nutrient enhancer, and stirring.
After all the raw materials are uniformly mixed, the raw materials are kneaded into paste by hands, and a proper amount of goat milk can be supplemented in the preparation process to ensure the raw material forming. After the honey bees are evenly clustered, the honey bees are placed on the upper frame beam of the frame in the bee colony for feeding until the spring breeding and the autumn breeding are finished.
Each of the raw materials of the present invention is commercially available.
Wild rose pollen (Teucrium quatrifarium buch. -Ham.): scirpus tabulaefolius of family Bodinaceae or Hypericum erectum. The pollen has high quality and high quality in Yunnan province. Is a rose which is obtained by collecting pollen of plants of the family Flavonoidaceae in mountainous and semi-mountainous areas in 6-8 months by western bees, and the color of the removed pollen ball is golden yellow when the pollen ball is just dried in the sun. So it is called wild rose. Hypericum erectum plants are grown in villages, wastelands and shrubs and are distributed in Yunnan, Guangxi and other fields. The fruits and barks can be used as medicines, and have the effects of clearing away heat and toxic materials, dispelling wind and eliminating dampness, cooling blood and stopping bleeding, killing parasites, relieving itching, treating acute and chronic hepatitis, cold, dysentery, stomatitis, ascariasis, dermatitis, cough, antimalarial, tertian malaria, malignant malaria, cold, high fever, excessive phlegm and cough.
Goat milk has higher nutritive value than cow milk, and is known as 'king in milk'. The goat milk has low casein content, small micelle particles and strong beta-casein solubility; the whey protein content is high; and contains more mineral elements such as Ca, P, Mg, Cu and the like. The content of the essential amino acid in the goat milk is far higher than that in the cow milk. The sheep milk fat globules are about 3.5 μm in diameter, 1/2 for cow's milk. The goat milk has higher contents of caproic acid, caprylic acid, capric acid, palmitic acid, linoleic acid, triglyceride and the like than cow milk, and the content of short-chain fatty acid is 5 times of that of cow milk, so that the goat milk is easier to separate and absorb. Goat milk is the most suitable source of animal protein for bees.
The soybean has high crude protein content and balanced essential amino acid content, and is an important vegetable protein feed source. However, the anti-nutritional factors contained in soybean, such as trypsin inhibitor, glycinin, beta-conglycinin and oligosaccharide, seriously affect the absorption and utilization of nutrients.
Advantageous effects
The invention takes hydrolyzed soy protein as main raw material, prepares a small amount of nutrient substances such as wild rose pollen, high-quality animal protein goat milk, powdered sugar and the like, uses the soy protein which is fully enzymolyzed by keratinase and alkaline protease, effectively reduces the anti-nutritional factor level, improves the digestibility of crude protein and free amino acid in vivo, and the nutrient substances can be fully absorbed and utilized by bees. The addition of the wild rose pollen greatly improves the palatability of the bee bread and increases the food taking probability. In order to enhance the immune function of bees and improve the absorption rate of nutrient substances, fat-soluble lecithin and cholesterol are used for wrapping the extract of the common andrographis herb to prepare fat-soluble nano particles, and the fat-soluble substances are used for wrapping the common andrographis herb, so that the unpleasant odor of the common andrographis herb can be effectively covered, the stability of the common andrographis herb medicine can be effectively improved, the biocompatibility is good, the medicine effect can be continuously and effectively exerted in the bees, and the bee colony morbidity is reduced. Meanwhile, the existence of the nano particles can promote the uniform dispersion of the hydrolyzed protein, and the nano particles and the hydrolyzed protein are matched with each other and have synergistic effect, so that the palatability of the bee colony and the feeding and breeding of the bee colony are well promoted. The raw materials required by the invention are available on the market, the feeding method is simple and easy to implement, the popularization and the application are easy, the method is applied to large-scale bee farms, the high-quality culture of bees can be realized, and the economic benefit is obvious.
Detailed Description
The technical solution of the present invention is further described below with reference to specific embodiments, but is not limited thereto.
Example 1
The feed for promoting bee colony growth is prepared from the following raw materials in parts by weight: 100 parts of hydrolyzed protein powder, 30 parts of emulsion, 20 parts of wild rose pollen, 30 parts of powdered sugar, 1 part of yeast tablet and 1 part of nutrient enhancer.
