CN113711867A - Method for improving seed production yield of wild watermelon - Google Patents

Method for improving seed production yield of wild watermelon Download PDF

Info

Publication number
CN113711867A
CN113711867A CN202111119150.0A CN202111119150A CN113711867A CN 113711867 A CN113711867 A CN 113711867A CN 202111119150 A CN202111119150 A CN 202111119150A CN 113711867 A CN113711867 A CN 113711867A
Authority
CN
China
Prior art keywords
wild
watermelon
wild watermelon
yield
forchlorfenuron
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202111119150.0A
Other languages
Chinese (zh)
Inventor
严蕾艳
王迎儿
古斌权
黄芸萍
邢乃林
王毓洪
张蕾琛
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ningbo Academy of Agricultural Sciences
Original Assignee
Ningbo Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ningbo Academy of Agricultural Sciences filed Critical Ningbo Academy of Agricultural Sciences
Priority to CN202111119150.0A priority Critical patent/CN113711867A/en
Publication of CN113711867A publication Critical patent/CN113711867A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/05Fruit crops, e.g. strawberries, tomatoes or cucumbers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/34Cucurbitaceae, e.g. bitter melon, cucumber or watermelon 
    • A01H6/342Citrullus lanatus [watermelon]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Physiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention provides a method for improving seed production yield of wild watermelons, which relates to the technical field of watermelon planting and comprises the following steps: collecting buds: collecting male flower buds of wild watermelon plants in a pollination period; artificial pollination: selecting female flowers blooming on a wild watermelon plant, and smearing male flower buds on female flower stigmas; spraying: spraying a forchlorfenuron and brassinolide compound preparation at the female flower melon embryo; calculating the yield: and (4) harvesting seeds after the wild watermelon is ripe, and calculating the yield. The invention compounds the forchlorfenuron and the brassinolide, and after the wild watermelon is artificially pollinated, the lima horse is uniformly sprayed on the watermelon embryo, so that the falling of the wild watermelon flower can be prevented, the fruit setting rate of the wild watermelon is improved, and the seed production yield is improved. The results of the embodiment show that after the wild watermelon is artificially pollinated in an auxiliary way and the forchlorfenuron and brassinolide compound liquid medicine is sprayed, the fruit setting rate of plants can reach 93.8 percent, and the seed yield per mu can reach 60.0 kg.

