CN113576964A - Plant extraction cell repair method - Google Patents
Plant extraction cell repair method Download PDFInfo
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- CN113576964A CN113576964A CN202110841305.5A CN202110841305A CN113576964A CN 113576964 A CN113576964 A CN 113576964A CN 202110841305 A CN202110841305 A CN 202110841305A CN 113576964 A CN113576964 A CN 113576964A
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- 238000000605 extraction Methods 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 31
- 230000008439 repair process Effects 0.000 title claims abstract description 7
- 239000000284 extract Substances 0.000 claims abstract description 49
- 241000209140 Triticum Species 0.000 claims abstract description 47
- 235000021307 Triticum Nutrition 0.000 claims abstract description 47
- 235000015742 Nephelium litchi Nutrition 0.000 claims abstract description 33
- 241000196324 Embryophyta Species 0.000 claims abstract description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 19
- 239000000341 volatile oil Substances 0.000 claims abstract description 13
- 239000000419 plant extract Substances 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 6
- 239000003906 humectant Substances 0.000 claims abstract description 6
- 239000003755 preservative agent Substances 0.000 claims abstract description 6
- 230000002335 preservative effect Effects 0.000 claims abstract description 6
- 239000002904 solvent Substances 0.000 claims abstract description 6
- 239000002562 thickening agent Substances 0.000 claims abstract description 6
- 239000008367 deionised water Substances 0.000 claims abstract description 5
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 5
- 244000183278 Nephelium litchi Species 0.000 claims description 33
- 238000002360 preparation method Methods 0.000 claims description 28
- 235000004879 dioscorea Nutrition 0.000 claims description 19
- 239000000843 powder Substances 0.000 claims description 12
- 239000004365 Protease Substances 0.000 claims description 10
- 235000002722 Dioscorea batatas Nutrition 0.000 claims description 8
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- 240000001811 Dioscorea oppositifolia Species 0.000 claims description 8
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- 229940088598 enzyme Drugs 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 7
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- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 6
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- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims description 4
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 claims description 4
- 108090000145 Bacillolysin Proteins 0.000 claims description 4
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- 229920002674 hyaluronan Polymers 0.000 claims description 4
- 229960003160 hyaluronic acid Drugs 0.000 claims description 4
- QELSKZZBTMNZEB-UHFFFAOYSA-N propylparaben Chemical compound CCCOC(=O)C1=CC=C(O)C=C1 QELSKZZBTMNZEB-UHFFFAOYSA-N 0.000 claims description 4
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- PRAKJMSDJKAYCZ-UHFFFAOYSA-N squalane Chemical compound CC(C)CCCC(C)CCCC(C)CCCCC(C)CCCC(C)CCCC(C)C PRAKJMSDJKAYCZ-UHFFFAOYSA-N 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 108090000526 Papain Proteins 0.000 claims description 3
- 229930003427 Vitamin E Natural products 0.000 claims description 3
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- 238000001035 drying Methods 0.000 claims description 3
- 230000001804 emulsifying effect Effects 0.000 claims description 3
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- 238000001914 filtration Methods 0.000 claims description 3
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 3
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- 235000019834 papain Nutrition 0.000 claims description 3
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- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 2
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 2
- 239000004475 Arginine Substances 0.000 claims description 2
- 102000008186 Collagen Human genes 0.000 claims description 2
- 108010035532 Collagen Proteins 0.000 claims description 2
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 2
- 229920002125 Sokalan® Polymers 0.000 claims description 2
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 2
- 229960001631 carbomer Drugs 0.000 claims description 2
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- 239000004359 castor oil Substances 0.000 claims description 2
- 235000019438 castor oil Nutrition 0.000 claims description 2
- 229960005188 collagen Drugs 0.000 claims description 2
- 229920001436 collagen Polymers 0.000 claims description 2
- 229960005150 glycerol Drugs 0.000 claims description 2
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 claims description 2
- 229920003063 hydroxymethyl cellulose Polymers 0.000 claims description 2
- 229940031574 hydroxymethyl cellulose Drugs 0.000 claims description 2
- -1 jojoba oil Chemical compound 0.000 claims description 2
- 229940119170 jojoba wax Drugs 0.000 claims description 2
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 claims description 2
