CN113796387A - Method for preventing and treating myiasis of silkworms - Google Patents

Method for preventing and treating myiasis of silkworms Download PDF

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Publication number
CN113796387A
CN113796387A CN202111189588.6A CN202111189588A CN113796387A CN 113796387 A CN113796387 A CN 113796387A CN 202111189588 A CN202111189588 A CN 202111189588A CN 113796387 A CN113796387 A CN 113796387A
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fly
filter paper
silkworm
repelling
matrine
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CN113796387B (en
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陆琳
杨俊生
蔡国祥
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RUGAO SERICULTURE TECHNICAL GUIDANCE STATION
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/10Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
    • A01N57/12Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing acyclic or cycloaliphatic radicals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/34Shaped forms, e.g. sheets, not provided for in any other sub-group of this main group
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02Acyclic compounds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Toxicology (AREA)
  • Catching Or Destruction (AREA)

Abstract

The invention discloses a method for preventing and treating silkworm fly maggot disease, which comprises silkworm fly, matrine, n-butyl alcohol and filter paper, and is characterized in that: the fly-repelling filter paper is prepared by dripping 4-6 drops of 25% of silkworm fly, 2% of matrine and 99.9% of n-butyl alcohol on 8-12 cm-diameter filter paper according to the proportion of 0.8-1.2: 0.8-1.2, the fly-repelling filter paper is hung on an inlet of a silkworm rearing room and a frame of a ventilation window, a piece of fly-repelling filter paper is hung at each of four corners of the frame, and the fly-repelling filter paper is replaced after 46-52 hours. The prevention and control effect of the invention on the fly catching of silkworms is superior to that of the traditional fly killing additive, the fly expelling filter paper is manufactured, the use is more convenient, a large amount of manpower and material resources are saved, the invention is safe to people and livestock, the manufacturing raw materials are wide in source, the price is low, the preparation is easy, the environmental pollution is light, and the invention conforms to the current national industrial policy of low carbon, energy saving and environmental protection.

Description

Method for preventing and treating myiasis of silkworms
One, the technical field
The invention relates to a method for preventing and treating silkworm myiasis, and belongs to the technical field of chemical prevention and treatment of silkworm diseases.
Second, background Art
The silkworm fly maggot disease is an arthropod disease caused by the parasitism of silkworm fly hunting (Exorista sorbillans Wiedemann), the silkworm fly maggot disease is effectively controlled since the silkworm fly eradication is used for preventing and treating the silkworm fly maggot disease in 1963, but because the silkworm fly hunting is singly used for a long time, on one hand, the silkworm fly hunting has the drug resistance to the silkworm fly eradication, the prevention and treatment effect is reduced year by year, and the environmental pollution is caused, on the other hand, the silkworm fly maggot disease is caused by the fact that the silkworm fly hunting needs to be added or sprayed every other day after the silkworm leaves are opened, labor and time are wasted, and the silkworm is poisoned by careless attention when the pesticide is prepared, so that the economic benefit of silkworm production is influenced. The fly expelling filter paper is made up by using commercially available commodity pesticide and adjuvant as active components for expelling insects, so that it not only can raise the effect of expelling insects, but also has no irritant smell, and has no environmental pollution, and has no adverse effect on silkworm, human body and livestock.
Third, the invention
In order to overcome the defects of the prior art, medicaments which have small influence on silkworms, people and livestock and light environmental pollution are purchased from the market, the control effect on the fly catching on silkworms is verified one by one, more sensitive control medicaments are screened out and then are compounded with each other, so that the preferable component proportion combination is obtained. The technical scheme adopted by the invention is as follows: a method for preventing and treating silkworm myiasis comprises silkworm fly eradication, matrine, n-butyl alcohol and filter paper, and is characterized in that: the fly-repelling filter paper is prepared by dripping 4-6 drops of 25% of silkworm fly, 2% of matrine and 99.9% of n-butyl alcohol on 8-12 cm-diameter filter paper according to the proportion of 0.8-1.2: 0.8-1.2, the fly-repelling filter paper is hung at the entrance of a silkworm rearing room and the frame of a ventilation window, the fly-repelling filter paper is hung at the entrance and four corners of the frame of the ventilation window respectively, and the fly-repelling filter paper is replaced after 46-52 hours.
The invention provides a tested pesticide '25% silkworm fly killing' missible oil, which is produced by a stove silkworm pharmaceutical factory in Tokyo city (code number is A); the tested pesticide '2% matrine' aqua is a product of great industry biotechnology development limited company in Tianjin city, and the temporary registration certificate number of the pesticide is LS20090197 (the code number is B); n-butanol, chemical alcohol grade laboratory reagent, content 99.99% (code number C); ethanol, analytical pure-grade laboratory reagent, 99.7% (code number D); formaldehyde analysis pure-grade laboratory reagent, content 40.0% (code number is E). The medicaments are purchased from the market. The test silkworm fly-chasing imagoes are imagoes which are bred in an artificial room and eclose for two days, and the test silkworm variety is Su, Ju, Ming and Hu.
The formulation screening of the invention adopts a GB13917.4-92 method to carry out indoor efficacy test on the domestic silkworm fly-chasing flies. The tested insects are adults of two days after the emergence of the fly of the exosticum mori, and the male and female insects are tested by adopting a closed cylindrical device. The specific process is as follows: placing 30 test insects into a cylindrical glass cylinder with the diameter of 30cm and the height of 43cm from left to right, after the test insects recover normal activity, dropping a prepared medicament on filter paper according to a test design, placing the filter paper into the glass cylinder according to the degree of dropping moisture, moving the filter paper after 1 minute, recording the number of the knocked down test flies every other minute, transferring all test silkworm parasitic flies into a clean insect cage after 20 minutes, checking the number of dead insects after 24 hours, and repeating the treatment for 3 times.
The invention relates to a field simulation experiment, in particular to a closed environment experiment, wherein closed rooms with the same area are selected, the indoor area is about 18 square meters, 30 live flies are connected into each room, a control medicament group is sprayed for 5 seconds, doors and windows are closed after the ground is sprayed with wet air, and the doors and windows are opened for ventilation after 15 minutes. The number of knocked down insects was observed every 1 minute, and the number of dead test insects was checked 24 hours later. The fly-expelling filter paper group is prepared by dripping 5 drops of 25% of silkworm fly, 2% of matrine and 99.9% of n-butyl alcohol in a ratio of 1: 1 respectively, dripping wet filter paper, putting a piece of filter paper into a room to be tested, observing the number of knocked-down insects once every 1 minute, opening a door and a window after 15 minutes for ventilation, and checking the number of dead test insects after 24 hours. The two schemes of the open environment experiment are carried out in a ventilated and ventilated greenhouse, and the investigation method is the same as that of a closed room.
The production application test of the invention selects a plurality of farmers with similar silkworm breeding levels, the farmers are divided into two groups, the participating farmers use fly-expelling filter paper to expel flies, the contrast farmers use the fly-killing flies to feed by adopting the traditional method, cocoons are collected and randomly sampled and investigated after the silkworms bunch for 5 days, each family takes 100 cocoons, the incidence rate of myiasis, the total cocoon amount and the cocoon layer amount are investigated, the cocoon collection amount of each box of silkworm seeds is calculated, and the cocoon layer rate is calculated and repeated for three times.
The test results of the invention are as follows:
novel fly repellent formula screening test
The results of the screening test of the formula of the novel fly repellent are shown in table 1, considering the safety to silkworms, the selected medicaments are medicaments with lower toxicity to silkworms and human and livestock, the treatment No. 5-9 in the table is single-dose comparison, the treatment No. 1-3 in the table is binary compounding, and the treatment No. 4 in the table is ternary compounding, and the data in the table 1 show that the single dose has the best effect of killing silkworm flies, and then n-butyl alcohol and matrine, and the worse is ethanol and formaldehyde. In the binary compounding, the death rate of the combination of the silkworm fly and the n-butyl alcohol is the highest, the combination of the silkworm fly and the matrine is the second time, and the combination of the matrine and the n-butyl alcohol is the worst. The mortality rate of the Exorista civilis Rond in No. 4 treatment area (ternary complex area) is the highest, and the optimal combination is the shortest in the knock-down time KT50 (50%) and KT95 (95%) of the Exorista civilis Rond.
Table 1: fly-repelling medicament formula screening test result
Figure BSA0000254757040000031
Note: KT50 is the time for knocking down 50% of the tested insects, KT95 is the time for knocking down 95% of the tested insects, the content of the effective components for killing the silkworm flies is 25%, and the code is A; the code B is 2 percent of the content of the active ingredients of the matrine; n-butanol, the code number of which is C, the content of which is 99.99 percent. The ethanol content was 99.7% and the formaldehyde content was 40.0% and the code was D, the data in the table are the three-time repeated averages, "# is a significant difference from the statistical analysis of standard medication a, and the following table is the same.
Novel fly-repelling filter paper field simulation test result
The results of the novel fly-repelling filter paper field simulation test are shown in table 2, and the investigation data in table 2 shows that the novel fly-repelling medicament formula combination is superior to the conventional medicament silkworm fly killing spray control in the aspects of knockdown time and final control effect no matter in an airtight room experiment or an outdoor ventilation and light transmission environment experiment.
Table 2: novel fly-repelling filter paper field simulation test result
Figure BSA0000254757040000041
The novel fly-expelling filter paper is used for preventing and treating the silkworm fly maggot disease in production practice, the results are shown in table 3, and the investigation data in table 3 shows that the incidence rate of the silkworm fly maggot disease in a test area is obviously lower than that in a control area, and economic indexes such as the total cocoon quantity, the cocoon layer rate, the silkworm seed yield and the like are obviously higher than those in a conventional control area, so that the novel fly-expelling filter paper is feasible in production for preventing and treating the silkworm fly maggot disease.
Table 3: fly repelling agent production test.
Figure BSA0000254757040000042
In conclusion, the fly-repelling filter paper prepared by the novel fly-repelling medicament formula screened by the invention has better control effect on catching flies of silkworms than the traditional fly-killing additive, is more convenient to use, saves a large amount of manpower and material resources, is safe to people and livestock, has wide sources of preparation raw materials, low price, easy preparation and light environmental pollution, accords with the current national industrial policy of low carbon, energy conservation and environmental protection, and has certain market space if being developed into a new product to be pushed to the market.
Detailed description of the invention
Respectively taking 50 ml of 25% of silkworm fly, 2% of matrine and 99.9% of n-butyl alcohol, respectively packaging in three brown suction bottles, respectively sucking 5 drops of three medicaments according to the proportion of 1: 1 when the silkworm fly repellent liquid is used, dropping the three medicaments on 10 cm-diameter filter paper, hanging the filter paper on an inlet of a silkworm rearing room and a frame of a ventilation window, hanging one piece at each of four corners of the inlet of the silkworm rearing room and the frame of the ventilation window, and replacing the fly repellent filter paper after 48 hours.

Claims (3)

1. A method for preventing and treating silkworm myiasis comprises silkworm fly eradication, matrine, n-butyl alcohol and filter paper, and is characterized in that: dripping 4-6 drops of 25% of silkworm fly, 2% of matrine and 99.9% of n-butyl alcohol on 8-12 cm-diameter filter paper according to the proportion of 0.8-1.2: 0.8-1.2 respectively to prepare the fly-repelling filter paper which is ready for use.
2. The method for controlling silkworm maggot disease according to claim 1, characterized by that the fly-repelling filter paper is hung on the inlet of silkworm chamber and frame of air-permeable window.
3. The method for controlling silkworm fly maggot disease according to claim 2, characterized in that the fly-repelling filter paper is hung at the entrance of the silkworm room and at each of the four corners of the frame of the ventilating window, and the fly-repelling filter paper is replaced after 46-52 hours.
CN202111189588.6A 2021-09-27 2021-09-27 Method for preventing and treating myiasis of silkworms Active CN113796387B (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1070444A (en) * 1992-09-19 1993-03-31 阮祥林 The manufacture method of fly-killing paper
CN101138339A (en) * 2007-08-22 2008-03-12 浙江省农业科学院 Fume smoking sanitizer for sericultural production and preparing method thereof
CN101314774A (en) * 2008-05-07 2008-12-03 苏州大学 DNA extraction method for limited fragment length polymorphism research
CN102523995A (en) * 2010-12-28 2012-07-04 蔡国祥 New method for controlling mulberry pests
CN102772350A (en) * 2012-01-29 2012-11-14 宁波市鄞州福瑞达生物科技有限公司 External preparation for lightening fine hair color
CN103598203A (en) * 2013-11-04 2014-02-26 蔡国祥 Selective compound pesticide for controlling mulberry pests including diaphania pyloalis walker and bombyx mandarina leech

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1070444A (en) * 1992-09-19 1993-03-31 阮祥林 The manufacture method of fly-killing paper
CN101138339A (en) * 2007-08-22 2008-03-12 浙江省农业科学院 Fume smoking sanitizer for sericultural production and preparing method thereof
CN101314774A (en) * 2008-05-07 2008-12-03 苏州大学 DNA extraction method for limited fragment length polymorphism research
CN102523995A (en) * 2010-12-28 2012-07-04 蔡国祥 New method for controlling mulberry pests
CN102772350A (en) * 2012-01-29 2012-11-14 宁波市鄞州福瑞达生物科技有限公司 External preparation for lightening fine hair color
CN103598203A (en) * 2013-11-04 2014-02-26 蔡国祥 Selective compound pesticide for controlling mulberry pests including diaphania pyloalis walker and bombyx mandarina leech

Non-Patent Citations (1)

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Title
黄露等: "植物源杀虫剂苦参碱对家蚕的毒性试验", 《蚕业科学》 *

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Effective date of registration: 20231204

Address after: 226500 sericulture complex building, animal and plant testing center, Jiefang Road, Rucheng Town, Rugao City, Nantong City, Jiangsu Province

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Address before: 226500 sericulture station of Agricultural Service Center, Jiefang Road, Rucheng street, Rugao City, Nantong City, Jiangsu Province

Patentee before: Lu Lin