CN113603523A - Preparation and application method of vigorous-growth-controlling fertilizer - Google Patents

Preparation and application method of vigorous-growth-controlling fertilizer Download PDF

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CN113603523A
CN113603523A CN202110757822.4A CN202110757822A CN113603523A CN 113603523 A CN113603523 A CN 113603523A CN 202110757822 A CN202110757822 A CN 202110757822A CN 113603523 A CN113603523 A CN 113603523A
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fermentation
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CN113603523B (en
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王怀明
唐世建
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Henan Zhengda Crop Protection Co ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/40Treatment of liquids or slurries
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/80Separation, elimination or disposal of harmful substances during the treatment
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    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/22Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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Abstract

The invention relates to the technical field of fertilizer preparation, and discloses a preparation method and an application method of a vigorous-growth-controlling fertilizer. The invention can effectively control the crops vigorously, has positive effects on reproductive growth, green synthesis of leaves and photosynthesis of the crops, can obviously improve the photosynthesis of the crops after being compared with paclobutrazol and phosphorus-potassium liquid fertilizers in the prior art, thereby achieving the purpose of increasing the yield.

Description

Preparation and application method of vigorous-growth-controlling fertilizer
Technical Field
The invention relates to the technical field of fertilizer preparation, in particular to a preparation method and an application method of a vigorous-growth-controlling fertilizer.
Background
In the planting process of crops, the contradiction between vegetative growth and reproductive growth and the growth of overground parts and underground parts can be met. The harm caused by the vigorous growth of crops in the actual production process is very huge. Vegetables in a vigorous growth period, such as vegetables in a flowering and fruit setting period, have the phenomena of little fruit setting, slow fruit swelling or flower and fruit dropping. The main reasons for the above phenomena are unbalanced vegetative growth and reproductive growth, over-vigorous vegetative growth, suppressed reproductive growth, organic nutrition which is transported to branches and leaves but not to flowers and fruits, thereby causing poor flower bud differentiation and fruit setting. Even if the fruit is set, the fruit tends to be misshapen, or even not swelled. And the reproductive growth is over-vigorous, and the plants consume excessive nutrients, which can cause premature senility of the vegetables. After the fruit trees enter the reproductive growth period, if the vegetative growth is too vigorous, the fruit setting is less, the fruit swelling is slow, and finally, the yield is low, the quality is poor and the income is influenced.
The current vigorous growth control products on the market are basically plant growth regulators, mainly comprise chlormequat chloride, paclobutrazol and mepiquat chloride, act as antagonists of gibberellin synthetic substances, belong to growth retardants, weaken the growth vigor of crops and do not influence cell division. But the existing regulator has obvious defect of vigorous control, firstly, the use safety technical requirement is higher, and the pesticide damage is easy to occur when the concentration is not suitable for use; secondly, the residual time of partial components is long, which has influence on the next crop; thirdly, the use of the regulator product is easy to cause premature senility of crops and influence the yield and the quality.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a preparation method and an application method of a vigorous growth control fertilizer, which have the advantages of vigorous growth control, photosynthesis promotion, cost reduction, harm reduction and the like, and solve the problems in the background art.
(II) technical scheme
In order to achieve the purposes of controlling vigorous growth, promoting photosynthesis, reducing cost and reducing harmfulness, the invention provides the following technical scheme:
another technical problem to be solved by the invention is to provide a preparation method and an application method of a vigorous growth controlling fertilizer, wherein the vigorous growth controlling fertilizer comprises the components of cord-grass, oldenlandia diffusa, bacillus subtilis (LSK16), DL-malic acid, mineral nutrient elements, organic acid and sugar alcohol; the weight parts of the components are as follows: 5 to 20 percent of biological phenol, 20 to 50 percent of mineral nutrient elements, 2 to 10 percent of organic acid and sugar alcohol, and water to be supplemented to 100 percent
The preparation method of the vigorous growth control fertilizer comprises the following steps:
1) the method comprises the steps of crushing and mixing the cord-grass and the oldenlandia according to a ratio of 9:1, then adding medium trace elements, carbon sources and nitrogen sources required by microbial fermentation, uniformly mixing the trace elements, the carbon sources and the nitrogen sources with the crushed and mixed raw materials, then carrying out high-temperature sterilization, and finally inoculating bacillus subtilis (LSK 16).
2) Sufficient oxygen supply is ensured in the initial fermentation stage, after 8 hours of fermentation, the oxygen supply is reduced by reducing the speed of introducing gas into the fermentation tank, and DL-malic acid is added at the same time, wherein the addition amount is 0.5-2% of the total fermentation amount.
3) After fermenting for 20 hours, recovering oxygen supply to ensure sufficient oxygen supply, and stopping fermentation when the pH reaches 6.5, namely finishing the fermentation; and after the fermentation is finished, removing solid matters, concentrating the fermentation liquor to a Baume degree of 20 degrees (the error is not more than 0.2 degrees), and mechanically preparing the biological phenol raw material.
4) Adding the biological phenol raw material and water for supplementing into a reaction kettle, uniformly stirring, adding mineral elements such as phosphorus, potassium, boron and the like, sugar alcohol and organic acid, heating to 80 ℃, keeping the temperature for reaction for 2 hours, and naturally cooling to normal temperature to obtain the biological phenol organic sugar alcohol.
Preferably, the high-temperature sterilization temperature is 115-125 ℃, and the sterilization time is 25-30 min; the oxygen supply amount at the initial stage of fermentation is 0.7m3/min, and the minimum oxygen supply amount after the oxygen supply amount is reduced is not less than 0.5m 3/min.
Preferably, the organic acid is one or a combination of more than two of citric acid, malic acid, gluconic acid, acetic acid, benzoic acid and salicylic acid.
Preferably, the sugar alcohol is one or a combination of more than two of sorbitol, xylitol, mannitol, erythritol and maltitol.
Preferably, the application method of the vigorous-growth-controlling fertilizer comprises the following steps: the vigorous-growth-controlling fertilizer is diluted by adding water, the dilution multiple is 300-500 times, the diluted liquid is sprayed on the leaf surfaces of crops by using a spraying device, the interval time between two adjacent spraying is 10-15 days, and the spraying frequency of the same crop is 2-3 times.
(III) advantageous effects
Compared with the prior art, the invention provides a preparation method and an application method of a vigorous-growth-controlling fertilizer, and the vigorous-growth-controlling fertilizer has the following beneficial effects:
1. the preparation method and the application method of the vigor-controlling fertilizer can effectively control vigor of crops, have positive effects on reproductive growth of the crops, green synthesis of leaves and photosynthesis, and can obviously improve the photosynthesis of the crops after being compared with paclobutrazol and phosphorus-potassium liquid fertilizers in the prior art, thereby achieving the purpose of increasing yield.
2. The preparation and application method of the vigorous-growth-controlling fertilizer are characterized in that most of the adopted raw materials are harmless raw materials, when the growth of crops is completed, the harmfulness of the product can be effectively reduced, the use safety is ensured, meanwhile, the microbial fermentation conversion process is utilized, the extraction efficiency is increased, the molecular spectrum of effective components is enlarged, the action effect is milder, beneficial components such as microbial metabolites and mycoprotein and the like are added to the product, the product not only has vigorous control, but also has the growth promoting function, the effect is ensured, the cost of synergistic raw materials is greatly reduced, the top end advantage can be obviously inhibited after the fertilizer is used, the flower bud differentiation is promoted, the young fruit is expanded, the inhibition is also simple and convenient to remove, only watering and nitrogen fertilizer application are needed, and the fertilizer is convenient and simple to use in agriculture.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
in a 1000L fermentation tank, 600kg of water is added, and 80kg (on a dry basis) of a material obtained by pulverizing and mixing Spartina alterniflora and Hedyotis diffusa at a ratio of 9:1 is added, wherein the ratio of the material to the material is 1: 4:9:2: adding fermentation adjuvants such as magnesium sulfate, calcium carbonate, sodium chloride, glucose, soybean protein powder, potassium dihydrogen phosphate, and defoaming agent at a ratio of 1:1, wherein the total mass of the fermentation adjuvants is 0.5kg, and sterilizing at 121 deg.C for 30 min. Cooling to 35 deg.C, inoculating Bacillus subtilis (auxotroph), controlling temperature at 35 deg.C, ventilating amount at 0.7m3/min, and stirring at 120 r/min. Fermenting for 8 hr, reducing ventilation to 0.5m3/min, and adding sterilized DL-malic acid 2kg every 5min, for a total of 40 kg. After 20 hours of fermentation, the aeration rate was returned to 0.7m 3/min. Fermenting for 24 hours, sampling every half hour to observe the growth condition of the thalli, and if microscopic examination finds that the number of the thalli is reduced, detecting the fermentation liquor by using an ultraviolet spectrophotometer. The absorbance of the fermentation was measured at 339nm and 323nm, respectively. The fermentation broth was diluted 1000-fold, and the fermentation was stopped when the absorbance at 339nm was 0.85 or more and the absorbance at 323nm was 0.60 or more. Removing solid substances from the fermentation liquor, decolorizing with active carbon, and concentrating at low temperature to about 20 ° Baume degree to obtain biological phenol material; then the nitrogen, phosphorus and potassium are prepared according to a formula of 0-200-300g/L + TE, and the mixture ratio is as shown in the following table
Material(s) Proportioning (kg)
Potassium dihydrogen phosphate 30
Potassium hydroxide 15
Boric acid 2.5
Ammonium molybdate 0.1
Biological phenol raw material 15
Mannitol 2.5
DL-malic acid 5
Water (W) 29.9
Putting the materials into a reaction kettle, heating to 80 ℃, reacting for 2 hours, and cooling to obtain the product. The product is applied to citrus, and can be used for controlling the summer tip of citrus and improving fruit setting and swelling. The test data are as follows:
the use period is as follows: the first use is started when spring is seven and eight minutes old (more than Xiahua 2/3).
Figure 2
By using the product of the invention, the growth of the citrus in spring and summer is obviously inhibited, the citrus is aged before spring, the growth of leaves, fruits and root systems is only not inhibited but is promoted, and compared with a control product, the product of the invention is obviously superior to a control group in fruit setting rate and fruit swelling. The invention has the advantages of other vigorous growth control products, has vigorous growth control and growth promotion functions, and obviously improves the economic benefit and the social benefit.
Example two:
in a 1000L fermentation tank, 600kg of water is added, and 80kg (on a dry basis) of a material obtained by pulverizing and mixing Spartina alterniflora and Hedyotis diffusa at a ratio of 9:1 is added, wherein the ratio of the material to the material is 1: 4:9:2: adding fermentation adjuvants such as magnesium sulfate, calcium carbonate, sodium chloride, glucose, soybean protein powder, potassium dihydrogen phosphate, and defoaming agent at a ratio of 1:1, wherein the total mass of the fermentation adjuvants is 0.5kg, and sterilizing at 125 deg.C for 25 min. Cooling to 35 deg.C, inoculating Bacillus subtilis (auxotroph), controlling temperature at 35 deg.C, ventilating amount at 0.7m3/min, and stirring at 120 r/min. Fermenting for 8 hr, reducing ventilation to 0.5m3/min, and adding sterilized DL-malic acid 2kg every 5min, for a total of 40 kg. After 20 hours of fermentation, the aeration rate was returned to 0.7m 3/min. Fermenting for 24 hours, sampling every half hour to observe the growth condition of the thalli, and if microscopic examination finds that the number of the thalli is reduced, detecting the fermentation liquor by using an ultraviolet spectrophotometer. The absorbance of the fermentation was measured at 339nm and 323nm, respectively. The fermentation broth was diluted 1000-fold, and the fermentation was stopped when the absorbance at 339nm was 0.85 or more and the absorbance at 323nm was 0.60 or more. Removing solid substances from the fermentation liquor, decolorizing with active carbon, and concentrating at low temperature to about 20 ° Baume degree to obtain biological phenol material;
then the nitrogen, phosphorus and potassium are prepared according to a formula of 0-200-300g/L + TE, and the mixture ratio is as shown in the following table
Material(s) Proportioning (kg)
Potassium dihydrogen phosphate 40
Potassium hydroxide 20
Boric acid 2.5
Ammonium molybdate 0.1
Biological phenol raw material 10
Mannitol 2
Sorbitol 5
DL-malic acid 3.5
Glutamic acid sodium salt 2
Water (W) 14.9
Putting the materials into a reaction kettle, heating to 80 ℃, reacting for 2 hours, and cooling to obtain a product; the product is applied to cucumbers and is used for controlling the vine growth of the cucumbers and improving fruit setting and fruit swelling, and test data are shown in the following table.
The use period is as follows: the melon vine is about 30cm, and the first female flower appears.
Figure BDA0003148500120000081
The product of the invention is mainly used for the cucumber to show that the cucumber internode is shortened, the female flower differentiation is obvious, the quantity is large, the fruit swelling effect is good, the cucumber strips are straight, long, green and many times, the appearance is good, the taste is good, the yield and the quality of the cucumber are obviously improved compared with those of a control group, and the economic benefit and the social benefit are obvious.
Example three:
in a 1000L fermentation tank, 600kg of water is added, and 80kg (on a dry basis) of a material obtained by pulverizing and mixing Spartina alterniflora and Hedyotis diffusa at a ratio of 9:1 is added, wherein the ratio of the material to the material is 1: 4:9:2: adding fermentation adjuvants such as magnesium sulfate, calcium carbonate, sodium chloride, glucose, soybean protein powder, potassium dihydrogen phosphate, and defoaming agent at a ratio of 1:1, wherein the total mass of the fermentation adjuvants is 0.5kg, and sterilizing at 115 deg.C for 30 min. Cooling to 35 deg.C, inoculating Bacillus subtilis (auxotroph), controlling temperature at 35 deg.C, ventilating amount at 0.7m3/min, and stirring at 120 r/min. Fermenting for 8 hr, reducing ventilation to 0.5m3/min, and adding sterilized DL-malic acid 2kg every 5min, for a total of 40 kg. After 20 hours of fermentation, the aeration rate was returned to 0.7m 3/min. Fermenting for 24 hours, sampling every half hour to observe the growth condition of the thalli, and if microscopic examination finds that the number of the thalli is reduced, detecting the fermentation liquor by using an ultraviolet spectrophotometer. The absorbance of the fermentation was measured at 339nm and 323nm, respectively. The fermentation broth was diluted 1000-fold, and the fermentation was stopped when the absorbance at 339nm was 0.85 or more and the absorbance at 323nm was 0.60 or more. Removing solid substances from the fermentation liquor, decolorizing with active carbon, and concentrating at low temperature to about 20 ° Baume degree to obtain biological phenol material;
then the nitrogen, phosphorus and potassium are prepared according to a formula of 0-200-300g/L + TE, and the mixture ratio is as shown in the following table
Figure BDA0003148500120000091
Figure BDA0003148500120000101
Putting the materials into a reaction kettle, heating to 80 ℃, reacting for 2 hours, and cooling to obtain a product; the product is applied to cowpeas, and is used for controlling the overgrowth of the cowpeas and improving the yield. The test data are shown in the table below.
The use period is as follows: climbing the pole starts (about 30cm) leaf spraying.
Figure BDA0003148500120000102
The product of the invention is mainly embodied in the performance of cowpea, the cowpea internode is shortened, the leaf color is dark green, the stem is thick and strong, the cowpea has long and large strips, the cowpea is good in selling, the yield is high, and the economic benefit and the social benefit are very obvious.
Example four:
in a 1000L fermentation tank, 600kg of water is added, and 80kg (on a dry basis) of a material obtained by pulverizing and mixing Spartina alterniflora and Hedyotis diffusa at a ratio of 9:1 is added, wherein the ratio of the material to the material is 1: 4:9:2: adding fermentation adjuvants such as magnesium sulfate, calcium carbonate, sodium chloride, glucose, soybean protein powder, potassium dihydrogen phosphate, and defoaming agent at a ratio of 1:1, wherein the total mass of the fermentation adjuvants is 0.5kg, and sterilizing at 115 deg.C for 30 min. Cooling to 35 deg.C, inoculating Bacillus subtilis (auxotroph), controlling temperature at 35 deg.C, ventilating amount at 0.7m3/min, and stirring at 120 r/min. Fermenting for 8 hr, reducing ventilation to 0.5m3/min, and adding sterilized DL-malic acid 2kg every 5min, for a total of 40 kg. After 20 hours of fermentation, the aeration rate was returned to 0.7m 3/min. Fermenting for 24 hours, sampling every half hour to observe the growth condition of the thalli, and if microscopic examination finds that the number of the thalli is reduced, detecting the fermentation liquor by using an ultraviolet spectrophotometer. The absorbance of the fermentation was measured at 339nm and 323nm, respectively. The fermentation broth was diluted 1000-fold, and the fermentation was stopped when the absorbance at 339nm was 0.85 or more and the absorbance at 323nm was 0.60 or more. Removing solid substances from the fermentation liquor, decolorizing with active carbon, and concentrating at low temperature to about 20 ° Baume degree to obtain biological phenol material;
then the nitrogen, phosphorus and potassium are prepared according to a formula of 0-200-300g/L + TE, and the mixture ratio is as shown in the following table
Material(s) Proportioning (kg)
Potassium dihydrogen phosphate 40
Potassium hydroxide 20
Boric acid 2.5
Ammonium molybdate 0.1
Biological phenol raw material 10
Mannitol 2
Sorbitol 5
DL-malic acid 3.5
Glutamic acid sodium salt 2
Water (W) 14.9
Putting the materials into a reaction kettle, heating to 80 ℃, reacting for 2 hours, and cooling to obtain a product; the product is applied to corn, and is used for controlling the spindling of the corn and improving the yield. The test data are shown in the table below;
the use period is as follows: the corn is used at the flare stage.
Figure 1
The product of the invention is mainly embodied in the performance of corn, the corn internode is shortened, the leaf color is dark green, the stem is thick and strong, the lodging resistance is realized, the corn kernel is full, the weight of the corn kernel is heavier, and the economic benefit and the social benefit are very obvious.
The invention has the beneficial effects that: can effectively control the crops vigorously, has positive effects on reproductive growth of the crops, green synthesis of leaves and photosynthesis, can obviously improve the photosynthesis of the crops after being compared with paclobutrazol and phosphorus-potassium liquid fertilizers in the prior art, thereby achieving the purpose of increasing the yield, adopts most of harmless raw materials, can effectively reduce the harmfulness of products when the crops grow to the maximum extent, ensures the use safety, simultaneously utilizes a microbial fermentation conversion process, increases the extraction efficiency, enlarges the molecular spectrum of effective components, has milder action effect, increases the functions of other aspects of the products by beneficial components such as microbial metabolites, mycoprotein and the like, ensures that the products not only can control the crops vigorously, but also have the growth promoting function, greatly reduces the cost of synergistic raw materials while ensuring the effect, and can obviously inhibit the top advantages after being used, the flower bud differentiation is promoted, the young fruit is expanded, the inhibition is removed, the watering and the application of nitrogen fertilizer are only needed, and the agricultural application is convenient and simple.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (5)

1. A preparation method and an application method of a vigorous-growth-controlling fertilizer are characterized in that: the ingredients of the vigorous-growth-controlling fertilizer comprise cord grass, oldenlandia diffusa, bacillus subtilis (LSK16), DL-malic acid, mineral nutrient elements, organic acid and sugar alcohol; the weight parts of the components are as follows: 5 to 20 percent of biological phenol, 20 to 50 percent of mineral nutrient elements, 2 to 10 percent of organic acid and sugar alcohol, and water to be supplemented to 100 percent
The preparation method of the vigorous growth control fertilizer comprises the following steps:
1) the method comprises the steps of crushing and mixing the cord-grass and the oldenlandia according to a ratio of 9:1, then adding medium trace elements, carbon sources and nitrogen sources required by microbial fermentation, uniformly mixing the trace elements, the carbon sources and the nitrogen sources with the crushed and mixed raw materials, then carrying out high-temperature sterilization, and finally inoculating bacillus subtilis (LSK 16).
2) Sufficient oxygen supply is ensured in the initial fermentation stage, after 8 hours of fermentation, the oxygen supply is reduced by reducing the speed of introducing gas into the fermentation tank, and DL-malic acid is added at the same time, wherein the addition amount is 0.5-2% of the total fermentation amount.
3) After fermenting for 20 hours, recovering oxygen supply to ensure sufficient oxygen supply, and stopping fermentation when the pH reaches 6.5, namely finishing the fermentation; and after the fermentation is finished, removing solid matters, concentrating the fermentation liquor to a Baume degree of 20 degrees (the error is not more than 0.2 degrees), and mechanically preparing the biological phenol raw material.
4) Adding the biological phenol raw material and water for supplementing into a reaction kettle, uniformly stirring, adding mineral elements such as phosphorus, potassium, boron and the like, sugar alcohol and organic acid, heating to 80 ℃, keeping the temperature for reaction for 2 hours, and naturally cooling to normal temperature to obtain the biological phenol organic sugar alcohol.
2. The preparation and application method of the vigorous-growth-controlling fertilizer according to claim 1, characterized in that: the high-temperature sterilization temperature is 115-125 ℃, and the sterilization time is 25-30 min; the oxygen supply amount at the initial stage of fermentation is 0.7m3/min, and the minimum oxygen supply amount after the oxygen supply amount is reduced is not less than 0.5m 3/min.
3. The preparation and application method of the vigorous-growth-controlling fertilizer according to claim 1, characterized in that: the sugar alcohol is one or the combination of more than two of sorbitol, xylitol, mannitol, erythritol and maltitol.
4. The preparation and application method of the vigorous-growth-controlling fertilizer according to claim 1, characterized in that: the organic acid is one or more of citric acid, malic acid, gluconic acid, acetic acid, benzoic acid, and salicylic acid.
5. A preparation method and an application method of a vigorous-growth-controlling fertilizer are characterized in that: the application method of the vigorous-growth-controlling fertilizer comprises the following steps: the vigorous-growth-controlling fertilizer is diluted by adding water, the dilution multiple is 300-500 times, the diluted liquid is sprayed on the leaf surfaces of crops by using a spraying device, the interval time between two adjacent spraying is 10-15 days, and the spraying frequency of the same crop is 2-3 times.
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