CN113620727A - Biogas slurry fertilizer and preparation method thereof - Google Patents
Biogas slurry fertilizer and preparation method thereof Download PDFInfo
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- CN113620727A CN113620727A CN202111046984.3A CN202111046984A CN113620727A CN 113620727 A CN113620727 A CN 113620727A CN 202111046984 A CN202111046984 A CN 202111046984A CN 113620727 A CN113620727 A CN 113620727A
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Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
- C05F3/04—Fertilisers from human or animal excrements, e.g. manure from human faecal masses
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/10—Addition or removal of substances other than water or air to or from the material during the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a biogas slurry fertilizer and a preparation method thereof, belonging to the technical field of agriculture and comprising the following steps: (1) mixing the biogas slurry with agricultural enzyme, and fermenting to obtain a mixture; (2) and (2) adding water into the mixture obtained in the step (1) to prepare the biogas slurry fertilizer. According to the invention, the agricultural enzyme is added to treat the biogas slurry, so that the phytotoxicity of the biogas slurry is reduced, the fertilizer effect is improved, the storage time of the biogas slurry is shortened, the discharge of irritant gas in the storage process is reduced, the environmental pollution is reduced, and the health of operators is protected.
Description
Technical Field
The invention belongs to the technical field of agriculture, and particularly relates to a biogas slurry fertilizer and a preparation method thereof.
Background
The biogas slurry is rich in nitrogen, phosphorus, potassium and a large amount of humus which are easy to absorb, and has great utilization potential. The commonly used biogas slurry treatment technologies at present comprise: a field returning method, an artificial wetland method, a membrane treatment method, and the like. The returning method is characterized in that the biogas slurry is statically placed in an open biogas slurry storage tank, mixed with water and applied to farmlands. The artificial wetland method purifies the biogas slurry by simulating the natural ecology of the wetland, but is easily influenced by seasons and environment, and the problem of accumulated silt is difficult to solve. The membrane treatment method is mainly used for separating solid from liquid of the biogas slurry in modes of ultrafiltration, nanofiltration, reverse osmosis, membrane distillation and the like, but because the components of the biogas slurry are relatively complex, the energy consumption of equipment is relatively high, the problem of membrane pollution is serious, and the operation cost is relatively high.
In recent years, research and development and popularization of a source separation water-saving toilet make resource utilization of human excrement possible. Compared with livestock and poultry manure produced in a farm, human manure collected through a toilet and a sewage pipe network contains various intestinal pathogenic bacteria, parasitic ova and viruses, is greatly different from the livestock and poultry manure in physical and chemical properties and microbial structures, and 70% of diseases in developing countries are related to biological infection of the human manure.
However, the current human excrement treatment process still applies the treatment flow of livestock and poultry excrement, an effective means for inhibiting pathogenic bacteria is lacked, the anaerobic fermentation temperature is generally 35 ℃, the removal rate of the pathogenic bacteria in the human excrement is low, and the biogas slurry is rich in ammonia-producing microorganisms, so that the accumulation of substances such as ammonia, organic acid and the like is easy to occur, and the threat to plant growth is formed. In the subsequent treatment process of the biogas slurry, an open aging tank is adopted, so that nutrients such as nitrogen, phosphorus and the like in the biogas slurry are greatly lost, the fertilizer effect of the biogas slurry is reduced, pathogenic bacteria in human excrement can be transmitted through air, the environmental pollution is caused, and the health of operators is threatened. In addition, insufficient decomposition of biogas slurry may cause ammonium ions, reducing substances, and the like to be contained in the biogas slurry. The problems result in phytotoxicity of the biogas slurry when applied as a fertilizer, resulting in crop reduction and environmental risks, which harm the health of operators.
Disclosure of Invention
The invention provides a biogas slurry fertilizer and a preparation method thereof, and the method reduces the phytotoxicity of biogas slurry, improves the fertilizer effect of the biogas slurry, shortens the storage time of the biogas slurry and reduces the discharge of irritant gas in the storage process by adding agricultural enzyme to treat the biogas slurry.
The invention provides a preparation method of biogas slurry fertilizer, which comprises the following steps:
(1) mixing the biogas slurry with agricultural enzyme, and fermenting to obtain a mixture;
(2) and (2) adding water into the mixture obtained in the step (1) to prepare the biogas slurry fertilizer.
Further, in the step (1), the biogas slurry is obtained by anaerobic fermentation of human excrement and urine.
Further, in the step (1), the biogas slurry is obtained by anaerobic fermentation of human excrement and at least one of straw, lawn grass and kitchen waste.
Further, the temperature of anaerobic fermentation is 35 ℃, and the hydraulic retention time of the anaerobic fermentation is 10-40 days; preferably, the anaerobic fermentation temperature is 35 ℃, and the hydraulic retention time of the anaerobic fermentation is 15-30 days.
Further, in the step (1), the volume ratio of the agricultural enzyme to the biogas slurry is 1: 100-1: 5.
Further, in the step (1), the volume ratio of the agricultural enzyme to the biogas slurry is 3: 100-1: 10.
Further, in the step (1), the fermentation temperature is 20-30 ℃, and the fermentation time is 20-30 days.
Further, in the step (1), the agricultural enzyme is prepared by mixing brown sugar, fruit and vegetable wastes and water according to the mass ratio of 1:3:10 and performing anaerobic fermentation at 30 ℃ for 90 days.
Further, in the step (2), the volume ratio of the mixture to the water is 1: 0.5-3.
The invention also provides the biogas slurry fertilizer prepared by any one of the methods.
The invention has the following advantages:
the agricultural enzyme is applied to the biogas slurry, so that the microbial community structure and the metabolic pathway of the microbial community are improved, the phytotoxicity of the biogas slurry is reduced, total ammonia nitrogen and free ammonia are reduced, the fertilizer effect is improved, the time required for storing and aging the biogas slurry can be shortened, the discharge of irritant gas in the storage process is reduced, the environmental pollution is reduced, and the health of operators is protected. The agricultural enzyme and the biogas slurry have synergistic effect, so that the fermentation time is shortened, the decomposition of organic matters is accelerated, the ammonia conversion is promoted, the inhibition of ammonia in the biogas slurry on the growth of plants is reduced, the nitrogen is preserved, the nitrification is promoted, and the growth of plants is promoted.
In addition, the method provided by the invention is simple to operate, short in fermentation time and simple in fermentation equipment, and can effectively reduce the biogas slurry treatment cost. The biogas slurry fertilizer prepared by the method can be used as a base fertilizer or an additional fertilizer, promotes the growth of crops and improves the quality of the crops.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a graph showing the change in Total Nitrogen (TN) during fermentation in examples of the present invention and comparative examples;
FIG. 2 is a graph showing the variation of Total Ammonia Nitrogen (TAN) in fermentation processes in examples of the present invention and comparative examples;
FIG. 3 is a graph showing the change of Free Ammonia (FAN) during fermentation in examples of the present invention and comparative examples;
FIG. 4 is a graph of the PCA analysis of microorganisms at the end of fermentation in examples of the present invention and comparative examples;
FIG. 5 is a graph showing the correlation and clustering of microorganisms and environmental factors at the end of fermentation in examples of the present invention and comparative examples;
FIG. 6 is a graph showing the effect of examples and comparative examples of the present invention on fresh weight of pakchoi;
FIG. 7 is a graph showing the effect of examples of the present invention and comparative examples on the dry weight of pakchoi;
FIG. 8 is a graph showing the effect of examples and comparative examples of the present invention on the leaf area of pakchoi.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The starting materials, reagents or apparatuses used in the following examples are conventionally commercially available or can be obtained by conventionally known methods, unless otherwise specified. The method is a conventional method, and all the methods are conventional methods unless otherwise specified.
An embodiment of the invention provides a preparation method of biogas slurry fertilizer, which comprises the following steps:
(1) mixing the biogas slurry with agricultural enzyme, and fermenting to obtain a mixture;
(2) and (2) adding water into the mixture obtained in the step (1) to prepare the biogas slurry fertilizer.
In the embodiment of the invention, the agricultural enzyme and the biogas slurry have synergistic effect to adjust the pH, shorten the fermentation time, accelerate the decomposition of organic matters, reduce the concentration of total ammonia nitrogen and free ammonia, contribute to reducing the inhibition of ammonia in the biogas slurry on the growth of plants, and reduce the discharge of irritant gas in the storage process.
The agricultural enzyme and the biogas slurry act together, a symbiotic network in the biogas slurry is inhibited, the succession direction of microorganisms is changed, dominant groups such as lactobacillus are enlarged by inhibiting pathogenic bacteria, microbial communities and metabolic pathways are improved, microbial activity is improved, physicochemical properties of the biogas slurry are improved, degradation of phytotoxic substances is accelerated, phytotoxicity is reduced, the value of a fertilizer is improved, plant growth is promoted, the time required by aging is shortened, discharge of irritant gas in the storage process is further reduced, environmental pollution is reduced, and the health of operators is protected.
In one embodiment of the invention, in the step (1), the biogas slurry is obtained by anaerobic fermentation of human excrement and urine.
In one embodiment of the invention, in the step (1), the biogas slurry is obtained by anaerobic fermentation of human excrement and at least one of straw, lawn grass and kitchen waste. The biogas slurry is not subjected to other treatment, the carbon content in anaerobic fermentation of the biogas slurry is increased by the lawn grass or the straws, the time required for storing the biogas slurry is shortened, the discharge of irritant gas in the storage process is reduced, and the solid content is adjusted.
Preferably, the biogas slurry is human excrement: kitchen waste: the turfgrass is obtained by anaerobic fermentation after being mixed according to the total solid ratio of 0-1:0.5-2: 1-2.
More preferably, the biogas slurry is human excrement: kitchen waste: the turfgrass is obtained by anaerobic fermentation after being mixed according to the total solid ratio of 0.335:1: 1.335.
In one embodiment of the invention, in the step (1), the anaerobic fermentation temperature of the biogas slurry is 35 ℃, and the hydraulic retention time of the anaerobic fermentation of the biogas slurry is 10-40 days.
Preferably, in the step (1), the temperature of anaerobic fermentation of biogas slurry is 35 ℃, and the hydraulic retention time of anaerobic fermentation of biogas slurry is 15-30 days. Under the condition of anaerobic fermentation of the biogas slurry, the methane yield is high, the fermentation is thorough, the gas production efficiency is high, and the biogas slurry is high in quality and has a promotion effect on plant growth. The biogas slurry obtained by the anaerobic fermentation method disclosed by the invention and the agricultural enzyme have a synergistic effect, so that the abundance of microorganisms inhibiting plant growth is effectively reduced, and the microbial succession direction of the biogas slurry is improved.
In an embodiment of the invention, in the step (1), the volume ratio of the agricultural enzyme to the biogas slurry is 1:100 to 1: 5.
Preferably, in the step (1), the volume ratio of the agricultural enzyme to the biogas slurry is 3: 100-1: 10. If the agricultural enzyme ratio is too high, the pH value is too low, and the fermentation process is inhibited; if the biogas slurry accounts for too high, enzyme microorganisms cannot be planted and the microbial structure of the biogas slurry cannot be optimized.
In one embodiment of the invention, in the step (1), the fermentation temperature is 20-30 ℃, and the fermentation time is 20-30 days.
In an embodiment of the present invention, in the step (1), the fermentation container is a light-proof container provided with a cover or sealed with a plastic film, and if the fermentation container is an airtight container, a pressure release valve needs to be provided.
In one embodiment of the invention, in the step (1), the agricultural enzyme is prepared by mixing brown sugar, fruit and vegetable wastes and water according to a mass ratio of 1:3:10 and performing anaerobic fermentation at 30 ℃ for 90 days. The microorganisms in the obtained agricultural enzyme have strong environment adaptability and are beneficial to the degradation of solid matters in the biogas slurry, and in addition, the agricultural enzyme adopts fruit and vegetable wastes as raw materials, so that the cost is greatly reduced compared with the cost of adopting chemical materials or fresh fruits and vegetables.
In one embodiment of the present invention, in the step (2), the volume ratio of the mixture to the water is 1: 0.5-3.
Preferably, in the step (2), the volume ratio of the mixture to the water is 1: 0.5-1.5.
More preferably, in step (2), the volume ratio of the mixture to water is 1:1.
The embodiment of the invention also provides the biogas slurry fertilizer prepared by any one of the preparation methods.
In one embodiment of the invention, the amount of biogas slurry fertilizer irrigated per month is 0.5-5 m per hectare according to crops and the growth period of the crops3。
The biogas slurry fertilizer prepared by the embodiment of the invention can be widely applied to crop planting. For example, the biogas slurry fertilizer can be used for planting pakchoi and the like, can reduce total ammonia nitrogen and free ammonia in biogas slurry, reduce the phytotoxicity generated by ammonia when the biogas slurry is applied, reduce the risk of environmental pollution caused by ammonia released when the biogas slurry is stored and applied, improve the germination accelerating effect of the fertilizer, increase the leaf area of pakchoi, improve the fresh weight content of pakchoi and improve the crop quality.
The present invention will be described in further detail with reference to examples, but the present invention is not limited to the following examples.
Example 1: preparation method of biogas slurry fertilizer
(1) Mixing the agricultural enzyme and the biogas slurry according to the volume ratio of 1:5 to prepare a mixture. Wherein the content of the first and second substances,
the agricultural enzyme is prepared by mixing brown sugar, fruit and vegetable wastes and water according to the mass ratio of 1:3:10, and fermenting at constant temperature of 30 ℃ for 90 days;
the biogas slurry is prepared by mixing human excrement: kitchen waste: mixing lawn grass at a total solid ratio of 0.335:1:1.335, Hydraulic Retention Time (HRT) for 30 days, and anaerobic fermenting at 35 deg.C.
(2) And (2) placing the mixture obtained in the step (1) in a lightproof container, sealing the container with a plastic film, and fermenting for 30 days at 25 ℃ to obtain a fermentation product.
(3) And (3) mixing the fermentation product obtained in the step (2) with clear water according to the volume ratio of 1:1 to prepare the biogas slurry fertilizer.
Example 2: the difference from example 1 is that in step (1), the agricultural enzyme and biogas slurry are mixed at a volume ratio of 1:10, and the volume difference from example 1 is eliminated by adding water.
Example 3: the difference from example 1 is that in step (1), the agricultural enzyme and biogas slurry are mixed at a volume ratio of 3:100, and the volume difference from example 1 is eliminated by adding water.
Example 4: the difference from example 1 is that in step (1), the agricultural enzyme and biogas slurry are mixed at a volume ratio of 1:100, and the volume difference from example 1 is eliminated by adding water.
Example 5: the difference from example 2 is that in step (1), the hydraulic retention time of biogas slurry in anaerobic fermentation is 25 days.
Example 6: the difference from example 2 is that in step (1), the hydraulic retention time of biogas slurry in anaerobic fermentation is 20 days.
Example 7: the difference from example 2 is that in step (1), the hydraulic retention time of biogas slurry in anaerobic fermentation is 15 days.
Comparative example 1: the difference from example 1 is that in step (1), no agricultural enzyme is added, and the volume difference from example 1 is eliminated by adding water.
Comparative example 2: the difference from comparative example 1 is that no agricultural enzyme was added in step (1) and no treatment in step (2) was performed, and the difference in volume from example 1 was eliminated by adding water.
Comparative example3: the difference from comparative example 2 is that clear water was used instead of biogas slurry for the experiment.
Comparative example 4: the difference from comparative example 1 is that in step (1), the hydraulic retention time of biogas slurry in anaerobic fermentation is 25 days.
Comparative example 5: the difference from comparative example 1 is that in step (1), the hydraulic retention time of biogas slurry in anaerobic fermentation is 20 days.
Comparative example 6: the difference from comparative example 1 is that in step (1), the hydraulic retention time of biogas slurry in anaerobic fermentation is 15 days.
Experimental example 1 Effect of different treatments on Nitrogen, microorganisms and environmental factors in biogas slurry
For ease of comparison, the specific composting experimental conditions of the above 7 examples and 6 comparative examples are shown in Table 1.
TABLE 1 biogas slurry treatment Experimental conditions
In the following, 7 groups of examples and 6 groups of examples are respectively examined on different physicochemical properties of the ratio, and the influence of the addition amount of agricultural enzyme and the treatment mode of biogas slurry on biogas slurry fertilizer is researched.