Description

Method for improving seed production yield of wild watermelon
Technical Field
The invention relates to the technical field of watermelon planting, in particular to a method for improving seed production yield of wild watermelons.
Background
With the continuous enlargement of the watermelon facility cultivation area, the increase of the soil multiple cropping index, the difficulty of crop rotation, the improper measures of soil disinfection and the like cause serious continuous cropping obstacles of the land, and the serious occurrence of soil-borne diseases such as blight and root rot becomes an important bottleneck for restricting the watermelon production. Watermelon grafting is one of the most effective measures for solving the major problem at present, wherein the selection of a good rootstock is the key of the measure. The wild watermelon has the characteristics of developed root system, strong growth potential, strong resistance and the like, and has high affinity with the cultivated watermelon. Therefore, it is widely used as a grafting stock for cultivating watermelon.
However, the wild watermelon has the problems of less female flowers, high natural death rate of flower buds, difficult fruit setting and the like, and the production requirement is difficult to meet due to low seed production yield.
Disclosure of Invention
In view of the above, the present invention aims to provide a method for increasing the seed production yield of wild watermelon. After the wild watermelon is artificially pollinated, the forchlorfenuron and brassinolide compound liquid medicine is quickly sprayed at the embryo of the female watermelon, so that the fruit setting rate of the wild watermelon can be improved, and the seed production yield of the wild watermelon is improved.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a method for improving seed production yield of wild watermelons, which comprises the following steps:
collecting buds: collecting male flower buds of wild watermelon plants in a pollination period;
artificial pollination: selecting female flowers blooming on a wild watermelon plant, and smearing male flower buds on female flower stigmas;
spraying: spraying a forchlorfenuron and brassinolide compound preparation at the female flower melon embryo;
calculating the yield: and (4) harvesting seeds after the wild watermelon is ripe, and calculating the yield.
Preferably, the effective component of the forchlorfenuron is 5-10 mu g-1
Preferably, the effective component of the brassinolide is 0.04-0.08 mu g.ml-1
Preferably, the volume ratio of the forchlorfenuron to the brassinolide in the compound medicament is 5: 1.
Preferably, the spraying amount of the compound medicament is 0.2-0.5 ml.
Preferably, the male flower buds are preserved in a moisture-preserving preservation box.
Preferably, after the male flower buds are smeared on the female flower stigmas, the forchlorfenuron and brassinolide compound preparation is sprayed rapidly.
The invention provides a method for improving seed production yield of wild watermelons, which comprises the following steps: collecting buds: collecting male flower buds of wild watermelon plants in a pollination period; artificial pollination: selecting female flowers blooming on a wild watermelon plant, and smearing male flower buds on female flower stigmas; spraying: spraying a forchlorfenuron and brassinolide compound preparation at the female flower melon embryo; calculating the yield: and (4) harvesting seeds after the wild watermelon is ripe, and calculating the yield. In the invention, the forchlorfenuron is a phenylurea plant growth regulator with cytokinin activity, and the problem that the male flower is lack or the artificial pollination process is complicated is solved by utilizing the substance to flower; brassinolide is a novel plant endogenous hormone, is a high-efficiency, broad-spectrum and non-toxic plant growth regulator, and can remarkably increase the growth of a plant nutrient body and promote fertilization at a very low concentration. The invention compounds the two medicaments of forchlorfenuron and brassinolide, and after the wild watermelon is artificially pollinated, the compound medicaments are uniformly sprayed on the watermelon embryo by the lima, so that the falling of wild watermelon flowers can be prevented, the fruit setting rate of the wild watermelon is improved, and the seed production yield is improved.
The results of the embodiment show that after the wild watermelon is artificially pollinated in an auxiliary way and the forchlorfenuron and brassinolide compound liquid medicine is sprayed, the fruit setting rate of plants can reach 93.8 percent, and the seed yield per mu can reach 60.0 kg.
Detailed Description
The invention provides a method for improving seed production yield of wild watermelons, which comprises the following steps: collecting buds: collecting male flower buds of wild watermelon plants in a pollination period; artificial pollination: selecting female flowers blooming on a wild watermelon plant, and smearing male flower buds on female flower stigmas; spraying: spraying a forchlorfenuron and brassinolide compound preparation at the female flower melon embryo; calculating the yield: and (4) harvesting seeds after the wild watermelon is ripe, and calculating the yield.
The invention firstly collects buds: in the pollination period, the male flower buds of wild watermelon plants are collected.
In the invention, the collected flower buds are preferably in the full bloom stage of the wild watermelon, and the time for collecting the flower buds is preferably in the morning of the pollination day.
In the present invention, the collected male flower buds are preferably stored in a moisture-retaining fresh keeping box.
After the male flower buds are obtained, the artificial pollination is carried out: selecting female flowers blooming on a wild watermelon plant, and smearing male flower buds on female flower stigmas.
In the invention, the smearing is preferably carried out by pressing and spraying for 1-2 times by using a small-sized spraying pot.
After artificial pollination is finished, the invention carries out spraying again: spraying a forchlorfenuron and brassinolide compound preparation at the female flower melon embryo.
In the invention, after the male flower bud is preferably smeared on the head of the female flower column, the forchlorfenuron and brassinolide compound preparation is quickly sprayed.
In the invention, the active ingredient of the forchlorfenuron is 5-10 mu g-1
The effective component of the brassinolide is 0.04-0.08 mu g.ml-1
The volume ratio of forchlorfenuron to brassinolide in the compound medicament is 5: 1.
the spraying amount of the compound medicament is 0.2-0.5 ml.
The source of the forchlorfenuron and the brassinolide is not specially limited, and the forchlorfenuron and the brassinolide are obtained by adopting a commercially available product. In the invention, the forchlorfenuron is a phenylurea plant growth regulator with cytokinin activity, and the problem that the male flower is lack or the artificial pollination process is complicated is solved by utilizing the substance to flower; the brassinolide is a novel plant endogenous hormone, is a high-efficiency, broad-spectrum and non-toxic plant growth regulator, and can remarkably increase the growth of a plant nutrient body and promote fertilization at a very low concentration. The invention compounds the forchlorfenuron and the brassinolide, and after the wild watermelon is subjected to conventional artificial pollination, the compound pesticide is uniformly sprayed on the watermelon embryo by the lima, so that the falling of the wild watermelon flower can be prevented, the fruit setting rate of the wild watermelon is improved, and the seed production yield is improved.
And (5) after the wild watermelon is ripe, harvesting seeds and calculating the yield.
The method for improving the seed production yield of wild watermelon provided by the invention is described in detail by the following examples, but the method is not to be construed as limiting the scope of the invention.
Example 1
Collecting buds: collecting male flower buds growing on wild watermelon plants in a centralized manner in the morning of pollination, and putting the flower buds into a moisture-preserving box;
artificial supplementary pollination: selecting bloomed female flowers on a wild watermelon plant, and smearing anthers of the male flowers on female flower stigmas;
spraying: quickly spraying forchlorfenuron (5 mu g.ml of effective component) on the female cucumber embryo-1) And brassinolide (effective component 0.04 μ g.ml)-1) Compounding the liquid medicine;
calculating the yield: and (4) harvesting seeds after the wild watermelon is ripe, and calculating the yield.
Comparative example 1
Collecting buds: collecting male flower buds growing on wild watermelon plants in a centralized manner in the morning of pollination, and putting the flower buds into a moisture-preserving box;
artificial supplementary pollination: and lightly smearing the male flower buds aiming at the female flower stigmas, uniformly smearing the male flower pollen on the female flower stigmas, harvesting seeds after the wild watermelons are mature, and calculating the yield.
Comparative example 2
Without the use of manpowerPollinating, directly spraying forchlorfenuron (5 μ g.ml of effective component) on the embryo position of female flower melon-1) And brassinolide (effective component 0.04 μ g.ml)-1) Compounding the liquid medicine, harvesting seeds after the wild watermelon is mature, and calculating the yield.
Comparative example 3
Collecting buds: collecting male flower buds growing on wild watermelon plants in a centralized manner in the morning of pollination, and putting the flower buds into a moisture-preserving box;
artificial supplementary pollination: selecting bloomed female flowers on a wild watermelon plant, and smearing anthers of the male flowers on female flower stigmas;
spraying: quickly spraying forchlorfenuron (5 mu g.ml of effective component) on the female cucumber embryo-1) Liquid medicine;
and (4) harvesting seeds after the wild watermelon fruits are ripe, and calculating the yield.
Comparative example 4
Collecting buds: collecting male flower buds growing on wild watermelon plants in a centralized manner in the morning of pollination, and putting the flower buds into a moisture-preserving box;
artificial supplementary pollination: selecting bloomed female flowers on a wild watermelon plant, and smearing anthers of the male flowers on female flower stigmas;
spraying: quickly spraying brassinolide (effective component 0.04 μ g.ml) onto embryo of female fructus Trichosanthis-1) Liquid medicine;
and (4) harvesting seeds after the wild watermelon fruits are ripe, and calculating the yield.
The specific mode for harvesting seeds and calculating the yield of the wild watermelon is as follows:
the calculation method comprises the following steps:
2 vines and pruning are adopted for wild watermelons, the mode of reserving the watermelons is one vine and one fruit, 320 plants are planted in each mu, the management modes of the embodiment 1 and the comparative examples 1-4 are the same, and the fruit setting rate and the seed yield of the wild watermelons are counted.
The fruit setting rate is the fruit quantity per mu/(320 x 2) 100%
Average seed yield of 10 fruits per acre 320 fruit set rate
The test results are shown in table 1:
TABLE 1 fruit set and seed yield for wild watermelon in example 1 and comparative examples 1-4
Treatment method Fruit setting rate% Seed yield kg/667m2
Example 1 93.8 60.0
Comparative example 1 14.3 10.1
Comparative example 2 78.1 0
Comparative example 3 70.3 49.5
Comparative example 4 54.7 33.6
Comparing example 1 with comparative examples 1-4, it can be seen that after artificial supplementary pollination is adopted for wild watermelon seed production, and forchlorfenuron and brassinolide compound liquid medicine are added, the fruit setting rate of plants is 93.8%, and the seed yield per mu is 60.0 kg; comparative example 1 adopts artificial supplementary pollination, the fruit setting rate of the wild watermelon is 14.3%, and the seed production yield per mu is 10.1 kg; comparative example 2 the fruit setting rate of wild watermelon directly sprayed with the forchlorfenuron and brassinolide compound liquid medicine without artificial pollination is 78.1%, but mature seeds are not obtained; comparative example 3 the fruit setting rate of spraying the forchlorfenuron liquid medicine after the artificial supplementary pollination mode is 70.3 percent, and the seed production yield per mu is 49.5 kg; comparative example 4 the fruit setting rate of spraying brassinolide liquid medicine after artificial supplementary pollination is 54.7%, and the seed production yield per mu is 33.6 kg. Experimental results show that after the wild watermelon is artificially pollinated in an auxiliary way, the forchlorfenuron and brassinolide compound liquid medicine is quickly sprayed at the embryo of the female watermelon, so that the fruit setting rate of the wild watermelon can be improved, and the seed production yield of the wild watermelon is improved.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (7)

1. The method for improving the seed production yield of the wild watermelon is characterized by comprising the following steps of:
collecting buds: collecting male flower buds of wild watermelon plants in a pollination period;
artificial pollination: selecting female flowers blooming on a wild watermelon plant, and smearing male flower buds on female flower stigmas;
spraying: spraying a forchlorfenuron and brassinolide compound preparation at the female flower melon embryo;
calculating the yield: and (4) harvesting seeds after the wild watermelon is ripe, and calculating the yield.
2. The method for improving seed production yield of wild watermelons according to claim 1, wherein the active ingredient of forchlorfenuron is 5-10 μ g-1
3. The method for improving seed production yield of wild watermelons according to claim 1, wherein the brassinolide has an active ingredient of 0.04-0.08μg.ml-1
4. The method for improving the seed production yield of the wild watermelon according to claim 1, wherein the volume ratio of the forchlorfenuron to the brassinolide in the compound medicament is 5: 1.
5. The method for improving seed production yield of wild watermelons according to claim 1, wherein the spraying amount of the compound pesticide is 0.2-0.5 ml.
6. The method for improving the seed production yield of the wild watermelon as claimed in claim 1, wherein the male flower buds are preserved in a moisture-preserving fresh-keeping box.
7. The method for improving the seed production yield of the wild watermelon as claimed in claim 1, wherein after the male flower bud is smeared on the head of the female flower column, the forchlorfenuron and brassinolide compound medicament is sprayed rapidly.
CN202111119150.0A 2021-09-24 2021-09-24 Method for improving seed production yield of wild watermelon Pending CN113711867A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111119150.0A CN113711867A (en) 2021-09-24 2021-09-24 Method for improving seed production yield of wild watermelon

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111119150.0A CN113711867A (en) 2021-09-24 2021-09-24 Method for improving seed production yield of wild watermelon

Publications (1)

Publication Number Publication Date
CN113711867A true CN113711867A (en) 2021-11-30

Family

ID=78684681

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202111119150.0A Pending CN113711867A (en) 2021-09-24 2021-09-24 Method for improving seed production yield of wild watermelon

Country Status (1)

Country Link
CN (1) CN113711867A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117204220A (en) * 2023-09-13 2023-12-12 扬州大学 Treatment method for improving seed production rate of cucumber seed melon
CN117204220B (en) * 2023-09-13 2024-06-11 扬州大学 Treatment method for improving seed production rate of cucumber seed melon

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103798130A (en) * 2014-02-11 2014-05-21 湖南生物机电职业技术学院 Watermelon hybrid seed production method
CN107258541A (en) * 2017-07-28 2017-10-20 韦荣昌 Promote the method for Momordica grosvenori UGT7 gene expressions
CN107360835A (en) * 2017-08-09 2017-11-21 广东日可威食品原料有限公司 A kind of implantation methods of selenium-rich casting skin wax gourd
CN112544383A (en) * 2020-12-09 2021-03-26 湖北省农业科学院中药材研究所 Ecological cultivation method for intercropping pinellia ternata and soybeans

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103798130A (en) * 2014-02-11 2014-05-21 湖南生物机电职业技术学院 Watermelon hybrid seed production method
CN107258541A (en) * 2017-07-28 2017-10-20 韦荣昌 Promote the method for Momordica grosvenori UGT7 gene expressions
CN107360835A (en) * 2017-08-09 2017-11-21 广东日可威食品原料有限公司 A kind of implantation methods of selenium-rich casting skin wax gourd
CN112544383A (en) * 2020-12-09 2021-03-26 湖北省农业科学院中药材研究所 Ecological cultivation method for intercropping pinellia ternata and soybeans

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117204220A (en) * 2023-09-13 2023-12-12 扬州大学 Treatment method for improving seed production rate of cucumber seed melon
CN117204220B (en) * 2023-09-13 2024-06-11 扬州大学 Treatment method for improving seed production rate of cucumber seed melon

Similar Documents

Publication Publication Date Title
CN112021122A (en) Method suitable for long-season cultivation of cowpeas
CN102754561A (en) Method for improving elymus nutans griseb seed yield in Aba county
CN111492924A (en) Accurate regulation and control method for peanuts
CN112679270A (en) Regulation and control pesticide fertilizer suitable for Xinjiang drip irrigation cotton and preparation method thereof
CN110915592A (en) Soybean cultivation method
CN113711867A (en) Method for improving seed production yield of wild watermelon
CN110720343B (en) Cultivation method for promoting differentiation of syzygium samarangense flower buds
CN114190223A (en) Fruit tree cultivation technology based on nitrogen-fixing blue algae and photosynthetic nutrient green algae
Ferree et al. Influence of Spray Adjuvants and Multiple Applications of Benomyl and Oil on Photosynthesis of Apple Leaves1
CN114304153A (en) Cold-resistant, strong and lodging-resistant plant growth regulator and application thereof to crops
RU2764263C1 (en) Method for cultivating winter rapeseed for seeds
Husain et al. Direct wet-seeded method of establishment of rice under irrigated condition
CN114631537B (en) Application of thidiazuron in preparation of red pepper collateral growth inhibitor
CN112753478B (en) Labor-saving fruit thinning method for facility cultivation of northern muscat grapes
LU501273B1 (en) Method for intercropping cotton with peanut
Vincent Vegetable and small fruit growing in toxic ex-orchard soils of central Washington
Dobrevska et al. Management practices for conventional apple production in the Plovdiv region.
RU2256328C1 (en) Treatment of grains with preparation larixin
Hopping et al. Effect of Ethrel 48 on sex expression and yield in pumpkins
Sagdiev et al. EFFECT OF GROWTH REGULATORY DRUGS ON CUCUMBER PRODUCTIVITY AND QUALITY
CN113575237A (en) Disease control method based on improvement of comprehensive resistance of tobacco
RU1806547C (en) Method for growing strawberries
CN111466410A (en) Plant growth regulator for cotton topping
Tripathi et al. 20 Use of Plant Bio-regulators
CN113647264A (en) Method for reducing wheat floret degeneration

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination