CN113557303A - 转基因表达的调节 - Google Patents

转基因表达的调节 Download PDF

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CN113557303A
CN113557303A CN202080020619.2A CN202080020619A CN113557303A CN 113557303 A CN113557303 A CN 113557303A CN 202080020619 A CN202080020619 A CN 202080020619A CN 113557303 A CN113557303 A CN 113557303A
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J·L·格林
T·J·凯利赫
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Abstract

披露了调节例如降低重组杀昆虫蛋白在转基因植物的雄性生殖细胞和/或组织中的表达的组合物和方法。特别地，提供了可用于此类方法中的新型重组DNA构建体，以及含有此类重组DNA构建体的转基因植物、细胞和种子。重组DNA构建体和含有此类构建体的转基因植物、细胞和种子提供了极大改进的方法，以最小化可能与雄性生殖组织中杀昆虫蛋白的表达相关的任何潜在风险。

Description

转基因表达的调节
序列表
提供ASCII文本格式的序列表作为纸质副本的替代，该序列表是根据37C.F.R.§1.821提交的，名称为“81814-US-L-ORG-P-1_ST25.txt”，大小为701千字节，于2019年3月28日生成并经由EFS-Web提交。这个序列表特此通过引用以其披露内容并入本说明书中。
技术领域
本发明披露了用于控制转基因表达的分子构建体和方法，例如，植物中的转基因抑制或抑制特定细胞或组织中靶RNA的表达。还披露了转基因真核生物(包括转基因植物细胞、植物和种子)，其基因组包括用于控制外源基因表达的分子构建体。
背景技术
转基因作物由越来越复杂的遗传修饰(包括赋予不同性状的多个转基因，也称为“基因堆叠”或“性状堆叠”)组成。例如，目前市场上的许多转基因玉米产品在同一植物内含有编码用于控制广谱昆虫有害生物的杀昆虫蛋白的多种基因、编码赋予植物对广谱化学除草剂的耐受性的蛋白的多种基因以及编码在植物转化过程中用作可选择标记物的蛋白的多种基因。许多用于控制昆虫有害生物的转基因蛋白(例如来自苏云金芽孢杆菌(Bacillusthuringiensis)的晶体内毒素(称为Cry蛋白))对鳞翅目或鞘翅目昆虫有害生物有活性。鳞翅目活性Cry蛋白的实例包括Cry1A、Cry1B、Cry1C、Cry1D、Cry1E、Cry1F和Cry9。鞘翅目活性Cry蛋白的实例包括Cry3A、Cry3B、Cry3C、Cry8、二元Cry23-Cry37以及二元Cry34-Cry35。在给定的昆虫目内，大多数个体Cry蛋白对窄谱昆虫物种有生物活性。即使具有这种窄谱活性，某些Cry蛋白可能对与目标有害生物昆虫相同的昆虫目中的某些非有害生物物种具有低到中等的活性。例如，Hellmich等人(2001)Proc.Natl.Aca.Sci.[美国科学院院刊]98:11925-11930发现对鳞翅目有害生物(例如欧洲玉米螟(European corn borer，Ostrinianubilalis))有活性的某些纯化的Cry蛋白对非有害生物鳞翅目昆虫(普累克西普斑蝶(monarch butterfly)(黑脉金斑蝶(Danaus plexippus))的一龄虫也有一定的活性。然而，靠后龄期的黑脉金斑蝶幼虫远不那么容易受影响。
目前，大多数编码杀昆虫蛋白的转基因在商业转基因作物中的表达由组成型启动子(即，在整个植物中在所有或大部分组织类型(包括花粉)中、在植物的整个生长周期中起作用的启动子)驱动。由于植物花粉可能是一些非有害生物昆虫物种的食物来源，或者假设植物花粉可能被风携带并沉积到非有害生物昆虫宿主植物上，因此调控转基因作物商业化的监管机构担心花粉中某些杀昆虫蛋白(例如，某些Cry蛋白)的高水平表达可能对非有害生物昆虫的本地化种群产生不利影响。此外，已经观察到花粉中某些杀昆虫蛋白的表达对转基因植物的雄性能育性有不利影响。例如，在玉米花粉中表达的高水平的Vip3杀昆虫蛋白可能导致某些近交遗传背景中的雄性能育性下降或完全不育(美国专利号10,214,784；通过引用并入本文)。因此，调节杀昆虫蛋白在转基因植物中的表达将是有益的，例如，使其在营养组织(大多数有害生物昆虫最初取食的地方)(例如叶组织)中高水平表达，但在花粉(一些非有害生物昆虫可能取食的植物组织)中减少表达。
大多数以时间和/或空间方式靶向植物转基因表达的研究都集中在启动子的使用上，从病毒和植物组成型启动子到组织和细胞特异性启动子，再到诱导型和合成型启动子。启动子选择对于转基因在植物中的成功基因转移和表达已经变得越来越重要。然而，通常情况下，启动子在其自然环境(例如，野生型非转基因植物)中的空间和时间功能并不能预测其用于核酸构建体中以驱动异源转基因表达时的空间和时间功能。例如，在野生型(非转基因)玉米植物中具有根特异性功能的启动子，当它被用来驱动转基因玉米中的异源转基因时，可作为组成型启动子或仅根偏好性启动子(即，在根中表达更高，但在其他组织(如营养组织)中也有渗漏表达)发挥作用。因此，需要使用启动子的替代方法或除启动子之外的替代物来控制植物中的转基因表达，以调节(例如抑制或增加)在转基因作物的某些组织、特别是在雄性生殖组织像花粉和/或绒毡层中的表达。理想地，替代方法和组合物将调节(例如抑制)编码杀昆虫蛋白的转基因在雄性生殖细胞或组织中的表达，无论使用何种启动子来驱动转基因在转基因植物中的表达。
抑制特定细胞基因、病毒或可移动遗传元件(如转座子和逆转录元件)表达的机制对多种真核生物的正常细胞功能至关重要。在植物(Matzke等人,Curr.Opin.Genet.Dev.[遗传学与发育新见]11:221-227,2001)、动物(Fire等人,Nature[自然],391:806-811,1998)和真菌(Cogoni,Annu.Rev.Microbiol.[微生物学年鉴]55:381-406,2001)中各自发现的许多相关过程导致RNA定向抑制基因表达(也称为RNA沉默)。这些过程中的每一个都由含有双链RNA(dsRNA)结构的分子(例如在RNA病毒复制过程中形成的含有反向重复或双链RNA中间体的转录物)触发。非dsRNA，也被称为异常RNA，也可作为RNA沉默的引发剂。这种异常RNA可通过沉默相关的RNA依赖性RNA聚合酶(RDR)转化为dsRNA，该RDR已在植物、真菌和线虫秀丽隐杆线虫(C.elegans)中鉴别出(Tuschl,Chem Biochem[化学生物化学],2:239-245,2001)。
已经表征了两种主要类型的小RNA：短干扰RNA(siRNA)和微小RNA(miRNA)。最终形成miRNA的初级转录物由不编码蛋白质的miRNA基因转录而成。这些转录物形成发夹结构，然后通过Dicer(或通过植物中的Dicer样活性物)加工以产生在每个3'末端含有2个碱基的突出端的小RNA双链体。通过解离双链体中的两条链形成长度大约为20-22个核苷酸的成熟的单链miRNA，并选择性地将其掺入RNA诱导沉默复合体或RISC中(Zamore,Science[科学],296:1265-1269,2002；Tang等人,Genes Dev.[基因与发育],17:49-63,2003；Xie等人,Curr.Biol.[当代生物学]13:784-789,2003)。
siRNA在化学结构上与miRNA相似，但siRNA通过由Dicer酶或DCL酶切割相对较长的双链RNA分子而产生(Zamore,Science[科学],296:1265-1269,2002；Bernstein等人,Nature[自然],409:363-366,2001)。在动物和植物中，siRNA被组装成RISC，并引导RISC的序列特异性核糖核酸溶解活性，从而导致细胞质中的mRNA、病毒RNA或其他RNA靶分子的切割。在细胞核中，siRNA还引导异染色质相关的组蛋白和DNA甲基化，导致单个基因或大染色质结构域的转录沉默。
植物和动物中的微小RNA作为参与广泛细胞过程的基因的转录后调节物而起作用(Bartel,Cell[细胞]116:281-297,2004；He和Hannon,Nat Rev Genet.[遗传学自然评论]5:522-531,2004)。在植物拟南芥(Arabidopsis thaliana)中，miRNA调节如下mRNA，这些mRNA编码至少十二个转录因子家族、几种miRNA代谢因子、以及参与应激反应、代谢和激素信号传导的蛋白质(Jones-Rhoades和Bartel,Mol Cell[分子细胞]14:787-799,2004；Kasschau等人,Dev Cell[发育细胞]4:205-217,2003；Llave等人,Science[科学]297:2053-2056,2002b；Vazquez等人,Curr Biol[当代生物学]14:346-351,2004a；Xie等人,Curr Biol[当代生物学]13:784-789,2003)。植物miRNA靶向不成比例地大量的在发育过程(包括发育时间、细胞增殖的控制、分生组织细胞功能和模式)中起作用的基因。miRNA生物发生或功能的全面破坏或miRNA-靶点相互作用的特定破坏可导致严重的发育异常(Achard等人,Development[发育]131:3357-3365,2004；Chen,Science[科学]303:2022-2025,2004；Emery等人,Curr Biol[当代生物学]13:1768-1774,2003；Juarez等人,Nature[自然]428:84-88,2004；Kidner和Martienssen,Nature[自然]428:81-84,2004；Laufs等人,Development[发育]131:4311-4322,2004；Mallory等人,Curr Biol[当代生物学]14:1035-1046,2004；Palatnik等人,Nature[自然]425:257-263,2003；Tang等人,Genes&Dev[基因与发育]17:49-63 2003；Vaucheret等人,Genes Dev[基因与发育]18:1187-1197,2004)，这表明基于miRNA的调节对正常生长和发育至关重要。大多数miRNA及其相应的靶标在相当长的进化时间内的保守性强化了这一想法(Bartel,Cell[细胞]116:281-297,2004)。已经通过直接克隆方法和计算预测策略鉴定出了微小RNA(Jones-Rhoades和Bartel,Mol Cell[分子细胞]14:787-799,2004；Llave等人,Plant Cell[植物细胞]14:1605-1619,2000a；Park等人,Curr Biol[当代生物学]12:1484-1495,2002；Reinhart等人,Genes Dev[基因与发育]16:1616-1626,2002；Sunkar和Zhu,Plant Cell[植物细胞]16:2001-2019,2004)。
植物miRNA通常与靶位点有近乎完美的互补性，这些靶位点最常见于基因组的蛋白质编码区。因此，大多数(但不是全部)植物miRNA通过与和RNAi相关的siRNA引导机制类似的机制起着引导靶标的切割的作用(Jones-Rhoades和Bartel,Mol Cell[分子细胞]14:787-799,2004；Kasschau等人,Dev Cell[发育细胞]4:205-217,2003；Llave等人,Science[科学]297:2053-2056,2002；Tang等人,Genes&Dev[基因与发育]17:49-63 2003)。相反，动物miRNA与其靶位点的互补性水平相对较低，这些靶位点最常见于靶转录物的3'非翻译区内的多个拷贝中(Lewis等人,Cell[细胞]115:787-798,2003；Rajewsky和Socci,Dev Biol[发育生物学]267:529-535,2004；Stark等人,PLoS Biol[公共科学图书馆·生物学]1:E60,2003)。大多数动物miRNA不引导切割，而是起着在翻译或共翻译水平上抑制表达的作用(Ambros,Cell[细胞]113:673-676,2003；He和Hannon,Nat Rev Genet.[遗传学自然评论]5:522-531,2004)。至少一些植物miRNA也可以作为翻译阻遏物发挥作用(Aukerman和Sakai,Plant Cell[植物细胞]15:2730-2741,2003；Chen,Science[科学]303:2022-2025,2004)。翻译阻抑不是动物miRNA的固有活性，因为如果与具有高水平互补性的靶标一起出现，则miRNA将引导切割(Doench等人,Genes Dev[基因与发育]17:438-442,2003；Hutvagner和Zamore,Science[科学]297:2056-2060,2002；Yekta等人,Science[科学]304:594-596,2004；Zeng等人,Proc Natl Acad Sci USA[美国国家科学院院刊]100:9779-9784,2003)。
通过遗传限定的RNA前体的溶核成熟来形成微小RNA，这些RNA前体采用不完整的、自身互补的折回结构。加工产生最终将miRNA链提供给效应子复合体(称为RISC)的双链体中间体(miRNA/miRNA*)(Khvorova等人,Cell[细胞]115:209-216,2003；Schwarz等人,Cell[细胞]115:199-208,2003)。植物含有四种DICER样(DCL)蛋白，其中一种(DCL1)是大多数或所有miRNA前体的成熟所必需的(Kurihara和Watanabe,Proc Natl Acad Sci USA[美国国家科学院院刊]101:12753-12758,2004；Park等人,Curr Biol[当代生物学]12:1484-1495,2002；Reinhart等人,Genes Dev[基因与发育]16:1616-1626,2002；Schauer等人,TrendsPlant Sci[植物科学趋势]7:487-491,2002)。DCL1蛋白含有RNA螺旋酶和两个RNA酶III样结构域、中央PAZ结构域和C-末端dsRNA结合基序。动物miRNA前体加工需要Drosha(另一种RNA酶III结构域蛋白)和Dicer进行连续核溶解步骤(Lee等人,Nature[自然]425:415-419,2003)。HEN1通过3'甲基化酶活性参与植物中miRNA生物发生或稳定性(Boutet等人,CurrBiol[当代生物学]13:843-848,2003；Park等人,Curr Biol[当代生物学]12:1484-1495,2002)。dsRNA结合的HYL1蛋白对于与细胞核中的DCL1和HEN1合作的miRNA生物发生是必需的。基于序列相似性，已经表明HYL1像动物R2D2一样发挥作用，R2D2在RISC组装过程中需要进行后加工(Han等人,Proc Natl Acad Sci USA[美国国家科学院院刊]101:1093-1098,2004；Liu等人,Science[科学]301:1921-1925,2003；Pham等人,Cell[细胞]117:83-94,2004；Tomari等人,Science[科学]306:1377-1380,2004；Vazquez等人,Curr Biol[当代生物学]14:346-351,2004a)。在动物中，输出蛋白-5(Exp5)通过Ran-GTP依赖性机制调节前miRNA从细胞核到细胞质的转运(Bohnsack等人,RNA 10:185-191,2004；Lund等人,Science[科学]303:95-98,2003；Yi等人,Genes Dev[基因与发育]17:3011-3016,2003)。在拟南芥属(Arabidopsis)中，HST可能提供将miRNA中间体转运至细胞质的相关功能(Bollman等人,Development[发育]130:1493-1504,2003)。植物中含有活性miRNA的RISC复合体几乎肯定含有一种或多种ARGONAUTE蛋白，例如AGO1(Fagard等人,Proc Natl Acad Sci USA[美国国家科学院院刊]97:11650-11654,2000；Vaucheret等人,Genes Dev[基因与发育]18:1187-1197,2004)。最近显示动物中的Argonaute蛋白为靶向切割提供催化活性(Liu等人,Science[科学]305:1437-1441,2004；Meister等人,Mol Cell[分子细胞]15:185-197,2004)。
除了miRNA，植物还产生内源性21-25个核苷酸的小RNA的多个组。其中大多数与miRNA的不同之处在于它们产生自双链RNA(而不是不完整的折回结构)，在某些情况下由RNA依赖性RNA聚合酶(RDR)的活性产生。拟南芥属DCL2、DCL3、DCL4、RDR1、RDR2和RDR6在siRNA生物发生中具有已知作用(Dalmay等人,Cell[细胞]101:543-553,2000；Mourrain等人,Cell[细胞]101:533-542,2000；Peragine等人,Genes&Dev[基因与发育]18:2369-2379,2004；Vazquez等人,Mol Cell[分子细胞]16:69-79,2004b；Xie等人,PLoS Biol[公共科学图书馆·生物学]2:642-652,2004；Yu等人,Mol Plant Microbe Interact[分子植物微生物相互作用]16:206-216,2003)。例如，DCL3和RDR2在异染色质相关的RNAi途径中合作，这导致由多种逆转录元件和转座子、5S rDNA基因座、内源性同向和反向重复、以及含有同向重复的转基因产生约24个核苷酸的siRNA(Xie等人,PLoS Biol[公共科学图书馆·生物学]2:642-652,2004；Zilberman等人,Science[科学]299:716-719,2003)。RDR6在正义转基因、一些病毒和由反式作用siRNA(ta-siRNA)靶向的特定内源mRNA的转录后RNAi中起作用(Dalmay等人,Cell[细胞]101:543-553,2000；Mourrain等人,Cell[细胞]101:533-542,2000；Peragine等人,Genes&Dev[基因与发育]18:2369-2379,2004；Vazquez等人,Mol Cell[分子细胞]16:69-79,2004b；Yu等人,Mol Plant Microbe Interact[分子植物微生物相互作用]16:206-216,2003)。Ta-siRNA由被RDR6(其与SGS3合作)识别的转录物产生，并且作为形成dsRNA的底物。通过DCL1以21个核苷酸的间距精确加工dsRNA，以产生一组“分阶段的”的ta-siRNA。这些ta-siRNA与靶mRNA相互作用，以通过与植物miRNA相同的机制引导切割(Peragine等人,Genes&Dev[基因与发育]18:2369-2379,2004；Vazquez等人,Mol Cell[分子细胞]16:69-79,2004)。
鉴于上述调节转基因植物中的转基因表达的问题，本发明提供了包含新型miRNA元件的新型DNA构建体，以及使用这些miRNA元件和构建体调节编码杀昆虫蛋白的转基因在转基因植物的雄性生殖组织中的表达的方法。此类组合物和方法利用在需要调节转基因表达的组织中起作用的内源性微小RNA。
发明内容
本发明总体上涉及调节例如降低重组杀昆虫蛋白在转基因植物的雄性生殖细胞和/或组织中的表达的方法，可用于此类方法中的重组DNA构建体，以及含有此类重组DNA构建体的转基因植物、细胞和种子。重组DNA构建体和含有此类构建体的转基因植物、细胞和种子提供了一种大大改进的方法，以最小化对与花粉中杀昆虫蛋白的表达相关的非有害生物昆虫物种的任何潜在风险，或用于减轻花粉和/或绒毡层中杀昆虫蛋白的表达可能对转基因植物雄性能育性产生的影响。
在一方面，本发明提供了重组DNA构建体，该重组DNA构建体包括编码重组蛋白(例如杀昆虫蛋白)的蛋白质编码序列，以及转录为能够启动与内源性植物miRNA(其是雄性组织特异性的或雄性组织优选的)的结合的微小RNA(miRNA)元件的DNA，该微小RNA元件包含例如可操作地连接至该蛋白质编码序列的miRNA起始子序列。在一个实施例中，miRNA元件包括在蛋白质编码序列的3'非翻译区内。在另一个实施例中，miRNA元件包括在蛋白质编码序列的5'非翻译区内。在另一个实施例中，miRNA元件包括在蛋白质编码序列内，例如在蛋白质编码序列的起始密码子与终止密码子之间。在另一个实施例中，miRNA元件位于蛋白质编码序列与作为3'非翻译区的一部分的聚腺苷酸化序列之间。在另一个实施例中，miRNA元件包括至少一个miRNA起始子序列。在另一个实施例中，与转基因玉米中的非雄性生殖组织(如叶组织)相比，miRNA起始子序列在转基因玉米植物中的表达降低了本发明的杀昆虫蛋白在该转基因玉米植物的雄性生殖组织中的表达。在其他实施例中，与转基因玉米中的非雄性生殖组织(如叶组织)相比，本发明的miRNA起始子序列在转基因玉米植物中的表达增加了本发明的杀昆虫蛋白在该转基因玉米植物的雄性生殖组织(如花粉)中的表达。在另一个实施例中，miRNA元件包括至少一个编码miRNA起始子序列的序列，该至少一个序列选自由SEQ ID NO:41-46或SEQ ID NO:98-101组成的组，并且该至少一个序列分别编码选自由SEQ ID NO:29-34或SEQ ID NO:94-97组成的组的miRNA起始子序列。在另一个实施例中，miRNA元件包含靶插入序列，该靶插入序列包含miRNA起始子序列和该起始子序列的5`和/或3`末端侧翼的合成核苷酸序列。在另一个实施例中，靶插入序列选自由SEQ ID NO:10-18或SEQ ID NO:79-83组成的组。在另一个实施例中，重组杀昆虫蛋白在转基因植物中的表达赋予了至少营养组织对来自昆虫有害生物的摄食损伤的耐受性。在另一个实施例中，重组杀昆虫蛋白是杀昆虫Cry蛋白或营养期杀昆虫蛋白(Vip)。在另一个实施例中，杀昆虫蛋白是Cry1A蛋白或Vip3A蛋白。在另一个实施例中，杀昆虫蛋白是Cry1Ab蛋白或Vip3Aa蛋白。在另一个实施例中，杀昆虫蛋白包含选自由SEQ ID NO:61-66或SEQ ID NO:115-117组成的组的氨基酸序列。
本发明的另一方面提供了表达盒，该表达盒包含可操作地连接至重组杀昆虫蛋白编码序列的异源启动子，该重组杀昆虫蛋白编码序列可操作地连接至编码本发明的微小RNA(miRNA)元件的DNA序列，该miRNA元件在玉米植物中是雄性组织特异性的或雄性组织优选的。在一个实施例中，表达盒包含选自由SEQ ID NO:20-28或SEQ ID NO:85-93组成的组的序列。
本发明的另一方面提供了包含本发明的DNA构建体的重组载体。在一个实施例中，重组载体包含选自由SEQ ID NO:48-59或SEQ ID NO:105-113组成的组的序列。
本发明的另一方面提供了包含本发明的DNA构建体和/或表达盒和/或重组载体的转基因玉米(玉蜀黍(maize)；玉蜀黍(Zea mays))植物。在一个实施例中，本发明提供了本发明的转基因玉米植物的转基因种子、子代或植物部分，其中该转基因种子、子代或植物部分包含本发明的DNA构建体、表达盒或重组载体。
本发明的另一方面提供了制备DNA构建体的方法，该方法包括鉴定内源性雄性组织特异性或雄性组织优选的玉米微小RNA(miRNA)；构建编码至少一个被该玉米miRNA识别的miRNA起始子序列的miRNA元件，以及将该miRNA元件可操作地连接至杀昆虫蛋白编码序列。在一个实施例中，雄性组织特异性miRNA或雄性组织优选的miRNA是花粉特异性或花粉优选的或者是绒毡层特异性或绒毡层优选的。在另一个实施例中，雄性组织特异性miRNA元件或雄性组织优选的miRNA元件是花粉特异性或花粉优选的或者是绒毡层特异性或绒毡层优选的。在另一个实施例中，miRNA元件包含选自由SEQ ID NO:10-18或SEQ ID NO:79-83组成的组的序列。在另一个实施例中，miRNA元件包括至少一个编码miRNA起始子序列的序列，该至少一个序列选自由SEQ ID NO:41-46或SEQ ID NO:98-101组成的组，并且该至少一个序列分别编码选自由SEQ ID NO:29-34或SEQ ID NO:94-97组成的组的miRNA起始子序列。
在另一方面，本发明还提供了调节和/或选择性地降低重组杀昆虫蛋白在转基因玉米植物的雄性生殖细胞或组织中的表达的方法，该方法通过在转基因玉米植物中表达重组DNA构建体来进行，该重组DNA构建体包含可操作地连接至包括本发明的miRNA元件的DNA序列的杀昆虫蛋白编码序列。在一个实施例中，miRNA元件包括至少一个miRNA起始子序列。在另一个实施例中，miRNA元件包括靶插入序列，该靶插入序列包含miRNA起始子序列和该起始子序列的5`和/或3`末端侧翼的合成核苷酸序列。在另一个实施例中，雄性生殖组织是花粉和/或绒毡层。在另一个实施例中，miRNA元件包括至少一个选自由SEQ ID NO:41-46或SEQ ID NO:98-101组成的组的miRNA起始子序列，该miRNA起始子序列分别编码选自由SEQID NO:29-34或SEQ ID NO:94-97组成的组的miRNA起始子序列。在另一个实施例中，靶插入序列选自由SEQ ID NO:10-18或SEQ ID NO:79-83组成的组。在另一个实施例中，重组杀昆虫蛋白在转基因玉米植物中的表达赋予了至少营养组织对来自昆虫有害生物的摄食损伤的耐受性。在另一个实施例中，重组杀昆虫蛋白是杀昆虫Cry蛋白或营养期杀昆虫蛋白(Vip)。在另一个实施例中，杀昆虫蛋白是Cry1A蛋白或Vip3A蛋白。在另一个实施例中，杀昆虫蛋白是Cry1Ab蛋白或Vip3Aa蛋白。在另一个实施例中，杀昆虫蛋白包含选自由SEQ IDNO:61-66或SEQ ID NO:115-117组成的组的氨基酸序列。
本发明还提供了在表达重组杀昆虫蛋白的转基因玉米植物中提高雄性能育性的方法，该重组杀昆虫蛋白导致雄性能育性降低或完全雄性不育，该方法包括向玉米植物的基因组中引入本发明的重组DNA构建体的步骤，该重组DNA构建体包含雄性不育诱导杀昆虫蛋白编码序列，该雄性不育诱导杀昆虫蛋白编码序列可操作地连接至包括miRNA元件的DNA序列，该miRNA元件调节或选择性地降低该雄性不育诱导杀昆虫蛋白在雄性生殖细胞或组织中的表达，其中包含该DNA构建体的转基因玉米植物与不包含该DNA构建体的对照植物相比具有增加的雄性能育性。在一个实施例中，雄性不育诱导蛋白是Vip3杀昆虫蛋白。在另一个实施例中，Vip3蛋白是Vip3Aa蛋白。在另一个实施例中，Vip3蛋白包含选自由SEQ ID NO:114-121组成的组的氨基酸序列。
本发明还提供了用于产生与具有降低的雄性能育性或是雄性不育的表达Vip3的玉蜀黍植物相比具有增加的雄性能育性的表达Vip3的玉米植物的方法，该方法包括：(a)提供包含本发明的重组DNA构建体的表达Vip3的第一玉米植物，该重组DNA构建体包含Vip3编码序列，该Vip3编码序列可操作地连接至包括miRNA元件的DNA序列，该miRNA元件调节或选择性地降低Vip3蛋白在雄性生殖细胞或组织中的表达；b)使(a)的重组DNA构建体渗入第二玉米植物中；以及c)选择包含重组DNA构建体的表达Vip3的玉米植物，其中miRNA元件降低了Vip3蛋白在雄性生殖组织中的水平，从而产生与没有重组DNA构建体的表达Vip3的玉蜀黍植物相比具有增加的雄性能育性的表达Vip3的玉米植物。
本发明还提供了减少来自表达至少一种杀昆虫蛋白的转基因玉米植物的花粉对易受该杀昆虫蛋白影响的非靶标昆虫物种的影响的方法，该方法包括向玉米植物的基因组中插入本发明的重组DNA构建体的步骤，该重组DNA构建体包含杀昆虫蛋白编码序列，该杀昆虫蛋白编码序列可操作地连接至包括miRNA元件的DNA序列，该miRNA元件调节或选择性地降低该杀昆虫蛋白在花粉中的表达，其中来自该转基因玉米植物的花粉与该转基因玉米植物的营养组织或与来自不包含该DNA构建体的对照植物的花粉相比具有降低的该杀昆虫蛋白的水平。
在另一方面，本发明提供提供了重组杀昆虫蛋白，该重组杀昆虫蛋白对鳞翅目昆虫有活性，其中该杀昆虫蛋白包含由雄性组织特异性或雄性组织优选的miRNA元件编码的氨基酸序列，并且其中该miRNA元件编码至少一个miRNA起始子序列。在一个实施例中，杀昆虫蛋白是Cry1蛋白或Vip3蛋白。在另一个实施例中，Cry1蛋白是Cry1A蛋白，或Vip3蛋白是Vip3A蛋白。在另一个实施例中，Cry1A蛋白是Cry1Ab蛋白，并且Vip3A蛋白是Vip3Aa蛋白。在另一个实施例中，氨基酸序列选自由SEQ ID NO:61-66或SEQ ID NO:115-117组成的组。在另一个实施例中，重组杀昆虫蛋白对鳞翅目有害生物有活性，该鳞翅目有害生物是欧洲玉米螟或玉米穗蛾。在另一个实施例中，雄性组织是花粉或绒毡层。在另一个实施例中，miRNA元件包含选自由SEQ ID NO:10-18或SEQ ID NO:74-78组成的组的序列。在另一个实施例中，miRNA起始子序列选自由SEQ ID NO:29-34或SEQ ID NO:94-97组成的组。
在另一方面，本发明提供了编码本发明的重组杀昆虫蛋白的合成多核苷酸。在一个实施例中，合成多核苷酸包含选自由SEQ ID NO:68-73或SEQ ID NO:118-121组成的组的序列。
通过学习本发明的以下说明书和非限制性实例，本发明的其他方面和优点对于本领域的技术人员而言将变得清楚。
附图说明
图1描绘了如实例6中所述的T0植物的ELISA结果。每个柱表示Cry1Ab杀昆虫蛋白在花粉(实心柱)和叶(空心柱)中的表达水平，以ng Cry1Ab/mg TSP(总可溶性蛋白)表示。误差柱是±标准误差(SE)。
序列表中的序列简述
SEQ ID NO:1是24373miR159h-3p靶插入元件的RNA序列。
SEQ ID NO:2是24374miR156i-3p靶插入元件的RNA序列。
SEQ ID NO:3是24375miR171i-5p靶插入元件的RNA序列。
SEQ ID NO:4是24376miR396b-3p靶插入元件的RNA序列。
SEQ ID NO:5是24377miR396b-3p靶插入元件的RNA序列。
SEQ ID NO:6是24379miR159h-3p靶插入元件的RNA序列。
SEQ ID NO:7是24380miR156i-3p靶插入元件的RNA序列。
SEQ ID NO:8是24381miR171i-5p靶插入元件的RNA序列。
SEQ ID NO:9是24383miR396b-3p靶插入元件的RNA序列。
SEQ ID NO:10是24373miR159h-3p靶插入序列的DNA序列。
SEQ ID NO:11是24374miR156i-3p靶插入序列的DNA序列。
SEQ ID NO:12是24375miR171i-5p靶插入序列的DNA序列。
SEQ ID NO:13是24376miR396b-3p靶插入序列的DNA序列。
SEQ ID NO:14是24377miR396b-3p靶插入序列的DNA序列。
SEQ ID NO:15是24379miR159h-3p靶插入序列的DNA序列。
SEQ ID NO:16是24380miR156i-3p靶插入序列的DNA序列。
SEQ ID NO:17是24381miR171i-5p靶插入序列的DNA序列。
SEQ ID NO:18是24383miR396b-3p靶插入序列的DNA序列。
SEQ ID NO:19是24366cry1Ab表达盒。
SEQ ID NO:20是24373cry1Ab表达盒。
SEQ ID NO:21是24374cry1Ab表达盒。
SEQ ID NO:22是24375cry1Ab表达盒。
SEQ ID NO:23是24376cry1Ab表达盒。
SEQ ID NO:24是24377cry1Ab表达盒。
SEQ ID NO:25是24379cry1Ab表达盒。
SEQ ID NO:26是24380cry1Ab表达盒。
SEQ ID NO:27是24381cry1Ab表达盒。
SEQ ID NO:28是24383cry1Ab表达盒。
SEQ ID NO:29是基序miR159h3p起始子RNA序列。
SEQ ID NO:30是t基因(tgene)miR159h-3p起始子RNA序列。
SEQ ID NO:31是基序miR156i-3p起始子RNA序列。
SEQ ID NO:32是基序miR171i-5p起始子RNA序列。
SEQ ID NO:33是基序miR396b-3p起始子RNA序列。
SEQ ID NO:34是t基因miR396b-3p起始子RNA序列。
SEQ ID NO:35是突变型miR159h-3p RNA序列。
SEQ ID NO:36是miR159h-3p RNA序列。
SEQ ID NO:37是miR156i-3p RNA序列。
SEQ ID NO:38是miR171i-5p RNA序列。
SEQ ID NO:39是miR396b-3p RNA序列。
SEQ ID NO:40是突变型miR396b-3p RNA序列。
SEQ ID NO:41是基序miR159h-3p起始子DNA序列。
SEQ ID NO:42是t基因miR159h-3p起始子DNA序列。
SEQ ID NO:43是基序miR156i-3p起始子DNA序列。
SEQ ID NO:44是基序miR171i-5p起始子DNA序列。
SEQ ID NO:45是基序miR396b-3p起始子DNA序列。
SEQ ID NO:46是t基因miR396b-3p起始子DNA序列。
SEQ ID NO:47是载体24366核苷酸序列。
SEQ ID NO:48是载体24373核苷酸序列。
SEQ ID NO:49是载体24374核苷酸序列。
SEQ ID NO:50是载体24375核苷酸序列。
SEQ ID NO:51是载体24376核苷酸序列。
SEQ ID NO:52是载体24377核苷酸序列。
SEQ ID NO:53是载体24379核苷酸序列。
SEQ ID NO:54是载体24380核苷酸序列。
SEQ ID NO:55是载体24381核苷酸序列。
SEQ ID NO:56是载体24383核苷酸序列。
SEQ ID NO:57是载体24372核苷酸序列。
SEQ ID NO:58是载体24378核苷酸序列。
SEQ ID NO:59是载体24382核苷酸序列。
SEQ ID NO:60是mCry1Ab-17氨基酸序列。
SEQ ID NO:61是24373mCry1Ab-17氨基酸序列。
SEQ ID NO:62是24374mCry1Ab-17氨基酸序列。
SEQ ID NO:63是24375mCry1Ab-17氨基酸序列。
SEQ ID NO:64是24376mCry1Ab-17氨基酸序列。
SEQ ID NO:65是24377mCry1Ab-17氨基酸序列。
SEQ ID NO:66是24379mCry1Ab-17氨基酸序列。
SEQ ID NO:67是mCry1Ab-17核苷酸序列。
SEQ ID NO:68是24373mCry1Ab-17核苷酸序列。
SEQ ID NO:69是24374mCry1Ab-17核苷酸序列。
SEQ ID NO:70是24375mCry1Ab-17核苷酸序列。
SEQ ID NO:71是24376mCry1Ab-17核苷酸序列。
SEQ ID NO:72是24377mCry1Ab-17核苷酸序列。
SEQ ID NO:73是24379mCry1Ab-17核苷酸序列。
SEQ ID NO:74是23708miR2275a-3p靶插入序列的RNA序列。
SEQ ID NO:75是23711miR2275b-5p靶插入序列的RNA序列。
SEQ ID NO:76是载体23712、23713和23714中miR2275b-3p靶插入序列的RNA序列。
SEQ ID NO:77是23715miR2275b-3p靶插入序列的RNA序列。
SEQ ID NO:78是23716miR2275b-3p靶插入序列的RNA序列。
SEQ ID NO:79是23708miR2275a-3p靶插入序列的DNA序列。
SEQ ID NO:80是23711miR2275b-5p靶插入序列的DNA序列。
SEQ ID NO:81是载体23712、23713和23714中miR2275b-3p靶插入序列的DNA序列。
SEQ ID NO:82是23715miR2275b-3p靶插入序列的DNA序列。
SEQ ID NO:83是23716miR2275b-3p靶插入序列的DNA序列。
SEQ ID NO:84是23705vip3表达盒核苷酸序列。
SEQ ID NO:85是23708vip3表达盒核苷酸序列。
SEQ ID NO:86是23711vip3表达盒核苷酸序列。
SEQ ID NO:87是23712vip3表达盒核苷酸序列。
SEQ ID NO:88是23713vip3表达盒核苷酸序列。
SEQ ID NO:89是23714vip3表达盒核苷酸序列。
SEQ ID NO:90是23715vip3表达盒核苷酸序列。
SEQ ID NO:91是23716vip3表达盒核苷酸序列。
SEQ ID NO:92是23717vip3表达盒核苷酸序列。
SEQ ID NO:93是23718vip3表达盒核苷酸序列。
SEQ ID NO:94是miR2275a-3p起始子RNA序列。
SEQ ID NO:95是miR2275b-5p起始子RNA序列。
SEQ ID NO:96是miR2275b-3p起始子RNA序列。
SEQ ID NO:97是miR2275b-3p靶起始子RNA序列。
SEQ ID NO:98是miR2275a-3p起始子DNA序列。
SEQ ID NO:99是miR2275b-5p起始子DNA序列。
SEQ ID NO:100是miR2275b-3p起始子DNA序列。
SEQ ID NO:101是miR2275b-3p靶起始子DNA序列。
SEQ ID NO:102是miRNA2275b-3p RNA序列。
SEQ ID NO:103是miR2275b-5p RNA序列。
SEQ ID NO:104是载体23705核苷酸序列。
SEQ ID NO:105是载体23708核苷酸序列。
SEQ ID NO:106是载体23711核苷酸序列。
SEQ ID NO:107是载体23712核苷酸序列。
SEQ ID NO:108是载体23713核苷酸序列。
SEQ ID NO:109是载体23714核苷酸序列。
SEQ ID NO:110是载体23715核苷酸序列。
SEQ ID NO:111是载体23716核苷酸序列。
SEQ ID NO:112是载体23717核苷酸序列。
SEQ ID NO:113是载体23718核苷酸序列。
SEQ ID NO:114是23705 Vip3氨基酸序列。
SEQ ID NO:115是23716 Vip3氨基酸序列。
SEQ ID NO:116是23717 Vip3氨基酸序列。
SEQ ID NO:117是23718 Vip3氨基酸序列。
SEQ ID NO:118是23705 Vip3核苷酸序列。
SEQ ID NO:119是23716 Vip3核苷酸序列。
SEQ ID NO:120是23717 Vip3核苷酸序列。
SEQ ID NO:121是23718 Vip3核苷酸序列。
SEQ ID NO:122是t基因输入序列。
SEQ ID NO:123是t基因输出序列。
SEQ ID NO:124是基序输入序列。
SEQ ID NO:125是基序输出序列。
具体实施方式
本说明不旨在是可以实施本发明的所有不同方式，或可以添加到本发明中的所有特征的详细目录。例如，关于一个实施例所说明的特征可以并入其他实施例中，并且关于一个特定实施例所说明的特征可以从那个实施例删除。因此，本发明考虑了，在本发明的一些实施例中，可以排除或省略本文陈述的任何特征或特征的组合。此外，鉴于本披露内容，本文提出的不同实施例的众多变化以及附加对于本领域技术人员将是显而易见的，这不脱离本发明。因此，以下说明旨在阐述本发明的一些特定实施例，并且并没有穷尽地叙述其所有排列、组合和变化。
除非另外定义，本文所用的所有技术和科学术语均具有与本发明所属领域的普通技术人员通常所理解的相同的含义。在本文的发明的说明中使用的术语是仅出于描述特定实施例的目的，且并不旨在限制本发明。分子生物学中常用术语的定义可在以下文献中找到：Benjamin Lewin,Genes V[基因V],由Oxford University Press[牛津大学出版社]出版,1994(ISBN 0-19-854287-9)；Kendrew等人(编辑),The Encyclopedia of MolecularBiology[分子生物学百科全书],由Blackwell Science Ltd.[布莱克威尔科学出版公司]出版,1994(ISBN 0-632-02182-9)；以及Robert A.Meyers(编辑),Molecular Biology andBiotechnology:a Comprehensive Desk Reference[分子生物学与生物技术：综合案头参考],由VCH Publishers,Inc.[VCH出版公司]出版,1995(ISBN 1-56081-569-8)。
本文引用的所有的公开、专利申请、专利以及其他参考文件对于引用中提及的有关句子和/或段落的传授内容通过引用以其全文并入。
本文提供的核苷酸序列以5'至3'方向从左至右表示，并且使用代表核苷酸碱基的标准代码表示，如37CFR§§1.821-1.825和世界知识产权组织(WIPO)标准ST.25中所述，例如：腺嘌呤(A)、胞嘧啶(C)、胸腺嘧啶(T)、以及鸟嘌呤(G)。
氨基酸同样是使用WIPO标准ST.25来指示，例如：丙氨酸(Ala；A)、精氨酸(Arg；R)、天冬酰胺(Asn；N)、天冬氨酸(Asp；D)、半胱氨酸(Cys；C)、谷氨酰胺(Gln；Q)、谷氨酸(Glu；E)、甘氨酸(Gly；G)、组氨酸(His；H)、异亮氨酸(Ile；1)、亮氨酸(Leu；L)、赖氨酸(Lys；K)、甲硫氨酸(Met；M)、苯丙氨酸(Phe；F)、脯氨酸(Pro；P)、丝氨酸(Ser；S)、苏氨酸(Thr；T)、色氨酸(Trp；W)、酪氨酸(Tyr；Y)、以及缬氨酸(Val；V)。
除非上下文另外指示，明确地预期的是本文所述的本发明的不同特征可以按任何组合使用。而且，本发明还考虑到在本发明的一些实施例中，本文陈述的任何特征或特征的组合可以被排除或省略。举例说明，如果本说明书陈述组合物包含组分A、B和C，明确地预期A、B或C的任何一种或其组合可单一地或以任何组合被省略和放弃。
为了清晰起见，定义了在本说明书中所使用的某些术语并且将其呈现如下：
如本文和所附权利要求所使用的，单数形式“一个/一种(a/an)”和“所述/该(the)”包括复数指代物，除非上下文另外明确地指示。因此，例如，提及“一种植物”是提及一种或多种植物并且包括本领域技术人员已知的其等效物等。
如本文所使用的，术语“和/或”是指并且涵盖一个或多个相关联的列出项的任何及全部可能组合，连同当以可替代性(“或”)解释时组合的缺少。
术语“约”本文用于意指大约、大致、约或在……左右。当术语“约”结合数值范围来使用时，它通过将边界延伸至高于以及低于所阐述的数值来限定这个范围。一般而言，术语“约”本文用于将数值限定至以20％的变化，优选地10％上下(更高或更低)，高于以及低于规定值。关于温度，术语“约”意指±1℃，优选±0.5℃。当术语“约”被用于本发明的上下文中(例如与温度或分子量值组合)时，确切值(即，无“约”)是优选的。
如本文所使用的，术语“扩增的”意指使用至少一种核酸分子作为模板，构建核酸分子的多个拷贝或与该核酸分子互补的多个拷贝。扩增系统包括聚合酶链式反应(PCR)系统、连接酶链式反应(LCR)系统、基于核酸序列的扩增(NASBA，安大略省密西索加的坎基尼公司(Cangene，Mississauga，Ontario))、Q-β复制酶系统、基于转录的扩增系统(TAS)、以及链置换扩增(SDA)。参见，例如，Diagnostic Molecular Microbiology:Principles andApplications[诊断分子微生物学：原理与应用],PERSING等人编,American Society forMicrobiology[美国微生物学会],华盛顿(Washington,D.C.),(1993)。扩增的产物被称为“扩增子”。
本发明的杀昆虫蛋白的“活性”意指杀昆虫蛋白作为口服活性的昆虫控制剂发挥作用，具有毒性作用、和/或能够干扰或阻止昆虫摄食，这可能引起或者可能不引起昆虫的死亡。当本发明的杀昆虫蛋白被递送至昆虫时，这种结果典型地是该昆虫的死亡，或者该昆虫不以该杀昆虫蛋白可被该昆虫可用的来源为食。“杀有害生物”被定义为有毒的生物活性，其能够控制有害生物(如昆虫、线虫、真菌、细菌或病毒)，优选地通过杀死或破坏它们来进行控制。“杀昆虫”被定义为有毒的生物活性，其能够控制昆虫，优选地通过杀死它们来进行控制。“杀有害生物剂”是具有杀有害生物活性的药剂。“杀昆虫剂”是具有杀昆虫活性的药剂。
如本文所使用的，术语“回交(backcross)”和“使回交(backcrossing)”是指如下方法，凭借该方法将子代植物与其亲本之一回交一代或多代(例如，2代或更多代、3代或更多代、4代或更多代、5代或更多代、6代或更多代、7代或更多代等)。在回交方案中，“供体”亲本是指具有待渗入的所希望的基因或DNA构建体或基因座的亲本植物。“受体”亲本(使用一次或多次)或“轮回”亲本(使用两次或更多次)是指基因或DNA构建体或基因座被渗入其中的亲本植物。例如，参见Ragot等人Marker-assisted Backcrossing:A Practical Example[标记辅助回交：实践范例],在TECHNIQUES ET UTILISATIONS DES MARQUEURSMOLECULAIRES LES COLLOQUES[分子标记技术和应用专题讨论会]中,第72卷,第45-56页(1995)；以及Openshaw等人,Marker-assisted Selection in Backcross Breeding[回交育种中的标记辅助选择],在PROCEEDINGS OF THE SYMPOSIUM"ANALYSIS OF MOLECULARMARKER DATA"[专题讨论会会议记录“分子标记数据分析”]中,第41-43页(1994)。初始杂交产生F1代。术语“BC1”是指第二次使用轮回亲本，“BC2”是指第三次使用轮回亲本，等。在实施例中，针对所希望的基因或基因座(例如，在来自子代植物或植物部分的核酸样品中)的存在鉴定和/或选择至少一代或多代子代。在实施例中，针对所希望的基因或DNA构建体或基因座的存在，鉴定和/或选择子代的两代或更多代(或甚至所有代)。
如本文所使用的术语“嵌合构建体”或“嵌合基因”或“嵌合多核苷酸”或“嵌合核酸”或“嵌合蛋白”(或类似术语)是指如下构建体或核酸分子或蛋白质，该构建体或核酸分子或蛋白质分别包含被组装进单个核酸分子或蛋白质中的不同来源的两个或更多个多核苷酸或氨基酸基序或结构域。术语“嵌合构建体”、“嵌合基因”、“嵌合多核苷酸”或“嵌合核酸”是指如下任何构建体或分子，该构建体或分子含有但不限于(1)多核苷酸(例如，DNA)，包括在自然界中没有被发现在一起的调节多核苷酸和编码多核苷酸(即，构建体中的至少一个多核苷酸相对于它的其他多核苷酸中的至少一个是异源的)，或(2)编码不是天然毗邻的蛋白部分的多核苷酸，或(3)不是天然毗邻的启动子部分。另外，嵌合构建体、嵌合基因、嵌合多核苷酸或嵌合核酸可以包含衍生自不同来源的调节多核苷酸和编码多核苷酸，或包含衍生自相同来源、但以与在自然界中所发现的不同的方式进行布置的调节多核苷酸和编码多核苷酸。在本发明的一些实施例中，嵌合构建体、嵌合基因、嵌合多核苷酸或嵌合核酸包含表达盒，该表达盒包含在调节多核苷酸的控制下、特别地在植物或细菌中具有功能性的调节多核苷酸的控制下的本发明的多核苷酸。
“编码序列”是转录成RNA(如mRNA、rRNA、tRNA、snRNA、正义RNA或反义RNA)的核酸序列。在一些方面，RNA进而在生物(如玉米植物)中被翻译以产生蛋白质(例如，本发明的杀昆虫蛋白)。在其他方面，RNA不被翻译以产生蛋白质，而是作为RNA分子起作用以调节本发明的重组杀昆虫蛋白的表达。
如本文所使用的，术语“完全可育”是指至少与对照植物一样可育的植物(例如，其亲本中的一个或两个、缺乏本发明的vip3编码序列的近等基因植物等)。在一些实施例中，“完全可育”植物在紧接着花药挤出后的三天内每天每穗释放至少与对照植物同样多的花粉粒。在一些实施例中，“完全可育”植物在紧接着花药挤出后的三天内每天每穗释放比对照植物更多的花粉粒。
如本文所使用的，“密码子优化的”序列意指如下核苷酸序列，其中这些密码子被选择以反映宿主细胞或生物可以具有的特定的密码子偏好性。这典型地是以这样一种方式来完成，该方式是为了保持由待优化的核苷酸序列所编码的多肽的氨基酸序列。在某些实施例中，本发明的重组DNA构建体的DNA序列包括针对该构建体有待在其中进行表达的细胞(例如，动物、植物、或真菌细胞)进行了优化的密码子。例如，有待在植物细胞中表达的构建体可以使其全部或部分序列(例如，第一基因抑制元件或基因表达元件)进行密码子优化用于在植物中表达。参见例如，美国专利号6,121,014，通过引用并入本文。
术语“包含(comprises或comprising)”当用于本说明书中时指示所说明的特征、整数、步骤、操作、要素、或组分的存在，但并不排除一个或多个其他特征、整数、步骤、操作、要素、组分、或其组的存在或添加。
如本文所使用的，过渡短语“基本上由……组成”(以及语法变体)意指，权利要求书的范围有待被解读为涵盖权利要求书中所列举的指定材料或步骤以及不实质上改变所要求的发明的一个或多个基本和新颖特征的那些。因此，当用于本发明的权利要求中时，术语“基本上由……组成”并不旨在被解释为等同于“包含(comprising)”。
“控制”昆虫意指通过毒性作用抑制昆虫有害生物存活、生长、摄食、或繁殖的能力，或者限制昆虫相关的作物植物损害或损失，或者保护在昆虫有害生物存在的条件下生长时的作物的产量潜力。“控制”昆虫可以是或可以不是意指杀死昆虫，尽管其优选意指杀死昆虫。
如本文所使用的，术语“玉米”与术语“玉蜀黍(maize或Zea mays)”同义。
在本发明的上下文中，“对应于(corresponding to或corresponds to)”意指当变体或同系物Cry蛋白的氨基酸序列与彼此比对时，“对应于”在该变体或同系物蛋白中某些枚举的位置的氨基酸是与参考蛋白中的这些位置比对的那些，但在相对于本发明的特定参考氨基酸序列而言的这些精确的数字位置中是不必要的。例如，如果SEQ ID NO:114是参考序列，并且与SEQ ID NO:115比对，则SEQ ID NO:115的位置201处的氨基酸Phe(Phe201)“对应于”SEQ ID NO:114的位置183处的Phe(Phe183)，或例如SEQ ID NO:117的Glu199“对应于”SEQ ID NO:114的Lys195。
如本文所使用的，术语“杂交(cross)”或“经杂交的(crossed)”是指通过授粉融合配子以产生子代(例如，细胞、种子或植物)。该术语包括有性杂交(一个植物由另一个授粉)和自交(自花授粉，例如当花粉和胚珠是来自相同的植物时)两者。术语“使杂交(crossing)”是指通过授粉使配子融合以产生子代的行为。
术语“结构域”是指沿着进化相关蛋白的序列的比对在特定位置处保守的一组氨基酸。虽然其他位置上的氨基酸可在同系物之间有所不同，但是在特定位置处高度保守的氨基酸指示在蛋白质的结构、稳定性或功能中很可能是必需的氨基酸。通过其在蛋白质同系物家族的经比对序列中的高度保守性进行鉴别，其可用作鉴别物(identifier)，用来确定所讨论的任何多肽是否属于先前鉴别的多肽组。
“控昆虫有效量”意指杀昆虫蛋白的浓度，其通过毒性作用抑制昆虫存活、生长、摄食和/或繁殖的能力，或限制昆虫相关的损害或作物植物损失。“控昆虫有效量”可以是或可以不是意指杀死昆虫，尽管其优选意指杀死昆虫。
如本文所使用的“表达盒”意指能够在适当的宿主细胞中指导特定的核苷酸序列的表达的核酸序列，包含可操作地连接至目的核苷酸序列的启动子，该目的核苷酸序列可操作地连接至终止信号。它还典型地包含适当翻译该核苷酸序列所需要的序列。包含该目的核苷酸序列的表达盒可能具有其组分中的至少一种，该组分相对于它的其他组分中的至少一种而言是异源的。该表达盒还可以是天然存在的但已经是以对于异源表达有用的重组形式而获得的表达盒。表达盒的这种用途使它如此在其被引入的细胞中不是天然存在的。然而，典型地，表达盒相对于宿主而言是异源的，即该表达盒的特定核酸序列不是天然存在于宿主细胞中的，并且必须已经通过转化过程引入到宿主细胞或宿主细胞的祖先中。在表达盒中核苷酸序列的表达可以是在组成型启动子或诱导型启动子的控制之下，该启动子只有当该宿主细胞暴露于一些特定的外界刺激时才引发转录。在多细胞生物体(例如植物)的情况下，该启动子对于特定组织、或器官、或者发育阶段也可以是特异的。
表达盒还可以任选地包括在植物中发挥作用的转录和/或翻译终止区(即，终止区)。多种转录终止子是可供用于在表达盒中使用的并且负责在超出目的异源核苷酸序列时的转录终止以及正确的mRNA聚腺苷酸化。终止区对于转录起始区可以是天然的，对于可操作地连接的目的核苷酸序列可以是天然的，对于植物宿主可以是天然的，或者可以是衍生自另一种来源(即，对于启动子、目的核苷酸序列、植物宿主、或其任何组合而言是外来的或异源的)。适当的转录终止子包括但不限于CAMV 35S终止子、tml终止子、胭脂碱合酶终止子和/或豌豆rbcs E9终止子。这些终止子可以在单子叶植物和双子叶植物两者中使用。此外，可以使用编码序列的天然转录终止子。任何已知在植物中发挥作用的可用的终止子均可以在本发明的上下文中使用。
当参考多核苷酸(如植物的基因、开放阅读框(ORF)或其部分、或转基因)而使用时，术语“表达”是指通过基因的“转录”(即经由RNA聚合酶的酶促作用)将基因中编码的遗传信息转化为RNA(例如，mRNA、rRNA、tRNA或snRNA)，并在适用的情况下(例如，如果基因编码蛋白)通过mRNA的“翻译”转化为蛋白质的过程。基因表达可以在该过程的许多阶段进行调节。例如，在反义构建体或dsRNA构建体的情况下，各自地表达可仅指该反义RNA或仅指dsRNA的转录。在本发明的一些实施例中，“表达”是指正义(mRNA)或功能性RNA的转录和稳定累积。在本发明的一些实施例中，“表达”是指蛋白质的产生。
“基因”是位于基因组内并且包含编码核酸序列的限定区域，并且典型地还包含其他负责控制该编码部分表达(也就是转录和翻译)的主要调节性核酸。基因还可以包含其他5'和3'未翻译序列和终止序列。另外的可能存在的元件是例如内含子。如在自然界中所发现，基因的调节核酸序列在正常情况下可能不与该相关联的核酸序列进行可操作地连接，并因此不会是嵌合基因。
“目的基因”是指当转移至植物时，在该植物上赋予所希望的性状(如抗生素抗性、病毒抗性、昆虫抗性、疾病抗性、或对其他有害生物的抗性、除草剂耐受性、非生物胁迫耐受性、雄性不育、改性脂肪酸代谢、改性碳水化合物代谢、改善的营养价值、工业过程中改善的性能或改变的繁殖能力)的任何核酸分子。“目的基因”还可以是被转移至植物用于在该植物中产生商业上有价值的酶或代谢物的基因。
“异源”核酸序列或核酸分子是天然地不与将该核酸序列引入其中的宿主细胞相关联的核酸序列或核酸分子，包括天然存在的核酸序列的非天然存在的多个拷贝。异源核酸序列或核酸分子可以包含嵌合序列，如嵌合表达盒，在该表达盒中，启动子和编码区源自多源生物。启动子序列可以是组成型启动子序列、组织特异性启动子序列、化学诱导型启动子序列、伤口诱导型启动子序列、胁迫诱导型启动子序列、或发育阶段特异性启动子序列。
“同源”核酸序列是与其被引入的宿主细胞天然相关联的核酸序列。
在两个核酸或氨基酸序列的上下文中，术语“同一性”或“相同的”或“基本上相同的”是指当针对最大对应性进行比较和比对时具有至少60％、优选至少80％、更优选90％、甚至更优选95％、并且最优选至少99％核苷酸或氨基酸残基同一性的两个或更多个序列或子序列，如使用以下序列比较算法之一或通过目测检查所测量的。优选地，基本的同一性存在于整个具有长度为至少约50个残基或碱基的序列的区域中，更优选地在整个至少约100个残基或碱基的区域中，并且最优选地这些序列在至少约150个残基或碱基上是基本上相同的。在尤其优选的实施例中，在整个编码区域长度中的序列是基本上相同的。此外，基本上相同的核酸或氨基酸序列基本上执行相同的功能。
对于序列比较，典型地，一个序列充当与测试序列进行比较的参考序列。当使用序列比较算法时，将测试序列和参考序列输入到计算机中(如有必要，指定子序列坐标)，并且指定序列算法程序的参数。然后，该序列比较算法基于所指定的程序参数来计算这个或这些测试序列相对于该参考序列的序列同一性百分比。
用于比较的序列的最佳比对可以按照以下方式进行，例如通过Smith和Waterman,Adv.Appl.Math.[应用数学进展]2:482(1981)的局部同源性算法、通过Needleman和Wunsch,J.Mol.Biol.[分子生物学杂志]48:443(1970)的同源比对算法、通过Pearson和Lipman,Proc.Nat'l.Acad Sci.USA[美国国家科学院院刊]85:2444(1988)的相似性方法的搜索，通过这些算法的计算机化实施(威斯康星州遗传学分析软件包中的GAP、BESTFIT、FASTA和TFASTA，遗传学计算机组(Genetics Computer Group),科学街575号(575ScienceDr.),麦迪逊,威斯康星州)，或通过目测检查(总体上参见Ausubel等人,下文)。
适于确定序列同一性百分比以及序列相似性的算法的一个实例是BLAST算法，其描述于以下文献中：Altschul等人,J.Mol.Biol.[分子生物学杂志]215:403-410(1990)。执行BLAST分析的软件是通过国家生物技术信息中心(the National Center forBiotechnology Information，美国国家医学图书馆(U.S.National Library ofMedicine)，美国洛克维尔大道8600号(8600Rockville Pike)，贝塞斯达，马里兰州20894)可供公众使用的。这种算法涉及首先通过鉴定查询序列中具有长度W的短字码而鉴定得分高的序列对(HSP)，这些得分高的序列对当与数据库序列中具有相同长度的字码(word)进行比对时匹配或满足一些正值阈值的得分T。T被称为邻近字码得分阈(Altschul等人,1990)。这些初始的邻近字码命中充当种子用于起始搜索以发现含有它们的较长的HSP。然后，将这些字码命中在两个方向上沿着每个序列延伸直到累积的比对得分可以增加。对于核苷酸序列，使用参数M(对于一对匹配残基的奖赏得分；总是>0)和N(对于错配残基的罚分；总是<0)来计算累积的得分。对于氨基酸序列，使用得分矩阵来计算累积得分。当累积的比对得分从它的最大达到值降低了数量X；由于累积一个或多个负得分的残基比对使累积得分趋于0或0以下；或者到达任一序列的末端时，停止这些字码命中在每个方向上的延伸。BLAST算法的参数W、T、以及X决定了比对的灵敏度与速度。BLASTN程序(对核苷酸序列来说)使用字长(W)为11、期望值(E)为10、截止值(cutoff)为100、M＝5、N＝-4、以及两条链的比较作为默认值。对于氨基酸序列，BLASTP程序使用字长(W)为3、期望值(E)为10、以及BLOSUM62评分矩阵作为默认值(参见Henikoff和Henikoff,Proc.Natl.Acad Sci.USA[美国国家科学院院刊]89:10915(1989))。
除了计算序列同一性百分比之外，BLAST算法还进行两个序列之间相似性的统计分析(参见例如，Karlin和Altschul,Proc.Nat'l.Acad.Sci.USA[美国国家科学院院刊]90:5873-5787(1993))。由BLAST算法提供的相似性的一种量度是最小概率总和(P(N))，它提供了在两个核苷酸或氨基酸序列之间会偶然发生匹配的概率的指示。例如，若在测试核酸序列与参考核酸序列的比较中最小概率总和小于约0.1、更优选地小于约0.01、并且最优选地小于约0.001，则该测试核酸序列被认为是与该参考序列相似的。
两个核酸序列基本上相同的另一个指示是这两种分子在严格条件下彼此杂交。短语“特异性杂交”是指分子在严格条件下仅与特定的核苷酸序列结合、双链化或杂交，这是在该序列存在于复合混合物(例如，总细胞的)DNA或RNA中时进行的。“基本上结合”是指在探针核酸与靶核酸之间的互补杂交，并且涵盖少量错配，这些错配可以通过降低杂交介质的严格来容纳，以实现靶核酸序列的所希望的检测。
在核酸杂交实验(如DNA杂交和RNA杂交)的上下文中，“严格杂交条件”和“严格杂交洗涤条件”是序列依赖性的，并且在不同的环境参数下是不同的。较长的序列在较高的温度下特异性杂交。对核酸杂交的广泛指导见于以下文献中：Tijssen(1993)LaboratoryTechniques in Biochemistry and Molecular Biology-Hybridization with NucleicAcid Probes[生物化学和分子生物学实验室技术-使用核酸探针的杂交]第2章第I部分“Overview of principles of hybridization and the strategy of nucleic acidprobe assays[杂交原理和核酸探针测定策略综述]”Elsevier[爱思唯尔集团],纽约。通常，高严格杂交和洗涤条件在限定的离子强度和pH下被选定为比特定序列的热熔点(Tm)低约5℃。典型地，在“严格条件”下，探针将会与它的靶子序列进行杂交，但不会与其他序列杂交。
Tm是50％的靶序列与完全匹配的探针进行杂交时的温度(在限定的离子强度和pH下)。非常严格条件被选定为等于特定探针的Tm。对于互补核酸(它们在DNA或RNA印迹中在滤器上具有超过100个互补的残基)的杂交的严格杂交条件的实例是在42℃下、具有1mg肝素的50％甲酰胺、将杂交进行过夜。高严格洗涤条件的实例是0.15M NaCl在72℃下持续约15分钟。严格洗涤条件的实例是0.2×SSC洗涤在65℃下持续15分钟(参见，Sambrook，下文，对于SSC缓冲液的说明)。通常，高严格洗涤之前会先进行低严格洗涤，以去除背景探针信号。对于例如超过100个核苷酸的双链体的中等严格洗涤的实例是1×SSC在45℃下持续15分钟。对于例如超过100个核苷酸的双链体的低严格洗涤的实例是4-6×SSC在40℃下持续15分钟。对于短探针(例如，约10至50个核苷酸)，严格条件典型地涉及小于约1.0M的Na离子的盐浓度，典型地在pH 7.0至8.3下约0.01至1.0M的Na离子浓度(或其他盐类)，并且该温度典型地是至少约30℃。还可以通过添加去稳定剂(如甲酰胺)来达到严格条件。一般而言，相比于不相关的探针，在特定的杂交测定中观察到高出2倍(或更高)的信噪比就表明检测到特异性杂交。如果在严格条件下彼此不杂交的核酸所编码的蛋白是基本上相同的，则它们仍然是基本上相同的。例如，当使用遗传密码允许的最大程度的密码子简并而创建核酸的拷贝时，则发生这种情况。
以下是可以用来克隆同源核苷酸序列(这些序列与本发明的参考核苷酸序列基本上相同)的杂交/洗涤条件的设置的实例：参考核苷酸序列在以下条件下优选地与该参考核苷酸序列杂交：在7％十二烷基硫酸钠(SDS)、0.5M NaPO4、1mM EDTA中在50℃，并且在2×SSC、0.1％SDS中在50℃洗涤；更令人希望的是在7％十二烷基硫酸钠(SDS)、0.5M NaPO4、1mM EDTA中在50℃，并且在1×SSC、0.1％SDS中在50℃洗涤；仍更令人希望的是在7％十二烷基硫酸钠(SDS)、0.5M NaPO4、1mM EDTA中在50℃，并且在0.5×SSC、0.1％SDS中在50℃洗涤；优选地在7％十二烷基硫酸钠(SDS)、0.5M NaPO4、1mM EDTA中在50℃，并且在0.1×SSC、0.1％SDS中在50℃洗涤；更优选地在7％十二烷基硫酸钠(SDS)、0.5M NaPO4、1mM EDTA中在50℃，并且在0.1×SSC、0.1％SDS中在65℃洗涤。
两个核酸序列或蛋白基本上相同的另一个指示是由第一核酸编码的蛋白与由第二核酸编码的蛋白进行免疫性交联反应或与其特异性结合。因此，蛋白质典型地是与第二蛋白质基本上一致的，例如其中这两种蛋白质仅区别于保守性取代。
术语“分离的”核酸分子、多核苷酸或蛋白质是不再存在于其天然环境中的核酸分子、多核苷酸或蛋白质。本发明的分离的核酸分子、多核苷酸或蛋白质可以按照纯化的形式存在，或者可以存在于重组宿主中，例如转基因细菌或转基因植物中。因此，如本文所列举的对“分离的”核酸分子的要求涵盖包含在转基因植物基因组内的核酸分子。
如本文所使用的，术语“微小RNA”(miRNA)是指长度为大约18-26个核苷酸并且在多种生物(包括动物和植物)中发现的小的非编码RNA基因产物。miRNA在结构上类似于siRNA，不同之处在于miRNA产生于源自miRNA基因的结构化的、折返形成的前体转录物。miRNA基因的初级转录物形成发夹结构，这些发夹结构被多结构域RNA酶III样核酸酶DICER和DROSHA(在动物中)或DICER-LIKE1(DCL1；在植物中)加工以产生miRNA双链体。在双链体解旋后，成熟的miRNA掺入RISC复合体中。植物miRNA通过首先结合到与miRNA完全或接近完全互补的起始子序列来与其RNA靶标相互作用。
“核酸分子”或“核酸序列”是可以从任何来源中分离的单链或双链DNA或RNA的区段。在本发明的上下文中，核酸分子典型地是DNA的区段。在一些实施例中，本发明的核酸分子是分离的核酸分子。
“可操作地连接”是指在单一核酸片段上多核苷酸的关联，这样使得一者的功能影响另一者的功能。例如，当启动子能够影响编码多核苷酸或功能RNA的表达时(即，该编码多核苷酸或功能RNA处于该启动子的转录控制之下)，则该启动子与该编码多核苷酸或功能RNA是可操作地连接的。正义方向或者反义方向的编码多核苷酸能够与调节多核苷酸可操作地连接。
如本文所使用的“杀有害生物”、“杀昆虫”等是指本发明的Cry蛋白或营养期杀昆虫蛋白(Vip)控制有害生物的能力或者可以控制如本文所定义的有害生物的Cry蛋白或Vip的量。因此，杀有害生物Cry蛋白或Vip可以杀死或抑制有害生物(例如，昆虫有害生物)存活、生长、摄食、或繁殖的能力。
术语“蛋白质”、“肽”和“多肽”在本文中可互换使用。
“植物”是在发育的任何阶段的任何植物，特别是种子植物。
“植物细胞”是植物的结构和生理单位，包含原生质体和细胞壁。植物细胞可以处于分离的单个细胞或培养细胞的形式，或者是作为较高级的组织单位(如例如，植物组织、植物器官、或全株植物)的一部分。
“植物细胞培养物”意指植物单元(例如像，原生质体、细胞培养物细胞、植物组织中的细胞、花粉、花粉管、胚珠、胚囊、接合子以及处于不同发育阶段的胚)的培养物。
“植物材料”是指叶、茎、根、花或花的部分、果实、花粉、卵细胞、接合子、种子、切条、细胞或组织培养物、或植物的任何其他部分或产物。
“植物器官”是植物的独特而明显的已结构化并且分化的部分，如根、茎、叶、花蕾或胚。
如本文所使用的“植物组织”意指组织化成结构和功能单元的一组植物细胞。包括植物中或培养物中的任何植物组织。这个术语包括但不限于全株植物、植物器官、植物种子、组织培养物以及被组织化成结构和/或功能单元的任何植物细胞群组。这个术语与如以上列出的或由该定义以其他方式涵盖的任何具体类型的植物组织的联合应用或单独应用并不旨在排除任何其他类型的植物组织。例如，“绒毡层”是玉米植物孢子囊(特别是花药)内的组织，为生长的孢子提供营养。
“多核苷酸”是指由共价键合于链中的许多核苷酸单体构成的聚合物。此类“多核苷酸”包括DNA、RNA、经修饰的寡核苷酸(例如，包含对于生物RNA或DNA不典型的碱基的寡核苷酸，如2'-O-甲基化寡核苷酸)等。在一些实施例中，核酸或多核苷酸可以是单链的、双链的、多链的或其组合。除非另有说明，否则本发明的具体核酸或多核苷酸任选地包含或编码除明确指示的任何多核苷酸之外的互补多核苷酸。
“目的多核苷酸”是指任何多核苷酸，当其转移至生物(例如，植物)中时赋予该生物所希望的特征，如昆虫抗性、疾病抗性、除草剂耐受性、抗生素抗性、改进的营养价值、工业过程中改进的性能、商业上有价值的酶或代谢物的产生、或者改变的繁殖能力。
“启动子”是编码区的不被翻译的DNA序列上游，其含有RNA聚合酶结合位点并且起始DNA的转录。启动子区还可以包括充当基因表达的调节物的其他元件。
如本文所使用的，术语“重组”是指核酸分子(例如，DNA或RNA)或蛋白或生物的如下形式，该形式通常不会在自然界中发现并且正因为如此通过人类干预来产生。如本文所使用的，“重组核酸分子”是包括多核苷酸组合的核酸分子，这些多核苷酸不会天然地一起存在并且是人类干预的结果，例如，由至少两种彼此异源的多核苷酸的组合组成的核酸分子，或人工合成的(例如，使用组装的核苷酸序列合成的多核苷酸)并且包含偏离通常存在于自然界中的多核苷酸的多核苷酸的核酸分子，或包含人工掺入至宿主细胞的基因组DNA中和该宿主细胞基因组相关侧翼DNA中的转基因的核酸分子。重组核酸分子的另一个实例是由将转基因插入至植物的基因组DNA中产生的DNA分子，其可以最终导致该生物中的重组RNA/或蛋白分子的表达。如本文所使用的，“重组植物”是通常不会在自然界中存在的植物，是人类干预的结果，并且含有掺入至其基因组中的转基因和/或异源核酸分子。由于此类基因组改变，重组植物明显不同于相关的野生型植物。
“调节元件”是指参与控制核苷酸序列的表达的序列。调节元件包含可操作地连接至目的核苷酸序列的启动子以及终止信号。它们还典型地涵盖适当翻译该核苷酸序列所需的序列。
“转化”是用于将异源核酸引入到宿主细胞或生物的方法。在特定实施例中，“转化”意指DNA分子稳定地整合到目的生物的基因组(核或质体)中。
“转化的/转基因的/重组的”是指异源核酸分子已经引入其中的宿主生物例如细菌或植物。核酸分子可以被稳定地整合到宿主的基因组中，或者核酸分子还可以作为染色体外分子存在。这种染色体外分子能够自主复制。转化的细胞、组织或植物应当理解为不仅涵盖转化过程的终产物，而且涵盖其转基因子代。“非转化的”、“非转基因的”、或“非重组的”宿主是指不含该异源的核酸分子的野生型生物，例如细菌或植物。
本发明总体上涉及调节例如降低重组杀昆虫蛋白在转基因植物的雄性生殖细胞和/或组织中的表达的方法，可用于此类方法中的重组DNA构建体，以及含有此类重组DNA构建体的转基因植物、植物部分、细胞和种子。重组DNA构建体和含有此类构建体的转基因植物、植物部分、细胞和种子提供了一种大大改进的方法，以最小化对与花粉中杀昆虫蛋白的表达相关的非有害生物昆虫物种的任何潜在风险，或用于减轻花粉和/或绒毡层中杀昆虫蛋白的表达可能对转基因植物雄性能育性产生的影响。
在一些实施例中，本发明提供了包含杀昆虫蛋白编码序列的重组DNA构建体，该杀昆虫蛋白编码序列可操作地连接至编码微小RNA(miRNA)元件的DNA序列，该miRNA元件能够结合至植物微小RNA，该植物微小RNA在玉米植物中是雄性组织特异性的或雄性组织优选的，即本发明提供了嵌合转基因，该嵌合转基因包括编码重组杀昆虫蛋白的杀昆虫蛋白编码序列和至少一种miRNA元件，该miRNA元件包含至少一个可操作地连接至蛋白质编码序列的雄性组织特异性或雄性组织优选的miRNA起始子序列。在其他实施例中，重组DNA构建体可用于抑制重组杀昆虫蛋白在转基因植物(如转基因玉米植物)的雄性生殖组织中的表达。在其他实施例中，本发明提供了包含重组DNA构建体的重组DNA分子及其使用方法。如所指定的，本发明的核酸序列可以DNA或RNA的形式提供；如本领域普通技术人员所熟知的，披露其中一种必然限定另一种。此外，如本领域普通技术人员所熟知的，给定核酸序列的披露必然限定该序列的精确互补体。
“雄性组织特异性”或“雄性组织优选的”miRNA起始子序列是约18至约26个核苷酸(例如，18、19、20、21、22、23、24、25或26个核苷酸)的小RNA，该miRNA起始子序列被内源性植物微小RNA识别并结合，该内源性植物微小RNA在植物的一种或多种雄性生殖组织(例如，花粉和/或绒毡层)中富集(雄性组织优选的)或特异性表达(雄性组织特异性)，即，具有雄性组织特异性或雄性组织优选的表达模式。雄性组织特异性或雄性组织优选的miRNA在植物中天然存在，并且可以使用本领域已知的技术(如低分子量northern分析)进行检测。内源性玉米植物微小RNA的miRNA起始子序列的实例如SEQ ID NO:29-34和SEQ ID NO:94-97所示。编码此miRN起始子序列的DNA序列的实例分别如SEQ ID NO:41-46和SEQ ID NO:98-101所示。在一些实施例中，miRNA起始子序列是给定的雄性组织特异性或雄性组织优选的微小RNA的精确DNA互补体(无错配)。这样的miRNA起始子序列在本文中被称为“基序”序列。在其他实施例中，miRNA起始子序列与给定的雄性组织特异性或雄性组织优选的微小RNA至少有1-3个核苷酸错配的变化，这些miRNA起始子序列在本文中被称为“t基因”序列，因为该起始子序列的设计基于内源性靶基因的预测序列。然而，这些“t基因”miRNA起始子序列具有足够的互补性，以例如在典型的生理条件下与预期的微小RNA结合或杂交。“互补性”是指一条多核苷酸链上的核苷酸与另一条多核苷酸链上的核苷酸根据标准沃森-克里克互补法则进行碱基配对的能力(即，鸟嘌呤与胞嘧啶(G:C)配对，并且腺嘌呤与胸腺嘧啶(A:T)或尿嘧啶(A:U)配对)；链内杂交可能发生在单个多核苷酸的两个或更多个互补区之间。当包括在如本文所述的重组DNA构建体中时，miRNA起始子序列能够进行RNAi介导的抑制或者能够破坏转基因RNA的表达或重组蛋白(如重组杀昆虫蛋白)的表达。
在本发明的一些实施例中，本发明的微小RNA和本发明的miRNA起始子序列在花粉组织或绒毡层组织中选择性地或优选地发挥作用。
在本发明的一些实施例中，本发明的DNA构建体包含杀昆虫蛋白编码序列的5’非翻译区内的miRNA元件。在其他实施例中，本发明的DNA构建体包含杀昆虫蛋白编码序列的3’非翻译区内的miRNA元件。在仍其他实施例中，本发明的DNA构建体包含起始和终止密码子之间的杀昆虫蛋白编码序列内的miRNA元件。在其他实施例中，miRNA元件位于蛋白质编码序列与作为3'非翻译区的一部分的聚腺苷酸化序列之间。
在本发明的一些实施例中，本发明的DNA构建体包含至少一个、至少两个、至少三个、或超过三个miRNA元件。miRNA元件中的每一个可以包含至少一个、至少两个、至少三个、或超过三个miRNA起始子序列。miRNA元件可以是任何长度，但优选地，它们是从约40个核苷酸至约80个核苷酸。在其他实施例中，本发明的miRNA元件包含编码miRNA起始子序列的序列，该miRNA起始子序列选自由SEQ ID NO:29-34或SEQ ID NO:94-97组成的组。在其他实施例中，编码本发明的miRNA起始子序列的序列选自由SEQ ID NO:41-46或SEQ ID NO:98-101组成的组。在仍其他实施例中，miRNA元件包含靶插入序列，该靶插入序列包含miRNA起始子序列和该起始子序列的5`和/或3`末端侧翼的合成核苷酸序列。这种侧翼序列可用于使微小RNA更好地接近miRNA起始子序列。在起始子序列的5’末端，侧翼大小可以为约40、或约30、或约20、19、18、17、16或15个核苷酸，但优选17个核苷酸。在起始子序列的3’末端，侧翼大小可以在约40、或约30、或约20、19、18、17、16、15、13、12或11个核苷酸的范围内，但优选13个核苷酸。在其他实施例中，包含本发明的起始子序列和该起始子序列的5’和/或3’末端上的合成侧翼序列的序列是本发明的靶插入序列。在其他实施例中，靶插入序列选自由SEQID NO:10-18或SEQ ID NO:79-83组成的组。因此，本发明的miRNA元件可以仅包含miRNA起始子序列，或可以包含靶插入序列及其任何组合。
在一些实施例中，本发明的miRNA元件在体外合成或修饰，以含有更多、更少或不同的miRNA起始子序列或侧翼序列，和/或以重排一个或多个miRNA起始子序列或侧翼序列的相对位置，其中这种修改有利于增加或减少该miRNA元件的作用。用于合成或体外修饰miRNA元件并确定所希望的抑制水平的最佳变化的方法是本领域技术人员已知的。还可以使用本领域技术人员已知的方法设计嵌合miRNA元件，例如通过在miRNA元件内部插入另外的所希望的miRNA起始子序列，或者通过将另外的miRNA起始子序列5'或3'连接到miRNA元件。
在一些实施例中，与转基因玉米中的非雄性生殖组织(如叶组织)相比，miRNA起始子序列在转基因玉米植物中的表达降低了本发明的重组杀昆虫蛋白在该转基因玉米植物的雄性生殖组织(如花粉和/或绒毡层)中的表达。如本文所使用的，细胞或组织中重组杀昆虫蛋白表达的降低是指细胞或组织中重组杀昆虫蛋白的水平与参考细胞或组织相比降低或抑制至少约25％、至少约35、至少约45％、至少约55％、至少约65％、至少约75％、至少约80％、至少约85％、至少约90％、至少约95％、或至少约99％。参考细胞或组织可以是例如来自表达重组杀昆虫蛋白的相同或相似的转基因植物的营养细胞或组织，或例如来自具有表达重组杀昆虫蛋白的相似的转基因但缺乏miRNA元件的转基因植物的营养细胞或组织。可以使用本领域技术人员已知的任何技术确定杀昆虫蛋白表达的降低，例如通过使用如ELISA或蛋白质印迹分析等技术直接测量细胞或组织样品中的蛋白质积累，通过测量杀昆虫蛋白的生物活性，或通过表型确定杀昆虫蛋白表达。在一些实施例中，重组杀昆虫蛋白的减少是指重组杀昆虫蛋白在转基因玉米植物的雄性生殖组织中的表达充分降低，这产生与合适的对照玉米植物相比转基因玉米植物中增加的雄性能育性的可检测表型。因此，在这种转基因玉米植物中检测到增加的雄性能育性将表明重组杀昆虫蛋白的选择性减少。
在其他实施例中，与转基因玉米中的非雄性生殖组织(如叶组织)相比，本发明的miRNA起始子序列在转基因玉米植物中的表达增加了本发明的重组杀昆虫蛋白在该转基因玉米植物的雄性生殖组织(如花粉或绒毡层)中的表达。如本文所使用的，细胞或组织中重组杀昆虫蛋白表达增加是指细胞或组织中重组杀昆虫蛋白的水平与参考细胞或组织相比增加至少约25％、至少约35、至少约45％、至少约55％、至少约65％、至少约75％、至少约80％、至少约85％、至少约90％、至少约95％、或至少约99％。参考细胞或组织可以是例如来自表达重组杀昆虫蛋白的相同或相似的转基因植物的营养细胞或组织，或例如来自具有表达重组杀昆虫蛋白的相似的转基因但缺乏miRNA元件的转基因植物的营养细胞或组织。可以使用本领域技术人员已知的任何技术确定杀昆虫蛋白表达的增加，例如通过使用如ELISA或蛋白质印迹分析等技术直接测量细胞或组织样品中的蛋白质积累，通过测量杀昆虫蛋白的生物活性，或通过表型确定杀昆虫蛋白表达。
在一些实施例中，由本发明的DNA构建体编码的重组杀昆虫蛋白在转基因玉米植物中的表达赋予了转基因玉米植物至少在营养组织中的昆虫有害生物耐受性。这种耐受性是由杀昆虫蛋白导致昆虫有害生物的摄食或生长减少或者导致昆虫有害生物的死亡引起的。在一些实施例中，本发明的DNA构建体编码重组Cry1杀昆虫蛋白或重组Vip3杀昆虫蛋白。在其他实施例中，Cry1蛋白是Cry1A杀昆虫蛋白，或Vip3蛋白是Vip3A杀昆虫蛋白。在仍其他实施例中，Cry1A蛋白是Cry1Ab杀昆虫蛋白，或Vip3A蛋白是Vip3Aa杀昆虫蛋白。在仍另外的实施例中，Cry1Ab杀昆虫蛋白包含选自由SEQ ID NO:61-66组成的组的氨基酸序列。在仍另外的实施例中，Vip3Aa蛋白包含选自由SEQ ID NO:115-117组成的组的氨基酸序列。
在一些实施例中，本发明的重组DNA构建体包含表达盒，该表达盒包含可操作地连接至重组杀昆虫蛋白编码序列的异源启动子，该重组杀昆虫蛋白编码序列可操作地连接至编码本发明的微小RNA(miRNA)元件的DNA序列，该miRNA元件在玉米植物中是雄性组织特异性的或雄性组织优选的。在一个实施例中，表达盒包含选自由SEQ ID NO:20-28或SEQ IDNO:85-93组成的组的序列。
在一些实施例中，本发明提供了包含本发明的DNA构建体的重组载体。在其他实施例中，载体包含选自由SEQ ID NO:49-59或SEQ ID NO:105-113组成的组的序列。
在一些实施例中，本发明提供了包含本发明的DNA构建体和/或表达盒和/或重组载体的转基因玉米(玉蜀黍(maize/Zea mays))植物。在其他实施例中，本发明提供了本发明的转基因玉米植物的转基因种子、子代或植物部分，其中该转基因种子、子代或植物部分包含本发明的DNA构建体、表达盒或重组载体。
在一些实施例中，本发明提供了制备DNA构建体的方法，该方法包括鉴定内源性雄性组织特异性或雄性组织优选的玉米微小RNA(miRNA)；构建编码至少一个被所述玉米miRNA识别的miRNA起始子序列的miRNA元件，以及将所述miRNA元件可操作地连接至杀昆虫蛋白编码序列。在其他实施例中，雄性组织特异性微小RNA或雄性组织优选的微小RNA是花粉特异性或花粉优选的或者是绒毡层特异性或绒毡层优选的。在其他实施例中，雄性组织特异性miRNA元件或雄性组织优选的miRNA元件是花粉特异性或花粉优选的或者是绒毡层特异性或绒毡层优选的。在其他实施例中，miRNA元件包含靶插入序列。在仍其他实施例中，靶插入序列包含选自由SEQ ID NO:10-18或SEQ ID NO:79-83组成的组的序列。在仍另外的实施例中，由所述miRNA元件编码的miRNA起始子序列包含选自由SEQ ID NO:29-34或SEQID NO:94-97组成的组的序列。
在一些实施例中，本发明提供了降低重组杀昆虫蛋白在转基因玉米植物的雄性生殖组织中的表达的方法，该方法包括在该转基因玉米植物中表达DNA构建体，该DNA构建体包含编码重组杀昆虫蛋白的杀昆虫蛋白编码序列和可操作地连接至该杀昆虫蛋白编码序列的至少第一雄性组织特异性miRNA元件或雄性组织优选的miRNA元件，其中该miRNA元件编码至少一个miRNA起始子序列。在其他实施例中，miRNA元件包含在杀昆虫蛋白编码序列的5’非翻译区内、或杀昆虫蛋白编码序列的3’非翻译区内、或起始和终止密码子之间的杀昆虫蛋白编码序列内。在其他实施例中，miRNA元件包含至少两个、至少三个、或超过三个miRNA起始子序列。在仍其他实施例中，雄性组织是花粉和/或绒毡层。在仍其他实施例中，miRNA起始子序列选自由SEQ ID NO:29-34或SEQ ID NO:94-97组成的组。在仍其他实施例中，miRNA元件包含选自由SEQ ID NO:10-18或SEQ ID NO:79-83组成的组的靶插入序列。
在选择性降低重组杀昆虫蛋白在转基因玉米植物的雄性生殖组织中的表达的方法的一些实施例中，杀昆虫蛋白在转基因玉米植物中的表达赋予了玉米植物至少在植物的营养组织(如叶组织)中的昆虫有害生物耐受性。在其他实施例中，杀昆虫蛋白编码序列编码Cry1蛋白或Vip3蛋白。在其他实施例中，杀昆虫蛋白编码序列编码Cry1A蛋白或Vip3A蛋白。在仍其他实施例中，杀昆虫蛋白编码序列编码Cry1Ab蛋白或Vip3Aa蛋白。在仍其他实施例中，杀昆虫蛋白编码序列编码Cry1Ab蛋白，该Cry1Ab蛋白包含选自由SEQ ID NO:61-66组成的组的氨基酸序列。在其他实施例中，杀昆虫蛋白编码序列编码Vip3Aa蛋白，该Vip3Aa蛋白包含选自由SEQ ID NO:115-117组成的组的氨基酸序列。在仍其他实施例中，DNA构建体包含表达盒，该表达盒包含选自由SEQ ID NO:20-28或SEQ ID NO:85-93组成的组的序列。
在一些实施例中，本发明提供了产生具有增加的雄性能育性的表达Vip3的玉米植物的方法，该方法包括向玉米植物部分的基因组中引入重组DNA构建体，该重组DNA构建体包含可操作地连接至编码微小RNA(miRNA)元件的DNA序列的Vip3蛋白编码序列，该miRNA元件在玉米植物中是雄性组织特异性的或雄性组织优选的(如绒毡层特异性或绒毡层优选的)，其中该miRNA元件相对于该Vip3蛋白编码序列是异源的；由该玉米植物部分产生玉米植物，其中miRNA元件降低Vip3蛋白在雄性生殖组织(例如绒毡层)中的表达，从而产生与没有DNA构建体的表达Vip3的玉米植物相比具有增加的雄性能育性的表达Vip3的玉米植物。
在一些实施例中，本发明提供了产生具有增加的雄性能育性的表达Vip3的玉米植物的方法，该方法包括：(a)使表达Vip3的第一玉米植物与第二玉米植物杂交，其中该第一玉米植物在其基因组中包含重组DNA构建体，该重组DNA构建体包含可操作地连接至编码微小RNA(miRNA)元件的DNA序列的Vip3蛋白编码序列，该miRNA元件在玉米植物中是雄性组织特异性的或雄性组织优选的，其中该miRNA元件相对于该Vip3蛋白编码序列是异源的；以及任选地(b)使步骤(a)中所得的包含DNA构建体的表达Vip3的子代玉米植物与亲本植物回交，以产生回交子代植物；(c)选择包含DNA构建体的表达Vip3的子代植物进行回交；以及(d)进行步骤b)和c)至少三次以将DNA构建体固定在所希望的遗传背景中，从而产生包含该DNA构建体并且与不含该DNA构建体的表达Vip3的玉蜀黍植物相比具有增加的雄性能育性的表达Vip3的玉米植物。
