CN113615715B - Plant source insect pest repellent preparation and application thereof - Google Patents
Plant source insect pest repellent preparation and application thereof Download PDFInfo
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- CN113615715B CN113615715B CN202110910826.1A CN202110910826A CN113615715B CN 113615715 B CN113615715 B CN 113615715B CN 202110910826 A CN202110910826 A CN 202110910826A CN 113615715 B CN113615715 B CN 113615715B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses a botanical insect pest repellent preparation which contains pummelo peel fruit extract, wherein the pummelo peel fruit extract is obtained by taking pummelo peel fruits as raw materials and adopting organic solvent for extraction, and the organic solvent is ethanol, petroleum ether or water. Also discloses application of the plant-source insect pest repellent preparation as an oviposition repellent agent of bactrocera dorsalis or a feeding-repellent agent of spodoptera exigua larvae.
Description
Technical Field
The invention belongs to the technical field of biological control, and particularly relates to a botanical insect pest repellent preparation and application thereof.
Background
Over the past decades, chemically synthesized pesticides have been widely used for pest control, resulting in accumulation of toxic residues in food, pollution of water and soil, poisoning of farmers, and even generation of resistant pests. Nowadays, people pay more and more attention to the demand for substances which are harmful to human health and environment. There are many plants that have pesticidal activity and these plants should be studied and, where possible, introduced into agricultural traits as an alternative to controlling pests.
Beet armyworm is a worldwide intractable pest, has a wide range of feeding impurities and hosts, and is a harmful crop species. In recent years, various crops have been subjected to intermittent outbreaks and disasters. In serious land, the leaves of the plants are completely eaten, and great economic loss is often caused if the control is not timely carried out. At present, chemical control is still the main control for beet armyworm.
The citrus fruit fly is mainly produced in fruits by female insects, the larvae eat pulp, the pulp is rotted, the fruits are immature and fall, the quality and the yield of the fruits are seriously influenced, huge economic loss is brought to the fruit and vegetable planting industry in China, the species of citrus fruit fly hosts are various, the migratory flight capability is strong, the citrus fruit fly is easy to propagate or spread in a large range, high importance is brought to all countries in the world at present, a large amount of manpower and financial resources are invested to control the citrus fruit fly, and meanwhile, a large amount of novel control measures are invested in scientific fund research. With the rapid development and technical improvement of scientific research, the prevention and control technology of bactrocera dorsalis has new development and breakthrough. The current research has been directed to the development and utilization of biological control and trapping technologies, or the combination of several of the above control methods, from early physical, quarantine and agricultural control to chemical control.
There is a need to develop a novel plant-derived insect pest repellent preparation for oviposition repellent of bactrocera dorsalis or novel antifeedant repellent of spodoptera exigua.
At present, the pummelo peel extract is not used in the egg laying repellent of citrus fruit flies and the novel anti-feeding repellent of beet armyworms, so the pummelo peel extract has potential to be developed into a plant source insect pest repellent preparation used in the egg laying repellent of citrus fruit flies or the novel anti-feeding repellent of beet armyworms.
Disclosure of Invention
The invention aims to provide a plant-derived insect pest repellent preparation which has good egg laying repellent effect on citrus fruit flies and good food refusing repellent effect on beet armyworms.
The invention also aims to provide application of the plant-source insect pest repellent preparation as an egg laying repellent for bactrocera dorsalis or a feeding repellent for asparagus caterpillar larvae.
The first object of the present invention can be achieved by the following technical solutions: a plant-derived insect pest repellent preparation contains exocarpium Citri Grandis fruit extract obtained by extracting exocarpium Citri Grandis fruit with organic solvent such as ethanol, petroleum ether or water.
As a preferred technical solution of the present invention, the pummelo peel extract is prepared by the following method:
(1) selecting pummelo peel fruits, slicing, soaking in an organic solvent, extracting by ultrasonic wave after soaking, performing rotary evaporation concentration on an extracting solution, and recovering the organic solvent to obtain a paste concentrate and a recovered organic solvent;
(2) and repeatedly extracting the pummelo peel fruits twice by using the recovered organic solvent, and combining the paste concentrates for three times to obtain the pummelo peel fruit extract.
The preparation method of the pummelo peel fruit extract comprises the following steps:
preferably, the dosage relationship between the pummelo peel slices in the step (1) and the organic solvent is 200-300 g: 1L, and soaking for 6-10 hours by adopting an organic solvent.
More preferably, the dosage relationship between the exocarpium citri grandis fruit slices in the step (1) and the organic solvent is 250 g: 1L, soaking for 8 hours by using an organic solvent.
Preferably, the temperature of ultrasonic extraction in the step (1) is 35-45 ℃, the extraction time is 25-35 min, the power of ultrasonic waves is 100-150W, and the ultrasonic frequency is 40 kHZ.
More preferably, the temperature of ultrasonic extraction in the step (1) is 40 ℃, the extraction time is 30min, the power of ultrasonic wave is 120W, and the ultrasonic frequency is 40 kHZ.
Preferably, the extract in step (1) is concentrated by rotary evaporation until no solution is added in the recovery vessel within 3 min.
The first object of the present invention can be achieved by the following technical solutions: the plant-source insect pest repellent preparation is applied as an egg laying repellent for bactrocera dorsalis or a feeding repellent for beet armyworm larvae.
Earlier researches of the invention find that various insect pests of pummelo peel fruits in Guangdong region of China are less infected in the growing process, which indicates that the pests can avoid biting the pummelo peel fruits as much as possible, but the principle is not clear.
Therefore, the method adopts the pummelo peel fruits as the raw material in an attempt manner, adopts the organic solvent to extract and obtain the pummelo peel fruit extract, is used for testing the egg laying and avoiding effects of the pummelo peel fruits and the antifeedant and repellent effects of the beet armyworm larvae, and results show that the ethanol extract of the pummelo peel fruits has good egg laying and avoiding effects on the pummelo peel flies, and the petroleum ether extract of the pummelo peel fruits has obvious antifeedant and repellent effects on the beet armyworm larvae.
In a preferred embodiment of the present invention, when the pummelo peel fruit extract is used as a bactrocera dorsalis egg-laying repellent, the pummelo peel fruit extract is an ethanol extract of pummelo peel fruit.
Preferably, when the exocarpium citri grandis fruit extract is used as the bactrocera dorsalis egg-laying repellent, the ethanol extract of the exocarpium citri grandis fruit is diluted by ethanol to obtain a diluent, and each 1mL of the diluent contains 40-60 mg of ethanol extract of the exocarpium citri grandis fruit.
More preferably, when the exocarpium citri grandis fruit extract is used as an egg laying repellent for bactrocera dorsalis, the ethanol extract of the exocarpium citri grandis fruit is diluted by ethanol to obtain a diluent, and each 1mL of the diluent contains 50mg of the ethanol extract of the exocarpium citri grandis fruit.
In another preferred embodiment of the present invention, when the pummelo peel fruit extract is used as the anti-feeding repellent for the beet armyworm larvae, the pummelo peel fruit extract is petroleum ether extract of pummelo peel fruit.
Preferably, when the pummelo peel fruit extract is used as the anti-feeding repellent for the beet armyworm larvae, the petroleum ether ethanol extract of the pummelo peel fruit is diluted by petroleum ether to obtain a diluent, and each 1mL of the diluent contains 15-25 mg of the petroleum ether extract of the pummelo peel fruit.
More preferably, when the pummelo peel fruit extract is used as the anti-feeding repellent for the asparagus caterpillar larvae, the petroleum ether ethanol extract of the pummelo peel fruit is diluted by petroleum ether, and each 1mL of the diluted solution contains 20mg of the petroleum ether extract of the pummelo peel fruit.
Compared with the prior art, the invention has the following advantages:
(1) the ethanol extract of the exocarpium citri grandis fruit of the invention has good effect of avoiding egg laying of bactrocera dorsalis;
(2) the petroleum ether extract of pummelo peel fruits has obvious antifeedant repellent effect on beet armyworm larvae;
(3) the insect repellent prepared by pure plants has stable components, simple preparation method, safety to people and livestock, no environmental pollution and wide application prospect.
Detailed Description
The following starting materials are not commercially available unless otherwise specified.
Example 1
The plant-derived pest repellent preparation provided by the embodiment contains pummelo peel fruit extract, and the pummelo peel fruit extract is obtained by taking pummelo peel fruits as raw materials and adopting ethanol extraction.
The pummelo peel fruit extract is prepared by the following method:
(1) weighing 250g of pummelo peel slices, soaking the slices in 1L of ethanol for 8 hours, then carrying out ultrasonic extraction for half an hour at 40 ℃, collecting solution at ultrasonic frequency of 40kHZ and power of 120W, carrying out rotary evaporation concentration on the extracting solution until no solution is added in a recovery container within 3min, and collecting the recovered solution to obtain paste concentrate and recovered solution;
(2) and (3) slicing the recovered and soaked pummelo peel, repeating the step (1) twice to obtain the pummelo peel concentrate for three times, and diluting the pummelo peel concentrate with a corresponding solution as required when in use.
Example 2
The plant-derived pest repellent preparation provided by the embodiment contains pummelo peel fruit extract, and the pummelo peel fruit extract is obtained by taking pummelo peel fruits as raw materials and extracting with petroleum ether.
The pummelo peel fruit extract is prepared by the following method:
(1) weighing 250g of pummelo peel slices, soaking the pummelo peel slices in 1L of petroleum ether for 8 hours, then carrying out ultrasonic extraction for half an hour at the extraction temperature of 40 ℃, collecting solution with the ultrasonic frequency of 40kHZ and the ultrasonic power of 120W, carrying out rotary evaporation concentration on the extracting solution until no solution is added in a recovery container within 3min, and collecting the recovery solution to obtain paste concentrate and recovery solution;
(2) and (3) soaking the recovered solution into pummelo peel slices, repeating the step (1) twice to obtain pummelo peel fruit concentrate for three times, and diluting the pummelo peel fruit concentrate with corresponding solution as required when in use.
Example 3
The plant-derived pest repellent preparation provided by this embodiment contains pummelo peel fruit extract, which is obtained by extracting pummelo peel fruit as a raw material with an organic solvent, and the organic solvent is water.
The pummelo peel fruit extract is prepared by the following method:
(1) weighing 250g of pummelo peel slices, soaking the slices in 1L of water for 8 hours, then extracting the slices by using ultrasonic waves, wherein the extraction time is half an hour, the extraction temperature is 40 ℃, the power of the ultrasonic waves is 120W, the ultrasonic frequency is 40kHZ, collecting a solution, performing rotary evaporation concentration on an extracting solution in the step (1) until no solution is added in a recovery container within 3min, and collecting a recovery solution to obtain a paste concentrate and a recovery solution;
(2) and (3) soaking the recovered solution into pummelo peel slices, repeating the step (1) twice to obtain pummelo peel fruit concentrate for three times, and diluting the pummelo peel fruit concentrate with corresponding solution as required when in use.
Example 4
1. Ethanol extract of exocarpium Citri Grandis fruit as oviposition repellent of Bactrocera dorsalis
The pummelo peel fruit concentrates prepared in examples 1-3 are respectively diluted into pummelo peel fruit extract with the concentration of 50mg/mL by using corresponding solvents, and the ethanol diluent, the petroleum ether diluent and the aqueous solution of the pummelo peel fruit concentrates are respectively obtained by using the corresponding solvents as the dilution solvents.
The citrus fruit fly is collected from the agricultural institute of agricultural engineering, ZhongCao, and cultured at 26 deg.C and 65% of proper temperature, and adult with peak spawning stage is selected for experiment.
Egg-laying approach-avoidance experiment of bactrocera dorsalis
A square cage of 30X 30 is filled with 5 female insects and 5 male insects, and then the basic physiological activity is maintained by the feed. Acclimatization was carried out for 24 hours.
Selecting and purchasing fresh guavas from the market, cleaning and removing pesticide residues, spraying a pummelo peel fruit extract sample on the guavas by an experimental group, spraying a corresponding extraction solvent on a control group, placing opposite corners of the sprayed guavas in a cage, and collecting fruits after 24 hours to count the egg laying situation of the bactrocera dorsalis.
TABLE 1 egg laying avoidance Rate of bactrocera dorsalis in the experimental group with a concentration of 50mg/mL
| Egg laying avoidance rate of 50mg/mL bactrocera dorsalis | Sample (I) | Control | Avoidance rate |
| Ethanol extraction as in example 1 | 40±22 | 66±11 | 24.50％ |
| Petroleum Ether extraction in example 2 | 120±26 | 178±69 | 19.10％ |
| Example 3 Water extraction | 157±49 | 200±99 | 15.20％ |
The results in table 1 show that ethanol extract of exocarpium citri grandis fruit is the best for egg avoidance of bactrocera dorsalis, followed by petroleum ether extract, and finally water extract.
2. Petroleum ether extract of exocarpium Citri Grandis fruit can be used as repellent for beet armyworm larvae
The pummelo peel fruit concentrates prepared in examples 1-3 are respectively diluted into pummelo peel fruit extract with a concentration of 20mg/mL by using corresponding solvents, and the ethanol diluent, the petroleum ether diluent and the aqueous solution of the pummelo peel fruit concentrates are respectively obtained by using the corresponding solvents as the dilution solvents.
Beet armyworm is purchased from a Coyun organism and irradiated at 26 ℃ with a light rhythm of 16L: 8D, humidity of 65%, selecting and using 3-instar beet armyworm larvae for experiments.
Leaf disks with the diameter of 6cm are pricked from vegetable leaves by using a punch needle, an experimental group of the leaf disks (comprising ethanol concentrate diluent of the pummelo peel fruit in example 1, petroleum ether diluent of the pummelo peel fruit concentrate in example 2 and aqueous solution of the pummelo peel fruit concentrate in example 3) is sprayed with pummelo peel extract, a control group is sprayed with corresponding solvents, the three groups of the test leaf disks are respectively placed in a culture dish after being naturally dried, one leaf disk is placed in one culture dish, 5 spodoptera exigua larvae are loaded on one leaf disk, and the weight of the leaf disks and the weight of the larvae are weighed after three groups of the test leaf disks are performed in parallel for 24 hours and 48 hours.
