CN113693178A - Artificial feed for spruce flower-black longicorn larvae and preparation method thereof - Google Patents
Artificial feed for spruce flower-black longicorn larvae and preparation method thereof Download PDFInfo
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- CN113693178A CN113693178A CN202111039994.4A CN202111039994A CN113693178A CN 113693178 A CN113693178 A CN 113693178A CN 202111039994 A CN202111039994 A CN 202111039994A CN 113693178 A CN113693178 A CN 113693178A
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- 241000218657 Picea Species 0.000 title claims abstract description 44
- 238000002360 preparation method Methods 0.000 title abstract description 10
- 241001481710 Cerambycidae Species 0.000 title description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 28
- 239000000843 powder Substances 0.000 claims abstract description 28
- 240000007263 Pinus koraiensis Species 0.000 claims abstract description 21
- 235000011615 Pinus koraiensis Nutrition 0.000 claims abstract description 21
- 241001442207 Monochamus alternatus Species 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 17
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims abstract description 14
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims abstract description 14
- 229920001817 Agar Polymers 0.000 claims abstract description 14
- 102000011632 Caseins Human genes 0.000 claims abstract description 14
- 108010076119 Caseins Proteins 0.000 claims abstract description 14
- 235000019743 Choline chloride Nutrition 0.000 claims abstract description 14
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 14
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 14
- 229930006000 Sucrose Natural products 0.000 claims abstract description 14
- 241000209140 Triticum Species 0.000 claims abstract description 14
- 235000021307 Triticum Nutrition 0.000 claims abstract description 14
- 239000008272 agar Substances 0.000 claims abstract description 14
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract description 14
- 229960005070 ascorbic acid Drugs 0.000 claims abstract description 14
- 239000011668 ascorbic acid Substances 0.000 claims abstract description 14
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims abstract description 14
- 229960003178 choline chloride Drugs 0.000 claims abstract description 14
- 235000010241 potassium sorbate Nutrition 0.000 claims abstract description 14
- 239000004302 potassium sorbate Substances 0.000 claims abstract description 14
- 229940069338 potassium sorbate Drugs 0.000 claims abstract description 14
- 150000003839 salts Chemical class 0.000 claims abstract description 14
- 229940080237 sodium caseinate Drugs 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000012153 distilled water Substances 0.000 claims abstract description 13
- 235000002639 sodium chloride Nutrition 0.000 claims abstract description 9
- 235000018782 Dacrydium cupressinum Nutrition 0.000 claims abstract description 7
- 235000013697 Pinus resinosa Nutrition 0.000 claims abstract description 7
- 238000007873 sieving Methods 0.000 claims abstract description 7
- 239000005720 sucrose Substances 0.000 claims abstract description 7
- 238000001816 cooling Methods 0.000 claims abstract description 6
- 239000000203 mixture Substances 0.000 claims abstract description 6
- 238000012545 processing Methods 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 6
- 240000006055 Dacrydium cupressinum Species 0.000 claims abstract 2
- 229960004793 sucrose Drugs 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 4
- 238000007605 air drying Methods 0.000 claims description 2
- 241000254173 Coleoptera Species 0.000 claims 1
- 241001417495 Serranidae Species 0.000 claims 1
- 238000011161 development Methods 0.000 abstract description 12
- 241000238631 Hexapoda Species 0.000 abstract description 7
- 230000004083 survival effect Effects 0.000 abstract description 6
- 238000012360 testing method Methods 0.000 abstract description 6
- 239000002994 raw material Substances 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 230000018109 developmental process Effects 0.000 description 11
- 230000000052 comparative effect Effects 0.000 description 8
- 230000001954 sterilising effect Effects 0.000 description 8
- 240000005020 Acaciella glauca Species 0.000 description 6
- 241001442208 Monochamus Species 0.000 description 5
- 230000019617 pupation Effects 0.000 description 4
- 238000001035 drying Methods 0.000 description 3
- 230000032669 eclosion Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 230000001418 larval effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 241000243770 Bursaphelenchus Species 0.000 description 1