The preparation method of the hydrolyzed protein powder comprises the following steps: adding complex enzyme and deionized water into semen glycines powder, and adjusting pH to 9-10
Performing enzymolysis in water bath at 50 deg.C for 30min, inactivating enzyme in boiling water for 15min, centrifuging, collecting supernatant, concentrating, and lyophilizing to obtain hydrolyzed protein powder.
The compound enzyme is prepared by mixing keratinase and alkaline protease according to the mass ratio of 1:1, the adding amount of the compound enzyme is 3000U/g, and the using amount of deionized water is 1g/50ml according to the solid-to-liquid ratio.
The emulsion is goat milk.
The preparation method of the nutrient enhancer comprises the following steps: weighing soybean lecithin and cholesterol according to the mass ratio of 1:1, adding an ethanol solution to dissolve the soybean lecithin and cholesterol, carrying out rotary evaporation at the rotating speed of 120r/min and the temperature of 50 ℃ to remove ethanol, adding an andrographis paniculata extract accounting for 20 wt% of the mass of the mixture, adding a proper amount of glass beads, rotating at the rotating speed of 120r/min and the temperature of 37 ℃, homogenizing the solution for 1min by a shearing machine after the solution is turbid, and homogenizing for 10 cycles under the pressure of 75MPa by a high-pressure homogenizer to obtain the nutrient supplement.
The preparation method of the common andrographis herb extract comprises the following steps: drying and crushing the common andrographis herb, sieving the dried and crushed common andrographis herb with a 60-mesh sieve to obtain common andrographis herb powder, adding water into the common andrographis herb powder, and uniformly stirring, wherein the weight ratio of the common andrographis herb powder to the water is 1: 7, soaking in water for 12 hours, adding cellulase for enzymolysis for 1 hour, inactivating at 100 ℃ for 10min, centrifuging at 4000r/min for 20min, separating, discarding precipitate to obtain herba Andrographitis extract
A preparation method of feed for promoting bee colony growth comprises the following steps:
(1) preparing hydrolyzed protein powder: adding complex enzyme and deionized water into semen glycines powder, adjusting pH to 9-10, performing enzymolysis in water bath at constant temperature of 50 deg.C for 30min, inactivating enzyme in boiling water for 15min, centrifuging, collecting supernatant, concentrating, and lyophilizing to obtain hydrolyzed protein powder;
(2) sterilizing flos Rosae Davuricae pollen and lotion respectively;
(3) respectively smashing the powdered sugar and the yeast tablets into powder for later use;
(4) preparing a nutrient enhancer: weighing soybean lecithin and cholesterol according to the mass ratio of 1:1, adding an ethanol solution for dissolving, carrying out rotary evaporation at the rotating speed of 120r/min and the temperature of 50 ℃ to remove ethanol, adding an andrographis paniculata extract accounting for 20 wt% of the mass of the mixture, adding glass beads, rotating at the rotating speed of 120r/min and the temperature of 37 ℃, homogenizing the solution for 1min by a shearing machine when the solution is turbid, and homogenizing for 10 cycles under the pressure of 75MPa by a high-pressure homogenizer to obtain a nutrient supplement;
(5) pouring sterilized flos Rosae Davuricae pollen into a container, adding mashed sugar and yeast powder, pouring sterilized emulsion, adding hydrolyzed protein powder and nutrient enhancer, and stirring.
The wild rose pollen can be sterilized by ultraviolet irradiation or high temperature cooking, and the goat milk can be sterilized by boiling fresh goat milk at high temperature and cooling.
After all the raw materials are uniformly mixed, the raw materials are kneaded into paste by hands, and a proper amount of goat milk can be supplemented in the preparation process to ensure the raw material forming. After the honey bees are evenly clustered, the honey bees are placed on the upper frame beam of the frame in the bee colony for feeding until the spring breeding and the autumn breeding are finished.
Example 2
The feed for promoting bee colony growth is prepared from the following raw materials in parts by weight: 150 parts of hydrolyzed protein powder, 50 parts of emulsion, 25 parts of wild rose pollen, 50 parts of powdered sugar, 4 parts of yeast tablets and 2 parts of nutrient enhancer.
The preparation method of the hydrolyzed protein powder comprises the following steps: adding complex enzyme and deionized water into semen glycines powder, and adjusting pH to 9-10
Performing enzymolysis in water bath at 50 deg.C for 40min, inactivating enzyme in boiling water for 15min, centrifuging, collecting supernatant, concentrating, and lyophilizing to obtain hydrolyzed protein powder.