The food refusal rate (%) is (control leaf weight treatment-experimental group leaf weight)/control leaf weight × 100.
The weight change rate (%) (control test insect weight-test group test insect weight)/control test insect weight × 100.
The antifeedant rate of beet armyworm in the experimental group with the concentration of 20mg/mL is shown in the following table 2, and the weight change rate of beet armyworm in the experimental group with the concentration of 20mg/mL is shown in the following table 3.
TABLE 2 beet armyworm antifeedant rate in the experimental group with a concentration of 20mg/mL
| Food refusal rate of beet armyworm of 20mg/mL | 24h | 48h |
| Ethanol extraction as in example 1 | 4.42％ | 74.83% (5 deaths) |
| Petroleum Ether extraction in example 2 | 18.27％ | 78.20% (death 6) |
| Example 3 Water extraction | -2.74％ | 72.80% (dead 3) |
TABLE 3 weight change rate of beet armyworm in the experimental group at a concentration of 20mg/mL
| Food refusal rate of beet armyworm of 20mg/mL | 24h | 48h |
| Ethanol extraction as in example 1 | -210.96％ | 74.59％ |
| Petroleum Ether extraction in example 2 | -141.50％ | -246.97％ |
| Example 3 Water extraction | -16.18％ | -24.62％ |
The data in tables 2 and 3 indicate that: the petroleum ether extract of exocarpium Citri Grandis fruit has the best effect on resisting and repelling the larvae of beet armyworm, and is the petroleum ether extract, and is the water extract.
The invention is not limited to the specific embodiments described above, which are intended to illustrate the use of the invention in detail, and functionally equivalent production methods and technical details are part of the disclosure. Indeed, those skilled in the art can, based on the foregoing description, find various modifications as may be required and which are within the scope of the claims appended hereto.
Claims (7)
1. The application of the plant source insect pest repellent preparation as an egg laying repellent of bactrocera dorsalis hendel;
the plant source insect pest repellent preparation contains pummelo peel fruit extract, wherein the pummelo peel fruit extract is obtained by taking pummelo peel as a raw material and extracting with organic solvent or water, and the organic solvent is ethanol or petroleum ether.
2. Use according to claim 1, characterized in that: the pummelo peel fruit extract is prepared by the following method:
(1) selecting pummelo peel fruits, slicing, soaking in an organic solvent, extracting by ultrasonic wave after soaking, performing rotary evaporation concentration on an extracting solution, and recovering the organic solvent to obtain a paste concentrate and a recovered organic solvent;
(2) and repeatedly extracting the pummelo peel fruits twice by using the recovered organic solvent, and combining the paste concentrates for three times to obtain the pummelo peel fruit extract.
3. Use according to claim 2, characterized in that: in the step (1), the dosage relationship between the pummelo peel slices and the organic solvent is 200-300 g: 1L; in the step (1), the organic solvent is adopted for soaking for 6-10 hours.
4. Use according to claim 2, characterized in that: in the step (1), the temperature is 35-45 ℃ when ultrasonic extraction is adopted, the extraction time is 25-35 min, the power of ultrasonic waves is 100-150W, and the ultrasonic frequency is 40 kHZ.
5. Use according to claim 2, characterized in that: and (2) performing rotary evaporation concentration on the extracting solution in the step (1) until no solution is added in a recovery container within 3 min.
6. Use according to claim 1, characterized in that: when the pummelo peel fruit extract is used as the bactrocera dorsalis egg laying repellent, the pummelo peel fruit extract is an ethanol extract of pummelo peel fruit.
7. Use according to claim 6, characterized in that: when the exocarpium citri grandis fruit extract is used as an egg laying repellent for bactrocera dorsalis, diluting the ethanol extract of the exocarpium citri grandis fruit with ethanol to obtain a diluent, wherein each 1mL of the diluent contains 40-60 mg of the ethanol extract of the exocarpium citri grandis fruit.
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| CN105766494A (en) * | 2014-12-24 | 2016-07-20 | 中国医学科学院药用植物研究所 | Product for preventing plants from freezing damage and pests and weeds and use of product |
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| CN112970789B (en) * | 2021-03-02 | 2021-12-07 | 广西壮族自治区药用植物园 | Extraction method of pummelo peel volatile oil, product and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105766494A (en) * | 2014-12-24 | 2016-07-20 | 中国医学科学院药用植物研究所 | Product for preventing plants from freezing damage and pests and weeds and use of product |
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