CN113576964A - Plant extraction cell repair method - Google Patents

Plant extraction cell repair method Download PDF

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CN113576964A
CN113576964A CN202110841305.5A CN202110841305A CN113576964A CN 113576964 A CN113576964 A CN 113576964A CN 202110841305 A CN202110841305 A CN 202110841305A CN 113576964 A CN113576964 A CN 113576964A
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plant
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cells
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房小华
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/645Proteins of vegetable origin; Derivatives or degradation products thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67Vitamins
    • A61K8/678Tocopherol, i.e. vitamin E
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/92Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A61K8/922Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9789Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00Drugs for dermatological disorders
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005Antimicrobial preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/805Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95

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  • Cosmetics (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention relates to a plant extraction cell repairing method, which comprises the following steps: applying the plant extract essence on skin; the plant extraction essence comprises the following raw materials in percentage by weight: 2-6% of yam peptide extract; 2-4% of lychee seed extract; 3-6% of wheat seedling essential oil; vitamin E1-3%; solubilizer: 3 to 5 percent; humectant: 10-20%; 0.1 to 0.5 percent of emulsifier; 0.05 to 0.1 percent of thickening agent; 0.01 to 0.05 percent of preservative; 0.01 to 0.05 percent of pH regulator; the balance of deionized water. The invention can resist bacteria and diminish inflammation, stimulate epidermal cells to grow continuously, repair skin cells, and play a role in removing pockmarks and restoring skin elasticity.

