CN113615837B - 一种延缓衰老的核苷酸益生菌胶囊 - Google Patents
一种延缓衰老的核苷酸益生菌胶囊 Download PDFInfo
- Publication number
- CN113615837B CN113615837B CN202110874599.1A CN202110874599A CN113615837B CN 113615837 B CN113615837 B CN 113615837B CN 202110874599 A CN202110874599 A CN 202110874599A CN 113615837 B CN113615837 B CN 113615837B
- Authority
- CN
- China
- Prior art keywords
- parts
- nucleotide
- probiotic
- capsule
- lactobacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000002775 capsule Substances 0.000 title claims abstract description 85
- 239000002773 nucleotide Substances 0.000 title claims abstract description 78
- 125000003729 nucleotide group Chemical group 0.000 title claims abstract description 77
- 239000006041 probiotic Substances 0.000 title claims abstract description 74
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 74
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 58
- 230000009758 senescence Effects 0.000 title claims abstract description 14
- 230000032683 aging Effects 0.000 claims abstract description 27
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 claims abstract description 17
- UDMBCSSLTHHNCD-UHFFFAOYSA-N Coenzym Q(11) Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1O UDMBCSSLTHHNCD-UHFFFAOYSA-N 0.000 claims abstract description 16
- PVBRXXAAPNGWGE-LGVAUZIVSA-L disodium 5'-guanylate Chemical compound [Na+].[Na+].C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP([O-])([O-])=O)[C@@H](O)[C@H]1O PVBRXXAAPNGWGE-LGVAUZIVSA-L 0.000 claims abstract description 16
- RAJMXAZJKUGYGW-POYBYMJQSA-N 2',3'-dideoxycytidine-5'-monophosphate Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP(O)(O)=O)CC1 RAJMXAZJKUGYGW-POYBYMJQSA-N 0.000 claims abstract description 14
- UPJKSWLLCONYMW-UHFFFAOYSA-N 5'-Adenosine monophosphate Natural products COc1cc(O)c(C(=O)C)c(OC2OC(COC3OC(C)C(O)C(O)C3O)C(O)C(O)C2O)c1 UPJKSWLLCONYMW-UHFFFAOYSA-N 0.000 claims abstract description 14
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 claims abstract description 13
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 13
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 13
- 244000199866 Lactobacillus casei Species 0.000 claims abstract description 13
- 235000013958 Lactobacillus casei Nutrition 0.000 claims abstract description 13
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 13
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 13
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 13
- 229940017800 lactobacillus casei Drugs 0.000 claims abstract description 13
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 13
- 235000013896 disodium guanylate Nutrition 0.000 claims abstract description 11
- 239000004193 disodium 5'-ribonucleotide Substances 0.000 claims abstract description 8
- IERHLVCPSMICTF-UHFFFAOYSA-N cytidine monophosphate Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(COP(O)(O)=O)O1 IERHLVCPSMICTF-UHFFFAOYSA-N 0.000 claims abstract description 5
- AEOBEOJCBAYXBA-UHFFFAOYSA-N A2P5P Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(O)=O)C(O)C1OP(O)(O)=O AEOBEOJCBAYXBA-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000000203 mixture Substances 0.000 claims description 14
- 239000011812 mixed powder Substances 0.000 claims description 13
- 240000002605 Lactobacillus helveticus Species 0.000 claims description 12
- 235000013967 Lactobacillus helveticus Nutrition 0.000 claims description 12
- 241000191996 Pediococcus pentosaceus Species 0.000 claims description 12
- 229940054346 lactobacillus helveticus Drugs 0.000 claims description 12
- 229920002774 Maltodextrin Polymers 0.000 claims description 10
- 239000005913 Maltodextrin Substances 0.000 claims description 10
- 229940035034 maltodextrin Drugs 0.000 claims description 10
- 241000894006 Bacteria Species 0.000 claims description 9
- DJJCXFVJDGTHFX-XVFCMESISA-L uridine 5'-monophosphate(2-) Chemical compound O1[C@H](COP([O-])([O-])=O)[C@@H](O)[C@@H](O)[C@@H]1N1C(=O)NC(=O)C=C1 DJJCXFVJDGTHFX-XVFCMESISA-L 0.000 claims description 9
- 108010073771 Soybean Proteins Proteins 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 6
- 159000000000 sodium salts Chemical group 0.000 claims description 6
- 235000019710 soybean protein Nutrition 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 5
- 229950006790 adenosine phosphate Drugs 0.000 claims description 3
- KURVIXMFFSNONZ-WFIJOQBCSA-L disodium;[(2r,3s,4r,5r)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound [Na+].[Na+].O[C@@H]1[C@H](O)[C@@H](COP([O-])([O-])=O)O[C@H]1N1C(=O)NC(=O)C=C1 KURVIXMFFSNONZ-WFIJOQBCSA-L 0.000 claims description 3
- IERHLVCPSMICTF-ZAKLUEHWSA-N cytidine-5'-monophosphate Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](COP(O)(O)=O)O1 IERHLVCPSMICTF-ZAKLUEHWSA-N 0.000 claims description 2
- 229940071440 soy protein isolate Drugs 0.000 claims description 2
- 238000005303 weighing Methods 0.000 claims description 2
- 235000013402 health food Nutrition 0.000 claims 1
- 239000000825 pharmaceutical preparation Substances 0.000 claims 1
- 229940127557 pharmaceutical product Drugs 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 22
- 230000036541 health Effects 0.000 abstract description 5
- 230000000968 intestinal effect Effects 0.000 abstract description 5
- DJJCXFVJDGTHFX-XVFCMESISA-N uridine 5'-monophosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N1C(=O)NC(=O)C=C1 DJJCXFVJDGTHFX-XVFCMESISA-N 0.000 abstract description 5
- 230000003712 anti-aging effect Effects 0.000 abstract description 3
- 239000003814 drug Substances 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 abstract description 3
- 230000036039 immunity Effects 0.000 abstract description 3
- 230000001105 regulatory effect Effects 0.000 abstract description 3
- 238000001727 in vivo Methods 0.000 abstract description 2
- 230000004792 oxidative damage Effects 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 13
- 239000000047 product Substances 0.000 description 12
- 241000699670 Mus sp. Species 0.000 description 11
- 239000000243 solution Substances 0.000 description 9
- -1 DPPH free radical Chemical class 0.000 description 8
- 150000003254 radicals Chemical class 0.000 description 7
- 241000282414 Homo sapiens Species 0.000 description 6
- 238000002835 absorbance Methods 0.000 description 6
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 210000001519 tissue Anatomy 0.000 description 6
- 102000019197 Superoxide Dismutase Human genes 0.000 description 5
- 108010012715 Superoxide dismutase Proteins 0.000 description 5
- 230000003078 antioxidant effect Effects 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 239000008367 deionised water Substances 0.000 description 5
- 229910021641 deionized water Inorganic materials 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 241000699666 Mus <mouse, genus> Species 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 4
- 210000005228 liver tissue Anatomy 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 231100000331 toxic Toxicity 0.000 description 4
- 230000002588 toxic effect Effects 0.000 description 4
- 239000012224 working solution Substances 0.000 description 4
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 235000005911 diet Nutrition 0.000 description 3
- 230000000378 dietary effect Effects 0.000 description 3
- 239000004198 disodium guanylate Substances 0.000 description 3
- 230000002708 enhancing effect Effects 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 239000012452 mother liquor Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 208000017667 Chronic Disease Diseases 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000001815 facial effect Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000003859 lipid peroxidation Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 150000002978 peroxides Chemical class 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000003860 sleep quality Effects 0.000 description 2
- 229940001941 soy protein Drugs 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- RQFCJASXJCIDSX-UHFFFAOYSA-N 14C-Guanosin-5'-monophosphat Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(COP(O)(O)=O)C(O)C1O RQFCJASXJCIDSX-UHFFFAOYSA-N 0.000 description 1
- RVBUGGBMJDPOST-UHFFFAOYSA-N 2-thiobarbituric acid Chemical compound O=C1CC(=O)NC(=S)N1 RVBUGGBMJDPOST-UHFFFAOYSA-N 0.000 description 1
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000006587 Glutathione peroxidase Human genes 0.000 description 1
- 108700016172 Glutathione peroxidases Proteins 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 230000002292 Radical scavenging effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000005056 compaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000037336 dry skin Effects 0.000 description 1
- 235000020774 essential nutrients Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000007760 free radical scavenging Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 210000004220 fundus oculi Anatomy 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000036449 good health Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 235000013928 guanylic acid Nutrition 0.000 description 1
- 230000003862 health status Effects 0.000 description 1
- 208000026278 immune system disease Diseases 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 229940118019 malondialdehyde Drugs 0.000 description 1
- 230000006993 memory improvement Effects 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 230000006996 mental state Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 235000006286 nutrient intake Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 230000019612 pigmentation Effects 0.000 description 1
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/13—Nucleic acids or derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
- A61K31/706—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
- A61K31/7064—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines
- A61K31/7068—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines having oxo groups directly attached to the pyrimidine ring, e.g. cytidine, cytidylic acid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
- A61K31/706—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
- A61K31/7064—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines
- A61K31/7068—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines having oxo groups directly attached to the pyrimidine ring, e.g. cytidine, cytidylic acid
- A61K31/7072—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines having oxo groups directly attached to the pyrimidine ring, e.g. cytidine, cytidylic acid having two oxo groups directly attached to the pyrimidine ring, e.g. uridine, uridylic acid, thymidine, zidovudine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
- A61K31/706—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
- A61K31/7064—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines
- A61K31/7076—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines containing purines, e.g. adenosine, adenylic acid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
- A61K31/706—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
- A61K31/7064—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines
- A61K31/7076—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines containing purines, e.g. adenosine, adenylic acid
- A61K31/708—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines containing purines, e.g. adenosine, adenylic acid having oxo groups directly attached to the purine ring system, e.g. guanosine, guanylic acid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/4816—Wall or shell material
- A61K9/4825—Proteins, e.g. gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/147—Helveticus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/41—Pediococcus
- A23V2400/427—Pentosaceus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biochemistry (AREA)
- Nutrition Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Toxicology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicinal Preparation (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
本发明公开一种延缓衰老的核苷酸益生菌胶囊,属于医药保健技术领域,本发明的核苷酸益生菌胶囊包括胶囊壳和内容物,内容物组分包括4种外源核苷酸和5种益生菌,外源核苷酸包括5’单磷酸胞苷(5’‑CMP)、5’单磷酸腺苷(5’‑AMP)、5’尿苷酸二钠(5’‑UMPNa2)和5’鸟苷酸二钠(5’‑GMPNa2),益生菌包括嗜酸乳杆菌、植物乳杆菌、干酪乳杆菌等益生菌,益生菌含量均大于1×107CFU/g。本发明的延缓衰老的核苷酸益生菌胶囊,能够清除体内自由基,抑制自由基对机体的氧化损伤,延长细胞寿命,具有延缓衰老、延年益寿、提高免疫力、调节肠道菌群等多种保健功效,并且携带服用方便,是适合中老年人的保健食品。
Description
技术领域
本发明属于医药保健技术领域,具体涉及一种延缓衰老的核苷酸益生菌胶囊。
背景技术
衰老是指人类在发育成熟以后,随时间的增加,机体出现组织结构变化,机能衰退、适应性和抵抗力减退,趋于死亡的过程。衰老作为复杂生命现象的一种变化,是自发的必然的过程。随着人们生活水平的提高,中国即将步入老龄化社会,延缓衰老、健康长寿是很多人尤其是女性追求的目标。追求延年益寿是人类几千年的梦想。如今,预防和延缓衰老已成为当前研究的热点。研究发现,衰老是多种因素综合作用的结果,与遗传基因、自由基、免疫功能紊乱等因素有关。虽然衰老是每个人生命活动中不可抗拒的自然规律,但是采用良好的保健措施,服用延缓衰老的产品,可有效地延缓衰老,推迟生老病死的时间,提高生活质量,让人类在现有的条件下实现最大极限的长寿。
核苷酸是细胞的重要组成成分,是DNA和RNA的前体,在细胞代谢、蛋白质合成和细胞分裂等过程中起着重要的作用。核苷酸在一些特定条件下,如疾病、快速生长发育、营养摄入受限以及内源性合成和表达受阻时,核苷酸成为机体的“必需营养素”。因此,核苷酸可作为实现延缓衰老、益寿延年的潜在物质。
机体衰老的同时会伴随多种慢性疾病的产生,而这些慢性疾病很多都与肠道菌群失调相关。因此,肠道菌群也是影响机体衰老的一个重要指标。益生菌是通过定殖在人体内,对人体健康有重要作用的活性微生物。研究发现,益生菌在调节肠道菌群平衡、促进营养吸收、保持肠道健康等方面具有重要的功效。
目前市场上现有的用于延缓衰老的产品很多,但是这些产品作用低微,见效迟缓,效果较差。因此,研究开发一种成分安全有效,无毒副作用、能够高效延缓机体老化的产品成为当前亟待研究的重要课题。
发明内容
本发明针对上述问题,本发明提供一种延缓衰老的核苷酸益生菌胶囊,其包含4种外源核苷酸和5种益生菌,成分安全有效,无毒副作用,具有增强免疫力、延缓机体老化、增强细胞生命力的功效。
本发明为实现上述目的,通过以下技术方案实现:
一种延缓衰老的核苷酸益生菌胶囊,包括椭圆形胶囊和内容物,所述内容物包括4种外源5’核苷酸或者其钠盐形式的混合物和5种益生菌,重量配比为:5’核苷酸混合物45~75份,益生菌20~30份。
优选的是,所述内容物中4种外源5’核苷酸的重量百分比分别为:CMP 15~45%、AMP15~55%、UMP 6~30%、GMP 14~35%。
优选的是,所述内容物益生菌中各菌种质量百分比为:嗜酸乳杆菌17~32%、植物乳杆菌17~32%、干酪乳杆菌17~32%、瑞士乳杆菌10~23%、戊糖片球菌7~19%。
优选的是,所述内容物的重量配比为:5’单磷酸胞苷(5’-CMP)10~20份,5’单磷酸腺苷(5’-AMP)20~30份,5’尿苷酸二钠(5’-UMPNa2)5~10份,5’鸟苷酸二钠(5’-GMPNa2)10~15份,嗜酸乳杆菌5~7份,植物乳杆菌5~7份,干酪乳杆菌5~7份,瑞士乳杆菌3~5份,戊糖片球菌2~4份。
优选的是,5’单磷酸胞苷10份,5’单磷酸腺苷20份,5’尿苷酸二钠5份,5’鸟苷酸二钠10份,嗜酸乳杆菌5份,植物乳杆菌5份,干酪乳杆菌5份,瑞士乳杆菌3份,戊糖片球菌2份。
优选的是,5’单磷酸胞苷15份,5’单磷酸腺苷25份,5’尿苷酸二钠8份,5’鸟苷酸二钠10份,嗜酸乳杆菌6份,植物乳杆菌6份,干酪乳杆菌6份,瑞士乳杆菌4份,戊糖片球菌3份。
优选的是,5’单磷酸胞苷20份,5’单磷酸腺苷30份,5’尿苷酸二钠10份,5’鸟苷酸二钠15份,嗜酸乳杆菌7份,植物乳杆菌7份,干酪乳杆菌7份,瑞士乳杆菌5份,戊糖片球菌4份。
优选的是,所述益生菌每种菌种的活菌含量均大于1×107CFU/g。
优选的是,胶囊的壁材为麦芽糊精和大豆分离蛋白。
优选的是,所述麦芽糊精和大豆分离蛋白的重量比为1:1~1:2。。
优选的是,所述麦芽糊精和大豆分离蛋白的重量比为1:1.5~1:2。
优选的是,所述核苷酸包括天然核苷酸和人工合成核苷酸。
优选的是,所述益生菌为益生菌活菌冻干粉。
本发明另一方面提供上述核苷酸益生菌胶囊的制备方法,按配比称量上述内容物,混合均匀,获得混合粉,将混合粉装入空心胶囊中,即得核苷酸益生菌胶囊。
本发明另一方面提供上述核苷酸益生菌胶囊在制备延缓衰老的保健食品和/或药品中的应用。
本发明相对于现有技术具有的有益效果如下:
1.本发明是以膳食核苷酸和益生菌为原料,成分安全有效,无毒副作用,二者配合服用,协同作用,能够清除体内自由基,抑制自由基对机体的氧化损伤,延长细胞寿命,具有延缓衰老、延年益寿、提高免疫力、调节肠道菌群等多种保健功效,并且携带服用方便,是适合中老年人的保健食品。
2.本发明的核苷酸益生菌胶囊的制备工序简单易操作,稳定性好,长期服用有益于身体健康。
具体实施方式
下面通过实施例的方式进一步说明本发明,但并不因此将本发明限制在所述的实施例范围之中。下述实施例中,如无特殊说明,所使用的实验方法均为常规方法,所用材料、试剂等均可从生物或化学公司购买。
本发明的延缓衰老的核苷酸益生菌胶囊,包括椭圆形胶囊皮和内容物,胶囊皮的成分包括麦芽糊精和大豆分离蛋白;内容物包含4种膳食核苷酸和多种益生菌。
实施例1
一种延缓衰老的核苷酸益生菌胶囊,包括椭圆形胶囊皮和内容物,胶囊壁材为麦芽糊精、大豆分离蛋白,二者重量比为1:1,内容物组分包括4种5’单核苷酸或者其钠盐形式的混合物和5种益生菌活菌冻干粉,按重量份配比为:5’单磷酸胞苷10份,5’单磷酸腺苷20份,5’尿苷酸二钠5份,5’鸟苷酸二钠10份,嗜酸乳杆菌5份,植物乳杆菌5份,干酪乳杆菌5份,瑞士乳杆菌3份,戊糖片球菌2份。具体制备方法为将上述内容物按配比混合均匀,获得混合粉,将混合粉装入空心胶囊中,每粒胶囊装入0.2g,即得核苷酸益生菌胶囊成品。
实施例2
一种延缓衰老的核苷酸益生菌胶囊,包括椭圆形胶囊皮和内容物,胶囊壁材为麦芽糊精、大豆分离蛋白,二者重量比为1:1.5,内容物组分包括4种5’单核苷酸或者其钠盐形式的混合物和5种益生菌活菌冻干粉,按重量份配比为:5’单磷酸胞苷15份,5’单磷酸腺苷25份,5’尿苷酸二钠8份,5’鸟苷酸二钠10份,嗜酸乳杆菌6份,植物乳杆菌6份,干酪乳杆菌6份,瑞士乳杆菌4份,戊糖片球菌3份。具体制备方法为将上述内容物按配比混合均匀,获得混合粉,将混合粉装入空心胶囊中,每粒胶囊装入0.2g,即得核苷酸益生菌胶囊成品。
实施例3
一种延缓衰老的核苷酸益生菌胶囊,包括椭圆形胶囊皮和内容物,胶囊壁材为麦芽糊精、大豆分离蛋白,二者重量比为1:2,内容物组分包括4种5’单核苷酸或者其钠盐形式的混合物和5种益生菌活菌冻干粉,按重量份配比为:5’单磷酸胞苷20份,5’单磷酸腺苷30份,5’尿苷酸二钠10份,5’鸟苷酸二钠15份,嗜酸乳杆菌7份,植物乳杆菌7份,干酪乳杆菌7份,瑞士乳杆菌5份,戊糖片球菌4份。具体制备方法为将上述内容物按配比混合均匀,获得混合粉,将混合粉装入空心胶囊中,每粒胶囊装入0.2g,即得核苷酸益生菌胶囊成品。
实施例4
为了验证本发明的核苷酸益生菌胶囊对延缓衰老的作用,本实验首先通过考察复配核苷酸的抗氧化性能。
按照实施例1中的核苷酸益生菌胶囊中核苷酸的配比配制5’单磷酸胞苷:5’单磷酸腺苷:5’尿苷酸二钠:5’鸟苷酸二钠重量比为10:20:5:10的混合核苷酸,取0.1g溶于100mL去离子水,制的混合核苷酸母液,进行DPPH自由基清除率的测定和ABTS自由基清除率的测定。
1.DPPH自由基清除率的测定:
将母液分别稀释100,50,25,10,1倍制备成浓度为10μg/mL,20μg/mL,40μg/mL,100μg/mL,500μg/mL的梯度稀释液。
分别取上述浓度150μL样品和150μL浓度为60mg/L的DPPH溶液(无水乙醇稀释)置于96孔板中,混合均匀,30℃避光保存,30min后使用酶标仪测量519nm下吸光度值。DPPH自由基清除率公式如下:
式中,A空白为150μL去离子水和150μL DPPH溶液的吸光度,A样品为150μL样品和150μL DPPH溶液的吸光度;IC50为DPPH自由基清除率达到50%时样品的浓度。
2.ABTS自由基清除率的测定:
(1)将母液分别稀释100,50,25,10,1倍制备成浓度为10μg/mL,20μg/mL,40μg/mL,100μg/mL,500μg/mL的梯度稀释液。
(2)将7mmol/L的ABTS溶液和140mmol/L的过硫酸钾溶液以62.5:1的比例混合,室温避光静置过夜,形成ABTS储备液,使用前用去离子水稀释成5mmol/L的ABTS工作液。
(3)分别取0.15mL上述稀释液样品与2.85mL ABTS工作液混合,30℃下静置8min,以去离子水为空白在734nm下测定吸光度。
ABTS自由基清除率公式如下:
式中,A空白为0.15mL去离子水和2.85mL ABTS工作液的吸光度,A样品为0.15mL样品和2.85mL ABTS工作液的吸光度。IC50为ABTS自由基清除率达到50%时样品的浓度。
参考上述同样的检测方法检测实施例2中的核苷酸益生菌胶囊中核苷酸的配比配制5’单磷酸胞苷:5’单磷酸腺苷:5’尿苷酸二钠:5’鸟苷酸二钠重量比为15:25:8:10的混合核苷酸以及实施例3中的核苷酸益生菌胶囊中核苷酸的配比配制5’单磷酸胞苷:5’单磷酸腺苷:5’尿苷酸二钠:5’鸟苷酸二钠重量比为20:30:10:15的混合核苷酸的抗氧化性能,同时以配制5’单磷酸胞苷:5’单磷酸腺苷:5’尿苷酸二钠:5’鸟苷酸二钠重量比为1:1:1:1的混合核苷酸作为对照实验,实验数据以均值±标准偏差表示,实验结果如表1所示。
表1.混合核苷酸的抗氧化性能
结果表明,与对照组相比,实施例1-3的核苷酸混合物均具有较高的抗氧化性能,能够更有效的清除机体的自由基,从而高效延缓机体的老化和增强细胞生命力。
实施例5
机体代谢会产生大量的氧化物自由基,破坏细胞功能,导致器官老化,从而导致机体的衰老。超氧化物歧化酶(SOD)可以清除对机体有害的超氧阴离子自由基。谷胱甘肽过氧化物酶(GSH-Px)是机体内广泛存在的一种重要的过氧化物分解酶。可以使有毒的过氧化物还原成无毒的羟基化合物,从而保护细胞膜的结构及功能不受过氧化物的干扰及损害。因此,SOD和GSH-Px活性可以反映出组织的抗氧化能力,是评价机体衰老的重要指标。MDA是脂质的过氧化产物,与机体内自由基的产生情况和机体组织细胞的脂质过氧化程度密切相关,可反映出机体组织的氧化应激的损伤状态,也作为评价衰老的重要指标之一。为进一步地验证本发明的核苷酸益生菌胶囊对延缓衰老的作用,对实施例1~3中制备的的核苷酸益生菌胶囊进行动物实验,本发明采用快速老化倾向SAMP-8小鼠,测试本发明核苷酸益生菌胶囊对小鼠衰老的延缓作用。
首先以实施例1中制备的核苷酸益生菌胶囊为例进行实验,取40只快速老化倾向小鼠(SAMP-8小鼠,购自北京维通利华实验动物技术有限公司),体重为(20±5)g,随机分成4组,即空白组(胶囊中仅含有基础饲料)、对照组1(胶囊中仅含有与实施例1的核苷酸益生菌胶囊相同量及相同配比的核苷酸)、对照组2(胶囊中仅含有与实施例1的核苷酸益生菌胶囊相同量及相同配比的益生菌)、试验组(实施例1的核苷酸益生菌胶囊),每组10只,以美国营养学会(American Institute of Nutrition,AIN-93G)动物饲料作为基础饲料,适应性喂养一周,试验分别饲喂空白组、对照组和试验组对应的胶囊,每只SAMP-8小鼠每次2粒,每天3次,连续给药30天,每天记录小鼠状态。
根据结果可知,空白组和对照组1-2随着时间延长,小鼠出现精神状态不佳、活动缓慢的情况,出现衰老迹象,而饲喂本发明的核苷酸益生菌胶囊的试验组小鼠精神和健康状况良好。
考察末次给药后,眼底取血,室温静置待血液完全凝固后离心分离血清备用,随后脱臼发处死小鼠,迅速分离肝脏,氯化钠溶液冲洗干净后制备10%组织匀浆,离心,分离的组织匀浆上清液备用。
小鼠血清及肝组织的SOD和GSH-Px活性按照超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的测定参照试剂盒的说明书进行测定,小鼠血清及肝组织的脂质的过氧化物丙二醛(MDA)含量通过硫代巴比妥酸分光法进行测定。实验数据以均值±标准偏差表示,实验结果如表2-4所示。
表2.饲喂实施例1的胶囊后小鼠体内MDA、SOD和GSH-Px含量数据
表3.饲喂实施例2的胶囊后小鼠体内MDA、SOD和GSH-Px含量数据
表4.饲喂实施例3的胶囊后小鼠体内MDA、SOD和GSH-Px含量数据
结果表明,与空白对照组相比,对照组1-2小鼠血清及肝组织中的SOD活性、GSH-Px活性的活性有所提高,小鼠血清及肝组织中MDA含量有所降低;试验组各指标数值明显优于对照组1-2,进一步表明本发明的抗衰老核苷酸益生菌胶囊可协同增强机体清除自由基的能力,减少组织细胞损伤的程度,显著延缓机体衰老的作用,且没有不良反应。
实施例6
试验方法:在同一地区,同一时间邀请500例皮肤出现老化的女性志愿者,年龄45-60岁,平均年龄54岁。
入选标准:皮肤有轻度或中度的皱纹、皮肤干燥,弹性降低,色素沉着、肤色灰暗等现象;并随机分为10组,每组50人,10组志愿者分别对应空白组(胶囊中仅含有等量的葡萄糖)、对照组1(胶囊中仅含有与实施例1的核苷酸益生菌胶囊相同量及相同配比的核苷酸)、对照组2(胶囊中仅含有与实施例1的核苷酸益生菌胶囊相同量及相同配比的益生菌)、对照组3(胶囊中仅含有与实施例2的核苷酸益生菌胶囊相同量及相同配比的核苷酸)、对照组4(胶囊中仅含有与实施例2的核苷酸益生菌胶囊相同量及相同配比的益生菌)、对照组5(胶囊中仅含有与实施例3的核苷酸益生菌胶囊相同量及相同配比的核苷酸)、对照组6(胶囊中仅含有与实施例3的核苷酸益生菌胶囊相同量及相同配比的益生菌)、试验组1(实施例1的核苷酸益生菌胶囊)、试验组2(实施例2的核苷酸益生菌胶囊)、试验组3(实施例3的核苷酸益生菌胶囊),且每组志愿者均使用对应试验产品作为唯一抗衰老和抗氧化产品,每人每天服用2粒,每日3次,为期四周,试验结束后参加试验的志愿者反馈皮肤变化情况和使用感受,效果评价结果如表5所示。
表5.不同组胶囊产品的效果评价结果
组别 | 每组参与人数 | 面部皮肤紧凑 | 精力充沛 | 记忆力改善 | 睡眠质量改善 |
试验组1 | 50 | 46 | 45 | 40 | 42 |
试验组2 | 50 | 45 | 50 | 41 | 46 |
试验组3 | 50 | 49 | 47 | 43 | 47 |
对照组1 | 50 | 34 | 30 | 32 | 33 |
对照组2 | 50 | 15 | 20 | 18 | 21 |
对照组3 | 50 | 37 | 34 | 30 | 38 |
对照组4 | 50 | 20 | 23 | 25 | 22 |
对照组5 | 50 | 39 | 35 | 37 | 36 |
对照组6 | 50 | 25 | 24 | 26 | 25 |
空白组 | 50 | 7 | 13 | 10 | 8 |
上述结果表明,与空白组和对照组1-6相比,本发明核苷酸益生菌胶囊能够更为有效地改善面部皮肤、精力、记忆力以及睡眠质量,从而达到延缓衰老的目的。
对于任何熟悉本领域的技术人员而言,在不脱离本发明技术方案范围情况下,都可利用上述揭示的技术内容对本发明技术方案作出许多可能的变动和修饰,或修改为等同变化的等效实施例。因此,凡是未脱离本发明技术方案的内容,依据本发明的技术实质对以上实施例所做的任何简单修改、等同变化及修饰,均应仍属于本发明技术方案保护的范围内。
Claims (7)
1.一种延缓衰老的核苷酸益生菌胶囊,其特征在于,包括胶囊和内容物,所述内容物包括4种外源核苷酸或者其钠盐形式的混合物和5种益生菌,重量配比为:核苷酸或者其钠盐形式的混合物45~75份,益生菌20~30份;
所述内容物的重量配比为:5’单磷酸胞苷(5’-CMP)10~20份,5’单磷酸腺苷(5’-AMP)20~30份,5’尿苷酸二钠(5’-UMPNa2)5~10份,5’鸟苷酸二钠(5’-GMPNa2)10~15份,嗜酸乳杆菌5-7份,植物乳杆菌5~7份,干酪乳杆菌5~7份,瑞士乳杆菌3~5份,戊糖片球菌2~4份。
2.根据权利要求1所述的核苷酸益生菌胶囊,其特征在于,所述内容物的重量配比为:
5’单磷酸胞苷10份,5’单磷酸腺苷20份,5’尿苷酸二钠5份,5’鸟苷酸二钠10份,嗜酸乳杆菌5份,植物乳杆菌5份,干酪乳杆菌5份,瑞士乳杆菌3份,戊糖片球菌2份;
或者,所述内容物的重量配比为:
5’单磷酸胞苷15份,5’单磷酸腺苷25份,5’尿苷酸二钠8份,5’鸟苷酸二钠10份,嗜酸乳杆菌6份,植物乳杆菌6份,干酪乳杆菌6份,瑞士乳杆菌4份,戊糖片球菌3份;
或者,所述内容物的重量配比为:
5’单磷酸胞苷20份,5’单磷酸腺苷30份,5’尿苷酸二钠10份,5’鸟苷酸二钠15份,嗜酸乳杆菌7份,植物乳杆菌7份,干酪乳杆菌7份,瑞士乳杆菌5份,戊糖片球菌4份。
3.根据权利要求1所述的核苷酸益生菌胶囊,其特征在于,所述益生菌中每种菌种的活菌含量均大于1×107CFU/g。
4.根据权利要求1所述的核苷酸益生菌胶囊,其特征在于,所述胶囊的壁材为麦芽糊精和大豆分离蛋白,所述麦芽糊精和大豆分离蛋白的重量比为1:1~1:2。
5.根据权利要求4所述的核苷酸益生菌胶囊,其特征在于,所述麦芽糊精和大豆分离蛋白的重量比为1:1.5~1:2。
6.权利要求1-5任一项所述的核苷酸益生菌胶囊的制备方法,其特征在于,按配比称量核苷酸和益生菌,混合均匀,获得混合粉,将混合粉装入空心胶囊中,即得核苷酸益生菌胶囊。
7.权利要求1-5任一项所述的核苷酸益生菌胶囊在制备延缓衰老的保健食品和/或药品中的应用。
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110874599.1A CN113615837B (zh) | 2021-07-30 | 2021-07-30 | 一种延缓衰老的核苷酸益生菌胶囊 |
PCT/CN2022/098178 WO2023005453A1 (zh) | 2021-07-30 | 2022-06-10 | 一种延缓衰老的核苷酸益生菌胶囊 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110874599.1A CN113615837B (zh) | 2021-07-30 | 2021-07-30 | 一种延缓衰老的核苷酸益生菌胶囊 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN113615837A CN113615837A (zh) | 2021-11-09 |
CN113615837B true CN113615837B (zh) | 2022-07-01 |
Family
ID=78381907
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202110874599.1A Active CN113615837B (zh) | 2021-07-30 | 2021-07-30 | 一种延缓衰老的核苷酸益生菌胶囊 |
Country Status (2)
Country | Link |
---|---|
CN (1) | CN113615837B (zh) |
WO (1) | WO2023005453A1 (zh) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113615837B (zh) * | 2021-07-30 | 2022-07-01 | 陈玉松 | 一种延缓衰老的核苷酸益生菌胶囊 |
CN114939153B (zh) * | 2022-05-09 | 2023-04-28 | 大连双迪科技股份有限公司 | 一种抗衰老的含有尿苷酸、腺苷酸和酵母肽的组合物及其应用 |
CN115040550A (zh) * | 2022-06-07 | 2022-09-13 | 广东特芯生物科技有限公司 | 一种用于提高免疫球蛋白g的组合物 |
CN115040549A (zh) * | 2022-06-07 | 2022-09-13 | 广东特芯生物科技有限公司 | 一种用于肿瘤的组合物 |
CN115067518A (zh) * | 2022-06-07 | 2022-09-20 | 广东特芯生物科技有限公司 | 一种用于提高肿瘤患者免疫力的组合物 |
CN115568587B (zh) * | 2022-09-08 | 2024-06-11 | 中国热带农业科学院香料饮料研究所 | 复合益生菌及其应用、发酵菠萝蜜原浆及其制备方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1961891A (zh) * | 2006-11-22 | 2007-05-16 | 北京燕京中科生物技术有限公司 | 一种抗辐射组合物 |
CN101147779A (zh) * | 2006-09-21 | 2008-03-26 | 珍奥集团股份有限公司 | 一种含有核苷酸的组合物、其制备方法及其用途 |
CN105287649A (zh) * | 2015-11-23 | 2016-02-03 | 西安木本贞生物科技有限公司 | 一种医用益生菌胶囊 |
CN112043816A (zh) * | 2020-09-07 | 2020-12-08 | 维塔恩(广州)医药有限公司 | 一种抗氧化、抗衰老组合物及其制剂和应用 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1064233C (zh) * | 1993-03-27 | 2001-04-11 | 颜怀玮 | 多核苷酸营养素产品及制造方法 |
CN113615837B (zh) * | 2021-07-30 | 2022-07-01 | 陈玉松 | 一种延缓衰老的核苷酸益生菌胶囊 |
-
2021
- 2021-07-30 CN CN202110874599.1A patent/CN113615837B/zh active Active
-
2022
- 2022-06-10 WO PCT/CN2022/098178 patent/WO2023005453A1/zh unknown
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101147779A (zh) * | 2006-09-21 | 2008-03-26 | 珍奥集团股份有限公司 | 一种含有核苷酸的组合物、其制备方法及其用途 |
CN1961891A (zh) * | 2006-11-22 | 2007-05-16 | 北京燕京中科生物技术有限公司 | 一种抗辐射组合物 |
CN105287649A (zh) * | 2015-11-23 | 2016-02-03 | 西安木本贞生物科技有限公司 | 一种医用益生菌胶囊 |
CN112043816A (zh) * | 2020-09-07 | 2020-12-08 | 维塔恩(广州)医药有限公司 | 一种抗氧化、抗衰老组合物及其制剂和应用 |
Also Published As
Publication number | Publication date |
---|---|
WO2023005453A1 (zh) | 2023-02-02 |
CN113615837A (zh) | 2021-11-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN113615837B (zh) | 一种延缓衰老的核苷酸益生菌胶囊 | |
CN113398144B (zh) | 核苷酸混合物在制备预防或缓解老年肌少症制剂中的应用 | |
TW201609119A (zh) | 減緩化療藥物副作用之組合物 | |
JP7458660B2 (ja) | プロフィラグリンmRNA発現促進剤、セリンパルミトイルトランスフェラーゼmRNA発現促進剤、及びヒアルロン酸合成酵素3mRNA発現促進剤 | |
Ishwarya et al. | Facile synthesis of haemocyanin-capped zinc oxide nanoparticles: Effect on growth performance, digestive-enzyme activity, and immune responses of Penaeus semisulcatus | |
HaÅ et al. | The effect of addition bee pollen to feed mixtures on internal fat of broiler Ross 308 | |
WO2021200216A1 (ja) | 筋肉増強剤 | |
CN112190601A (zh) | 一种改善记忆力、延缓衰老、抗氧化的片剂及其制备方法 | |
KR101595959B1 (ko) | 율피 열수추출물 또는 율피 발효추출물을 함유하는 알레르기성 피부질환 예방, 개선 또는 치료용 조성물 | |
KR101841118B1 (ko) | 세네데스무스 속 미세조류의 추출물을 유효성분으로 함유하는 피부 외용제 조성물 | |
JP2002235084A (ja) | 抗酸化剤 | |
JP2017001961A (ja) | 生体抗酸化能賦活剤 | |
CN1582964A (zh) | 一种羊胎保健品及其制备方法 | |
CN108852881A (zh) | 一种含二十八烷醇表面活性剂的制备方法与应用 | |
Coroian et al. | Oxidative stress and haematological response in Rainbow Trout (Oncorhynchus mykiss) fed with brewer yeast (Saccharomyces Cerevisiae) as nutritional supplement | |
JP2003238437A (ja) | 抗酸化能を有する発酵生薬製剤 | |
Chen et al. | Effects of various selenium-enriched yeasts, selenomethionine, and nanoselenium on production performance, quality, and antioxidant capacity in laying hens | |
JP7455350B2 (ja) | 大気汚染物質によるダメージ抑制剤、化粧料及び飲食品 | |
CN112493487B (zh) | 一种增强人体免疫力的组合物及其制备方法和保健品 | |
JP7387113B2 (ja) | 新規ラクトバチルス属乳酸菌 | |
CN115104727A (zh) | 一种灵芝雨生红球藻提取物胶囊及其应用 | |
CN109393444A (zh) | 利用鸡腿菇固态转化生姜和大麦制备降血糖食品的方法 | |
Al-Gburi et al. | Local Chicken Effect of Adding Anise and Marjoram Seed Powders and Their Mixtures to Iraqi Male Diets on Seminal Plasma Traits | |
CN117205277A (zh) | 具有抗疲劳功能的组合物及其制备方法和应用 | |
Al-Gburi et al. | Effect of Adding Anise and Marjoram Seed Powders and Their Mixtures to Iraqi Local Chicken Male Diets on Seminal Plasma Traits |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20220525 Address after: 116000 No. 64, Chunhe street, Zhongshan District, Dalian City, Liaoning Province Applicant after: Chen Yusong Address before: 116635 No.9 Jinqi Road, advanced equipment manufacturing industrial park, Dalian Economic and Technological Development Zone, Liaoning Province Applicant before: ZHEN-AO GROUP Co.,Ltd. |
|
GR01 | Patent grant | ||
GR01 | Patent grant |