(1) Influence of different addition amounts of agricultural enzyme on nitrogen element in biogas slurry
As can be seen from FIG. 1, the total nitrogen content was higher in examples 1 (AJ-20%, 30d) and 2 (AJ-10%, 30d) at the end of the experiment, while in FIG. 2, the total ammonium nitrogen content was lower in examples 1 (AJ-20%, 30d) and 2 (AJ-10%, 30d) at the end of the experiment, and in FIG. 3, the free ammonia content was lower in examples 1 (AJ-20%, 30d), 2 (AJ-10%, 30d), 3 (AJ-3%, 30d) and 4 (AJ-1%, 30d) than in comparative examples 1 (AJ-0%, 30d) at the end of the experiment. The agricultural enzyme can promote nitrogen in the biogas slurry to be converted, so that the content of nitrogen elements in the biogas slurry is guaranteed, total ammonia nitrogen and free ammonia in the biogas slurry are reduced, and meanwhile, when the biogas slurry is applied, the phytotoxicity caused by ammonia and the risk of environmental pollution caused by ammonia release are reduced.
(2) Effect of different treatments on biogas slurry microbial communities and environmental factors
The sequencing results of comparative example 1 (AJ-0%, 30d), comparative example 2 (AJ-0%, biogas slurry stock solution, 30d) and example 2 (AJ-10%, 30d) at the end of fermentation were subjected to PCA analysis and correlation analysis of microbial environment factors, and the results are shown in FIG. 4 and FIG. 5, respectively.
In fig. 4, the data points of example 2 (AJ-10%, 30d) are far from those of comparative examples 1 (AJ-0%, 30d) and 2 (AJ-0%, biogas slurry stock solution, 30d), which indicates that the enzyme addition can change the direction of succession of microbial communities in the biogas slurry fermentation process. In addition, the distance between data points is smaller in example 2 (AJ-10%, 30d), which shows that the specificity of the biogas slurry microbial community is enhanced by adding the enzyme, and compared with the traditional treatment method, the quality of the biogas slurry fertilizer product obtained by the method is more stable.
FIG. 5 shows that the growth of most microorganisms inhibiting various physiological indexes of plants can be significantly inhibited by adding agricultural enzymes, which indicates that the phytotoxicity can be reduced by adding agricultural enzymes. The correlation clustering shows that the correlation level of the added agricultural enzyme and any plant physiological index is more close than any other physical and chemical index, and the addition of the agricultural enzyme can more effectively promote the growth of the plant compared with the change of a single physical and chemical index.
Experimental example 2 Effect of different treatments on phytotoxicity of biogas slurry
The experimental conditions were those of the above-mentioned 7 examples and 6 comparative examples, and are shown in Table 1. The germination index effect after the application of the 7 groups of examples and the 6 groups of groups in proportion is considered respectively, and the influence of the addition amount of the agricultural enzyme and the treatment mode of the biogas slurry on the biogas slurry fertilizer is researched.
Placing 25 full pakchoi seeds in a culture dish (diameter is 90mm) paved with filter paper, diluting the fermentation products obtained in the step (2) of the 7 groups of examples and 6 groups of group proportion by 100 times respectively to be used as culture solution, adding 3mL of the fermentation solution into the culture dish, culturing in a constant temperature incubator at 25 ℃, adding clear water once every 2 days in the culture process, and keeping the filter paper moist. During the culture process, the germination number of the seeds (the length of the embryo reaches the judgment standard that the seeds 1/2 are seeds germination) is counted every 24h, and the experiment lasts for 7 days. The Germination Index (GI) can be derived from formula (1):
GI ═ Σ (Gt/Dt) formula (1)
Wherein Gt is the number of sprouts of seeds on day t; and Dt is the germination days of the seeds.
TABLE 2 Germination index at the end of the experiment
Mean ± standard deviation (n ═ 3)
As can be seen from table 2, after the agricultural enzymes are added, the germination index of each example is higher than that of all comparative examples without the agricultural enzymes, which indicates that the addition of the agricultural enzymes is helpful to reduce the phytotoxicity of the biogas slurry and improve the germination accelerating effect. In the biogas slurry with the hydraulic retention time of 30d, the germination indexes of the example 2 (AJ-10%, 30d) and the example 3 (AJ-3%, 30d) are the highest, which shows that the addition of 3-10% of agricultural ferment is most helpful for improving the germination accelerating effect of the biogas slurry. GI of the hydraulically stayed biogas slurry treated by the enzyme is increased, which indicates that the hydraulic retention time is within 15-30, and the enzyme is added to improve the effect of the biogas slurry on promoting plant growth.
Experimental example 3 influence of different treatments on biogas slurry fertilizer effect
40g of the nutrient soil was put in a pot, 5 pakchoi seeds were sown at a depth of 0.5cm, and 25ml of the fertilizer obtained in the step (3) of examples 1 to 4 and comparative examples 1 to 3 was applied to the pakchoi seeds, respectively. The potted plant is cultivated in a greenhouse, one plantlet is cultivated in a two-leaf period, one plantlet is watered once every 2 days, and 20mL of plantlet is watered every time.
For comparison, the potting experimental conditions of the above examples 1 to 4 and comparative examples 1 to 3 are shown in Table 3.
TABLE 3 potted plant experimental conditions
Adopting a membership function method in fuzzy mathematics to comprehensively evaluate different physiological indexes and treatment matrixes of the Chinese cabbages in different treatments:
1) the form index membership value X (f) of the pakchoi plants under different treatments is obtained by the following formula:
X(f)＝(X-Xmin)/(Xmax-Xmin) (formula 2)
In the formula: x is a value measured in accordance with an index of one example or comparative example, XmaxThe maximum value, X, determined for this indexminIs the minimum value determined for the indicator.
2) And averaging the membership function values of different physiological indexes under each treatment to obtain a comprehensive evaluation coefficient.
TABLE 4 comprehensive evaluation of growth indexes of pakchoi under different treatments
n＝3,P<0.05
As can be seen from fig. 6 to 8 and table 4, the overall evaluation coefficient of example 2 (AJ-10%, 30d) is the highest, and compared with comparative example 1 (AJ-0%, 30d), the fresh weight is increased by 29.4%, the dry weight is increased by 15.6%, and the leaf area is increased by 50.6%. The comprehensive evaluation coefficients of examples 1 to 4 are all higher than those of comparative examples 1 to 3, which shows that 1 to 20 percent of the enzyme added amount has the promotion effect on the comprehensive condition of plant growth. Compared with the comparative example 2 (AJ-0%, biogas slurry stock solution, 30d), the fresh weight of the biogas slurry is increased by 29.9%, the dry weight of the biogas slurry is increased by 15.6%, and the leaf area of the biogas slurry is increased by 26.0% in example 3 (AJ-3%, 30d), which shows that the growth promoting effect of the pakchoi is the best when 3-10% of agricultural enzyme is added to treat the biogas slurry. Considering the comprehensive evaluation coefficient and the enzyme preparation cost, the enzyme addition amount is preferably 3-10%.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (10)
1. The preparation method of the biogas slurry fertilizer is characterized by comprising the following steps:
(1) mixing the biogas slurry with agricultural enzyme, and fermenting to obtain a mixture;
(2) and (2) adding water into the mixture obtained in the step (1) to prepare the biogas slurry fertilizer.
2. The preparation method according to claim 1, wherein in the step (1), the biogas slurry is obtained by anaerobic fermentation of human excrement and urine.
3. The preparation method according to claim 1, wherein in the step (1), the biogas slurry is obtained by anaerobic fermentation of at least one of human excrement and straw, lawn grass and kitchen waste.
4. The method according to any one of claims 1 to 3, wherein the anaerobic fermentation temperature is 35 ℃ and the hydraulic retention time of the anaerobic fermentation is 10 to 40 days; preferably, the anaerobic fermentation temperature is 35 ℃, and the hydraulic retention time of the anaerobic fermentation is 15-30 days.
5. The preparation method according to claim 1, wherein in the step (1), the volume ratio of the agricultural ferment to the biogas slurry is 1: 100-1: 5.
6. The preparation method according to claim 1, wherein in the step (1), the volume ratio of the agricultural ferment to the biogas slurry is 3: 100-1: 10.
7. The method according to claim 1, wherein in the step (1), the fermentation temperature is 20-30 ℃ and the fermentation time is 20-30 days.
8. The preparation method according to claim 1, wherein in the step (1), the agricultural enzyme is prepared by mixing brown sugar, fruit and vegetable wastes and water according to a mass ratio of 1:3:10 and performing anaerobic fermentation at 30 ℃ for 90 days.
9. The method according to claim 1, wherein in the step (2), the volume ratio of the mixture to water is 1: 0.5-3.
10. The biogas slurry fertilizer prepared by the preparation method of any one of claims 1 to 9.
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