The compound enzyme is prepared by mixing keratinase and alkaline protease according to the mass ratio of 1:1, the adding amount of the compound enzyme is 3000U/g, and the using amount of deionized water is 1g/50ml according to the solid-to-liquid ratio.
The emulsion is goat milk.
The preparation method of the nutrient enhancer comprises the following steps: weighing soybean lecithin and cholesterol according to the mass ratio of 1:1, adding an ethanol solution to dissolve the soybean lecithin and cholesterol, carrying out rotary evaporation at the rotating speed of 120r/min and the temperature of 50 ℃ to remove ethanol, adding an andrographis paniculata extract accounting for 20 wt% of the mass of the mixture, adding a proper amount of glass beads, rotating at the rotating speed of 120r/min and the temperature of 37 ℃, homogenizing the solution for 1min by a shearing machine after the solution is turbid, and homogenizing for 10 cycles under the pressure of 75MPa by a high-pressure homogenizer to obtain the nutrient supplement.
The preparation method of the common andrographis herb extract comprises the following steps: drying and crushing the common andrographis herb, sieving the dried and crushed common andrographis herb with a 60-mesh sieve to obtain common andrographis herb powder, adding water into the common andrographis herb powder, and uniformly stirring, wherein the weight ratio of the common andrographis herb powder to the water is 1: 7, soaking in water for 12 hours, adding cellulase for enzymolysis for 1 hour, inactivating at 100 ℃ for 10min, centrifuging at 4000r/min for 20min, separating, discarding precipitate to obtain herba Andrographitis extract
A preparation method of feed for promoting bee colony growth comprises the following steps:
(1) preparing hydrolyzed protein powder: adding complex enzyme and deionized water into semen glycines powder, adjusting pH to 9-10, performing enzymolysis in water bath at constant temperature of 50 deg.C for 40min, inactivating enzyme in boiling water for 15min, centrifuging, collecting supernatant, concentrating, and lyophilizing to obtain hydrolyzed protein powder;
(2) sterilizing flos Rosae Davuricae pollen and lotion respectively;
(3) respectively smashing the powdered sugar and the yeast tablets into powder for later use;
(4) preparing a nutrient enhancer: weighing soybean lecithin and cholesterol according to the mass ratio of 1:1, adding an ethanol solution for dissolving, carrying out rotary evaporation at the rotating speed of 120r/min and the temperature of 50 ℃ to remove ethanol, adding an andrographis paniculata extract accounting for 20 wt% of the mass of the mixture, adding glass beads, rotating at the rotating speed of 120r/min and the temperature of 37 ℃, homogenizing the solution for 1min by a shearing machine when the solution is turbid, and homogenizing for 10 cycles under the pressure of 75MPa by a high-pressure homogenizer to obtain a nutrient supplement;
(5) pouring sterilized flos Rosae Davuricae pollen into a container, adding mashed sugar and yeast powder, pouring sterilized emulsion, adding hydrolyzed protein powder and nutrient enhancer, and stirring.
After all the raw materials are uniformly mixed, the raw materials are kneaded into paste by hands, and a proper amount of goat milk can be supplemented in the preparation process to ensure the raw material forming. After the honey bees are evenly clustered, the honey bees are placed on the upper frame beam of the frame in the bee colony for feeding until the spring breeding and the autumn breeding are finished.
Example 3
The feed for promoting bee colony growth is prepared from the following raw materials in parts by weight: 200 parts of hydrolyzed protein powder, 60 parts of emulsion, 30 parts of wild rose pollen, 70 parts of powdered sugar, 6 parts of yeast tablets and 3 parts of nutrient enhancer.
The preparation method of the hydrolyzed protein powder comprises the following steps: adding complex enzyme and deionized water into semen glycines powder, and adjusting pH to 9-10
Performing enzymolysis in water bath at 50 deg.C for 50min, inactivating enzyme in boiling water for 15min, centrifuging, collecting supernatant, concentrating, and lyophilizing to obtain hydrolyzed protein powder.
The compound enzyme is prepared by mixing keratinase and alkaline protease according to the mass ratio of 1:1, the adding amount of the compound enzyme is 3000U/g, and the using amount of deionized water is 1g/50ml according to the solid-to-liquid ratio.
The emulsion is goat milk.
