CN113663227B - Full-wrapping mouse hind limb ultraviolet radiation fixer - Google Patents

Full-wrapping mouse hind limb ultraviolet radiation fixer Download PDF

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Publication number
CN113663227B
CN113663227B CN202111045371.8A CN202111045371A CN113663227B CN 113663227 B CN113663227 B CN 113663227B CN 202111045371 A CN202111045371 A CN 202111045371A CN 113663227 B CN113663227 B CN 113663227B
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slit
main body
inner cylinder
outer cylinder
fixing mechanism
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CN202111045371.8A
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CN113663227A (en
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刘天一
董栋
陈亮
王恒
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Huadong Hospital
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Huadong Hospital
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61NELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N5/00Radiation therapy
    • A61N5/06Radiation therapy using light
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D3/00Appliances for supporting or fettering animals for operative purposes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D3/00Appliances for supporting or fettering animals for operative purposes
    • A61D2003/006Appliances for supporting or fettering animals for operative purposes with leg or foot restraining means
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61NELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N5/00Radiation therapy
    • A61N5/06Radiation therapy using light
    • A61N2005/0658Radiation therapy using light characterised by the wavelength of light used
    • A61N2005/0661Radiation therapy using light characterised by the wavelength of light used ultraviolet

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Radiology & Medical Imaging (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pathology (AREA)
  • Animal Husbandry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Position Input By Displaying (AREA)

Abstract

The invention discloses a fully-wrapped ultraviolet radiation fixer for a hind limb of a mouse, which comprises an outer cylinder rotary shielding mechanism, an inner cylinder fixing mechanism and a push rod fixing mechanism. The invention realizes the full-wrapping mouse hindlimb ultraviolet radiation fixer by arranging the mutual coordination of the outer cylinder rotary shielding mechanism, the inner cylinder fixing mechanism and the push rod fixing mechanism, and has the following beneficial effects: the mouse fixer is fully wrapped, so that the influence of ultraviolet radiation on other parts is eliminated; the whole process of the instrument does not contact the head of the mouse, so that the risk of bite is avoided; the mice do not need to be anesthetized, so that the damage of repeated anesthesia to the mice is reduced; the technical scheme is efficient and safe, is simple and convenient to operate, provides convenience for development of related experiments, can complete bilateral hind limb radiation and arbitrary unilateral hind limb radiation, and can observe the state of a mouse in real time when the mouse is fixed due to the transparent strip.