在一些实施例中，本发明提供了提高表达Vip3的玉米植物的种子产量的方法，该方法包括：(a)使第一玉米植物或玉米种质与第二玉米植物或玉米种质杂交，其中该第一或第二玉米植物或玉米种质表达Vip3杀昆虫蛋白，并且其中该第一玉米植物或玉米种质在其基因组中包含重组DNA构建体，该重组DNA构建体包含可操作地连接至编码微小RNA(miRNA)元件的DNA序列的Vip3蛋白编码序列，该miRNA元件在玉米植物中是雄性组织特异性的或雄性组织优选的，其中该miRNA元件相对于该Vip3蛋白编码序列是异源的，并且其中该miRNA元件降低该Vip3蛋白在该雄性生殖组织中的表达水平；以及(b)使用包含该DNA构建体的子代玉蜀黍植物作为传粉者与其自身或与作为种子亲本起作用的第二玉米植物或玉米种质杂交，从而与合适的对照杂交相比提高杂交的种子产量。
在上述用于产生表达Vip3的玉米植物以及提高表达Vip3的玉米植物的种子产量的方法的一些实施例中，miRNA元件包含在所述杀昆虫蛋白编码序列的5’非翻译区内、或杀昆虫蛋白编码序列的3’非翻译区内、或起始和终止密码子之间的杀昆虫蛋白编码序列内。在其他实施例中，雄性组织是花粉和/或绒毡层。在其他实施例中，miRNA元件包含编码至少一个miRNA起始子序列的核苷酸序列。在仍其他实施例中，由核苷酸序列编码的miRNA起始子序列选自由SEQ ID NO:94-97组成的组。在这些方法的仍其他实施例中，与转基因玉米植物中的非雄性生殖组织相比，miRNA起始子序列在转基因玉米植物中的表达降低了Vip3蛋白在该转基因玉米植物的雄性生殖组织中的表达。在另外的实施例中，miRNA元件包含选自由SEQ ID NO:74-78组成的组的序列。
在上述本发明的方法的一些实施例中，Vip3蛋白编码序列在转基因玉米植物中的表达赋予了该玉米植物对昆虫有害生物的耐受性。在其他实施例中，昆虫有害生物是秋夜蛾或玉米穗蛾。在其他实施例中，Vip3蛋白是Vip3A蛋白。在仍其他实施例中，Vip3A蛋白是Vip3Aa蛋白。在仍其他实施例中，Vip3Aa蛋白包含选自由SEQ ID NO:115-117组成的组的氨基酸序列。在这些方法的仍其他实施例中，DNA构建体包含表达盒，其中该表达盒包含选自由SEQ ID NO:85-93组成的组的序列。
在一些实施例中，本发明提供了重组杀昆虫蛋白，该重组杀昆虫蛋白对鳞翅目昆虫有活性，其中该杀昆虫蛋白包含由雄性组织特异性或雄性组织优选的miRNA元件编码的氨基酸序列，并且其中该miRNA元件编码至少一个miRNA起始子序列。在其他实施例中，杀昆虫蛋白是Cry1蛋白或Vip3蛋白。在其他实施例中，Cry1蛋白是Cry1A蛋白，或Vip3蛋白是Vip3A蛋白。在仍其他实施例中，Cry1A蛋白是Cry1Ab蛋白，并且Vip3A蛋白是Vip3Aa蛋白。在仍其他实施例中，氨基酸序列选自由SEQ ID NO:61-66或SEQ ID NO:115-117组成的组。在其他实施例中，重组杀昆虫蛋白对鳞翅目有害生物有活性，该鳞翅目有害生物是欧洲玉米螟、秋夜蛾或玉米穗蛾。在其他实施例中，雄性组织是花粉或绒毡层。在其他实施例中，miRNA元件包含靶插入序列。在仍其他实施例中，miRNA元件包含选自由SEQ ID NO:10-18或SEQ ID NO:74-78组成的组的靶插入序列。在仍其他实施例中，miRNA起始子序列选自由SEQID NO:29-34或SEQ ID NO:94-97组成的组。
在一些实施例中，本发明提供了编码本发明的重组杀昆虫蛋白的合成多核苷酸。在其他实施例中，合成多核苷酸包含选自由SEQ ID NO:68-73或SEQ ID NO:118-121组成的组的序列。
在一些实施例中，在转基因植物中表达本发明的DNA构建体，因此引起相应的重组杀昆虫蛋白在该转基因植物中的生物合成。以这种方式，产生了对昆虫(例如鳞翅目昆虫有害生物)具有增强的抗性的转基因植物。为了其在转基因植物中的表达，可对本发明的DNA构建体任选地进行修饰和优化。尽管在许多情况中来自微生物有机体的基因可以在没有修饰的情况下在植物中以高水平表达，但在转基因植物中的低表达可能是由于具有在植物中不优选的密码子的微生物核酸所造成的。在本领域中已知的是，所有生物针对密码子使用具有特定偏好，并且可以对本发明中所描述的核酸的密码子进行改变以符合植物的偏好，同时保持由此所编码的氨基酸。此外，在植物中高表达最好是由以下编码序列实现的，这些编码序列具有至少约35％、优选大于约45％、更优选大于约50％、并且最优选大于约60％的GC含量。具有低GC含量的微生物核酸可能在植物中表达欠佳，这是由于存在可使信息不稳定的ATTTA基序，以及可能引起不适当的聚腺苷酸化作用的AATAAA基序。在一些实施例中，可以对序列进行修饰以便迎合单子叶植物或双子叶植物的特定密码子偏好以及GC含量偏好，因为这些偏好已经被证明是不同的(Murray等人Nucl.Acids Res.[核酸研究]17:477-498(1989))。此外，针对可能引起信息缩短的不合理剪接位点的存在对这些核酸筛选。可以使用熟知的位点定向诱变、PCR、以及合成基因的构建的技术，例如，使用公开的专利申请EP0 385 962、EP 0 359 472、以及WO 93/07278中所述的方法，对在这些核酸之内所有需要做出的变化(如以上所描述的那些变化)进行改变。
在本发明的一些实施例中，根据美国专利5,625,136(通过引用并入本文)中所披露的程序来制造本发明的杀昆虫蛋白的编码序列。在这个程序中，使用了玉蜀黍优选的密码子，即最频繁地编码玉蜀黍中的氨基酸的单个密码子。针对特定的氨基酸的玉蜀黍优选的密码子可例如源自玉蜀黍的已知基因序列。针对来自玉蜀黍植物的28个基因的玉蜀黍密码子使用发现于Murray等人,Nucleic Acids Research[核酸序列]17:477-498(1989)中，其披露内容通过引用并入本文。
以这种方式，这些核苷酸序列可以进行优化用于在任何植物中表达。认识到的是该基因序列的所有或任何部分可以是优化的或合成的。即，还可以使用合成的或部分优化的序列。
为了更加有效的翻译起始，可修饰与起始甲硫氨酸相邻的序列。例如，它们可以通过包含已知在植物中有效的序列而被修饰。Joshi已经提出了针对植物的适当的共有序列(NAR 15:6643-6653(1987))，并且Clonetech提出了另一种共有翻译起始子(1993/1994目录，第210页)。这些共有序列适合与本发明的核酸一起使用。在实施例中，将这些序列掺入包含核酸的构建体中，达到并且包括ATG(而未对第二个氨基酸进行修饰)，或者可替代地达到并且包括在ATG后的GTC(具有修饰该转基因的第二氨基酸的可能性)。
本发明的核酸分子在转基因植物中的表达是由在植物中起作用的启动子驱动的。启动子的选择将根据表达的时间和空间需要而变化，并且还根据靶物种而变化。因此，本发明的核酸在叶、柄(stalk)或茎(stem)、穗、花序(例如穗状花序、圆锥花序、穗轴等等)、根、和/或籽苗中的表达是优选的。然而在许多情况下，寻求针对多于一种类型昆虫有害生物的保护，并且因此在多个组织中的表达是令人希望的。尽管已经显示来自双子叶植物的很多启动子在单子叶植物中是可操作的并且反之亦然，但理想的是选择双子叶植物启动子用于在双子叶植物中表达，并且选择单子叶植物启动子用于在单子叶植物中表达。不过，对于所选的启动子的起源没有限制；只要它们可有效驱动核酸在所希望的细胞中表达就足够了。
在一些实施例中，使用了以组成型表达的启动子，包括肌动蛋白或泛素或cmp启动子，或CaMV 35S和19S启动子。本发明的核酸也可以在用化学方法进行调节的启动子的调节下表达。用于基因表达的化学诱导的优选的技术详述于公开申请EP 0 332 104(汽巴-嘉基公司(Ciba-Geigy))以及美国专利5,614,395中。用于化学诱导的优选的启动子是烟草PR-1a启动子。
在其他实施例中，可使用一类伤口诱导型启动子。已经描述了数量众多的在创伤部位并且还在植物病原菌感染的部位表达的启动子。理想的是，这种启动子应该仅在感染的部位有局部活性，并且以这种方式，本发明的嵌合杀昆虫蛋白仅在需要合成这些蛋白的细胞中累积以杀死入侵的昆虫有害生物。这类优选的启动子包括由以下文献所描述的那些：Stanford等人Mol.Gen.Genet.[分子遗传学]215:200-208(1989)，Xu等人PlantMolec.Biol.[植物分子生物学]22:573-588(1993)，Logemann等人Plant Cell[植物细胞]1:151-158(1989)，Rohrmeier和Lehle,Plant Molec.Biol.[植物分子生物学]22:783-792(1993)，Firek等人Plant Molec.Biol.[植物分子生物学]22:129-142(1993)，以及Warner等人Plant J.[植物杂志]3:191-201(1993)。
用于在植物(特别是玉米)中表达对本发明的嵌合杀昆虫蛋白进行编码的基因的组织特异性的或组织优选的启动子是那些直接在根、髓、叶或花粉(特别是根)中表达的启动子。此类启动子，例如从PEPC或trpA中分离的那些披露于美国专利号5,625,136中，或从MTL中分离的那些披露于美国专利号5,466,785中。这两篇美国专利以其全文通过引用并入本文。在一些实施例中，雄性组织特异性或雄性组织优选的启动子与本发明的DNA构建体(即，包含可操作地连接至编码微小RNA(miRNA)元件的DNA序列的杀昆虫蛋白编码序列的DNA构建体，该miRNA元件在玉米植物中是雄性组织特异性的或雄性组织优选的，其中所述miRNA元件相对于所述杀昆虫蛋白编码序列是异源的)的组合导致杀昆虫蛋白在花粉或绒毡层中的表达的下降比单独归因于组织特异性/组织优选的启动子或miRNA元件的下降更大。
此外，可以使用在质体中发挥作用的启动子。此类启动子的非限制性实例包括噬菌体T3基因9 5'UTR以及其他的披露于美国专利号7,579,516中的启动子。适用于本发明的其他启动子包括但不限于S-E9小亚基RuBP羧化酶启动子和Kunitz胰蛋白酶抑制剂基因启动子(Kti3)。
在本发明的一些实施例中，可以使用诱导型启动子。因此，例如，可以使用化学调节型启动子以通过应用外源化学调节物来调节本发明的核苷酸序列的表达。本发明的核苷酸序列的表达经由化学调节过的启动子进行的调节使得本发明的多肽仅当用诱导的化学物处理作物植物时能被合成。取决于目的，在应用化学品诱导本发明的核苷酸序列的表达时，该启动子可以是化学诱导型启动子，或者在应用化学品抑制本发明的核苷酸序列表达时该启动子可以是化学阻抑型启动子。
化学诱导型启动子在本领域是已知的，并且包括但不限于玉蜀黍In2-2启动子(其由苯磺酰胺除草剂安全剂激活)、玉蜀黍GST启动子(其由用作发芽前除草剂的疏水亲电子化合物激活)、以及烟草PR-1a启动子(其由水杨酸激活)(例如，PR1a系统)、类固醇反应性启动子(参见例如，Schena等人(1991)Proc.Natl.Acad.Sci.[美国国家科学院院刊]USA 88,10421-10425和McNellis等人(1998)Plant J.[植物杂志]14,247-257中的糖皮质激素诱导型启动子)以及四环素诱导型启动子和四环素-阻抑型启动子(参见例如，Gatz等人(1991)Mol.Gen.Genet.[分子遗传学]227,229-237和美国专利号5,814,618和5,789,156)、Lac阻遏物系统启动子、铜诱导型系统启动子、水杨酸诱导型系统启动子(例如，PR1a系统)、糖皮质激素诱导型启动子(Aoyama等人(1997)Plant J.[植物杂志]11:605-612)以及蜕皮激素诱导型系统启动子。
诱导型启动子的其他非限制性实例包括ABA诱导型和细胞膨胀诱导型启动子、植物生长素结合蛋白基因启动子(Schwob等人(1993)Plant J.[植物杂志]4:423-432)、UDP葡萄糖类黄酮糖基转移酶启动子(Ralston等人(1988)Genetics[遗传]119:185-197)、MPI蛋白酶抑制剂启动子(Cordero等人(1994)Plant J.[植物杂志]6:141-150)以及甘油醛-3-磷酸脱氢酶启动子(Kohler等人(1995)Plant Mol.Biol.[植物分子生物学]29:1293-1298；Martinez等人(1989)J.Mol.Biol.[分子生物学杂志]208:551-565；和Quigley等人(1989)J.Mol.Evol.[分子进化杂志]29:412-421)。还包括苯磺酰胺诱导型(美国专利号5,364,780)和乙醇诱导型(国际专利申请公开号WO 97/06269和WO 97/06268)系统和谷胱甘肽S-转移酶启动子。同样地，可以使用描述于以下文献中的诱导型启动子中的任一种：Gatz(1996)Current Opinion Biotechnol.[生物技术新见]7:168-172和Gatz(1997)Annu.Rev.Plant Physiol.Plant Mol.Biol.[植物生理学与植物分子生物学年度综述]48:89-108。适用于指导本发明的核苷酸序列在植物中的表达的其他化学诱导型启动子披露于美国专利5,614,395中，该专利通过引用以其全文并入本文。基因表达的化学诱导还详述于公开申请EP 0 332 104(授予汽巴-嘉基公司)和美国专利5,614,395中。在一些实施例中，用于化学诱导的启动子可以是烟草PR-1a启动子。
在另外的方面中，本发明的核苷酸序列可以与启动子可操作地相关联，该启动子是创伤诱导型或由有害生物或病原体侵染(例如，昆虫或线虫植物有害生物)进行的诱导型。已经描述了在创伤部位和/或在有害生物攻击(例如，昆虫/线虫摄食)或植物病原菌侵染的部位处表达的数量众多的启动子。理想的是，这种启动子应仅在攻击部位处或邻近该部位具有局部活性，并且以这种方式，本发明的核苷酸序列的表达将集中在所侵入或摄食的细胞中。此类启动子包括但不限于由以下描述的那些启动子：Stanford等人,Mol.Gen.Genet.[分子与普通遗传学]215:200-208(1989)；Xu等人,Plant Molec.Biol.[植物分子生物学]22:573-588(1993)；Logemann等人,Plant Cell[植物细胞]1:151-158(1989)；Rohrmeier和Lehle，Plant Molec.Biol.[植物分子生物学]22:783-792(1993)；Firek等人,Plant Molec.Biol.[植物分子生物学]22:129-142(1993)；Warner等人,PlantJ.[植物杂志]3:191-201(1993)；美国专利号5,750,386；美国专利号5,955,646；美国专利号6,262,344；美国专利号6,395,963；美国专利号6,703,541；美国专利号7,078,589；美国专利号7,196,247；美国专利号7,223,901；以及美国专利申请公开2010043102。
在本发明的一些实施例中，使用“最小启动子”或“基本启动子”。最小启动子能够募集并结合RNA聚合酶II复合体及其辅助蛋白，以允许转录起始和延伸。在一些实施例中，最小启动子被构建成仅包含来自转录因子的结合和目的核苷酸序列的转录所必需的选定启动子的核苷酸/核苷酸序列的启动子，这一目的核苷酸序列可操作地与包括但不限于TATA盒序列的最小启动子相关联。在其他实施例中，最小启动子缺少募集并结合转录因子的cis序列，这些转录因子调节(例如，增强、阻抑、赋予组织特异性，赋予诱导性或阻抑性)转录。最小启动子通常被放置在待表达的核苷酸序列的上游(即，5’)。因此，可以选择来自与本发明一起可用的任何启动子的核苷酸/核苷酸序列用作最小启动子。
可以将数量众多的其他序列掺入本发明所描述的表达盒中。这些序列包括已经显示出增强表达的序列，如内含子序列(例如，来自Adhl和bronzel)以及病毒的前导序列(例如，来自TMV、MCMV、和AMV)。
本发明的核酸在植物中针对不同的细胞定位的靶向表达可能是更优选的。在一些情况下，在胞质溶胶中的定位可能是令人希望的，而在其他情况下，在某个亚细胞器中的定位可能是优选的。使用本领域内熟知的技术进行编码酶类的转基因的亚细胞定位。典型地，对编码来自已知细胞器靶向的基因产物的靶肽的DNA进行操作并将其融合在该核酸的上游。针对叶绿体的许多此类靶序列是已知的并且已经证明了它们在异源构建体中的功能。还将本发明的核酸的表达靶向至宿主细胞的内质网或液泡。实现其的技术在本领域中是熟知的。
适合于植物转化的载体描述于在本说明书的其他地方。对于农杆菌介导的转化，二元载体或携带至少一种T-DNA边界序列的载体是合适的，而对于直接基因转移，任何载体都是合适的，并且仅含有目的构建体的线性DNA也许是优选的。在直接基因转移的情况下，可以使用以单个DNA种类的转化或共转化(Schocher等人,Biotechnology[生物技术]4:1093-1096(1986))。对于直接基因转移以及农杆菌介导的转化这两者，转化通常(但不是必需的)用选择性标记进行，这种选择性标记可以提供针对抗生素(卡那霉素、潮霉素、或甲氨蝶呤)或除草剂(basta)的抗性。包含本发明的核酸分子的植物转化载体还可包含以下基因(例如磷酸甘露糖异构酶；PMI)，这些基因提供转基因植物的阳性选择，如美国专利5,767,378和5,994,629(通过引用并入本文)中所披露的。然而，选择性标记的选择对于本发明并不是至关重要的。
在一些实施例中，核酸可以转化到核基因组中。在其他实施例中，本发明的核酸被直接转化到质体基因组中。质体转化的主要优点在于质体通常能够表达细菌基因而无需实质性的密码子优化，而且质体能够在单一启动子的控制下表达多个开放阅读框。在美国专利号5,451,513、5,545,817和5,545,818中，在PCT申请号WO 95/16783中，以及在McBride等人,(1994),Proc.Nati.Acad.Sci.USA[美国国家科学院院刊]91,7301-7305中广泛描述了质体转化技术。基本的叶绿体转化技术涉及例如使用生物射弹(biolistic)或原生质体转化(例如，氯化钙或PEG介导的转化)，将位于选择性标记侧翼的经克隆的质体DNA区连同目的基因一起引入合适的靶组织中。这些1至1.5kb的侧翼区(被命名为靶向序列)促进了与质体基因组的同源重组，并且因而允许置换或修饰原质体(plastome)的特定区域。最初，将叶绿体16S rRNA和rps12基因的点突变(赋予对壮观霉素和/或链霉素抗性)用作用于转化的选择性标记(Svab,Z.,Hajdukiewicz,P.,和Maliga,P.(1990)Proc.Nati.Acad.Sci.USA[美国国家科学院院刊]87,8526-8530；Staub,J.M.,和Maliga,P.(1992)Plant Cell[植物细胞]4,39-45)。这以大约每100次靶叶轰击大约1个的频率产生稳定的同质转化株。在这些标记之间克隆位点的存在允许建立质体靶向载体用于外来基因的引入(Staub,J.M.和Maliga,P.(1993)EMBO J.[欧洲分子生物学杂志]12,601-606)。转化频率的实质性增加是通过用显性选择性标记(细菌aadA基因，其编码了壮观霉素-解毒酶氨基糖苷-3'-腺苷酰转移酶)置换隐性rRNA或r-蛋白抗生素抗性基因来获得的(Svab,Z.和Maliga,P.(1993)Proc.Natl.Acad.Sci.USA[美国国家科学院院刊]90,913-917)。先前，这种标记已经被成功地用于莱茵衣藻(Chlamydomonas reinhardtii)这种绿藻的质体基因组的高频率转化(Goldschmidt-Clermont,M.(1991)Nucl.Acids Res.[核酸研究]19:4083-4089)。有用于质体转化的其他选择性标记在本领域是已知的，并且被包括在本发明的范围之内。典型地，转化之后需要大约15-20个细胞分裂循环以便达到同质状态。质体表达(其中基因通过同源重组被插入到在每个植物细胞中存在的所有数千个环状质体基因组的拷贝中)利用了超过核表达的基因的庞大的拷贝数目的优点，以便允许能够很容易超过总的可溶性植物蛋白的10％的表达水平。在一个优选的实施例中，将本发明的核酸插入到质体靶向的载体中并且转化到所希望的植物宿主的质体基因组中。获得了对于包含本发明的核酸的质体基因组同型的植物，并且这些植物能够优先地高表达该核酸。
在一些实施例中，本发明的转基因植物可包含至少一种非蛋白质的第二杀有害生物剂。在这些实施例的一些方面，该第二杀有害生物剂是干扰RNA分子。干扰RNA分子典型地包含针对靶基因的至少一种RNA片段、间隔序列、和与第一RNA片段互补的第二RNA片段，从而可以形成双链RNA结构。当生物识别双链RNA(dsRNA)分子并水解它们时，RNA干扰(RNAi)发生。所得的水解产物是约19-24个核苷酸长度的小RNA片段，这些小RNA片段被称为小干扰RNA(siRNA)。然后这些siRNA扩散或被携带至整个生物，包括越过细胞膜，在那里它们与mRNA(或其他的RNA)杂交并且导致RNA的水解。干扰RNA由RNA干扰沉默复合体(RISC)识别，RNA的效应链(或“引导链”)位于所述复合体中。此引导链充当双链体序列的识别和破坏模板。每次siRNA与其互补RNA靶标杂交，此过程都被重复，有效防止那些mRNA被翻译，并且因此“沉默”mRNA自其中转录的特异性基因的表达。本领域已知干扰RNA可用于昆虫控制(参见例如，公开物WO 2013/192256，其通过引用并入本文)。设计用于昆虫控制的干扰RNA产生非天然存在的双链RNA，其利用昆虫中的天然RNAi途径来触发靶基因的下调，这可能导致停止摄食和/或生长并可能导致昆虫有害生物死亡。干扰RNA分子可赋予针对与本发明的蛋白质相同的靶标有害生物的昆虫抗性，或可靶向不同的有害生物。靶标昆虫植物有害生物可以通过咀嚼、吸吮或刺穿来摄食。本领域已知干扰RNA可用于昆虫控制。在其他实施例中，干扰RNA可赋予针对非昆虫植物有害生物(如线虫有害生物或病毒有害生物)的抗性。
多于一种的杀有害生物剂在相同转基因植物中的共表达可以通过制造单一的重组载体(在一种所谓的分子堆积(molecular stack)中包含多于一种的杀有害生物剂的编码序列)并对植物进行遗传工程化以便在该转基因植物中含有并表达所有的杀有害生物剂而实现。此类分子叠加还可以通过使用微型染色体进行制备，如在例如美国专利7,235,716中所述的。可替代地，包含对第一杀有害生物剂进行编码的核酸的转基因植物可以用对第二有害生物剂等进行编码的不同的核酸进行再转化。可替代地，可以将植物(亲本1)遗传工程化，用于本发明的基因的表达。可以将第二植物(亲本2)遗传工程化，用于第二杀有害生物剂的表达。通过将亲本1与亲本2杂交，获得了表达被引入至亲本1和亲本2中的所有基因的子代植物。
包含本发明的重组杀昆虫蛋白的转基因植物或种子还可以用杀昆虫剂或杀昆虫种子包衣进行处理，如美国专利号5,849,320和5,876,739(通过引用并入本文)中所述的。在杀昆虫剂或杀昆虫种子包衣以及本发明的转基因植物或种子均对同一靶昆虫(例如鳞翅目靶有害生物)有活性的情况下，该组合(i)在用于进一步增强本发明的组合物对该靶昆虫的活性的方法中，和(ii)在用于通过提供针对该靶昆虫的又另一种作用机制而防止对本发明的组合物产生抗性的方法中是有用的。因此，本发明提供了增强对鳞翅目昆虫群体的控制的方法，该方法包括提供本发明的转基因植物或种子并且向本发明的转基因植物或种子施用杀昆虫剂或杀昆虫种子包衣。
即使在杀昆虫种子包衣对不同昆虫有活性的情况下，该杀昆虫种子包衣对于扩展昆虫控制的范围是有用的，例如通过将对鞘翅目昆虫有活性(在一些实施例中，对鞘翅目和一些鳞翅目昆虫有活性)的杀昆虫种子包衣添加到本发明的转基因种子中，所产生的包被的转基因种子控制鳞翅目和鞘翅目昆虫有害生物两者。
此类杀昆虫剂和/或杀昆虫种子包衣的实例包括但不限于氨基甲酸酯、拟除虫菊酯、有机磷酸酯、friprole、新烟碱、有机氯化物、沙蚕毒素或其组合。在另一个实施例中，杀昆虫剂或杀昆虫种子包衣选自由以下组成的组：克百威、胺甲萘、灭多虫、联苯菊酯、七氟菊酯、氯菊酯、氟氯氰菊酯、λ-三氟氯氰菊酯、氯氰菊酯、溴氰菊酯、毒死蜱、氯氧磷、乐果、灭线磷、马拉硫磷、甲基对硫磷、甲拌磷、特丁磷、叔丁嘧啶磷(tebupirimiphos)、氟虫腈、啶虫脒、吡虫啉、噻虫啉、噻虫嗪、硫丹、杀虫磺、及其组合。包含此类杀昆虫剂和杀昆虫种子包衣的商业产品包括但不限于
Figure BDA0003258410290000511
(克百威)、
Figure BDA0003258410290000512
(灭多虫、灭多威、纳乃得)、
Figure BDA0003258410290000513
(胺甲萘)、
Figure BDA0003258410290000514
(联苯菊酯)、
Figure BDA0003258410290000515
(七氟菊酯)、
Figure BDA0003258410290000516
(氯氰菊酯)、
Figure BDA0003258410290000517
(氯氰菊酯)、Delta
Figure BDA0003258410290000518
(溴氰菊酯)、
Figure BDA0003258410290000519
(λ-氯氟氰菊酯)、
Figure BDA00032584102900005110
(氯菊酯)、
Figure BDA00032584102900005111
(氯菊酯)、
Figure BDA00032584102900005112
(联苯菊酯)、
Figure BDA00032584102900005113
(联苯菊酯)、
Figure BDA00032584102900005114
(七氟菊酯))、
Figure BDA00032584102900005115
(λ氯氟氰菊酯)、
Figure BDA00032584102900005116
(毒死蜱)、
Figure BDA00032584102900005117
(氯氧磷)、
Figure BDA00032584102900005118
(灭克磷)、
Figure BDA00032584102900005119
(甲拌磷)、
Figure BDA00032584102900005120
(甲拌磷、氟氰戊菊酯(flucythinate))、
Figure BDA00032584102900005121
(甲拌磷)、
Figure BDA00032584102900005122
(特丁磷)、
Figure BDA00032584102900005123
(乐果)、异氯磷、
Figure BDA00032584102900005124
(氟虫腈))、
Figure BDA00032584102900005125
(噻虫嗪)、
Figure BDA0003258410290000521
(吡虫啉)、
Figure BDA0003258410290000522
(吡虫啉)、
Figure BDA0003258410290000523
(噻虫胺)和
Figure BDA0003258410290000524
(氟氯氰菊酯、嘧丙磷)。
本发明还涵盖杀昆虫组合物，该杀昆虫组合物包含控昆虫有效量的本发明的重组杀昆虫蛋白。在另外的实施例中，杀昆虫组合物包含合适的农业运载体和本发明的杀昆虫蛋白。本发明的杀昆虫组合物(例如，包含本发明的杀昆虫蛋白和农业上可接受的运载体的组合物)可用于传统农业方法中。农业上可接受的运载体是可用于将包含本发明的多肽的组合物施用至植物或种子的配制品。例如，本发明的组合物可以与水和/或肥料混合，并且可以通过任何手段在出苗前和/或出苗后施用至所希望的场所，如飞机喷雾桶、灌溉设备、直接注射喷雾设备、背负式喷雾桶、家畜浸渍槽、在地面喷雾中使用的农场设备(例如，喷管式喷雾器、手动喷雾器)等。所希望的场所可以是土壤、植物等。本发明的杀昆虫组合物可以在以下时间施用至处于任何生理状态下的种子或植物繁殖体：在种子收获和播种之间的任何时间；或在播种期间或播种后；和/或发芽后。优选种子或植物繁殖体处于足够耐用的状态，使得在处理过程期间不会造成损害或造成最小的损害，包括物理损害或生物损害。配制品可以使用常规的包衣技术以及机器(如流化床技术、滚筒研磨方法、静态转动(rotostatic)种子处理器和转鼓包衣器)施用至种子或植物繁殖体上。
在一些实施例中，本发明涵盖控制昆虫有害生物的方法，该方法包括将有效量的本发明的重组杀昆虫蛋白递送至该昆虫有害生物或其环境。在这些实施例的一些方面，该杀昆虫蛋白通过转基因植物或通过局部施用包含嵌合杀昆虫蛋白的杀昆虫组合物递送。在其他实施例中，重组杀昆虫蛋白包含选自由SEQ ID NO:61-66或SEQ ID NO:115-117组成的组的氨基酸序列。在其他实施例中，杀昆虫组合物包含含有SEQ ID NO:60的杀昆虫Cry1Ab蛋白。在其他实施例中，转基因植物或杀昆虫组合物包含不同于嵌合杀昆虫蛋白的第二杀昆虫剂。在其他实施例中，第二杀昆虫剂是蛋白质或dsRNA。在另外的实施例中，蛋白质选自由以下组成的组：Cry蛋白、营养期杀昆虫蛋白(Vip)、马铃薯糖蛋白、蛋白酶、蛋白酶抑制剂、脲酶、α-淀粉酶抑制剂、成孔蛋白、凝集素、工程化抗体或抗体片段、或几丁质酶、及其组合。
在一些实施例中，本发明还提供了减轻表达本发明的杀昆虫蛋白的转基因玉米植物对暴露于来自转基因玉米植物的花粉的非靶标昆虫的影响的方法，该方法包括向玉米植物中引入本发明的DNA构建体，其中该DNA构建体使得该杀昆虫蛋白在花粉中的表达水平比在叶中更低，从而减轻了转基因玉米花粉对暴露于花粉的非靶标昆虫的影响。
实例
本发明的实施例可以通过参考以下实例而被更好地理解。前述的和以下的本发明的实施例以及各种实施例的描述不是旨在限制权利要求，而是对其具有说明性。因此，应理解的是权利要求不旨在受限于这些实例的具体细节。本领域技术人员应理解的是本发明的其他实施例可以在不偏离本披露内容的精神和范围的情况下进行实践，本披露内容的范围是由所附权利要求限定的。本领域公认的重组DNA和分子克隆技术可以在以下文献中找到，例如J.Sambrook等人,Molecular Cloning:A Laboratory Manual[分子克隆：实验室手册],第3版,Cold Spring Harbor[冷泉港],NY[纽约州]:Cold Spring Harbor LaboratoryPress[冷泉港实验室出版社](2001)；T.J.Silhavy,M.L.Berman,和L.W.Enquist,Experiments with Gene Fusions[基因融合实验],Cold Spring Harbor Laboratory[冷泉港实验室],Cold Spring Harbor[冷泉港],NY[纽约州](1984)和Ausubel,F.M.等人,Current Protocols in Molecular Biology[分子生物学实验手册],New York[纽约],John Wiley and Sons Inc.[约翰威利父子出版公司],(1988),Reiter等人,Methods inArabidopsis Research[拟南芥属研究方法],World Scientific Press[世界科学出版社](1992)，以及Schultz等人,Plant Molecular Biology Manual[植物分子生物学手册],Kluwer Academic Publishers[克鲁沃学术出版社](1998)。
实例1.内源性雄性组织miRNA的鉴定。
花粉特异性和绒毡层特异性miRNA从公共数据库“PmiRExAT”中提取，万维网网址为pmirexat.nabi.res.in/index.html(Gurjar,A.K.S.等人PmiRExAt:Plants miRNAexpression atlas database and web applications[PmiRExAt：植物miRNA表达图谱数据库和网络应用].2016.第2016卷:文章ID baw060；doi:10.1093/database/baw060)。通过提取“File 3:283NR known miR of Maize versus 43datasets Count Matrix Foldchangeand Shannon Entropy[文件3：玉蜀黍的283NR已知miR与43个数据集计数矩阵差异倍数和香农熵]”(可从PmiRExAT下载)中的数据来鉴定花粉特异性miRNA。这个文件含有来自公开可得的研究的玉米miRNA在各种组织中的表达数据，以臂为单位，即3'-臂(3p)或5'-臂(5p)。对于本发明，“花粉特异性”或“绒毡层特异性”miRNA被定义为具有1)相对于其他组织在花粉或绒毡层中表达最高和2)在非花粉或非绒毡层组织中低表达的miRNA。基于这些标准，选择了三种花粉特异性miRNA用于进一步评估，即miRNA159h-3p、miRNA156i-3p和miRNA171i-5p。还从同一数据集中选择了另一个花粉优选的miRNA(miR396b-3p)，因为它在花粉中有高表达(高于miR171i-5p和miR156i-3p)，但在有害生物昆虫捕食的靶组织(例如幼苗、根和花丝)中也有中等水平的表达。还选择了三种绒毡层特异性miRNA，即miR2275a-3p、miR2275b-5p和miR2275b-3p。
实例2.miRNA结合位点的最佳侧翼序列的鉴定。
为了提高miRNA与起始子序列结合的可及性，设计了侧翼序列并将其添加到每个起始子序列的5’和3’末端。侧翼序列被设计成符合以下几点：1)在起始子序列周围提供负的自由能；2)其大小为，在起始子序列的5’末端上有17个碱基且在起始子序列的3’末端上有13个碱基；以及3)低GC含量。因此，本发明的每个miRNA元件包含起始子序列，该起始子序列在5’末端侧翼有17个碱基且在3’末端侧翼有13个碱基。miRNA元件的大小为约51或52个核苷酸。
使用ViennaRNA软件包中的RNAinverse工具(Lorenz等人2011.ViennaRNAPackage 2.0Algorithms for Molecular Biology[ViennaRNA软件包2.0，分子生物学算法],6:26,doi:10.1186/1748-7188-6-26)来为下文所述的每个DNA构建体鉴定出最佳的17bp 5’和13bp 3’侧翼序列。对于使用假定靶基因序列的构建体，靶结合位点周围的17bp5’和13bp 3’序列与21/22个核苷酸的起始子序列一起作为输入提供给RNAinverse。指示RNAinverse保持miRNA起始子序列不变，但修改内源性侧翼序列以产生具有最低自由能的序列。例如，将5’-tcctattcaatgataagAAGAGCACCGTTCACTCCAACtgcaaacatgtgg-3’(SEQ IDNO:122)输入序列改变为5’-tcctattcaaggataagAAGAGCACCGTTCACTCCAACtgcaaacaggtgg-3’(SEQ ID NO:123)输出序列，其中将输入序列中来自5’末端的位置11处的“t”和来自3’末端的位置5处的“t”改变为输出序列中的“g”(加下划线示出)。对于使用与miRNA完全匹配的序列的构建体，从头生成最佳侧翼序列。例如，将5’-nnnnnnnnnnnnnnnnnCAGAGCTCCCTTCACTCCAAAnnnnnnnnnnnnn-3’(SEQ ID N:124)输入序列(其中“n”是任何碱基)改变为
5’-cgctcacgacagcctggCAGAGCTCCCTTCACTCCAAAcattgcgcatctc-3’(SEQ ID NO:125)输出序列。
实例3.具有插入昆虫控制基因的miRNA结合位点的构建体的设计。
制备了多种构建体，这些构建体的不同之处在于：1)插入转基因的成熟miRNA靶标或起始子序列；和2)起始子序列在转基因中的位置，即，起始子序列插入5’非翻译区(5’UTR)、3’非翻译区(3’UTR)或杀昆虫蛋白的编码序列(CDS)内。
此实例中使用的起始子序列与成熟miRNA的21或22个核苷酸的序列(“基序”)完全互补匹配，或者起始子序列与玉米植物内的预测靶基因(“t基因”)相同，即可能包括与成熟miRNA序列的错配。已知与miRNA 3’区有几个错配的起始子序列(在植物中很常见)通常与完全匹配的位点同样有效，有时比完全匹配的位点更有效(Liu Q等人(2014).The PlantCell[植物细胞].26(2):741-53)。一些玉米miRNA的内源性靶基因是已知的并且已经公开(Zhang L等人(2009)PLoS Genet.[公共科学图书馆·遗传学]5(11):e1000716)，但这些研究并不总是区分可由前体miRNA产生的两种可能的成熟miRNA，即3’和5’臂(例如，miR142-3p与miR142-5p)。因此，实例1中所述的每个miRNA的21或22个核苷酸的成熟miRNA均获得自miRBase，并将其用于使用软件scan_for_matches(可在万维网“blog.theseed.org/servers/2010/07/scan-for-matches.html”上获得)、使用Schwab等人2005.Developmental Cell[发育细胞]8:517-527披露的规则来搜索玉蜀黍_B73_REF_5_基因组(MAIZE_B73_REF_5_GENOME)中的潜在靶基因。简而言之，这些规则是：1)只允许在位置2到12(包括端点)之间有一个错配；2)不允许在位置10和11处有错配；3)在位置12之后不允许有超过2个连续错配；以及4)在整个成熟miRNA的长度上总共不超过3个错配(不包括位置1处的错配)。为每个miRNA生成计算预测的靶基因的列表。
scan_for_matches输出的另外的基本分析(例如，成对序列比对)提供了每个预测靶基因(例如，外显子、UTR等)中miRNA的结合位点的位置、miRNA和结合位点序列之间的错配数量、以及相对于miRNA序列的一个或多个错配位置。还收集了来自专有玉蜀黍系的基因图谱表达研究的基因表达数据和来自公共研究的蛋白质表达数据(PMID：27540173)，以鉴定具有针对真正内源性靶基因的预期表达模式谱的假定靶基因(即，与其他组织相比，在花粉中的表达受到抑制)。
最后，将预测靶基因中的结合序列与密切相关但非花粉特异性miRNA或非绒毡层特异性miRNA的序列进行比较，以避免插入有可能被非花粉/非绒毡层特异性miRNA结合的序列，这些序列可能在仍然需要表达以控制有害生物昆虫的玉蜀黍组织(例如叶组织)中无意地下调工程化昆虫控制转基因。表1示出了针对成熟miRNA序列之一(miR159h-3p)生成的数据的实例。
表1.用于选择雄性组织特异性/组织优选的miRNA的样本数据集。
Figure BDA0003258410290000571
基于表1中所示的针对实例1中描述的所有四种花粉miRNA的数据类型，使用cry1Ab编码序列(SEQ ID NO:67)作为杀昆虫转基因制备了十二(12)个构建体。这12个构建体描述于表2中。构建体24366是包含全长cry1Ab-17编码序列且不含任何miRNA元件的对照构建体。Cry1Ab的表达由玉蜀黍泛素启动子和NOS终止子驱动。23466还含有用于玉米转化的PMI可选择标记物表达盒。此外，使用vip3编码序列(SEQ ID NO:118)作为杀昆虫转基因以及miR2275制备了十(10)个构建体。miR2275优先在花药中的绒毡层和前花粉性母细胞中表达。miR2275的存在是为了在转基因玉蜀黍植物中对Vip3A进行花药特异性下调。这10个构建体描述于表3中。构建体23705是包含全长vip3A编码序列且不含任何miRNA元件的对照构建体。Vip3的表达由玉蜀黍泛素启动子和35S终止子驱动。23705还含有用于玉米转化的PMI可选择标记物表达盒。
表2.Cry1Ab miRNA构建体。
Figure BDA0003258410290000581
表3.Vip3 miRNA构建体。
Figure BDA0003258410290000582
实例4.时转化
此实例描述了在瞬时玉蜀黍测定中测试实例3中设计的构建体，以确定具有与本发明所选miRNA相似的结合序列但具有不同组织位置的任何玉蜀黍内源性miRNA是否可以靶向除雄性生殖组织以外的组织(例如叶)中的用于下调的构建体中的工程化转基因。美国专利号8,642,839披露了此玉蜀黍瞬时测定的实例，该专利通过引用并入本文。技术人员将认识到，可以对瞬时测定系统进行改变和修改以实现相同的预期结果。在玉蜀黍叶瞬时测定中测试了实例3中描述的构建体。对于Cry1Ab构建体，将包含全长cry1Ab编码序列且不含miRNA元件的构建体(24366)用作阳性对照。将空载体(EHA101)用作阴性对照。在每个测定中还测试了包含珊瑚荧光蛋白(CFP)的第三对照构建体(18515)。将Cry1Ab蛋白的表达水平针对CFP的表达水平进行归一化。结果示于表4中。
表4.Cry1Ab-miRNA构建体的瞬时玉蜀黍测定的结果。
Figure BDA0003258410290000591
针对所有Cry1Ab-miRNA构建体都检测到Cry1Ab蛋白的表达，尽管其中三个构建体的表达显著低于对照。基于瞬时表达结果，选择以下构建体在稳定的玉蜀黍转化实验中进行测试：24373、24374、24375、24376、24377、24379、24380和24381，以及Cry1Ab对照(24366)。
针对所有Vip3A-miRNA构建体都检测到Vip3A蛋白的表达，尽管其中四个构建体的表达显著低于对照。在稳定转化实验中测试了所有的Vip3A-miR2275构建体。
实例5.玉蜀黍的稳定转化。
此实例描述了在稳定的玉蜀黍转化实验中测试实例4中选择的构建体。
上述每个重组载体包含两个表达盒。第一表达盒包含可操作地连接至编码微小RNA(miRNA)元件的DNA序列的杀昆虫蛋白编码序列，该miRNA元件在玉米植物中是雄性组织特异性的或雄性组织优选的。第一表达盒进一步包含均可操作地连接至编码序列/miRNA元件组合的玉蜀黍泛素启动子和NOS终止子。第二盒包含可操作地连接至编码可选择标记物磷酸甘露糖异构酶(PMI)的pmi编码序列的玉蜀黍Ubi1启动子，该pmi编码序列可操作地连接至玉蜀黍Ubi1终止子。
使用标准分子生物学技术将本发明的重组载体转化到根癌农杆菌(Agrobacterium tumefaciens)中。为了制备用于转化的农杆菌，将细胞在28℃和220rpm下在液体YPC培养基中培养过夜。
基本上如Negrotto等人,2000,Plant Cell Reports[植物细胞报告]19:798-803中所述进行未成熟玉蜀黍胚的农杆菌转化。对于这个实例，所有的培养基组分基本上如Negrotto等人(如上)所述。然而，本领域内已知的多种培养基组分可以被替代。
简言之，使包含二元载体植物转化载体的农杆菌菌株LBA4404(pSB1)在28℃在YEP(酵母提取物(5g/L)、蛋白胨(10g/L)、NaCl(5g/L)、15g/l琼脂，pH 6.8)固体培养基上生长2-4天。使大约0.8X109个农杆菌悬浮于补充有100μM As的LS-inf培养基中(Negrotto等人，同上)。在此培养基中对细菌预诱导大约30-60分钟。
将来自合适玉米基因型的未成熟胚从8-12天大的穗中切除到液体LS-inf+100μMAs中。将胚胎用新鲜的感染培养基冲洗一次。然后添加农杆菌溶液，并且将这些胚涡旋30秒并使其与细菌一起沉降约5分钟。然后将这些胚盾片向上地转移到LSA培养基中，并且在黑暗中培养两至三天。随后，将20与25个之间的胚/培养皿转移到补充有头孢噻肟(250mg/l)和硝酸银(1.6mg/l)的LSDc培养基中，并且在28℃在黑暗中培养10天。
将产生胚性愈伤组织的未成熟的胚转移到LSD1M0.5S培养基中。在此培养基上选择培养物约6周，在约3周时进行继代培养步骤。将存活着的愈伤组织转移到补充有甘露糖的Reg1培养基中。然后在光照中(16小时光照/8小时黑暗方案)培养之后，将绿色组织转移至没有生长调节剂的Reg2培养基中并孵育约1至2周。将小植株转移到含有Reg3培养基的Magenta GA-7盒(伊利诺斯州芝加哥的马真塔公司(Magenta Corp,Chicago Ill.))中，并且使它们在光照中生长。
在转化、选择和再生之后，使用
Figure BDA0003258410290000611
分析来测定T0植物中是否存在pmi基因和cry1Ab或vip3A编码序列。还测试了植物中是否存在载体骨架。将对载体骨架呈阴性并且包含来自重组载体的转基因的一个拷贝的转基因玉蜀黍植物转移到温室中，并测试其对鳞翅目昆虫有害生物(例如，欧洲玉米螟和秋夜蛾)的杀昆虫活性。
实例6.Cry1Ab表达的表达水平和模式。
此实例描述了T0植物分析，以确定用实例3中描述的Cry1Ab-miRNA构建体稳定转化的T0转基因玉米植物中Cry1Ab蛋白的表达水平。
玉米的生长阶段分为营养阶段(V)和生殖阶段(R)。V阶段的细分在数字上被指定为V1、V2、V3等，直到V(n)，其中(n)代表抽穗前的最后一个叶阶段(VT)。生殖阶段在数字上被指定为R1＝抽丝到R6＝成熟。
使实例5中选择的转基因玉米植物在温室中在控制条件下生长到R1阶段。在R1阶段，从针对每个构建体的多个T0植物的叶、花粉和花丝中取样，并分别使用qRT-PCR和ELISA测量昆虫对照转基因和蛋白质的表达水平。此外，对叶和花丝组织进行了昆虫生物测定，以确保由工程化转基因编码的杀昆虫蛋白保留对鳞翅目昆虫有害生物的毒性。ELISA测定的结果如表5和图1所示。
结果表明，包含重组DNA构建体的玉米植物在雄性生殖组织中比在叶组织中具有较低的杀昆虫蛋白表达，这些重组DNA构建体包含可操作地连接至编码微小RNA(miRNA)元件的DNA序列的杀昆虫蛋白编码序列，该miRNA元件是雄性组织特异性的或雄性组织优选的。构建体24374、24375、24376、24377和24379在花粉中的Cry1Ab水平明显低于叶。除24379外，所有这些构建体都在Cry1Ab编码序列中具有miRNA元件。令人惊讶的是，除24379外，在5’UTR中包含miRNA元件的构建体在花粉中的Cry1Ab水平明显高于叶。
表5.Cry1Ab蛋白在T0转基因玉米植物中的表达水平。
Figure BDA0003258410290000621
Figure BDA0003258410290000631
使用组织切除生物测定法，分别测试了来自R1阶段的多个T0植物的叶和花丝组织对欧洲玉米螟(ECB；欧洲玉米螟(Ostrinia nubilalis))和玉米穗蛾(CEW；谷实夜蛾(Helicoverpa zea))的杀昆虫活性。简而言之，从单个植物上切除植物组织(叶和/或花丝)并放在可密封的容器中。用鳞翅目靶有害生物的新生幼虫侵染每个组织样品，然后在室温孵育约5天。
表6所示的生物测定结果表明，本发明的Cry1Ab-miRNA构建体在叶和花丝中的表达水平足够高以控制预期的靶有害生物，但24377构建体除外。尽管在叶生物测定中，测试的包含24377构建体的100％T0植物对ECB产生了100％的死亡率，但在花丝生物测定中，没有任何T0植物对CEW产生100％的死亡率。
表6.来自包含DNA构建体的T0植物的组织的昆虫生物测定结果。
Figure BDA0003258410290000632
实例7.Vip3A表达的表达水平和模式。
此实例描述了T0植物分析，以确定用实例3中描述的Vip3A-miRNA构建体稳定转化的T0转基因玉米植物中Vip3A蛋白的表达水平。
使实例5中选择的转基因玉米植物在温室中在控制条件下生长到R1阶段。在R1阶段，从针对每个构建体的多个T0事件的叶、花粉和花丝中取样，并分别使用qRT-PCR和ELISA测量昆虫对照转基因和蛋白质的表达水平。此外，对叶(秋夜蛾；FAW)和花丝组织(玉米穗蛾；CEW)进行了昆虫生物测定，以确保由工程化转基因编码的Vip3蛋白保留对鳞翅目昆虫有害生物的毒性。ELISA测定和生物测定的结果示于表7中。叶和花丝的生物测定结果显示为“++”、“+”或“-”，表示具有杀昆虫的叶和/或花丝的T0事件的百分比，其中“++”＝≥50％的事件，“+”＝<50％的事件，“-”＝0％的事件。
表7.T0事件的Vip3A表达水平和生物测定结果。
Figure BDA0003258410290000641
结果表明，包含重组DNA构建体的转基因玉米事件(植物)在雄性生殖组织中比在叶组织中具有较低的Vip3表达，这些重组DNA构建体包含可操作地连接至编码微小RNA(miRNA)元件的DNA序列的Vip3杀昆虫蛋白编码序列，该miRNA元件是雄性组织特异性的或雄性组织优选的。与阳性对照相比，构建体23712、23708、23713、23714、23718、23716和23717在花药(miR2275 RNA具有活性的组织)中的Vip3水平显著低于叶。一些构建体不仅在花药中具有较低的Vip3水平，而且在miR2275不具有活性的组织中也具有较低的Vip3蛋白水平。如果其他组织中较低的Vip3水平不低于杀昆虫水平，则此类构建体仍然有用。这是构建体23708、23714和23715的情况，其中叶和花丝中的Vip3水平低于对照，但来自包含这些构建体的植物的叶组织和花丝组织仍然对靶有害生物具有杀昆虫作用。与阳性对照相比，构建体23713在叶和花丝组织中具有较低的Vip3蛋白水平，但只有来自包含该构建体的转基因事件的叶组织对靶有害生物具有杀昆虫作用。所有在Vip3编码序列中具有miR2275元件的构建体均产生了在所有分析的组织中具有较低Vip3水平的植物。然而，包含构建体中的两个(23718和23717)的转基因事件具有对靶有害生物有杀昆虫作用的叶。在花药中Vip3蛋白减少最高、同时在叶和花丝中保持杀昆虫活性的构建体是23708、23714和23712。所有这三个构建体都包含miR2275的3p臂，具有基序起始子序列类型，并且具有位于Vip3基因的3’UTR中的miR2275起始子序列。
如上所述地测试和分析了来自包含构建体23705、23712、23708、23713和23717的转基因T0事件的下一代T1植物。将包含磷酸甘露糖异构酶(PMI)基因并且不包含miR2275起始子序列的可选择标记物构建体用作阴性对照。T1植物分析的结果示于表8中。
表8.T1植物的Vip3表达水平和生物测定结果。
Figure BDA0003258410290000651
与T0事件一样，由于存在miR2275起始子序列和miR2275微小RNA的内源性活性，与阳性对照相比，包含构建体23708和23712的T1植物在花药中具有显著较低的Vip3蛋白水平。不包含微小RNA起始子序列的PMI表达盒在所有的构建处理和组织中表达同等水平的PMI蛋白。这些结果表明miR2275a和miR2275b均可以识别它们各自在植物中的起始子序列，以下调杀昆虫蛋白(像Vip3)在雄性生殖组织或玉米中的表达。
实例8.在植物细胞中原位编辑基因组以生成经修饰的嵌合蛋白。
此实例说明了使用原位植物细胞基因组的基因组编辑来掺入突变，从而通过将miRNA元件掺入野生型Cry1Ab或Vip3杀昆虫蛋白的编码序列或掺入已修饰的Cry1Ab或Vip3蛋白的编码序列中来制备本文所述的DNA构建体(包括但不限于实例3中所述的DNA构建体)。
定向基因组修饰(也称为基因组编辑)可用于在特定DNA序列中引入突变。这些基因组编辑技术，其包括锌指核酸酶(ZNF)、转录激活子样效应子核酸酶(TALEN)、大范围核酸酶和成簇规律间隔短回文重复(CRISPR)，已成功施用至包括作物植物在内的50多种不同生物。参见例如，Belhaj,K.等人,Plant Methods[植物方法]9,39(2013)；Jiang,W.等人,Nucleic Acids Res[核酸研究]41,e188(2013))。用于基因组编辑的CRISPR/Cas系统基于Cas9核酸酶和指定靶多核苷酸序列的工程化单指导RNA(sgRNA)的瞬时表达。
Cas9是一种大的单体DNA核酸酶，其借助两个20个核苷酸(nt)的非编码RNA的复合物被引导至DNA靶序列：CRIPSR RNA(crRNA)和反式激活crRNA(tracrRNA)，其功能上可作为单合成RNA嵌合体获得。Cas9蛋白含有两个与RuvC和HNH核酸酶同源的核酸酶结构域。HNH核酸酶结构域切割互补DNA链，而RuvC样结构域切割非互补链，因此，在靶DNA中引入钝的切口。
当Cas9和sgRNA在活的玉蜀黍细胞中瞬时表达时，在转基因玉蜀黍细胞中产生特异性靶DNA中的双链断裂(DSB)。通过非同源末端连接和同源定向DNA修复途径引入断裂位点处的突变。
通过使用表达Cas9核酸酶的重组质粒和针对转基因玉蜀黍中的cry1Ab或vip3或者经修饰的cry1Ab或vip3序列进行玉蜀黍密码子优化的sgRNA靶标，将特定突变引入cry1Ab编码序列(例如SEQ ID NO:67)、或vip3编码序列(例如SEQ ID NO:118)、或经修饰的cry1Ab和/或vip3编码序列中。该方法的实施是通过根癌农杆菌的农杆菌浸润方法，该根癌农杆菌携带含有指定的目的靶序列的二元质粒。sgRNA与靶杀昆虫蛋白编码序列结合后，Cas9核酸酶对编码序列进行特异性切割，并在DNA修复过程中引入所希望的一个或多个突变，例如将miRNA元件引入cry1Ab编码序列中。因此，此时突变的cry1Ab杀昆虫蛋白编码序列将编码Cry1Ab-miRNA杀昆虫蛋白。
通过PCR和测序筛选包含基因组编辑的cry1Ab-miRNA杀昆虫蛋白编码序列的植物细胞。诱导具有基因组编辑的cry1Ab-miRNA杀昆虫蛋白编码序列的愈伤组织，以再生植物来对所表达的杀昆虫蛋白对鳞翅目有害生物昆虫的杀昆虫活性进行表型评估，并且测试与叶组织相比在花粉中的表达的降低。
应当理解的是，本文描述的实例和实施例仅是出于说明性的目的，并且根据其说明书的不同修改或变化将提示本领域的技术人员并且将会包含在本申请的精神和范围内以及所附权利要求的范围内。
在本说明书中提到的所有公开物和专利申请均指示本发明所属领域中的技术人员的技术水平。所有公开物和专利申请均通过引用并入本文，其程度如同每个单独的公开物或专利申请被明确地并单独地指示通过引用而并入。
序列表
<110> Syngenta Crop Protection LLC
GREEN, Julie
KELLIHER, Tim
<120> 转基因表达的调节
<130> 81814-US-L-ORG-NAT-1
<160> 125
<170> PatentIn version 3.5
<210> 1
<211> 63
<212> RNA
<213> 人工序列
<220>
<223> 24373 miR159h-3p靶插入序列
<400> 1
acuagcaucc uuuucauuga uaagaagagc accguucacu ccaacugcaa acuuguggcu 60
agu 63
<210> 2
<211> 63
<212> RNA
<213> 人工序列
<220>
<223> 24374 miR156i-3p靶插入序列
<400> 2
acuagcacau ccaacacgca cgagaugaca gauagagcag ugagcagacc aucucuuacu 60
agu 63
<210> 3
<211> 60
<212> RNA
<213> 人工序列
<220>
<223> 24375 miR171i-5p靶插入序列
<400> 3
acuagcaucu cuguugccuu uuugauugaa ccgugccaac agcuuguugg auuuacuagu 60
<210> 4
<211> 63
<212> RNA
<213> 人工序列
<220>
<223> 24376 miR396b-3p靶插入序列
<400> 4
acuagcgacg accccaaagg acagguuucc cacagcuuua uugaacaauu guuugcaucu 60
agu 63
<210> 5
<211> 60
<212> RNA
<213> 人工序列
<220>
<223> 24377 miR396b-3p靶插入序列
<400> 5
acuaguccuu ucucucucuu ucuuucucuc ugcuuuauug aacuucucaa aguuacuagu 60
<210> 6
<211> 57
<212> RNA
<213> 人工序列
<220>
<223> 24379 miR159h-3p靶插入序列
<400> 6
cuagcauccu uuucauugau aagaagagca ccguucacuc caacugcaaa cuugugg 57
<210> 7
<211> 56
<212> RNA
<213> 人工序列
<220>
<223> 24380 miR156i-3p靶插入序列
<400> 7
cuagacaucc aacacgcacg agaugacaga uagagcagug agcugaccau cucuua 56
<210> 8
<211> 54
<212> RNA
<213> 人工序列
<220>
<223> 24381 miR171i-5p靶插入序列
<400> 8
cuagcaucuc uguugccuuu uugauugaac cgugccaaca gcuuguugga uuua 54
<210> 9
<211> 54
<212> RNA
<213> 人工序列
<220>
<223> 24383 miR396b-3p靶插入序列
<400> 9
cuaguccuuu cucucucuuu cuuucucucu gcuuuauuga acuucucaaa guua 54
<210> 10
<211> 63
<212> DNA
<213> 人工序列
<220>
<223> miR159h-3p靶插入序列
<400> 10
actagcatcc ttttcattga taagaagagc accgttcact ccaactgcaa acttgtggct 60
agt 63
<210> 11
<211> 63
<212> DNA
<213> 人工序列
<220>
<223> 24374 miR156i-3p靶插入序列
<400> 11
actagcacat ccaacacgca cgagatgaca gatagagcag tgagcagacc atctcttact 60
agt 63
<210> 12
<211> 60
<212> DNA
<213> 人工序列
<220>
<223> 24375 miR171i-5p靶插入序列
<400> 12
actagcatct ctgttgcctt tttgattgaa ccgtgccaac agcttgttgg atttactagt 60
<210> 13
<211> 63
<212> DNA
<213> 人工序列
<220>
<223> 24376 miR396b-3p靶插入序列
<400> 13
actagcgacg accccaaagg acaggtttcc cacagcttta ttgaacaatt gtttgcatct 60
agt 63
<210> 14
<211> 60
<212> DNA
<213> 人工序列
<220>
<223> 24377 miR396b-3p靶插入序列
<400> 14
actagtcctt tctctctctt tctttctctc tgctttattg aacttctcaa agttactagt 60
<210> 15
<211> 57
<212> DNA
<213> 人工序列
<220>
<223> 24379 miR159h-3p靶插入序列
<400> 15
ctagcatcct tttcattgat aagaagagca ccgttcactc caactgcaaa cttgtgg 57
<210> 16
<211> 56
<212> DNA
<213> 人工序列
<220>
<223> 24380 miR156i-3p靶插入序列
<400> 16
ctagacatcc aacacgcacg agatgacaga tagagcagtg agctgaccat ctctta 56
<210> 17
<211> 54
<212> DNA
<213> 人工序列
<220>
<223> 24381 miR171i-5p靶插入序列
<400> 17
ctagcatctc tgttgccttt ttgattgaac cgtgccaaca gcttgttgga ttta 54
<210> 18
<211> 54
<212> DNA
<213> 人工序列
<220>
<223> 24383 miR396b-3p靶插入序列
<400> 18
ctagtccttt ctctctcttt ctttctctct gctttattga acttctcaaa gtta 54
<210> 19
<211> 5098
<212> DNA
<213> 人工序列
<220>
<223> 24366 cry1Ab表达盒
<400> 19
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 60
agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 120
tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 180
tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 240
gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 300
ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 360
gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 420
agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 480
taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 540
aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 600
cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 660
cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 720
acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 780
ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 840
gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 900
ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 960
cccgtcggca cctccgcttc ctaggtacgc cgctcgtcct cccccccccc ccctctctac 1020
cttctctaga tcggcgttcc ggtccatagt tagggcccgg tagttctact tctgttcatg 1080
tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga 1140
cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg 1200
ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca 1260
tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt 1320
catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt 1380
ctagatcgga gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt 1440
atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc 1500
taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt 1560
tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt 1620
agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca 1680
tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca 1740
tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg 1800
ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tattttgatc 1860
ttgatatact tggatgatgg catatccagc agctatatgt ggattttttt agccctgcct 1920
tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg 1980
tgttacttct gcaggatcct ctaagtagca gccatggaca acaaccccaa catcaacgag 2040
tgcatcccct acaactgcct gagcaacccc gaggtggagg tgctgggcgg cgagcgcatc 2100
gagaccggct acacccccat cgacatcagc ctgagcctga cccagttcct gctgagcgag 2160
ttcgtgcccg gcgccggctt cgtgctgggc ctggtggaca tcatctgggg catcttcggc 2220
cccagccagt gggacgcctt cctggtgcag atcgagcagt tgataaacca acgcatagag 2280
gaattcgccc gcaaccaggc catcagccgc ctggagggcc tgagcaacct gtaccaaatc 2340
tacgccgaga gcttccgcga gtgggaggcc gaccccacca accccgccct gcgcgaggag 2400
atgcgcatcc agttcaacga catgaacagc gccctgacca ccgccatccc cctgttcgcc 2460
gtgcagaact accaggtgcc cctgctgagc gtgtacgtgc aggccgccaa cctgcacctg 2520
agcgtgctgc gcgacgtcag cgtgttcggc cagcgctggg gcttcgacgc cgccaccatc 2580
aacagccgct acaacgacct gacccgcctg atcggcaact acaccgacca cgccgtgcgc 2640
tggtacaaca ccggcctgga gcgcgtgtgg gggcccgaca gccgcgactg gatcaggtac 2700
aaccagttcc gccgcgagct gaccctgacc gtgctggaca tcgtgagcct gttccccaac 2760
tacgacagcc gcacctaccc catccgcacc gtgagccagc tgacccgcga gatttacacc 2820
aaccccgtgc tggagaactt cgacggcagc ttccgcggca gcgcccaggg catcgagggc 2880
agcatccgca gcccccacct gatggacatc ctgaacagca tcaccatcta caccgacgcc 2940
caccgcggcg agtactactg gagcggccac cagatcatgg ccagccccgt cggcttcagc 3000
ggccccgagt tcaccttccc cctgtacggc acgatgggca acgctgcacc tcagcagcgc 3060
atcgtggcac agctgggcca gggagtgtac cgcaccctga gcagcaccct gtaccgtcga 3120
cctttcaaca tcggcatcaa caaccagcag ctgagcgtgc tggacggcac cgagttcgcc 3180
tacggcacca gcagcaacct gcccagcgcc gtgtaccgca agagcggcac cgtggacagc 3240
ctggacgaga tcccccctca gaacaacaac gtgccacctc gacagggctt cagccaccgt 3300
ctgagccacg tgagcatgtt ccgcagtggc ttcagcaaca gcagcgtgag catcatccgt 3360
gcacctatgt tcagctggat tcaccgcagt gccgagttca acaacatcat ccccagcagc 3420
cagatcaccc agatccccct gaccaagagc accaacctgg gcagcggcac cagcgtggtg 3480
aagggccccg gcttcaccgg cggcgacatc ctgcgccgca ccagccccgg ccagatcagc 3540
accctgcgcg tgaacatcac cgcccccctg agccagcgct accgcgtccg catccgctac 3600
gccagcacca ccaacctgca gttccacacc agcatcgacg gccgccccat caaccagggc 3660
aacttcagcg ccaccatgag cagcggcagc aacctgcaga gcggcagctt ccgcaccgtg 3720
ggcttcacca cccccttcaa cttcagcaac ggcagcagcg tgttcaccct gagcgcccac 3780
gtgttcaaca gcggcaacga ggtgtacatc gaccgcatcg agttcgtgcc cgccgaggtg 3840
accttcgagg ccgagtacga cctggagagg gctcagaagg ccgtgaacga gctgttcacc 3900
agcagcaacc agatcggcct gaagaccgac gtgaccgact accacatcga ccaggtgagc 3960
aacctggtgg agtgcttaag cgacgagttc tgcctggacg agaagaagga gctgagcgag 4020
aaggtgaagc acgccaagcg cctgagcgac gagcgcaacc tgctgcagga ccccaacttc 4080
cgcggcatca accgccagct ggaccgcggc tggcgaggca gcaccgatat caccatccag 4140
ggcggcgacg acgtgttcaa ggagaactac gtgaccctgc tgggcacctt cgacgagtgc 4200
taccccacct acctgtacca gaagatcgac gagagcaagc tgaaggccta cacccgctac 4260
cagctgcgcg gctacatcga ggacagccag gacctggaaa tctacctgat ccgctacaac 4320
gcgaagcacg agaccgtgaa cgtgcccggc accggcagcc tgtggcccct gagcgccccc 4380
agccccatcg gcaagtgcac tagtcaccac agccaccact tcagcctgga catcgacgtg 4440
ggctgcaccg acctgaacga ggacctgggc gtgtgggtga tcttcaagat caagacccag 4500
gacggccacg cccgcctggg caatctagag ttcctggagg agaagcccct ggtgggcgag 4560
gccctggccc gcgtgaagcg tgctgagaag aagtggcgcg acaagcgcga gaagctggag 4620
tgggagacca acatcgtgta caaggaggcc aaggagagcg tggacgccct gttcgtgaac 4680
agccagtacg accgcctgca ggccgacacc aacatcgcca tgatccacgc cgccgacaag 4740
cgcgtgcaca gcattcgcga ggcctacctg cccgagctga gcgtgatccc cggtgtgaac 4800
gccgccatct tcgaggaact cgagggccgc atctaggagc tctatgatcg ttcaaacatt 4860
tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa 4920
tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg 4980
agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa 5040
atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatc 5098
<210> 20
<211> 4171
<212> DNA
<213> 人工序列
<220>
<223> 24373 cry1Ab表达盒
<400> 20
gtacgccgct cgtcctcccc ccccccccct ctctaccttc tctagatcgg cgttccggtc 60
catagttagg gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt 120
tagatccgtg ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat 180
tgctaacttg ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga 240
cgggatcgat ttcatgattt tttttgtttc gttgcatagg gtttggtttg cccttttcct 300
ttatttcaat atatgccgtg cacttgtttg tcgggtcatc ttttcatgct tttttttgtc 360
ttggttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag aattctgttt 420
caaactacct ggtggattta ttaattttgg atctgtatgt gtgtgccata catattcata 480
gttacgaatt gaagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc 540
gggttttact gatgcatata cagagatgct ttttgttcgc ttggttgtga tgatgtggtg 600
tggttgggcg gtcgttcatt cgttctagat cggagtagaa tactgtttca aactacctgg 660
tgtatttatt aattttggaa ctgtatgtgt gtgtcataca tcttcatagt tacgagttta 720
agatggatgg aaatatcgat ctaggatagg tatacatgtt gatgtgggtt ttactgatgc 780
atatacatga tggcatatgc agcatctatt catatgctct aaccttgagt acctatctat 840
tataataaac aagtatgttt tataattatt ttgatcttga tatacttgga tgatggcata 900
tccagcagct atatgtggat ttttttagcc ctgccttcat acgctattta tttgcttggt 960
actgtttctt ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag gatcctctaa 1020
gtagcagcca tggacaacaa ccccaacatc aacgagtgca tcccctacaa ctgcctgagc 1080
aaccccgagg tggaggtgct gggcggcgag cgcatcgaga ccggctacac ccccatcgac 1140
atcagcctga gcctgaccca gttcctgctg agcgagttcg tgcccggcgc cggcttcgtg 1200
ctgggcctgg tggacatcat ctggggcatc ttcggcccca gccagtggga cgccttcctg 1260
gtgcagatcg agcagttgat aaaccaacgc atagaggaat tcgcccgcaa ccaggccatc 1320
agccgcctgg agggcctgag caacctgtac caaatctacg ccgagagctt ccgcgagtgg 1380
gaggccgacc ccaccaaccc cgccctgcgc gaggagatgc gcatccagtt caacgacatg 1440
aacagcgccc tgaccaccgc catccccctg ttcgccgtgc agaactacca ggtgcccctg 1500
ctgagcgtgt acgtgcaggc cgccaacctg cacctgagcg tgctgcgcga cgtcagcgtg 1560
ttcggccagc gctggggctt cgacgccgcc accatcaaca gccgctacaa cgacctgacc 1620
cgcctgatcg gcaactacac cgaccacgcc gtgcgctggt acaacaccgg cctggagcgc 1680
gtgtgggggc ccgacagccg cgactggatc aggtacaacc agttccgccg cgagctgacc 1740
ctgaccgtgc tggacatcgt gagcctgttc cccaactacg acagccgcac ctaccccatc 1800
cgcaccgtga gccagctgac ccgcgagatt tacaccaacc ccgtgctgga gaacttcgac 1860
ggcagcttcc gcggcagcgc ccagggcatc gagggcagca tccgcagccc ccacctgatg 1920
gacatcctga acagcatcac catctacacc gacgcccacc gcggcgagta ctactggagc 1980
ggccaccaga tcatggccag ccccgtcggc ttcagcggcc ccgagttcac cttccccctg 2040
tacggcacga tgggcaacgc tgcacctcag cagcgcatcg tggcacagct gggccaggga 2100
gtgtaccgca ccctgagcag caccctgtac cgtcgacctt tcaacatcgg catcaacaac 2160
cagcagctga gcgtgctgga cggcaccgag ttcgcctacg gcaccagcag caacctgccc 2220
agcgccgtgt accgcaagag cggcaccgtg gacagcctgg acgagatccc ccctcagaac 2280
aacaacgtgc cacctcgaca gggcttcagc caccgtctga gccacgtgag catgttccgc 2340
agtggcttca gcaacagcag cgtgagcatc atccgtgcac ctatgttcag ctggattcac 2400
cgcagtgccg agttcaacaa catcatcccc agcagccaga tcacccagat ccccctgacc 2460
aagagcacca acctgggcag cggcaccagc gtggtgaagg gccccggctt caccggcggc 2520
gacatcctgc gccgcaccag ccccggccag atcagcaccc tgcgcgtgaa catcaccgcc 2580
cccctgagcc agcgctaccg cgtccgcatc cgctacgcca gcaccaccaa cctgcagttc 2640
cacaccagca tcgacggccg ccccatcaac cagggcaact tcagcgccac catgagcagc 2700
ggcagcaacc tgcagagcgg cagcttccgc accgtgggct tcaccacccc cttcaacttc 2760
agcaacggca gcagcgtgtt caccctgagc gcccacgtgt tcaacagcgg caacgaggtg 2820
tacatcgacc gcatcgagtt cgtgcccgcc gaggtgacct tcgaggccga gtacgacctg 2880
gagagggctc agaaggccgt gaacgagctg ttcaccagca gcaaccagat cggcctgaag 2940
accgacgtga ccgactacca catcgaccag gtgagcaacc tggtggagtg cttaagcgac 3000
gagttctgcc tggacgagaa gaaggagctg agcgagaagg tgaagcacgc caagcgcctg 3060
agcgacgagc gcaacctgct gcaggacccc aacttccgcg gcatcaaccg ccagctggac 3120
cgcggctggc gaggcagcac cgatatcacc atccagggcg gcgacgacgt gttcaaggag 3180
aactacgtga ccctgctggg caccttcgac gagtgctacc ccacctacct gtaccagaag 3240
atcgacgaga gcaagctgaa ggcctacacc cgctaccagc tgcgcggcta catcgaggac 3300
agccaggacc tggaaatcta cctgatccgc tacaacgcga agcacgagac cgtgaacgtg 3360
cccggcaccg gcagcctgtg gcccctgagc gcccccagcc ccatcggcaa gtgcactagc 3420
atccttttca ttgataagaa gagcaccgtt cactccaact gcaaacttgt ggctagtcac 3480
cacagccacc acttcagcct ggacatcgac gtgggctgca ccgacctgaa cgaggacctg 3540
ggcgtgtggg tgatcttcaa gatcaagacc caggacggcc acgcccgcct gggcaatcta 3600
gagttcctgg aggagaagcc cctggtgggc gaggccctgg cccgcgtgaa gcgtgctgag 3660
aagaagtggc gcgacaagcg cgagaagctg gagtgggaga ccaacatcgt gtacaaggag 3720
gccaaggaga gcgtggacgc cctgttcgtg aacagccagt acgaccgcct gcaggccgac 3780
accaacatcg ccatgatcca cgccgccgac aagcgcgtgc acagcattcg cgaggcctac 3840
ctgcccgagc tgagcgtgat ccccggtgtg aacgccgcca tcttcgagga actcgagggc 3900
cgcatctagg agctctatga tcgttcaaac atttggcaat aaagtttctt aagattgaat 3960
cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta 4020
ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg 4080
caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta 4140
tcgcgcgcgg tgtcatctat gttactagat c 4171
<210> 21
<211> 5155
<212> DNA
<213> 人工序列
<220>
<223> 24374 cry1Ab表达盒
<400> 21
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 60
agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 120
tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 180
tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 240
gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 300
ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 360
gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 420
agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 480
taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 540
aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 600
cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 660
cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 720
acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 780
ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 840
gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 900
ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 960
cccgtcggca cctccgcttc ctaggtacgc cgctcgtcct cccccccccc ccctctctac 1020
cttctctaga tcggcgttcc ggtccatagt tagggcccgg tagttctact tctgttcatg 1080
tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga 1140
cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg 1200
ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca 1260
tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt 1320
catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt 1380
ctagatcgga gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt 1440
atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc 1500
taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt 1560
tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt 1620
agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca 1680
tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca 1740
tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg 1800
ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tattttgatc 1860
ttgatatact tggatgatgg catatccagc agctatatgt ggattttttt agccctgcct 1920
tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg 1980
tgttacttct gcaggatcct ctaagtagca gccatggaca acaaccccaa catcaacgag 2040
tgcatcccct acaactgcct gagcaacccc gaggtggagg tgctgggcgg cgagcgcatc 2100
gagaccggct acacccccat cgacatcagc ctgagcctga cccagttcct gctgagcgag 2160
ttcgtgcccg gcgccggctt cgtgctgggc ctggtggaca tcatctgggg catcttcggc 2220
cccagccagt gggacgcctt cctggtgcag atcgagcagt tgataaacca acgcatagag 2280
gaattcgccc gcaaccaggc catcagccgc ctggagggcc tgagcaacct gtaccaaatc 2340
tacgccgaga gcttccgcga gtgggaggcc gaccccacca accccgccct gcgcgaggag 2400
atgcgcatcc agttcaacga catgaacagc gccctgacca ccgccatccc cctgttcgcc 2460
gtgcagaact accaggtgcc cctgctgagc gtgtacgtgc aggccgccaa cctgcacctg 2520
agcgtgctgc gcgacgtcag cgtgttcggc cagcgctggg gcttcgacgc cgccaccatc 2580
aacagccgct acaacgacct gacccgcctg atcggcaact acaccgacca cgccgtgcgc 2640
tggtacaaca ccggcctgga gcgcgtgtgg gggcccgaca gccgcgactg gatcaggtac 2700
aaccagttcc gccgcgagct gaccctgacc gtgctggaca tcgtgagcct gttccccaac 2760
tacgacagcc gcacctaccc catccgcacc gtgagccagc tgacccgcga gatttacacc 2820
aaccccgtgc tggagaactt cgacggcagc ttccgcggca gcgcccaggg catcgagggc 2880
agcatccgca gcccccacct gatggacatc ctgaacagca tcaccatcta caccgacgcc 2940
caccgcggcg agtactactg gagcggccac cagatcatgg ccagccccgt cggcttcagc 3000
ggccccgagt tcaccttccc cctgtacggc acgatgggca acgctgcacc tcagcagcgc 3060
atcgtggcac agctgggcca gggagtgtac cgcaccctga gcagcaccct gtaccgtcga 3120
cctttcaaca tcggcatcaa caaccagcag ctgagcgtgc tggacggcac cgagttcgcc 3180
tacggcacca gcagcaacct gcccagcgcc gtgtaccgca agagcggcac cgtggacagc 3240
ctggacgaga tcccccctca gaacaacaac gtgccacctc gacagggctt cagccaccgt 3300
ctgagccacg tgagcatgtt ccgcagtggc ttcagcaaca gcagcgtgag catcatccgt 3360
gcacctatgt tcagctggat tcaccgcagt gccgagttca acaacatcat ccccagcagc 3420
cagatcaccc agatccccct gaccaagagc accaacctgg gcagcggcac cagcgtggtg 3480
aagggccccg gcttcaccgg cggcgacatc ctgcgccgca ccagccccgg ccagatcagc 3540
accctgcgcg tgaacatcac cgcccccctg agccagcgct accgcgtccg catccgctac 3600
gccagcacca ccaacctgca gttccacacc agcatcgacg gccgccccat caaccagggc 3660
aacttcagcg ccaccatgag cagcggcagc aacctgcaga gcggcagctt ccgcaccgtg 3720
ggcttcacca cccccttcaa cttcagcaac ggcagcagcg tgttcaccct gagcgcccac 3780
gtgttcaaca gcggcaacga ggtgtacatc gaccgcatcg agttcgtgcc cgccgaggtg 3840
accttcgagg ccgagtacga cctggagagg gctcagaagg ccgtgaacga gctgttcacc 3900
agcagcaacc agatcggcct gaagaccgac gtgaccgact accacatcga ccaggtgagc 3960
aacctggtgg agtgcttaag cgacgagttc tgcctggacg agaagaagga gctgagcgag 4020
aaggtgaagc acgccaagcg cctgagcgac gagcgcaacc tgctgcagga ccccaacttc 4080
cgcggcatca accgccagct ggaccgcggc tggcgaggca gcaccgatat caccatccag 4140
ggcggcgacg acgtgttcaa ggagaactac gtgaccctgc tgggcacctt cgacgagtgc 4200
taccccacct acctgtacca gaagatcgac gagagcaagc tgaaggccta cacccgctac 4260
cagctgcgcg gctacatcga ggacagccag gacctggaaa tctacctgat ccgctacaac 4320
gcgaagcacg agaccgtgaa cgtgcccggc accggcagcc tgtggcccct gagcgccccc 4380
agccccatcg gcaagtgcac tagcacatcc aacacgcacg agatgacaga tagagcagtg 4440
agcagaccat ctcttactag tcaccacagc caccacttca gcctggacat cgacgtgggc 4500
tgcaccgacc tgaacgagga cctgggcgtg tgggtgatct tcaagatcaa gacccaggac 4560
ggccacgccc gcctgggcaa tctagagttc ctggaggaga agcccctggt gggcgaggcc 4620
ctggcccgcg tgaagcgtgc tgagaagaag tggcgcgaca agcgcgagaa gctggagtgg 4680
gagaccaaca tcgtgtacaa ggaggccaag gagagcgtgg acgccctgtt cgtgaacagc 4740
cagtacgacc gcctgcaggc cgacaccaac atcgccatga tccacgccgc cgacaagcgc 4800
gtgcacagca ttcgcgaggc ctacctgccc gagctgagcg tgatccccgg tgtgaacgcc 4860
gccatcttcg aggaactcga gggccgcatc taggagctct atgatcgttc aaacatttgg 4920
caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt 4980
ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga 5040
tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata 5100
tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatc 5155
<210> 22
<211> 5152
<212> DNA
<213> 人工序列
<220>
<223> 24375 cry1Ab表达盒
<400> 22
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 60
agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 120
tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 180
tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 240
gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 300
ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 360
gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 420
agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 480
taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 540
aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 600
cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 660
cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 720
acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 780
ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 840
gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 900
ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 960
cccgtcggca cctccgcttc ctaggtacgc cgctcgtcct cccccccccc ccctctctac 1020
cttctctaga tcggcgttcc ggtccatagt tagggcccgg tagttctact tctgttcatg 1080
tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga 1140
cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg 1200
ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca 1260
tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt 1320
catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt 1380
ctagatcgga gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt 1440
atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc 1500
taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt 1560
tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt 1620
agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca 1680
tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca 1740
tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg 1800
ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tattttgatc 1860
ttgatatact tggatgatgg catatccagc agctatatgt ggattttttt agccctgcct 1920
tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg 1980
tgttacttct gcaggatcct ctaagtagca gccatggaca acaaccccaa catcaacgag 2040
tgcatcccct acaactgcct gagcaacccc gaggtggagg tgctgggcgg cgagcgcatc 2100
gagaccggct acacccccat cgacatcagc ctgagcctga cccagttcct gctgagcgag 2160
ttcgtgcccg gcgccggctt cgtgctgggc ctggtggaca tcatctgggg catcttcggc 2220
cccagccagt gggacgcctt cctggtgcag atcgagcagt tgataaacca acgcatagag 2280
gaattcgccc gcaaccaggc catcagccgc ctggagggcc tgagcaacct gtaccaaatc 2340
tacgccgaga gcttccgcga gtgggaggcc gaccccacca accccgccct gcgcgaggag 2400
atgcgcatcc agttcaacga catgaacagc gccctgacca ccgccatccc cctgttcgcc 2460
gtgcagaact accaggtgcc cctgctgagc gtgtacgtgc aggccgccaa cctgcacctg 2520
agcgtgctgc gcgacgtcag cgtgttcggc cagcgctggg gcttcgacgc cgccaccatc 2580
aacagccgct acaacgacct gacccgcctg atcggcaact acaccgacca cgccgtgcgc 2640
tggtacaaca ccggcctgga gcgcgtgtgg gggcccgaca gccgcgactg gatcaggtac 2700
aaccagttcc gccgcgagct gaccctgacc gtgctggaca tcgtgagcct gttccccaac 2760
tacgacagcc gcacctaccc catccgcacc gtgagccagc tgacccgcga gatttacacc 2820
aaccccgtgc tggagaactt cgacggcagc ttccgcggca gcgcccaggg catcgagggc 2880
agcatccgca gcccccacct gatggacatc ctgaacagca tcaccatcta caccgacgcc 2940
caccgcggcg agtactactg gagcggccac cagatcatgg ccagccccgt cggcttcagc 3000
ggccccgagt tcaccttccc cctgtacggc acgatgggca acgctgcacc tcagcagcgc 3060
atcgtggcac agctgggcca gggagtgtac cgcaccctga gcagcaccct gtaccgtcga 3120
cctttcaaca tcggcatcaa caaccagcag ctgagcgtgc tggacggcac cgagttcgcc 3180
tacggcacca gcagcaacct gcccagcgcc gtgtaccgca agagcggcac cgtggacagc 3240
ctggacgaga tcccccctca gaacaacaac gtgccacctc gacagggctt cagccaccgt 3300
ctgagccacg tgagcatgtt ccgcagtggc ttcagcaaca gcagcgtgag catcatccgt 3360
gcacctatgt tcagctggat tcaccgcagt gccgagttca acaacatcat ccccagcagc 3420
cagatcaccc agatccccct gaccaagagc accaacctgg gcagcggcac cagcgtggtg 3480
aagggccccg gcttcaccgg cggcgacatc ctgcgccgca ccagccccgg ccagatcagc 3540
accctgcgcg tgaacatcac cgcccccctg agccagcgct accgcgtccg catccgctac 3600
gccagcacca ccaacctgca gttccacacc agcatcgacg gccgccccat caaccagggc 3660
aacttcagcg ccaccatgag cagcggcagc aacctgcaga gcggcagctt ccgcaccgtg 3720
ggcttcacca cccccttcaa cttcagcaac ggcagcagcg tgttcaccct gagcgcccac 3780
gtgttcaaca gcggcaacga ggtgtacatc gaccgcatcg agttcgtgcc cgccgaggtg 3840
accttcgagg ccgagtacga cctggagagg gctcagaagg ccgtgaacga gctgttcacc 3900
agcagcaacc agatcggcct gaagaccgac gtgaccgact accacatcga ccaggtgagc 3960
aacctggtgg agtgcttaag cgacgagttc tgcctggacg agaagaagga gctgagcgag 4020
aaggtgaagc acgccaagcg cctgagcgac gagcgcaacc tgctgcagga ccccaacttc 4080
cgcggcatca accgccagct ggaccgcggc tggcgaggca gcaccgatat caccatccag 4140
ggcggcgacg acgtgttcaa ggagaactac gtgaccctgc tgggcacctt cgacgagtgc 4200
taccccacct acctgtacca gaagatcgac gagagcaagc tgaaggccta cacccgctac 4260
cagctgcgcg gctacatcga ggacagccag gacctggaaa tctacctgat ccgctacaac 4320
gcgaagcacg agaccgtgaa cgtgcccggc accggcagcc tgtggcccct gagcgccccc 4380
agccccatcg gcaagtgcac tagcatctct gttgcctttt tgattgaacc gtgccaacag 4440
cttgttggat ttactagtca ccacagccac cacttcagcc tggacatcga cgtgggctgc 4500
accgacctga acgaggacct gggcgtgtgg gtgatcttca agatcaagac ccaggacggc 4560
cacgcccgcc tgggcaatct agagttcctg gaggagaagc ccctggtggg cgaggccctg 4620
gcccgcgtga agcgtgctga gaagaagtgg cgcgacaagc gcgagaagct ggagtgggag 4680
accaacatcg tgtacaagga ggccaaggag agcgtggacg ccctgttcgt gaacagccag 4740
tacgaccgcc tgcaggccga caccaacatc gccatgatcc acgccgccga caagcgcgtg 4800
cacagcattc gcgaggccta cctgcccgag ctgagcgtga tccccggtgt gaacgccgcc 4860
atcttcgagg aactcgaggg ccgcatctag gagctctatg atcgttcaaa catttggcaa 4920
taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg 4980
ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg 5040
gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag 5100
cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tc 5152
<210> 23
<211> 5155
<212> DNA
<213> 人工序列
<220>
<223> 24376 cry1Ab表达盒
<400> 23
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 60
agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 120
tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 180
tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 240
gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 300
ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 360
gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 420
agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 480
taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 540
aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 600
cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 660
cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 720
acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 780
ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 840
gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 900
ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 960
cccgtcggca cctccgcttc ctaggtacgc cgctcgtcct cccccccccc ccctctctac 1020
cttctctaga tcggcgttcc ggtccatagt tagggcccgg tagttctact tctgttcatg 1080
tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga 1140
cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg 1200
ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca 1260
tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt 1320
catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt 1380
ctagatcgga gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt 1440
atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc 1500
taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt 1560
tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt 1620
agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca 1680
tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca 1740
tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg 1800
ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tattttgatc 1860
ttgatatact tggatgatgg catatccagc agctatatgt ggattttttt agccctgcct 1920
tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg 1980
tgttacttct gcaggatcct ctaagtagca gccatggaca acaaccccaa catcaacgag 2040
tgcatcccct acaactgcct gagcaacccc gaggtggagg tgctgggcgg cgagcgcatc 2100
gagaccggct acacccccat cgacatcagc ctgagcctga cccagttcct gctgagcgag 2160
ttcgtgcccg gcgccggctt cgtgctgggc ctggtggaca tcatctgggg catcttcggc 2220
cccagccagt gggacgcctt cctggtgcag atcgagcagt tgataaacca acgcatagag 2280
gaattcgccc gcaaccaggc catcagccgc ctggagggcc tgagcaacct gtaccaaatc 2340
tacgccgaga gcttccgcga gtgggaggcc gaccccacca accccgccct gcgcgaggag 2400
atgcgcatcc agttcaacga catgaacagc gccctgacca ccgccatccc cctgttcgcc 2460
gtgcagaact accaggtgcc cctgctgagc gtgtacgtgc aggccgccaa cctgcacctg 2520
agcgtgctgc gcgacgtcag cgtgttcggc cagcgctggg gcttcgacgc cgccaccatc 2580
aacagccgct acaacgacct gacccgcctg atcggcaact acaccgacca cgccgtgcgc 2640
tggtacaaca ccggcctgga gcgcgtgtgg gggcccgaca gccgcgactg gatcaggtac 2700
aaccagttcc gccgcgagct gaccctgacc gtgctggaca tcgtgagcct gttccccaac 2760
tacgacagcc gcacctaccc catccgcacc gtgagccagc tgacccgcga gatttacacc 2820
aaccccgtgc tggagaactt cgacggcagc ttccgcggca gcgcccaggg catcgagggc 2880
agcatccgca gcccccacct gatggacatc ctgaacagca tcaccatcta caccgacgcc 2940
caccgcggcg agtactactg gagcggccac cagatcatgg ccagccccgt cggcttcagc 3000
ggccccgagt tcaccttccc cctgtacggc acgatgggca acgctgcacc tcagcagcgc 3060
atcgtggcac agctgggcca gggagtgtac cgcaccctga gcagcaccct gtaccgtcga 3120
cctttcaaca tcggcatcaa caaccagcag ctgagcgtgc tggacggcac cgagttcgcc 3180
tacggcacca gcagcaacct gcccagcgcc gtgtaccgca agagcggcac cgtggacagc 3240
ctggacgaga tcccccctca gaacaacaac gtgccacctc gacagggctt cagccaccgt 3300
ctgagccacg tgagcatgtt ccgcagtggc ttcagcaaca gcagcgtgag catcatccgt 3360
gcacctatgt tcagctggat tcaccgcagt gccgagttca acaacatcat ccccagcagc 3420
cagatcaccc agatccccct gaccaagagc accaacctgg gcagcggcac cagcgtggtg 3480
aagggccccg gcttcaccgg cggcgacatc ctgcgccgca ccagccccgg ccagatcagc 3540
accctgcgcg tgaacatcac cgcccccctg agccagcgct accgcgtccg catccgctac 3600
gccagcacca ccaacctgca gttccacacc agcatcgacg gccgccccat caaccagggc 3660
aacttcagcg ccaccatgag cagcggcagc aacctgcaga gcggcagctt ccgcaccgtg 3720
ggcttcacca cccccttcaa cttcagcaac ggcagcagcg tgttcaccct gagcgcccac 3780
gtgttcaaca gcggcaacga ggtgtacatc gaccgcatcg agttcgtgcc cgccgaggtg 3840
accttcgagg ccgagtacga cctggagagg gctcagaagg ccgtgaacga gctgttcacc 3900
agcagcaacc agatcggcct gaagaccgac gtgaccgact accacatcga ccaggtgagc 3960
aacctggtgg agtgcttaag cgacgagttc tgcctggacg agaagaagga gctgagcgag 4020
aaggtgaagc acgccaagcg cctgagcgac gagcgcaacc tgctgcagga ccccaacttc 4080
cgcggcatca accgccagct ggaccgcggc tggcgaggca gcaccgatat caccatccag 4140
ggcggcgacg acgtgttcaa ggagaactac gtgaccctgc tgggcacctt cgacgagtgc 4200
taccccacct acctgtacca gaagatcgac gagagcaagc tgaaggccta cacccgctac 4260
cagctgcgcg gctacatcga ggacagccag gacctggaaa tctacctgat ccgctacaac 4320
gcgaagcacg agaccgtgaa cgtgcccggc accggcagcc tgtggcccct gagcgccccc 4380
agccccatcg gcaagtgcac tagcgacgac cccaaaggac aggtttccca cagctttatt 4440
gaacaattgt ttgcatctag tcaccacagc caccacttca gcctggacat cgacgtgggc 4500
tgcaccgacc tgaacgagga cctgggcgtg tgggtgatct tcaagatcaa gacccaggac 4560
ggccacgccc gcctgggcaa tctagagttc ctggaggaga agcccctggt gggcgaggcc 4620
ctggcccgcg tgaagcgtgc tgagaagaag tggcgcgaca agcgcgagaa gctggagtgg 4680
gagaccaaca tcgtgtacaa ggaggccaag gagagcgtgg acgccctgtt cgtgaacagc 4740
cagtacgacc gcctgcaggc cgacaccaac atcgccatga tccacgccgc cgacaagcgc 4800
gtgcacagca ttcgcgaggc ctacctgccc gagctgagcg tgatccccgg tgtgaacgcc 4860
gccatcttcg aggaactcga gggccgcatc taggagctct atgatcgttc aaacatttgg 4920
caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt 4980
ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga 5040
tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata 5100
tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatc 5155
<210> 24
<211> 5152
<212> DNA
<213> 人工序列
<220>
<223> 24377 cry1Ab表达盒
<400> 24
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 60
agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 120
tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 180
tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 240
gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 300
ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 360
gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 420
agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 480
taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 540
aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 600
cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 660
cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 720
acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 780
ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 840
gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 900
ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 960
cccgtcggca cctccgcttc ctaggtacgc cgctcgtcct cccccccccc ccctctctac 1020
cttctctaga tcggcgttcc ggtccatagt tagggcccgg tagttctact tctgttcatg 1080
tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga 1140
cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg 1200
ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca 1260
tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt 1320
catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt 1380
ctagatcgga gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt 1440
atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc 1500
taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt 1560
tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt 1620
agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca 1680
tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca 1740
tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg 1800
ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tattttgatc 1860
ttgatatact tggatgatgg catatccagc agctatatgt ggattttttt agccctgcct 1920
tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg 1980
tgttacttct gcaggatcct ctaagtagca gccatggaca acaaccccaa catcaacgag 2040
tgcatcccct acaactgcct gagcaacccc gaggtggagg tgctgggcgg cgagcgcatc 2100
gagaccggct acacccccat cgacatcagc ctgagcctga cccagttcct gctgagcgag 2160
ttcgtgcccg gcgccggctt cgtgctgggc ctggtggaca tcatctgggg catcttcggc 2220
cccagccagt gggacgcctt cctggtgcag atcgagcagt tgataaacca acgcatagag 2280
gaattcgccc gcaaccaggc catcagccgc ctggagggcc tgagcaacct gtaccaaatc 2340
tacgccgaga gcttccgcga gtgggaggcc gaccccacca accccgccct gcgcgaggag 2400
atgcgcatcc agttcaacga catgaacagc gccctgacca ccgccatccc cctgttcgcc 2460
gtgcagaact accaggtgcc cctgctgagc gtgtacgtgc aggccgccaa cctgcacctg 2520
agcgtgctgc gcgacgtcag cgtgttcggc cagcgctggg gcttcgacgc cgccaccatc 2580
aacagccgct acaacgacct gacccgcctg atcggcaact acaccgacca cgccgtgcgc 2640
tggtacaaca ccggcctgga gcgcgtgtgg gggcccgaca gccgcgactg gatcaggtac 2700
aaccagttcc gccgcgagct gaccctgacc gtgctggaca tcgtgagcct gttccccaac 2760
tacgacagcc gcacctaccc catccgcacc gtgagccagc tgacccgcga gatttacacc 2820
aaccccgtgc tggagaactt cgacggcagc ttccgcggca gcgcccaggg catcgagggc 2880
agcatccgca gcccccacct gatggacatc ctgaacagca tcaccatcta caccgacgcc 2940
caccgcggcg agtactactg gagcggccac cagatcatgg ccagccccgt cggcttcagc 3000
ggccccgagt tcaccttccc cctgtacggc acgatgggca acgctgcacc tcagcagcgc 3060
atcgtggcac agctgggcca gggagtgtac cgcaccctga gcagcaccct gtaccgtcga 3120
cctttcaaca tcggcatcaa caaccagcag ctgagcgtgc tggacggcac cgagttcgcc 3180
tacggcacca gcagcaacct gcccagcgcc gtgtaccgca agagcggcac cgtggacagc 3240
ctggacgaga tcccccctca gaacaacaac gtgccacctc gacagggctt cagccaccgt 3300
ctgagccacg tgagcatgtt ccgcagtggc ttcagcaaca gcagcgtgag catcatccgt 3360
gcacctatgt tcagctggat tcaccgcagt gccgagttca acaacatcat ccccagcagc 3420
cagatcaccc agatccccct gaccaagagc accaacctgg gcagcggcac cagcgtggtg 3480
aagggccccg gcttcaccgg cggcgacatc ctgcgccgca ccagccccgg ccagatcagc 3540
accctgcgcg tgaacatcac cgcccccctg agccagcgct accgcgtccg catccgctac 3600
gccagcacca ccaacctgca gttccacacc agcatcgacg gccgccccat caaccagggc 3660
aacttcagcg ccaccatgag cagcggcagc aacctgcaga gcggcagctt ccgcaccgtg 3720
ggcttcacca cccccttcaa cttcagcaac ggcagcagcg tgttcaccct gagcgcccac 3780
gtgttcaaca gcggcaacga ggtgtacatc gaccgcatcg agttcgtgcc cgccgaggtg 3840
accttcgagg ccgagtacga cctggagagg gctcagaagg ccgtgaacga gctgttcacc 3900
agcagcaacc agatcggcct gaagaccgac gtgaccgact accacatcga ccaggtgagc 3960
aacctggtgg agtgcttaag cgacgagttc tgcctggacg agaagaagga gctgagcgag 4020
aaggtgaagc acgccaagcg cctgagcgac gagcgcaacc tgctgcagga ccccaacttc 4080
cgcggcatca accgccagct ggaccgcggc tggcgaggca gcaccgatat caccatccag 4140
ggcggcgacg acgtgttcaa ggagaactac gtgaccctgc tgggcacctt cgacgagtgc 4200
taccccacct acctgtacca gaagatcgac gagagcaagc tgaaggccta cacccgctac 4260
cagctgcgcg gctacatcga ggacagccag gacctggaaa tctacctgat ccgctacaac 4320
gcgaagcacg agaccgtgaa cgtgcccggc accggcagcc tgtggcccct gagcgccccc 4380
agccccatcg gcaagtgcac tagtcctttc tctctctttc tttctctctg ctttattgaa 4440
cttctcaaag ttactagtca ccacagccac cacttcagcc tggacatcga cgtgggctgc 4500
accgacctga acgaggacct gggcgtgtgg gtgatcttca agatcaagac ccaggacggc 4560
cacgcccgcc tgggcaatct agagttcctg gaggagaagc ccctggtggg cgaggccctg 4620
gcccgcgtga agcgtgctga gaagaagtgg cgcgacaagc gcgagaagct ggagtgggag 4680
accaacatcg tgtacaagga ggccaaggag agcgtggacg ccctgttcgt gaacagccag 4740
tacgaccgcc tgcaggccga caccaacatc gccatgatcc acgccgccga caagcgcgtg 4800
cacagcattc gcgaggccta cctgcccgag ctgagcgtga tccccggtgt gaacgccgcc 4860
atcttcgagg aactcgaggg ccgcatctag gagctctatg atcgttcaaa catttggcaa 4920
taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg 4980
ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg 5040
gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag 5100
cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tc 5152
<210> 25
<211> 5155
<212> DNA
<213> 人工序列
<220>
<223> 24379 cry1Ab表达盒
<400> 25
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 60
agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 120
tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 180
tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 240
gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 300
ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 360
gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 420
agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 480
taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 540
aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 600
cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 660
cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 720
acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 780
ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 840
gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 900
ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 960
cccgtcggca cctccgcttc ctagcatcct tttcattgat aagaagagca ccgttcactc 1020
caactgcaaa cttgtggcta ggtacgccgc tcgtcctccc cccccccccc tctctacctt 1080
ctctagatcg gcgttccggt ccatagttag ggcccggtag ttctacttct gttcatgttt 1140
gtgttagatc cgtgtttgtg ttagatccgt gctgctagcg ttcgtacacg gatgcgacct 1200
gtacgtcaga cacgttctga ttgctaactt gccagtgttt ctctttgggg aatcctggga 1260
tggctctagc cgttccgcag acgggatcga tttcatgatt ttttttgttt cgttgcatag 1320
ggtttggttt gcccttttcc tttatttcaa tatatgccgt gcacttgttt gtcgggtcat 1380
cttttcatgc ttttttttgt cttggttgtg atgatgtggt ctggttgggc ggtcgttcta 1440
gatcggagta gaattctgtt tcaaactacc tggtggattt attaattttg gatctgtatg 1500
tgtgtgccat acatattcat agttacgaat tgaagatgat ggatggaaat atcgatctag 1560
gataggtata catgttgatg cgggttttac tgatgcatat acagagatgc tttttgttcg 1620
cttggttgtg atgatgtggt gtggttgggc ggtcgttcat tcgttctaga tcggagtaga 1680
atactgtttc aaactacctg gtgtatttat taattttgga actgtatgtg tgtgtcatac 1740
atcttcatag ttacgagttt aagatggatg gaaatatcga tctaggatag gtatacatgt 1800
tgatgtgggt tttactgatg catatacatg atggcatatg cagcatctat tcatatgctc 1860
taaccttgag tacctatcta ttataataaa caagtatgtt ttataattat tttgatcttg 1920
atatacttgg atgatggcat atccagcagc tatatgtgga tttttttagc cctgccttca 1980
tacgctattt atttgcttgg tactgtttct tttgtcgatg ctcaccctgt tgtttggtgt 2040
tacttctgca ggatcctcta agtagcagcc atggacaaca accccaacat caacgagtgc 2100
atcccctaca actgcctgag caaccccgag gtggaggtgc tgggcggcga gcgcatcgag 2160
accggctaca cccccatcga catcagcctg agcctgaccc agttcctgct gagcgagttc 2220
gtgcccggcg ccggcttcgt gctgggcctg gtggacatca tctggggcat cttcggcccc 2280
agccagtggg acgccttcct ggtgcagatc gagcagttga taaaccaacg catagaggaa 2340
ttcgcccgca accaggccat cagccgcctg gagggcctga gcaacctgta ccaaatctac 2400
gccgagagct tccgcgagtg ggaggccgac cccaccaacc ccgccctgcg cgaggagatg 2460
cgcatccagt tcaacgacat gaacagcgcc ctgaccaccg ccatccccct gttcgccgtg 2520
cagaactacc aggtgcccct gctgagcgtg tacgtgcagg ccgccaacct gcacctgagc 2580
gtgctgcgcg acgtcagcgt gttcggccag cgctggggct tcgacgccgc caccatcaac 2640
agccgctaca acgacctgac ccgcctgatc ggcaactaca ccgaccacgc cgtgcgctgg 2700
tacaacaccg gcctggagcg cgtgtggggg cccgacagcc gcgactggat caggtacaac 2760
cagttccgcc gcgagctgac cctgaccgtg ctggacatcg tgagcctgtt ccccaactac 2820
gacagccgca cctaccccat ccgcaccgtg agccagctga cccgcgagat ttacaccaac 2880
cccgtgctgg agaacttcga cggcagcttc cgcggcagcg cccagggcat cgagggcagc 2940
atccgcagcc cccacctgat ggacatcctg aacagcatca ccatctacac cgacgcccac 3000
cgcggcgagt actactggag cggccaccag atcatggcca gccccgtcgg cttcagcggc 3060
cccgagttca ccttccccct gtacggcacg atgggcaacg ctgcacctca gcagcgcatc 3120
gtggcacagc tgggccaggg agtgtaccgc accctgagca gcaccctgta ccgtcgacct 3180
ttcaacatcg gcatcaacaa ccagcagctg agcgtgctgg acggcaccga gttcgcctac 3240
ggcaccagca gcaacctgcc cagcgccgtg taccgcaaga gcggcaccgt ggacagcctg 3300
gacgagatcc cccctcagaa caacaacgtg ccacctcgac agggcttcag ccaccgtctg 3360
agccacgtga gcatgttccg cagtggcttc agcaacagca gcgtgagcat catccgtgca 3420
cctatgttca gctggattca ccgcagtgcc gagttcaaca acatcatccc cagcagccag 3480
atcacccaga tccccctgac caagagcacc aacctgggca gcggcaccag cgtggtgaag 3540
ggccccggct tcaccggcgg cgacatcctg cgccgcacca gccccggcca gatcagcacc 3600
ctgcgcgtga acatcaccgc ccccctgagc cagcgctacc gcgtccgcat ccgctacgcc 3660
agcaccacca acctgcagtt ccacaccagc atcgacggcc gccccatcaa ccagggcaac 3720
ttcagcgcca ccatgagcag cggcagcaac ctgcagagcg gcagcttccg caccgtgggc 3780
ttcaccaccc ccttcaactt cagcaacggc agcagcgtgt tcaccctgag cgcccacgtg 3840
ttcaacagcg gcaacgaggt gtacatcgac cgcatcgagt tcgtgcccgc cgaggtgacc 3900
ttcgaggccg agtacgacct ggagagggct cagaaggccg tgaacgagct gttcaccagc 3960
agcaaccaga tcggcctgaa gaccgacgtg accgactacc acatcgacca ggtgagcaac 4020
ctggtggagt gcttaagcga cgagttctgc ctggacgaga agaaggagct gagcgagaag 4080
gtgaagcacg ccaagcgcct gagcgacgag cgcaacctgc tgcaggaccc caacttccgc 4140
ggcatcaacc gccagctgga ccgcggctgg cgaggcagca ccgatatcac catccagggc 4200
ggcgacgacg tgttcaagga gaactacgtg accctgctgg gcaccttcga cgagtgctac 4260
cccacctacc tgtaccagaa gatcgacgag agcaagctga aggcctacac ccgctaccag 4320
ctgcgcggct acatcgagga cagccaggac ctggaaatct acctgatccg ctacaacgcg 4380
aagcacgaga ccgtgaacgt gcccggcacc ggcagcctgt ggcccctgag cgcccccagc 4440
cccatcggca agtgcactag tcaccacagc caccacttca gcctggacat cgacgtgggc 4500
tgcaccgacc tgaacgagga cctgggcgtg tgggtgatct tcaagatcaa gacccaggac 4560
ggccacgccc gcctgggcaa tctagagttc ctggaggaga agcccctggt gggcgaggcc 4620
ctggcccgcg tgaagcgtgc tgagaagaag tggcgcgaca agcgcgagaa gctggagtgg 4680
gagaccaaca tcgtgtacaa ggaggccaag gagagcgtgg acgccctgtt cgtgaacagc 4740
cagtacgacc gcctgcaggc cgacaccaac atcgccatga tccacgccgc cgacaagcgc 4800
gtgcacagca ttcgcgaggc ctacctgccc gagctgagcg tgatccccgg tgtgaacgcc 4860
gccatcttcg aggaactcga gggccgcatc taggagctct atgatcgttc aaacatttgg 4920
caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt 4980
ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga 5040
tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata 5100
tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatc 5155
<210> 26
<211> 5154
<212> DNA
<213> 人工序列
<220>
<223> 24380 cry1Ab表达盒
<400> 26
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 60
agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 120
tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 180
tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 240
gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 300
ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 360
gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 420
agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 480
taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 540
aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 600
cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 660
cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 720
acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 780
ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 840
gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 900
ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 960
cccgtcggca cctccgcttc ctagacatcc aacacgcacg agatgacaga tagagcagtg 1020
agctgaccat ctcttactag gtacgccgct cgtcctcccc ccccccccct ctctaccttc 1080
tctagatcgg cgttccggtc catagttagg gcccggtagt tctacttctg ttcatgtttg 1140
tgttagatcc gtgtttgtgt tagatccgtg ctgctagcgt tcgtacacgg atgcgacctg 1200
tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga atcctgggat 1260
ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc gttgcatagg 1320
gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg tcgggtcatc 1380
ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg gtcgttctag 1440
atcggagtag aattctgttt caaactacct ggtggattta ttaattttgg atctgtatgt 1500
gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata tcgatctagg 1560
ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct ttttgttcgc 1620
ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat cggagtagaa 1680
tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt gtgtcataca 1740
tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg tatacatgtt 1800
gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt catatgctct 1860
aaccttgagt acctatctat tataataaac aagtatgttt tataattatt ttgatcttga 1920
tatacttgga tgatggcata tccagcagct atatgtggat ttttttagcc ctgccttcat 1980
acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt gtttggtgtt 2040
acttctgcag gatcctctaa gtagcagcca tggacaacaa ccccaacatc aacgagtgca 2100
tcccctacaa ctgcctgagc aaccccgagg tggaggtgct gggcggcgag cgcatcgaga 2160
ccggctacac ccccatcgac atcagcctga gcctgaccca gttcctgctg agcgagttcg 2220
tgcccggcgc cggcttcgtg ctgggcctgg tggacatcat ctggggcatc ttcggcccca 2280
gccagtggga cgccttcctg gtgcagatcg agcagttgat aaaccaacgc atagaggaat 2340
tcgcccgcaa ccaggccatc agccgcctgg agggcctgag caacctgtac caaatctacg 2400
ccgagagctt ccgcgagtgg gaggccgacc ccaccaaccc cgccctgcgc gaggagatgc 2460
gcatccagtt caacgacatg aacagcgccc tgaccaccgc catccccctg ttcgccgtgc 2520
agaactacca ggtgcccctg ctgagcgtgt acgtgcaggc cgccaacctg cacctgagcg 2580
tgctgcgcga cgtcagcgtg ttcggccagc gctggggctt cgacgccgcc accatcaaca 2640
gccgctacaa cgacctgacc cgcctgatcg gcaactacac cgaccacgcc gtgcgctggt 2700
acaacaccgg cctggagcgc gtgtgggggc ccgacagccg cgactggatc aggtacaacc 2760
agttccgccg cgagctgacc ctgaccgtgc tggacatcgt gagcctgttc cccaactacg 2820
acagccgcac ctaccccatc cgcaccgtga gccagctgac ccgcgagatt tacaccaacc 2880
ccgtgctgga gaacttcgac ggcagcttcc gcggcagcgc ccagggcatc gagggcagca 2940
tccgcagccc ccacctgatg gacatcctga acagcatcac catctacacc gacgcccacc 3000
gcggcgagta ctactggagc ggccaccaga tcatggccag ccccgtcggc ttcagcggcc 3060
ccgagttcac cttccccctg tacggcacga tgggcaacgc tgcacctcag cagcgcatcg 3120
tggcacagct gggccaggga gtgtaccgca ccctgagcag caccctgtac cgtcgacctt 3180
tcaacatcgg catcaacaac cagcagctga gcgtgctgga cggcaccgag ttcgcctacg 3240
gcaccagcag caacctgccc agcgccgtgt accgcaagag cggcaccgtg gacagcctgg 3300
acgagatccc ccctcagaac aacaacgtgc cacctcgaca gggcttcagc caccgtctga 3360
gccacgtgag catgttccgc agtggcttca gcaacagcag cgtgagcatc atccgtgcac 3420
ctatgttcag ctggattcac cgcagtgccg agttcaacaa catcatcccc agcagccaga 3480
tcacccagat ccccctgacc aagagcacca acctgggcag cggcaccagc gtggtgaagg 3540
gccccggctt caccggcggc gacatcctgc gccgcaccag ccccggccag atcagcaccc 3600
tgcgcgtgaa catcaccgcc cccctgagcc agcgctaccg cgtccgcatc cgctacgcca 3660
gcaccaccaa cctgcagttc cacaccagca tcgacggccg ccccatcaac cagggcaact 3720
tcagcgccac catgagcagc ggcagcaacc tgcagagcgg cagcttccgc accgtgggct 3780
tcaccacccc cttcaacttc agcaacggca gcagcgtgtt caccctgagc gcccacgtgt 3840
tcaacagcgg caacgaggtg tacatcgacc gcatcgagtt cgtgcccgcc gaggtgacct 3900
tcgaggccga gtacgacctg gagagggctc agaaggccgt gaacgagctg ttcaccagca 3960
gcaaccagat cggcctgaag accgacgtga ccgactacca catcgaccag gtgagcaacc 4020
tggtggagtg cttaagcgac gagttctgcc tggacgagaa gaaggagctg agcgagaagg 4080
tgaagcacgc caagcgcctg agcgacgagc gcaacctgct gcaggacccc aacttccgcg 4140
gcatcaaccg ccagctggac cgcggctggc gaggcagcac cgatatcacc atccagggcg 4200
gcgacgacgt gttcaaggag aactacgtga ccctgctggg caccttcgac gagtgctacc 4260
ccacctacct gtaccagaag atcgacgaga gcaagctgaa ggcctacacc cgctaccagc 4320
tgcgcggcta catcgaggac agccaggacc tggaaatcta cctgatccgc tacaacgcga 4380
agcacgagac cgtgaacgtg cccggcaccg gcagcctgtg gcccctgagc gcccccagcc 4440
ccatcggcaa gtgcactagt caccacagcc accacttcag cctggacatc gacgtgggct 4500
gcaccgacct gaacgaggac ctgggcgtgt gggtgatctt caagatcaag acccaggacg 4560
gccacgcccg cctgggcaat ctagagttcc tggaggagaa gcccctggtg ggcgaggccc 4620
tggcccgcgt gaagcgtgct gagaagaagt ggcgcgacaa gcgcgagaag ctggagtggg 4680
agaccaacat cgtgtacaag gaggccaagg agagcgtgga cgccctgttc gtgaacagcc 4740
agtacgaccg cctgcaggcc gacaccaaca tcgccatgat ccacgccgcc gacaagcgcg 4800
tgcacagcat tcgcgaggcc tacctgcccg agctgagcgt gatccccggt gtgaacgccg 4860
ccatcttcga ggaactcgag ggccgcatct aggagctcta tgatcgttca aacatttggc 4920
aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc 4980
tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat 5040
gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat 5100
agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatc 5154
<210> 27
<211> 5152
<212> DNA
<213> 人工序列
<220>
<223> 24381 cry1Ab表达盒
<400> 27
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 60
agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 120
tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 180
tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 240
gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 300
ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 360
gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 420
agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 480
taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 540
aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 600
cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 660
cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 720
acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 780
ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 840
gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 900
ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 960
cccgtcggca cctccgcttc ctagcatctc tgttgccttt ttgattgaac cgtgccaaca 1020
gcttgttgga tttactaggt acgccgctcg tcctcccccc ccccccctct ctaccttctc 1080
tagatcggcg ttccggtcca tagttagggc ccggtagttc tacttctgtt catgtttgtg 1140
ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat gcgacctgta 1200
cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat cctgggatgg 1260
ctctagccgt tccgcagacg ggatcgattt catgattttt tttgtttcgt tgcatagggt 1320
ttggtttgcc cttttccttt atttcaatat atgccgtgca cttgtttgtc gggtcatctt 1380
ttcatgcttt tttttgtctt ggttgtgatg atgtggtctg gttgggcggt cgttctagat 1440
cggagtagaa ttctgtttca aactacctgg tggatttatt aattttggat ctgtatgtgt 1500
gtgccataca tattcatagt tacgaattga agatgatgga tggaaatatc gatctaggat 1560
aggtatacat gttgatgcgg gttttactga tgcatataca gagatgcttt ttgttcgctt 1620
ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg ttctagatcg gagtagaata 1680
ctgtttcaaa ctacctggtg tatttattaa ttttggaact gtatgtgtgt gtcatacatc 1740
ttcatagtta cgagtttaag atggatggaa atatcgatct aggataggta tacatgttga 1800
tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca tatgctctaa 1860
ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt gatcttgata 1920
tacttggatg atggcatatc cagcagctat atgtggattt ttttagccct gccttcatac 1980
gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt ttggtgttac 2040
ttctgcagga tcctctaagt agcagccatg gacaacaacc ccaacatcaa cgagtgcatc 2100
ccctacaact gcctgagcaa ccccgaggtg gaggtgctgg gcggcgagcg catcgagacc 2160
ggctacaccc ccatcgacat cagcctgagc ctgacccagt tcctgctgag cgagttcgtg 2220
cccggcgccg gcttcgtgct gggcctggtg gacatcatct ggggcatctt cggccccagc 2280
cagtgggacg ccttcctggt gcagatcgag cagttgataa accaacgcat agaggaattc 2340
gcccgcaacc aggccatcag ccgcctggag ggcctgagca acctgtacca aatctacgcc 2400
gagagcttcc gcgagtggga ggccgacccc accaaccccg ccctgcgcga ggagatgcgc 2460
atccagttca acgacatgaa cagcgccctg accaccgcca tccccctgtt cgccgtgcag 2520
aactaccagg tgcccctgct gagcgtgtac gtgcaggccg ccaacctgca cctgagcgtg 2580
ctgcgcgacg tcagcgtgtt cggccagcgc tggggcttcg acgccgccac catcaacagc 2640
cgctacaacg acctgacccg cctgatcggc aactacaccg accacgccgt gcgctggtac 2700
aacaccggcc tggagcgcgt gtgggggccc gacagccgcg actggatcag gtacaaccag 2760
ttccgccgcg agctgaccct gaccgtgctg gacatcgtga gcctgttccc caactacgac 2820
agccgcacct accccatccg caccgtgagc cagctgaccc gcgagattta caccaacccc 2880
gtgctggaga acttcgacgg cagcttccgc ggcagcgccc agggcatcga gggcagcatc 2940
cgcagccccc acctgatgga catcctgaac agcatcacca tctacaccga cgcccaccgc 3000
ggcgagtact actggagcgg ccaccagatc atggccagcc ccgtcggctt cagcggcccc 3060
gagttcacct tccccctgta cggcacgatg ggcaacgctg cacctcagca gcgcatcgtg 3120
gcacagctgg gccagggagt gtaccgcacc ctgagcagca ccctgtaccg tcgacctttc 3180
aacatcggca tcaacaacca gcagctgagc gtgctggacg gcaccgagtt cgcctacggc 3240
accagcagca acctgcccag cgccgtgtac cgcaagagcg gcaccgtgga cagcctggac 3300
gagatccccc ctcagaacaa caacgtgcca cctcgacagg gcttcagcca ccgtctgagc 3360
cacgtgagca tgttccgcag tggcttcagc aacagcagcg tgagcatcat ccgtgcacct 3420
atgttcagct ggattcaccg cagtgccgag ttcaacaaca tcatccccag cagccagatc 3480
acccagatcc ccctgaccaa gagcaccaac ctgggcagcg gcaccagcgt ggtgaagggc 3540
cccggcttca ccggcggcga catcctgcgc cgcaccagcc ccggccagat cagcaccctg 3600
cgcgtgaaca tcaccgcccc cctgagccag cgctaccgcg tccgcatccg ctacgccagc 3660
accaccaacc tgcagttcca caccagcatc gacggccgcc ccatcaacca gggcaacttc 3720
agcgccacca tgagcagcgg cagcaacctg cagagcggca gcttccgcac cgtgggcttc 3780
accaccccct tcaacttcag caacggcagc agcgtgttca ccctgagcgc ccacgtgttc 3840
aacagcggca acgaggtgta catcgaccgc atcgagttcg tgcccgccga ggtgaccttc 3900
gaggccgagt acgacctgga gagggctcag aaggccgtga acgagctgtt caccagcagc 3960
aaccagatcg gcctgaagac cgacgtgacc gactaccaca tcgaccaggt gagcaacctg 4020
gtggagtgct taagcgacga gttctgcctg gacgagaaga aggagctgag cgagaaggtg 4080
aagcacgcca agcgcctgag cgacgagcgc aacctgctgc aggaccccaa cttccgcggc 4140
atcaaccgcc agctggaccg cggctggcga ggcagcaccg atatcaccat ccagggcggc 4200
gacgacgtgt tcaaggagaa ctacgtgacc ctgctgggca ccttcgacga gtgctacccc 4260
acctacctgt accagaagat cgacgagagc aagctgaagg cctacacccg ctaccagctg 4320
cgcggctaca tcgaggacag ccaggacctg gaaatctacc tgatccgcta caacgcgaag 4380
cacgagaccg tgaacgtgcc cggcaccggc agcctgtggc ccctgagcgc ccccagcccc 4440
atcggcaagt gcactagtca ccacagccac cacttcagcc tggacatcga cgtgggctgc 4500
accgacctga acgaggacct gggcgtgtgg gtgatcttca agatcaagac ccaggacggc 4560
cacgcccgcc tgggcaatct agagttcctg gaggagaagc ccctggtggg cgaggccctg 4620
gcccgcgtga agcgtgctga gaagaagtgg cgcgacaagc gcgagaagct ggagtgggag 4680
accaacatcg tgtacaagga ggccaaggag agcgtggacg ccctgttcgt gaacagccag 4740
tacgaccgcc tgcaggccga caccaacatc gccatgatcc acgccgccga caagcgcgtg 4800
cacagcattc gcgaggccta cctgcccgag ctgagcgtga tccccggtgt gaacgccgcc 4860
atcttcgagg aactcgaggg ccgcatctag gagctctatg atcgttcaaa catttggcaa 4920
taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg 4980
ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg 5040
gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag 5100
cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tc 5152
<210> 28
<211> 5152
<212> DNA
<213> 人工序列
<220>
<223> 24383 cry1Ab表达盒
<400> 28
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 60
agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 120
tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 180
tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 240
gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 300
ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 360
gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 420
agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 480
taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 540
aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 600
cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 660
cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 720
acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 780
ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 840
gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 900
ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 960
cccgtcggca cctccgcttc ctagtccttt ctctctcttt ctttctctct gctttattga 1020
acttctcaaa gttactaggt acgccgctcg tcctcccccc ccccccctct ctaccttctc 1080
tagatcggcg ttccggtcca tagttagggc ccggtagttc tacttctgtt catgtttgtg 1140
ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat gcgacctgta 1200
cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat cctgggatgg 1260
ctctagccgt tccgcagacg ggatcgattt catgattttt tttgtttcgt tgcatagggt 1320
ttggtttgcc cttttccttt atttcaatat atgccgtgca cttgtttgtc gggtcatctt 1380
ttcatgcttt tttttgtctt ggttgtgatg atgtggtctg gttgggcggt cgttctagat 1440
cggagtagaa ttctgtttca aactacctgg tggatttatt aattttggat ctgtatgtgt 1500
gtgccataca tattcatagt tacgaattga agatgatgga tggaaatatc gatctaggat 1560
aggtatacat gttgatgcgg gttttactga tgcatataca gagatgcttt ttgttcgctt 1620
ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg ttctagatcg gagtagaata 1680
ctgtttcaaa ctacctggtg tatttattaa ttttggaact gtatgtgtgt gtcatacatc 1740
ttcatagtta cgagtttaag atggatggaa atatcgatct aggataggta tacatgttga 1800
tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca tatgctctaa 1860
ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt gatcttgata 1920
tacttggatg atggcatatc cagcagctat atgtggattt ttttagccct gccttcatac 1980
gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt ttggtgttac 2040
ttctgcagga tcctctaagt agcagccatg gacaacaacc ccaacatcaa cgagtgcatc 2100
ccctacaact gcctgagcaa ccccgaggtg gaggtgctgg gcggcgagcg catcgagacc 2160
ggctacaccc ccatcgacat cagcctgagc ctgacccagt tcctgctgag cgagttcgtg 2220
cccggcgccg gcttcgtgct gggcctggtg gacatcatct ggggcatctt cggccccagc 2280
cagtgggacg ccttcctggt gcagatcgag cagttgataa accaacgcat agaggaattc 2340
gcccgcaacc aggccatcag ccgcctggag ggcctgagca acctgtacca aatctacgcc 2400
gagagcttcc gcgagtggga ggccgacccc accaaccccg ccctgcgcga ggagatgcgc 2460
atccagttca acgacatgaa cagcgccctg accaccgcca tccccctgtt cgccgtgcag 2520
aactaccagg tgcccctgct gagcgtgtac gtgcaggccg ccaacctgca cctgagcgtg 2580
ctgcgcgacg tcagcgtgtt cggccagcgc tggggcttcg acgccgccac catcaacagc 2640
cgctacaacg acctgacccg cctgatcggc aactacaccg accacgccgt gcgctggtac 2700
aacaccggcc tggagcgcgt gtgggggccc gacagccgcg actggatcag gtacaaccag 2760
ttccgccgcg agctgaccct gaccgtgctg gacatcgtga gcctgttccc caactacgac 2820
agccgcacct accccatccg caccgtgagc cagctgaccc gcgagattta caccaacccc 2880
gtgctggaga acttcgacgg cagcttccgc ggcagcgccc agggcatcga gggcagcatc 2940
cgcagccccc acctgatgga catcctgaac agcatcacca tctacaccga cgcccaccgc 3000
ggcgagtact actggagcgg ccaccagatc atggccagcc ccgtcggctt cagcggcccc 3060
gagttcacct tccccctgta cggcacgatg ggcaacgctg cacctcagca gcgcatcgtg 3120
gcacagctgg gccagggagt gtaccgcacc ctgagcagca ccctgtaccg tcgacctttc 3180
aacatcggca tcaacaacca gcagctgagc gtgctggacg gcaccgagtt cgcctacggc 3240
accagcagca acctgcccag cgccgtgtac cgcaagagcg gcaccgtgga cagcctggac 3300
gagatccccc ctcagaacaa caacgtgcca cctcgacagg gcttcagcca ccgtctgagc 3360
cacgtgagca tgttccgcag tggcttcagc aacagcagcg tgagcatcat ccgtgcacct 3420
atgttcagct ggattcaccg cagtgccgag ttcaacaaca tcatccccag cagccagatc 3480
acccagatcc ccctgaccaa gagcaccaac ctgggcagcg gcaccagcgt ggtgaagggc 3540
cccggcttca ccggcggcga catcctgcgc cgcaccagcc ccggccagat cagcaccctg 3600
cgcgtgaaca tcaccgcccc cctgagccag cgctaccgcg tccgcatccg ctacgccagc 3660
accaccaacc tgcagttcca caccagcatc gacggccgcc ccatcaacca gggcaacttc 3720
agcgccacca tgagcagcgg cagcaacctg cagagcggca gcttccgcac cgtgggcttc 3780
accaccccct tcaacttcag caacggcagc agcgtgttca ccctgagcgc ccacgtgttc 3840
aacagcggca acgaggtgta catcgaccgc atcgagttcg tgcccgccga ggtgaccttc 3900
gaggccgagt acgacctgga gagggctcag aaggccgtga acgagctgtt caccagcagc 3960
aaccagatcg gcctgaagac cgacgtgacc gactaccaca tcgaccaggt gagcaacctg 4020
gtggagtgct taagcgacga gttctgcctg gacgagaaga aggagctgag cgagaaggtg 4080
aagcacgcca agcgcctgag cgacgagcgc aacctgctgc aggaccccaa cttccgcggc 4140
atcaaccgcc agctggaccg cggctggcga ggcagcaccg atatcaccat ccagggcggc 4200
gacgacgtgt tcaaggagaa ctacgtgacc ctgctgggca ccttcgacga gtgctacccc 4260
acctacctgt accagaagat cgacgagagc aagctgaagg cctacacccg ctaccagctg 4320
cgcggctaca tcgaggacag ccaggacctg gaaatctacc tgatccgcta caacgcgaag 4380
cacgagaccg tgaacgtgcc cggcaccggc agcctgtggc ccctgagcgc ccccagcccc 4440
atcggcaagt gcactagtca ccacagccac cacttcagcc tggacatcga cgtgggctgc 4500
accgacctga acgaggacct gggcgtgtgg gtgatcttca agatcaagac ccaggacggc 4560
cacgcccgcc tgggcaatct agagttcctg gaggagaagc ccctggtggg cgaggccctg 4620
gcccgcgtga agcgtgctga gaagaagtgg cgcgacaagc gcgagaagct ggagtgggag 4680
accaacatcg tgtacaagga ggccaaggag agcgtggacg ccctgttcgt gaacagccag 4740
tacgaccgcc tgcaggccga caccaacatc gccatgatcc acgccgccga caagcgcgtg 4800
cacagcattc gcgaggccta cctgcccgag ctgagcgtga tccccggtgt gaacgccgcc 4860
atcttcgagg aactcgaggg ccgcatctag gagctctatg atcgttcaaa catttggcaa 4920
taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg 4980
ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg 5040
gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag 5100
cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tc 5152
<210> 29
<211> 21
<212> RNA
<213> 玉蜀黍
<400> 29
cagagcuccc uucacuccaa a 21
<210> 30
<211> 21
<212> RNA
<213> 人工序列
<220>
<223> 突变型miR159h-3p起始子序列
<400> 30
aagagcaccg uucacuccaa c 21
<210> 31
<211> 22
<212> RNA
<213> 玉蜀黍
<400> 31
gaugacagau agagcaguga gc 22
<210> 32
<211> 21
<212> RNA
<213> 玉蜀黍
<400> 32
uuugauugaa ccgugccaac a 21
<210> 33
<211> 21
<212> RNA
<213> 玉蜀黍
<400> 33
uuucccacag cuuuauugaa c 21
<210> 34
<211> 21
<212> RNA
<213> 人工序列
<220>
<223> miR396b-3p突变体
<400> 34
uuucucucug cuuuauugaa c 21
<210> 35
<211> 21
<212> RNA
<213> 人工序列
<220>
<223> 突变型miR159h-3p
<400> 35
guuggaguga acggugcucu u 21
<210> 36
<211> 21
<212> RNA
<213> 玉蜀黍
<400> 36
uuuggaguga agggagcucu g 21
<210> 37
<211> 22
<212> RNA
<213> 玉蜀黍
<400> 37
gcucacugcu cuaucuguca uc 22
<210> 38
<211> 21
<212> RNA
<213> 玉蜀黍
<400> 38
uguuggcacg guucaaucaa a 21
<210> 39
<211> 21
<212> RNA
<213> 玉蜀黍
<400> 39
guucaauaaa gcugugggaa a 21
<210> 40
<211> 21
<212> RNA
<213> 人工序列
<220>
<223> 突变型miR396b-3p
<400> 40
guucaauaaa gcagagagaa a 21
<210> 41
<211> 21
<212> DNA
<213> 玉蜀黍
<400> 41
cagagctccc ttcactccaa a 21
<210> 42
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 突变型miR159h-3p
<400> 42
aagagcaccg ttcactccaa c 21
<210> 43
<211> 22
<212> DNA
<213> 玉蜀黍
<400> 43
gatgacagat agagcagtga gc 22
<210> 44
<211> 21
<212> DNA
<213> 玉蜀黍
<400> 44
tttgattgaa ccgtgccaac a 21
<210> 45
<211> 21
<212> DNA
<213> 玉蜀黍
<400> 45
tttcccacag ctttattgaa c 21
<210> 46
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> miR39b-3p突变体
<400> 46
tttctctctg ctttattgaa c 21
<210> 47
<211> 14976
<212> DNA
<213> 人工序列
<220>
<223> 载体24366
<400> 47
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta 1260
gatcggcgtt ccggtccata gttagggccc ggtagttcta cttctgttca tgtttgtgtt 1320
agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg 1380
tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct 1440
ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt 1500
ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt 1560
catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg 1620
gagtagaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt 1680
gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag 1740
gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg 1800
ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact 1860
gtttcaaact acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt 1920
catagttacg agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg 1980
tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc 2040
ttgagtacct atctattata ataaacaagt atgttttata attattttga tcttgatata 2100
cttggatgat ggcatatcca gcagctatat gtggattttt ttagccctgc cttcatacgc 2160
tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt 2220
ctgcaggatc ctctaagtag cagccatgga caacaacccc aacatcaacg agtgcatccc 2280
ctacaactgc ctgagcaacc ccgaggtgga ggtgctgggc ggcgagcgca tcgagaccgg 2340
ctacaccccc atcgacatca gcctgagcct gacccagttc ctgctgagcg agttcgtgcc 2400
cggcgccggc ttcgtgctgg gcctggtgga catcatctgg ggcatcttcg gccccagcca 2460
gtgggacgcc ttcctggtgc agatcgagca gttgataaac caacgcatag aggaattcgc 2520
ccgcaaccag gccatcagcc gcctggaggg cctgagcaac ctgtaccaaa tctacgccga 2580
gagcttccgc gagtgggagg ccgaccccac caaccccgcc ctgcgcgagg agatgcgcat 2640
ccagttcaac gacatgaaca gcgccctgac caccgccatc cccctgttcg ccgtgcagaa 2700
ctaccaggtg cccctgctga gcgtgtacgt gcaggccgcc aacctgcacc tgagcgtgct 2760
gcgcgacgtc agcgtgttcg gccagcgctg gggcttcgac gccgccacca tcaacagccg 2820
ctacaacgac ctgacccgcc tgatcggcaa ctacaccgac cacgccgtgc gctggtacaa 2880
caccggcctg gagcgcgtgt gggggcccga cagccgcgac tggatcaggt acaaccagtt 2940
ccgccgcgag ctgaccctga ccgtgctgga catcgtgagc ctgttcccca actacgacag 3000
ccgcacctac cccatccgca ccgtgagcca gctgacccgc gagatttaca ccaaccccgt 3060
gctggagaac ttcgacggca gcttccgcgg cagcgcccag ggcatcgagg gcagcatccg 3120
cagcccccac ctgatggaca tcctgaacag catcaccatc tacaccgacg cccaccgcgg 3180
cgagtactac tggagcggcc accagatcat ggccagcccc gtcggcttca gcggccccga 3240
gttcaccttc cccctgtacg gcacgatggg caacgctgca cctcagcagc gcatcgtggc 3300
acagctgggc cagggagtgt accgcaccct gagcagcacc ctgtaccgtc gacctttcaa 3360
catcggcatc aacaaccagc agctgagcgt gctggacggc accgagttcg cctacggcac 3420
cagcagcaac ctgcccagcg ccgtgtaccg caagagcggc accgtggaca gcctggacga 3480
gatcccccct cagaacaaca acgtgccacc tcgacagggc ttcagccacc gtctgagcca 3540
cgtgagcatg ttccgcagtg gcttcagcaa cagcagcgtg agcatcatcc gtgcacctat 3600
gttcagctgg attcaccgca gtgccgagtt caacaacatc atccccagca gccagatcac 3660
ccagatcccc ctgaccaaga gcaccaacct gggcagcggc accagcgtgg tgaagggccc 3720
cggcttcacc ggcggcgaca tcctgcgccg caccagcccc ggccagatca gcaccctgcg 3780
cgtgaacatc accgcccccc tgagccagcg ctaccgcgtc cgcatccgct acgccagcac 3840
caccaacctg cagttccaca ccagcatcga cggccgcccc atcaaccagg gcaacttcag 3900
cgccaccatg agcagcggca gcaacctgca gagcggcagc ttccgcaccg tgggcttcac 3960
cacccccttc aacttcagca acggcagcag cgtgttcacc ctgagcgccc acgtgttcaa 4020
cagcggcaac gaggtgtaca tcgaccgcat cgagttcgtg cccgccgagg tgaccttcga 4080
ggccgagtac gacctggaga gggctcagaa ggccgtgaac gagctgttca ccagcagcaa 4140
ccagatcggc ctgaagaccg acgtgaccga ctaccacatc gaccaggtga gcaacctggt 4200
ggagtgctta agcgacgagt tctgcctgga cgagaagaag gagctgagcg agaaggtgaa 4260
gcacgccaag cgcctgagcg acgagcgcaa cctgctgcag gaccccaact tccgcggcat 4320
caaccgccag ctggaccgcg gctggcgagg cagcaccgat atcaccatcc agggcggcga 4380
cgacgtgttc aaggagaact acgtgaccct gctgggcacc ttcgacgagt gctaccccac 4440
ctacctgtac cagaagatcg acgagagcaa gctgaaggcc tacacccgct accagctgcg 4500
cggctacatc gaggacagcc aggacctgga aatctacctg atccgctaca acgcgaagca 4560
cgagaccgtg aacgtgcccg gcaccggcag cctgtggccc ctgagcgccc ccagccccat 4620
cggcaagtgc actagtcacc acagccacca cttcagcctg gacatcgacg tgggctgcac 4680
cgacctgaac gaggacctgg gcgtgtgggt gatcttcaag atcaagaccc aggacggcca 4740
cgcccgcctg ggcaatctag agttcctgga ggagaagccc ctggtgggcg aggccctggc 4800
ccgcgtgaag cgtgctgaga agaagtggcg cgacaagcgc gagaagctgg agtgggagac 4860
caacatcgtg tacaaggagg ccaaggagag cgtggacgcc ctgttcgtga acagccagta 4920
cgaccgcctg caggccgaca ccaacatcgc catgatccac gccgccgaca agcgcgtgca 4980
cagcattcgc gaggcctacc tgcccgagct gagcgtgatc cccggtgtga acgccgccat 5040
cttcgaggaa ctcgagggcc gcatctagga gctctatgat cgttcaaaca tttggcaata 5100
aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt 5160
gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt 5220
ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg 5280
cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc aatagactac 5340
ggaccgcgat cgcttaatta agcttgcatg cctgcagtgc agcgtgaccc ggtcgtgccc 5400
ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt 5460
tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa actttactct 5520
acgaataata taatctatag tactacaata atatcagtgt tttagagaat catataaatg 5580
aacagttaga catggtctaa aggacaattg agtattttga caacaggact ctacagtttt 5640
atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac 5700
ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa 5760
tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct 5820
attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa gtgactaaaa 5880
attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag 5940
tagataatgc cagcctgtta aacgccgccg acgagtctaa cggacaccaa ccagcgaacc 6000
agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg 6060
acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt 6120
gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc 6180
accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc 6240
gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt tcggagcgca 6300
cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt caaggtacgc 6360
cgctcgtcct cccccccccc ccctctctac cttctctaga tcggcgttcc ggtccatagt 6420
tagggcccgg tagttctact tctgttcatg tttgtgttag atccgtgttt gtgttagatc 6480
cgtgctgtta gcgttcgtac acggatgcga cctgtacgtc agacacgttc tgattgctaa 6540
cttgccagtg tttctctttg gggaatcctg ggatggctct agccgttccg cagacgggat 6600
cgatttcatg attttttttg tttcgttgca tagggtttgg tttgcccttt tcctttattt 6660
caatatatgc cgtgcacttg tttgtcgggt catcttttca tgcttttttt tgtcttggtt 6720
gtgatgatgt ggtctggttg ggcggtcgtt ctagatcgga gtagaattct gtttcaaact 6780
acctggtgga tttattaatt ttggatctgt atgtgtgtgc catacatatt catagttacg 6840
aattgaagat gatggatgga aatatcgatc taggataggt atacatgttg atgcgggttt 6900
tactgatgca tatacagaga tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg 6960
ggcggtcgtt cattcgttct agatcggagt agaatactgt ttcaaactac ctggtgtatt 7020
tattaatttt ggaactgtat gtgtgtgtca tacatcttca tagttacgag tttaagatgg 7080
atggaaatat cgatctagga taggtataca tgttgatgtg ggttttactg atgcatatac 7140
atgatggcat atgcagcatc tattcatatg ctctaacctt gagtacctat ctattataat 7200
aaacaagtat gttttataat tattttgatc ttgatatact tggatgatgg catatgcagc 7260
agctatatgt ggattttttt agccctgcct tcatacgcta tttatttgct tggtactgtt 7320
tcttttgtcg atgctcaccc tgttgtttgg tgttacttct gcagggatct ccgatcatgc 7380
aaaaactcat taactcagtg caaaactatg cctggggcag caaaacggcg ttgactgaac 7440
tttatggtat ggaaaatccg tccagccagc cgatggccga gctgtggatg ggcgcacatc 7500
cgaaaagcag ttcacgagtg cagaatgccg ccggagatat cgtttcactg cgtgatgtga 7560
ttgagagtga taaatcgact ctgctcggag aggccgttgc caaacgcttt ggcgaactgc 7620
ctttcctgtt caaagtatta tgcgcagcac agccactctc cattcaggtt catccaaaca 7680
aacacaattc tgaaatcggt tttgccaaag aaaatgccgc aggtatcccg atggatgccg 7740
ccgagcgtaa ctataaagat cctaaccaca agccggagct ggtttttgcg ctgacgcctt 7800
tccttgcgat gaacgcgttt cgtgaatttt ccgagattgt ctccctactc cagccggtcg 7860
caggtgcaca tccggcgatt gctcactttt tacaacagcc tgatgccgaa cgtttaagcg 7920
aactgttcgc cagcctgttg aatatgcagg gtgaagaaaa atcccgcgcg ctggcgattt 7980
taaaatcggc cctcgatagc cagcagggtg aaccgtggca aacgattcgt ttaatttctg 8040
aattttaccc ggaagacagc ggtctgttct ccccgctatt gctgaatgtg gtgaaattga 8100
accctggcga agcgatgttc ctgttcgctg aaacaccgca cgcttacctg caaggcgtgg 8160
cgctggaagt gatggcaaac tccgataacg tgctgcgtgc gggtctgacg cctaaataca 8220
ttgatattcc ggaactggtt gccaatgtga aattcgaagc caaaccggct aaccagttgt 8280
tgacccagcc ggtgaaacaa ggtgcagaac tggacttccc gattccagtg gatgattttg 8340
ccttctcgct gcatgacctt agtgataaag aaaccaccat tagccagcag agtgccgcca 8400
ttttgttctg cgtcgaaggc gatgcaacgt tgtggaaagg ttctcagcag ttacagctta 8460
aaccgggtga atcagcgttt attgccgcca acgaatcacc ggtgactgtc aaaggccacg 8520
gccgtttagc gcgtgtttac aacaagctgt aagagcttac tgaaaaaatt aacatctctt 8580
gctaagctgg gtcatgggtc gtttaagctg ccgatgtgcc tgcgtcgtct ggtgccctct 8640
ctccatatgg aggttgtcaa agtatctgct gttcgtgtca tgagtcgtgt cagtgttggt 8700
ttaataatgg accggttgtg ttgtgtgtgc gtactaccca gaactatgac aaatcatgaa 8760
taagtttgat gtttgaaatt aaagcctgtg ctcattatgt tctgtctttc agttgtctcc 8820
taatatttgc ctccaggtac tggctatcta ccgtttctta cttaggaggt gtttgaatgc 8880
actaaaacta atagttagtg gctaaaatta gttaaaacat ccaaacacca tagctaatag 8940
ttgaactatt agctattttt ggaaaattag ttaatagtga ggtagttatt tgttagctag 9000
ctaattcaac taacaatttt tagccaacta acaattagtt tcagtgcatt caaacacccc 9060
cttaatgtta acgtggttct atctaccgtc tcctaatata tggttgattg ttcggtttgt 9120
tgctatgcta ttgggttctg attgctgcta gttcttgctg aatccagaag ttctcgtagt 9180
atagctcaga ttcatattat ttatttgagt gataagtgat ccaggttatt actatgttag 9240
ctaggttttt tttacaagga taaattatct gtgatcataa ttcttatgaa agctttatgt 9300
ttcctggagg cagtggcatg caatgcatga cagcaacttg atcacaccag ctgaggtaga 9360
tacggtaaca aggttcttaa atctgttcac caaatcattg gagaacacac atacacattc 9420
ttgccagtct tggttagaga aatttcatga caaaatgcca aagctgtctt gactcttcac 9480
ttttggccat gagtcgtgac ttagtttggt ttaatggacc ggttctccta gcttgttcta 9540
ctcaaaactg ttgttgatgc gaataagttg tgatggttga tctctggatt ttgttttgct 9600
ctcaatagtg gacgagatta gatagcctgc aggcccgggg gcgcgcccta attagctaac 9660
ggccaggatc gccgcgtgag cctttagcaa ctagctagat taattaacgc aatctgttat 9720
taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac 9780
agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagaat 9840
taattctcat gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca 9900
ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt 9960
cgctcaaggc gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg 10020
gcaaatattc tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat 10080
tgtgagcgga taacaatttc acacaggaaa cagaccatga gggaagcgtt gatcgccgaa 10140
gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg 10200
ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt 10260
gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac 10320
gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc 10380
accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa 10440
tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac 10500
attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca 10560
gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa 10620
accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt 10680
acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct 10740
gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctagg 10800
caggcttatc ttggacaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt 10860
gttcactacg tgaaaggcga gatcaccaaa gtagtcggca aataaagctc tagtggatct 10920
ccgtacccag ggatctggct cgcggcggac gcacgacgcc ggggcgagac cataggcgat 10980
ctcctaaatc aatagtagct gtaacctcga agcgtttcac ttgtaacaac gattgagaat 11040
ttttgtcata aaattgaaat acttggttcg catttttgtc atccgcggtc agccgcaatt 11100
ctgacgaact gcccatttag ctggagatga ttgtacatcc ttcacgtgaa aatttctcaa 11160
gcgctgtgaa caagggttca gattttagat tgaaaggtga gccgttgaaa cacgttcttc 11220
ttgtcgatga cgacgtcgct atgcggcatc ttattattga ataccttacg atccacgcct 11280
tcaaagtgac cgcggtagcc gacagcaccc agttcacaag agtactctct tccgcgacgg 11340
tcgatgtcgt ggttgttgat ctagatttag gtcgtgaaga tgggctcgag atcgttcgta 11400
atctggcggc aaagtctgat attccaatca taattatcag tggcgaccgc cttgaggaga 11460
cggataaagt tgttgcactc gagctaggag caagtgattt tatcgctaag ccgttcagta 11520
tcagagagtt tctagcacgc attcgggttg ccttgcgcgt gcgccccaac gttgtccgct 11580
ccaaagaccg acggtctttt tgttttactg actggacact taatctcagg caacgtcgct 11640
tgatgtccga agctggcggt gaggtgaaac ttacggcagg tgagttcaat cttctcctcg 11700
cgtttttaga gaaaccccgc gacgttctat cgcgcgagca acttctcatt gccagtcgag 11760
tacgcgacga ggaggtttat gacaggagta tagatgttct cattttgagg ctgcgccgca 11820
aacttgaggc agatccgtca agccctcaac tgataaaaac agcaagaggt gccggttatt 11880
tctttgacgc ggacgtgcag gtttcgcacg gggggacgat ggcagcctga gccaattccc 11940
agatccccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc 12000
ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg 12060
catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa 12120
agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga 12180
cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag 12240
catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat 12300
ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag 12360
tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg 12420
ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt 12480
actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg 12540
cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg 12600
cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga 12660
aaccgggcgg ccggagtaca tcgagatcga gctggctgat tggatgtacc gcgagatcac 12720
agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg 12780
catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg 12840
gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt 12900
caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc 12960
ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc 13020
cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg 13080
tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg 13140
gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta 13200
agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat 13260
taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga 13320
gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg 13380
gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg 13440
gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc tgaggtctgc ctcgtgaaga 13500
aggtgttgct gactcatacc aggcctgaat cgccccatca tccagccaga aagtgaggga 13560
gccacggttg atgagagctt tgttgtaggt ggaccagttg gtgattttga acttttgctt 13620
tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca actcagcaaa 13680
agttcgattt attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct ctgccagtgt 13740
tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat 13800
ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga 13860
gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg 13920
actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt 13980
gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctc tgcattaatg 14040
aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 14100
cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 14160
ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 14220
ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 14280
cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 14340
actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 14400
cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 14460
tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 14520
gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 14580
caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 14640
agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 14700
tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 14760
tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 14820
gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 14880
gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 14940
aaggatcttc acctagatcc ttttgatccg gaatta 14976
<210> 48
<211> 15033
<212> DNA
<213> 人工序列
<220>
<223> 载体24373
<400> 48
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta 1260
gatcggcgtt ccggtccata gttagggccc ggtagttcta cttctgttca tgtttgtgtt 1320
agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg 1380
tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct 1440
ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt 1500
ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt 1560
catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg 1620
gagtagaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt 1680
gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag 1740
gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg 1800
ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact 1860
gtttcaaact acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt 1920
catagttacg agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg 1980
tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc 2040
ttgagtacct atctattata ataaacaagt atgttttata attattttga tcttgatata 2100
cttggatgat ggcatatcca gcagctatat gtggattttt ttagccctgc cttcatacgc 2160
tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt 2220
ctgcaggatc ctctaagtag cagccatgga caacaacccc aacatcaacg agtgcatccc 2280
ctacaactgc ctgagcaacc ccgaggtgga ggtgctgggc ggcgagcgca tcgagaccgg 2340
ctacaccccc atcgacatca gcctgagcct gacccagttc ctgctgagcg agttcgtgcc 2400
cggcgccggc ttcgtgctgg gcctggtgga catcatctgg ggcatcttcg gccccagcca 2460
gtgggacgcc ttcctggtgc agatcgagca gttgataaac caacgcatag aggaattcgc 2520
ccgcaaccag gccatcagcc gcctggaggg cctgagcaac ctgtaccaaa tctacgccga 2580
gagcttccgc gagtgggagg ccgaccccac caaccccgcc ctgcgcgagg agatgcgcat 2640
ccagttcaac gacatgaaca gcgccctgac caccgccatc cccctgttcg ccgtgcagaa 2700
ctaccaggtg cccctgctga gcgtgtacgt gcaggccgcc aacctgcacc tgagcgtgct 2760
gcgcgacgtc agcgtgttcg gccagcgctg gggcttcgac gccgccacca tcaacagccg 2820
ctacaacgac ctgacccgcc tgatcggcaa ctacaccgac cacgccgtgc gctggtacaa 2880
caccggcctg gagcgcgtgt gggggcccga cagccgcgac tggatcaggt acaaccagtt 2940
ccgccgcgag ctgaccctga ccgtgctgga catcgtgagc ctgttcccca actacgacag 3000
ccgcacctac cccatccgca ccgtgagcca gctgacccgc gagatttaca ccaaccccgt 3060
gctggagaac ttcgacggca gcttccgcgg cagcgcccag ggcatcgagg gcagcatccg 3120
cagcccccac ctgatggaca tcctgaacag catcaccatc tacaccgacg cccaccgcgg 3180
cgagtactac tggagcggcc accagatcat ggccagcccc gtcggcttca gcggccccga 3240
gttcaccttc cccctgtacg gcacgatggg caacgctgca cctcagcagc gcatcgtggc 3300
acagctgggc cagggagtgt accgcaccct gagcagcacc ctgtaccgtc gacctttcaa 3360
catcggcatc aacaaccagc agctgagcgt gctggacggc accgagttcg cctacggcac 3420
cagcagcaac ctgcccagcg ccgtgtaccg caagagcggc accgtggaca gcctggacga 3480
gatcccccct cagaacaaca acgtgccacc tcgacagggc ttcagccacc gtctgagcca 3540
cgtgagcatg ttccgcagtg gcttcagcaa cagcagcgtg agcatcatcc gtgcacctat 3600
gttcagctgg attcaccgca gtgccgagtt caacaacatc atccccagca gccagatcac 3660
ccagatcccc ctgaccaaga gcaccaacct gggcagcggc accagcgtgg tgaagggccc 3720
cggcttcacc ggcggcgaca tcctgcgccg caccagcccc ggccagatca gcaccctgcg 3780
cgtgaacatc accgcccccc tgagccagcg ctaccgcgtc cgcatccgct acgccagcac 3840
caccaacctg cagttccaca ccagcatcga cggccgcccc atcaaccagg gcaacttcag 3900
cgccaccatg agcagcggca gcaacctgca gagcggcagc ttccgcaccg tgggcttcac 3960
cacccccttc aacttcagca acggcagcag cgtgttcacc ctgagcgccc acgtgttcaa 4020
cagcggcaac gaggtgtaca tcgaccgcat cgagttcgtg cccgccgagg tgaccttcga 4080
ggccgagtac gacctggaga gggctcagaa ggccgtgaac gagctgttca ccagcagcaa 4140
ccagatcggc ctgaagaccg acgtgaccga ctaccacatc gaccaggtga gcaacctggt 4200
ggagtgctta agcgacgagt tctgcctgga cgagaagaag gagctgagcg agaaggtgaa 4260
gcacgccaag cgcctgagcg acgagcgcaa cctgctgcag gaccccaact tccgcggcat 4320
caaccgccag ctggaccgcg gctggcgagg cagcaccgat atcaccatcc agggcggcga 4380
cgacgtgttc aaggagaact acgtgaccct gctgggcacc ttcgacgagt gctaccccac 4440
ctacctgtac cagaagatcg acgagagcaa gctgaaggcc tacacccgct accagctgcg 4500
cggctacatc gaggacagcc aggacctgga aatctacctg atccgctaca acgcgaagca 4560
cgagaccgtg aacgtgcccg gcaccggcag cctgtggccc ctgagcgccc ccagccccat 4620
cggcaagtgc actagcatcc ttttcattga taagaagagc accgttcact ccaactgcaa 4680
acttgtggct agtcaccaca gccaccactt cagcctggac atcgacgtgg gctgcaccga 4740
cctgaacgag gacctgggcg tgtgggtgat cttcaagatc aagacccagg acggccacgc 4800
ccgcctgggc aatctagagt tcctggagga gaagcccctg gtgggcgagg ccctggcccg 4860
cgtgaagcgt gctgagaaga agtggcgcga caagcgcgag aagctggagt gggagaccaa 4920
catcgtgtac aaggaggcca aggagagcgt ggacgccctg ttcgtgaaca gccagtacga 4980
ccgcctgcag gccgacacca acatcgccat gatccacgcc gccgacaagc gcgtgcacag 5040
cattcgcgag gcctacctgc ccgagctgag cgtgatcccc ggtgtgaacg ccgccatctt 5100
cgaggaactc gagggccgca tctaggagct ctatgatcgt tcaaacattt ggcaataaag 5160
tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa 5220
ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt 5280
tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc 5340
aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcaat agactacgga 5400
ccgcgatcgc ttaattaagc ttgcatgcct gcagtgcagc gtgacccggt cgtgcccctc 5460
tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt 5520
cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg 5580
aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac 5640
agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc 5700
tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc 5760
atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt 5820
ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt 5880
ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt 5940
aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag 6000
ataatgccag cctgttaaac gccgccgacg agtctaacgg acaccaacca gcgaaccagc 6060
agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc 6120
cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg 6180
tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacc 6240
ggcagctacg ggggattcct ttcccaccgc tccttcgctt tcccttcctc gcccgccgta 6300
ataaatagac accccctcca caccctcttt ccccaacctc gtgttgttcg gagcgcacac 6360
acacacaacc agatctcccc caaatccacc cgtcggcacc tccgcttcaa ggtacgccgc 6420
tcgtcctccc cccccccccc tctctacctt ctctagatcg gcgttccggt ccatagttag 6480
ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt 6540
gctgttagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga ttgctaactt 6600
gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag acgggatcga 6660
tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc tttatttcaa 6720
tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt cttggttgtg 6780
atgatgtggt ctggttgggc ggtcgttcta gatcggagta gaattctgtt tcaaactacc 6840
tggtggattt attaattttg gatctgtatg tgtgtgccat acatattcat agttacgaat 6900
tgaagatgat ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac 6960
tgatgcatat acagagatgc tttttgttcg cttggttgtg atgatgtggt gtggttgggc 7020
ggtcgttcat tcgttctaga tcggagtaga atactgtttc aaactacctg gtgtatttat 7080
taattttgga actgtatgtg tgtgtcatac atcttcatag ttacgagttt aagatggatg 7140
gaaatatcga tctaggatag gtatacatgt tgatgtgggt tttactgatg catatacatg 7200
atggcatatg cagcatctat tcatatgctc taaccttgag tacctatcta ttataataaa 7260
caagtatgtt ttataattat tttgatcttg atatacttgg atgatggcat atgcagcagc 7320
tatatgtgga tttttttagc cctgccttca tacgctattt atttgcttgg tactgtttct 7380
tttgtcgatg ctcaccctgt tgtttggtgt tacttctgca gggatctccg atcatgcaaa 7440
aactcattaa ctcagtgcaa aactatgcct ggggcagcaa aacggcgttg actgaacttt 7500
atggtatgga aaatccgtcc agccagccga tggccgagct gtggatgggc gcacatccga 7560
aaagcagttc acgagtgcag aatgccgccg gagatatcgt ttcactgcgt gatgtgattg 7620
agagtgataa atcgactctg ctcggagagg ccgttgccaa acgctttggc gaactgcctt 7680
tcctgttcaa agtattatgc gcagcacagc cactctccat tcaggttcat ccaaacaaac 7740
acaattctga aatcggtttt gccaaagaaa atgccgcagg tatcccgatg gatgccgccg 7800
agcgtaacta taaagatcct aaccacaagc cggagctggt ttttgcgctg acgcctttcc 7860
ttgcgatgaa cgcgtttcgt gaattttccg agattgtctc cctactccag ccggtcgcag 7920
gtgcacatcc ggcgattgct cactttttac aacagcctga tgccgaacgt ttaagcgaac 7980
tgttcgccag cctgttgaat atgcagggtg aagaaaaatc ccgcgcgctg gcgattttaa 8040
aatcggccct cgatagccag cagggtgaac cgtggcaaac gattcgttta atttctgaat 8100
tttacccgga agacagcggt ctgttctccc cgctattgct gaatgtggtg aaattgaacc 8160
ctggcgaagc gatgttcctg ttcgctgaaa caccgcacgc ttacctgcaa ggcgtggcgc 8220
tggaagtgat ggcaaactcc gataacgtgc tgcgtgcggg tctgacgcct aaatacattg 8280
atattccgga actggttgcc aatgtgaaat tcgaagccaa accggctaac cagttgttga 8340
cccagccggt gaaacaaggt gcagaactgg acttcccgat tccagtggat gattttgcct 8400
tctcgctgca tgaccttagt gataaagaaa ccaccattag ccagcagagt gccgccattt 8460
tgttctgcgt cgaaggcgat gcaacgttgt ggaaaggttc tcagcagtta cagcttaaac 8520
cgggtgaatc agcgtttatt gccgccaacg aatcaccggt gactgtcaaa ggccacggcc 8580
gtttagcgcg tgtttacaac aagctgtaag agcttactga aaaaattaac atctcttgct 8640
aagctgggtc atgggtcgtt taagctgccg atgtgcctgc gtcgtctggt gccctctctc 8700
catatggagg ttgtcaaagt atctgctgtt cgtgtcatga gtcgtgtcag tgttggttta 8760
ataatggacc ggttgtgttg tgtgtgcgta ctacccagaa ctatgacaaa tcatgaataa 8820
gtttgatgtt tgaaattaaa gcctgtgctc attatgttct gtctttcagt tgtctcctaa 8880
tatttgcctc caggtactgg ctatctaccg tttcttactt aggaggtgtt tgaatgcact 8940
aaaactaata gttagtggct aaaattagtt aaaacatcca aacaccatag ctaatagttg 9000
aactattagc tatttttgga aaattagtta atagtgaggt agttatttgt tagctagcta 9060
attcaactaa caatttttag ccaactaaca attagtttca gtgcattcaa acaccccctt 9120
aatgttaacg tggttctatc taccgtctcc taatatatgg ttgattgttc ggtttgttgc 9180
tatgctattg ggttctgatt gctgctagtt cttgctgaat ccagaagttc tcgtagtata 9240
gctcagattc atattattta tttgagtgat aagtgatcca ggttattact atgttagcta 9300
ggtttttttt acaaggataa attatctgtg atcataattc ttatgaaagc tttatgtttc 9360
ctggaggcag tggcatgcaa tgcatgacag caacttgatc acaccagctg aggtagatac 9420
ggtaacaagg ttcttaaatc tgttcaccaa atcattggag aacacacata cacattcttg 9480
ccagtcttgg ttagagaaat ttcatgacaa aatgccaaag ctgtcttgac tcttcacttt 9540
tggccatgag tcgtgactta gtttggttta atggaccggt tctcctagct tgttctactc 9600
aaaactgttg ttgatgcgaa taagttgtga tggttgatct ctggattttg ttttgctctc 9660
aatagtggac gagattagat agcctgcagg cccgggggcg cgccctaatt agctaacggc 9720