The preparation method of the nutrient enhancer comprises the following steps: weighing soybean lecithin and cholesterol according to the mass ratio of 1:1, adding an ethanol solution to dissolve the soybean lecithin and cholesterol, carrying out rotary evaporation at the rotating speed of 120r/min and the temperature of 50 ℃ to remove ethanol, adding an andrographis paniculata extract accounting for 20 wt% of the mass of the mixture, adding a proper amount of glass beads, rotating at the rotating speed of 120r/min and the temperature of 37 ℃, homogenizing the solution for 1min by a shearing machine after the solution is turbid, and homogenizing for 10 cycles under the pressure of 75MPa by a high-pressure homogenizer to obtain the nutrient supplement.
The preparation method of the common andrographis herb extract comprises the following steps: drying and crushing the common andrographis herb, sieving the dried and crushed common andrographis herb with a 60-mesh sieve to obtain common andrographis herb powder, adding water into the common andrographis herb powder, and uniformly stirring, wherein the weight ratio of the common andrographis herb powder to the water is 1: 7, soaking in water for 12 hours, adding cellulase for enzymolysis for 1 hour, inactivating at 100 ℃ for 10min, centrifuging at 4000r/min for 20min, separating, discarding precipitate to obtain herba Andrographitis extract
A preparation method of feed for promoting bee colony growth comprises the following steps:
(1) preparing hydrolyzed protein powder: adding complex enzyme and deionized water into semen glycines powder, adjusting pH to 9-10, performing enzymolysis in water bath at constant temperature of 50 deg.C for 50min, inactivating enzyme in boiling water for 15min, centrifuging, collecting supernatant, concentrating, and lyophilizing to obtain hydrolyzed protein powder;
(2) sterilizing flos Rosae Davuricae pollen and lotion respectively;
(3) respectively smashing the powdered sugar and the yeast tablets into powder for later use;
(4) preparing a nutrient enhancer: weighing soybean lecithin and cholesterol according to the mass ratio of 1:1, adding an ethanol solution for dissolving, carrying out rotary evaporation at the rotating speed of 120r/min and the temperature of 50 ℃ to remove ethanol, adding an andrographis paniculata extract accounting for 20 wt% of the mass of the mixture, adding glass beads, rotating at the rotating speed of 120r/min and the temperature of 37 ℃, homogenizing the solution for 1min by a shearing machine when the solution is turbid, and homogenizing for 10 cycles under the pressure of 75MPa by a high-pressure homogenizer to obtain a nutrient supplement;
(5) pouring sterilized flos Rosae Davuricae pollen into a container, adding mashed sugar and yeast powder, pouring sterilized emulsion, adding hydrolyzed protein powder and nutrient enhancer, and stirring.
After all the raw materials are uniformly mixed, the raw materials are kneaded into paste by hands, and a proper amount of goat milk can be supplemented in the preparation process to ensure the raw material forming. After the honey bees are evenly clustered, the honey bees are placed on the upper frame beam of the frame in the bee colony for feeding until the spring breeding and the autumn breeding are finished.
Comparative example 1
The feed for promoting bee colony growth is prepared from the following raw materials in parts by weight: 200 parts of hydrolyzed protein powder, 60 parts of emulsion, 30 parts of wild rose pollen, 70 parts of powdered sugar and 6 parts of yeast tablets.
The preparation method of the hydrolyzed protein powder comprises the following steps: adding complex enzyme and deionized water into semen glycines powder, and adjusting pH to 9-10
Performing enzymolysis in water bath at 50 deg.C for 50min, inactivating enzyme in boiling water for 15min, centrifuging, collecting supernatant, concentrating, and lyophilizing to obtain hydrolyzed protein powder.
The compound enzyme is prepared by mixing keratinase and alkaline protease according to the mass ratio of 1:1, the adding amount of the compound enzyme is 3000U/g, and the using amount of deionized water is 1g/50ml according to the solid-to-liquid ratio.
The emulsion is goat milk.
A preparation method of feed for promoting bee colony growth comprises the following steps:
(1) preparing hydrolyzed protein powder: adding complex enzyme and deionized water into semen glycines powder, adjusting pH to 9-10, performing enzymolysis in water bath at constant temperature of 50 deg.C for 50min, inactivating enzyme in boiling water for 15min, centrifuging, collecting supernatant, concentrating, and lyophilizing to obtain hydrolyzed protein powder;
(2) sterilizing flos Rosae Davuricae pollen and lotion respectively;
(3) respectively smashing the powdered sugar and the yeast tablets into powder for later use;
(4) pouring sterilized flos Rosae Davuricae pollen into container, adding mashed sugar and yeast powder, pouring sterilized emulsion, adding hydrolyzed protein powder, and stirring.