Description

Plant extraction cell repair method
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to a plant extraction cell repairing method.
Background
With the increase of age and working pressure, the skin gradually becomes old, and skin problems such as dryness, fine lines, dullness, melanin deposition and the like follow up, and serious skin problems such as acne, pimples and the like easily occur in severe people. For this reason, various skin care products are available on the market, but the main problems are: 1) the chemical substance is a main functional component, and the chemical substance plays a role in repairing cells and simultaneously is accompanied with the deposition of heavy metal ions, so that the dependence is often formed; 2) in order to eliminate acne and pimples, some skin care products are also added with bactericides such as antibiotics and the like, so that the long-term use of the skin care products not only has high rebound rate, but also is easy to generate drug resistance; therefore, it is necessary to develop a skin care lotion containing plant components as effective components, and to cooperate with a cell repair method to perform cell repair on aged cells, improve skin elasticity, reduce wrinkle generation, prevent acne and pimple generation, and make skin cells full of vitality.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a plant extract cell repairing method which can resist bacteria and diminish inflammation, stimulate epidermal cells to grow continuously, repair skin cells and play roles in removing pockmarks and restoring skin elasticity.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a plant extraction cell repairing method comprises the following steps: applying the plant extract essence to the skin;
the plant extraction essence comprises the following raw materials in percentage by weight:
Figure BDA0003178931670000011
further, the humectant is selected from one or more of squalane, sodium alginate, jojoba oil, hyaluronic acid, collagen, hyaluronic acid and glycerol;
the emulsifier is one or more selected from glycerol stearate, polyglycerol-3 diisostearate, polyglycerol-6 distearate and polyglycerol-6 dioleate;
the thickening agent is selected from one or more of carbomer, xanthan gum and hydroxymethyl cellulose;
the solubilizer adopts PEG-40 hydrogenated castor oil;
the preservative is selected from one or more of methyl hydroxybenzoate, propyl hydroxybenzoate and propyl hydroxybenzoate;
the pH regulator adopts one or two of triethanolamine and arginine.
Further, the preparation method of the lychee seed extract comprises the following steps:
firstly, removing impurities from lychee seeds, rinsing, drying, crushing by a traditional Chinese medicine crusher, and then sieving by a 50-80-mesh sieve to obtain lychee seed powder;
then, extracting the lychee seed powder by using a supercritical CO2 extraction instrument to obtain a lychee seed extract; the setting parameters of the supercritical CO2 extraction instrument are as follows: the extraction kettle pressure is 20MPa, the extraction temperature is 40 ℃, the separation kettle pressure is 5MPa, the temperature is 45 ℃, the CO2 flow is 13L/h, and the extraction time is 120 min.
Further, the preparation method of the wheat seedling extract comprises the following steps:
firstly, cutting wheat seedlings in a growing period, wherein the height of the overground part of the wheat seedlings is 10-20cm, and then washing the wheat seedlings by water and draining the wheat seedlings;
then, cutting the wheat seedlings and mechanically extruding, and removing juice to obtain wheat seedling solid matters;
and finally, distilling the extruded solid matter, and collecting distillate, namely the wheat seedling essential oil.
Further, the preparation method of the yam peptide extract comprises the following steps:
firstly, crushing and pulping fresh Chinese yam, adding 2 times of water by volume, heating to boil, keeping for 10min, and cooling to obtain Chinese yam pulp; the rhizoma Dioscoreae is rhizoma Dioscoreae;
and then, adjusting the pH value of the yam pulp to 5.5-6.5, adding compound protease, carrying out enzymolysis for 5 hours at 50-60 ℃, heating to 80-90 ℃, inactivating enzyme, centrifuging, filtering with a membrane, concentrating and carrying out spray drying to obtain the yam peptide extract.
Furthermore, the compound protease is prepared by compounding papain and neutral protease according to the ratio of 1: 0.5.
Further, the preparation method of the plant extraction essence comprises the following steps:
step 1, mixing the humectant, the thickener, the yam peptide extract and deionized water, and stirring at 65-70 ℃ for 15-20min to obtain a water phase;
step 2, mixing the solubilizer, the lychee seed extract, the wheat seedling essential oil, the vitamin E, the emulsifier and the preservative, and uniformly stirring to obtain an oil phase;
and 3, dropwise adding the oil phase into the water phase at the rotating speed of 10000 revolutions per minute, and fully emulsifying to obtain the emulsion.
Further, the plant extract essence is smeared on the skin and then is gently massaged until being absorbed.
Compared with the prior art, the invention has the following beneficial effects:
the formula disclosed by the invention is scientific in matching, natural and safe, has no side effect, and the Chinese yam peptide extract, the lychee seed extract and the wheat seedling essential oil are synergistic in effect and play a role together, so that the effects of resisting bacteria and diminishing inflammation, stimulating the continuous growth of epidermal cells, repairing the cells and achieving the purposes of removing pockmarks and recovering the elasticity of skin are achieved.
Detailed Description
Example 1
A plant extraction cell repairing method comprises the following steps: applying the plant extract essence on skin; then gently massage until absorption.
The plant extraction essence comprises the following raw materials in percentage by weight:
Figure BDA0003178931670000031
deionized water: and (4) the balance.
The preparation method of the lychee seed extract comprises the following steps:
firstly, removing impurities from lychee seeds, rinsing, drying, crushing by a traditional Chinese medicine crusher, and then sieving by a 50-80-mesh sieve to obtain lychee seed powder;
then, supercritical CO is adopted for the lychee seed powder2Extracting with an extractorObtaining lychee seed extract; supercritical CO2The setting parameters of the extraction instrument are as follows: the pressure of the extraction kettle is 20MPa, the extraction temperature is 40 ℃, the pressure of the separation kettle is 5MPa, the temperature is 45 ℃, and CO is generated2The flow rate is 13L/h, and the extraction time is 120 min.
The preparation method of the wheat seedling extract comprises the following steps:
firstly, cutting wheat seedlings in a growing period, wherein the height of the overground part of the wheat seedlings is 10-20cm, and then washing the wheat seedlings by water and draining the wheat seedlings;
then, cutting the wheat seedlings and mechanically extruding, and removing juice to obtain wheat seedling solid matters;
and finally, distilling the extruded solid matter, and collecting distillate, namely the wheat seedling essential oil.
The preparation method of the Chinese yam peptide extract comprises the following steps:
firstly, crushing and pulping fresh Chinese yam, adding 2 times of water by volume, heating to boil, keeping for 10min, and cooling to obtain Chinese yam pulp;
and then, adjusting the pH value of the yam pulp to 5.5-6.5, adding compound protease, carrying out enzymolysis for 5 hours at 50-60 ℃, heating to 80-90 ℃, inactivating enzyme, centrifuging, filtering with a membrane, concentrating and carrying out spray drying to obtain the yam peptide extract.