caggatcgcc gcgtgagcct ttagcaacta gctagattaa ttaacgcaat ctgttattaa 9780
gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc 9840
tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca tcagaattaa 9900
ttctcatgtt tgacagctta tcatcgactg cacggtgcac caatgcttct ggcgtcaggc 9960
agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc 10020
tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca 10080
aatattctga aatgagctgt tgacaattaa tcatccggct cgtataatgt gtggaattgt 10140
gagcggataa caatttcaca caggaaacag accatgaggg aagcgttgat cgccgaagta 10200
tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg 10260
gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat 10320
ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac 10380
cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc 10440
attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt 10500
ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt 10560
gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg 10620
gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc 10680
ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg 10740
ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc 10800
gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctaggcag 10860
gcttatcttg gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt 10920
cactacgtga aaggcgagat caccaaagta gtcggcaaat aaagctctag tggatctccg 10980
tacccaggga tctggctcgc ggcggacgca cgacgccggg gcgagaccat aggcgatctc 11040
ctaaatcaat agtagctgta acctcgaagc gtttcacttg taacaacgat tgagaatttt 11100
tgtcataaaa ttgaaatact tggttcgcat ttttgtcatc cgcggtcagc cgcaattctg 11160
acgaactgcc catttagctg gagatgattg tacatccttc acgtgaaaat ttctcaagcg 11220
ctgtgaacaa gggttcagat tttagattga aaggtgagcc gttgaaacac gttcttcttg 11280
tcgatgacga cgtcgctatg cggcatctta ttattgaata ccttacgatc cacgccttca 11340
aagtgaccgc ggtagccgac agcacccagt tcacaagagt actctcttcc gcgacggtcg 11400
atgtcgtggt tgttgatcta gatttaggtc gtgaagatgg gctcgagatc gttcgtaatc 11460
tggcggcaaa gtctgatatt ccaatcataa ttatcagtgg cgaccgcctt gaggagacgg 11520
ataaagttgt tgcactcgag ctaggagcaa gtgattttat cgctaagccg ttcagtatca 11580
gagagtttct agcacgcatt cgggttgcct tgcgcgtgcg ccccaacgtt gtccgctcca 11640
aagaccgacg gtctttttgt tttactgact ggacacttaa tctcaggcaa cgtcgcttga 11700
tgtccgaagc tggcggtgag gtgaaactta cggcaggtga gttcaatctt ctcctcgcgt 11760
ttttagagaa accccgcgac gttctatcgc gcgagcaact tctcattgcc agtcgagtac 11820
gcgacgagga ggtttatgac aggagtatag atgttctcat tttgaggctg cgccgcaaac 11880
ttgaggcaga tccgtcaagc cctcaactga taaaaacagc aagaggtgcc ggttatttct 11940
ttgacgcgga cgtgcaggtt tcgcacgggg ggacgatggc agcctgagcc aattcccaga 12000
tccccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc 12060
gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat 12120
cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga 12180
atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga 12240
gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat 12300
catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg 12360
ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt 12420
gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata 12480
ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact 12540
caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat 12600
tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct 12660
ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac 12720
cgggcggccg gagtacatcg agatcgagct ggctgattgg atgtaccgcg agatcacaga 12780
aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat 12840
cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt 12900
gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac 12960
cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg 13020
gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc 13080
cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg 13140
aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa 13200
ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt 13260
gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa 13320
aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct 13380
gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc 13440
tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg 13500
cggacaagcc gcgccgtcgc cactcgaccg ccggcgctga ggtctgcctc gtgaagaagg 13560
tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag tgagggagcc 13620
acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact tttgctttgc 13680
cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact cagcaaaagt 13740
tcgatttatt caacaaagcc gccgtcccgt caagtcagcg taatgctctg ccagtgttac 13800
aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta 13860
ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa 13920
aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact 13980
cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag 14040
aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagctctgc attaatgaat 14100
cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 14160
tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 14220
aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 14280
gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 14340
ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 14400
ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 14460
gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 14520
ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 14580
cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 14640
cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 14700
gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 14760
aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 14820
tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 14880
gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 14940
tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 15000
gatcttcacc tagatccttt tgatccggaa tta 15033
<210> 49
<211> 15033
<212> DNA
<213> 人工序列
<220>
<223> 载体24374
<400> 49
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta 1260
gatcggcgtt ccggtccata gttagggccc ggtagttcta cttctgttca tgtttgtgtt 1320
agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg 1380
tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct 1440
ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt 1500
ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt 1560
catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg 1620
gagtagaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt 1680
gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag 1740
gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg 1800
ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact 1860
gtttcaaact acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt 1920
catagttacg agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg 1980
tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc 2040
ttgagtacct atctattata ataaacaagt atgttttata attattttga tcttgatata 2100
cttggatgat ggcatatcca gcagctatat gtggattttt ttagccctgc cttcatacgc 2160
tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt 2220
ctgcaggatc ctctaagtag cagccatgga caacaacccc aacatcaacg agtgcatccc 2280
ctacaactgc ctgagcaacc ccgaggtgga ggtgctgggc ggcgagcgca tcgagaccgg 2340
ctacaccccc atcgacatca gcctgagcct gacccagttc ctgctgagcg agttcgtgcc 2400
cggcgccggc ttcgtgctgg gcctggtgga catcatctgg ggcatcttcg gccccagcca 2460
gtgggacgcc ttcctggtgc agatcgagca gttgataaac caacgcatag aggaattcgc 2520
ccgcaaccag gccatcagcc gcctggaggg cctgagcaac ctgtaccaaa tctacgccga 2580
gagcttccgc gagtgggagg ccgaccccac caaccccgcc ctgcgcgagg agatgcgcat 2640
ccagttcaac gacatgaaca gcgccctgac caccgccatc cccctgttcg ccgtgcagaa 2700
ctaccaggtg cccctgctga gcgtgtacgt gcaggccgcc aacctgcacc tgagcgtgct 2760
gcgcgacgtc agcgtgttcg gccagcgctg gggcttcgac gccgccacca tcaacagccg 2820
ctacaacgac ctgacccgcc tgatcggcaa ctacaccgac cacgccgtgc gctggtacaa 2880
caccggcctg gagcgcgtgt gggggcccga cagccgcgac tggatcaggt acaaccagtt 2940
ccgccgcgag ctgaccctga ccgtgctgga catcgtgagc ctgttcccca actacgacag 3000
ccgcacctac cccatccgca ccgtgagcca gctgacccgc gagatttaca ccaaccccgt 3060
gctggagaac ttcgacggca gcttccgcgg cagcgcccag ggcatcgagg gcagcatccg 3120
cagcccccac ctgatggaca tcctgaacag catcaccatc tacaccgacg cccaccgcgg 3180
cgagtactac tggagcggcc accagatcat ggccagcccc gtcggcttca gcggccccga 3240
gttcaccttc cccctgtacg gcacgatggg caacgctgca cctcagcagc gcatcgtggc 3300
acagctgggc cagggagtgt accgcaccct gagcagcacc ctgtaccgtc gacctttcaa 3360
catcggcatc aacaaccagc agctgagcgt gctggacggc accgagttcg cctacggcac 3420
cagcagcaac ctgcccagcg ccgtgtaccg caagagcggc accgtggaca gcctggacga 3480
gatcccccct cagaacaaca acgtgccacc tcgacagggc ttcagccacc gtctgagcca 3540
cgtgagcatg ttccgcagtg gcttcagcaa cagcagcgtg agcatcatcc gtgcacctat 3600
gttcagctgg attcaccgca gtgccgagtt caacaacatc atccccagca gccagatcac 3660
ccagatcccc ctgaccaaga gcaccaacct gggcagcggc accagcgtgg tgaagggccc 3720
cggcttcacc ggcggcgaca tcctgcgccg caccagcccc ggccagatca gcaccctgcg 3780
cgtgaacatc accgcccccc tgagccagcg ctaccgcgtc cgcatccgct acgccagcac 3840
caccaacctg cagttccaca ccagcatcga cggccgcccc atcaaccagg gcaacttcag 3900
cgccaccatg agcagcggca gcaacctgca gagcggcagc ttccgcaccg tgggcttcac 3960
cacccccttc aacttcagca acggcagcag cgtgttcacc ctgagcgccc acgtgttcaa 4020
cagcggcaac gaggtgtaca tcgaccgcat cgagttcgtg cccgccgagg tgaccttcga 4080
ggccgagtac gacctggaga gggctcagaa ggccgtgaac gagctgttca ccagcagcaa 4140
ccagatcggc ctgaagaccg acgtgaccga ctaccacatc gaccaggtga gcaacctggt 4200
ggagtgctta agcgacgagt tctgcctgga cgagaagaag gagctgagcg agaaggtgaa 4260
gcacgccaag cgcctgagcg acgagcgcaa cctgctgcag gaccccaact tccgcggcat 4320
caaccgccag ctggaccgcg gctggcgagg cagcaccgat atcaccatcc agggcggcga 4380
cgacgtgttc aaggagaact acgtgaccct gctgggcacc ttcgacgagt gctaccccac 4440
ctacctgtac cagaagatcg acgagagcaa gctgaaggcc tacacccgct accagctgcg 4500
cggctacatc gaggacagcc aggacctgga aatctacctg atccgctaca acgcgaagca 4560
cgagaccgtg aacgtgcccg gcaccggcag cctgtggccc ctgagcgccc ccagccccat 4620
cggcaagtgc actagcacat ccaacacgca cgagatgaca gatagagcag tgagcagacc 4680
atctcttact agtcaccaca gccaccactt cagcctggac atcgacgtgg gctgcaccga 4740
cctgaacgag gacctgggcg tgtgggtgat cttcaagatc aagacccagg acggccacgc 4800
ccgcctgggc aatctagagt tcctggagga gaagcccctg gtgggcgagg ccctggcccg 4860
cgtgaagcgt gctgagaaga agtggcgcga caagcgcgag aagctggagt gggagaccaa 4920
catcgtgtac aaggaggcca aggagagcgt ggacgccctg ttcgtgaaca gccagtacga 4980
ccgcctgcag gccgacacca acatcgccat gatccacgcc gccgacaagc gcgtgcacag 5040
cattcgcgag gcctacctgc ccgagctgag cgtgatcccc ggtgtgaacg ccgccatctt 5100
cgaggaactc gagggccgca tctaggagct ctatgatcgt tcaaacattt ggcaataaag 5160
tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa 5220
ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt 5280
tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc 5340
aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcaat agactacgga 5400
ccgcgatcgc ttaattaagc ttgcatgcct gcagtgcagc gtgacccggt cgtgcccctc 5460
tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt 5520
cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg 5580
aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac 5640
agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc 5700
tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc 5760
atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt 5820
ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt 5880
ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt 5940
aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag 6000
ataatgccag cctgttaaac gccgccgacg agtctaacgg acaccaacca gcgaaccagc 6060
agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc 6120
cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg 6180
tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacc 6240
ggcagctacg ggggattcct ttcccaccgc tccttcgctt tcccttcctc gcccgccgta 6300
ataaatagac accccctcca caccctcttt ccccaacctc gtgttgttcg gagcgcacac 6360
acacacaacc agatctcccc caaatccacc cgtcggcacc tccgcttcaa ggtacgccgc 6420
tcgtcctccc cccccccccc tctctacctt ctctagatcg gcgttccggt ccatagttag 6480
ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt 6540
gctgttagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga ttgctaactt 6600
gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag acgggatcga 6660
tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc tttatttcaa 6720
tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt cttggttgtg 6780
atgatgtggt ctggttgggc ggtcgttcta gatcggagta gaattctgtt tcaaactacc 6840
tggtggattt attaattttg gatctgtatg tgtgtgccat acatattcat agttacgaat 6900
tgaagatgat ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac 6960
tgatgcatat acagagatgc tttttgttcg cttggttgtg atgatgtggt gtggttgggc 7020
ggtcgttcat tcgttctaga tcggagtaga atactgtttc aaactacctg gtgtatttat 7080
taattttgga actgtatgtg tgtgtcatac atcttcatag ttacgagttt aagatggatg 7140
gaaatatcga tctaggatag gtatacatgt tgatgtgggt tttactgatg catatacatg 7200
atggcatatg cagcatctat tcatatgctc taaccttgag tacctatcta ttataataaa 7260
caagtatgtt ttataattat tttgatcttg atatacttgg atgatggcat atgcagcagc 7320
tatatgtgga tttttttagc cctgccttca tacgctattt atttgcttgg tactgtttct 7380
tttgtcgatg ctcaccctgt tgtttggtgt tacttctgca gggatctccg atcatgcaaa 7440
aactcattaa ctcagtgcaa aactatgcct ggggcagcaa aacggcgttg actgaacttt 7500
atggtatgga aaatccgtcc agccagccga tggccgagct gtggatgggc gcacatccga 7560
aaagcagttc acgagtgcag aatgccgccg gagatatcgt ttcactgcgt gatgtgattg 7620
agagtgataa atcgactctg ctcggagagg ccgttgccaa acgctttggc gaactgcctt 7680
tcctgttcaa agtattatgc gcagcacagc cactctccat tcaggttcat ccaaacaaac 7740
acaattctga aatcggtttt gccaaagaaa atgccgcagg tatcccgatg gatgccgccg 7800
agcgtaacta taaagatcct aaccacaagc cggagctggt ttttgcgctg acgcctttcc 7860
ttgcgatgaa cgcgtttcgt gaattttccg agattgtctc cctactccag ccggtcgcag 7920
gtgcacatcc ggcgattgct cactttttac aacagcctga tgccgaacgt ttaagcgaac 7980
tgttcgccag cctgttgaat atgcagggtg aagaaaaatc ccgcgcgctg gcgattttaa 8040
aatcggccct cgatagccag cagggtgaac cgtggcaaac gattcgttta atttctgaat 8100
tttacccgga agacagcggt ctgttctccc cgctattgct gaatgtggtg aaattgaacc 8160
ctggcgaagc gatgttcctg ttcgctgaaa caccgcacgc ttacctgcaa ggcgtggcgc 8220
tggaagtgat ggcaaactcc gataacgtgc tgcgtgcggg tctgacgcct aaatacattg 8280
atattccgga actggttgcc aatgtgaaat tcgaagccaa accggctaac cagttgttga 8340
cccagccggt gaaacaaggt gcagaactgg acttcccgat tccagtggat gattttgcct 8400
tctcgctgca tgaccttagt gataaagaaa ccaccattag ccagcagagt gccgccattt 8460
tgttctgcgt cgaaggcgat gcaacgttgt ggaaaggttc tcagcagtta cagcttaaac 8520
cgggtgaatc agcgtttatt gccgccaacg aatcaccggt gactgtcaaa ggccacggcc 8580
gtttagcgcg tgtttacaac aagctgtaag agcttactga aaaaattaac atctcttgct 8640
aagctgggtc atgggtcgtt taagctgccg atgtgcctgc gtcgtctggt gccctctctc 8700
catatggagg ttgtcaaagt atctgctgtt cgtgtcatga gtcgtgtcag tgttggttta 8760
ataatggacc ggttgtgttg tgtgtgcgta ctacccagaa ctatgacaaa tcatgaataa 8820
gtttgatgtt tgaaattaaa gcctgtgctc attatgttct gtctttcagt tgtctcctaa 8880
tatttgcctc caggtactgg ctatctaccg tttcttactt aggaggtgtt tgaatgcact 8940
aaaactaata gttagtggct aaaattagtt aaaacatcca aacaccatag ctaatagttg 9000
aactattagc tatttttgga aaattagtta atagtgaggt agttatttgt tagctagcta 9060
attcaactaa caatttttag ccaactaaca attagtttca gtgcattcaa acaccccctt 9120
aatgttaacg tggttctatc taccgtctcc taatatatgg ttgattgttc ggtttgttgc 9180
tatgctattg ggttctgatt gctgctagtt cttgctgaat ccagaagttc tcgtagtata 9240
gctcagattc atattattta tttgagtgat aagtgatcca ggttattact atgttagcta 9300
ggtttttttt acaaggataa attatctgtg atcataattc ttatgaaagc tttatgtttc 9360
ctggaggcag tggcatgcaa tgcatgacag caacttgatc acaccagctg aggtagatac 9420
ggtaacaagg ttcttaaatc tgttcaccaa atcattggag aacacacata cacattcttg 9480
ccagtcttgg ttagagaaat ttcatgacaa aatgccaaag ctgtcttgac tcttcacttt 9540
tggccatgag tcgtgactta gtttggttta atggaccggt tctcctagct tgttctactc 9600
aaaactgttg ttgatgcgaa taagttgtga tggttgatct ctggattttg ttttgctctc 9660
aatagtggac gagattagat agcctgcagg cccgggggcg cgccctaatt agctaacggc 9720
caggatcgcc gcgtgagcct ttagcaacta gctagattaa ttaacgcaat ctgttattaa 9780
gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc 9840
tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca tcagaattaa 9900
ttctcatgtt tgacagctta tcatcgactg cacggtgcac caatgcttct ggcgtcaggc 9960
agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc 10020
tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca 10080
aatattctga aatgagctgt tgacaattaa tcatccggct cgtataatgt gtggaattgt 10140
gagcggataa caatttcaca caggaaacag accatgaggg aagcgttgat cgccgaagta 10200
tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg 10260
gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat 10320
ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac 10380
cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc 10440
attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt 10500
ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt 10560
gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg 10620
gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc 10680
ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg 10740
ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc 10800
gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctaggcag 10860
gcttatcttg gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt 10920
cactacgtga aaggcgagat caccaaagta gtcggcaaat aaagctctag tggatctccg 10980
tacccaggga tctggctcgc ggcggacgca cgacgccggg gcgagaccat aggcgatctc 11040
ctaaatcaat agtagctgta acctcgaagc gtttcacttg taacaacgat tgagaatttt 11100
tgtcataaaa ttgaaatact tggttcgcat ttttgtcatc cgcggtcagc cgcaattctg 11160
acgaactgcc catttagctg gagatgattg tacatccttc acgtgaaaat ttctcaagcg 11220
ctgtgaacaa gggttcagat tttagattga aaggtgagcc gttgaaacac gttcttcttg 11280
tcgatgacga cgtcgctatg cggcatctta ttattgaata ccttacgatc cacgccttca 11340
aagtgaccgc ggtagccgac agcacccagt tcacaagagt actctcttcc gcgacggtcg 11400
atgtcgtggt tgttgatcta gatttaggtc gtgaagatgg gctcgagatc gttcgtaatc 11460
tggcggcaaa gtctgatatt ccaatcataa ttatcagtgg cgaccgcctt gaggagacgg 11520
ataaagttgt tgcactcgag ctaggagcaa gtgattttat cgctaagccg ttcagtatca 11580
gagagtttct agcacgcatt cgggttgcct tgcgcgtgcg ccccaacgtt gtccgctcca 11640
aagaccgacg gtctttttgt tttactgact ggacacttaa tctcaggcaa cgtcgcttga 11700
tgtccgaagc tggcggtgag gtgaaactta cggcaggtga gttcaatctt ctcctcgcgt 11760
ttttagagaa accccgcgac gttctatcgc gcgagcaact tctcattgcc agtcgagtac 11820
gcgacgagga ggtttatgac aggagtatag atgttctcat tttgaggctg cgccgcaaac 11880
ttgaggcaga tccgtcaagc cctcaactga taaaaacagc aagaggtgcc ggttatttct 11940
ttgacgcgga cgtgcaggtt tcgcacgggg ggacgatggc agcctgagcc aattcccaga 12000
tccccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc 12060
gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat 12120
cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga 12180
atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga 12240
gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat 12300
catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg 12360
ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt 12420
gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata 12480
ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact 12540
caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat 12600
tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct 12660
ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac 12720
cgggcggccg gagtacatcg agatcgagct ggctgattgg atgtaccgcg agatcacaga 12780
aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat 12840
cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt 12900
gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac 12960
cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg 13020
gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc 13080
cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg 13140
aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa 13200
ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt 13260
gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa 13320
aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct 13380
gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc 13440
tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg 13500
cggacaagcc gcgccgtcgc cactcgaccg ccggcgctga ggtctgcctc gtgaagaagg 13560
tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag tgagggagcc 13620
acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact tttgctttgc 13680
cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact cagcaaaagt 13740
tcgatttatt caacaaagcc gccgtcccgt caagtcagcg taatgctctg ccagtgttac 13800
aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta 13860
ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa 13920
aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact 13980
cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag 14040
aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagctctgc attaatgaat 14100
cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 14160
tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 14220
aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 14280
gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 14340
ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 14400
ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 14460
gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 14520
ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 14580
cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 14640
cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 14700
gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 14760
aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 14820
tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 14880
gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 14940
tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 15000
gatcttcacc tagatccttt tgatccggaa tta 15033
<210> 50
<211> 15030
<212> DNA
<213> 人工序列
<220>
<223> 载体24375
<400> 50
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta 1260
gatcggcgtt ccggtccata gttagggccc ggtagttcta cttctgttca tgtttgtgtt 1320
agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg 1380
tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct 1440
ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt 1500
ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt 1560
catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg 1620
gagtagaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt 1680
gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag 1740
gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg 1800
ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact 1860
gtttcaaact acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt 1920
catagttacg agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg 1980
tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc 2040
ttgagtacct atctattata ataaacaagt atgttttata attattttga tcttgatata 2100
cttggatgat ggcatatcca gcagctatat gtggattttt ttagccctgc cttcatacgc 2160
tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt 2220
ctgcaggatc ctctaagtag cagccatgga caacaacccc aacatcaacg agtgcatccc 2280
ctacaactgc ctgagcaacc ccgaggtgga ggtgctgggc ggcgagcgca tcgagaccgg 2340
ctacaccccc atcgacatca gcctgagcct gacccagttc ctgctgagcg agttcgtgcc 2400
cggcgccggc ttcgtgctgg gcctggtgga catcatctgg ggcatcttcg gccccagcca 2460
gtgggacgcc ttcctggtgc agatcgagca gttgataaac caacgcatag aggaattcgc 2520
ccgcaaccag gccatcagcc gcctggaggg cctgagcaac ctgtaccaaa tctacgccga 2580
gagcttccgc gagtgggagg ccgaccccac caaccccgcc ctgcgcgagg agatgcgcat 2640
ccagttcaac gacatgaaca gcgccctgac caccgccatc cccctgttcg ccgtgcagaa 2700
ctaccaggtg cccctgctga gcgtgtacgt gcaggccgcc aacctgcacc tgagcgtgct 2760
gcgcgacgtc agcgtgttcg gccagcgctg gggcttcgac gccgccacca tcaacagccg 2820
ctacaacgac ctgacccgcc tgatcggcaa ctacaccgac cacgccgtgc gctggtacaa 2880
caccggcctg gagcgcgtgt gggggcccga cagccgcgac tggatcaggt acaaccagtt 2940
ccgccgcgag ctgaccctga ccgtgctgga catcgtgagc ctgttcccca actacgacag 3000
ccgcacctac cccatccgca ccgtgagcca gctgacccgc gagatttaca ccaaccccgt 3060
gctggagaac ttcgacggca gcttccgcgg cagcgcccag ggcatcgagg gcagcatccg 3120
cagcccccac ctgatggaca tcctgaacag catcaccatc tacaccgacg cccaccgcgg 3180
cgagtactac tggagcggcc accagatcat ggccagcccc gtcggcttca gcggccccga 3240
gttcaccttc cccctgtacg gcacgatggg caacgctgca cctcagcagc gcatcgtggc 3300
acagctgggc cagggagtgt accgcaccct gagcagcacc ctgtaccgtc gacctttcaa 3360
catcggcatc aacaaccagc agctgagcgt gctggacggc accgagttcg cctacggcac 3420
cagcagcaac ctgcccagcg ccgtgtaccg caagagcggc accgtggaca gcctggacga 3480
gatcccccct cagaacaaca acgtgccacc tcgacagggc ttcagccacc gtctgagcca 3540
cgtgagcatg ttccgcagtg gcttcagcaa cagcagcgtg agcatcatcc gtgcacctat 3600
gttcagctgg attcaccgca gtgccgagtt caacaacatc atccccagca gccagatcac 3660
ccagatcccc ctgaccaaga gcaccaacct gggcagcggc accagcgtgg tgaagggccc 3720
cggcttcacc ggcggcgaca tcctgcgccg caccagcccc ggccagatca gcaccctgcg 3780
cgtgaacatc accgcccccc tgagccagcg ctaccgcgtc cgcatccgct acgccagcac 3840
caccaacctg cagttccaca ccagcatcga cggccgcccc atcaaccagg gcaacttcag 3900
cgccaccatg agcagcggca gcaacctgca gagcggcagc ttccgcaccg tgggcttcac 3960
cacccccttc aacttcagca acggcagcag cgtgttcacc ctgagcgccc acgtgttcaa 4020
cagcggcaac gaggtgtaca tcgaccgcat cgagttcgtg cccgccgagg tgaccttcga 4080
ggccgagtac gacctggaga gggctcagaa ggccgtgaac gagctgttca ccagcagcaa 4140
ccagatcggc ctgaagaccg acgtgaccga ctaccacatc gaccaggtga gcaacctggt 4200
ggagtgctta agcgacgagt tctgcctgga cgagaagaag gagctgagcg agaaggtgaa 4260
gcacgccaag cgcctgagcg acgagcgcaa cctgctgcag gaccccaact tccgcggcat 4320
caaccgccag ctggaccgcg gctggcgagg cagcaccgat atcaccatcc agggcggcga 4380
cgacgtgttc aaggagaact acgtgaccct gctgggcacc ttcgacgagt gctaccccac 4440
ctacctgtac cagaagatcg acgagagcaa gctgaaggcc tacacccgct accagctgcg 4500
cggctacatc gaggacagcc aggacctgga aatctacctg atccgctaca acgcgaagca 4560
cgagaccgtg aacgtgcccg gcaccggcag cctgtggccc ctgagcgccc ccagccccat 4620
cggcaagtgc actagcatct ctgttgcctt tttgattgaa ccgtgccaac agcttgttgg 4680
atttactagt caccacagcc accacttcag cctggacatc gacgtgggct gcaccgacct 4740
gaacgaggac ctgggcgtgt gggtgatctt caagatcaag acccaggacg gccacgcccg 4800
cctgggcaat ctagagttcc tggaggagaa gcccctggtg ggcgaggccc tggcccgcgt 4860
gaagcgtgct gagaagaagt ggcgcgacaa gcgcgagaag ctggagtggg agaccaacat 4920
cgtgtacaag gaggccaagg agagcgtgga cgccctgttc gtgaacagcc agtacgaccg 4980
cctgcaggcc gacaccaaca tcgccatgat ccacgccgcc gacaagcgcg tgcacagcat 5040
tcgcgaggcc tacctgcccg agctgagcgt gatccccggt gtgaacgccg ccatcttcga 5100
ggaactcgag ggccgcatct aggagctcta tgatcgttca aacatttggc aataaagttt 5160
cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta 5220
cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat 5280
gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa 5340
ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcaataga ctacggaccg 5400
cgatcgctta attaagcttg catgcctgca gtgcagcgtg acccggtcgt gcccctctct 5460
agagataatg agcattgcat gtctaagtta taaaaaatta ccacatattt tttttgtcac 5520
acttgtttga agtgcagttt atctatcttt atacatatat ttaaacttta ctctacgaat 5580
aatataatct atagtactac aataatatca gtgttttaga gaatcatata aatgaacagt 5640
tagacatggt ctaaaggaca attgagtatt ttgacaacag gactctacag ttttatcttt 5700
ttagtgtgca tgtgttctcc tttttttttg caaatagctt cacctatata atacttcatc 5760
cattttatta gtacatccat ttagggttta gggttaatgg tttttataga ctaatttttt 5820
tagtacatct attttattct attttagcct ctaaattaag aaaactaaaa ctctatttta 5880
gtttttttat ttaataattt agatataaaa tagaataaaa taaagtgact aaaaattaaa 5940
caaataccct ttaagaaatt aaaaaaacta aggaaacatt tttcttgttt cgagtagata 6000
atgccagcct gttaaacgcc gccgacgagt ctaacggaca ccaaccagcg aaccagcagc 6060
gtcgcgtcgg gccaagcgaa gcagacggca cggcatctct gtcgctgcct ctggacccct 6120
ctcgagagtt ccgctccacc gttggacttg ctccgctgtc ggcatccaga aattgcgtgg 6180
cggagcggca gacgtgagcc ggcacggcag gcggcctcct cctcctctca cggcaccggc 6240
agctacgggg gattcctttc ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata 6300
aatagacacc ccctccacac cctctttccc caacctcgtg ttgttcggag cgcacacaca 6360
cacaaccaga tctcccccaa atccacccgt cggcacctcc gcttcaaggt acgccgctcg 6420
tcctcccccc ccccccctct ctaccttctc tagatcggcg ttccggtcca tagttagggc 6480
ccggtagttc tacttctgtt catgtttgtg ttagatccgt gtttgtgtta gatccgtgct 6540
gttagcgttc gtacacggat gcgacctgta cgtcagacac gttctgattg ctaacttgcc 6600
agtgtttctc tttggggaat cctgggatgg ctctagccgt tccgcagacg ggatcgattt 6660
catgattttt tttgtttcgt tgcatagggt ttggtttgcc cttttccttt atttcaatat 6720
atgccgtgca cttgtttgtc gggtcatctt ttcatgcttt tttttgtctt ggttgtgatg 6780
atgtggtctg gttgggcggt cgttctagat cggagtagaa ttctgtttca aactacctgg 6840
tggatttatt aattttggat ctgtatgtgt gtgccataca tattcatagt tacgaattga 6900
agatgatgga tggaaatatc gatctaggat aggtatacat gttgatgcgg gttttactga 6960
tgcatataca gagatgcttt ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt 7020
cgttcattcg ttctagatcg gagtagaata ctgtttcaaa ctacctggtg tatttattaa 7080
ttttggaact gtatgtgtgt gtcatacatc ttcatagtta cgagtttaag atggatggaa 7140
atatcgatct aggataggta tacatgttga tgtgggtttt actgatgcat atacatgatg 7200
gcatatgcag catctattca tatgctctaa ccttgagtac ctatctatta taataaacaa 7260
gtatgtttta taattatttt gatcttgata tacttggatg atggcatatg cagcagctat 7320
atgtggattt ttttagccct gccttcatac gctatttatt tgcttggtac tgtttctttt 7380
gtcgatgctc accctgttgt ttggtgttac ttctgcaggg atctccgatc atgcaaaaac 7440
tcattaactc agtgcaaaac tatgcctggg gcagcaaaac ggcgttgact gaactttatg 7500
gtatggaaaa tccgtccagc cagccgatgg ccgagctgtg gatgggcgca catccgaaaa 7560
gcagttcacg agtgcagaat gccgccggag atatcgtttc actgcgtgat gtgattgaga 7620
gtgataaatc gactctgctc ggagaggccg ttgccaaacg ctttggcgaa ctgcctttcc 7680
tgttcaaagt attatgcgca gcacagccac tctccattca ggttcatcca aacaaacaca 7740
attctgaaat cggttttgcc aaagaaaatg ccgcaggtat cccgatggat gccgccgagc 7800
gtaactataa agatcctaac cacaagccgg agctggtttt tgcgctgacg cctttccttg 7860
cgatgaacgc gtttcgtgaa ttttccgaga ttgtctccct actccagccg gtcgcaggtg 7920
cacatccggc gattgctcac tttttacaac agcctgatgc cgaacgttta agcgaactgt 7980
tcgccagcct gttgaatatg cagggtgaag aaaaatcccg cgcgctggcg attttaaaat 8040
cggccctcga tagccagcag ggtgaaccgt ggcaaacgat tcgtttaatt tctgaatttt 8100
acccggaaga cagcggtctg ttctccccgc tattgctgaa tgtggtgaaa ttgaaccctg 8160
gcgaagcgat gttcctgttc gctgaaacac cgcacgctta cctgcaaggc gtggcgctgg 8220
aagtgatggc aaactccgat aacgtgctgc gtgcgggtct gacgcctaaa tacattgata 8280
ttccggaact ggttgccaat gtgaaattcg aagccaaacc ggctaaccag ttgttgaccc 8340
agccggtgaa acaaggtgca gaactggact tcccgattcc agtggatgat tttgccttct 8400
cgctgcatga ccttagtgat aaagaaacca ccattagcca gcagagtgcc gccattttgt 8460
tctgcgtcga aggcgatgca acgttgtgga aaggttctca gcagttacag cttaaaccgg 8520
gtgaatcagc gtttattgcc gccaacgaat caccggtgac tgtcaaaggc cacggccgtt 8580
tagcgcgtgt ttacaacaag ctgtaagagc ttactgaaaa aattaacatc tcttgctaag 8640
ctgggtcatg ggtcgtttaa gctgccgatg tgcctgcgtc gtctggtgcc ctctctccat 8700
atggaggttg tcaaagtatc tgctgttcgt gtcatgagtc gtgtcagtgt tggtttaata 8760
atggaccggt tgtgttgtgt gtgcgtacta cccagaacta tgacaaatca tgaataagtt 8820
tgatgtttga aattaaagcc tgtgctcatt atgttctgtc tttcagttgt ctcctaatat 8880
ttgcctccag gtactggcta tctaccgttt cttacttagg aggtgtttga atgcactaaa 8940
actaatagtt agtggctaaa attagttaaa acatccaaac accatagcta atagttgaac 9000
tattagctat ttttggaaaa ttagttaata gtgaggtagt tatttgttag ctagctaatt 9060
caactaacaa tttttagcca actaacaatt agtttcagtg cattcaaaca cccccttaat 9120
gttaacgtgg ttctatctac cgtctcctaa tatatggttg attgttcggt ttgttgctat 9180
gctattgggt tctgattgct gctagttctt gctgaatcca gaagttctcg tagtatagct 9240
cagattcata ttatttattt gagtgataag tgatccaggt tattactatg ttagctaggt 9300
tttttttaca aggataaatt atctgtgatc ataattctta tgaaagcttt atgtttcctg 9360
gaggcagtgg catgcaatgc atgacagcaa cttgatcaca ccagctgagg tagatacggt 9420
aacaaggttc ttaaatctgt tcaccaaatc attggagaac acacatacac attcttgcca 9480
gtcttggtta gagaaatttc atgacaaaat gccaaagctg tcttgactct tcacttttgg 9540
ccatgagtcg tgacttagtt tggtttaatg gaccggttct cctagcttgt tctactcaaa 9600
actgttgttg atgcgaataa gttgtgatgg ttgatctctg gattttgttt tgctctcaat 9660
agtggacgag attagatagc ctgcaggccc gggggcgcgc cctaattagc taacggccag 9720
gatcgccgcg tgagccttta gcaactagct agattaatta acgcaatctg ttattaagtt 9780
gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc 9840
ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gaattaattc 9900
tcatgtttga cagcttatca tcgactgcac ggtgcaccaa tgcttctggc gtcaggcagc 9960
catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac tgcataattc gtgtcgctca 10020
aggcgcactc ccgttctgga taatgttttt tgcgccgaca tcataacggt tctggcaaat 10080
attctgaaat gagctgttga caattaatca tccggctcgt ataatgtgtg gaattgtgag 10140
cggataacaa tttcacacag gaaacagacc atgagggaag cgttgatcgc cgaagtatcg 10200
actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc 10260
gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg 10320
ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt 10380
ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt 10440
gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga 10500
gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat 10560
ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg 10620
gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta 10680
acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg 10740
tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac 10800
tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc taggcaggct 10860
tatcttggac aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac 10920
tacgtgaaag gcgagatcac caaagtagtc ggcaaataaa gctctagtgg atctccgtac 10980
ccagggatct ggctcgcggc ggacgcacga cgccggggcg agaccatagg cgatctccta 11040
aatcaatagt agctgtaacc tcgaagcgtt tcacttgtaa caacgattga gaatttttgt 11100
cataaaattg aaatacttgg ttcgcatttt tgtcatccgc ggtcagccgc aattctgacg 11160
aactgcccat ttagctggag atgattgtac atccttcacg tgaaaatttc tcaagcgctg 11220
tgaacaaggg ttcagatttt agattgaaag gtgagccgtt gaaacacgtt cttcttgtcg 11280
atgacgacgt cgctatgcgg catcttatta ttgaatacct tacgatccac gccttcaaag 11340
tgaccgcggt agccgacagc acccagttca caagagtact ctcttccgcg acggtcgatg 11400
tcgtggttgt tgatctagat ttaggtcgtg aagatgggct cgagatcgtt cgtaatctgg 11460
cggcaaagtc tgatattcca atcataatta tcagtggcga ccgccttgag gagacggata 11520
aagttgttgc actcgagcta ggagcaagtg attttatcgc taagccgttc agtatcagag 11580
agtttctagc acgcattcgg gttgccttgc gcgtgcgccc caacgttgtc cgctccaaag 11640
accgacggtc tttttgtttt actgactgga cacttaatct caggcaacgt cgcttgatgt 11700
ccgaagctgg cggtgaggtg aaacttacgg caggtgagtt caatcttctc ctcgcgtttt 11760
tagagaaacc ccgcgacgtt ctatcgcgcg agcaacttct cattgccagt cgagtacgcg 11820
acgaggaggt ttatgacagg agtatagatg ttctcatttt gaggctgcgc cgcaaacttg 11880
aggcagatcc gtcaagccct caactgataa aaacagcaag aggtgccggt tatttctttg 11940
acgcggacgt gcaggtttcg cacgggggga cgatggcagc ctgagccaat tcccagatcc 12000
ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct 12060
gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga 12120
ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc 12180
ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca 12240
accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat 12300
ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta 12360
cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg 12420
ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg 12480
ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa 12540
gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg 12600
gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt 12660
gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg 12720
gcggccggag tacatcgaga tcgagctggc tgattggatg taccgcgaga tcacagaagg 12780
caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg 12840
ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt 12900
caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt 12960
gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca 13020
ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg 13080
ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa 13140
aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg 13200
gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac 13260
tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac 13320
tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca 13380
aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat 13440
cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg 13500
acaagccgcg ccgtcgccac tcgaccgccg gcgctgaggt ctgcctcgtg aagaaggtgt 13560
tgctgactca taccaggcct gaatcgcccc atcatccagc cagaaagtga gggagccacg 13620
gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt gctttgccac 13680
ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag caaaagttcg 13740
atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac 13800
caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc 13860
atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac 13920
tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt 13980
ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa 14040
tcaccatgag tgacgactga atccggtgag aatggcaaaa gctctgcatt aatgaatcgg 14100
ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 14160
ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 14220
acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 14280
aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 14340
tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 14400
aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 14460
gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 14520
acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 14580
accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 14640
ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 14700
gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 14760
aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 14820
ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 14880
gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 14940
cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 15000
cttcacctag atccttttga tccggaatta 15030
<210> 51
<211> 15033
<212> DNA
<213> 人工序列
<220>
<223> 载体24376
<400> 51
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta 1260
gatcggcgtt ccggtccata gttagggccc ggtagttcta cttctgttca tgtttgtgtt 1320
agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg 1380
tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct 1440
ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt 1500
ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt 1560
catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg 1620
gagtagaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt 1680
gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag 1740
gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg 1800
ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact 1860
gtttcaaact acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt 1920
catagttacg agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg 1980
tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc 2040
ttgagtacct atctattata ataaacaagt atgttttata attattttga tcttgatata 2100
cttggatgat ggcatatcca gcagctatat gtggattttt ttagccctgc cttcatacgc 2160
tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt 2220
ctgcaggatc ctctaagtag cagccatgga caacaacccc aacatcaacg agtgcatccc 2280
ctacaactgc ctgagcaacc ccgaggtgga ggtgctgggc ggcgagcgca tcgagaccgg 2340
ctacaccccc atcgacatca gcctgagcct gacccagttc ctgctgagcg agttcgtgcc 2400
cggcgccggc ttcgtgctgg gcctggtgga catcatctgg ggcatcttcg gccccagcca 2460
gtgggacgcc ttcctggtgc agatcgagca gttgataaac caacgcatag aggaattcgc 2520
ccgcaaccag gccatcagcc gcctggaggg cctgagcaac ctgtaccaaa tctacgccga 2580
gagcttccgc gagtgggagg ccgaccccac caaccccgcc ctgcgcgagg agatgcgcat 2640
ccagttcaac gacatgaaca gcgccctgac caccgccatc cccctgttcg ccgtgcagaa 2700
ctaccaggtg cccctgctga gcgtgtacgt gcaggccgcc aacctgcacc tgagcgtgct 2760
gcgcgacgtc agcgtgttcg gccagcgctg gggcttcgac gccgccacca tcaacagccg 2820
ctacaacgac ctgacccgcc tgatcggcaa ctacaccgac cacgccgtgc gctggtacaa 2880
caccggcctg gagcgcgtgt gggggcccga cagccgcgac tggatcaggt acaaccagtt 2940
ccgccgcgag ctgaccctga ccgtgctgga catcgtgagc ctgttcccca actacgacag 3000
ccgcacctac cccatccgca ccgtgagcca gctgacccgc gagatttaca ccaaccccgt 3060
gctggagaac ttcgacggca gcttccgcgg cagcgcccag ggcatcgagg gcagcatccg 3120
cagcccccac ctgatggaca tcctgaacag catcaccatc tacaccgacg cccaccgcgg 3180
cgagtactac tggagcggcc accagatcat ggccagcccc gtcggcttca gcggccccga 3240
gttcaccttc cccctgtacg gcacgatggg caacgctgca cctcagcagc gcatcgtggc 3300
acagctgggc cagggagtgt accgcaccct gagcagcacc ctgtaccgtc gacctttcaa 3360
catcggcatc aacaaccagc agctgagcgt gctggacggc accgagttcg cctacggcac 3420
cagcagcaac ctgcccagcg ccgtgtaccg caagagcggc accgtggaca gcctggacga 3480
gatcccccct cagaacaaca acgtgccacc tcgacagggc ttcagccacc gtctgagcca 3540
cgtgagcatg ttccgcagtg gcttcagcaa cagcagcgtg agcatcatcc gtgcacctat 3600
gttcagctgg attcaccgca gtgccgagtt caacaacatc atccccagca gccagatcac 3660
ccagatcccc ctgaccaaga gcaccaacct gggcagcggc accagcgtgg tgaagggccc 3720
cggcttcacc ggcggcgaca tcctgcgccg caccagcccc ggccagatca gcaccctgcg 3780
cgtgaacatc accgcccccc tgagccagcg ctaccgcgtc cgcatccgct acgccagcac 3840
caccaacctg cagttccaca ccagcatcga cggccgcccc atcaaccagg gcaacttcag 3900
cgccaccatg agcagcggca gcaacctgca gagcggcagc ttccgcaccg tgggcttcac 3960
cacccccttc aacttcagca acggcagcag cgtgttcacc ctgagcgccc acgtgttcaa 4020
cagcggcaac gaggtgtaca tcgaccgcat cgagttcgtg cccgccgagg tgaccttcga 4080
ggccgagtac gacctggaga gggctcagaa ggccgtgaac gagctgttca ccagcagcaa 4140
ccagatcggc ctgaagaccg acgtgaccga ctaccacatc gaccaggtga gcaacctggt 4200
ggagtgctta agcgacgagt tctgcctgga cgagaagaag gagctgagcg agaaggtgaa 4260
gcacgccaag cgcctgagcg acgagcgcaa cctgctgcag gaccccaact tccgcggcat 4320
caaccgccag ctggaccgcg gctggcgagg cagcaccgat atcaccatcc agggcggcga 4380
cgacgtgttc aaggagaact acgtgaccct gctgggcacc ttcgacgagt gctaccccac 4440
ctacctgtac cagaagatcg acgagagcaa gctgaaggcc tacacccgct accagctgcg 4500
cggctacatc gaggacagcc aggacctgga aatctacctg atccgctaca acgcgaagca 4560
cgagaccgtg aacgtgcccg gcaccggcag cctgtggccc ctgagcgccc ccagccccat 4620
cggcaagtgc actagcgacg accccaaagg acaggtttcc cacagcttta ttgaacaatt 4680
gtttgcatct agtcaccaca gccaccactt cagcctggac atcgacgtgg gctgcaccga 4740
cctgaacgag gacctgggcg tgtgggtgat cttcaagatc aagacccagg acggccacgc 4800
ccgcctgggc aatctagagt tcctggagga gaagcccctg gtgggcgagg ccctggcccg 4860
cgtgaagcgt gctgagaaga agtggcgcga caagcgcgag aagctggagt gggagaccaa 4920
catcgtgtac aaggaggcca aggagagcgt ggacgccctg ttcgtgaaca gccagtacga 4980
ccgcctgcag gccgacacca acatcgccat gatccacgcc gccgacaagc gcgtgcacag 5040
cattcgcgag gcctacctgc ccgagctgag cgtgatcccc ggtgtgaacg ccgccatctt 5100
cgaggaactc gagggccgca tctaggagct ctatgatcgt tcaaacattt ggcaataaag 5160
tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa 5220
ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt 5280
tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc 5340
aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcaat agactacgga 5400
ccgcgatcgc ttaattaagc ttgcatgcct gcagtgcagc gtgacccggt cgtgcccctc 5460
tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt 5520
cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg 5580
aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac 5640
agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc 5700
tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc 5760
atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt 5820
ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt 5880
ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt 5940
aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag 6000
ataatgccag cctgttaaac gccgccgacg agtctaacgg acaccaacca gcgaaccagc 6060
agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc 6120
cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg 6180
tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacc 6240
ggcagctacg ggggattcct ttcccaccgc tccttcgctt tcccttcctc gcccgccgta 6300
ataaatagac accccctcca caccctcttt ccccaacctc gtgttgttcg gagcgcacac 6360
acacacaacc agatctcccc caaatccacc cgtcggcacc tccgcttcaa ggtacgccgc 6420
tcgtcctccc cccccccccc tctctacctt ctctagatcg gcgttccggt ccatagttag 6480
ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt 6540
gctgttagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga ttgctaactt 6600
gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag acgggatcga 6660
tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc tttatttcaa 6720
tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt cttggttgtg 6780
atgatgtggt ctggttgggc ggtcgttcta gatcggagta gaattctgtt tcaaactacc 6840
tggtggattt attaattttg gatctgtatg tgtgtgccat acatattcat agttacgaat 6900
tgaagatgat ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac 6960
tgatgcatat acagagatgc tttttgttcg cttggttgtg atgatgtggt gtggttgggc 7020
ggtcgttcat tcgttctaga tcggagtaga atactgtttc aaactacctg gtgtatttat 7080
taattttgga actgtatgtg tgtgtcatac atcttcatag ttacgagttt aagatggatg 7140
gaaatatcga tctaggatag gtatacatgt tgatgtgggt tttactgatg catatacatg 7200
atggcatatg cagcatctat tcatatgctc taaccttgag tacctatcta ttataataaa 7260
caagtatgtt ttataattat tttgatcttg atatacttgg atgatggcat atgcagcagc 7320
tatatgtgga tttttttagc cctgccttca tacgctattt atttgcttgg tactgtttct 7380
tttgtcgatg ctcaccctgt tgtttggtgt tacttctgca gggatctccg atcatgcaaa 7440
aactcattaa ctcagtgcaa aactatgcct ggggcagcaa aacggcgttg actgaacttt 7500
atggtatgga aaatccgtcc agccagccga tggccgagct gtggatgggc gcacatccga 7560
aaagcagttc acgagtgcag aatgccgccg gagatatcgt ttcactgcgt gatgtgattg 7620
agagtgataa atcgactctg ctcggagagg ccgttgccaa acgctttggc gaactgcctt 7680
tcctgttcaa agtattatgc gcagcacagc cactctccat tcaggttcat ccaaacaaac 7740
acaattctga aatcggtttt gccaaagaaa atgccgcagg tatcccgatg gatgccgccg 7800
agcgtaacta taaagatcct aaccacaagc cggagctggt ttttgcgctg acgcctttcc 7860
ttgcgatgaa cgcgtttcgt gaattttccg agattgtctc cctactccag ccggtcgcag 7920
gtgcacatcc ggcgattgct cactttttac aacagcctga tgccgaacgt ttaagcgaac 7980
tgttcgccag cctgttgaat atgcagggtg aagaaaaatc ccgcgcgctg gcgattttaa 8040
aatcggccct cgatagccag cagggtgaac cgtggcaaac gattcgttta atttctgaat 8100
tttacccgga agacagcggt ctgttctccc cgctattgct gaatgtggtg aaattgaacc 8160
ctggcgaagc gatgttcctg ttcgctgaaa caccgcacgc ttacctgcaa ggcgtggcgc 8220
tggaagtgat ggcaaactcc gataacgtgc tgcgtgcggg tctgacgcct aaatacattg 8280
atattccgga actggttgcc aatgtgaaat tcgaagccaa accggctaac cagttgttga 8340
cccagccggt gaaacaaggt gcagaactgg acttcccgat tccagtggat gattttgcct 8400
tctcgctgca tgaccttagt gataaagaaa ccaccattag ccagcagagt gccgccattt 8460
tgttctgcgt cgaaggcgat gcaacgttgt ggaaaggttc tcagcagtta cagcttaaac 8520
cgggtgaatc agcgtttatt gccgccaacg aatcaccggt gactgtcaaa ggccacggcc 8580
gtttagcgcg tgtttacaac aagctgtaag agcttactga aaaaattaac atctcttgct 8640
aagctgggtc atgggtcgtt taagctgccg atgtgcctgc gtcgtctggt gccctctctc 8700
catatggagg ttgtcaaagt atctgctgtt cgtgtcatga gtcgtgtcag tgttggttta 8760
ataatggacc ggttgtgttg tgtgtgcgta ctacccagaa ctatgacaaa tcatgaataa 8820
gtttgatgtt tgaaattaaa gcctgtgctc attatgttct gtctttcagt tgtctcctaa 8880
tatttgcctc caggtactgg ctatctaccg tttcttactt aggaggtgtt tgaatgcact 8940
aaaactaata gttagtggct aaaattagtt aaaacatcca aacaccatag ctaatagttg 9000
aactattagc tatttttgga aaattagtta atagtgaggt agttatttgt tagctagcta 9060
attcaactaa caatttttag ccaactaaca attagtttca gtgcattcaa acaccccctt 9120
aatgttaacg tggttctatc taccgtctcc taatatatgg ttgattgttc ggtttgttgc 9180
tatgctattg ggttctgatt gctgctagtt cttgctgaat ccagaagttc tcgtagtata 9240
gctcagattc atattattta tttgagtgat aagtgatcca ggttattact atgttagcta 9300
ggtttttttt acaaggataa attatctgtg atcataattc ttatgaaagc tttatgtttc 9360
ctggaggcag tggcatgcaa tgcatgacag caacttgatc acaccagctg aggtagatac 9420
ggtaacaagg ttcttaaatc tgttcaccaa atcattggag aacacacata cacattcttg 9480
ccagtcttgg ttagagaaat ttcatgacaa aatgccaaag ctgtcttgac tcttcacttt 9540
tggccatgag tcgtgactta gtttggttta atggaccggt tctcctagct tgttctactc 9600
aaaactgttg ttgatgcgaa taagttgtga tggttgatct ctggattttg ttttgctctc 9660
aatagtggac gagattagat agcctgcagg cccgggggcg cgccctaatt agctaacggc 9720
caggatcgcc gcgtgagcct ttagcaacta gctagattaa ttaacgcaat ctgttattaa 9780
gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc 9840
tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca tcagaattaa 9900
ttctcatgtt tgacagctta tcatcgactg cacggtgcac caatgcttct ggcgtcaggc 9960
agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc 10020
tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca 10080
aatattctga aatgagctgt tgacaattaa tcatccggct cgtataatgt gtggaattgt 10140
gagcggataa caatttcaca caggaaacag accatgaggg aagcgttgat cgccgaagta 10200
tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg 10260
gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat 10320
ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac 10380
cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc 10440
attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt 10500
ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt 10560
gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg 10620
gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc 10680
ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg 10740
ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc 10800
gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctaggcag 10860
gcttatcttg gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt 10920
cactacgtga aaggcgagat caccaaagta gtcggcaaat aaagctctag tggatctccg 10980
tacccaggga tctggctcgc ggcggacgca cgacgccggg gcgagaccat aggcgatctc 11040
ctaaatcaat agtagctgta acctcgaagc gtttcacttg taacaacgat tgagaatttt 11100
tgtcataaaa ttgaaatact tggttcgcat ttttgtcatc cgcggtcagc cgcaattctg 11160
acgaactgcc catttagctg gagatgattg tacatccttc acgtgaaaat ttctcaagcg 11220
ctgtgaacaa gggttcagat tttagattga aaggtgagcc gttgaaacac gttcttcttg 11280
tcgatgacga cgtcgctatg cggcatctta ttattgaata ccttacgatc cacgccttca 11340
aagtgaccgc ggtagccgac agcacccagt tcacaagagt actctcttcc gcgacggtcg 11400
atgtcgtggt tgttgatcta gatttaggtc gtgaagatgg gctcgagatc gttcgtaatc 11460
tggcggcaaa gtctgatatt ccaatcataa ttatcagtgg cgaccgcctt gaggagacgg 11520
ataaagttgt tgcactcgag ctaggagcaa gtgattttat cgctaagccg ttcagtatca 11580
gagagtttct agcacgcatt cgggttgcct tgcgcgtgcg ccccaacgtt gtccgctcca 11640
aagaccgacg gtctttttgt tttactgact ggacacttaa tctcaggcaa cgtcgcttga 11700
tgtccgaagc tggcggtgag gtgaaactta cggcaggtga gttcaatctt ctcctcgcgt 11760
ttttagagaa accccgcgac gttctatcgc gcgagcaact tctcattgcc agtcgagtac 11820
gcgacgagga ggtttatgac aggagtatag atgttctcat tttgaggctg cgccgcaaac 11880
ttgaggcaga tccgtcaagc cctcaactga taaaaacagc aagaggtgcc ggttatttct 11940
ttgacgcgga cgtgcaggtt tcgcacgggg ggacgatggc agcctgagcc aattcccaga 12000
tccccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc 12060
gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat 12120
cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga 12180
atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga 12240
gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat 12300
catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg 12360
ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt 12420
gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata 12480
ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact 12540
caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat 12600
tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct 12660
ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac 12720
cgggcggccg gagtacatcg agatcgagct ggctgattgg atgtaccgcg agatcacaga 12780
aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat 12840
cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt 12900
gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac 12960
cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg 13020
gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc 13080
cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg 13140
aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa 13200
ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt 13260
gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa 13320
aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct 13380
gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc 13440
tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg 13500
cggacaagcc gcgccgtcgc cactcgaccg ccggcgctga ggtctgcctc gtgaagaagg 13560
tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag tgagggagcc 13620
acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact tttgctttgc 13680
cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact cagcaaaagt 13740
tcgatttatt caacaaagcc gccgtcccgt caagtcagcg taatgctctg ccagtgttac 13800
aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta 13860
ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa 13920
aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact 13980
cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag 14040
aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagctctgc attaatgaat 14100
cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 14160
tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 14220
aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 14280
gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 14340
ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 14400
ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 14460
gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 14520
ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 14580
cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 14640
cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 14700
gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 14760
aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 14820
tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 14880
gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 14940
tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 15000
gatcttcacc tagatccttt tgatccggaa tta 15033
<210> 52
<211> 15030
<212> DNA
<213> 人工序列
<220>
<223> 载体24377
<400> 52
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta 1260
gatcggcgtt ccggtccata gttagggccc ggtagttcta cttctgttca tgtttgtgtt 1320
agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg 1380
tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct 1440
ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt 1500
ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt 1560
catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg 1620
gagtagaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt 1680
gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag 1740
gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg 1800
ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact 1860
gtttcaaact acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt 1920
catagttacg agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg 1980
tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc 2040
ttgagtacct atctattata ataaacaagt atgttttata attattttga tcttgatata 2100
cttggatgat ggcatatcca gcagctatat gtggattttt ttagccctgc cttcatacgc 2160
tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt 2220
ctgcaggatc ctctaagtag cagccatgga caacaacccc aacatcaacg agtgcatccc 2280
ctacaactgc ctgagcaacc ccgaggtgga ggtgctgggc ggcgagcgca tcgagaccgg 2340
ctacaccccc atcgacatca gcctgagcct gacccagttc ctgctgagcg agttcgtgcc 2400
cggcgccggc ttcgtgctgg gcctggtgga catcatctgg ggcatcttcg gccccagcca 2460
gtgggacgcc ttcctggtgc agatcgagca gttgataaac caacgcatag aggaattcgc 2520
ccgcaaccag gccatcagcc gcctggaggg cctgagcaac ctgtaccaaa tctacgccga 2580
gagcttccgc gagtgggagg ccgaccccac caaccccgcc ctgcgcgagg agatgcgcat 2640
ccagttcaac gacatgaaca gcgccctgac caccgccatc cccctgttcg ccgtgcagaa 2700
ctaccaggtg cccctgctga gcgtgtacgt gcaggccgcc aacctgcacc tgagcgtgct 2760
gcgcgacgtc agcgtgttcg gccagcgctg gggcttcgac gccgccacca tcaacagccg 2820
ctacaacgac ctgacccgcc tgatcggcaa ctacaccgac cacgccgtgc gctggtacaa 2880
caccggcctg gagcgcgtgt gggggcccga cagccgcgac tggatcaggt acaaccagtt 2940
ccgccgcgag ctgaccctga ccgtgctgga catcgtgagc ctgttcccca actacgacag 3000
ccgcacctac cccatccgca ccgtgagcca gctgacccgc gagatttaca ccaaccccgt 3060
gctggagaac ttcgacggca gcttccgcgg cagcgcccag ggcatcgagg gcagcatccg 3120
cagcccccac ctgatggaca tcctgaacag catcaccatc tacaccgacg cccaccgcgg 3180
cgagtactac tggagcggcc accagatcat ggccagcccc gtcggcttca gcggccccga 3240
gttcaccttc cccctgtacg gcacgatggg caacgctgca cctcagcagc gcatcgtggc 3300
acagctgggc cagggagtgt accgcaccct gagcagcacc ctgtaccgtc gacctttcaa 3360
catcggcatc aacaaccagc agctgagcgt gctggacggc accgagttcg cctacggcac 3420
cagcagcaac ctgcccagcg ccgtgtaccg caagagcggc accgtggaca gcctggacga 3480
gatcccccct cagaacaaca acgtgccacc tcgacagggc ttcagccacc gtctgagcca 3540
cgtgagcatg ttccgcagtg gcttcagcaa cagcagcgtg agcatcatcc gtgcacctat 3600
gttcagctgg attcaccgca gtgccgagtt caacaacatc atccccagca gccagatcac 3660
ccagatcccc ctgaccaaga gcaccaacct gggcagcggc accagcgtgg tgaagggccc 3720
cggcttcacc ggcggcgaca tcctgcgccg caccagcccc ggccagatca gcaccctgcg 3780
cgtgaacatc accgcccccc tgagccagcg ctaccgcgtc cgcatccgct acgccagcac 3840
caccaacctg cagttccaca ccagcatcga cggccgcccc atcaaccagg gcaacttcag 3900
cgccaccatg agcagcggca gcaacctgca gagcggcagc ttccgcaccg tgggcttcac 3960
cacccccttc aacttcagca acggcagcag cgtgttcacc ctgagcgccc acgtgttcaa 4020
cagcggcaac gaggtgtaca tcgaccgcat cgagttcgtg cccgccgagg tgaccttcga 4080
ggccgagtac gacctggaga gggctcagaa ggccgtgaac gagctgttca ccagcagcaa 4140
ccagatcggc ctgaagaccg acgtgaccga ctaccacatc gaccaggtga gcaacctggt 4200
ggagtgctta agcgacgagt tctgcctgga cgagaagaag gagctgagcg agaaggtgaa 4260
gcacgccaag cgcctgagcg acgagcgcaa cctgctgcag gaccccaact tccgcggcat 4320
caaccgccag ctggaccgcg gctggcgagg cagcaccgat atcaccatcc agggcggcga 4380
cgacgtgttc aaggagaact acgtgaccct gctgggcacc ttcgacgagt gctaccccac 4440
ctacctgtac cagaagatcg acgagagcaa gctgaaggcc tacacccgct accagctgcg 4500
cggctacatc gaggacagcc aggacctgga aatctacctg atccgctaca acgcgaagca 4560
cgagaccgtg aacgtgcccg gcaccggcag cctgtggccc ctgagcgccc ccagccccat 4620
cggcaagtgc actagtcctt tctctctctt tctttctctc tgctttattg aacttctcaa 4680
agttactagt caccacagcc accacttcag cctggacatc gacgtgggct gcaccgacct 4740
gaacgaggac ctgggcgtgt gggtgatctt caagatcaag acccaggacg gccacgcccg 4800
cctgggcaat ctagagttcc tggaggagaa gcccctggtg ggcgaggccc tggcccgcgt 4860
gaagcgtgct gagaagaagt ggcgcgacaa gcgcgagaag ctggagtggg agaccaacat 4920
cgtgtacaag gaggccaagg agagcgtgga cgccctgttc gtgaacagcc agtacgaccg 4980
cctgcaggcc gacaccaaca tcgccatgat ccacgccgcc gacaagcgcg tgcacagcat 5040
tcgcgaggcc tacctgcccg agctgagcgt gatccccggt gtgaacgccg ccatcttcga 5100
ggaactcgag ggccgcatct aggagctcta tgatcgttca aacatttggc aataaagttt 5160
cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta 5220
cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat 5280
gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa 5340
ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcaataga ctacggaccg 5400
cgatcgctta attaagcttg catgcctgca gtgcagcgtg acccggtcgt gcccctctct 5460
agagataatg agcattgcat gtctaagtta taaaaaatta ccacatattt tttttgtcac 5520
acttgtttga agtgcagttt atctatcttt atacatatat ttaaacttta ctctacgaat 5580
aatataatct atagtactac aataatatca gtgttttaga gaatcatata aatgaacagt 5640
tagacatggt ctaaaggaca attgagtatt ttgacaacag gactctacag ttttatcttt 5700
ttagtgtgca tgtgttctcc tttttttttg caaatagctt cacctatata atacttcatc 5760
cattttatta gtacatccat ttagggttta gggttaatgg tttttataga ctaatttttt 5820
tagtacatct attttattct attttagcct ctaaattaag aaaactaaaa ctctatttta 5880
gtttttttat ttaataattt agatataaaa tagaataaaa taaagtgact aaaaattaaa 5940
caaataccct ttaagaaatt aaaaaaacta aggaaacatt tttcttgttt cgagtagata 6000
atgccagcct gttaaacgcc gccgacgagt ctaacggaca ccaaccagcg aaccagcagc 6060
gtcgcgtcgg gccaagcgaa gcagacggca cggcatctct gtcgctgcct ctggacccct 6120
ctcgagagtt ccgctccacc gttggacttg ctccgctgtc ggcatccaga aattgcgtgg 6180
cggagcggca gacgtgagcc ggcacggcag gcggcctcct cctcctctca cggcaccggc 6240
agctacgggg gattcctttc ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata 6300
aatagacacc ccctccacac cctctttccc caacctcgtg ttgttcggag cgcacacaca 6360
cacaaccaga tctcccccaa atccacccgt cggcacctcc gcttcaaggt acgccgctcg 6420
tcctcccccc ccccccctct ctaccttctc tagatcggcg ttccggtcca tagttagggc 6480
ccggtagttc tacttctgtt catgtttgtg ttagatccgt gtttgtgtta gatccgtgct 6540
gttagcgttc gtacacggat gcgacctgta cgtcagacac gttctgattg ctaacttgcc 6600
agtgtttctc tttggggaat cctgggatgg ctctagccgt tccgcagacg ggatcgattt 6660
catgattttt tttgtttcgt tgcatagggt ttggtttgcc cttttccttt atttcaatat 6720
atgccgtgca cttgtttgtc gggtcatctt ttcatgcttt tttttgtctt ggttgtgatg 6780
atgtggtctg gttgggcggt cgttctagat cggagtagaa ttctgtttca aactacctgg 6840
tggatttatt aattttggat ctgtatgtgt gtgccataca tattcatagt tacgaattga 6900
agatgatgga tggaaatatc gatctaggat aggtatacat gttgatgcgg gttttactga 6960
tgcatataca gagatgcttt ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt 7020
cgttcattcg ttctagatcg gagtagaata ctgtttcaaa ctacctggtg tatttattaa 7080
ttttggaact gtatgtgtgt gtcatacatc ttcatagtta cgagtttaag atggatggaa 7140
atatcgatct aggataggta tacatgttga tgtgggtttt actgatgcat atacatgatg 7200
gcatatgcag catctattca tatgctctaa ccttgagtac ctatctatta taataaacaa 7260
gtatgtttta taattatttt gatcttgata tacttggatg atggcatatg cagcagctat 7320
atgtggattt ttttagccct gccttcatac gctatttatt tgcttggtac tgtttctttt 7380
gtcgatgctc accctgttgt ttggtgttac ttctgcaggg atctccgatc atgcaaaaac 7440
tcattaactc agtgcaaaac tatgcctggg gcagcaaaac ggcgttgact gaactttatg 7500
gtatggaaaa tccgtccagc cagccgatgg ccgagctgtg gatgggcgca catccgaaaa 7560
gcagttcacg agtgcagaat gccgccggag atatcgtttc actgcgtgat gtgattgaga 7620
gtgataaatc gactctgctc ggagaggccg ttgccaaacg ctttggcgaa ctgcctttcc 7680
tgttcaaagt attatgcgca gcacagccac tctccattca ggttcatcca aacaaacaca 7740
attctgaaat cggttttgcc aaagaaaatg ccgcaggtat cccgatggat gccgccgagc 7800
gtaactataa agatcctaac cacaagccgg agctggtttt tgcgctgacg cctttccttg 7860
cgatgaacgc gtttcgtgaa ttttccgaga ttgtctccct actccagccg gtcgcaggtg 7920
cacatccggc gattgctcac tttttacaac agcctgatgc cgaacgttta agcgaactgt 7980
tcgccagcct gttgaatatg cagggtgaag aaaaatcccg cgcgctggcg attttaaaat 8040
cggccctcga tagccagcag ggtgaaccgt ggcaaacgat tcgtttaatt tctgaatttt 8100
acccggaaga cagcggtctg ttctccccgc tattgctgaa tgtggtgaaa ttgaaccctg 8160
gcgaagcgat gttcctgttc gctgaaacac cgcacgctta cctgcaaggc gtggcgctgg 8220
aagtgatggc aaactccgat aacgtgctgc gtgcgggtct gacgcctaaa tacattgata 8280
ttccggaact ggttgccaat gtgaaattcg aagccaaacc ggctaaccag ttgttgaccc 8340
agccggtgaa acaaggtgca gaactggact tcccgattcc agtggatgat tttgccttct 8400
cgctgcatga ccttagtgat aaagaaacca ccattagcca gcagagtgcc gccattttgt 8460
tctgcgtcga aggcgatgca acgttgtgga aaggttctca gcagttacag cttaaaccgg 8520
gtgaatcagc gtttattgcc gccaacgaat caccggtgac tgtcaaaggc cacggccgtt 8580
tagcgcgtgt ttacaacaag ctgtaagagc ttactgaaaa aattaacatc tcttgctaag 8640
ctgggtcatg ggtcgtttaa gctgccgatg tgcctgcgtc gtctggtgcc ctctctccat 8700
atggaggttg tcaaagtatc tgctgttcgt gtcatgagtc gtgtcagtgt tggtttaata 8760
atggaccggt tgtgttgtgt gtgcgtacta cccagaacta tgacaaatca tgaataagtt 8820
tgatgtttga aattaaagcc tgtgctcatt atgttctgtc tttcagttgt ctcctaatat 8880
ttgcctccag gtactggcta tctaccgttt cttacttagg aggtgtttga atgcactaaa 8940
actaatagtt agtggctaaa attagttaaa acatccaaac accatagcta atagttgaac 9000
tattagctat ttttggaaaa ttagttaata gtgaggtagt tatttgttag ctagctaatt 9060
caactaacaa tttttagcca actaacaatt agtttcagtg cattcaaaca cccccttaat 9120
gttaacgtgg ttctatctac cgtctcctaa tatatggttg attgttcggt ttgttgctat 9180
gctattgggt tctgattgct gctagttctt gctgaatcca gaagttctcg tagtatagct 9240
cagattcata ttatttattt gagtgataag tgatccaggt tattactatg ttagctaggt 9300
tttttttaca aggataaatt atctgtgatc ataattctta tgaaagcttt atgtttcctg 9360
gaggcagtgg catgcaatgc atgacagcaa cttgatcaca ccagctgagg tagatacggt 9420
aacaaggttc ttaaatctgt tcaccaaatc attggagaac acacatacac attcttgcca 9480
gtcttggtta gagaaatttc atgacaaaat gccaaagctg tcttgactct tcacttttgg 9540
ccatgagtcg tgacttagtt tggtttaatg gaccggttct cctagcttgt tctactcaaa 9600
actgttgttg atgcgaataa gttgtgatgg ttgatctctg gattttgttt tgctctcaat 9660
agtggacgag attagatagc ctgcaggccc gggggcgcgc cctaattagc taacggccag 9720
gatcgccgcg tgagccttta gcaactagct agattaatta acgcaatctg ttattaagtt 9780
gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc 9840
ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gaattaattc 9900
tcatgtttga cagcttatca tcgactgcac ggtgcaccaa tgcttctggc gtcaggcagc 9960
catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac tgcataattc gtgtcgctca 10020
aggcgcactc ccgttctgga taatgttttt tgcgccgaca tcataacggt tctggcaaat 10080
attctgaaat gagctgttga caattaatca tccggctcgt ataatgtgtg gaattgtgag 10140
cggataacaa tttcacacag gaaacagacc atgagggaag cgttgatcgc cgaagtatcg 10200
actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc 10260
gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg 10320
ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt 10380
ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt 10440
gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga 10500
gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat 10560
ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg 10620
gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta 10680
acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg 10740
tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac 10800
tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc taggcaggct 10860
tatcttggac aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac 10920
tacgtgaaag gcgagatcac caaagtagtc ggcaaataaa gctctagtgg atctccgtac 10980
ccagggatct ggctcgcggc ggacgcacga cgccggggcg agaccatagg cgatctccta 11040
aatcaatagt agctgtaacc tcgaagcgtt tcacttgtaa caacgattga gaatttttgt 11100
cataaaattg aaatacttgg ttcgcatttt tgtcatccgc ggtcagccgc aattctgacg 11160
aactgcccat ttagctggag atgattgtac atccttcacg tgaaaatttc tcaagcgctg 11220
tgaacaaggg ttcagatttt agattgaaag gtgagccgtt gaaacacgtt cttcttgtcg 11280
atgacgacgt cgctatgcgg catcttatta ttgaatacct tacgatccac gccttcaaag 11340
tgaccgcggt agccgacagc acccagttca caagagtact ctcttccgcg acggtcgatg 11400
tcgtggttgt tgatctagat ttaggtcgtg aagatgggct cgagatcgtt cgtaatctgg 11460
cggcaaagtc tgatattcca atcataatta tcagtggcga ccgccttgag gagacggata 11520
aagttgttgc actcgagcta ggagcaagtg attttatcgc taagccgttc agtatcagag 11580
agtttctagc acgcattcgg gttgccttgc gcgtgcgccc caacgttgtc cgctccaaag 11640
accgacggtc tttttgtttt actgactgga cacttaatct caggcaacgt cgcttgatgt 11700
ccgaagctgg cggtgaggtg aaacttacgg caggtgagtt caatcttctc ctcgcgtttt 11760
tagagaaacc ccgcgacgtt ctatcgcgcg agcaacttct cattgccagt cgagtacgcg 11820
acgaggaggt ttatgacagg agtatagatg ttctcatttt gaggctgcgc cgcaaacttg 11880
aggcagatcc gtcaagccct caactgataa aaacagcaag aggtgccggt tatttctttg 11940
acgcggacgt gcaggtttcg cacgggggga cgatggcagc ctgagccaat tcccagatcc 12000
ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct 12060
gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga 12120
ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc 12180
ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca 12240
accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat 12300
ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta 12360
cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg 12420
ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg 12480
ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa 12540
gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg 12600
gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt 12660
gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg 12720
gcggccggag tacatcgaga tcgagctggc tgattggatg taccgcgaga tcacagaagg 12780
caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg 12840
ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt 12900
caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt 12960
gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca 13020
ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg 13080
ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa 13140
aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg 13200
gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac 13260
tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac 13320
tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca 13380
aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat 13440
cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg 13500
acaagccgcg ccgtcgccac tcgaccgccg gcgctgaggt ctgcctcgtg aagaaggtgt 13560
tgctgactca taccaggcct gaatcgcccc atcatccagc cagaaagtga gggagccacg 13620
gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt gctttgccac 13680
ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag caaaagttcg 13740
atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac 13800
caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc 13860
atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac 13920
tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt 13980
ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa 14040
tcaccatgag tgacgactga atccggtgag aatggcaaaa gctctgcatt aatgaatcgg 14100
ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 14160
ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 14220
acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 14280
aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 14340
tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 14400
aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 14460
gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 14520
acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 14580
accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 14640
ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 14700
gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 14760
aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 14820
ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 14880
gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 14940
cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 15000
cttcacctag atccttttga tccggaatta 15030
<210> 53
<211> 15033
<212> DNA
<213> 人工序列
<220>
<223> 载体24379
<400> 53
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctagcatc cttttcattg ataagaagag caccgttcac tccaactgca 1260
aacttgtggc taggtacgcc gctcgtcctc cccccccccc cctctctacc ttctctagat 1320
cggcgttccg gtccatagtt agggcccggt agttctactt ctgttcatgt ttgtgttaga 1380
tccgtgtttg tgttagatcc gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca 1440
gacacgttct gattgctaac ttgccagtgt ttctctttgg ggaatcctgg gatggctcta 1500
gccgttccgc agacgggatc gatttcatga ttttttttgt ttcgttgcat agggtttggt 1560
ttgccctttt cctttatttc aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat 1620
gctttttttt gtcttggttg tgatgatgtg gtctggttgg gcggtcgttc tagatcggag 1680
tagaattctg tttcaaacta cctggtggat ttattaattt tggatctgta tgtgtgtgcc 1740
atacatattc atagttacga attgaagatg atggatggaa atatcgatct aggataggta 1800
tacatgttga tgcgggtttt actgatgcat atacagagat gctttttgtt cgcttggttg 1860
tgatgatgtg gtgtggttgg gcggtcgttc attcgttcta gatcggagta gaatactgtt 1920
tcaaactacc tggtgtattt attaattttg gaactgtatg tgtgtgtcat acatcttcat 1980
agttacgagt ttaagatgga tggaaatatc gatctaggat aggtatacat gttgatgtgg 2040
gttttactga tgcatataca tgatggcata tgcagcatct attcatatgc tctaaccttg 2100
agtacctatc tattataata aacaagtatg ttttataatt attttgatct tgatatactt 2160
ggatgatggc atatccagca gctatatgtg gattttttta gccctgcctt catacgctat 2220
ttatttgctt ggtactgttt cttttgtcga tgctcaccct gttgtttggt gttacttctg 2280
caggatcctc taagtagcag ccatggacaa caaccccaac atcaacgagt gcatccccta 2340
caactgcctg agcaaccccg aggtggaggt gctgggcggc gagcgcatcg agaccggcta 2400
cacccccatc gacatcagcc tgagcctgac ccagttcctg ctgagcgagt tcgtgcccgg 2460
cgccggcttc gtgctgggcc tggtggacat catctggggc atcttcggcc ccagccagtg 2520
ggacgccttc ctggtgcaga tcgagcagtt gataaaccaa cgcatagagg aattcgcccg 2580
caaccaggcc atcagccgcc tggagggcct gagcaacctg taccaaatct acgccgagag 2640
cttccgcgag tgggaggccg accccaccaa ccccgccctg cgcgaggaga tgcgcatcca 2700
gttcaacgac atgaacagcg ccctgaccac cgccatcccc ctgttcgccg tgcagaacta 2760
ccaggtgccc ctgctgagcg tgtacgtgca ggccgccaac ctgcacctga gcgtgctgcg 2820
cgacgtcagc gtgttcggcc agcgctgggg cttcgacgcc gccaccatca acagccgcta 2880
caacgacctg acccgcctga tcggcaacta caccgaccac gccgtgcgct ggtacaacac 2940
cggcctggag cgcgtgtggg ggcccgacag ccgcgactgg atcaggtaca accagttccg 3000
ccgcgagctg accctgaccg tgctggacat cgtgagcctg ttccccaact acgacagccg 3060
cacctacccc atccgcaccg tgagccagct gacccgcgag atttacacca accccgtgct 3120
ggagaacttc gacggcagct tccgcggcag cgcccagggc atcgagggca gcatccgcag 3180
cccccacctg atggacatcc tgaacagcat caccatctac accgacgccc accgcggcga 3240
gtactactgg agcggccacc agatcatggc cagccccgtc ggcttcagcg gccccgagtt 3300
caccttcccc ctgtacggca cgatgggcaa cgctgcacct cagcagcgca tcgtggcaca 3360
gctgggccag ggagtgtacc gcaccctgag cagcaccctg taccgtcgac ctttcaacat 3420
cggcatcaac aaccagcagc tgagcgtgct ggacggcacc gagttcgcct acggcaccag 3480
cagcaacctg cccagcgccg tgtaccgcaa gagcggcacc gtggacagcc tggacgagat 3540
cccccctcag aacaacaacg tgccacctcg acagggcttc agccaccgtc tgagccacgt 3600
gagcatgttc cgcagtggct tcagcaacag cagcgtgagc atcatccgtg cacctatgtt 3660
cagctggatt caccgcagtg ccgagttcaa caacatcatc cccagcagcc agatcaccca 3720
gatccccctg accaagagca ccaacctggg cagcggcacc agcgtggtga agggccccgg 3780
cttcaccggc ggcgacatcc tgcgccgcac cagccccggc cagatcagca ccctgcgcgt 3840
gaacatcacc gcccccctga gccagcgcta ccgcgtccgc atccgctacg ccagcaccac 3900
caacctgcag ttccacacca gcatcgacgg ccgccccatc aaccagggca acttcagcgc 3960
caccatgagc agcggcagca acctgcagag cggcagcttc cgcaccgtgg gcttcaccac 4020
ccccttcaac ttcagcaacg gcagcagcgt gttcaccctg agcgcccacg tgttcaacag 4080
cggcaacgag gtgtacatcg accgcatcga gttcgtgccc gccgaggtga ccttcgaggc 4140
cgagtacgac ctggagaggg ctcagaaggc cgtgaacgag ctgttcacca gcagcaacca 4200
gatcggcctg aagaccgacg tgaccgacta ccacatcgac caggtgagca acctggtgga 4260
gtgcttaagc gacgagttct gcctggacga gaagaaggag ctgagcgaga aggtgaagca 4320
cgccaagcgc ctgagcgacg agcgcaacct gctgcaggac cccaacttcc gcggcatcaa 4380
ccgccagctg gaccgcggct ggcgaggcag caccgatatc accatccagg gcggcgacga 4440
cgtgttcaag gagaactacg tgaccctgct gggcaccttc gacgagtgct accccaccta 4500
cctgtaccag aagatcgacg agagcaagct gaaggcctac acccgctacc agctgcgcgg 4560
ctacatcgag gacagccagg acctggaaat ctacctgatc cgctacaacg cgaagcacga 4620
gaccgtgaac gtgcccggca ccggcagcct gtggcccctg agcgccccca gccccatcgg 4680
caagtgcact agtcaccaca gccaccactt cagcctggac atcgacgtgg gctgcaccga 4740
cctgaacgag gacctgggcg tgtgggtgat cttcaagatc aagacccagg acggccacgc 4800
ccgcctgggc aatctagagt tcctggagga gaagcccctg gtgggcgagg ccctggcccg 4860
cgtgaagcgt gctgagaaga agtggcgcga caagcgcgag aagctggagt gggagaccaa 4920
catcgtgtac aaggaggcca aggagagcgt ggacgccctg ttcgtgaaca gccagtacga 4980
ccgcctgcag gccgacacca acatcgccat gatccacgcc gccgacaagc gcgtgcacag 5040
cattcgcgag gcctacctgc ccgagctgag cgtgatcccc ggtgtgaacg ccgccatctt 5100
cgaggaactc gagggccgca tctaggagct ctatgatcgt tcaaacattt ggcaataaag 5160
tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa 5220
ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt 5280
tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc 5340
aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcaat agactacgga 5400
ccgcgatcgc ttaattaagc ttgcatgcct gcagtgcagc gtgacccggt cgtgcccctc 5460
tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt 5520
cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg 5580
aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac 5640
agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc 5700
tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc 5760
atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt 5820
ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt 5880
ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt 5940
aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag 6000
ataatgccag cctgttaaac gccgccgacg agtctaacgg acaccaacca gcgaaccagc 6060
agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc 6120
cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg 6180
tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacc 6240
ggcagctacg ggggattcct ttcccaccgc tccttcgctt tcccttcctc gcccgccgta 6300
ataaatagac accccctcca caccctcttt ccccaacctc gtgttgttcg gagcgcacac 6360
acacacaacc agatctcccc caaatccacc cgtcggcacc tccgcttcaa ggtacgccgc 6420
tcgtcctccc cccccccccc tctctacctt ctctagatcg gcgttccggt ccatagttag 6480
ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt 6540
gctgttagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga ttgctaactt 6600
gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag acgggatcga 6660
tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc tttatttcaa 6720
tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt cttggttgtg 6780
atgatgtggt ctggttgggc ggtcgttcta gatcggagta gaattctgtt tcaaactacc 6840
tggtggattt attaattttg gatctgtatg tgtgtgccat acatattcat agttacgaat 6900
tgaagatgat ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac 6960
tgatgcatat acagagatgc tttttgttcg cttggttgtg atgatgtggt gtggttgggc 7020
ggtcgttcat tcgttctaga tcggagtaga atactgtttc aaactacctg gtgtatttat 7080
taattttgga actgtatgtg tgtgtcatac atcttcatag ttacgagttt aagatggatg 7140
gaaatatcga tctaggatag gtatacatgt tgatgtgggt tttactgatg catatacatg 7200
atggcatatg cagcatctat tcatatgctc taaccttgag tacctatcta ttataataaa 7260
caagtatgtt ttataattat tttgatcttg atatacttgg atgatggcat atgcagcagc 7320
tatatgtgga tttttttagc cctgccttca tacgctattt atttgcttgg tactgtttct 7380
tttgtcgatg ctcaccctgt tgtttggtgt tacttctgca gggatctccg atcatgcaaa 7440
aactcattaa ctcagtgcaa aactatgcct ggggcagcaa aacggcgttg actgaacttt 7500
atggtatgga aaatccgtcc agccagccga tggccgagct gtggatgggc gcacatccga 7560
aaagcagttc acgagtgcag aatgccgccg gagatatcgt ttcactgcgt gatgtgattg 7620
agagtgataa atcgactctg ctcggagagg ccgttgccaa acgctttggc gaactgcctt 7680
tcctgttcaa agtattatgc gcagcacagc cactctccat tcaggttcat ccaaacaaac 7740
acaattctga aatcggtttt gccaaagaaa atgccgcagg tatcccgatg gatgccgccg 7800
agcgtaacta taaagatcct aaccacaagc cggagctggt ttttgcgctg acgcctttcc 7860
ttgcgatgaa cgcgtttcgt gaattttccg agattgtctc cctactccag ccggtcgcag 7920
gtgcacatcc ggcgattgct cactttttac aacagcctga tgccgaacgt ttaagcgaac 7980
tgttcgccag cctgttgaat atgcagggtg aagaaaaatc ccgcgcgctg gcgattttaa 8040
aatcggccct cgatagccag cagggtgaac cgtggcaaac gattcgttta atttctgaat 8100
tttacccgga agacagcggt ctgttctccc cgctattgct gaatgtggtg aaattgaacc 8160
ctggcgaagc gatgttcctg ttcgctgaaa caccgcacgc ttacctgcaa ggcgtggcgc 8220
tggaagtgat ggcaaactcc gataacgtgc tgcgtgcggg tctgacgcct aaatacattg 8280
atattccgga actggttgcc aatgtgaaat tcgaagccaa accggctaac cagttgttga 8340
cccagccggt gaaacaaggt gcagaactgg acttcccgat tccagtggat gattttgcct 8400
tctcgctgca tgaccttagt gataaagaaa ccaccattag ccagcagagt gccgccattt 8460
tgttctgcgt cgaaggcgat gcaacgttgt ggaaaggttc tcagcagtta cagcttaaac 8520
cgggtgaatc agcgtttatt gccgccaacg aatcaccggt gactgtcaaa ggccacggcc 8580
gtttagcgcg tgtttacaac aagctgtaag agcttactga aaaaattaac atctcttgct 8640
aagctgggtc atgggtcgtt taagctgccg atgtgcctgc gtcgtctggt gccctctctc 8700
catatggagg ttgtcaaagt atctgctgtt cgtgtcatga gtcgtgtcag tgttggttta 8760
ataatggacc ggttgtgttg tgtgtgcgta ctacccagaa ctatgacaaa tcatgaataa 8820
gtttgatgtt tgaaattaaa gcctgtgctc attatgttct gtctttcagt tgtctcctaa 8880
tatttgcctc caggtactgg ctatctaccg tttcttactt aggaggtgtt tgaatgcact 8940
aaaactaata gttagtggct aaaattagtt aaaacatcca aacaccatag ctaatagttg 9000
aactattagc tatttttgga aaattagtta atagtgaggt agttatttgt tagctagcta 9060
attcaactaa caatttttag ccaactaaca attagtttca gtgcattcaa acaccccctt 9120
aatgttaacg tggttctatc taccgtctcc taatatatgg ttgattgttc ggtttgttgc 9180
tatgctattg ggttctgatt gctgctagtt cttgctgaat ccagaagttc tcgtagtata 9240
gctcagattc atattattta tttgagtgat aagtgatcca ggttattact atgttagcta 9300
ggtttttttt acaaggataa attatctgtg atcataattc ttatgaaagc tttatgtttc 9360
ctggaggcag tggcatgcaa tgcatgacag caacttgatc acaccagctg aggtagatac 9420
ggtaacaagg ttcttaaatc tgttcaccaa atcattggag aacacacata cacattcttg 9480
ccagtcttgg ttagagaaat ttcatgacaa aatgccaaag ctgtcttgac tcttcacttt 9540
tggccatgag tcgtgactta gtttggttta atggaccggt tctcctagct tgttctactc 9600
aaaactgttg ttgatgcgaa taagttgtga tggttgatct ctggattttg ttttgctctc 9660
aatagtggac gagattagat agcctgcagg cccgggggcg cgccctaatt agctaacggc 9720
caggatcgcc gcgtgagcct ttagcaacta gctagattaa ttaacgcaat ctgttattaa 9780
gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc 9840
tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca tcagaattaa 9900
ttctcatgtt tgacagctta tcatcgactg cacggtgcac caatgcttct ggcgtcaggc 9960
agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc 10020
tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca 10080
aatattctga aatgagctgt tgacaattaa tcatccggct cgtataatgt gtggaattgt 10140
gagcggataa caatttcaca caggaaacag accatgaggg aagcgttgat cgccgaagta 10200
tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg 10260
gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat 10320
ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac 10380
cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc 10440
attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt 10500
ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt 10560
gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg 10620
gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc 10680
ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg 10740
ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc 10800
gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctaggcag 10860
gcttatcttg gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt 10920
cactacgtga aaggcgagat caccaaagta gtcggcaaat aaagctctag tggatctccg 10980
tacccaggga tctggctcgc ggcggacgca cgacgccggg gcgagaccat aggcgatctc 11040
ctaaatcaat agtagctgta acctcgaagc gtttcacttg taacaacgat tgagaatttt 11100
tgtcataaaa ttgaaatact tggttcgcat ttttgtcatc cgcggtcagc cgcaattctg 11160
acgaactgcc catttagctg gagatgattg tacatccttc acgtgaaaat ttctcaagcg 11220
ctgtgaacaa gggttcagat tttagattga aaggtgagcc gttgaaacac gttcttcttg 11280
tcgatgacga cgtcgctatg cggcatctta ttattgaata ccttacgatc cacgccttca 11340
aagtgaccgc ggtagccgac agcacccagt tcacaagagt actctcttcc gcgacggtcg 11400
atgtcgtggt tgttgatcta gatttaggtc gtgaagatgg gctcgagatc gttcgtaatc 11460
tggcggcaaa gtctgatatt ccaatcataa ttatcagtgg cgaccgcctt gaggagacgg 11520
ataaagttgt tgcactcgag ctaggagcaa gtgattttat cgctaagccg ttcagtatca 11580
gagagtttct agcacgcatt cgggttgcct tgcgcgtgcg ccccaacgtt gtccgctcca 11640
aagaccgacg gtctttttgt tttactgact ggacacttaa tctcaggcaa cgtcgcttga 11700
tgtccgaagc tggcggtgag gtgaaactta cggcaggtga gttcaatctt ctcctcgcgt 11760
ttttagagaa accccgcgac gttctatcgc gcgagcaact tctcattgcc agtcgagtac 11820
gcgacgagga ggtttatgac aggagtatag atgttctcat tttgaggctg cgccgcaaac 11880
ttgaggcaga tccgtcaagc cctcaactga taaaaacagc aagaggtgcc ggttatttct 11940
ttgacgcgga cgtgcaggtt tcgcacgggg ggacgatggc agcctgagcc aattcccaga 12000
tccccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc 12060
gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat 12120
cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga 12180
atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga 12240
gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat 12300
catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg 12360
ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt 12420
gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata 12480
ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact 12540
caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat 12600
tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct 12660
ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac 12720
cgggcggccg gagtacatcg agatcgagct ggctgattgg atgtaccgcg agatcacaga 12780
aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat 12840
cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt 12900
gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac 12960
cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg 13020
gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc 13080
cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg 13140
aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa 13200
ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt 13260
gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa 13320
aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct 13380
gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc 13440
tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg 13500
cggacaagcc gcgccgtcgc cactcgaccg ccggcgctga ggtctgcctc gtgaagaagg 13560
tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag tgagggagcc 13620
acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact tttgctttgc 13680
cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact cagcaaaagt 13740
tcgatttatt caacaaagcc gccgtcccgt caagtcagcg taatgctctg ccagtgttac 13800
aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta 13860
ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa 13920
aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact 13980
cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag 14040
aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagctctgc attaatgaat 14100
cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 14160
tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 14220
aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 14280
gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 14340
ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 14400
ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 14460
gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 14520
ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 14580
cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 14640
cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 14700
gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 14760
aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 14820
tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 14880
gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 14940
tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 15000
gatcttcacc tagatccttt tgatccggaa tta 15033
<210> 54
<211> 15032
<212> DNA
<213> 人工序列
<220>
<223> 载体24380
<400> 54
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctagacat ccaacacgca cgagatgaca gatagagcag tgagctgacc 1260
atctcttact aggtacgccg ctcgtcctcc cccccccccc ctctctacct tctctagatc 1320
ggcgttccgg tccatagtta gggcccggta gttctacttc tgttcatgtt tgtgttagat 1380
ccgtgtttgt gttagatccg tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag 1440
acacgttctg attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag 1500
ccgttccgca gacgggatcg atttcatgat tttttttgtt tcgttgcata gggtttggtt 1560
tgcccttttc ctttatttca atatatgccg tgcacttgtt tgtcgggtca tcttttcatg 1620
cttttttttg tcttggttgt gatgatgtgg tctggttggg cggtcgttct agatcggagt 1680
agaattctgt ttcaaactac ctggtggatt tattaatttt ggatctgtat gtgtgtgcca 1740
tacatattca tagttacgaa ttgaagatga tggatggaaa tatcgatcta ggataggtat 1800
acatgttgat gcgggtttta ctgatgcata tacagagatg ctttttgttc gcttggttgt 1860
gatgatgtgg tgtggttggg cggtcgttca ttcgttctag atcggagtag aatactgttt 1920
caaactacct ggtgtattta ttaattttgg aactgtatgt gtgtgtcata catcttcata 1980
gttacgagtt taagatggat ggaaatatcg atctaggata ggtatacatg ttgatgtggg 2040
ttttactgat gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga 2100
gtacctatct attataataa acaagtatgt tttataatta ttttgatctt gatatacttg 2160
gatgatggca tatccagcag ctatatgtgg atttttttag ccctgccttc atacgctatt 2220
tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc 2280
aggatcctct aagtagcagc catggacaac aaccccaaca tcaacgagtg catcccctac 2340
aactgcctga gcaaccccga ggtggaggtg ctgggcggcg agcgcatcga gaccggctac 2400
acccccatcg acatcagcct gagcctgacc cagttcctgc tgagcgagtt cgtgcccggc 2460
gccggcttcg tgctgggcct ggtggacatc atctggggca tcttcggccc cagccagtgg 2520
gacgccttcc tggtgcagat cgagcagttg ataaaccaac gcatagagga attcgcccgc 2580
aaccaggcca tcagccgcct ggagggcctg agcaacctgt accaaatcta cgccgagagc 2640
ttccgcgagt gggaggccga ccccaccaac cccgccctgc gcgaggagat gcgcatccag 2700
ttcaacgaca tgaacagcgc cctgaccacc gccatccccc tgttcgccgt gcagaactac 2760
caggtgcccc tgctgagcgt gtacgtgcag gccgccaacc tgcacctgag cgtgctgcgc 2820
gacgtcagcg tgttcggcca gcgctggggc ttcgacgccg ccaccatcaa cagccgctac 2880
aacgacctga cccgcctgat cggcaactac accgaccacg ccgtgcgctg gtacaacacc 2940
ggcctggagc gcgtgtgggg gcccgacagc cgcgactgga tcaggtacaa ccagttccgc 3000
cgcgagctga ccctgaccgt gctggacatc gtgagcctgt tccccaacta cgacagccgc 3060
acctacccca tccgcaccgt gagccagctg acccgcgaga tttacaccaa ccccgtgctg 3120
gagaacttcg acggcagctt ccgcggcagc gcccagggca tcgagggcag catccgcagc 3180
ccccacctga tggacatcct gaacagcatc accatctaca ccgacgccca ccgcggcgag 3240
tactactgga gcggccacca gatcatggcc agccccgtcg gcttcagcgg ccccgagttc 3300
accttccccc tgtacggcac gatgggcaac gctgcacctc agcagcgcat cgtggcacag 3360
ctgggccagg gagtgtaccg caccctgagc agcaccctgt accgtcgacc tttcaacatc 3420
ggcatcaaca accagcagct gagcgtgctg gacggcaccg agttcgccta cggcaccagc 3480
agcaacctgc ccagcgccgt gtaccgcaag agcggcaccg tggacagcct ggacgagatc 3540
ccccctcaga acaacaacgt gccacctcga cagggcttca gccaccgtct gagccacgtg 3600
agcatgttcc gcagtggctt cagcaacagc agcgtgagca tcatccgtgc acctatgttc 3660
agctggattc accgcagtgc cgagttcaac aacatcatcc ccagcagcca gatcacccag 3720
atccccctga ccaagagcac caacctgggc agcggcacca gcgtggtgaa gggccccggc 3780
ttcaccggcg gcgacatcct gcgccgcacc agccccggcc agatcagcac cctgcgcgtg 3840
aacatcaccg cccccctgag ccagcgctac cgcgtccgca tccgctacgc cagcaccacc 3900
aacctgcagt tccacaccag catcgacggc cgccccatca accagggcaa cttcagcgcc 3960
accatgagca gcggcagcaa cctgcagagc ggcagcttcc gcaccgtggg cttcaccacc 4020
cccttcaact tcagcaacgg cagcagcgtg ttcaccctga gcgcccacgt gttcaacagc 4080
ggcaacgagg tgtacatcga ccgcatcgag ttcgtgcccg ccgaggtgac cttcgaggcc 4140
gagtacgacc tggagagggc tcagaaggcc gtgaacgagc tgttcaccag cagcaaccag 4200
atcggcctga agaccgacgt gaccgactac cacatcgacc aggtgagcaa cctggtggag 4260
tgcttaagcg acgagttctg cctggacgag aagaaggagc tgagcgagaa ggtgaagcac 4320
gccaagcgcc tgagcgacga gcgcaacctg ctgcaggacc ccaacttccg cggcatcaac 4380
cgccagctgg accgcggctg gcgaggcagc accgatatca ccatccaggg cggcgacgac 4440
gtgttcaagg agaactacgt gaccctgctg ggcaccttcg acgagtgcta ccccacctac 4500
ctgtaccaga agatcgacga gagcaagctg aaggcctaca cccgctacca gctgcgcggc 4560
tacatcgagg acagccagga cctggaaatc tacctgatcc gctacaacgc gaagcacgag 4620
accgtgaacg tgcccggcac cggcagcctg tggcccctga gcgcccccag ccccatcggc 4680
aagtgcacta gtcaccacag ccaccacttc agcctggaca tcgacgtggg ctgcaccgac 4740
ctgaacgagg acctgggcgt gtgggtgatc ttcaagatca agacccagga cggccacgcc 4800
cgcctgggca atctagagtt cctggaggag aagcccctgg tgggcgaggc cctggcccgc 4860
gtgaagcgtg ctgagaagaa gtggcgcgac aagcgcgaga agctggagtg ggagaccaac 4920
atcgtgtaca aggaggccaa ggagagcgtg gacgccctgt tcgtgaacag ccagtacgac 4980
cgcctgcagg ccgacaccaa catcgccatg atccacgccg ccgacaagcg cgtgcacagc 5040
attcgcgagg cctacctgcc cgagctgagc gtgatccccg gtgtgaacgc cgccatcttc 5100
gaggaactcg agggccgcat ctaggagctc tatgatcgtt caaacatttg gcaataaagt 5160
ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat 5220
tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt 5280
atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca 5340
aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcaata gactacggac 5400
cgcgatcgct taattaagct tgcatgcctg cagtgcagcg tgacccggtc gtgcccctct 5460
ctagagataa tgagcattgc atgtctaagt tataaaaaat taccacatat tttttttgtc 5520
acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt tactctacga 5580
ataatataat ctatagtact acaataatat cagtgtttta gagaatcata taaatgaaca 5640
gttagacatg gtctaaagga caattgagta ttttgacaac aggactctac agttttatct 5700
ttttagtgtg catgtgttct cctttttttt tgcaaatagc ttcacctata taatacttca 5760
tccattttat tagtacatcc atttagggtt tagggttaat ggtttttata gactaatttt 5820
tttagtacat ctattttatt ctattttagc ctctaaatta agaaaactaa aactctattt 5880
tagttttttt atttaataat ttagatataa aatagaataa aataaagtga ctaaaaatta 5940
aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga 6000
taatgccagc ctgttaaacg ccgccgacga gtctaacgga caccaaccag cgaaccagca 6060
gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct ctgtcgctgc ctctggaccc 6120
ctctcgagag ttccgctcca ccgttggact tgctccgctg tcggcatcca gaaattgcgt 6180
ggcggagcgg cagacgtgag ccggcacggc aggcggcctc ctcctcctct cacggcaccg 6240
gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa 6300
taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca 6360
cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct 6420
cgtcctcccc ccccccccct ctctaccttc tctagatcgg cgttccggtc catagttagg 6480
gcccggtagt tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg 6540
ctgttagcgt tcgtacacgg atgcgacctg tacgtcagac acgttctgat tgctaacttg 6600
ccagtgtttc tctttgggga atcctgggat ggctctagcc gttccgcaga cgggatcgat 6660
ttcatgattt tttttgtttc gttgcatagg gtttggtttg cccttttcct ttatttcaat 6720
atatgccgtg cacttgtttg tcgggtcatc ttttcatgct tttttttgtc ttggttgtga 6780
tgatgtggtc tggttgggcg gtcgttctag atcggagtag aattctgttt caaactacct 6840
ggtggattta ttaattttgg atctgtatgt gtgtgccata catattcata gttacgaatt 6900
gaagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc gggttttact 6960
gatgcatata cagagatgct ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg 7020
gtcgttcatt cgttctagat cggagtagaa tactgtttca aactacctgg tgtatttatt 7080
aattttggaa ctgtatgtgt gtgtcataca tcttcatagt tacgagttta agatggatgg 7140
aaatatcgat ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga 7200
tggcatatgc agcatctatt catatgctct aaccttgagt acctatctat tataataaac 7260
aagtatgttt tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct 7320
atatgtggat ttttttagcc ctgccttcat acgctattta tttgcttggt actgtttctt 7380
ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag ggatctccga tcatgcaaaa 7440
actcattaac tcagtgcaaa actatgcctg gggcagcaaa acggcgttga ctgaacttta 7500
tggtatggaa aatccgtcca gccagccgat ggccgagctg tggatgggcg cacatccgaa 7560
aagcagttca cgagtgcaga atgccgccgg agatatcgtt tcactgcgtg atgtgattga 7620
gagtgataaa tcgactctgc tcggagaggc cgttgccaaa cgctttggcg aactgccttt 7680
cctgttcaaa gtattatgcg cagcacagcc actctccatt caggttcatc caaacaaaca 7740
caattctgaa atcggttttg ccaaagaaaa tgccgcaggt atcccgatgg atgccgccga 7800
gcgtaactat aaagatccta accacaagcc ggagctggtt tttgcgctga cgcctttcct 7860
tgcgatgaac gcgtttcgtg aattttccga gattgtctcc ctactccagc cggtcgcagg 7920
tgcacatccg gcgattgctc actttttaca acagcctgat gccgaacgtt taagcgaact 7980
gttcgccagc ctgttgaata tgcagggtga agaaaaatcc cgcgcgctgg cgattttaaa 8040
atcggccctc gatagccagc agggtgaacc gtggcaaacg attcgtttaa tttctgaatt 8100
ttacccggaa gacagcggtc tgttctcccc gctattgctg aatgtggtga aattgaaccc 8160
tggcgaagcg atgttcctgt tcgctgaaac accgcacgct tacctgcaag gcgtggcgct 8220
ggaagtgatg gcaaactccg ataacgtgct gcgtgcgggt ctgacgccta aatacattga 8280
tattccggaa ctggttgcca atgtgaaatt cgaagccaaa ccggctaacc agttgttgac 8340
ccagccggtg aaacaaggtg cagaactgga cttcccgatt ccagtggatg attttgcctt 8400
ctcgctgcat gaccttagtg ataaagaaac caccattagc cagcagagtg ccgccatttt 8460
gttctgcgtc gaaggcgatg caacgttgtg gaaaggttct cagcagttac agcttaaacc 8520
gggtgaatca gcgtttattg ccgccaacga atcaccggtg actgtcaaag gccacggccg 8580
tttagcgcgt gtttacaaca agctgtaaga gcttactgaa aaaattaaca tctcttgcta 8640
agctgggtca tgggtcgttt aagctgccga tgtgcctgcg tcgtctggtg ccctctctcc 8700
atatggaggt tgtcaaagta tctgctgttc gtgtcatgag tcgtgtcagt gttggtttaa 8760
taatggaccg gttgtgttgt gtgtgcgtac tacccagaac tatgacaaat catgaataag 8820
tttgatgttt gaaattaaag cctgtgctca ttatgttctg tctttcagtt gtctcctaat 8880
atttgcctcc aggtactggc tatctaccgt ttcttactta ggaggtgttt gaatgcacta 8940
aaactaatag ttagtggcta aaattagtta aaacatccaa acaccatagc taatagttga 9000
actattagct atttttggaa aattagttaa tagtgaggta gttatttgtt agctagctaa 9060
ttcaactaac aatttttagc caactaacaa ttagtttcag tgcattcaaa caccccctta 9120
atgttaacgt ggttctatct accgtctcct aatatatggt tgattgttcg gtttgttgct 9180
atgctattgg gttctgattg ctgctagttc ttgctgaatc cagaagttct cgtagtatag 9240
ctcagattca tattatttat ttgagtgata agtgatccag gttattacta tgttagctag 9300
gtttttttta caaggataaa ttatctgtga tcataattct tatgaaagct ttatgtttcc 9360
tggaggcagt ggcatgcaat gcatgacagc aacttgatca caccagctga ggtagatacg 9420
gtaacaaggt tcttaaatct gttcaccaaa tcattggaga acacacatac acattcttgc 9480
cagtcttggt tagagaaatt tcatgacaaa atgccaaagc tgtcttgact cttcactttt 9540
ggccatgagt cgtgacttag tttggtttaa tggaccggtt ctcctagctt gttctactca 9600
aaactgttgt tgatgcgaat aagttgtgat ggttgatctc tggattttgt tttgctctca 9660
atagtggacg agattagata gcctgcaggc ccgggggcgc gccctaatta gctaacggcc 9720
aggatcgccg cgtgagcctt tagcaactag ctagattaat taacgcaatc tgttattaag 9780
ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct 9840
ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagaattaat 9900
tctcatgttt gacagcttat catcgactgc acggtgcacc aatgcttctg gcgtcaggca 9960
gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcataat tcgtgtcgct 10020
caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa 10080
atattctgaa atgagctgtt gacaattaat catccggctc gtataatgtg tggaattgtg 10140
agcggataac aatttcacac aggaaacaga ccatgaggga agcgttgatc gccgaagtat 10200
cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg 10260
ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt 10320
tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc 10380
ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca 10440
ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg 10500
gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg 10560
atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg 10620
cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct 10680
taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt 10740
tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg 10800
actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctaggcagg 10860
cttatcttgg acaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgttc 10920
actacgtgaa aggcgagatc accaaagtag tcggcaaata aagctctagt ggatctccgt 10980
acccagggat ctggctcgcg gcggacgcac gacgccgggg cgagaccata ggcgatctcc 11040
taaatcaata gtagctgtaa cctcgaagcg tttcacttgt aacaacgatt gagaattttt 11100
gtcataaaat tgaaatactt ggttcgcatt tttgtcatcc gcggtcagcc gcaattctga 11160
cgaactgccc atttagctgg agatgattgt acatccttca cgtgaaaatt tctcaagcgc 11220
tgtgaacaag ggttcagatt ttagattgaa aggtgagccg ttgaaacacg ttcttcttgt 11280
cgatgacgac gtcgctatgc ggcatcttat tattgaatac cttacgatcc acgccttcaa 11340
agtgaccgcg gtagccgaca gcacccagtt cacaagagta ctctcttccg cgacggtcga 11400
tgtcgtggtt gttgatctag atttaggtcg tgaagatggg ctcgagatcg ttcgtaatct 11460
ggcggcaaag tctgatattc caatcataat tatcagtggc gaccgccttg aggagacgga 11520
taaagttgtt gcactcgagc taggagcaag tgattttatc gctaagccgt tcagtatcag 11580
agagtttcta gcacgcattc gggttgcctt gcgcgtgcgc cccaacgttg tccgctccaa 11640
agaccgacgg tctttttgtt ttactgactg gacacttaat ctcaggcaac gtcgcttgat 11700
gtccgaagct ggcggtgagg tgaaacttac ggcaggtgag ttcaatcttc tcctcgcgtt 11760
tttagagaaa ccccgcgacg ttctatcgcg cgagcaactt ctcattgcca gtcgagtacg 11820
cgacgaggag gtttatgaca ggagtataga tgttctcatt ttgaggctgc gccgcaaact 11880
tgaggcagat ccgtcaagcc ctcaactgat aaaaacagca agaggtgccg gttatttctt 11940
tgacgcggac gtgcaggttt cgcacggggg gacgatggca gcctgagcca attcccagat 12000
ccccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg 12060
ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc 12120
gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa 12180
tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag 12240
caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc 12300
atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc 12360
tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg 12420
tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac 12480
cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc 12540
aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt 12600
cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg 12660
gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc 12720
gggcggccgg agtacatcga gatcgagctg gctgattgga tgtaccgcga gatcacagaa 12780
ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc 12840
ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg 12900
ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc 12960
gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg 13020
caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc 13080
ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga 13140
aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac 13200
cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg 13260
actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa 13320
actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg 13380
caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct 13440
atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc 13500
ggacaagccg cgccgtcgcc actcgaccgc cggcgctgag gtctgcctcg tgaagaaggt 13560
gttgctgact cataccaggc ctgaatcgcc ccatcatcca gccagaaagt gagggagcca 13620
cggttgatga gagctttgtt gtaggtggac cagttggtga ttttgaactt ttgctttgcc 13680
acggaacggt ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc agcaaaagtt 13740
cgatttattc aacaaagccg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca 13800
accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat 13860
tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 13920
actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 13980
gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 14040
aatcaccatg agtgacgact gaatccggtg agaatggcaa aagctctgca ttaatgaatc 14100
ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 14160
gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 14220
atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 14280
caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 14340
cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 14400
taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 14460
ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 14520
tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 14580
gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 14640
ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 14700
aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 14760
agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 14820
agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 14880
cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 14940
gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 15000
atcttcacct agatcctttt gatccggaat ta 15032
<210> 55
<211> 15030
<212> DNA
<213> 人工序列
<220>
<223> 载体24381
<400> 55
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctagcatc tctgttgcct ttttgattga accgtgccaa cagcttgttg 1260
gatttactag gtacgccgct cgtcctcccc ccccccccct ctctaccttc tctagatcgg 1320
cgttccggtc catagttagg gcccggtagt tctacttctg ttcatgtttg tgttagatcc 1380
gtgtttgtgt tagatccgtg ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac 1440
acgttctgat tgctaacttg ccagtgtttc tctttgggga atcctgggat ggctctagcc 1500
gttccgcaga cgggatcgat ttcatgattt tttttgtttc gttgcatagg gtttggtttg 1560
cccttttcct ttatttcaat atatgccgtg cacttgtttg tcgggtcatc ttttcatgct 1620
tttttttgtc ttggttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag 1680
aattctgttt caaactacct ggtggattta ttaattttgg atctgtatgt gtgtgccata 1740
catattcata gttacgaatt gaagatgatg gatggaaata tcgatctagg ataggtatac 1800
atgttgatgc gggttttact gatgcatata cagagatgct ttttgttcgc ttggttgtga 1860
tgatgtggtg tggttgggcg gtcgttcatt cgttctagat cggagtagaa tactgtttca 1920
aactacctgg tgtatttatt aattttggaa ctgtatgtgt gtgtcataca tcttcatagt 1980
tacgagttta agatggatgg aaatatcgat ctaggatagg tatacatgtt gatgtgggtt 2040
ttactgatgc atatacatga tggcatatgc agcatctatt catatgctct aaccttgagt 2100
acctatctat tataataaac aagtatgttt tataattatt ttgatcttga tatacttgga 2160
tgatggcata tccagcagct atatgtggat ttttttagcc ctgccttcat acgctattta 2220
tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag 2280
gatcctctaa gtagcagcca tggacaacaa ccccaacatc aacgagtgca tcccctacaa 2340
ctgcctgagc aaccccgagg tggaggtgct gggcggcgag cgcatcgaga ccggctacac 2400
ccccatcgac atcagcctga gcctgaccca gttcctgctg agcgagttcg tgcccggcgc 2460
cggcttcgtg ctgggcctgg tggacatcat ctggggcatc ttcggcccca gccagtggga 2520
cgccttcctg gtgcagatcg agcagttgat aaaccaacgc atagaggaat tcgcccgcaa 2580
ccaggccatc agccgcctgg agggcctgag caacctgtac caaatctacg ccgagagctt 2640
ccgcgagtgg gaggccgacc ccaccaaccc cgccctgcgc gaggagatgc gcatccagtt 2700
caacgacatg aacagcgccc tgaccaccgc catccccctg ttcgccgtgc agaactacca 2760
ggtgcccctg ctgagcgtgt acgtgcaggc cgccaacctg cacctgagcg tgctgcgcga 2820
cgtcagcgtg ttcggccagc gctggggctt cgacgccgcc accatcaaca gccgctacaa 2880
cgacctgacc cgcctgatcg gcaactacac cgaccacgcc gtgcgctggt acaacaccgg 2940
cctggagcgc gtgtgggggc ccgacagccg cgactggatc aggtacaacc agttccgccg 3000
cgagctgacc ctgaccgtgc tggacatcgt gagcctgttc cccaactacg acagccgcac 3060
ctaccccatc cgcaccgtga gccagctgac ccgcgagatt tacaccaacc ccgtgctgga 3120
gaacttcgac ggcagcttcc gcggcagcgc ccagggcatc gagggcagca tccgcagccc 3180
ccacctgatg gacatcctga acagcatcac catctacacc gacgcccacc gcggcgagta 3240
ctactggagc ggccaccaga tcatggccag ccccgtcggc ttcagcggcc ccgagttcac 3300
cttccccctg tacggcacga tgggcaacgc tgcacctcag cagcgcatcg tggcacagct 3360
gggccaggga gtgtaccgca ccctgagcag caccctgtac cgtcgacctt tcaacatcgg 3420
catcaacaac cagcagctga gcgtgctgga cggcaccgag ttcgcctacg gcaccagcag 3480
caacctgccc agcgccgtgt accgcaagag cggcaccgtg gacagcctgg acgagatccc 3540
ccctcagaac aacaacgtgc cacctcgaca gggcttcagc caccgtctga gccacgtgag 3600
catgttccgc agtggcttca gcaacagcag cgtgagcatc atccgtgcac ctatgttcag 3660
ctggattcac cgcagtgccg agttcaacaa catcatcccc agcagccaga tcacccagat 3720
ccccctgacc aagagcacca acctgggcag cggcaccagc gtggtgaagg gccccggctt 3780
caccggcggc gacatcctgc gccgcaccag ccccggccag atcagcaccc tgcgcgtgaa 3840
catcaccgcc cccctgagcc agcgctaccg cgtccgcatc cgctacgcca gcaccaccaa 3900
cctgcagttc cacaccagca tcgacggccg ccccatcaac cagggcaact tcagcgccac 3960
catgagcagc ggcagcaacc tgcagagcgg cagcttccgc accgtgggct tcaccacccc 4020
cttcaacttc agcaacggca gcagcgtgtt caccctgagc gcccacgtgt tcaacagcgg 4080
caacgaggtg tacatcgacc gcatcgagtt cgtgcccgcc gaggtgacct tcgaggccga 4140
gtacgacctg gagagggctc agaaggccgt gaacgagctg ttcaccagca gcaaccagat 4200
cggcctgaag accgacgtga ccgactacca catcgaccag gtgagcaacc tggtggagtg 4260
cttaagcgac gagttctgcc tggacgagaa gaaggagctg agcgagaagg tgaagcacgc 4320
caagcgcctg agcgacgagc gcaacctgct gcaggacccc aacttccgcg gcatcaaccg 4380
ccagctggac cgcggctggc gaggcagcac cgatatcacc atccagggcg gcgacgacgt 4440
gttcaaggag aactacgtga ccctgctggg caccttcgac gagtgctacc ccacctacct 4500
gtaccagaag atcgacgaga gcaagctgaa ggcctacacc cgctaccagc tgcgcggcta 4560
catcgaggac agccaggacc tggaaatcta cctgatccgc tacaacgcga agcacgagac 4620
cgtgaacgtg cccggcaccg gcagcctgtg gcccctgagc gcccccagcc ccatcggcaa 4680
gtgcactagt caccacagcc accacttcag cctggacatc gacgtgggct gcaccgacct 4740
gaacgaggac ctgggcgtgt gggtgatctt caagatcaag acccaggacg gccacgcccg 4800
cctgggcaat ctagagttcc tggaggagaa gcccctggtg ggcgaggccc tggcccgcgt 4860
gaagcgtgct gagaagaagt ggcgcgacaa gcgcgagaag ctggagtggg agaccaacat 4920
cgtgtacaag gaggccaagg agagcgtgga cgccctgttc gtgaacagcc agtacgaccg 4980
cctgcaggcc gacaccaaca tcgccatgat ccacgccgcc gacaagcgcg tgcacagcat 5040
tcgcgaggcc tacctgcccg agctgagcgt gatccccggt gtgaacgccg ccatcttcga 5100
ggaactcgag ggccgcatct aggagctcta tgatcgttca aacatttggc aataaagttt 5160
cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta 5220
cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat 5280
gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa 5340
ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcaataga ctacggaccg 5400
cgatcgctta attaagcttg catgcctgca gtgcagcgtg acccggtcgt gcccctctct 5460
agagataatg agcattgcat gtctaagtta taaaaaatta ccacatattt tttttgtcac 5520
acttgtttga agtgcagttt atctatcttt atacatatat ttaaacttta ctctacgaat 5580
aatataatct atagtactac aataatatca gtgttttaga gaatcatata aatgaacagt 5640
tagacatggt ctaaaggaca attgagtatt ttgacaacag gactctacag ttttatcttt 5700
ttagtgtgca tgtgttctcc tttttttttg caaatagctt cacctatata atacttcatc 5760
cattttatta gtacatccat ttagggttta gggttaatgg tttttataga ctaatttttt 5820
tagtacatct attttattct attttagcct ctaaattaag aaaactaaaa ctctatttta 5880
gtttttttat ttaataattt agatataaaa tagaataaaa taaagtgact aaaaattaaa 5940
caaataccct ttaagaaatt aaaaaaacta aggaaacatt tttcttgttt cgagtagata 6000
atgccagcct gttaaacgcc gccgacgagt ctaacggaca ccaaccagcg aaccagcagc 6060
gtcgcgtcgg gccaagcgaa gcagacggca cggcatctct gtcgctgcct ctggacccct 6120
ctcgagagtt ccgctccacc gttggacttg ctccgctgtc ggcatccaga aattgcgtgg 6180
cggagcggca gacgtgagcc ggcacggcag gcggcctcct cctcctctca cggcaccggc 6240
agctacgggg gattcctttc ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata 6300
aatagacacc ccctccacac cctctttccc caacctcgtg ttgttcggag cgcacacaca 6360
cacaaccaga tctcccccaa atccacccgt cggcacctcc gcttcaaggt acgccgctcg 6420
tcctcccccc ccccccctct ctaccttctc tagatcggcg ttccggtcca tagttagggc 6480
ccggtagttc tacttctgtt catgtttgtg ttagatccgt gtttgtgtta gatccgtgct 6540
gttagcgttc gtacacggat gcgacctgta cgtcagacac gttctgattg ctaacttgcc 6600
agtgtttctc tttggggaat cctgggatgg ctctagccgt tccgcagacg ggatcgattt 6660
catgattttt tttgtttcgt tgcatagggt ttggtttgcc cttttccttt atttcaatat 6720
atgccgtgca cttgtttgtc gggtcatctt ttcatgcttt tttttgtctt ggttgtgatg 6780
atgtggtctg gttgggcggt cgttctagat cggagtagaa ttctgtttca aactacctgg 6840
tggatttatt aattttggat ctgtatgtgt gtgccataca tattcatagt tacgaattga 6900
agatgatgga tggaaatatc gatctaggat aggtatacat gttgatgcgg gttttactga 6960
tgcatataca gagatgcttt ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt 7020
cgttcattcg ttctagatcg gagtagaata ctgtttcaaa ctacctggtg tatttattaa 7080
ttttggaact gtatgtgtgt gtcatacatc ttcatagtta cgagtttaag atggatggaa 7140
atatcgatct aggataggta tacatgttga tgtgggtttt actgatgcat atacatgatg 7200
gcatatgcag catctattca tatgctctaa ccttgagtac ctatctatta taataaacaa 7260
gtatgtttta taattatttt gatcttgata tacttggatg atggcatatg cagcagctat 7320
atgtggattt ttttagccct gccttcatac gctatttatt tgcttggtac tgtttctttt 7380
gtcgatgctc accctgttgt ttggtgttac ttctgcaggg atctccgatc atgcaaaaac 7440
tcattaactc agtgcaaaac tatgcctggg gcagcaaaac ggcgttgact gaactttatg 7500
gtatggaaaa tccgtccagc cagccgatgg ccgagctgtg gatgggcgca catccgaaaa 7560
gcagttcacg agtgcagaat gccgccggag atatcgtttc actgcgtgat gtgattgaga 7620
gtgataaatc gactctgctc ggagaggccg ttgccaaacg ctttggcgaa ctgcctttcc 7680
tgttcaaagt attatgcgca gcacagccac tctccattca ggttcatcca aacaaacaca 7740
attctgaaat cggttttgcc aaagaaaatg ccgcaggtat cccgatggat gccgccgagc 7800
gtaactataa agatcctaac cacaagccgg agctggtttt tgcgctgacg cctttccttg 7860
cgatgaacgc gtttcgtgaa ttttccgaga ttgtctccct actccagccg gtcgcaggtg 7920
cacatccggc gattgctcac tttttacaac agcctgatgc cgaacgttta agcgaactgt 7980
tcgccagcct gttgaatatg cagggtgaag aaaaatcccg cgcgctggcg attttaaaat 8040
cggccctcga tagccagcag ggtgaaccgt ggcaaacgat tcgtttaatt tctgaatttt 8100
acccggaaga cagcggtctg ttctccccgc tattgctgaa tgtggtgaaa ttgaaccctg 8160
gcgaagcgat gttcctgttc gctgaaacac cgcacgctta cctgcaaggc gtggcgctgg 8220
aagtgatggc aaactccgat aacgtgctgc gtgcgggtct gacgcctaaa tacattgata 8280
ttccggaact ggttgccaat gtgaaattcg aagccaaacc ggctaaccag ttgttgaccc 8340
agccggtgaa acaaggtgca gaactggact tcccgattcc agtggatgat tttgccttct 8400
cgctgcatga ccttagtgat aaagaaacca ccattagcca gcagagtgcc gccattttgt 8460
tctgcgtcga aggcgatgca acgttgtgga aaggttctca gcagttacag cttaaaccgg 8520
gtgaatcagc gtttattgcc gccaacgaat caccggtgac tgtcaaaggc cacggccgtt 8580
tagcgcgtgt ttacaacaag ctgtaagagc ttactgaaaa aattaacatc tcttgctaag 8640
ctgggtcatg ggtcgtttaa gctgccgatg tgcctgcgtc gtctggtgcc ctctctccat 8700
atggaggttg tcaaagtatc tgctgttcgt gtcatgagtc gtgtcagtgt tggtttaata 8760
atggaccggt tgtgttgtgt gtgcgtacta cccagaacta tgacaaatca tgaataagtt 8820
tgatgtttga aattaaagcc tgtgctcatt atgttctgtc tttcagttgt ctcctaatat 8880
ttgcctccag gtactggcta tctaccgttt cttacttagg aggtgtttga atgcactaaa 8940
actaatagtt agtggctaaa attagttaaa acatccaaac accatagcta atagttgaac 9000
tattagctat ttttggaaaa ttagttaata gtgaggtagt tatttgttag ctagctaatt 9060
caactaacaa tttttagcca actaacaatt agtttcagtg cattcaaaca cccccttaat 9120
gttaacgtgg ttctatctac cgtctcctaa tatatggttg attgttcggt ttgttgctat 9180
gctattgggt tctgattgct gctagttctt gctgaatcca gaagttctcg tagtatagct 9240
cagattcata ttatttattt gagtgataag tgatccaggt tattactatg ttagctaggt 9300
tttttttaca aggataaatt atctgtgatc ataattctta tgaaagcttt atgtttcctg 9360
gaggcagtgg catgcaatgc atgacagcaa cttgatcaca ccagctgagg tagatacggt 9420
aacaaggttc ttaaatctgt tcaccaaatc attggagaac acacatacac attcttgcca 9480
gtcttggtta gagaaatttc atgacaaaat gccaaagctg tcttgactct tcacttttgg 9540
ccatgagtcg tgacttagtt tggtttaatg gaccggttct cctagcttgt tctactcaaa 9600