After all the raw materials are uniformly mixed, the raw materials are kneaded into paste by hands, and a proper amount of goat milk can be supplemented in the preparation process to ensure the raw material forming. After the honey bees are evenly clustered, the honey bees are placed on the upper frame beam of the frame in the bee colony for feeding until the spring breeding and the autumn breeding are finished.
This comparative example is the same as example 3 except that it does not contain a nutrient supplement.
Comparative example 2
The feed for promoting bee colony growth is prepared from the following raw materials in parts by weight: 200 parts of protein powder, 60 parts of emulsion, 30 parts of wild rose pollen, 70 parts of powdered sugar, 6 parts of yeast tablets and 3 parts of nutrient enhancer.
The emulsion is goat milk.
The preparation method of the nutrient enhancer comprises the following steps: weighing soybean lecithin and cholesterol according to the mass ratio of 1:1, adding an ethanol solution to dissolve the soybean lecithin and cholesterol, carrying out rotary evaporation at the rotating speed of 120r/min and the temperature of 50 ℃ to remove ethanol, adding an andrographis paniculata extract accounting for 20 wt% of the mass of the mixture, adding a proper amount of glass beads, rotating at the rotating speed of 120r/min and the temperature of 37 ℃, homogenizing the solution for 1min by a shearing machine after the solution is turbid, and homogenizing for 10 cycles under the pressure of 75MPa by a high-pressure homogenizer to obtain the nutrient supplement.
The preparation method of the common andrographis herb extract comprises the following steps: drying and crushing the common andrographis herb, sieving the dried and crushed common andrographis herb with a 60-mesh sieve to obtain common andrographis herb powder, adding water into the common andrographis herb powder, and uniformly stirring, wherein the weight ratio of the common andrographis herb powder to the water is 1: 7, soaking in water for 12 hours, adding cellulase for enzymolysis for 1 hour, inactivating at 100 ℃ for 10min, centrifuging at 4000r/min for 20min, separating, discarding precipitate to obtain herba Andrographitis extract
A preparation method of feed for promoting bee colony growth comprises the following steps:
(1) sterilizing flos Rosae Davuricae pollen and lotion respectively;
(2) respectively smashing the powdered sugar and the yeast tablets into powder for later use;
(3) preparing a nutrient enhancer: weighing soybean lecithin and cholesterol according to the mass ratio of 1:1, adding an ethanol solution for dissolving, carrying out rotary evaporation at the rotating speed of 120r/min and the temperature of 50 ℃ to remove ethanol, adding an andrographis paniculata extract accounting for 20 wt% of the mass of the mixture, adding glass beads, rotating at the rotating speed of 120r/min and the temperature of 37 ℃, homogenizing the solution for 1min by a shearing machine when the solution is turbid, and homogenizing for 10 cycles under the pressure of 75MPa by a high-pressure homogenizer to obtain a nutrient supplement;
(4) pouring sterilized flos Rosae Davuricae pollen into a container, adding mashed sugar and yeast powder, pouring sterilized emulsion, adding protein powder and nutrient enhancer, and stirring.
After all the raw materials are uniformly mixed, the raw materials are kneaded into paste by hands, and a proper amount of goat milk can be supplemented in the preparation process to ensure the raw material forming. After the honey bees are evenly clustered, the honey bees are placed on the upper frame beam of the frame in the bee colony for feeding until the spring breeding and the autumn breeding are finished.
This comparative example was the same as example 3 except that commercially available soybean protein powder was used as it is, i.e., no hydrolysis was performed.
Comparative example 3
The feed for promoting bee colony growth is prepared from the following raw materials in parts by weight: 200 parts of protein powder, 60 parts of emulsion, 30 parts of wild rose pollen, 70 parts of powdered sugar and 6 parts of yeast tablets.
The emulsion is goat milk.
A preparation method of feed for promoting bee colony growth comprises the following steps:
(1) sterilizing flos Rosae Davuricae pollen and lotion respectively;
(2) respectively smashing the powdered sugar and the yeast tablets into powder for later use;
(3) pouring sterilized flos Rosae Davuricae pollen into a container, adding mashed sugar and yeast powder, pouring sterilized emulsion, adding protein powder, and stirring.