The compound protease is prepared by compounding papain and neutral protease according to the ratio of 1: 0.5.
The preparation method of the plant extraction essence comprises the following steps:
step 1, mixing the humectant, the thickener, the yam peptide extract and deionized water, and stirring at 65-70 ℃ for 15-20min to obtain a water phase;
step 2, mixing the solubilizer, the lychee seed extract, the wheat seedling essential oil, the vitamin E, the emulsifier and the preservative, and uniformly stirring to obtain an oil phase;
and 3, dropwise adding the oil phase into the water phase at the rotating speed of 10000 revolutions per minute, and fully emulsifying to obtain the emulsion.
Example 2
A plant extraction cell repairing method comprises the following steps: applying the plant extract essence on skin; then gently massage until absorption.
The plant extraction essence comprises the following raw materials in percentage by weight:
Figure BDA0003178931670000041
the preparation method of the lychee seed extract is the same as that in the example 1;
wherein, the preparation method of the wheat seedling extract is the same as that of the example 1;
wherein, the preparation method of the yam peptide extract is the same as that of the embodiment 1;
the preparation method of the plant extraction essence is the same as that of the embodiment 1;
example 3
A plant extraction cell repairing method comprises the following steps: applying the plant extract essence on skin; then gently massage until absorption.
The plant extraction essence comprises the following raw materials in percentage by weight:
Figure BDA0003178931670000051
the preparation method of the lychee seed extract is the same as that in the example 1;
wherein, the preparation method of the wheat seedling extract is the same as that of the example 1;
wherein, the preparation method of the yam peptide extract is the same as that of the embodiment 1;
the preparation method of the plant extraction essence is the same as that of the embodiment 1;
comparative example 1:
the same as example 1, except for the preparation method of the lychee seed extract,
in this comparative example, the preparation method of the lychee seed extract comprises the following steps:
removing impurities from semen litchi, rinsing, oven drying, pulverizing with a traditional Chinese medicine pulverizer, and sieving with 50-80 mesh sieve to obtain semen litchi powder;
then, according to the mass ratio of the lychee seed powder to the liquid water of 1:8, adding water into the lychee seed powder, distilling, and collecting distillate, namely the lychee seed extract.
Comparative example 2:
the same as example 1, except for the preparation method of the lychee seed extract,
in this comparative example, the preparation method of the lychee seed extract comprises the following steps:
removing impurities from semen litchi, rinsing, oven drying, pulverizing with a traditional Chinese medicine pulverizer, and sieving with 50-80 mesh sieve to obtain semen litchi powder;
then, according to the mass ratio of the lychee seed powder to the water liquid of 1:8, adding water into the lychee seed powder, decocting for 2 times with slow fire, combining the decoction of the two times, concentrating, and performing spray drying to obtain the lychee seed extract.
Comparative example 3
The same as example 1, except that the litchi seed extract was not added to the raw material.
Comparative example 4:
the same as example 1, except that the complex enzyme used in the preparation process of the yam peptide extract is different.
In the comparative example, the complex enzyme used in the preparation process of the yam peptide extract is prepared from bromelain and neutral protease according to the ratio of 1: 0.5.
Comparative example 5
The same as example 1, except that the yam peptide extract was not added to the raw material.
Comparative example 6
The same as in example 1, except that not the essential oil of wheat seedlings but the extract of wheat seedlings was used, the plant extract essence was prepared by adding the extract of wheat seedlings in an aqueous phase, not an oil phase.
The preparation method of the wheat seedling extract comprises the following steps:
firstly, cutting wheat seedlings in a growing period, wherein the height of the overground part of the wheat seedlings is 10-20cm, and then washing the wheat seedlings by water and draining the wheat seedlings;
then, cutting the wheat seedlings into pieces, pulping, and collecting juice to obtain wheat seedling juice;
finally, heating the collected wheat seedling juice to inactivate enzyme, concentrating under reduced pressure, and freeze drying to obtain wheat seedling extract.
Comparative example 7
The procedure was as in example 1 except that no wheat seedling essential oil was added to the starting materials.
Effect example 1:
selecting 400 volunteers with pockmarks on the face, wherein the volunteers are 15-45 years old, 25 years old and 200 women in each year; randomly divided into 8 groups of 50 people each;
one group was tested and the essence and the application method described in example 1 were used to care skin;
comparing one group, and caring skin by adopting the essence and the using method recorded in the comparative example 1;
comparing two groups, and caring skin by adopting the essence and the using method recorded in the comparative example 2;
comparing the three groups, and caring skin by adopting the essence and the using method recorded in the comparative example 3;
comparing the four groups, and caring skin by adopting the essence and the using method recorded in the comparative example 4;
comparing the five groups, and caring skin by adopting the essence and the using method recorded in the comparative example 5;
comparing the six groups, and caring skin by adopting the essence and the using method recorded in the comparative example 6;
comparing seven groups, and caring skin by adopting the essence and the using method recorded in the comparative example 7;
the using method is carried out twice a day for 2 months continuously, the using effect is observed, and the result is shown in the following table.
The effect of use was observed according to the essence and the method of use described in each example and comparative example, twice daily for 2 months.
The curative effect is as follows: percent efficacy ═ 100% treatment-pre-treatment pock count/post-treatment pock count;
the effect is shown: pockmark subsides by 60-90%, pockmark is relieved;
the method has the following advantages: pockmark subsides by 30-60%, pockmark improves;
and (4) invalidation: pockmark regression was less than 30%, pockmark was not improved;
show effect% Effective% Not effective%
Compare a group 92 6 2
Test A set 16 60 24
Two groups of experiments 12 66 22
Three groups of experiments 8 56 46
Test four groups 22 64 14
Test five groups 14 58 28
Six groups of experiments 10 72 18
Test seven groups 6 60 44
As can be seen from the data in the table, the removal effect of any one of the yam peptide extract, the lychee seed extract and the wheat seedling essential oil on pockmarks is reduced sharply, so that: the yam peptide extract, the lychee seed extract and the wheat seedling essential oil are coordinated to achieve the purpose of removing pockmarks. In addition, the data in the table can also show that the types of enzymes used in the yam peptide extraction process, the litchi seed extraction method and the preparation method of the wheat seedling essential oil play a crucial role in the synergistic acne removing effect.
The embodiments described above are only preferred embodiments of the invention and are not exhaustive of the possible implementations of the invention. Any obvious modifications to the above would be obvious to those of ordinary skill in the art, but would not bring the invention so modified beyond the spirit and scope of the present invention.

