CN113684095B - Compound essential oil, preparation method thereof and product for improving learning and memory capacity - Google Patents

Compound essential oil, preparation method thereof and product for improving learning and memory capacity Download PDF

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CN113684095B
CN113684095B CN202110980021.4A CN202110980021A CN113684095B CN 113684095 B CN113684095 B CN 113684095B CN 202110980021 A CN202110980021 A CN 202110980021A CN 113684095 B CN113684095 B CN 113684095B
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eucalyptol
rosemary
peppermint
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CN113684095A (en
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安丽丽
吴亚妮
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An Lili
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Abstract

The application provides a compound essential oil, a preparation method thereof and a product for improving learning and memory capacity. Compound essential oil, comprising: 30-35wt% of eucalyptol rosemary essential oil, 20-25wt% of lemon essential oil, 10-15wt% of peppermint essential oil, 10-15wt% of borneol camphor essential oil, 10-15wt% of juniper berry essential oil, 5-7wt% of bergamot essential oil, 5-7wt% of clove essential oil and 5-7wt% of sandalwood essential oil. The preparation method of the compound essential oil comprises the following steps: pulverizing eucalyptol rosemary, lemon, peppermint, borneol camphor, juniper berry, bergamot, clove and sandalwood respectively, and extracting to obtain various essential oils; mixing the essential oils in proportion to obtain the compound essential oil. The product for improving learning and memory ability comprises the compound essential oil. The compound essential oil provided by the application can effectively improve learning and memory capacity.

Description

Compound essential oil, preparation method thereof and product for improving learning and memory capacity
Technical Field
The application relates to the field of daily necessities, in particular to compound essential oil, a preparation method thereof and a product for improving learning and memory capacity.
Background
The process of obtaining external information by humans and animals is known as learning, and the encoding, storage and reading of the obtained information is known as memory. Learning memory impairment refers to the problem of the ability to acquire, remember and reproduce information. Alzheimer's disease and vascular dementia among human diseases are mainly manifested by learning and memory disorders. And as the world population continues to age, the damage to humans by neurodegenerative diseases associated with aging, such as alzheimer's disease, becomes more apparent.
Essential oils, also known as volatile oils, refer to aromatic substances extracted from flowers, leaves, stems, fruits, or roots of plants by distillation, supercritical carbon dioxide extraction, etc. The essential oil mainly comprises natural compounds such as terpenes, aliphatic, aromatic and the like, has no chemical preservative, no essence pigment and high safety. Essential oils are small in chemical molecular weight and are mostly fat-soluble, and therefore readily cross the blood brain barrier. Essential oils are used to alleviate, prevent or treat a variety of diseases in the human body, and are increasingly used in the medical care field.
It is important to develop an essential oil with the ability of improving learning and memory.
Disclosure of Invention
The application aims to provide a compound essential oil, a preparation method thereof and a product for improving learning and memory capacity so as to solve the problems.
In order to achieve the above purpose, the present application adopts the following technical scheme:
a compound essential oil comprising: 30-35wt% of eucalyptol rosemary essential oil, 20-25wt% of lemon essential oil, 10-15wt% of peppermint essential oil, 10-15wt% of borneol camphor essential oil, 10-15wt% of juniper berry essential oil, 5-7wt% of bergamot essential oil, 5-7wt% of clove essential oil and 5-7wt% of sandalwood essential oil.
Preferably, the compound essential oil comprises: 30-34wt% of eucalyptol rosemary essential oil, 20-24wt% of lemon essential oil, 10-14wt% of peppermint essential oil, 10-14wt% of borneol camphor essential oil, 10-14wt% of juniper berry essential oil, 5-6wt% of bergamot essential oil, 5-6wt% of clove essential oil and 5-6wt% of sandalwood essential oil.
Preferably, the compound essential oil comprises: 30wt% of eucalyptol rosemary essential oil, 20wt% of lemon essential oil, 15wt% of peppermint essential oil, 10wt% of borneol camphor essential oil, 10wt% of juniper berry essential oil, 5wt% of bergamot essential oil, 5wt% of clove essential oil and 5wt% of sandalwood essential oil.