- 241000243771 Bursaphelenchus xylophilus Species 0.000 description 1
- 241000218641 Pinaceae Species 0.000 description 1
- 235000008582 Pinus sylvestris Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000008144 egg development Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000001839 pinus sylvestris Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
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Abstract
The invention discloses an artificial feed for spruce flower monochamus alternatus larvae and a preparation method thereof, and particularly relates to the field of insect feeding. The feed comprises Korean pine sawdust, agar, wheat germ powder, sucrose, sodium caseinate, yeast powder, ascorbic acid, weberian salt, potassium sorbate, choline chloride and distilled water. The method comprises the steps of processing Korean pine sawdust and then sieving; adding distilled water and prepared red pine sawdust, agar, wheat germ powder, sucrose, sodium caseinate, yeast powder, ascorbic acid, wegener salt, potassium sorbate and choline chloride into a container, and fully stirring to uniformly mix; and (5) naturally cooling after autoclaving to obtain the artificial feed for the spruce flower Monochamus alternatus larvae. The feed for artificially feeding the spruce sikka shook larvae, which is prepared by the invention, has the advantages of low raw material cost, simple preparation method, high survival rate of the larvae and short development time, is beneficial to realizing the continuous generation feeding of the spruce sikka shook larvae under the condition of artificial temperature control, and ensures the supply of test population of the spruce sikka shook larvae.
Description
Technical Field
The invention relates to the field of insect feeding, and particularly relates to an artificial feed for spruce flower monochamus alternatus larvae and a preparation method thereof.
Background
The spruce champion (Monochamus saltutorius) is a trunk-boring pest which mainly harms Pinus koraiensis, Pinus sylvestris, spruce and other pinaceae plants, more importantly, a main propagation medium of pine wood nematodes (Bursaphelenchus xylophilus) in intermediate temperature zones, the research reports on the champion are few at present, many related basic problems need to be researched definitely, and the method is not slow and has a great demand for testing insects.
The larvae and pupae stage of the spruce champion mainly move in xylem, the adult stage mainly moves on the upper part of the crown, and the collection difficulty is high. And forest collection is limited by climate, quantity, age and vitality, and the requirements of the test on the conditions of the age, the insect state and the like of the test longicorn cannot be guaranteed. Therefore, it is necessary to research the indoor artificial feeding method so as to provide materials for relevant experimental research.
Disclosure of Invention
Therefore, the invention provides an artificial feed for spruce flower monochamus larva and a preparation method thereof, and aims to solve the problem that the spruce flower monochamus larva cannot be further researched due to the lack of test insect materials in the prior art.
In order to achieve the above purpose, the invention provides the following technical scheme:
according to one aspect of the invention, the artificial feed for the spruce flower monochamus alternatus larva comprises Korean pine sawdust, agar, wheat germ powder, cane sugar, sodium caseinate, yeast powder, ascorbic acid, wegener salt, potassium sorbate, choline chloride and distilled water.
Further, the feed comprises 50-100 parts of Korean pine sawdust, 20-40 parts of agar, 12.5-30 parts of wheat germ powder, 10-30 parts of sucrose, 10-30 parts of sodium caseinate, 6-15 parts of yeast powder, 2-4 parts of ascorbic acid, 0.5-1 part of Webster salt, 0.5-1 part of potassium sorbate, 0.5-1 part of choline chloride and 800 parts of 400-ion water.
According to another aspect of the invention, a preparation method of an artificial feed for spruce flower monochamus alternatus larvae is provided, wherein the method comprises the following steps:
step one, processing Korean pine sawdust and then sieving;
adding distilled water and prepared red pine sawdust, agar, wheat germ powder, cane sugar, sodium caseinate, yeast powder, ascorbic acid, wechsler's salt, potassium sorbate and choline chloride into a container, and fully stirring to uniformly mix the materials;
and step three, naturally cooling the sterilized spruce flower monochamus longissimus larvae to obtain the artificial feed for the spruce flower monochamus longissimus larvae.
