CN113631564B - 具有硫代磷酸酯化部位的寡核苷酸的制造方法 - Google Patents

具有硫代磷酸酯化部位的寡核苷酸的制造方法 Download PDF

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Publication number
CN113631564B
CN113631564B CN202080025369.1A CN202080025369A CN113631564B CN 113631564 B CN113631564 B CN 113631564B CN 202080025369 A CN202080025369 A CN 202080025369A CN 113631564 B CN113631564 B CN 113631564B
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group
nucleic acid
oligonucleotide
protecting group
groups
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Chinese (zh)
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CN113631564A (zh
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市丸泰介
高桥大辅
平井邦博
山下健
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Ajinomoto Co Inc
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Ajinomoto Co Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Saccharide Compounds (AREA)
CN202080025369.1A 2019-03-28 2020-03-27 具有硫代磷酸酯化部位的寡核苷酸的制造方法 Active CN113631564B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019065158 2019-03-28
JP2019-065158 2019-03-28
PCT/JP2020/014308 WO2020196890A1 (ja) 2019-03-28 2020-03-27 ホスホロチオエート化部位を有するオリゴヌクレオチドの製造方法

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CN113631564A CN113631564A (zh) 2021-11-09
CN113631564B true CN113631564B (zh) 2025-02-25

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Country Link
US (1) US20220010348A1 (https=)
EP (1) EP3950698A4 (https=)
JP (2) JP7632276B2 (https=)
CN (1) CN113631564B (https=)
WO (1) WO2020196890A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115397831B (zh) * 2020-04-14 2026-02-24 住友化学株式会社 包含核酸寡聚物的组合物
EP4368627A4 (en) * 2021-07-06 2025-10-08 Sumitomo Chemical Co COMPOSITION CONTAINING A NUCLEIC ACID OLIGOMER
EP4559925A1 (en) * 2022-07-22 2025-05-28 Sumitomo Chemical Company, Limited Production method of oligonucleotide
WO2024083746A1 (en) 2022-10-17 2024-04-25 Bachem Holding Ag Method and composition for oligonucleotide synthesis
EP4695265A1 (en) 2023-10-16 2026-02-18 Bachem Holding AG Method and composition for oligonucleotide synthesis

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103476784A (zh) * 2011-05-17 2013-12-25 味之素株式会社 制备寡核苷酸的方法
CN104114569A (zh) * 2012-02-17 2014-10-22 味之素株式会社 碱基部保护寡核苷酸

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US7354907B2 (en) * 2003-02-07 2008-04-08 Idera Pharmaceuticals, Inc. Short immunomodulatory oligonucleotides
WO2005070859A1 (ja) 2004-01-27 2005-08-04 Takeshi Wada フルオラス担体およびそれを用いたオリゴヌクレオチド誘導体の製造方法
JP2007531794A (ja) * 2004-04-05 2007-11-08 アルニラム ファーマスーティカルズ インコーポレイテッド オリゴヌクレオチドの合成および精製に使用する方法および反応試薬
WO2011008682A1 (en) 2009-07-17 2011-01-20 Siemens Healthcare Diagnostics Inc Urinary trypsin inhibitors as diagnostic aid for interstitial cystitis
US9284344B2 (en) 2012-05-30 2016-03-15 Hokkaido System Science Co., Ltd. Oligonucleotide synthesis method using highly dispersible liquid-phase support
PL2872485T3 (pl) 2012-07-13 2021-05-31 Wave Life Sciences Ltd. Asymetryczna grupa pomocnicza
US10214555B2 (en) * 2012-11-14 2019-02-26 Takeda Pharmaceutical Company Limitjed Method for liquid-phase synthesis of nucleic acid
TWI729028B (zh) * 2015-11-17 2021-06-01 日商日產化學工業股份有限公司 寡核苷酸的製造方法
CN116063365A (zh) 2015-12-16 2023-05-05 味之素株式会社 寡核苷酸的制备方法以及核苷、核苷酸或寡核苷酸
TW201906855A (zh) 2017-05-02 2019-02-16 日商日產化學工業股份有限公司 寡核苷酸之製造方法
JP7140111B2 (ja) 2017-05-16 2022-09-21 日産化学株式会社 オリゴヌクレオチドの製造方法
JP7035430B2 (ja) 2017-09-29 2022-03-15 Dic株式会社 液晶組成物及びそれを使用した液晶表示素子

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103476784A (zh) * 2011-05-17 2013-12-25 味之素株式会社 制备寡核苷酸的方法
CN104114569A (zh) * 2012-02-17 2014-10-22 味之素株式会社 碱基部保护寡核苷酸

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Publication number Publication date
WO2020196890A1 (ja) 2020-10-01
JP7632276B2 (ja) 2025-02-19
JPWO2020196890A1 (https=) 2020-10-01
EP3950698A4 (en) 2023-01-25
US20220010348A1 (en) 2022-01-13
CN113631564A (zh) 2021-11-09
EP3950698A1 (en) 2022-02-09
JP2025028988A (ja) 2025-03-05

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