A preparation method of compound essential oil comprises the following steps:
pulverizing eucalyptol rosemary, lemon, peppermint, borneol camphor, juniper berry, bergamot, clove and sandalwood respectively, and extracting to obtain eucalyptol rosemary essential oil, lemon essential oil, peppermint essential oil, borneol camphor essential oil, juniper berry essential oil, bergamot essential oil, clove essential oil and sandalwood essential oil;
mixing eucalyptol rosemary essential oil, lemon essential oil, peppermint essential oil, borneol camphor essential oil, juniper berry essential oil, bergamot essential oil, clove essential oil and sandalwood essential oil according to a proportion to obtain the compound essential oil.
Preferably, the extracting comprises: the eucalyptol rosemary, peppermint, borneol camphor, juniper berry, clove and sandalwood are extracted by a steam distillation method, and the lemon and bergamot are extracted by a squeezing method.
Preferably, the steam distillation process is carried out at a temperature of from 100 ℃ to 120 ℃.
Preferably, the steam distillation method is carried out for 1h to 3h.
Preferably, in the steam distillation method, the steam pressure is 666.5-1333Pa.
The application also provides a product for improving learning and memory ability, which comprises the compound essential oil.
Compared with the prior art, the beneficial effects of this application include:
the compound essential oil provided by the application is prepared from plant essential oil extract containing natural active ingredients, namely eucalyptol rosemary essential oil, lemon essential oil, peppermint essential oil, borneol camphor essential oil, juniper berry essential oil, bergamot essential oil, clove essential oil and sandalwood essential oil, wherein D-borneol (borneol camphor essential oil) is core essential oil of the formula, is extracted from camphor tree varieties with Chinese characteristics, takes bicyclo monoterpene-D-borneol as a main component, and is a basis for improving the effect presentation of learning and memory capacity. The eucalyptol rosemary, peppermint and clove respectively take 1, 8-eucalyptol, menthol and eugenol as main components, have certain effect of refreshing, and the compounds belong to different categories of oxides, alcohols and phenols, the fragrance and the efficacy of the compounds need to be carefully matched, the efficacy of the borneol camphor is assisted and supplemented, and the effect is exerted while the excessive stimulation of cerebral nerves is avoided; the fragrance volatility of borneol camphor, eucalyptol rosemary, peppermint and clove is moderate, so that the fragrance and efficacy of the formula main body are maintained. The fragrance volatility of juniper berries, lemon and bergamot is high, and the fragrance is pleasant, so that the fragrance is integrated in the formula. The sandalwood has the functions of clear thinking and emotion stabilization, and the fragrance volatility is low, so that the sandalwood has the function and function of stabilizing the fragrance in the formula.
The formula is scientific in proportion, and no artificial compound is added. Animal behavioural and histopathological experimental researches show that the essential oil prepared by the method provided by the invention can improve the learning and memory behavior indexes of a learning and memory disorder model mouse in Morris water maze and a shuttle box, reduce the beta-amyloid deposition condition of a CA1 region of a brain hippocampus of the model mouse, and has the effect of improving learning and memory capacity. The essential oil provided by the application is convenient to use, and can be placed in a fragrance machine or directly smeared around the nasal cavity for fragrance expansion.
The preparation method of the compound essential oil provided by the application is simple in process, low in energy consumption, high in yield and excellent in quality.
The product for improving the learning and memory capacity can obtain the benefit of improving the learning and memory capacity by using the compound essential oil.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate certain embodiments of the present application and therefore should not be considered as limiting the scope of the present application.
FIG. 1 is a schematic illustration of escape latency in Morris water maze experiments;
FIG. 2 is a schematic diagram of the number of passes through the original platform in a space exploration experiment;
FIG. 3 is a schematic diagram of target quadrant residence time in a space exploration experiment;
FIG. 4 is a schematic diagram of the number of active avoidance times in a shuttle experiment;
FIG. 5 is a schematic of active avoidance latency in a shuttle experiment;
FIG. 6 is a schematic representation of mouse brain hippocampal CA1 region neuronal cells in an experiment for improving learning and memory impairment.
Detailed Description
The term as used herein:
"prepared from … …" is synonymous with "comprising". The terms "comprising," "including," "having," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, step, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, step, method, article, or apparatus.