Claims (8)

1. The plant extraction cell repairing method is characterized by comprising the following steps: applying the plant extract essence to the skin;
the plant extraction essence comprises the following raw materials in percentage by weight:
Figure FDA0003178931660000011
2. the method of repairing plant extractive cells as claimed in claim 1,
the humectant is selected from one or more of squalane, sodium alginate, jojoba oil, hyaluronic acid, collagen, hyaluronic acid and glycerol;
the emulsifier is one or more selected from glycerol stearate, polyglycerol-3 diisostearate, polyglycerol-6 distearate and polyglycerol-6 dioleate;
the thickening agent is selected from one or more of carbomer, xanthan gum and hydroxymethyl cellulose;
the solubilizer adopts PEG-40 hydrogenated castor oil;
the preservative is selected from one or more of methyl hydroxybenzoate, propyl hydroxybenzoate and propyl hydroxybenzoate;
the pH regulator adopts one or two of triethanolamine and arginine.
3. The method of repairing plant extractive cells as claimed in claim 1,
the preparation method of the lychee seed extract comprises the following steps:
firstly, removing impurities from lychee seeds, rinsing, drying, crushing by a traditional Chinese medicine crusher, and then sieving by a 50-80-mesh sieve to obtain lychee seed powder;
then, supercritical CO is adopted for the lychee seed powder2Extracting with an extractor to obtain semen litchi extract; supercritical CO2The setting parameters of the extraction instrument are as follows: extraction kettle pressure of 20MPa, extractTaking the mixture at the temperature of 40 ℃, the pressure of a separation kettle at the temperature of 45 ℃ and CO at the temperature of 5MPa2The flow rate is 13L/h, and the extraction time is 120 min.
4. The method of repairing plant extractive cells as claimed in claim 1,
the preparation method of the wheat seedling extract comprises the following steps:
firstly, cutting wheat seedlings in a growing period, wherein the height of the overground part of the wheat seedlings is 10-20cm, and then washing the wheat seedlings by water and draining the wheat seedlings;
then, cutting the wheat seedlings and mechanically extruding, and removing juice to obtain wheat seedling solid matters;
and finally, distilling the extruded solid matter, and collecting distillate, namely the wheat seedling essential oil.
5. The method of repairing plant extractive cells as claimed in claim 1,
the preparation method of the yam peptide extract comprises the following steps:
firstly, crushing and pulping fresh Chinese yam, adding 2 times of water by volume, heating to boil, keeping for 10min, and cooling to obtain Chinese yam pulp;
and then, adjusting the pH value of the yam pulp to 5.5-6.5, adding compound protease, carrying out enzymolysis for 5 hours at 50-60 ℃, heating to 80-90 ℃, inactivating enzyme, centrifuging, filtering with a membrane, concentrating and carrying out spray drying to obtain the yam peptide extract.
6. The method of claim 5, wherein the plant extract cell repair is performed by a method comprising,
the compound protease is prepared by compounding papain and neutral protease according to the ratio of 1: 0.5.
7. The method of repairing plant extractive cells as claimed in claim 1,
the preparation method of the plant extraction essence comprises the following steps:
step 1, mixing the humectant, the thickener, the yam peptide extract and deionized water, and stirring at 65-70 ℃ for 15-20min to obtain a water phase;
step 2, mixing the solubilizer, the lychee seed extract, the wheat seedling essential oil, the vitamin E, the emulsifier and the preservative, and uniformly stirring to obtain an oil phase;
and 3, dropwise adding the oil phase into the water phase at the rotating speed of 10000 revolutions per minute, and fully emulsifying to obtain the emulsion.
8. The method for repairing plant-derived cells according to claim 1, wherein the plant-derived essence is applied to the skin and gently massaged until absorbed.
CN202110841305.5A 2021-07-26 2021-07-26 Plant extraction cell repair method Withdrawn CN113576964A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115245474A (en) * 2022-08-29 2022-10-28 深圳伊赞泉生物科技有限公司 Preparation method of plant extraction essence moisturizing skin care product

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115245474A (en) * 2022-08-29 2022-10-28 深圳伊赞泉生物科技有限公司 Preparation method of plant extraction essence moisturizing skin care product

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