Description

Full-wrapping mouse hind limb ultraviolet radiation fixer
Technical Field
The invention relates to the technical field of animal experiment instruments, in particular to a fully-wrapped ultraviolet radiation fixator for hind limbs of mice.
Background
The incidence of skin cancer has increased year by year, and uv exposure is currently widely considered to be closely related to skin cancer. The research of the occurrence and metastasis mechanisms of ultraviolet rays and various skin malignant tumors requires the establishment of a local ultraviolet radiation model of the hind limbs of mice. The following requirements are set up for the model: first, in order to study the local effect, it is necessary to satisfy the local irradiation condition, and the exposure of the other parts except the irradiated part should be reduced as much as possible. Secondly, to ensure that the dose of the received radiation is uniform and stable, the position and posture of the mice are required to be fixed. In addition, chronic radiation modeling requires daily exposure to a dose of ultraviolet radiation for several consecutive days. However, there is currently no operating instrument capable of satisfying the above conditions. The current common method is to anesthetize the mice or fix the mice on a non-special instrument, shade the mice with black cloth or paper sheets, paper boards, etc., and then erect ultraviolet lamps above the mice to radiate the mice. However, this method has a number of problems:
1. Some experimental modeling needs to be carried out continuously for a plurality of days, a certain dose of ultraviolet radiation is received every day, and the normal physiological state of the mice is seriously influenced by repeated anesthesia, so that the accuracy of experimental results is influenced;
2. the shielding by materials such as cloth, paper board and the like is unavoidable, and the situation that shielding is insufficient and other parts are exposed is avoided. Such as eyes, which may cause blindness of mice and influence experimental results;
3. If the traditional mode is directly bound and fixed without anesthesia, the risk of being bitten by the mice is increased;
4. the operation of using the non-special instrument as the temporary fixing frame is complex, and the time and the labor are consumed;
in view of the above problems, no effective solution has been proposed at present.
Disclosure of Invention
The invention aims to provide a fully-wrapped ultraviolet radiation fixator for a hind limb of a mouse, which aims to solve the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions: the ultraviolet radiation fixer for the fully-wrapped mice hind limbs comprises an outer cylinder rotary shielding mechanism, an inner cylinder fixing mechanism and a push rod fixing mechanism, wherein the inner cylinder fixing mechanism is arranged in the outer cylinder rotary shielding mechanism, and the push rod fixing mechanism is arranged in the inner cylinder fixing mechanism;
The outer barrel rotary shielding mechanism comprises an outer barrel main body, wherein the left side and the right side of the top of the front of the outer barrel main body are respectively provided with a second slit and a first slit, and the inner wall of the outer barrel main body is fixedly connected with an annular bulge;
the inner cylinder fixing mechanism comprises an inner cylinder body arranged in the outer cylinder body, a fourth slit and a third slit are respectively formed in the left side and the right side of the bottom of the front surface of the inner cylinder body, a through slit is formed in the rear side of the inner cylinder body, a first inclined fixing plate and a second inclined fixing plate are respectively and fixedly connected to the positions, corresponding to the third slit and the fourth slit, of the inner wall side surface of the inner cylinder body, and a first transparent strip and a second transparent strip are respectively and fixedly connected to the positions, corresponding to the two sides of the slit, of the inner cylinder body;
Push rod fixed establishment is including setting up the inside horizontal pole of inner tube main part, the horizontal pole top all is connected with the elastic arm with the left side of bottom, one side fixedly connected with saw tooth pad of horizontal pole is kept away from to the elastic arm, the right-hand member of horizontal pole is through the screw mounting funnel, the bleeder vent has been seted up to the side of funnel.
Preferably, the outer cylinder body is matched with the inner cylinder body, and the surface of the inner cylinder body is contacted with the inner wall of the outer cylinder body.
Preferably, the top end of the outer cylinder body is in an open state, and the bottom end of the outer cylinder body is in a closed state.
Preferably, the inner cylinder main body is of a hollow tubular structure, and the top end and the bottom of the inner cylinder main body are both in an open state.
Compared with the prior art, the invention has the following beneficial effects:
The invention realizes the full-wrapping mouse hindlimb ultraviolet radiation fixer by arranging the mutual coordination of the outer cylinder rotary shielding mechanism, the inner cylinder fixing mechanism and the push rod fixing mechanism, and has the following beneficial effects:
1. The mouse fixer is fully wrapped, so that the influence of ultraviolet radiation on other parts is eliminated;
2. the whole process of the instrument does not contact the head of the mouse, so that the risk of bite is avoided;
3. the mice do not need to be anesthetized, so that the damage of repeated anesthesia to the mice is reduced;
4. The technical scheme is efficient and safe, is simple and convenient to operate, and provides convenience for developing related experiments;
5. according to the technical scheme, both side hind limb radiation and any single side hind limb radiation can be completed, and in addition, the existence of the transparent strip enables the state of the mouse to be observed in real time when the mouse is fixed.
Drawings
FIG. 1 is a schematic structural view of a perspective view of an outer cylinder rotary shielding mechanism of the invention;
FIG. 2 is a schematic perspective view of the inner cylinder fixing mechanism of the present invention;
FIG. 3 is a schematic top view of the inner barrel fixing mechanism of the present invention;
FIG. 4 is a schematic diagram of a front view of a push rod fixing mechanism according to the present invention;
Fig. 5 is a schematic structural view of a front view of the base of the present invention.
In the figure: the device comprises a 1 outer cylinder rotary shielding mechanism, a 101 outer cylinder main body, a 102 first slit, a 103 second slit, a 104 annular bulge, a 2 inner cylinder fixing mechanism, a 201 inner cylinder main body, a 202 third slit, a 203 fourth slit, a 204 passing slit, a 205 first inclined fixing plate, a 206 second inclined fixing plate, a 207 first transparent strip, a 208 second transparent strip, a 3 push rod fixing mechanism, a 301 cross rod, a 302 elastic arm, a 303 saw tooth pad, 304 screws, a 305 funnel and 306 ventilation holes.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-5, a fully-wrapped ultraviolet radiation fixator for hind limbs of mice comprises an outer cylinder rotary shielding mechanism 1, an inner cylinder fixing mechanism 2 and a push rod fixing mechanism 3, wherein the inner cylinder fixing mechanism 2 is arranged in the outer cylinder rotary shielding mechanism 1, and the push rod fixing mechanism 3 is arranged in the inner cylinder fixing mechanism 2.
The outer barrel rotating shielding mechanism 1 comprises an outer barrel main body 101, wherein the top end of the outer barrel main body 101 is in an open state, the bottom end of the outer barrel main body 101 is in a closed state, a second slit 103 and a first slit 102 are respectively formed in the left side and the right side of the front top of the outer barrel main body 101, and an annular protrusion 104 is fixedly connected to the inner wall of the outer barrel main body 101.
