CN113599313A - Antibacterial hand sanitizer - Google Patents
Antibacterial hand sanitizer Download PDFInfo
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- CN113599313A CN113599313A CN202110587197.3A CN202110587197A CN113599313A CN 113599313 A CN113599313 A CN 113599313A CN 202110587197 A CN202110587197 A CN 202110587197A CN 113599313 A CN113599313 A CN 113599313A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
Abstract
The invention relates to an antibacterial hand sanitizer, and belongs to the technical field of hand sanitizers. The invention aims to provide a bacteriostatic hand sanitizer. The antibacterial hand sanitizer contains 8-15 wt% of antibacterial active ingredients, wherein the antibacterial active ingredients comprise a first clear decoction dreg extract, a eucalyptus leaf extract and a honeysuckle extract, and the weight ratio of the first clear decoction dreg extract to the eucalyptus leaf extract to the honeysuckle extract is 1-3: 0.5-1.5: 1. The ultrasonic-assisted extraction method is adopted to extract the first-cleaning capsule dregs to obtain the first-cleaning dregs extract, so that the dregs can be recycled, waste materials are changed into things of value, the environment is protected, the first-cleaning capsule dregs can be used as a main bacteriostatic component to prepare the hand sanitizer, the eucalyptus leaf extract and the honeysuckle extract are matched, the components are compatible with each other, and the obtained hand sanitizer has a good bacteriostatic effect and even has a bacteriostatic effect equivalent to that of a raw medicinal material extract.
Description
Technical Field
The invention relates to an antibacterial hand sanitizer, and belongs to the technical field of hand sanitizers.
Background
The hand sanitizer is a skin-care cleansing liquid used for cleaning hands, and dirt and attached bacteria on the hands are removed by mechanical friction and the action of a surfactant in cooperation with water flow. Along with the improvement of living standard of people and the enhancement of health consciousness, the requirement on the hand sanitizer is gradually improved, and the bacteriostatic hand sanitizer capable of better inhibiting the growth of bacteria is produced.
At present, bacteriostatic active ingredients are mostly added in the hand sanitizer to achieve the bacteriostatic effect. Chinese herbal medicines in China have a plurality of bacteriostatic effects, such as a clear formula. A Xiexin Tang from the book of "jin Bian Yao L ü e" of Zhang Zhongjing in east Han Dynasty is recorded in the "Chinese pharmacopoeia". A Yiqing capsule or Yiqing granule is a compound preparation consisting of 3 Chinese medicinal materials of scutellaria root, coptis root and rhubarb, and the traditional process comprises the steps of decocting the three Chinese medicinal materials respectively in water twice, mixing decoctions and concentrating. With the wide clinical application of Yiqing capsules, the market demand is high, and a large amount of waste medicine dregs are generated. At present, more than 90 percent of manufacturers discard, bury and burn the medicine dregs at will, which not only causes low utilization efficiency of traditional Chinese medicine resources and low industrial economic benefit, but also causes environmental pollution.
The Chinese invention patent with the application number of 201711390837.1 discloses an antibacterial and anti-inflammatory hand sanitizer with recycled traditional Chinese medicine dregs, which is prepared from the traditional Chinese medicine dregs, sodium dodecyl sulfate, deionized water, sodium lauroyl amide, cocamidopropyl betaine, potassium sorbate, citric acid and mei teng fruit oil serving as raw materials and a proper amount of auxiliary materials, wherein the traditional Chinese medicine dregs are dregs left after extraction of one or more traditional Chinese medicines of philippine flemingia root, cherokee rose root, suberect spatholobus stem, unifacial pricklyash root, mahonia stem, common andrographis herb, Chinese angelica and pilose asiabell root. The hand sanitizer is prepared by directly drying and crushing traditional Chinese medicine residues and adding the traditional Chinese medicine residues into the hand sanitizer, and the obtained hand sanitizer has rough hand feeling and needs to be improved in bacteriostatic effect.
Disclosure of Invention
Aiming at the defects, the invention provides the antibacterial hand sanitizer.
The antibacterial hand sanitizer contains 8-15 wt% of antibacterial active ingredients, wherein the antibacterial active ingredients comprise a first clear decoction dreg extract, a eucalyptus leaf extract and a honeysuckle extract, and the weight ratio of the first clear decoction dreg extract to the eucalyptus leaf extract to the honeysuckle extract is 1-3: 0.5-1.5: 1;
wherein the first clear decoction dreg extract is obtained by adopting the following method: mixing the Yiqing capsule residues with an ethanol solution, carrying out ultrasonic extraction, carrying out solid-liquid separation, and concentrating the liquid to dryness to obtain a Yiqing capsule residue extract, wherein the feed-liquid ratio of the Yiqing capsule residues to the ethanol solution is 1: 10-25 g/mL; the volume concentration of the ethanol solution is 50-60%; the extraction temperature is 60-80 ℃, and the extraction time is 1-2 h;
the eucalyptus leaf extract is obtained by adopting the following method: mixing the eucalyptus leaves with an ethanol solution, carrying out ultrasonic extraction, carrying out solid-liquid separation, and concentrating the liquid to dryness to obtain a eucalyptus leaf extract, wherein the material-liquid ratio of the eucalyptus leaves to the ethanol solution is 1: 8-12 g/mL; the volume concentration of the ethanol solution is 55-65%; the ultrasonic temperature is 55-65 ℃, and the ultrasonic time is 0.8-1.2 h;
the honeysuckle extract is obtained by adopting the following method: mixing honeysuckle with an ethanol solution, refluxing in a water bath, then carrying out solid-liquid separation, and concentrating the liquid to dryness to obtain a honeysuckle extract, wherein the material-liquid ratio of the honeysuckle to the ethanol solution is 1: 10-14 g/mL; the volume concentration of the ethanol solution is 55-65%; the extraction temperature is 45-55 ℃, and the reflux time is 3-5 h.
In one embodiment of the present invention, the weight ratio of the first clear residue extract, the eucalyptus leaf extract and the honeysuckle extract is 2: 1.
In one embodiment of the invention, the bacteriostatic hand sanitizer contains 12 wt% of bacteriostatic active ingredients.
In a preferred embodiment of the invention, when the Yiqing decoction dreg extract is prepared, the material-liquid ratio of the Yiqing capsule decoction dregs to the ethanol solution is 1:15 g/mL; the volume concentration of the ethanol solution is 60 percent; the extraction temperature is 70 deg.C, and the extraction time is 1 h.
In a preferred embodiment of the invention, the eucalyptus leaf extract is prepared, wherein the feed-liquid ratio of the eucalyptus leaf to the ethanol solution is 1: 10 g/mL; the volume concentration of the ethanol solution is 60 percent; the ultrasonic temperature is 60 ℃, and the ultrasonic time is 1 h.
In a preferred embodiment of the invention, when the honeysuckle extract is prepared, the feed-liquid ratio of the honeysuckle to the ethanol solution is 1:12 g/mL; the volume concentration of the ethanol solution is 60 percent; the extraction temperature is 50 ℃, and the reflux time is 4 h.
In one embodiment of the invention, the power of the ultrasound is 200-250W. In one embodiment of the present invention, the ultrasonic power is 220W.
In a specific embodiment of the invention, the Yiqing capsule dregs are a composition of scutellaria baicalensis dregs, coptis chinensis dregs and rhubarb dregs, and the weight ratio of the scutellaria baicalensis dregs, the coptis chinensis dregs and the rhubarb dregs is 4-6: 8-12: 3-4.
In a specific embodiment of the invention, the weight ratio of the scutellaria baicalensis dregs, the rhubarb dregs and the coptis chinensis dregs is 5: 10: 3.3.
In one embodiment of the invention, the bacteriostatic hand sanitizer further comprises at least one of a surfactant, a humectant, a thickener and an essential oil.
In a specific embodiment of the invention, the antibacterial hand sanitizer comprises the following components in percentage by weight: 10-30% of surfactant, 4-8% of humectant, 1-3% of thickener, 8-15% of antibacterial active ingredient, 0.05-0.2% of mint essential oil and the balance of water. In a specific embodiment of the invention, the antibacterial hand sanitizer comprises the following components in percentage by weight: 20% of surfactant, 6% of humectant, 2% of thickening agent, 12% of bacteriostatic active ingredient, 0.1% of mint essential oil and the balance of water. .
In a specific embodiment of the invention, the surfactant is at least one of sodium fatty alcohol-polyoxyethylene ether sulfate, cocamidopropyl betaine and lauryl glucoside; the humectant is propylene glycol.
In a specific embodiment of the invention, the surfactant is fatty alcohol-polyoxyethylene ether sodium sulfate, cocamidopropyl betaine and lauryl glycoside, and the weight ratio of the fatty alcohol-polyoxyethylene ether sodium sulfate, the cocamidopropyl betaine and the lauryl glycoside is 5-8: 2-4: 2. In a specific example, the weight ratio of sodium fatty alcohol polyoxyethylene ether sulfate, cocamidopropyl betaine, and lauryl glycoside is 6:3: 2.
Compared with the prior art, the invention has the following beneficial effects:
according to the bacteriostatic hand sanitizer, the capsule-clearing decoction dregs are extracted by an ultrasonic-assisted extraction method to obtain the decoction dreg extract, so that the decoction dregs can be recycled, waste materials are turned into wealth, the hand sanitizer is green and environment-friendly, the medicine dreg extract can be used as a bacteriostatic main component to prepare the hand sanitizer, meanwhile, the eucalyptus leaf extract and the honeysuckle extract are matched, and the components are matched with each other, so that the obtained hand sanitizer has a good bacteriostatic effect and even has a bacteriostatic effect equivalent to that of a raw medicinal material extracting solution.
Detailed Description
The antibacterial hand sanitizer contains 8-15 wt% of antibacterial active ingredients, wherein the antibacterial active ingredients comprise a first clear decoction dreg extract, a eucalyptus leaf extract and a honeysuckle extract, and the weight ratio of the first clear decoction dreg extract to the eucalyptus leaf extract to the honeysuckle extract is 1-3: 0.5-1.5: 1;
wherein the first clear decoction dreg extract is obtained by adopting the following method: mixing the Yiqing capsule residues with an ethanol solution, carrying out ultrasonic extraction, carrying out solid-liquid separation, and concentrating the liquid to dryness to obtain a Yiqing capsule residue extract, wherein the feed-liquid ratio of the Yiqing capsule residues to the ethanol solution is 1: 10-25 g/mL; the volume concentration of the ethanol solution is 50-60%; the extraction temperature is 60-80 ℃, and the extraction time is 1-2 h;
the eucalyptus leaf extract is obtained by adopting the following method: mixing the eucalyptus leaves with an ethanol solution, carrying out ultrasonic extraction, carrying out solid-liquid separation, and concentrating the liquid to dryness to obtain a eucalyptus leaf extract, wherein the material-liquid ratio of the eucalyptus leaves to the ethanol solution is 1: 8-12 g/mL; the volume concentration of the ethanol solution is 55-65%; the ultrasonic temperature is 55-65 ℃, and the ultrasonic time is 0.8-1.2 h;
the honeysuckle extract is obtained by adopting the following method: mixing honeysuckle with an ethanol solution, refluxing in a water bath, then carrying out solid-liquid separation, and concentrating the liquid to dryness to obtain a honeysuckle extract, wherein the material-liquid ratio of the honeysuckle to the ethanol solution is 1: 10-14 g/mL; the volume concentration of the ethanol solution is 55-65%; the extraction temperature is 45-55 ℃, and the reflux time is 3-5 h.
According to the bacteriostatic hand sanitizer, the capsule-clearing decoction dregs are extracted by an ultrasonic-assisted extraction method to obtain the decoction dreg extract, so that the decoction dregs can be recycled, waste materials are turned into wealth, the hand sanitizer is green and environment-friendly, the medicine dreg extract can be used as a bacteriostatic main component to prepare the hand sanitizer, meanwhile, the eucalyptus leaf extract and the honeysuckle extract are matched, and the components are matched with each other, so that the obtained hand sanitizer has a good bacteriostatic effect and even has a bacteriostatic effect equivalent to that of a raw medicinal material extracting solution.
In one embodiment of the present invention, the weight ratio of the first clear residue extract, the eucalyptus leaf extract and the honeysuckle extract is 2: 1.
In one embodiment of the invention, the bacteriostatic hand sanitizer contains 12 wt% of bacteriostatic active ingredients.
In one embodiment of the present invention, the first herb residue, the eucalyptus leaves and the honeysuckle are all powders. The powdered raw materials are adopted, so that the extraction can be conveniently carried out to obtain the extract.
In a preferred embodiment of the invention, when the Yiqing decoction dreg extract is prepared, the material-liquid ratio of the Yiqing capsule decoction dregs to the ethanol solution is 1:15 g/mL; the volume concentration of the ethanol solution is 60 percent; the extraction temperature is 70 deg.C, and the extraction time is 1 h.
In a preferred embodiment of the invention, the eucalyptus leaf extract is prepared, wherein the feed-liquid ratio of the eucalyptus leaf to the ethanol solution is 1: 10 g/mL; the volume concentration of the ethanol solution is 60 percent; the ultrasonic temperature is 60 ℃, and the ultrasonic time is 1 h.
In a preferred embodiment of the invention, when the honeysuckle extract is prepared, the feed-liquid ratio of the honeysuckle to the ethanol solution is 1:12 g/mL; the volume concentration of the ethanol solution is 60 percent; the extraction temperature is 50 ℃, and the reflux time is 4 h.
The invention adopts ultrasonic auxiliary extraction, and the ultrasonic power commonly used in the field can be applied to the invention. In one embodiment of the invention, the power of the ultrasound is 200-250W. In one embodiment of the present invention, the ultrasonic power is 220W.
The Yiqing capsule dregs can be residues obtained by extracting scutellaria baicalensis, coptis chinensis and rheum officinale with water, which are sold in the market.
In a specific embodiment of the invention, the Yiqing capsule dregs are a composition of scutellaria baicalensis dregs, coptis chinensis dregs and rhubarb dregs, and the weight ratio of the scutellaria baicalensis dregs, the coptis chinensis dregs and the rhubarb dregs is 4-6: 8-12: 3-4.
In a specific embodiment of the invention, the weight ratio of the scutellaria baicalensis dregs, the rhubarb dregs and the coptis chinensis dregs is 5: 10: 3.3.
In one embodiment of the invention, the bacteriostatic hand sanitizer further comprises at least one of a surfactant, a humectant, a thickener and an essential oil.
In a specific embodiment of the invention, the antibacterial hand sanitizer comprises the following components in percentage by weight: 10-30% of surfactant, 4-8% of humectant, 1-3% of thickener, 8-15% of antibacterial active ingredient, 0.05-0.2% of mint essential oil and the balance of water. In a specific embodiment of the invention, the antibacterial hand sanitizer comprises the following components in percentage by weight: 20% of surfactant, 6% of humectant, 2% of thickening agent, 12% of bacteriostatic active ingredient, 0.1% of mint essential oil and the balance of water. .
In a specific embodiment of the present invention, the surfactant is at least one of sodium fatty alcohol polyoxyethylene ether sulfate (AES), cocamidopropyl betaine (CAB), lauryl glycoside (APG); the humectant is propylene glycol.
In a specific embodiment of the invention, the surfactant is fatty alcohol-polyoxyethylene ether sodium sulfate, cocamidopropyl betaine and lauryl glycoside, and the weight ratio of the fatty alcohol-polyoxyethylene ether sodium sulfate, the cocamidopropyl betaine and the lauryl glycoside is 5-8: 2-4: 2. In a specific example, the weight ratio of sodium fatty alcohol polyoxyethylene ether sulfate, cocamidopropyl betaine, and lauryl glycoside is 6:3: 2.
The bacteriostatic hand sanitizer can be prepared by the existing method, for example, all the components of the hand sanitizer are mixed and then are uniformly stirred. In one embodiment of the invention, the following method is used for preparation: firstly, adding a surfactant into a beaker, heating, stirring uniformly, adding a humectant, stirring uniformly, then adding water and a thickening agent, and stirring continuously until the solid is completely dissolved. And (3) after the temperature is properly reduced, adding the first clear decoction dreg extracting solution, the eucalyptus extracting solution, the honeysuckle extracting solution and a proper amount of mint essential oil, and stirring uniformly to a constant volume to obtain the antibacterial hand sanitizer.
In a specific embodiment of the invention, the heating is water bath heating, and the temperature is 70-90 ℃, and the preferred temperature is 80 ℃.
In one embodiment of the invention, the water is deionized water. The introduction of impurities can be reduced by using deionized water.
The following examples are provided to further illustrate the embodiments of the present invention and are not intended to limit the scope of the present invention. The ethanol concentrations in the examples are volume concentrations, and the instruments and reagents used in the examples are:
FA200413 ten thousandth electronic balance (shanghai yupinghi scientific instruments ltd); KQ3200E ultrasonic cleaner (kunshan grass ultrasonic instrument ltd); WK-800A high speed drug mill (Chengxi mechanical Co., Ltd., Qingzhou city); YRE-2000B rotary evaporator (Otsuwa instruments, Inc., Cinay); SHB-IIIG circulating water type multipurpose vacuum (Zhengzhou great wall science, industrial and trade, Inc.); UPH-I-20T Ultrafiltration ultrapure water machine (Sichuan Ultrahigh ultrapure technology, Inc.); a GZX-GF101 type electrothermal constant temperature forced air drying oven (Shanghai leap-in medical instruments Co., Ltd.); a vertical pressure steam sterilization pot (Shanghai Bocheng industries, Ltd.) for medical equipment; SHP-160 Biochemical incubator (Tantan blue instruments manufacturing Co., Ltd.); SW-CJ-1D single-person single-face superclean bench.
Staphylococcus aureus is from the Strain preservation Chamber of medical and technology institute of Chengdu Chinese medicine university. Nutrient agar (Beijing Oobozoxin organisms). Absolute ethanol was analytically pure (chengdu conogong chemicals limited). Sodium fatty alcohol polyoxyethylene ether sulfate, cocamidopropyl betaine and lauryl glucoside (all are Shandong Youguo chemical science and technology Co., Ltd.). Propylene glycol was used as an analytical reagent (Shandong Yousio chemical science and technology Co., Ltd.). Thickener 886 and thickener 524 were obtained from Shandong Youso chemical science and technology, Inc., and peppermint essential oil was obtained from Changsha, Mingbang chemical science and technology, Inc. The test water was ultrapure water. The Yiqing capsule dregs are provided by Chengdu Kanghong pharmaceutical industry group member company, and the weight ratio of the scutellaria root dregs, the rhubarb dregs and the coptis root dregs in the dregs is 5: 10: 3.3.
Example 1
The first clear decoction dreg extract is obtained by the following method: weighing dried Yiqing capsule residues, adding 60% ethanol solution, performing ultrasonic extraction with the ultrasonic power of 220W at the extraction temperature of 70 ℃ for 1h at the material-liquid ratio of 1:15g/mL, filtering with gauze to obtain an extracting solution, and performing reduced pressure concentration by using a rotary evaporator (60 ℃ and 160r/min) until the extracting solution is dried to obtain the residue extract.
The eucalyptus leaf extract is obtained by adopting the following method: weighing dried folium Eucalypti Globueli powder, adding 60% ethanol solution, ultrasonic extracting at 70 deg.C for 1h with ultrasonic power of 220W, and material-liquid ratio of 1:15g/mL, filtering with gauze to obtain extractive solution, and concentrating under reduced pressure with rotary evaporator (60 deg.C, 160r/min) to dry to obtain residue extract.
The honeysuckle extract is obtained by adopting the following method: mixing honeysuckle powder and an ethanol solution, refluxing in a water bath, then carrying out solid-liquid separation, and concentrating the liquid to dryness to obtain a honeysuckle extract, wherein the material-liquid ratio of honeysuckle to the ethanol solution is 1:12 g/mL; the concentration of the ethanol solution is 60 percent; the extraction temperature is 50 ℃, and the extraction time is 1 h;
the hand sanitizer comprises the following components: 20% of surfactant (AES: CAB: APG ═ 6:3:2), 6% of humectant (propylene glycol), 2% of thickening agent (thickening agent 886: thickening agent 524 ═ 1:1), 12% of bacteriostatic active ingredient (Yiqing decoction dregs extract 6%, eucalyptus extract 3%, honeysuckle extract 3%), 0.1% of peppermint essential oil and the balance of water.
The preparation method of the hand sanitizer comprises the following steps: based on 100ml of hand sanitizer. Accurately weighing AES, CAB and APG in a 250mL beaker by using a200 mL beaker according to the formula requirement by using a dosing method, heating the beaker in a water bath kettle at 80 ℃, properly stirring the mixture by using a glass rod, adding propylene glycol, stirring the mixture uniformly, adding about 30g of deionized water and a thickening agent (the thickening agent 886: the thickening agent 524 is 1:1), and continuously stirring the mixture until the solid is completely dissolved. And (3) after the temperature is properly reduced, adding the first clear decoction dreg extract, the eucalyptus extract, the honeysuckle extract and the mint essential oil, uniformly stirring, and fixing the volume to obtain the antibacterial hand sanitizer.
The antibacterial effect of the antibacterial hand sanitizer is measured, and the specific method comprises the following steps:
activation of strains: streaking cold-stored Staphylococcus aureus on agar plate, culturing at 37 deg.C for 24 hr for resuscitation, washing with normal saline to obtain bacterial suspension, and performing McLeod to obtain 1 × 106CFU/mL bacterial suspension is prepared just before use.
Determination of the bacteriostatic effect: the bacteriostatic effect was measured by a filter paper plate diffusion method. And sucking 200 mu L of the bacterial suspension to the surface of the completely solidified nutrient agar culture medium by using a micro liquid transfer gun, immediately and uniformly coating by using a sterile glass coating rod, covering a plate, and drying at room temperature for 5-10 min. Placing sterilized filter paper sheets with the diameter of 6mm in a liquid soap with the volume concentration of 50%, soaking for 30min, taking out, drying at 60 ℃, pasting the filter paper sheets on a bacterium-containing flat plate according to a sterile operation, pasting 3 filter paper sheets on each dish of a negative control group which is a treatment solution without an extract solution, placing the filter paper sheets in an incubator with the temperature of 37 ℃ for culturing for 18-24 h, measuring the size of a bacteriostatic circle, and taking an average value.
As a result: the diameter of the inhibition zone is 16.5 mm.
Comparative example 1 (Yiqing original medicine hand cleanser)
Referring to example 1, the first clear technical extract was used to prepare a bacteriostatic hand sanitizer.
The first clear active compound extract is obtained by the following method: weighing 10g of dried Yiqing capsule compound medicinal material (scutellaria baicalensis: rheum officinale: coptis chinensis: 5: 10: 3.3), adding 60% ethanol solution by volume concentration, carrying out ultrasonic extraction at the ultrasonic power of 220W and the extraction temperature of 70 ℃, extracting for 1h at the material-liquid ratio of 1:15g/mL, filtering with gauze after extraction to obtain an extracting solution, and carrying out reduced pressure concentration on the extracting solution by using a rotary evaporator (60 ℃ and 160r/min) until the extracting solution is dried to obtain the Yiqing original medicinal material extract.
The components and the preparation method of the hand sanitizer are the same as those in example 1, and only the first clear decoction dreg extract is replaced by the first clear original medicine extract, so that the antibacterial hand sanitizer is obtained.
The bacteriostatic activity of the bacteriostatic hand sanitizer against staphylococcus aureus was measured by the method described in example 1, and the results are shown in table 1.
Comparative example 2 (hand cleanser without bacteriostatic active ingredient, negative control)
The hand sanitizer comprises the following components: 20% of surfactant (AES: CAB: APG ═ 6:3:2), 6% of humectant (propylene glycol), 2% of thickener (thickener 886: thickener 524 ═ 1:1), 0.1% of peppermint essential oil and the balance of water.
The preparation method of the hand sanitizer comprises the following steps: based on 100ml of hand sanitizer. Accurately weighing AES, CAB and APG in a200 mL beaker by a dosing method according to the formula requirement, heating in a water bath kettle at 80 ℃, properly stirring by using a glass rod, adding propylene glycol, stirring uniformly, adding about 30g of deionized water and a thickening agent, and continuously stirring until the solid is completely dissolved. And (3) after the temperature is properly reduced, adding the peppermint essential oil, uniformly stirring, and fixing the volume to obtain the hand sanitizer.
The bacteriostatic activity of the hand sanitizer against staphylococcus aureus was measured by the method described in example 1, and the results are shown in table 1.
Comparative example 3 (bacteriostatic hand sanitizer lacking the first cleaning residue extract)
The hand sanitizer comprises the following components: 20% of surfactant (AES: CAB: APG ═ 6:3:2), 6% of humectant (propylene glycol), 2% of thickening agent (thickening agent 886: thickening agent 524 ═ 1:1), 12% of bacteriostatic active ingredient (eucalyptus extract, 6% of honeysuckle extract), 0.1% of peppermint essential oil and the balance of water.
The preparation method of the hand sanitizer comprises the following steps: based on 100ml of hand sanitizer. Accurately weighing AES, CAB and APG in a200 mL beaker by a dosing method according to the formula requirement, heating in a water bath kettle at 80 ℃, properly stirring by using a glass rod, adding propylene glycol, stirring uniformly, adding about 30g of deionized water and a thickening agent, and continuously stirring until the solid is completely dissolved. And (3) after the temperature is properly reduced, adding the eucalyptus extract, the honeysuckle extract and the peppermint essential oil, uniformly stirring, and fixing the volume to obtain the hand sanitizer.
The bacteriostatic activity of the hand sanitizer against staphylococcus aureus was measured by the method described in example 1, and the results are shown in table 1.
Comparative example 4 (Positive control of current bacteriostatic hand sanitizer)
The bacteriostatic activity of the currently commercially available bacteriostatic hand sanitizer (blue moon bacteriostatic hand sanitizer) against staphylococcus aureus was measured by the method described in example 1, and the results are shown in table 1.
Comparative example 5 (changing the method for extracting the first clear residue, the rest is unchanged)
Referring to example 1, a liquid soap was prepared by changing the extraction method of first clear residue.
The first clear decoction dreg extract is obtained by the following method (ethanol reflux method is adopted to recover the decoction dregs): weighing 10g of dried Yiqing capsule residues, placing into a conical flask with a plug, adding 100mL of ethanol, refluxing in water bath for 1h, filtering with gauze to obtain an extracting solution, and concentrating under reduced pressure by using a rotary evaporator (60 ℃, 160r/min) until the extracting solution is dried to obtain the Yiqing capsule residue extract.
The components and the preparation method of the hand sanitizer are the same as those of example 1, so that the antibacterial hand sanitizer is obtained.
The bacteriostatic activity of the bacteriostatic hand sanitizer against staphylococcus aureus was measured by the method described in example 1, and the results are shown in table 1.
TABLE 1
| Numbering | Diameter of bacteriostatic circle (mm) |
| Example 1 | 16.5 |
| Comparative example 1 | 17.5 |
| Comparative example 2 | 8.0 |
| Comparative example 3 | 11.0 |
| Comparative example 4 | 17.0 |
| Comparative example 5 | 11.5 |
Therefore, the antibacterial hand sanitizer is prepared by adopting the first-cleaning decoction dreg extract, the first-cleaning decoction dreg extract can be matched with the eucalyptus leaf extract and the honeysuckle extract, and the obtained hand sanitizer has a good antibacterial effect, even has the antibacterial effect equivalent to that of a raw medicinal material extract, and is similar to that of the existing antibacterial hand sanitizer.
Example 2
Referring to the method of example 1, the formulation of the hand sanitizer was changed to obtain a bacteriostatic hand sanitizer.
The hand sanitizer comprises the following components: 20% of surfactant (AES: CAB: APG ═ 6:3:2), 6% of humectant (propylene glycol), 2% of thickening agent (thickening agent 886: thickening agent 524 ═ 1:1), 12% of bacteriostatic active ingredient (Yiqing decoction dregs extract, 4% of eucalyptus extract, 4% of honeysuckle extract), 0.1% of peppermint essential oil and the balance of water.
The preparation method is the same as that of example 1, and the antibacterial hand sanitizer is obtained.
The bacteriostatic activity of the bacteriostatic hand sanitizer on staphylococcus aureus is determined by the method described in example 1, and the diameter of a bacteriostatic zone is 14.0 mm.
Claims (10)
1. Antibacterial liquid soap, its characterized in that: the antibacterial hand sanitizer contains 8-15 wt% of antibacterial active ingredients, wherein the antibacterial active ingredients comprise a first clear decoction dreg extract, a eucalyptus leaf extract and a honeysuckle extract, and the weight ratio of the first clear decoction dreg extract to the eucalyptus leaf extract to the honeysuckle extract is 1-3: 0.5-1.5: 1;
wherein the first clear decoction dreg extract is obtained by adopting the following method: mixing the Yiqing capsule residues with an ethanol solution, carrying out ultrasonic extraction, carrying out solid-liquid separation, and concentrating the liquid to dryness to obtain a Yiqing capsule residue extract, wherein the feed-liquid ratio of the Yiqing capsule residues to the ethanol solution is 1: 10-25 g/mL; the volume concentration of the ethanol solution is 50-60%; the extraction temperature is 60-80 ℃, and the extraction time is 1-2 h;
the eucalyptus leaf extract is obtained by adopting the following method: mixing the eucalyptus leaves with an ethanol solution, carrying out ultrasonic extraction, carrying out solid-liquid separation, and concentrating the liquid to dryness to obtain a eucalyptus leaf extract, wherein the material-liquid ratio of the eucalyptus leaves to the ethanol solution is 1: 8-12 g/mL; the volume concentration of the ethanol solution is 55-65%; the ultrasonic temperature is 55-65 ℃, and the ultrasonic time is 0.8-1.2 h;
the honeysuckle extract is obtained by adopting the following method: mixing honeysuckle with an ethanol solution, refluxing in a water bath, then carrying out solid-liquid separation, and concentrating the liquid to dryness to obtain a honeysuckle extract, wherein the material-liquid ratio of the honeysuckle to the ethanol solution is 1: 10-14 g/mL; the volume concentration of the ethanol solution is 55-65%; the extraction temperature is 45-55 ℃, and the reflux time is 3-5 h.
2. The bacteriostatic hand sanitizer according to claim 1, which is characterized in that: the weight ratio of the first clear decoction dreg extract to the eucalyptus leaf extract to the honeysuckle extract is 2: 1.
3. The bacteriostatic hand sanitizer according to claim 1, which is characterized in that: the bacteriostatic hand sanitizer contains 12 wt% of bacteriostatic active ingredients.
4. The bacteriostatic hand sanitizer according to claim 1, which is characterized in that: when the Yiqing decoction dreg extract is prepared, the material-liquid ratio of the Yiqing capsule decoction dregs to the ethanol solution is 1:15 g/mL; the volume concentration of the ethanol solution is 60 percent; the extraction temperature is 70 ℃, and the extraction time is 1 h;
when the eucalyptus leaf extract is prepared, the feed-liquid ratio of the eucalyptus leaves to the ethanol solution is 1: 10 g/mL; the volume concentration of the ethanol solution is 60 percent; the ultrasonic temperature is 60 ℃, and the ultrasonic time is 1 h;
when the honeysuckle extract is prepared, the feed-liquid ratio of the honeysuckle to the ethanol solution is 1:12 g/mL; the volume concentration of the ethanol solution is 60 percent; the extraction temperature is 50 ℃, and the reflux time is 4 h.
5. The bacteriostatic hand sanitizer according to claim 1, which is characterized in that: the ultrasonic power is 200-250W; preferably the ultrasonic power is 220W.
6. The bacteriostatic hand sanitizer according to claim 1, which is characterized in that: the Yiqing capsule dregs are residues of water extraction of scutellaria baicalensis, coptis chinensis and rheum officinale; preferably, the Yiqing capsule dregs are a composition of scutellaria baicalensis dregs, coptis chinensis dregs and rhubarb dregs, and the weight ratio of the scutellaria baicalensis dregs, the coptis chinensis dregs and the rhubarb dregs is 4-6: 8-12: 3-4; preferably, the weight ratio of the scutellaria residue, the rhubarb residue and the coptis residue is 5: 10: 3.3.
7. The bacteriostatic hand sanitizer according to claim 1, which is characterized in that: the antibacterial hand sanitizer also comprises at least one of a surfactant, a humectant, a thickener and essential oil.
8. The bacteriostatic hand sanitizer according to claim 6, which is characterized in that: the antibacterial hand sanitizer comprises the following components in percentage by weight: 10-30% of a surfactant, 4-8% of a humectant, 1-3% of a thickening agent, 8-15% of an antibacterial active ingredient, 0.05-0.2% of mint essential oil and the balance of water; preferably, the antibacterial hand sanitizer comprises the following components in percentage by weight: 20% of surfactant, 6% of humectant, 2% of thickening agent, 12% of bacteriostatic active ingredient, 0.1% of mint essential oil and the balance of water.
9. The bacteriostatic hand sanitizer according to claim 8, which is characterized in that: the surfactant is at least one of fatty alcohol-polyoxyethylene ether sodium sulfate, cocamidopropyl betaine and lauryl glucoside; the humectant is propylene glycol.
10. The bacteriostatic hand sanitizer according to claim 9, which is characterized in that: the surfactant is fatty alcohol-polyoxyethylene ether sodium sulfate, cocamidopropyl betaine and lauryl glucoside, and the weight ratio of the fatty alcohol-polyoxyethylene ether sodium sulfate, the cocamidopropyl betaine and the lauryl glucoside is 5-8: 2-4: 2; preferably, the weight ratio of the sodium fatty alcohol polyoxyethylene ether sulfate, the cocamidopropyl betaine and the lauryl glucoside is 6:3: 2.
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| CN202110587197.3A CN113599313A (en) | 2021-05-27 | 2021-05-27 | Antibacterial hand sanitizer |
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Application publication date: 20211105 |