The conjunction "consisting of … …" excludes any unspecified element, step or component. If used in a claim, such phrase will cause the claim to be closed, such that it does not include materials other than those described, except for conventional impurities associated therewith. When the phrase "consisting of … …" appears in a clause of the claim body, rather than immediately following the subject, it is limited to only the elements described in that clause; other elements are not excluded from the stated claims as a whole.
When an equivalent, concentration, or other value or parameter is expressed as a range, preferred range, or a range bounded by a list of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when ranges of "1 to 5" are disclosed, the described ranges should be construed to include ranges of "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a numerical range is described herein, unless otherwise indicated, the range is intended to include its endpoints and all integers and fractions within the range.
In these examples, the parts and percentages are by mass unless otherwise indicated.
"parts by mass" means a basic unit of measurement showing the mass ratio of a plurality of components, and 1 part may be any unit mass, for example, 1g may be expressed, 2.689g may be expressed, and the like. If we say that the mass part of the a component is a part and the mass part of the B component is B part, the ratio a of the mass of the a component to the mass of the B component is represented as: b. alternatively, the mass of the A component is aK, and the mass of the B component is bK (K is an arbitrary number and represents a multiple factor). It is not misunderstood that the sum of the parts by mass of all the components is not limited to 100 parts, unlike the parts by mass.
"and/or" is used to indicate that one or both of the illustrated cases may occur, e.g., a and/or B include (a and B) and (a or B).
A compound essential oil comprising: 30-35wt% of eucalyptol rosemary essential oil, 20-25wt% of lemon essential oil, 10-15wt% of peppermint essential oil, 10-15wt% of borneol camphor essential oil, 10-15wt% of juniper berry essential oil, 5-7wt% of bergamot essential oil, 5-7wt% of clove essential oil and 5-7wt% of sandalwood essential oil.
Preferably, the compound essential oil comprises: 30-34wt% of eucalyptol rosemary essential oil, 20-24wt% of lemon essential oil, 10-14wt% of peppermint essential oil, 10-14wt% of borneol camphor essential oil, 10-14wt% of juniper berry essential oil, 5-6wt% of bergamot essential oil, 5-6wt% of clove essential oil and 5-6wt% of sandalwood essential oil.
Preferably, the compound essential oil comprises: 30wt% of eucalyptol rosemary essential oil, 20wt% of lemon essential oil, 15wt% of peppermint essential oil, 10wt% of borneol camphor essential oil, 10wt% of juniper berry essential oil, 5wt% of bergamot essential oil, 5wt% of clove essential oil and 5wt% of sandalwood essential oil.
Optionally, in the compound essential oil, the content of eucalyptol rosemary essential oil may be any value between 30wt%, 31wt%, 32wt%, 33wt%, 34wt%, 35wt% or 30-35wt%, the content of lemon essential oil may be any value between 20wt%, 21wt%, 22wt%, 23wt%, 24wt%, 25wt% or 20-25wt%, the content of peppermint essential oil may be any value between 10wt%, 11wt%, 12wt%, 13wt%, 14wt%, 15wt% or 10-15wt%, the content of borneol camphor essential oil may be any value between 10wt%, 11wt%, 12wt%, 13wt%, 14wt%, 15wt%, or 10-15wt%, the content of juniper essential oil may be any value between 10wt%, 11wt%, 12wt%, 13wt%, 14wt%, 15wt%, or 10-15wt%, the content of bergamot essential oil may be any value between 5wt%, 6wt%, 7wt%, or any value between 5wt%, 7wt%, or 7wt% or any value between 7wt%, 7wt% or 7wt% of bergamot essential oil may be any value between 7wt%, or 7wt% or any value between 7wt% or 7wt% of essential oil.
A preparation method of compound essential oil comprises the following steps:
pulverizing eucalyptol rosemary, lemon, peppermint, borneol camphor, juniper berry, bergamot, clove and sandalwood respectively, and extracting to obtain eucalyptol rosemary essential oil, lemon essential oil, peppermint essential oil, borneol camphor essential oil, juniper berry essential oil, bergamot essential oil, clove essential oil and sandalwood essential oil;
mixing eucalyptol rosemary essential oil, lemon essential oil, peppermint essential oil, borneol camphor essential oil, juniper berry essential oil, bergamot essential oil, clove essential oil and sandalwood essential oil according to a proportion to obtain the compound essential oil.