Further, in the first step, the method for processing the sawdust of the Korean pine is natural air drying.
Further, in the first step, the mesh number of the sieve is 20 meshes.
Further, in the third step, the high-pressure sterilization condition is 121 ℃ high-pressure sterilization pot.
Further, in the third step, the time for autoclaving is 20 minutes.
Further, in the third step, the temperature for low-temperature preservation is 4 ℃.
The invention has the following advantages:
the feed for artificially feeding spruce flower monochamus alternatus larvae, which is prepared by the invention, has low raw material cost and a simple preparation method. The survival rate of the larvae fed by the feed is up to 78.00 percent, the development time is short, the development period from egg development to eclosion is about 120 days, and the development time is obviously shortened compared with the development time under the natural environment of forests, so that the method is favorable for realizing the successive generation feeding of the spruce thyestillageblerifacldermann larvae under the artificial temperature control, and the test population supply of the spruce thyestillageblerifacldermann larvae with different insect ages and different insect states is ensured.
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1 an artificial feed for spruce flower Monochamus alternatus larvae and a preparing method thereof
A feed for artificially feeding spruce sikkera championii larvae comprises the following components: 50 parts of Korean pine sawdust, 20 parts of agar, 12.5 parts of wheat germ powder, 10 parts of cane sugar, 10 parts of sodium caseinate, 6 parts of yeast powder, 2 parts of ascorbic acid, 0.5 part of weberian salt, 0.5 part of potassium sorbate, 0.5 part of choline chloride and 800 parts of distilled water.
The method for preparing the feed comprises the following steps:
(1) naturally drying Korean pine sawdust, sieving with a 20-mesh sieve, and selecting Korean pine sawdust of less than 20 meshes;
(2) adding distilled water and prepared red pine sawdust, agar, wheat germ powder, sucrose, sodium caseinate, yeast powder, ascorbic acid, wegener salt, potassium sorbate, choline chloride and the like into a container, and fully stirring to uniformly mix the components;
(3) sterilizing in a high temperature sterilizing pot at 121 deg.C for 20 min, naturally cooling to obtain artificial feed for Caucaria spruce larva, and storing at 4 deg.C.
Example 2 Artificial feed for spruce flower Monochamus alternatus larvae and preparation method thereof
A feed for artificially feeding spruce sikkera championii larvae comprises the following components: 100 parts of Korean pine sawdust, 40 parts of agar, 30 parts of wheat germ powder, 30 parts of cane sugar, 30 parts of sodium caseinate, 15 parts of yeast powder, 4 parts of ascorbic acid, 1 part of webster salt, 1 part of potassium sorbate, 1 part of choline chloride and 800 parts of distilled water.
The method for preparing the feed comprises the following steps:
(1) naturally drying Korean pine sawdust, sieving with a 20-mesh sieve, and selecting Korean pine sawdust of less than 20 meshes;
(2) adding distilled water and prepared red pine sawdust, agar, wheat germ powder, sucrose, sodium caseinate, yeast powder, ascorbic acid, wegener salt, potassium sorbate, choline chloride and the like into a container, and fully stirring to uniformly mix the components;
(3) sterilizing in a high temperature sterilizing pot at 121 deg.C for 20 min, naturally cooling to obtain artificial feed for Caucaria spruce larva, and storing at 4 deg.C.
Example 3 Artificial feed for spruce flower Monochamus alternatus larvae and preparation method thereof
A feed for artificially feeding spruce sikkera championii larvae comprises the following components: 50 parts of Korean pine sawdust, 40 parts of agar, 25 parts of wheat germ powder, 20 parts of cane sugar, 20 parts of sodium caseinate, 12.5 parts of yeast powder, 4 parts of ascorbic acid, 1 part of webster salt, 1 part of potassium sorbate, 1 part of choline chloride and 400 parts of distilled water.
The method for preparing the feed comprises the following steps:
(1) naturally drying Korean pine sawdust, sieving with a 20-mesh sieve, and selecting Korean pine sawdust of less than 20 meshes;
(2) adding distilled water and prepared red pine sawdust, agar, wheat germ powder, sucrose, sodium caseinate, yeast powder, ascorbic acid, wegener salt, potassium sorbate, choline chloride and the like into a container, and fully stirring to uniformly mix the components;
(3) sterilizing in a high temperature sterilizing pot at 121 deg.C for 20 min, naturally cooling to obtain artificial feed for Caucaria spruce larva, and storing at 4 deg.C.
Comparative example
The natural Korean pine sawdust without processing is used as a comparative feed for artificially feeding the spruce flower Monochamus alternatus larvae.
Examples of the experiments
The average growth history of the spruce Monochamus alternatus on different feeds is shown in table 1 after recording and detection by breeding and comparison of the artificial feed and the comparative feed of the spruce Monochamus alternatus in the same conditions (the temperature is 25 +/-0.5 ℃ and the humidity is 60 +/-5%) respectively. The larval stages of rearing with example 1, example 2, example 3 and the comparative example averaged 128 days, 103 days, 117 days and 154 days, respectively; the pupation period of the larvae bred by the 4 feeds after pupation is 12 days, 7 days, 10 days and 15 days respectively. The development time of the larvae fed with the feed of example 2 was the shortest, the development duration from the development of the eggs to the emergence of pupae was about 120 days, which was significantly shorter than the development time in the natural environment of the forest (about 270 days), and also significantly different from the comparative example (about 180 days) fed with pure red pine wood chips.
TABLE 1 average growth period of spruce spotted longicorn cattle on different feeds
| Feed formula | Egg period (Tian) | Larval stage (Tian) | Pupal stage (sky) |
| Example 1 | 7 | 128 | 12 |
| Example 2 | 7 | 103 | 7 |
| Example 3 | 7 | 117 | 10 |
| Comparative example | 7 | 154 | 15 |
The survival rate and the development condition of the spruce monochamus alternatus on different feeds are shown in table 2. The survival rates of larvae reared with the feeds of example 1, example 2, example 3 and comparative example were 62.00%, 78.00%, 71.00% and 53.00%, respectively; pupation rates are respectively 38.71%, 69.23%, 50.70% and 35.70%; the emergence rates were 79.17%, 85.19%, 80.56% and 78.95%, respectively; the mass of the eclosion adult is 0.23g, 0.41g, 0.35g and 0.20g respectively. It is seen that the feed of example 2 had the best development of larvae.
TABLE 2 survival rate and development of spruce spotted longicorn cattle on different feeds
| Feed formula | Survival Rate (%) of larva | Pupation Rate (%) | Eclosion Rate (%) | Adult quality (g) |
| Example 1 | 62.00 | 38.71 | 79.17 | 0.23 |
| Example 2 | 78.00 | 69.23 | 85.19 | 0.41 |
| Example 3 | 71.00 | 50.70 | 80.56 | 0.35 |
| Comparative example | 53.00 | 35.85 | 78.95 | 0.20 |
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (8)
1. An artificial feed for spruce champion larvae is characterized by comprising Korean pine sawdust, agar, wheat germ powder, sucrose, sodium caseinate, yeast powder, ascorbic acid, wechsler salt, potassium sorbate, choline chloride and distilled water.