The inner cylinder fixing mechanism 2 comprises an inner cylinder main body 201 arranged in the inner cylinder main body 101, the outer cylinder main body 101 is matched with the inner cylinder main body 201, the surface of the inner cylinder main body 201 is contacted with the inner wall of the outer cylinder main body 101, the inner cylinder main body 201 is of a hollow tubular structure, the top end and the bottom of the inner cylinder main body 201 are in an open state, a fourth slit 203 and a third slit 202 are respectively formed on the left side and the right side of the front bottom of the inner cylinder main body 201, a through slit 204 is formed on the rear side of the inner cylinder main body 201, a first inclined fixing plate 205 and a second inclined fixing plate 206 are respectively fixedly connected to the side surface of the inner wall of the inner cylinder main body 201 corresponding to the positions of the third slit 202 and the fourth slit 203, a first transparent strip 207 and a second transparent strip 208 are respectively fixedly connected to the positions of the inner cylinder main body 201 corresponding to the two sides of the through slits 204, the inner cylinder fixing mechanism 2 is completely matched with the outer cylinder rotary shielding mechanism 1, the outer cylinder rotary shielding mechanism 1 can be just sleeved outside the inner cylinder fixing mechanism 2 and can freely rotate, one end D of the inner cylinder fixing mechanism 2 can be blocked and cannot be pushed forward when being pushed to the annular protrusion 104 of the outer cylinder rotary shielding mechanism 1, the other end C is just coincident with one end A of the outer cylinder rotary shielding mechanism 1, in addition, the third slit 202 and the fourth slit 203 of the inner cylinder fixing mechanism 2 are completely matched with the first slit 102 and the second slit 103 of the outer cylinder rotary shielding mechanism 1, when the end D is pushed to the annular protrusion 104, the first slit 102 is combined with the third slit 202, the second slit 103 is combined with the fourth slit 203 to just form two round holes, when the first slit 102 is combined with the fourth slit 203, or the second slit 103 is combined with the third slit 202 to just form one round hole, the original round hole on the other side is closed due to shielding, the first transparent strip 207 and the second transparent strip 208 on the two sides of the inner cylinder fixing mechanism 2, which pass through the slit 204, can be completely shielded by the outer cylinder rotary shielding mechanism 1.
The push rod fixing mechanism 3 comprises a cross rod 301 arranged inside the inner barrel main body 201, elastic arms 302 are connected to the left sides of the top and the bottom of the cross rod 301, saw-tooth pads 303 are fixedly connected to one sides of the elastic arms 302 away from the cross rod 301, the right ends of the cross rod 301 are provided with funnels 305 through screws 304, air holes 306 are formed in the side faces of the funnels 305, the push rod fixing mechanism 3 is completely matched with the inner barrel fixing mechanism 2, when two elastic arms 302 are pinched, the push rod fixing mechanism 3 can freely slide in the inner barrel fixing mechanism 2, when the two elastic arms 302 are loosened, the two saw-tooth pads 303 prop against the inner side wall of the inner barrel main body 201 and cannot move, all devices except for a first transparent strip 207 and a second transparent strip 208 on the side wall of the inner barrel main body 201 are black, materials including but not limited by acrylic plates, metals, wooden materials and the like are used together, the whole fixing device is matched with a base, and the base is provided with a semi-cylindrical concave which accommodates a plurality of fully wrapped mouse hindlimb fixing devices, and the whole wrapped mouse limb fixing device is completely matched with the fully wrapped mouse limb fixing device.
When the mouse is used, if the two side hind limbs of the mouse are required to be radiated, the tail of the mouse is lifted, the mouse is pulled into the inner cylinder along the slit from the C end of the inner cylinder main body 201 until the C end of the inner cylinder main body 201 is clamped by the baffle, the other hand is pinched to the tweezers-shaped structure of the push rod fixing mechanism 3, the end of the funnel 305 is sent into the inner cylinder main body 201 along the A end of the outer cylinder main body 101 and pushed forward, meanwhile, the situation of the mouse is observed from the transparent layer, the mouse is pushed until the movement of the mouse is completely limited, at this time, the mouse is completely fixed, the two hind limbs of the mouse are placed in the slit third slit 202 and the slit fourth slit 203, the push rod is properly adjusted, the first slit 102 and the second slit 103 on the outer cylinder main body 101 are pushed forward after being involuted with the slit third slit 202 and the slit fourth slit 203 until the two rear limbs of the mouse only pass through the two slit holes, at this time, the rest parts of the mouse are covered by the two rear limbs, and the light-transmitting parts of the two cylinders are completely wrapped by each other, and the slit is completely wrapped.
If only the right hind limb of the mouse is required to be radiated, the tail of the mouse is lifted, the mouse is pulled into the inner cylinder main body 201 along the slit from the C end until the tail is clamped by the baffle, the other hand is pinched by the tweezers-shaped structure of the push rod fixing mechanism 3, the end of the funnel 305 is sent into the inner cylinder main body 201 along the end A of the outer cylinder main body and pushed forward, meanwhile, the condition of the mouse is observed from the transparent layer, the push rod fixing mechanism 3 is released after the movement of the mouse is completely limited, at the moment, the mouse is completely fixed, the right hind limb of the mouse is pulled out and placed in the fourth slit 203, the push rod is properly adjusted, if the spoke is matched with the first slit 102 of the outer cylinder main body 101 and the fourth slit 203 of the inner cylinder main body 201, the two cylinders of the mouse are pushed forward until the two cylinders of the slit are mutually shielded, only the right hind limb of the mouse is remained to pass out of the slit, at the moment, other parts of the mouse are covered by the right hind limb, and the two cylinders of the slit are completely wrapped.
Similarly, if only the left hind limb of the mouse is required to be radiated, the tail of the mouse is lifted, the mouse is pulled into the inner cylinder main body 201 along the slit from the C end until the tail is clamped by the baffle plate, the other hand is pinched by the tweezers-shaped structure of the push rod fixing mechanism 3, the funnel 305 end is sent into the inner cylinder along the A end of the outer cylinder main body 101 and pushed forward, meanwhile, the situation of the mouse is observed from the transparent layer, and the mouse is pushed until the movement of the mouse is completely limited, so that the push rod fixing device is released. At this time, the right hind limb of the mouse is completely fixed, the right hind limb of the mouse is pulled out to be placed in the third slit 202, the push rod is properly adjusted, if the spoke is used for pushing the second slit 103 of the outer cylinder main body 101 and the third slit 202 of the inner cylinder main body 201 forwards after being matched, until the two slits are mutually shielded, only the right hind limb of the mouse is left to pass through the slit hole, at the moment, other parts of the mouse except the right hind limb are covered by wrapping, and the light-transmitting parts of the slits of the two cylinders are mutually shielded, so that the complete wrapping is achieved.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (1)