Preferably, the extracting comprises: the eucalyptol rosemary, peppermint, borneol camphor, juniper berry, clove and sandalwood are extracted by a steam distillation method, and the lemon and bergamot are extracted by a squeezing method.
Preferably, the steam distillation process is carried out at a temperature of from 100 ℃ to 120 ℃.
Alternatively, the steam distillation method may be carried out at a temperature of 100 ℃, 110 ℃, 120 ℃, or any value between 100 ℃ and 120 ℃.
Preferably, the steam distillation method is carried out for 1h to 3h.
Alternatively, the steam distillation method may be performed for any value of 1h, 2h, 3h, or 1h-3h.
Preferably, in the steam distillation method, the steam pressure is 666.5-1333Pa.
Alternatively, in the steam distillation method, the steam pressure may be any value between 666.5Pa, 700Pa, 800Pa, 900Pa, 1000Pa, 1100Pa, 1200Pa, 1300Pa, 1333Pa, or 666.5-1333Pa.
The application also provides a product for improving learning and memory ability, which comprises the compound essential oil.
Embodiments of the present application will be described in detail below with reference to specific examples, but it will be understood by those skilled in the art that the following examples are only for illustration of the present application and should not be construed as limiting the scope of the present application. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
Example 1
The embodiment provides a compound essential oil, including: 30wt% of eucalyptol rosemary essential oil, 20wt% of lemon essential oil, 15wt% of peppermint essential oil, 10wt% of borneol camphor essential oil, 10wt% of juniper berry essential oil, 5wt% of bergamot essential oil, 5wt% of clove essential oil and 5wt% of sandalwood essential oil.
The embodiment also provides a method for preparing the compound essential oil, which comprises the following steps:
(1) Pulverizing eucalyptol rosemary, lemon, peppermint, borneol camphor, juniper berry, bergamot, clove and sandalwood respectively, extracting eucalyptol rosemary, peppermint, borneol camphor, juniper berry, clove and sandalwood by a steam distillation method, and extracting lemon and bergamot by a squeezing method to obtain essential oils of eucalyptol rosemary, lemon, peppermint, borneol camphor, juniper berry, bergamot, clove and sandalwood; the extraction temperature by steam distillation method is 100deg.C, and the extraction time is 3h.
(2) Mixing the eucalyptol rosemary, lemon, peppermint, borneol camphor, juniper berry, bergamot, clove and sandalwood essential oil obtained in the step (1) according to the proportion to obtain the compound essential oil.
Example 2
The embodiment provides a compound essential oil, including: 30wt% of eucalyptol rosemary essential oil, 25wt% of lemon essential oil, 10wt% of peppermint essential oil, 10wt% of borneol camphor essential oil, 10wt% of juniper berry essential oil, 5wt% of bergamot essential oil, 5wt% of clove essential oil and 5wt% of sandalwood essential oil.
Preparation method the procedure was the same as in example 1 except that the extraction temperature by steam distillation was 110℃and the extraction time was 2 hours.
Example 3
The embodiment provides a compound essential oil, including: 31wt% of eucalyptol rosemary essential oil, 21wt% of lemon essential oil, 10wt% of peppermint essential oil, 11wt% of borneol camphor essential oil, 10wt% of juniper berry essential oil, 6wt% of bergamot essential oil, 6wt% of clove essential oil and 5wt% of sandalwood essential oil.
Preparation method the procedure was the same as in example 1 except that the extraction temperature by steam distillation was 120℃and the extraction time was 1 h.
In order to better evaluate the efficacy of the compound essential oil provided by the application, the following experiment is specially carried out:
1. experiment for improving learning and memory ability
Animal behavioural experiments are carried out after the compound essential oil obtained in the example 1 is applied to mice to prove that the compound essential oil provided by the application has the efficacy of improving learning and memory capacity.
And setting a corresponding control group to perform a control experiment.
1. Experimental animal
24 SPF-class male 6-month-old APP/PS1 double-transgenic learning memory disorder model mice and 8 SPF-class male homogenic background normal mice are purchased from Kvines laboratory animal company, changzhou, license number SCXK (Su) 2016-0010. Feeding conditions: the light and shade are respectively 12 hours, the temperature is about 22 ℃, and the food can be eaten by drinking water freely.