After all the raw materials are uniformly mixed, the raw materials are kneaded into paste by hands, and a proper amount of goat milk can be supplemented in the preparation process to ensure the raw material forming. After the honey bees are evenly clustered, the honey bees are placed on the upper frame beam of the frame in the bee colony for feeding until the spring breeding and the autumn breeding are finished.
This comparative example was not subjected to hydrolysis of protein nor addition of nutrient enhancer, and the rest was the same as example 3.
Cultivation test
Test materials
The bee colony for western test of 2019 and 2020 is provided by the bee test demonstration bee field of the Bombycis mori bee research institute of agricultural academy of sciences of Yunnan province.
Test groups: feed obtained in examples 1 to 3 and comparative examples 1 to 3
Test time: the 1 st test time is 20 days in 8 months to 20 days in 10 months in 2019; the 2 nd (repeated) test time is 8 months, 15 days to 10 months, 25 days in 2020, and the period is the autumn breeding stage of local bee colony development, namely, the collected bees are cultivated in the florescence of the ageratum.
Test method
Selecting local Italian bee colonies 30 with substantially consistent queen bee breeding ability, colony worker bee number and quantum, and randomly dividing into 6 groups, and 5 groups.
Before the test is started, all bee colonies are checked one by one, and the situation of each bee colony is basically consistent. During the test period, pollen removers with small pore diameters are selected for all groups to control outworker bees to collect pollen, the test group feed is fed on a frame beam, the swarms are checked once every 6d, the swarms are ensured to be in a sufficient food state for a long time, the test pre-test period is 14d, the test period is 40d, and the total time is 54 d.
Index and method of measurement
Measuring the feed intake of the bee colony:
after the test is started, the feed is put on the frame beam in a cake shape for the honeybee to eat. And recording the feed intake of each group of bees, calculating the powder feed intake of each group of bees, replacing the feed every 3d, and covering a layer of preservative film above the powder cake to avoid water loss.
Determination of colony potential:
before the test is started, the colony potential of each group of bee colony is adjusted to ensure that the number of the cover sealing cover, the number of the larva spleens and the number of the adult bees are basically consistent.
Wherein, the group potential increase rate (%) (after-test group potential-before-test group potential)/before-test group potential × 100;
food intake rate (%). food intake/feed intake x 100
Measuring the primary weight of worker bees:
each colony of bees 10 just emerged from the house was placed in a centrifuge tube of known weight and weighed with an electronic balance (accurate to 0.0001g), and the average was recorded as the bee birth weight.
Measuring the activity of the intestinal related enzyme of the worker bees:
and respectively measuring the activities of the midgut protease and the amylase of the 7 d and 14d worker bees, measuring the absorbance value of each group by using an enzyme-labeling instrument, and calculating the activity of the enzyme according to the detected absorbance value of each group.
And (3) measuring the protein content of different parts of the worker bees:
separating the heads and the breasts of the 7 and 14d worker bees, removing the legs and the wings of the bees, and measuring the content of the crude protein by adopting a Kjeldahl method.
Statistical analysis
Single factor least squares analysis and multiple comparisons (LSD test) were performed using SPSS 11.5 statistical software.
The results of the experiment are shown in Table 1
TABLE 1 test results
The data in the table show that the population growth rate of the embodiment of the invention can reach 49.5%, the feeding rate is 99.6%, and the natural bee bread can be basically replaced. Worker bee birth weight is an important index for measuring individual performance of worker bees, the growth condition of worker bees can be directly influenced by nutrient components of different feeds, and the birth weight reflects the nutrient absorption and growth condition of bee larvae to a certain extent. The initial weight average of the worker bees is higher than that of the comparative example group, and the feed formula provided by the embodiment of the invention can also improve the feed quality to a certain extent and improve the quality of the nursing bees. The protein content of worker bees is an indirect reflection of the protein content of worker bees ingested, and the protein content of the heads and the chests of the group bees in the embodiment of the invention shows a higher value and is continuously increased. Compared with the comparative example 1 without the nutrition enhancer, the comparative example 2 without the proteolysis and the comparative example 3 without the two technical means, the protein utilization rate is far lower than that of the examples. Therefore, the raw materials of the invention are mutually matched and cooperate to promote the absorption and utilization of nutrient substances of bee colonies, and the effect is weak if the raw materials are not matched.
It should be noted that the above-mentioned embodiments are only some of the preferred modes for implementing the invention, and not all of them. Obviously, all other embodiments obtained by persons of ordinary skill in the art based on the above-mentioned embodiments of the present invention without any creative effort shall fall within the protection scope of the present invention.