1. The utility model provides a full parcel nature mouse hindlimb ultraviolet radiation fixer, includes urceolus rotation shielding mechanism (1), inner tube fixed establishment (2) and push rod fixed establishment (3), its characterized in that: the inner cylinder fixing mechanism (2) is arranged in the outer cylinder rotary shielding mechanism (1), and the push rod fixing mechanism (3) is arranged in the inner cylinder fixing mechanism (2);
The outer cylinder rotary shielding mechanism (1) comprises an outer cylinder main body (101), wherein the top end of the outer cylinder main body (101) is in an opening state, the bottom end of the outer cylinder main body (101) is in a closed state, the left side and the right side of the front top of the outer cylinder main body (101) are respectively provided with a second slit (103) and a first slit (102), and an annular protrusion (104) is fixedly connected to the inner wall of the outer cylinder main body (101);
The inner cylinder fixing mechanism (2) comprises an inner cylinder main body (201) arranged in the outer cylinder main body (101), the outer cylinder main body (101) is matched with the inner cylinder main body (201), the surface of the inner cylinder main body (201) is contacted with the inner wall of the outer cylinder main body (101), the inner cylinder main body (201) is of a hollow tubular structure, the top end and the bottom of the inner cylinder main body (201) are in an open state, the left side and the right side of the front bottom of the inner cylinder main body (201) are respectively provided with a fourth crack (203) and a third crack (202), the rear side of the inner cylinder main body (201) is provided with a through crack (204), the inner wall side surface of the inner cylinder main body (201) is fixedly connected with a first inclined fixing plate (205) and a second inclined fixing plate (206) corresponding to the positions of a third slit (202) and a fourth slit (203) respectively, the inner cylinder main body (201) is fixedly connected with a first transparent strip (207) and a second transparent strip (208) corresponding to the positions of two sides of the slit (204) respectively, the inner cylinder fixing mechanism (2) is completely matched with the outer cylinder rotary shielding mechanism (1), the outer cylinder rotary shielding mechanism (1) can be just sleeved outside the inner cylinder fixing mechanism (2) and can freely rotate, when one end D of the inner cylinder fixing mechanism (2) is pushed to an annular bulge (104) of the outer cylinder rotary shielding mechanism (1), the inner cylinder main body can be blocked and cannot be pushed forwards, in addition, the third slit (202) and the fourth slit (203) of the inner cylinder fixing mechanism (2) are completely matched with the first slit (102) and the second slit (103) of the outer cylinder rotating shielding mechanism (1), when the end D is pushed to the annular bulge (104), the first slit (102) is combined with the third slit (202), the second slit (103) is combined with the fourth slit (203) to form two circular holes, when the first slit (102) is combined with the fourth slit (203) or the second slit (103) is combined with the third slit (202), a circular hole can be formed, the original circular hole on the other side is closed due to shielding, and the first transparent strip (207) and the second transparent strip (208) on the two sides of the inner cylinder fixing mechanism (2) can be completely shielded by the outer cylinder rotating shielding mechanism (1);
Push rod fixed establishment (3) are including setting up inside horizontal pole (301) of inner tube main part (201), the left side of horizontal pole (301) top and bottom all is connected with elastic arm (302), one side fixedly connected with saw-tooth pad (303) of horizontal pole (301) are kept away from to elastic arm (302), funnel (305) are installed through screw (304) to the right-hand member of horizontal pole (301), bleeder vent (306) have been seted up to the side of funnel (305), and push rod fixed establishment (3) and inner tube fixed establishment (2) are fit completely, and when holding between two elastic arms (302), push rod fixed establishment (3) can freely slide in inner tube fixed establishment (2), when unclamping two elastic arms (302), two saw-tooth pads (303) support inner tube main part (201) inside wall, then can not remove, except first transparent strip (207), second transparent strip (208) on inner tube main part (201) lateral wall, all other devices are black.
CN202111045371.8A 2021-09-07 2021-09-07 Full-wrapping mouse hind limb ultraviolet radiation fixer Active CN113663227B (en)