2. Experimental grouping
24 model mice are randomly divided into a model group, a compound essential oil group and a positive group, and 8 normal mice are normal groups. The compound essential oil is sniffed by the compound essential oil group, and the rosemary essential oil is sniffed by the positive group (proved by a plurality of scholars to have the effect of improving learning and memory). The sniffing mode is to put the aromatherapy lamp filled with the 3% volume fraction essential oil water solution into a closed box, put the mice into the box after the essential oil volatilizes and diffuses, cover the box and sniff for 1h,2 times/day, and continuously for 21 days. Model and normal groups were untreated.
3. Experimental method
3.1 Morris Water maze experiment
The 12 th to 16 th days after the essential oil is sniffed are positioning navigation experiments, the water maze comprises quadrants I, II, III and IV, a platform is placed in a certain quadrant, and the platform is slightly lower than the water surface. Mice were placed in the pool from a quadrant and the time from entry into the water to successful stage was noted as escape latency. If the login is not successful in more than 120 seconds, the login is recorded as 120 seconds. 2 experiments were completed daily at different quadrant starting points. Day 17 is a space exploration experiment, the platform is removed, water is introduced from the starting point of a certain quadrant, and the number of times and the residence time of the mice crossing the original platform in the quadrant 120 seconds where the original platform is located are recorded.
3.2 shuttle experiments
The 20 th day after the essential oil is sniffed is a learning training stage, a mouse is put into one room, light and beeping sound are stimulated, the mouse is marked as an active avoidance if the mouse successfully escapes to the other room within 10 seconds, and the time used is an active avoidance latency period; if the mouse remains in the original chamber after 10 seconds, the administration of the electrical stimulus forces the mouse to escape to the other chamber. Each mouse was cycled 20 times. After 24 hours (day 21) the memory test phase was performed in the same manner as before.
4. Experimental results
4.1 Morris water maze test results
Positioning navigation experiment results:
as shown in figure 1 (note: compared to the normal group, * P<0.05， ** P<0.01; in comparison with the set of models, # P<0.05， ## P<0.01 The escape latency of the model group is obviously higher than that of the normal group and the escape latency of the compound essential oil group is obviously lower than that of the model group when the positioning navigation experiment starts on the 3 rd day, which indicates that the compound essential oil improves the learning and memory disorder of the model mice.
Space exploration experimental results:
as shown in fig. 2, the number of times of crossing the original platform is smaller than that of the normal group, and the compound essential oil group is more than that of the model group, so that the expected result is met, but no significant difference exists. As shown in fig. 3, the target quadrant residence time was less in the model group than in the normal group, more in the compound essential oil group than in the model group, consistent with the expected results, but without significant differences. And (3) injection: in comparison with the normal group, * P<0.05， ** P<0.01; in comparison with the set of models, # P<0.05， ## P<0.01。
4.2 results of shuttle experiments
As shown in fig. 4, in the aspect of the number of active avoidance, the compound essential oil greatly increased the number of active avoidance of the model mice in both the learning training and the memory testing phases. As shown in fig. 5, in the aspect of active avoidance latency, compound essential oil significantly reduced the active avoidance latency (P < 0.01) of the model mice during the learning training phase, and there was no significant difference between groups during the memory test phase.
4.3 knots
The compound essential oil improves the learning and memory behavior of mice with learning and memory impairment from the comprehensive view of 3 indexes of escape latency, the number of times of crossing the original platform and the target quadrant residence time of Morris water maze experiments, and 2 indexes of active avoidance times and active avoidance latency of shuttle experiments.
2. Experiment for improving learning and memory disorder
Histopathological experiments were performed after the compound essential oil obtained in example 1 was applied to mice to prove that the compound essential oil provided by the application has the efficacy of improving learning and memory disorders.
1. Experimental animal
24 SPF-class male 6-month-old APP/PS1 double-transgenic learning memory disorder model mice and 8 SPF-class male homogenic background normal mice are purchased from Kvines laboratory animal company, changzhou, license number SCXK (Su) 2016-0010. Feeding conditions: the light and shade are respectively 12 hours, the temperature is about 22 ℃, and the food can be eaten by drinking water freely.
