CN113692446B - 用于鉴定黑果越橘的方法和试剂盒 - Google Patents
用于鉴定黑果越橘的方法和试剂盒Info
- Publication number
- CN113692446B CN113692446B CN202080029311.4A CN202080029311A CN113692446B CN 113692446 B CN113692446 B CN 113692446B CN 202080029311 A CN202080029311 A CN 202080029311A CN 113692446 B CN113692446 B CN 113692446B
- Authority
- CN
- China
- Prior art keywords
- seq
- dna
- blueberry
- plant
- black
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/08—Fruits
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/36—Ericaceae, e.g. azalea, cranberry or blueberry
- A01H6/368—Vaccinium, e.g. cranberry, blueberry
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2561/00—Nucleic acid detection characterised by assay method
- C12Q2561/113—Real time assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP19169555 | 2019-04-16 | ||
| EP19169555.0 | 2019-04-16 | ||
| PCT/EP2020/059807 WO2020212190A1 (en) | 2019-04-16 | 2020-04-06 | Method and kit for the identification of vaccinium myrtillus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113692446A CN113692446A (zh) | 2021-11-23 |
| CN113692446B true CN113692446B (zh) | 2026-03-17 |
Family
ID=66217789
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202080029311.4A Active CN113692446B (zh) | 2019-04-16 | 2020-04-06 | 用于鉴定黑果越橘的方法和试剂盒 |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US12571056B2 (https=) |
| EP (1) | EP3956455B1 (https=) |
| JP (1) | JP7578608B2 (https=) |
| KR (1) | KR102926935B1 (https=) |
| CN (1) | CN113692446B (https=) |
| AU (1) | AU2020260318A1 (https=) |
| BR (1) | BR112021020571A2 (https=) |
| CA (1) | CA3136865A1 (https=) |
| DK (1) | DK3956455T5 (https=) |
| ES (1) | ES2960514T3 (https=) |
| FI (1) | FI3956455T3 (https=) |
| HU (1) | HUE063576T2 (https=) |
| IL (1) | IL287205B1 (https=) |
| MX (1) | MX2021012714A (https=) |
| PL (1) | PL3956455T3 (https=) |
| PT (1) | PT3956455T (https=) |
| SG (1) | SG11202111379TA (https=) |
| SI (1) | SI3956455T1 (https=) |
| WO (1) | WO2020212190A1 (https=) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1823165A (zh) * | 2003-05-16 | 2006-08-23 | 家庭食品株式会社 | 食物或食物原材料中特定属的植物的定量pcr检测方法 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1052429A (zh) | 1990-09-15 | 1991-06-26 | 刘绍文 | 强心健脑液的配方、制作 |
| ES2176066B1 (es) | 2000-02-02 | 2003-12-16 | Univ Alcala Henares | Identificacion de adulteraciones vegetales por reaccion en cadena de la polimerasa (pcr) de acidos nucleicos. |
| CN1156587C (zh) | 2001-08-03 | 2004-07-07 | 中国药科大学 | 中国石斛属植物及其药材的dna分子鉴定方法 |
| WO2006020147A2 (en) | 2004-07-28 | 2006-02-23 | The Regents Of The University Of California | Use of pcr-based techniques to analyze compositions of botanicals |
| JP4928853B2 (ja) | 2006-03-24 | 2012-05-09 | ハウス食品株式会社 | キウイ類検出用プライマーセット |
| JP5625211B2 (ja) | 2009-12-21 | 2014-11-19 | 独立行政法人農業・食品産業技術総合研究機構 | 普通系コムギを定性的及び定量的に検出する方法 |
| KR101300845B1 (ko) * | 2010-12-06 | 2013-08-29 | 재단법인 제주테크노파크 | 백수오와 이엽우피소의 감별 방법 및 그 키트 |
| CN102146477B (zh) | 2011-04-06 | 2012-10-31 | 中华人民共和国珠海出入境检验检疫局 | 一种基于its基因的蔗扁蛾实时荧光pcr检测方法 |
| CN102222969B (zh) | 2011-06-13 | 2013-07-31 | 安徽理士电池技术有限公司 | 一种双电源电路系统 |
| CN102732513B (zh) | 2012-06-19 | 2014-01-15 | 中国医学科学院药用植物研究所 | 采用特异性引物pcr检测鼓槌石斛的方法 |
| CN104673930A (zh) | 2015-03-26 | 2015-06-03 | 湖南农业大学 | 一种罂粟的pcr检测方法 |
| CN105063203A (zh) | 2015-08-07 | 2015-11-18 | 重庆出入境检验检疫局检验检疫技术中心 | 一种用于人参实时荧光pcr检测的引物、探针及其检测方法 |