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Application Number Priority Date Filing Date Title
CN202111045371.8A CN113663227B (en) 2021-09-07 2021-09-07 Full-wrapping mouse hind limb ultraviolet radiation fixer

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Application Number Priority Date Filing Date Title
CN202111045371.8A CN113663227B (en) 2021-09-07 2021-09-07 Full-wrapping mouse hind limb ultraviolet radiation fixer

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CN113663227B true CN113663227B (en) 2024-05-31

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040078762A (en) * 2003-03-05 2004-09-13 한국원자력연구소 Seat assembly with support member for driver
JP2010035687A (en) * 2008-08-01 2010-02-18 Izumi-Cosmo Co Ltd Lancet fixing mechanism
CN202020800U (en) * 2011-03-31 2011-11-02 苏州大学 Drawer-type local irradiation device for tumor bearing mouse
KR20170111980A (en) * 2016-03-30 2017-10-12 주식회사 그린솔루스 A holder for experimental rat or mouse and gas chamber assembly having the same
KR101916390B1 (en) * 2017-07-11 2019-01-30 연세대학교 산학협력단 Stent holding apparatus for measuring dose perturbation caused from stent during x-ray and particle radiation therapies
CN109662799A (en) * 2018-12-14 2019-04-23 中国人民解放军军事科学院军事医学研究院 Rat fixator for thermometric in electromagnetic radiation environment
CN216395061U (en) * 2021-09-07 2022-04-29 华东医院 All-wrapping mouse hind limb ultraviolet radiation fixator

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040078762A (en) * 2003-03-05 2004-09-13 한국원자력연구소 Seat assembly with support member for driver
JP2010035687A (en) * 2008-08-01 2010-02-18 Izumi-Cosmo Co Ltd Lancet fixing mechanism
CN202020800U (en) * 2011-03-31 2011-11-02 苏州大学 Drawer-type local irradiation device for tumor bearing mouse
KR20170111980A (en) * 2016-03-30 2017-10-12 주식회사 그린솔루스 A holder for experimental rat or mouse and gas chamber assembly having the same
KR101916390B1 (en) * 2017-07-11 2019-01-30 연세대학교 산학협력단 Stent holding apparatus for measuring dose perturbation caused from stent during x-ray and particle radiation therapies
CN109662799A (en) * 2018-12-14 2019-04-23 中国人民解放军军事科学院军事医学研究院 Rat fixator for thermometric in electromagnetic radiation environment
CN216395061U (en) * 2021-09-07 2022-04-29 华东医院 All-wrapping mouse hind limb ultraviolet radiation fixator

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