2. Experimental grouping
24 model mice are randomly divided into a model group, a compound essential oil group and a positive group, and 8 normal mice are normal groups. The compound essential oil is sniffed by the compound essential oil group, and the rosemary essential oil is sniffed by the positive group (proved by a plurality of scholars to have the effect of improving learning and memory). The sniffing mode is to put the aromatherapy lamp filled with the 3% volume fraction essential oil water solution into a closed box, put the mice into the box after the essential oil volatilizes and diffuses, cover the box and sniff for 1h,2 times/day, and continuously for 21 days. Model and normal groups were untreated.
3. Experimental method
After the sniffing is completed. Taking mouse brain tissue, performing routine immunohistochemical experimental steps of embedding tissue, slicing, tissue fishing, dewaxing, antigen repairing, serum sealing, adding primary antibody, adding secondary antibody, adding SABC, adding color-developing agent, counterstaining, dehydrating and sealing, and observing the beta-amyloid precipitation condition of the CA1 region of the rat brain hippocampus under a microscope.
4. Experimental results
As shown in fig. 6, a. Is a normal group, B is a model group, C is a compound essential oil group, and D is a positive group; the arrow indicates the β -amyloid protein around the cell. No obvious beta-amyloid is observed around the neuronal cells in the CA1 zone of the hippocampus in the brains of the normal mice, the model group has brown obvious beta-amyloid, and the compound essential oil group and the positive group have light yellow beta-amyloid. The dyeing intensity shows that the compound essential oil group beta-amyloid protein precipitation is less than that of the model group, and the compound essential oil is used for improving brain tissue pathological conditions related to learning and memory of mice.
3. Brain wave experiment
Brain electricity is the synthesis of postsynaptic potential of brain neurons, and reflects brain electrical activity of brain tissue, functional state of brain and electrical parameters of brain neural activity. Common brain waves are classified into fast waves (the proportion of beta and gamma waves rises when wakefulness and memory and thinking are active), medium waves (the proportion of alpha waves rises when eyes are calm) and slow waves (the proportion of delta and theta waves rises when sleep). 40 volunteers were recruited, and brain wave change conditions were tested and analyzed before and after smelling the compound aroma for 15 minutes. The results are shown in table 1 below:
TABLE 1 brain wave variation
Test group Alpha wave (%) Beta wave (%) Delta wave (%) Theta wave (%)
Before smelling fragrance 44.15±4.24 23.37±3.17 15.66±2.18 16.82±1.84
After smelling the fragrance 43.63±5.71 25.16±3.22** 13.98±2.43* 16.23±2.36
As can be seen from table 2 above, the proportion of beta waves increases before and after smelling the fragrance of the compound essential oil, and the significant difference (p < 0.01) shows that brain waves representing excitation reaction and active thinking are excited; the decrease in the delta wave ratio indicates a decrease in the ratio of brain waves representing fatigue and shows a significant difference (p < 0.05). The compound essential oil fragrance is smelled, so that the thinking can be activated, the memory can be improved, and the brain fatigue can be relieved.
4. Sensory evaluation experiment
100 volunteers of different sexes and ages were recruited, and the aroma of 6 single essential oils and compound essential oils with refreshing effect, namely, eucalyptol rosemary essential oil, lemon essential oil, peppermint essential oil, borneol camphor essential oil, juniper berry essential oil and clove essential oil, was evaluated by an aroma preference evaluation table (scores of 0-9). Eucalyptol rosemary, peppermint, juniper berry, clove and lemon are common essential oils with refreshing effect, but have disadvantages. The fragrance of eucalyptol rosemary is less favored, and is not suitable for people with high blood pressure when used alone. The aroma of peppermint and clove is high in aroma ratio value, strong in irritation and obvious in dislike degree. The juniper berry essential oil has weak natural fragrance and general fragrance preference. Borneol camphor is a special camphor tree variety in China, is a new variety in essential oil resources, and is also a special essential oil in the formula.
The evaluation results are shown in the following table 2:
table 2 sensory evaluation results
As shown in table 2 above, in the evaluation of natural feeling of fragrance, refreshing feeling of fragrance and preference degree of fragrance, the compound essential oil is superior to other single essential oils, which indicates that the compound formula achieves good synergistic effect.