Claims (6)
1. The feed for promoting bee colony growth is characterized by being prepared from the following raw materials in parts by weight: 100-200 parts of hydrolyzed protein powder, 30-60 parts of emulsion, 20-30 parts of wild rose powder, 30-70 parts of powdered sugar, 1-6 parts of yeast tablet and 1-3 parts of nutrient enhancer.
2. The feed for promoting bee colony growth according to claim 1, wherein the preparation method of the hydrolyzed protein powder comprises the following steps: adding complex enzyme and deionized water into semen glycines powder, adjusting pH to 9-10, performing enzymolysis in water bath at constant temperature of 50 deg.C for 30-50min, inactivating enzyme in boiling water for 15min, centrifuging, collecting supernatant, concentrating, and lyophilizing to obtain hydrolyzed protein powder.
3. The feed for promoting bee colony growth according to claim 2, wherein the compound enzyme is a mixture of keratinase and alkaline protease according to a mass ratio of 1:1, the addition amount of the compound enzyme is 3000U/g, and the usage amount of deionized water is 1g/50ml according to a solid-to-liquid ratio.
4. The feed for promoting the growth of bee colonies of claim 1, wherein the emulsion is goat milk.
5. The feed for promoting bee colony growth according to claim 1, wherein the nutrient supplement is prepared by the following steps: weighing soybean lecithin and cholesterol according to the mass ratio of 1:1, adding an ethanol solution for dissolving, carrying out rotary evaporation at the rotating speed of 120r/min and the temperature of 50 ℃ to remove ethanol, adding an andrographis paniculata extract accounting for 20 wt% of the mass of the mixture, adding glass beads, rotating at the rotating speed of 120r/min and the temperature of 37 ℃, homogenizing the solution for 1min by a shearing machine when the solution is turbid, and homogenizing for 10 cycles under the pressure of 75MPa by a high-pressure homogenizer to obtain the nutrient supplement.
6. A method for preparing the feed for promoting bee colony growth of any one of claims 1-5, comprising the steps of:
(1) preparing hydrolyzed protein powder: adding complex enzyme and deionized water into semen glycines powder, adjusting pH to 9-10, performing enzymolysis in water bath at constant temperature of 50 deg.C for 30-50min, inactivating enzyme in boiling water for 15min, centrifuging, collecting supernatant, concentrating, and lyophilizing to obtain hydrolyzed protein powder;
(2) sterilizing flos Rosae Davuricae pollen and lotion respectively;
(3) respectively smashing the powdered sugar and the yeast tablets into powder for later use;
(4) preparing a nutrient enhancer: weighing soybean lecithin and cholesterol according to the mass ratio of 1:1, adding an ethanol solution for dissolving, carrying out rotary evaporation at the rotating speed of 120r/min and the temperature of 50 ℃ to remove ethanol, adding an andrographis paniculata extract accounting for 20 wt% of the mass of the mixture, adding glass beads, rotating at the rotating speed of 120r/min and the temperature of 37 ℃, homogenizing the solution for 1min by a shearing machine when the solution is turbid, and homogenizing for 10 cycles under the pressure of 75MPa by a high-pressure homogenizer to obtain a nutrient supplement;
(5) pouring sterilized flos Rosae Davuricae pollen into container, adding mashed sugar powder and yeast powder, pouring sterilized emulsion, adding hydrolyzed protein powder and nutrient enhancer, and stirring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111106458.1A CN113812547B (en) | 2021-09-22 | 2021-09-22 | Feed for promoting growth of bee colony and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111106458.1A CN113812547B (en) | 2021-09-22 | 2021-09-22 | Feed for promoting growth of bee colony and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113812547A true CN113812547A (en) | 2021-12-21 |
| CN113812547B CN113812547B (en) | 2024-02-23 |
Family
ID=78915075
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111106458.1A Active CN113812547B (en) | 2021-09-22 | 2021-09-22 | Feed for promoting growth of bee colony and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113812547B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102138941A (en) * | 2011-03-31 | 2011-08-03 | 射阳县庆缘康蜂产品厂 | Precursor liposome of propolis flavone and preparation method thereof |