2. The artificial feed for spruce champion larvae is characterized in that the feed comprises 50-100 parts of Korean pine sawdust, 20-40 parts of agar, 12.5-30 parts of wheat germ powder, 10-30 parts of cane sugar, 10-30 parts of sodium caseinate, 6-15 parts of yeast powder, 2-4 parts of ascorbic acid, 0.5-1 part of weber's salt, 0.5-1 part of potassium sorbate, 0.5-1 part of choline chloride and 400-800 parts of distilled water.
3. A method of preparing an artificial feed for spruce grouper larvae as claimed in claim 1 or 2, comprising the steps of:
step one, processing Korean pine sawdust and then sieving;
adding distilled water and prepared red pine sawdust, agar, wheat germ powder, cane sugar, sodium caseinate, yeast powder, ascorbic acid, wechsler's salt, potassium sorbate and choline chloride into a container, and fully stirring to uniformly mix the materials;
and step three, naturally cooling after autoclaving to obtain the artificial feed for the spruce flower monochamus alternatus larvae, and storing at low temperature.
4. The method for preparing the artificial feed for the larvae of the spruce Monochamus alternatus according to claim 3, wherein in the first step, the method for processing the sawdust of the Korean pine is natural air drying.
5. The method for preparing the artificial feed for the larvae of the spruce Monochamus alternatus according to claim 3, wherein in the first step, the sieving mesh is 20 meshes.
6. The method for preparing the artificial feed for the larvae of the spruce Monochamus alternatus according to claim 3, wherein in the third step, the autoclaving condition is 121 ℃ autoclaving.
7. The method for preparing artificial feed for spruce champion larvae, according to claim 3, wherein in step three, the autoclaving time is 20 minutes.
8. The method for preparing artificial feed for spruce silky beetle larvae according to claim 3, wherein in the third step, the low-temperature storage temperature is 4 ℃.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111039994.4A CN113693178A (en) | 2021-09-06 | 2021-09-06 | Artificial feed for spruce flower-black longicorn larvae and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111039994.4A CN113693178A (en) | 2021-09-06 | 2021-09-06 | Artificial feed for spruce flower-black longicorn larvae and preparation method thereof |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101228853A (en) * | 2007-01-23 | 2008-07-30 | 中国林业科学研究院森林生态环境与保护研究所 | Artificial feeding and culture transfer of monochamus alternatus hope and artificial feed for larva thereof |
| CN101983580A (en) * | 2010-10-25 | 2011-03-09 | 南京林业大学 | Forage for artificial breeding of Monochamus alternatus Hope and method for preparing the same |
| CN110692600A (en) * | 2018-07-10 | 2020-01-17 | 中国林业科学研究院森林生态环境与保护研究所 | Artificial feed for anoplophora chinensis larvae and subculturing method of anoplophora chinensis |
| CN112471368A (en) * | 2020-11-04 | 2021-03-12 | 湖北省农业科学院植保土肥研究所 | Artificial feed for cowpea pod borer larvae and preparation method thereof |
-
2021
- 2021-09-06 CN CN202111039994.4A patent/CN113693178A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101228853A (en) * | 2007-01-23 | 2008-07-30 | 中国林业科学研究院森林生态环境与保护研究所 | Artificial feeding and culture transfer of monochamus alternatus hope and artificial feed for larva thereof |
| CN101983580A (en) * | 2010-10-25 | 2011-03-09 | 南京林业大学 | Forage for artificial breeding of Monochamus alternatus Hope and method for preparing the same |
| CN110692600A (en) * | 2018-07-10 | 2020-01-17 | 中国林业科学研究院森林生态环境与保护研究所 | Artificial feed for anoplophora chinensis larvae and subculturing method of anoplophora chinensis |
| CN112471368A (en) * | 2020-11-04 | 2021-03-12 | 湖北省农业科学院植保土肥研究所 | Artificial feed for cowpea pod borer larvae and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 岳书奎: "《天敌昆虫学》", 31 December 1993, 东北林业大学出版社 * |
| 潘佳亮等: "云杉花墨天牛取食倾向初报", 《中国森林病虫》 * |
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