- JXTPJDDICSTXJX-UHFFFAOYSA-N n-Triacontane Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC JXTPJDDICSTXJX-UHFFFAOYSA-N 0.000 claims description 2
- 239000000661 sodium alginate Substances 0.000 claims description 2
- 235000010413 sodium alginate Nutrition 0.000 claims description 2
- 229940005550 sodium alginate Drugs 0.000 claims description 2
- 229940032094 squalane Drugs 0.000 claims description 2
- 239000000230 xanthan gum Substances 0.000 claims description 2
- 235000010493 xanthan gum Nutrition 0.000 claims description 2
- 229920001285 xanthan gum Polymers 0.000 claims description 2
- 229940082509 xanthan gum Drugs 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims 1
- 210000003491 skin Anatomy 0.000 abstract description 20
- 210000004027 cell Anatomy 0.000 abstract description 13
- 241000894006 Bacteria Species 0.000 abstract description 3
- 206010061218 Inflammation Diseases 0.000 abstract description 3
- 210000001339 epidermal cell Anatomy 0.000 abstract description 3
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- 241001629511 Litchi Species 0.000 description 6
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- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
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- 238000010298 pulverizing process Methods 0.000 description 2
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- 108010004032 Bromelains Proteins 0.000 description 1
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- 239000008346 aqueous phase Substances 0.000 description 1
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- 230000009286 beneficial effect Effects 0.000 description 1
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- 239000002537 cosmetic Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Chemical & Material Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Pain & Pain Management (AREA)
- Rheumatology (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a plant extraction cell repairing method, which comprises the following steps: applying the plant extract essence on skin; the plant extraction essence comprises the following raw materials in percentage by weight: 2-6% of yam peptide extract; 2-4% of lychee seed extract; 3-6% of wheat seedling essential oil; vitamin E1-3%; solubilizer: 3 to 5 percent; humectant: 10-20%; 0.1 to 0.5 percent of emulsifier; 0.05 to 0.1 percent of thickening agent; 0.01 to 0.05 percent of preservative; 0.01 to 0.05 percent of pH regulator; the balance of deionized water. The invention can resist bacteria and diminish inflammation, stimulate epidermal cells to grow continuously, repair skin cells, and play a role in removing pockmarks and restoring skin elasticity.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to a plant extraction cell repairing method.
Background
With the increase of age and working pressure, the skin gradually becomes old, and skin problems such as dryness, fine lines, dullness, melanin deposition and the like follow up, and serious skin problems such as acne, pimples and the like easily occur in severe people. For this reason, various skin care products are available on the market, but the main problems are: 1) the chemical substance is a main functional component, and the chemical substance plays a role in repairing cells and simultaneously is accompanied with the deposition of heavy metal ions, so that the dependence is often formed; 2) in order to eliminate acne and pimples, some skin care products are also added with bactericides such as antibiotics and the like, so that the long-term use of the skin care products not only has high rebound rate, but also is easy to generate drug resistance; therefore, it is necessary to develop a skin care lotion containing plant components as effective components, and to cooperate with a cell repair method to perform cell repair on aged cells, improve skin elasticity, reduce wrinkle generation, prevent acne and pimple generation, and make skin cells full of vitality.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a plant extract cell repairing method which can resist bacteria and diminish inflammation, stimulate epidermal cells to grow continuously, repair skin cells and play roles in removing pockmarks and restoring skin elasticity.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a plant extraction cell repairing method comprises the following steps: applying the plant extract essence to the skin;
the plant extraction essence comprises the following raw materials in percentage by weight:
further, the humectant is selected from one or more of squalane, sodium alginate, jojoba oil, hyaluronic acid, collagen, hyaluronic acid and glycerol;
the emulsifier is one or more selected from glycerol stearate, polyglycerol-3 diisostearate, polyglycerol-6 distearate and polyglycerol-6 dioleate;
the thickening agent is selected from one or more of carbomer, xanthan gum and hydroxymethyl cellulose;
the solubilizer adopts PEG-40 hydrogenated castor oil;
the preservative is selected from one or more of methyl hydroxybenzoate, propyl hydroxybenzoate and propyl hydroxybenzoate;
the pH regulator adopts one or two of triethanolamine and arginine.