| CN104706694A (en) * | 2015-02-14 | 2015-06-17 | 浙江大学 | Pharmaceutical application of andrographis paniculata aqueous extract |
| CN106359305A (en) * | 2016-08-24 | 2017-02-01 | 覃政强 | Beekeeping method controlling bee crawling disease |
| CN107712483A (en) * | 2017-11-27 | 2018-02-23 | 安徽省甘泉养蜂专业合作社 | A kind of Chinese honeybee feed and preparation method thereof |
| CN107821335A (en) * | 2017-10-26 | 2018-03-23 | 南宁市浩发科技有限公司 | A kind of high-yield cultivation method of honeybee |
| CN108497222A (en) * | 2018-01-31 | 2018-09-07 | 东兰县百花园原生态养蜂场 | A kind of honeybee forage and preparation method thereof |
| CN110074285A (en) * | 2019-04-28 | 2019-08-02 | 安徽省农业科学院蚕桑研究所 | It is a kind of easily to absorb high nutrition honeybee forage and preparation method thereof |
-
2021
- 2021-09-22 CN CN202111106458.1A patent/CN113812547B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102138941A (en) * | 2011-03-31 | 2011-08-03 | 射阳县庆缘康蜂产品厂 | Precursor liposome of propolis flavone and preparation method thereof |
| CN104706694A (en) * | 2015-02-14 | 2015-06-17 | 浙江大学 | Pharmaceutical application of andrographis paniculata aqueous extract |
| CN106359305A (en) * | 2016-08-24 | 2017-02-01 | 覃政强 | Beekeeping method controlling bee crawling disease |
| CN107821335A (en) * | 2017-10-26 | 2018-03-23 | 南宁市浩发科技有限公司 | A kind of high-yield cultivation method of honeybee |
| CN107712483A (en) * | 2017-11-27 | 2018-02-23 | 安徽省甘泉养蜂专业合作社 | A kind of Chinese honeybee feed and preparation method thereof |
| CN108497222A (en) * | 2018-01-31 | 2018-09-07 | 东兰县百花园原生态养蜂场 | A kind of honeybee forage and preparation method thereof |
| CN110074285A (en) * | 2019-04-28 | 2019-08-02 | 安徽省农业科学院蚕桑研究所 | It is a kind of easily to absorb high nutrition honeybee forage and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113812547B (en) | 2024-02-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102204629B (en) | Feed additive and preparation method thereof | |
| CN101011114B (en) | Reinforced nutrient feed stuff for bee | |
| CN103315144A (en) | Biogas residue containing cow feed additive | |
| CN105707442A (en) | Method for preparing protein feed and protein feed additive by culturing fly maggots with defined medium | |
| CN103919077A (en) | Seed-melon full powder and preparing method thereof | |
| CN104957363A (en) | High-quality compound feed for adult turbots and preparation method thereof | |
| CN107319211A (en) | A kind of hirudinaria manillensis young perfect compound feed and preparation method thereof | |
| CN108201025A (en) | A kind of multifilament cray feed and preparation method thereof | |
| CN105876132A (en) | Preparation method of silkworm chrysalis-cordyceps militaris fermentation product feed additive | |
| CN101971972A (en) | Preparation method for simultaneously extracting Tibet barley serum and Tibet barley dietary fiber | |
| CN109892496B (en) | Bee feed composition and preparation method thereof | |
| CN109362983A (en) | A kind of selenium-rich chicken feed and preparation method thereof | |
| CN104472869A (en) | Mushroom bran protein biological feed additive and preparation method thereof | |
| CN103710237A (en) | Bee product wine and preparation method thereof | |
| CN107156509A (en) | A kind of duckling feed of Congyang matchmaker duck | |
| CN106360151A (en) | Feed additive for larvae of hypophthalmichthys molitrix | |
| CN114916626B (en) | Probiotic fish feed containing traditional Chinese medicine oligosaccharide and mulberry leaf oligosaccharide and application thereof | |
| CN104187198B (en) | A kind of yellow meal worm special compound feed and preparation method thereof | |
| CN104222694A (en) | Goose feed and production method thereof | |
| CN102987108B (en) | Vinasse duck feed and preparation method thereof | |
| CN102067951A (en) | Method for producing pollution-free selenium-enriched duck and duck egg by second conversion technology of selenium-enriched Chinese milk vetch | |
| CN105192311A (en) | Livestock and poultry compound feed additive | |
| CN113812547A (en) | Feed for promoting growth of bee colonies and preparation method thereof | |
| CN107080045A (en) | A kind of healthy pig feed of the cultivation containing chlorella and preparation method | |
| CN106962284A (en) | The feed and preparation method and the cultural method of chicken of a kind of chicken feed for being suitable to 13 week old |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |