CN113755435B - 一种食蟹猴pbmc分离方法 - Google Patents
一种食蟹猴pbmc分离方法 Download PDFInfo
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- CN113755435B CN113755435B CN202111201413.2A CN202111201413A CN113755435B CN 113755435 B CN113755435 B CN 113755435B CN 202111201413 A CN202111201413 A CN 202111201413A CN 113755435 B CN113755435 B CN 113755435B
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- cynomolgus monkey
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- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 title claims abstract description 55
- 238000000926 separation method Methods 0.000 title claims abstract description 40
- 241000282567 Macaca fascicularis Species 0.000 title claims abstract description 27
- 210000004369 blood Anatomy 0.000 claims abstract description 35
- 239000008280 blood Substances 0.000 claims abstract description 35
- 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 claims abstract description 26
- 239000006228 supernatant Substances 0.000 claims abstract description 19
- 238000000034 method Methods 0.000 claims abstract description 15
- 238000000432 density-gradient centrifugation Methods 0.000 claims abstract description 11
- 210000005259 peripheral blood Anatomy 0.000 claims abstract description 9
- 239000011886 peripheral blood Substances 0.000 claims abstract description 9
- 238000005406 washing Methods 0.000 claims abstract description 8
- 239000013049 sediment Substances 0.000 claims abstract description 7
- 238000007865 diluting Methods 0.000 claims abstract description 4
- OZFAFGSSMRRTDW-UHFFFAOYSA-N (2,4-dichlorophenyl) benzenesulfonate Chemical compound ClC1=CC(Cl)=CC=C1OS(=O)(=O)C1=CC=CC=C1 OZFAFGSSMRRTDW-UHFFFAOYSA-N 0.000 claims abstract 8
- 229920001917 Ficoll Polymers 0.000 claims description 11
- 239000012530 fluid Substances 0.000 claims description 4
- 239000008188 pellet Substances 0.000 claims 2
- 238000005119 centrifugation Methods 0.000 abstract description 12
- 239000007788 liquid Substances 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 6
- 230000003833 cell viability Effects 0.000 abstract description 5
- 238000007796 conventional method Methods 0.000 abstract description 3
- 230000000630 rising effect Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 6
- 238000002955 isolation Methods 0.000 description 4
- 210000004698 lymphocyte Anatomy 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 238000013461 design Methods 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000000987 immune system Anatomy 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 108090000194 Dipeptidyl-peptidases and tripeptidyl-peptidases Proteins 0.000 description 1
- 102000003779 Dipeptidyl-peptidases and tripeptidyl-peptidases Human genes 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 210000005087 mononuclear cell Anatomy 0.000 description 1
- 230000007310 pathophysiology Effects 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
- C12N2509/10—Mechanical dissociation
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
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Abstract
Description
Claims (8)
Priority Applications (1)
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CN202111201413.2A CN113755435B (zh) | 2021-10-15 | 2021-10-15 | 一种食蟹猴pbmc分离方法 |
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CN202111201413.2A CN113755435B (zh) | 2021-10-15 | 2021-10-15 | 一种食蟹猴pbmc分离方法 |
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CN113755435A CN113755435A (zh) | 2021-12-07 |
CN113755435B true CN113755435B (zh) | 2023-10-13 |
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Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN113234673A (zh) * | 2021-05-14 | 2021-08-10 | 上海赛笠生物科技有限公司 | 一种优化的食蟹猴单个核细胞分离方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2011024575A (ja) * | 2009-07-01 | 2011-02-10 | Takara Bio Inc | 細胞の分離方法 |
WO2015181098A1 (en) * | 2014-05-28 | 2015-12-03 | F. Hoffmann-La Roche Ag | Antibodies binding to human and cynomolgus cd3 epsilon |
CN107254438A (zh) * | 2017-08-16 | 2017-10-17 | 妙顺(上海)生物科技有限公司 | 外周血单个核细胞的分离方法 |
CN113234673A (zh) * | 2021-05-14 | 2021-08-10 | 上海赛笠生物科技有限公司 | 一种优化的食蟹猴单个核细胞分离方法 |
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2021
- 2021-10-15 CN CN202111201413.2A patent/CN113755435B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2011024575A (ja) * | 2009-07-01 | 2011-02-10 | Takara Bio Inc | 細胞の分離方法 |
WO2015181098A1 (en) * | 2014-05-28 | 2015-12-03 | F. Hoffmann-La Roche Ag | Antibodies binding to human and cynomolgus cd3 epsilon |
CN107254438A (zh) * | 2017-08-16 | 2017-10-17 | 妙顺(上海)生物科技有限公司 | 外周血单个核细胞的分离方法 |
CN113234673A (zh) * | 2021-05-14 | 2021-08-10 | 上海赛笠生物科技有限公司 | 一种优化的食蟹猴单个核细胞分离方法 |
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Inventor after: Guo Xiaochen Inventor after: Jin Xiaoying Inventor after: Shi Jing Inventor after: Yang Hua Inventor after: Liu Lingfeng Inventor after: Zhu Chen Inventor after: Yu Yihong Inventor after: Wang Yajuan Inventor before: Guo Xiaochen Inventor before: Jin Xiaoying Inventor before: Shi Jing Inventor before: Yang Hua Inventor before: Liu Lingfeng Inventor before: Zhu Chen Inventor before: Yu Yihong Inventor before: Wang Yajuan |
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