Further, the preparation method of the lychee seed extract comprises the following steps:
firstly, removing impurities from lychee seeds, rinsing, drying, crushing by a traditional Chinese medicine crusher, and then sieving by a 50-80-mesh sieve to obtain lychee seed powder;
then, extracting the lychee seed powder by using a supercritical CO2 extraction instrument to obtain a lychee seed extract; the setting parameters of the supercritical CO2 extraction instrument are as follows: the extraction kettle pressure is 20MPa, the extraction temperature is 40 ℃, the separation kettle pressure is 5MPa, the temperature is 45 ℃, the CO2 flow is 13L/h, and the extraction time is 120 min.
Further, the preparation method of the wheat seedling extract comprises the following steps:
firstly, cutting wheat seedlings in a growing period, wherein the height of the overground part of the wheat seedlings is 10-20cm, and then washing the wheat seedlings by water and draining the wheat seedlings;
then, cutting the wheat seedlings and mechanically extruding, and removing juice to obtain wheat seedling solid matters;
and finally, distilling the extruded solid matter, and collecting distillate, namely the wheat seedling essential oil.
Further, the preparation method of the yam peptide extract comprises the following steps:
firstly, crushing and pulping fresh Chinese yam, adding 2 times of water by volume, heating to boil, keeping for 10min, and cooling to obtain Chinese yam pulp; the rhizoma Dioscoreae is rhizoma Dioscoreae;
and then, adjusting the pH value of the yam pulp to 5.5-6.5, adding compound protease, carrying out enzymolysis for 5 hours at 50-60 ℃, heating to 80-90 ℃, inactivating enzyme, centrifuging, filtering with a membrane, concentrating and carrying out spray drying to obtain the yam peptide extract.
Furthermore, the compound protease is prepared by compounding papain and neutral protease according to the ratio of 1: 0.5.
Further, the preparation method of the plant extraction essence comprises the following steps:
step 1, mixing the humectant, the thickener, the yam peptide extract and deionized water, and stirring at 65-70 ℃ for 15-20min to obtain a water phase;
step 2, mixing the solubilizer, the lychee seed extract, the wheat seedling essential oil, the vitamin E, the emulsifier and the preservative, and uniformly stirring to obtain an oil phase;
and 3, dropwise adding the oil phase into the water phase at the rotating speed of 10000 revolutions per minute, and fully emulsifying to obtain the emulsion.
Further, the plant extract essence is smeared on the skin and then is gently massaged until being absorbed.
Compared with the prior art, the invention has the following beneficial effects:
the formula disclosed by the invention is scientific in matching, natural and safe, has no side effect, and the Chinese yam peptide extract, the lychee seed extract and the wheat seedling essential oil are synergistic in effect and play a role together, so that the effects of resisting bacteria and diminishing inflammation, stimulating the continuous growth of epidermal cells, repairing the cells and achieving the purposes of removing pockmarks and recovering the elasticity of skin are achieved.
Detailed Description
Example 1
A plant extraction cell repairing method comprises the following steps: applying the plant extract essence on skin; then gently massage until absorption.
The plant extraction essence comprises the following raw materials in percentage by weight:
deionized water: and (4) the balance.
The preparation method of the lychee seed extract comprises the following steps:
firstly, removing impurities from lychee seeds, rinsing, drying, crushing by a traditional Chinese medicine crusher, and then sieving by a 50-80-mesh sieve to obtain lychee seed powder;
then, supercritical CO is adopted for the lychee seed powder2Extracting with an extractorObtaining lychee seed extract; supercritical CO2The setting parameters of the extraction instrument are as follows: the pressure of the extraction kettle is 20MPa, the extraction temperature is 40 ℃, the pressure of the separation kettle is 5MPa, the temperature is 45 ℃, and CO is generated2The flow rate is 13L/h, and the extraction time is 120 min.
The preparation method of the wheat seedling extract comprises the following steps:
firstly, cutting wheat seedlings in a growing period, wherein the height of the overground part of the wheat seedlings is 10-20cm, and then washing the wheat seedlings by water and draining the wheat seedlings;
then, cutting the wheat seedlings and mechanically extruding, and removing juice to obtain wheat seedling solid matters;
and finally, distilling the extruded solid matter, and collecting distillate, namely the wheat seedling essential oil.
The preparation method of the Chinese yam peptide extract comprises the following steps:
firstly, crushing and pulping fresh Chinese yam, adding 2 times of water by volume, heating to boil, keeping for 10min, and cooling to obtain Chinese yam pulp;
and then, adjusting the pH value of the yam pulp to 5.5-6.5, adding compound protease, carrying out enzymolysis for 5 hours at 50-60 ℃, heating to 80-90 ℃, inactivating enzyme, centrifuging, filtering with a membrane, concentrating and carrying out spray drying to obtain the yam peptide extract.
The compound protease is prepared by compounding papain and neutral protease according to the ratio of 1: 0.5.
The preparation method of the plant extraction essence comprises the following steps:
step 1, mixing the humectant, the thickener, the yam peptide extract and deionized water, and stirring at 65-70 ℃ for 15-20min to obtain a water phase;
step 2, mixing the solubilizer, the lychee seed extract, the wheat seedling essential oil, the vitamin E, the emulsifier and the preservative, and uniformly stirring to obtain an oil phase;
and 3, dropwise adding the oil phase into the water phase at the rotating speed of 10000 revolutions per minute, and fully emulsifying to obtain the emulsion.
Example 2
A plant extraction cell repairing method comprises the following steps: applying the plant extract essence on skin; then gently massage until absorption.
The plant extraction essence comprises the following raw materials in percentage by weight:
the preparation method of the lychee seed extract is the same as that in the example 1;
wherein, the preparation method of the wheat seedling extract is the same as that of the example 1;
wherein, the preparation method of the yam peptide extract is the same as that of the embodiment 1;
the preparation method of the plant extraction essence is the same as that of the embodiment 1;
example 3
A plant extraction cell repairing method comprises the following steps: applying the plant extract essence on skin; then gently massage until absorption.
The plant extraction essence comprises the following raw materials in percentage by weight:
the preparation method of the lychee seed extract is the same as that in the example 1;
wherein, the preparation method of the wheat seedling extract is the same as that of the example 1;
wherein, the preparation method of the yam peptide extract is the same as that of the embodiment 1;
the preparation method of the plant extraction essence is the same as that of the embodiment 1;
comparative example 1:
the same as example 1, except for the preparation method of the lychee seed extract,
in this comparative example, the preparation method of the lychee seed extract comprises the following steps:
removing impurities from semen litchi, rinsing, oven drying, pulverizing with a traditional Chinese medicine pulverizer, and sieving with 50-80 mesh sieve to obtain semen litchi powder;
then, according to the mass ratio of the lychee seed powder to the liquid water of 1:8, adding water into the lychee seed powder, distilling, and collecting distillate, namely the lychee seed extract.