Comparative example 1
This comparative example provides a compound essential oil comprising: 31wt% of eucalyptol rosemary essential oil, 25wt% of lemon essential oil, 15wt% of peppermint essential oil, 12wt% of juniper berry essential oil, 6wt% of bergamot essential oil, 6wt% of clove essential oil and 5wt% of sandalwood essential oil.
The loss of borneol camphor essential oil can directly influence the stability of the proportion data of beta waves in brain wave experiments of volunteers, and the change does not show the difference, as shown in table 3, and the proportion of the beta waves is the most important index for representing the state of thinking activity and response learning and memory.
TABLE 3 brain wave variation
The compound essential oil provided by the application has the effect of improving learning and memory capacity. The compound essential oil is extracted from various natural plants, no artificial compound is added, and the safety is high. The essential oil has scientific formula, and the animal behaviours and the histopathological experiments prove that the effects can effectively improve the learning and memory ability. In addition, the essential oil has the characteristics of high stability and convenience in use, and has popularization and application values.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present application, and not for limiting the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present application.
Furthermore, those skilled in the art will appreciate that while some embodiments herein include some features but not others included in other embodiments, combinations of features of different embodiments are meant to be within the scope of the present application and form different embodiments. For example, in the claims below, any of the claimed embodiments may be used in any combination. The information disclosed in this background section is only for enhancement of understanding of the general background of the application and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.

Claims (9)

1. The compound essential oil for improving learning and memory capacity is characterized by comprising the following components: 30-35wt% of eucalyptol rosemary essential oil, 20-25wt% of lemon essential oil, 10-15wt% of peppermint essential oil, 10-15wt% of borneol camphor essential oil, 10-15wt% of juniper berry essential oil, 5-7wt% of bergamot essential oil, 5-7wt% of clove essential oil and 5-7wt% of sandalwood essential oil.
2. The compound essential oil for improving learning and memory ability according to claim 1, which is characterized by comprising the following components: 30-34wt% of eucalyptol rosemary essential oil, 20-24wt% of lemon essential oil, 10-14wt% of peppermint essential oil, 10-14wt% of borneol camphor essential oil, 10-14wt% of juniper berry essential oil, 5-6wt% of bergamot essential oil, 5-6wt% of clove essential oil and 5-6wt% of sandalwood essential oil.
3. The compound essential oil for improving learning and memory ability according to claim 1, which is characterized by comprising the following components: 30wt% of eucalyptol rosemary essential oil, 20wt% of lemon essential oil, 15wt% of peppermint essential oil, 10wt% of borneol camphor essential oil, 10wt% of juniper berry essential oil, 5wt% of bergamot essential oil, 5wt% of clove essential oil and 5wt% of sandalwood essential oil.
4. A method for preparing the compound essential oil for improving learning and memory ability as claimed in any one of claims 1 to 3, comprising:
pulverizing eucalyptol rosemary, lemon, peppermint, borneol camphor, juniper berry, bergamot, clove and sandalwood respectively, and extracting to obtain eucalyptol rosemary essential oil, lemon essential oil, peppermint essential oil, borneol camphor essential oil, juniper berry essential oil, bergamot essential oil, clove essential oil and sandalwood essential oil;
the compound essential oil for improving learning and memory is prepared by mixing eucalyptol rosemary essential oil, lemon essential oil, peppermint essential oil, borneol camphor essential oil, juniper berry essential oil, bergamot essential oil, clove essential oil and sandalwood essential oil according to a proportion.
5. The method of preparing according to claim 4, wherein the extracting comprises: the eucalyptol rosemary, peppermint, borneol camphor, juniper berry, clove and sandalwood are extracted by a steam distillation method, and the lemon and bergamot are extracted by a squeezing method.
6. The process according to claim 5, wherein the steam distillation is carried out at a temperature of 100℃to 120 ℃.
7. The method according to claim 6, wherein the steam distillation is carried out for a period of 1h to 3h.
8. The process according to claim 5, wherein the vapor pressure in the steam distillation is 666.5 to 1333Pa.
9. A product for improving learning and memory comprising the compound essential oil for improving learning and memory according to any one of claims 1 to 3.