| CN105624291B (zh) | 2016-01-19 | 2019-01-11 | 中国食品药品检定研究院 | 检测样品中是否存在五加科植物成分及是否掺伪的方法 |
| CN105603107B (zh) | 2016-03-21 | 2019-06-21 | 中国中医科学院中药研究所 | 基于its序列位点的鉴别酸枣仁及其伪品的pcr方法及试剂盒 |
| CN106119394A (zh) | 2016-08-26 | 2016-11-16 | 刘明涛 | 一种世纬苣苔的核苷酸序列鉴定方法及核苷酸序列的应用 |
| CN107142329A (zh) | 2017-07-09 | 2017-09-08 | 中国农业科学院植物保护研究所 | 一种旱稻孢囊线虫lamp快速检测方法 |
| CN107653330A (zh) | 2017-11-02 | 2018-02-02 | 中国水稻研究所 | 一种快速鉴别飞虱种类的多重pcr试剂盒及其鉴别方法 |
| CN108642207B (zh) | 2018-05-08 | 2021-07-09 | 浙江师范大学 | 一种快速准确鉴定越橘属植物的检测方法 |
-
2020
- 2020-04-06 SI SI202030284T patent/SI3956455T1/sl unknown
- 2020-04-06 WO PCT/EP2020/059807 patent/WO2020212190A1/en not_active Ceased
- 2020-04-06 FI FIEP20719138.8T patent/FI3956455T3/fi active
- 2020-04-06 PT PT207191388T patent/PT3956455T/pt unknown
- 2020-04-06 PL PL20719138.8T patent/PL3956455T3/pl unknown
- 2020-04-06 KR KR1020217037173A patent/KR102926935B1/ko active Active
- 2020-04-06 EP EP20719138.8A patent/EP3956455B1/en active Active
- 2020-04-06 ES ES20719138T patent/ES2960514T3/es active Active
- 2020-04-06 HU HUE20719138A patent/HUE063576T2/hu unknown
- 2020-04-06 AU AU2020260318A patent/AU2020260318A1/en active Pending
- 2020-04-06 MX MX2021012714A patent/MX2021012714A/es unknown
- 2020-04-06 SG SG11202111379TA patent/SG11202111379TA/en unknown
- 2020-04-06 US US17/604,301 patent/US12571056B2/en active Active
- 2020-04-06 DK DK20719138.8T patent/DK3956455T5/da active
- 2020-04-06 JP JP2021560683A patent/JP7578608B2/ja active Active
- 2020-04-06 CN CN202080029311.4A patent/CN113692446B/zh active Active
- 2020-04-06 BR BR112021020571A patent/BR112021020571A2/pt unknown
- 2020-04-06 CA CA3136865A patent/CA3136865A1/en active Pending
-
2021
- 2021-10-12 IL IL287205A patent/IL287205B1/en unknown
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1823165A (zh) * | 2003-05-16 | 2006-08-23 | 家庭食品株式会社 | 食物或食物原材料中特定属的植物的定量pcr检测方法 |
Non-Patent Citations (2)
| Title |
|---|
| ACCESSION NO. AF382732，Vaccinium myrtillus internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence;无;《GenBank》;20020711;FEATURES, ORIGIN * |
| LAURA JAAKOLA ET AL.Novel approaches based on DNA barcoding and high-resolution melting of amplicons for authenticity analyses of berry species.《FOOD CHEMISTRY》.2010,第123卷(第2期),摘要，图1，表2，第2节 材料与方法. * |
Also Published As
| Publication number | Publication date |
|---|---|
| IL287205B1 (en) | 2026-01-01 |
| EP3956455B1 (en) | 2023-07-26 |
| US20220186326A1 (en) | 2022-06-16 |
| MX2021012714A (es) | 2021-11-12 |
| US12571056B2 (en) | 2026-03-10 |
| FI3956455T3 (fi) | 2023-10-11 |
| PT3956455T (pt) | 2023-10-26 |
| JP2022528973A (ja) | 2022-06-16 |
| ES2960514T3 (es) | 2024-03-05 |
| CA3136865A1 (en) | 2020-10-22 |
| JP7578608B2 (ja) | 2024-11-06 |
| BR112021020571A2 (pt) | 2022-01-25 |
| PL3956455T3 (pl) | 2024-01-03 |
| WO2020212190A1 (en) | 2020-10-22 |
| SI3956455T1 (sl) | 2023-11-30 |
| KR20210153663A (ko) | 2021-12-17 |
| DK3956455T5 (da) | 2024-08-05 |
| EP3956455A1 (en) | 2022-02-23 |
| DK3956455T3 (da) | 2023-10-09 |
| SG11202111379TA (en) | 2021-11-29 |
| HUE063576T2 (hu) | 2024-01-28 |
| KR102926935B1 (ko) | 2026-02-11 |
| IL287205A (en) | 2021-12-01 |
| AU2020260318A1 (en) | 2021-12-02 |
| CN113692446A (zh) | 2021-11-23 |
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