Comparative example 2:
the same as example 1, except for the preparation method of the lychee seed extract,
in this comparative example, the preparation method of the lychee seed extract comprises the following steps:
removing impurities from semen litchi, rinsing, oven drying, pulverizing with a traditional Chinese medicine pulverizer, and sieving with 50-80 mesh sieve to obtain semen litchi powder;
then, according to the mass ratio of the lychee seed powder to the water liquid of 1:8, adding water into the lychee seed powder, decocting for 2 times with slow fire, combining the decoction of the two times, concentrating, and performing spray drying to obtain the lychee seed extract.
Comparative example 3
The same as example 1, except that the litchi seed extract was not added to the raw material.
Comparative example 4:
the same as example 1, except that the complex enzyme used in the preparation process of the yam peptide extract is different.
In the comparative example, the complex enzyme used in the preparation process of the yam peptide extract is prepared from bromelain and neutral protease according to the ratio of 1: 0.5.
Comparative example 5
The same as example 1, except that the yam peptide extract was not added to the raw material.
Comparative example 6
The same as in example 1, except that not the essential oil of wheat seedlings but the extract of wheat seedlings was used, the plant extract essence was prepared by adding the extract of wheat seedlings in an aqueous phase, not an oil phase.
The preparation method of the wheat seedling extract comprises the following steps:
firstly, cutting wheat seedlings in a growing period, wherein the height of the overground part of the wheat seedlings is 10-20cm, and then washing the wheat seedlings by water and draining the wheat seedlings;
then, cutting the wheat seedlings into pieces, pulping, and collecting juice to obtain wheat seedling juice;
finally, heating the collected wheat seedling juice to inactivate enzyme, concentrating under reduced pressure, and freeze drying to obtain wheat seedling extract.
Comparative example 7
The procedure was as in example 1 except that no wheat seedling essential oil was added to the starting materials.
Effect example 1:
selecting 400 volunteers with pockmarks on the face, wherein the volunteers are 15-45 years old, 25 years old and 200 women in each year; randomly divided into 8 groups of 50 people each;
one group was tested and the essence and the application method described in example 1 were used to care skin;
comparing one group, and caring skin by adopting the essence and the using method recorded in the comparative example 1;
comparing two groups, and caring skin by adopting the essence and the using method recorded in the comparative example 2;
comparing the three groups, and caring skin by adopting the essence and the using method recorded in the comparative example 3;
comparing the four groups, and caring skin by adopting the essence and the using method recorded in the comparative example 4;
comparing the five groups, and caring skin by adopting the essence and the using method recorded in the comparative example 5;
comparing the six groups, and caring skin by adopting the essence and the using method recorded in the comparative example 6;
comparing seven groups, and caring skin by adopting the essence and the using method recorded in the comparative example 7;
the using method is carried out twice a day for 2 months continuously, the using effect is observed, and the result is shown in the following table.
The effect of use was observed according to the essence and the method of use described in each example and comparative example, twice daily for 2 months.
The curative effect is as follows: percent efficacy ═ 100% treatment-pre-treatment pock count/post-treatment pock count;
the effect is shown: pockmark subsides by 60-90%, pockmark is relieved;
the method has the following advantages: pockmark subsides by 30-60%, pockmark improves;
and (4) invalidation: pockmark regression was less than 30%, pockmark was not improved;
show effect% | Effective% | Not effective% | |
Compare a group | 92 | 6 | 2 |
Test A set | 16 | 60 | 24 |
Two groups of experiments | 12 | 66 | 22 |
Three groups of experiments | 8 | 56 | 46 |
Test four groups | 22 | 64 | 14 |
Test five groups | 14 | 58 | 28 |
Six groups of experiments | 10 | 72 | 18 |
Test seven groups | 6 | 60 | 44 |
As can be seen from the data in the table, the removal effect of any one of the yam peptide extract, the lychee seed extract and the wheat seedling essential oil on pockmarks is reduced sharply, so that: the yam peptide extract, the lychee seed extract and the wheat seedling essential oil are coordinated to achieve the purpose of removing pockmarks. In addition, the data in the table can also show that the types of enzymes used in the yam peptide extraction process, the litchi seed extraction method and the preparation method of the wheat seedling essential oil play a crucial role in the synergistic acne removing effect.