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Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04149136A (en) * 1990-10-09 1992-05-22 Kureha Chem Ind Co Ltd Perfume composition and perfume product therefrom
RU2302256C1 (en) * 2006-04-27 2007-07-10 Вера Леонидовна Крылова Aromatic composition for normalizing psychoemotional state (variants)
CN102283891A (en) * 2011-05-06 2011-12-21 安徽丰乐香料有限责任公司 Medicament for accelerating post-anesthesia brain refreshing resuscitation and improving cognitive function of brain through olfactory pathway via nose
CN104449406A (en) * 2014-10-22 2015-03-25 武汉百年御马房汽车用品制造有限公司 Essential oil automobile wax with functions of refreshing brain and restoring consciousness and preparation method of essential oil automobile wax
CN105342887A (en) * 2015-11-24 2016-02-24 金玛瑙香水（明光）有限公司 Peppermint perfume and preparation method thereof
CN107468758A (en) * 2016-09-08 2017-12-15 安徽香杰香精科技有限公司 One kind is refreshed oneself anti-flu compound essential oil and its application method
CN107653118A (en) * 2016-07-25 2018-02-02 夏安娜 A kind of lemon clean skin soap strengthened memory and loosen nerve
CN109985113A (en) * 2018-01-02 2019-07-09 李康 Borneol health care essential oil and preparation method thereof
CN110038054A (en) * 2019-05-13 2019-07-23 江西中医药大学 Active component improves sleep or antidepression or improves in failure of memory drug or food and applies in preparation in gardenia and branches and leaves fruit
CN110179695A (en) * 2019-06-27 2019-08-30 东莞怀朴植物精油有限公司 A kind of improvement memory compound essential oil and preparation method thereof
CN111195330A (en) * 2018-11-19 2020-05-26 上海茗想健康科技有限公司 Compound essential oil for refreshing and restoring consciousness as well as preparation method and application thereof
CN111249180A (en) * 2019-10-11 2020-06-09 深圳市越昌科技有限公司 Refreshing perfume and preparation method thereof

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04149136A (en) * 1990-10-09 1992-05-22 Kureha Chem Ind Co Ltd Perfume composition and perfume product therefrom
RU2302256C1 (en) * 2006-04-27 2007-07-10 Вера Леонидовна Крылова Aromatic composition for normalizing psychoemotional state (variants)
CN102283891A (en) * 2011-05-06 2011-12-21 安徽丰乐香料有限责任公司 Medicament for accelerating post-anesthesia brain refreshing resuscitation and improving cognitive function of brain through olfactory pathway via nose
CN104449406A (en) * 2014-10-22 2015-03-25 武汉百年御马房汽车用品制造有限公司 Essential oil automobile wax with functions of refreshing brain and restoring consciousness and preparation method of essential oil automobile wax
CN105342887A (en) * 2015-11-24 2016-02-24 金玛瑙香水（明光）有限公司 Peppermint perfume and preparation method thereof
CN107653118A (en) * 2016-07-25 2018-02-02 夏安娜 A kind of lemon clean skin soap strengthened memory and loosen nerve
CN107468758A (en) * 2016-09-08 2017-12-15 安徽香杰香精科技有限公司 One kind is refreshed oneself anti-flu compound essential oil and its application method
CN109985113A (en) * 2018-01-02 2019-07-09 李康 Borneol health care essential oil and preparation method thereof
CN111195330A (en) * 2018-11-19 2020-05-26 上海茗想健康科技有限公司 Compound essential oil for refreshing and restoring consciousness as well as preparation method and application thereof
CN110038054A (en) * 2019-05-13 2019-07-23 江西中医药大学 Active component improves sleep or antidepression or improves in failure of memory drug or food and applies in preparation in gardenia and branches and leaves fruit
CN110179695A (en) * 2019-06-27 2019-08-30 东莞怀朴植物精油有限公司 A kind of improvement memory compound essential oil and preparation method thereof
CN111249180A (en) * 2019-10-11 2020-06-09 深圳市越昌科技有限公司 Refreshing perfume and preparation method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
朱复融.亚健康自然疗法全书.广东旅游出版社,2007,第452页. *

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