The embodiments described above are only preferred embodiments of the invention and are not exhaustive of the possible implementations of the invention. Any obvious modifications to the above would be obvious to those of ordinary skill in the art, but would not bring the invention so modified beyond the spirit and scope of the present invention.
Claims (8)
2. the method of repairing plant extractive cells as claimed in claim 1,
the humectant is selected from one or more of squalane, sodium alginate, jojoba oil, hyaluronic acid, collagen, hyaluronic acid and glycerol;
the emulsifier is one or more selected from glycerol stearate, polyglycerol-3 diisostearate, polyglycerol-6 distearate and polyglycerol-6 dioleate;
the thickening agent is selected from one or more of carbomer, xanthan gum and hydroxymethyl cellulose;
the solubilizer adopts PEG-40 hydrogenated castor oil;
the preservative is selected from one or more of methyl hydroxybenzoate, propyl hydroxybenzoate and propyl hydroxybenzoate;
the pH regulator adopts one or two of triethanolamine and arginine.
3. The method of repairing plant extractive cells as claimed in claim 1,
the preparation method of the lychee seed extract comprises the following steps:
firstly, removing impurities from lychee seeds, rinsing, drying, crushing by a traditional Chinese medicine crusher, and then sieving by a 50-80-mesh sieve to obtain lychee seed powder;
then, supercritical CO is adopted for the lychee seed powder2Extracting with an extractor to obtain semen litchi extract; supercritical CO2The setting parameters of the extraction instrument are as follows: extraction kettle pressure of 20MPa, extractTaking the mixture at the temperature of 40 ℃, the pressure of a separation kettle at the temperature of 45 ℃ and CO at the temperature of 5MPa2The flow rate is 13L/h, and the extraction time is 120 min.
4. The method of repairing plant extractive cells as claimed in claim 1,
the preparation method of the wheat seedling extract comprises the following steps:
firstly, cutting wheat seedlings in a growing period, wherein the height of the overground part of the wheat seedlings is 10-20cm, and then washing the wheat seedlings by water and draining the wheat seedlings;
then, cutting the wheat seedlings and mechanically extruding, and removing juice to obtain wheat seedling solid matters;
and finally, distilling the extruded solid matter, and collecting distillate, namely the wheat seedling essential oil.
5. The method of repairing plant extractive cells as claimed in claim 1,
the preparation method of the yam peptide extract comprises the following steps:
firstly, crushing and pulping fresh Chinese yam, adding 2 times of water by volume, heating to boil, keeping for 10min, and cooling to obtain Chinese yam pulp;
and then, adjusting the pH value of the yam pulp to 5.5-6.5, adding compound protease, carrying out enzymolysis for 5 hours at 50-60 ℃, heating to 80-90 ℃, inactivating enzyme, centrifuging, filtering with a membrane, concentrating and carrying out spray drying to obtain the yam peptide extract.
6. The method of claim 5, wherein the plant extract cell repair is performed by a method comprising,
the compound protease is prepared by compounding papain and neutral protease according to the ratio of 1: 0.5.
7. The method of repairing plant extractive cells as claimed in claim 1,
the preparation method of the plant extraction essence comprises the following steps:
step 1, mixing the humectant, the thickener, the yam peptide extract and deionized water, and stirring at 65-70 ℃ for 15-20min to obtain a water phase;
step 2, mixing the solubilizer, the lychee seed extract, the wheat seedling essential oil, the vitamin E, the emulsifier and the preservative, and uniformly stirring to obtain an oil phase;
and 3, dropwise adding the oil phase into the water phase at the rotating speed of 10000 revolutions per minute, and fully emulsifying to obtain the emulsion.
8. The method for repairing plant-derived cells according to claim 1, wherein the plant-derived essence is applied to the skin and gently massaged until absorbed.
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