actgttgttg atgcgaataa gttgtgatgg ttgatctctg gattttgttt tgctctcaat 9660
agtggacgag attagatagc ctgcaggccc gggggcgcgc cctaattagc taacggccag 9720
gatcgccgcg tgagccttta gcaactagct agattaatta acgcaatctg ttattaagtt 9780
gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc 9840
ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gaattaattc 9900
tcatgtttga cagcttatca tcgactgcac ggtgcaccaa tgcttctggc gtcaggcagc 9960
catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac tgcataattc gtgtcgctca 10020
aggcgcactc ccgttctgga taatgttttt tgcgccgaca tcataacggt tctggcaaat 10080
attctgaaat gagctgttga caattaatca tccggctcgt ataatgtgtg gaattgtgag 10140
cggataacaa tttcacacag gaaacagacc atgagggaag cgttgatcgc cgaagtatcg 10200
actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc 10260
gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg 10320
ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt 10380
ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt 10440
gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga 10500
gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat 10560
ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg 10620
gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta 10680
acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg 10740
tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac 10800
tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc taggcaggct 10860
tatcttggac aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac 10920
tacgtgaaag gcgagatcac caaagtagtc ggcaaataaa gctctagtgg atctccgtac 10980
ccagggatct ggctcgcggc ggacgcacga cgccggggcg agaccatagg cgatctccta 11040
aatcaatagt agctgtaacc tcgaagcgtt tcacttgtaa caacgattga gaatttttgt 11100
cataaaattg aaatacttgg ttcgcatttt tgtcatccgc ggtcagccgc aattctgacg 11160
aactgcccat ttagctggag atgattgtac atccttcacg tgaaaatttc tcaagcgctg 11220
tgaacaaggg ttcagatttt agattgaaag gtgagccgtt gaaacacgtt cttcttgtcg 11280
atgacgacgt cgctatgcgg catcttatta ttgaatacct tacgatccac gccttcaaag 11340
tgaccgcggt agccgacagc acccagttca caagagtact ctcttccgcg acggtcgatg 11400
tcgtggttgt tgatctagat ttaggtcgtg aagatgggct cgagatcgtt cgtaatctgg 11460
cggcaaagtc tgatattcca atcataatta tcagtggcga ccgccttgag gagacggata 11520
aagttgttgc actcgagcta ggagcaagtg attttatcgc taagccgttc agtatcagag 11580
agtttctagc acgcattcgg gttgccttgc gcgtgcgccc caacgttgtc cgctccaaag 11640
accgacggtc tttttgtttt actgactgga cacttaatct caggcaacgt cgcttgatgt 11700
ccgaagctgg cggtgaggtg aaacttacgg caggtgagtt caatcttctc ctcgcgtttt 11760
tagagaaacc ccgcgacgtt ctatcgcgcg agcaacttct cattgccagt cgagtacgcg 11820
acgaggaggt ttatgacagg agtatagatg ttctcatttt gaggctgcgc cgcaaacttg 11880
aggcagatcc gtcaagccct caactgataa aaacagcaag aggtgccggt tatttctttg 11940
acgcggacgt gcaggtttcg cacgggggga cgatggcagc ctgagccaat tcccagatcc 12000
ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct 12060
gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga 12120
ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc 12180
ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca 12240
accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat 12300
ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta 12360
cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg 12420
ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg 12480
ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa 12540
gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg 12600
gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt 12660
gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg 12720
gcggccggag tacatcgaga tcgagctggc tgattggatg taccgcgaga tcacagaagg 12780
caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg 12840
ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt 12900
caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt 12960
gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca 13020
ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg 13080
ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa 13140
aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg 13200
gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac 13260
tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac 13320
tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca 13380
aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat 13440
cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg 13500
acaagccgcg ccgtcgccac tcgaccgccg gcgctgaggt ctgcctcgtg aagaaggtgt 13560
tgctgactca taccaggcct gaatcgcccc atcatccagc cagaaagtga gggagccacg 13620
gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt gctttgccac 13680
ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag caaaagttcg 13740
atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac 13800
caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc 13860
atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac 13920
tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt 13980
ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa 14040
tcaccatgag tgacgactga atccggtgag aatggcaaaa gctctgcatt aatgaatcgg 14100
ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 14160
ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 14220
acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 14280
aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 14340
tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 14400
aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 14460
gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 14520
acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 14580
accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 14640
ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 14700
gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 14760
aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 14820
ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 14880
gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 14940
cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 15000
cttcacctag atccttttga tccggaatta 15030
<210> 56
<211> 15030
<212> DNA
<213> 人工序列
<220>
<223> 载体24383
<400> 56
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctagtcct ttctctctct ttctttctct ctgctttatt gaacttctca 1260
aagttactag gtacgccgct cgtcctcccc ccccccccct ctctaccttc tctagatcgg 1320
cgttccggtc catagttagg gcccggtagt tctacttctg ttcatgtttg tgttagatcc 1380
gtgtttgtgt tagatccgtg ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac 1440
acgttctgat tgctaacttg ccagtgtttc tctttgggga atcctgggat ggctctagcc 1500
gttccgcaga cgggatcgat ttcatgattt tttttgtttc gttgcatagg gtttggtttg 1560
cccttttcct ttatttcaat atatgccgtg cacttgtttg tcgggtcatc ttttcatgct 1620
tttttttgtc ttggttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag 1680
aattctgttt caaactacct ggtggattta ttaattttgg atctgtatgt gtgtgccata 1740
catattcata gttacgaatt gaagatgatg gatggaaata tcgatctagg ataggtatac 1800
atgttgatgc gggttttact gatgcatata cagagatgct ttttgttcgc ttggttgtga 1860
tgatgtggtg tggttgggcg gtcgttcatt cgttctagat cggagtagaa tactgtttca 1920
aactacctgg tgtatttatt aattttggaa ctgtatgtgt gtgtcataca tcttcatagt 1980
tacgagttta agatggatgg aaatatcgat ctaggatagg tatacatgtt gatgtgggtt 2040
ttactgatgc atatacatga tggcatatgc agcatctatt catatgctct aaccttgagt 2100
acctatctat tataataaac aagtatgttt tataattatt ttgatcttga tatacttgga 2160
tgatggcata tccagcagct atatgtggat ttttttagcc ctgccttcat acgctattta 2220
tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag 2280
gatcctctaa gtagcagcca tggacaacaa ccccaacatc aacgagtgca tcccctacaa 2340
ctgcctgagc aaccccgagg tggaggtgct gggcggcgag cgcatcgaga ccggctacac 2400
ccccatcgac atcagcctga gcctgaccca gttcctgctg agcgagttcg tgcccggcgc 2460
cggcttcgtg ctgggcctgg tggacatcat ctggggcatc ttcggcccca gccagtggga 2520
cgccttcctg gtgcagatcg agcagttgat aaaccaacgc atagaggaat tcgcccgcaa 2580
ccaggccatc agccgcctgg agggcctgag caacctgtac caaatctacg ccgagagctt 2640
ccgcgagtgg gaggccgacc ccaccaaccc cgccctgcgc gaggagatgc gcatccagtt 2700
caacgacatg aacagcgccc tgaccaccgc catccccctg ttcgccgtgc agaactacca 2760
ggtgcccctg ctgagcgtgt acgtgcaggc cgccaacctg cacctgagcg tgctgcgcga 2820
cgtcagcgtg ttcggccagc gctggggctt cgacgccgcc accatcaaca gccgctacaa 2880
cgacctgacc cgcctgatcg gcaactacac cgaccacgcc gtgcgctggt acaacaccgg 2940
cctggagcgc gtgtgggggc ccgacagccg cgactggatc aggtacaacc agttccgccg 3000
cgagctgacc ctgaccgtgc tggacatcgt gagcctgttc cccaactacg acagccgcac 3060
ctaccccatc cgcaccgtga gccagctgac ccgcgagatt tacaccaacc ccgtgctgga 3120
gaacttcgac ggcagcttcc gcggcagcgc ccagggcatc gagggcagca tccgcagccc 3180
ccacctgatg gacatcctga acagcatcac catctacacc gacgcccacc gcggcgagta 3240
ctactggagc ggccaccaga tcatggccag ccccgtcggc ttcagcggcc ccgagttcac 3300
cttccccctg tacggcacga tgggcaacgc tgcacctcag cagcgcatcg tggcacagct 3360
gggccaggga gtgtaccgca ccctgagcag caccctgtac cgtcgacctt tcaacatcgg 3420
catcaacaac cagcagctga gcgtgctgga cggcaccgag ttcgcctacg gcaccagcag 3480
caacctgccc agcgccgtgt accgcaagag cggcaccgtg gacagcctgg acgagatccc 3540
ccctcagaac aacaacgtgc cacctcgaca gggcttcagc caccgtctga gccacgtgag 3600
catgttccgc agtggcttca gcaacagcag cgtgagcatc atccgtgcac ctatgttcag 3660
ctggattcac cgcagtgccg agttcaacaa catcatcccc agcagccaga tcacccagat 3720
ccccctgacc aagagcacca acctgggcag cggcaccagc gtggtgaagg gccccggctt 3780
caccggcggc gacatcctgc gccgcaccag ccccggccag atcagcaccc tgcgcgtgaa 3840
catcaccgcc cccctgagcc agcgctaccg cgtccgcatc cgctacgcca gcaccaccaa 3900
cctgcagttc cacaccagca tcgacggccg ccccatcaac cagggcaact tcagcgccac 3960
catgagcagc ggcagcaacc tgcagagcgg cagcttccgc accgtgggct tcaccacccc 4020
cttcaacttc agcaacggca gcagcgtgtt caccctgagc gcccacgtgt tcaacagcgg 4080
caacgaggtg tacatcgacc gcatcgagtt cgtgcccgcc gaggtgacct tcgaggccga 4140
gtacgacctg gagagggctc agaaggccgt gaacgagctg ttcaccagca gcaaccagat 4200
cggcctgaag accgacgtga ccgactacca catcgaccag gtgagcaacc tggtggagtg 4260
cttaagcgac gagttctgcc tggacgagaa gaaggagctg agcgagaagg tgaagcacgc 4320
caagcgcctg agcgacgagc gcaacctgct gcaggacccc aacttccgcg gcatcaaccg 4380
ccagctggac cgcggctggc gaggcagcac cgatatcacc atccagggcg gcgacgacgt 4440
gttcaaggag aactacgtga ccctgctggg caccttcgac gagtgctacc ccacctacct 4500
gtaccagaag atcgacgaga gcaagctgaa ggcctacacc cgctaccagc tgcgcggcta 4560
catcgaggac agccaggacc tggaaatcta cctgatccgc tacaacgcga agcacgagac 4620
cgtgaacgtg cccggcaccg gcagcctgtg gcccctgagc gcccccagcc ccatcggcaa 4680
gtgcactagt caccacagcc accacttcag cctggacatc gacgtgggct gcaccgacct 4740
gaacgaggac ctgggcgtgt gggtgatctt caagatcaag acccaggacg gccacgcccg 4800
cctgggcaat ctagagttcc tggaggagaa gcccctggtg ggcgaggccc tggcccgcgt 4860
gaagcgtgct gagaagaagt ggcgcgacaa gcgcgagaag ctggagtggg agaccaacat 4920
cgtgtacaag gaggccaagg agagcgtgga cgccctgttc gtgaacagcc agtacgaccg 4980
cctgcaggcc gacaccaaca tcgccatgat ccacgccgcc gacaagcgcg tgcacagcat 5040
tcgcgaggcc tacctgcccg agctgagcgt gatccccggt gtgaacgccg ccatcttcga 5100
ggaactcgag ggccgcatct aggagctcta tgatcgttca aacatttggc aataaagttt 5160
cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta 5220
cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat 5280
gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa 5340
ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcaataga ctacggaccg 5400
cgatcgctta attaagcttg catgcctgca gtgcagcgtg acccggtcgt gcccctctct 5460
agagataatg agcattgcat gtctaagtta taaaaaatta ccacatattt tttttgtcac 5520
acttgtttga agtgcagttt atctatcttt atacatatat ttaaacttta ctctacgaat 5580
aatataatct atagtactac aataatatca gtgttttaga gaatcatata aatgaacagt 5640
tagacatggt ctaaaggaca attgagtatt ttgacaacag gactctacag ttttatcttt 5700
ttagtgtgca tgtgttctcc tttttttttg caaatagctt cacctatata atacttcatc 5760
cattttatta gtacatccat ttagggttta gggttaatgg tttttataga ctaatttttt 5820
tagtacatct attttattct attttagcct ctaaattaag aaaactaaaa ctctatttta 5880
gtttttttat ttaataattt agatataaaa tagaataaaa taaagtgact aaaaattaaa 5940
caaataccct ttaagaaatt aaaaaaacta aggaaacatt tttcttgttt cgagtagata 6000
atgccagcct gttaaacgcc gccgacgagt ctaacggaca ccaaccagcg aaccagcagc 6060
gtcgcgtcgg gccaagcgaa gcagacggca cggcatctct gtcgctgcct ctggacccct 6120
ctcgagagtt ccgctccacc gttggacttg ctccgctgtc ggcatccaga aattgcgtgg 6180
cggagcggca gacgtgagcc ggcacggcag gcggcctcct cctcctctca cggcaccggc 6240
agctacgggg gattcctttc ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata 6300
aatagacacc ccctccacac cctctttccc caacctcgtg ttgttcggag cgcacacaca 6360
cacaaccaga tctcccccaa atccacccgt cggcacctcc gcttcaaggt acgccgctcg 6420
tcctcccccc ccccccctct ctaccttctc tagatcggcg ttccggtcca tagttagggc 6480
ccggtagttc tacttctgtt catgtttgtg ttagatccgt gtttgtgtta gatccgtgct 6540
gttagcgttc gtacacggat gcgacctgta cgtcagacac gttctgattg ctaacttgcc 6600
agtgtttctc tttggggaat cctgggatgg ctctagccgt tccgcagacg ggatcgattt 6660
catgattttt tttgtttcgt tgcatagggt ttggtttgcc cttttccttt atttcaatat 6720
atgccgtgca cttgtttgtc gggtcatctt ttcatgcttt tttttgtctt ggttgtgatg 6780
atgtggtctg gttgggcggt cgttctagat cggagtagaa ttctgtttca aactacctgg 6840
tggatttatt aattttggat ctgtatgtgt gtgccataca tattcatagt tacgaattga 6900
agatgatgga tggaaatatc gatctaggat aggtatacat gttgatgcgg gttttactga 6960
tgcatataca gagatgcttt ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt 7020
cgttcattcg ttctagatcg gagtagaata ctgtttcaaa ctacctggtg tatttattaa 7080
ttttggaact gtatgtgtgt gtcatacatc ttcatagtta cgagtttaag atggatggaa 7140
atatcgatct aggataggta tacatgttga tgtgggtttt actgatgcat atacatgatg 7200
gcatatgcag catctattca tatgctctaa ccttgagtac ctatctatta taataaacaa 7260
gtatgtttta taattatttt gatcttgata tacttggatg atggcatatg cagcagctat 7320
atgtggattt ttttagccct gccttcatac gctatttatt tgcttggtac tgtttctttt 7380
gtcgatgctc accctgttgt ttggtgttac ttctgcaggg atctccgatc atgcaaaaac 7440
tcattaactc agtgcaaaac tatgcctggg gcagcaaaac ggcgttgact gaactttatg 7500
gtatggaaaa tccgtccagc cagccgatgg ccgagctgtg gatgggcgca catccgaaaa 7560
gcagttcacg agtgcagaat gccgccggag atatcgtttc actgcgtgat gtgattgaga 7620
gtgataaatc gactctgctc ggagaggccg ttgccaaacg ctttggcgaa ctgcctttcc 7680
tgttcaaagt attatgcgca gcacagccac tctccattca ggttcatcca aacaaacaca 7740
attctgaaat cggttttgcc aaagaaaatg ccgcaggtat cccgatggat gccgccgagc 7800
gtaactataa agatcctaac cacaagccgg agctggtttt tgcgctgacg cctttccttg 7860
cgatgaacgc gtttcgtgaa ttttccgaga ttgtctccct actccagccg gtcgcaggtg 7920
cacatccggc gattgctcac tttttacaac agcctgatgc cgaacgttta agcgaactgt 7980
tcgccagcct gttgaatatg cagggtgaag aaaaatcccg cgcgctggcg attttaaaat 8040
cggccctcga tagccagcag ggtgaaccgt ggcaaacgat tcgtttaatt tctgaatttt 8100
acccggaaga cagcggtctg ttctccccgc tattgctgaa tgtggtgaaa ttgaaccctg 8160
gcgaagcgat gttcctgttc gctgaaacac cgcacgctta cctgcaaggc gtggcgctgg 8220
aagtgatggc aaactccgat aacgtgctgc gtgcgggtct gacgcctaaa tacattgata 8280
ttccggaact ggttgccaat gtgaaattcg aagccaaacc ggctaaccag ttgttgaccc 8340
agccggtgaa acaaggtgca gaactggact tcccgattcc agtggatgat tttgccttct 8400
cgctgcatga ccttagtgat aaagaaacca ccattagcca gcagagtgcc gccattttgt 8460
tctgcgtcga aggcgatgca acgttgtgga aaggttctca gcagttacag cttaaaccgg 8520
gtgaatcagc gtttattgcc gccaacgaat caccggtgac tgtcaaaggc cacggccgtt 8580
tagcgcgtgt ttacaacaag ctgtaagagc ttactgaaaa aattaacatc tcttgctaag 8640
ctgggtcatg ggtcgtttaa gctgccgatg tgcctgcgtc gtctggtgcc ctctctccat 8700
atggaggttg tcaaagtatc tgctgttcgt gtcatgagtc gtgtcagtgt tggtttaata 8760
atggaccggt tgtgttgtgt gtgcgtacta cccagaacta tgacaaatca tgaataagtt 8820
tgatgtttga aattaaagcc tgtgctcatt atgttctgtc tttcagttgt ctcctaatat 8880
ttgcctccag gtactggcta tctaccgttt cttacttagg aggtgtttga atgcactaaa 8940
actaatagtt agtggctaaa attagttaaa acatccaaac accatagcta atagttgaac 9000
tattagctat ttttggaaaa ttagttaata gtgaggtagt tatttgttag ctagctaatt 9060
caactaacaa tttttagcca actaacaatt agtttcagtg cattcaaaca cccccttaat 9120
gttaacgtgg ttctatctac cgtctcctaa tatatggttg attgttcggt ttgttgctat 9180
gctattgggt tctgattgct gctagttctt gctgaatcca gaagttctcg tagtatagct 9240
cagattcata ttatttattt gagtgataag tgatccaggt tattactatg ttagctaggt 9300
tttttttaca aggataaatt atctgtgatc ataattctta tgaaagcttt atgtttcctg 9360
gaggcagtgg catgcaatgc atgacagcaa cttgatcaca ccagctgagg tagatacggt 9420
aacaaggttc ttaaatctgt tcaccaaatc attggagaac acacatacac attcttgcca 9480
gtcttggtta gagaaatttc atgacaaaat gccaaagctg tcttgactct tcacttttgg 9540
ccatgagtcg tgacttagtt tggtttaatg gaccggttct cctagcttgt tctactcaaa 9600
actgttgttg atgcgaataa gttgtgatgg ttgatctctg gattttgttt tgctctcaat 9660
agtggacgag attagatagc ctgcaggccc gggggcgcgc cctaattagc taacggccag 9720
gatcgccgcg tgagccttta gcaactagct agattaatta acgcaatctg ttattaagtt 9780
gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc 9840
ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gaattaattc 9900
tcatgtttga cagcttatca tcgactgcac ggtgcaccaa tgcttctggc gtcaggcagc 9960
catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac tgcataattc gtgtcgctca 10020
aggcgcactc ccgttctgga taatgttttt tgcgccgaca tcataacggt tctggcaaat 10080
attctgaaat gagctgttga caattaatca tccggctcgt ataatgtgtg gaattgtgag 10140
cggataacaa tttcacacag gaaacagacc atgagggaag cgttgatcgc cgaagtatcg 10200
actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc 10260
gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg 10320
ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt 10380
ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt 10440
gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga 10500
gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat 10560
ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg 10620
gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta 10680
acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg 10740
tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac 10800
tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc taggcaggct 10860
tatcttggac aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac 10920
tacgtgaaag gcgagatcac caaagtagtc ggcaaataaa gctctagtgg atctccgtac 10980
ccagggatct ggctcgcggc ggacgcacga cgccggggcg agaccatagg cgatctccta 11040
aatcaatagt agctgtaacc tcgaagcgtt tcacttgtaa caacgattga gaatttttgt 11100
cataaaattg aaatacttgg ttcgcatttt tgtcatccgc ggtcagccgc aattctgacg 11160
aactgcccat ttagctggag atgattgtac atccttcacg tgaaaatttc tcaagcgctg 11220
tgaacaaggg ttcagatttt agattgaaag gtgagccgtt gaaacacgtt cttcttgtcg 11280
atgacgacgt cgctatgcgg catcttatta ttgaatacct tacgatccac gccttcaaag 11340
tgaccgcggt agccgacagc acccagttca caagagtact ctcttccgcg acggtcgatg 11400
tcgtggttgt tgatctagat ttaggtcgtg aagatgggct cgagatcgtt cgtaatctgg 11460
cggcaaagtc tgatattcca atcataatta tcagtggcga ccgccttgag gagacggata 11520
aagttgttgc actcgagcta ggagcaagtg attttatcgc taagccgttc agtatcagag 11580
agtttctagc acgcattcgg gttgccttgc gcgtgcgccc caacgttgtc cgctccaaag 11640
accgacggtc tttttgtttt actgactgga cacttaatct caggcaacgt cgcttgatgt 11700
ccgaagctgg cggtgaggtg aaacttacgg caggtgagtt caatcttctc ctcgcgtttt 11760
tagagaaacc ccgcgacgtt ctatcgcgcg agcaacttct cattgccagt cgagtacgcg 11820
acgaggaggt ttatgacagg agtatagatg ttctcatttt gaggctgcgc cgcaaacttg 11880
aggcagatcc gtcaagccct caactgataa aaacagcaag aggtgccggt tatttctttg 11940
acgcggacgt gcaggtttcg cacgggggga cgatggcagc ctgagccaat tcccagatcc 12000
ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct 12060
gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga 12120
ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc 12180
ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca 12240
accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat 12300
ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta 12360
cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg 12420
ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg 12480
ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa 12540
gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg 12600
gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt 12660
gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg 12720
gcggccggag tacatcgaga tcgagctggc tgattggatg taccgcgaga tcacagaagg 12780
caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg 12840
ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt 12900
caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt 12960
gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca 13020
ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg 13080
ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa 13140
aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg 13200
gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac 13260
tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac 13320
tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca 13380
aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat 13440
cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg 13500
acaagccgcg ccgtcgccac tcgaccgccg gcgctgaggt ctgcctcgtg aagaaggtgt 13560
tgctgactca taccaggcct gaatcgcccc atcatccagc cagaaagtga gggagccacg 13620
gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt gctttgccac 13680
ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag caaaagttcg 13740
atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac 13800
caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc 13860
atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac 13920
tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt 13980
ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa 14040
tcaccatgag tgacgactga atccggtgag aatggcaaaa gctctgcatt aatgaatcgg 14100
ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 14160
ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 14220
acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 14280
aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 14340
tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 14400
aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 14460
gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 14520
acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 14580
accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 14640
ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 14700
gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 14760
aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 14820
ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 14880
gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 14940
cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 15000
cttcacctag atccttttga tccggaatta 15030
<210> 57
<211> 15030
<212> DNA
<213> 人工序列
<220>
<223> 24372载体
<400> 57
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta 1260
gatcggcgtt ccggtccata gttagggccc ggtagttcta cttctgttca tgtttgtgtt 1320
agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg 1380
tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct 1440
ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt 1500
ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt 1560
catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg 1620
gagtagaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt 1680
gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag 1740
gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg 1800
ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact 1860
gtttcaaact acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt 1920
catagttacg agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg 1980
tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc 2040
ttgagtacct atctattata ataaacaagt atgttttata attattttga tcttgatata 2100
cttggatgat ggcatatcca gcagctatat gtggattttt ttagccctgc cttcatacgc 2160
tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt 2220
ctgcaggatc ctctaagtag cagccatgga caacaacccc aacatcaacg agtgcatccc 2280
ctacaactgc ctgagcaacc ccgaggtgga ggtgctgggc ggcgagcgca tcgagaccgg 2340
ctacaccccc atcgacatca gcctgagcct gacccagttc ctgctgagcg agttcgtgcc 2400
cggcgccggc ttcgtgctgg gcctggtgga catcatctgg ggcatcttcg gccccagcca 2460
gtgggacgcc ttcctggtgc agatcgagca gttgataaac caacgcatag aggaattcgc 2520
ccgcaaccag gccatcagcc gcctggaggg cctgagcaac ctgtaccaaa tctacgccga 2580
gagcttccgc gagtgggagg ccgaccccac caaccccgcc ctgcgcgagg agatgcgcat 2640
ccagttcaac gacatgaaca gcgccctgac caccgccatc cccctgttcg ccgtgcagaa 2700
ctaccaggtg cccctgctga gcgtgtacgt gcaggccgcc aacctgcacc tgagcgtgct 2760
gcgcgacgtc agcgtgttcg gccagcgctg gggcttcgac gccgccacca tcaacagccg 2820
ctacaacgac ctgacccgcc tgatcggcaa ctacaccgac cacgccgtgc gctggtacaa 2880
caccggcctg gagcgcgtgt gggggcccga cagccgcgac tggatcaggt acaaccagtt 2940
ccgccgcgag ctgaccctga ccgtgctgga catcgtgagc ctgttcccca actacgacag 3000
ccgcacctac cccatccgca ccgtgagcca gctgacccgc gagatttaca ccaaccccgt 3060
gctggagaac ttcgacggca gcttccgcgg cagcgcccag ggcatcgagg gcagcatccg 3120
cagcccccac ctgatggaca tcctgaacag catcaccatc tacaccgacg cccaccgcgg 3180
cgagtactac tggagcggcc accagatcat ggccagcccc gtcggcttca gcggccccga 3240
gttcaccttc cccctgtacg gcacgatggg caacgctgca cctcagcagc gcatcgtggc 3300
acagctgggc cagggagtgt accgcaccct gagcagcacc ctgtaccgtc gacctttcaa 3360
catcggcatc aacaaccagc agctgagcgt gctggacggc accgagttcg cctacggcac 3420
cagcagcaac ctgcccagcg ccgtgtaccg caagagcggc accgtggaca gcctggacga 3480
gatcccccct cagaacaaca acgtgccacc tcgacagggc ttcagccacc gtctgagcca 3540
cgtgagcatg ttccgcagtg gcttcagcaa cagcagcgtg agcatcatcc gtgcacctat 3600
gttcagctgg attcaccgca gtgccgagtt caacaacatc atccccagca gccagatcac 3660
ccagatcccc ctgaccaaga gcaccaacct gggcagcggc accagcgtgg tgaagggccc 3720
cggcttcacc ggcggcgaca tcctgcgccg caccagcccc ggccagatca gcaccctgcg 3780
cgtgaacatc accgcccccc tgagccagcg ctaccgcgtc cgcatccgct acgccagcac 3840
caccaacctg cagttccaca ccagcatcga cggccgcccc atcaaccagg gcaacttcag 3900
cgccaccatg agcagcggca gcaacctgca gagcggcagc ttccgcaccg tgggcttcac 3960
cacccccttc aacttcagca acggcagcag cgtgttcacc ctgagcgccc acgtgttcaa 4020
cagcggcaac gaggtgtaca tcgaccgcat cgagttcgtg cccgccgagg tgaccttcga 4080
ggccgagtac gacctggaga gggctcagaa ggccgtgaac gagctgttca ccagcagcaa 4140
ccagatcggc ctgaagaccg acgtgaccga ctaccacatc gaccaggtga gcaacctggt 4200
ggagtgctta agcgacgagt tctgcctgga cgagaagaag gagctgagcg agaaggtgaa 4260
gcacgccaag cgcctgagcg acgagcgcaa cctgctgcag gaccccaact tccgcggcat 4320
caaccgccag ctggaccgcg gctggcgagg cagcaccgat atcaccatcc agggcggcga 4380
cgacgtgttc aaggagaact acgtgaccct gctgggcacc ttcgacgagt gctaccccac 4440
ctacctgtac cagaagatcg acgagagcaa gctgaaggcc tacacccgct accagctgcg 4500
cggctacatc gaggacagcc aggacctgga aatctacctg atccgctaca acgcgaagca 4560
cgagaccgtg aacgtgcccg gcaccggcag cctgtggccc ctgagcgccc ccagccccat 4620
cggcaagtgc actagcgctc acgacagcct ggcagagctc ccttcactcc aaacattgcg 4680
catctctagt caccacagcc accacttcag cctggacatc gacgtgggct gcaccgacct 4740
gaacgaggac ctgggcgtgt gggtgatctt caagatcaag acccaggacg gccacgcccg 4800
cctgggcaat ctagagttcc tggaggagaa gcccctggtg ggcgaggccc tggcccgcgt 4860
gaagcgtgct gagaagaagt ggcgcgacaa gcgcgagaag ctggagtggg agaccaacat 4920
cgtgtacaag gaggccaagg agagcgtgga cgccctgttc gtgaacagcc agtacgaccg 4980
cctgcaggcc gacaccaaca tcgccatgat ccacgccgcc gacaagcgcg tgcacagcat 5040
tcgcgaggcc tacctgcccg agctgagcgt gatccccggt gtgaacgccg ccatcttcga 5100
ggaactcgag ggccgcatct aggagctcta tgatcgttca aacatttggc aataaagttt 5160
cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta 5220
cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat 5280
gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa 5340
ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcaataga ctacggaccg 5400
cgatcgctta attaagcttg catgcctgca gtgcagcgtg acccggtcgt gcccctctct 5460
agagataatg agcattgcat gtctaagtta taaaaaatta ccacatattt tttttgtcac 5520
acttgtttga agtgcagttt atctatcttt atacatatat ttaaacttta ctctacgaat 5580
aatataatct atagtactac aataatatca gtgttttaga gaatcatata aatgaacagt 5640
tagacatggt ctaaaggaca attgagtatt ttgacaacag gactctacag ttttatcttt 5700
ttagtgtgca tgtgttctcc tttttttttg caaatagctt cacctatata atacttcatc 5760
cattttatta gtacatccat ttagggttta gggttaatgg tttttataga ctaatttttt 5820
tagtacatct attttattct attttagcct ctaaattaag aaaactaaaa ctctatttta 5880
gtttttttat ttaataattt agatataaaa tagaataaaa taaagtgact aaaaattaaa 5940
caaataccct ttaagaaatt aaaaaaacta aggaaacatt tttcttgttt cgagtagata 6000
atgccagcct gttaaacgcc gccgacgagt ctaacggaca ccaaccagcg aaccagcagc 6060
gtcgcgtcgg gccaagcgaa gcagacggca cggcatctct gtcgctgcct ctggacccct 6120
ctcgagagtt ccgctccacc gttggacttg ctccgctgtc ggcatccaga aattgcgtgg 6180
cggagcggca gacgtgagcc ggcacggcag gcggcctcct cctcctctca cggcaccggc 6240
agctacgggg gattcctttc ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata 6300
aatagacacc ccctccacac cctctttccc caacctcgtg ttgttcggag cgcacacaca 6360
cacaaccaga tctcccccaa atccacccgt cggcacctcc gcttcaaggt acgccgctcg 6420
tcctcccccc ccccccctct ctaccttctc tagatcggcg ttccggtcca tagttagggc 6480
ccggtagttc tacttctgtt catgtttgtg ttagatccgt gtttgtgtta gatccgtgct 6540
gttagcgttc gtacacggat gcgacctgta cgtcagacac gttctgattg ctaacttgcc 6600
agtgtttctc tttggggaat cctgggatgg ctctagccgt tccgcagacg ggatcgattt 6660
catgattttt tttgtttcgt tgcatagggt ttggtttgcc cttttccttt atttcaatat 6720
atgccgtgca cttgtttgtc gggtcatctt ttcatgcttt tttttgtctt ggttgtgatg 6780
atgtggtctg gttgggcggt cgttctagat cggagtagaa ttctgtttca aactacctgg 6840
tggatttatt aattttggat ctgtatgtgt gtgccataca tattcatagt tacgaattga 6900
agatgatgga tggaaatatc gatctaggat aggtatacat gttgatgcgg gttttactga 6960
tgcatataca gagatgcttt ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt 7020
cgttcattcg ttctagatcg gagtagaata ctgtttcaaa ctacctggtg tatttattaa 7080
ttttggaact gtatgtgtgt gtcatacatc ttcatagtta cgagtttaag atggatggaa 7140
atatcgatct aggataggta tacatgttga tgtgggtttt actgatgcat atacatgatg 7200
gcatatgcag catctattca tatgctctaa ccttgagtac ctatctatta taataaacaa 7260
gtatgtttta taattatttt gatcttgata tacttggatg atggcatatg cagcagctat 7320
atgtggattt ttttagccct gccttcatac gctatttatt tgcttggtac tgtttctttt 7380
gtcgatgctc accctgttgt ttggtgttac ttctgcaggg atctccgatc atgcaaaaac 7440
tcattaactc agtgcaaaac tatgcctggg gcagcaaaac ggcgttgact gaactttatg 7500
gtatggaaaa tccgtccagc cagccgatgg ccgagctgtg gatgggcgca catccgaaaa 7560
gcagttcacg agtgcagaat gccgccggag atatcgtttc actgcgtgat gtgattgaga 7620
gtgataaatc gactctgctc ggagaggccg ttgccaaacg ctttggcgaa ctgcctttcc 7680
tgttcaaagt attatgcgca gcacagccac tctccattca ggttcatcca aacaaacaca 7740
attctgaaat cggttttgcc aaagaaaatg ccgcaggtat cccgatggat gccgccgagc 7800
gtaactataa agatcctaac cacaagccgg agctggtttt tgcgctgacg cctttccttg 7860
cgatgaacgc gtttcgtgaa ttttccgaga ttgtctccct actccagccg gtcgcaggtg 7920
cacatccggc gattgctcac tttttacaac agcctgatgc cgaacgttta agcgaactgt 7980
tcgccagcct gttgaatatg cagggtgaag aaaaatcccg cgcgctggcg attttaaaat 8040
cggccctcga tagccagcag ggtgaaccgt ggcaaacgat tcgtttaatt tctgaatttt 8100
acccggaaga cagcggtctg ttctccccgc tattgctgaa tgtggtgaaa ttgaaccctg 8160
gcgaagcgat gttcctgttc gctgaaacac cgcacgctta cctgcaaggc gtggcgctgg 8220
aagtgatggc aaactccgat aacgtgctgc gtgcgggtct gacgcctaaa tacattgata 8280
ttccggaact ggttgccaat gtgaaattcg aagccaaacc ggctaaccag ttgttgaccc 8340
agccggtgaa acaaggtgca gaactggact tcccgattcc agtggatgat tttgccttct 8400
cgctgcatga ccttagtgat aaagaaacca ccattagcca gcagagtgcc gccattttgt 8460
tctgcgtcga aggcgatgca acgttgtgga aaggttctca gcagttacag cttaaaccgg 8520
gtgaatcagc gtttattgcc gccaacgaat caccggtgac tgtcaaaggc cacggccgtt 8580
tagcgcgtgt ttacaacaag ctgtaagagc ttactgaaaa aattaacatc tcttgctaag 8640
ctgggtcatg ggtcgtttaa gctgccgatg tgcctgcgtc gtctggtgcc ctctctccat 8700
atggaggttg tcaaagtatc tgctgttcgt gtcatgagtc gtgtcagtgt tggtttaata 8760
atggaccggt tgtgttgtgt gtgcgtacta cccagaacta tgacaaatca tgaataagtt 8820
tgatgtttga aattaaagcc tgtgctcatt atgttctgtc tttcagttgt ctcctaatat 8880
ttgcctccag gtactggcta tctaccgttt cttacttagg aggtgtttga atgcactaaa 8940
actaatagtt agtggctaaa attagttaaa acatccaaac accatagcta atagttgaac 9000
tattagctat ttttggaaaa ttagttaata gtgaggtagt tatttgttag ctagctaatt 9060
caactaacaa tttttagcca actaacaatt agtttcagtg cattcaaaca cccccttaat 9120
gttaacgtgg ttctatctac cgtctcctaa tatatggttg attgttcggt ttgttgctat 9180
gctattgggt tctgattgct gctagttctt gctgaatcca gaagttctcg tagtatagct 9240
cagattcata ttatttattt gagtgataag tgatccaggt tattactatg ttagctaggt 9300
tttttttaca aggataaatt atctgtgatc ataattctta tgaaagcttt atgtttcctg 9360
gaggcagtgg catgcaatgc atgacagcaa cttgatcaca ccagctgagg tagatacggt 9420
aacaaggttc ttaaatctgt tcaccaaatc attggagaac acacatacac attcttgcca 9480
gtcttggtta gagaaatttc atgacaaaat gccaaagctg tcttgactct tcacttttgg 9540
ccatgagtcg tgacttagtt tggtttaatg gaccggttct cctagcttgt tctactcaaa 9600
actgttgttg atgcgaataa gttgtgatgg ttgatctctg gattttgttt tgctctcaat 9660
agtggacgag attagatagc ctgcaggccc gggggcgcgc cctaattagc taacggccag 9720
gatcgccgcg tgagccttta gcaactagct agattaatta acgcaatctg ttattaagtt 9780
gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc 9840
ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gaattaattc 9900
tcatgtttga cagcttatca tcgactgcac ggtgcaccaa tgcttctggc gtcaggcagc 9960
catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac tgcataattc gtgtcgctca 10020
aggcgcactc ccgttctgga taatgttttt tgcgccgaca tcataacggt tctggcaaat 10080
attctgaaat gagctgttga caattaatca tccggctcgt ataatgtgtg gaattgtgag 10140
cggataacaa tttcacacag gaaacagacc atgagggaag cgttgatcgc cgaagtatcg 10200
actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc 10260
gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg 10320
ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt 10380
ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt 10440
gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga 10500
gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat 10560
ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg 10620
gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta 10680
acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg 10740
tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac 10800
tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc taggcaggct 10860
tatcttggac aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac 10920
tacgtgaaag gcgagatcac caaagtagtc ggcaaataaa gctctagtgg atctccgtac 10980
ccagggatct ggctcgcggc ggacgcacga cgccggggcg agaccatagg cgatctccta 11040
aatcaatagt agctgtaacc tcgaagcgtt tcacttgtaa caacgattga gaatttttgt 11100
cataaaattg aaatacttgg ttcgcatttt tgtcatccgc ggtcagccgc aattctgacg 11160
aactgcccat ttagctggag atgattgtac atccttcacg tgaaaatttc tcaagcgctg 11220
tgaacaaggg ttcagatttt agattgaaag gtgagccgtt gaaacacgtt cttcttgtcg 11280
atgacgacgt cgctatgcgg catcttatta ttgaatacct tacgatccac gccttcaaag 11340
tgaccgcggt agccgacagc acccagttca caagagtact ctcttccgcg acggtcgatg 11400
tcgtggttgt tgatctagat ttaggtcgtg aagatgggct cgagatcgtt cgtaatctgg 11460
cggcaaagtc tgatattcca atcataatta tcagtggcga ccgccttgag gagacggata 11520
aagttgttgc actcgagcta ggagcaagtg attttatcgc taagccgttc agtatcagag 11580
agtttctagc acgcattcgg gttgccttgc gcgtgcgccc caacgttgtc cgctccaaag 11640
accgacggtc tttttgtttt actgactgga cacttaatct caggcaacgt cgcttgatgt 11700
ccgaagctgg cggtgaggtg aaacttacgg caggtgagtt caatcttctc ctcgcgtttt 11760
tagagaaacc ccgcgacgtt ctatcgcgcg agcaacttct cattgccagt cgagtacgcg 11820
acgaggaggt ttatgacagg agtatagatg ttctcatttt gaggctgcgc cgcaaacttg 11880
aggcagatcc gtcaagccct caactgataa aaacagcaag aggtgccggt tatttctttg 11940
acgcggacgt gcaggtttcg cacgggggga cgatggcagc ctgagccaat tcccagatcc 12000
ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct 12060
gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga 12120
ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc 12180
ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca 12240
accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat 12300
ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta 12360
cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg 12420
ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg 12480
ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa 12540
gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg 12600
gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt 12660
gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg 12720
gcggccggag tacatcgaga tcgagctggc tgattggatg taccgcgaga tcacagaagg 12780
caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg 12840
ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt 12900
caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt 12960
gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca 13020
ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg 13080
ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa 13140
aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg 13200
gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac 13260
tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac 13320
tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca 13380
aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat 13440
cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg 13500
acaagccgcg ccgtcgccac tcgaccgccg gcgctgaggt ctgcctcgtg aagaaggtgt 13560
tgctgactca taccaggcct gaatcgcccc atcatccagc cagaaagtga gggagccacg 13620
gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt gctttgccac 13680
ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag caaaagttcg 13740
atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac 13800
caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc 13860
atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac 13920
tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt 13980
ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa 14040
tcaccatgag tgacgactga atccggtgag aatggcaaaa gctctgcatt aatgaatcgg 14100
ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 14160
ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 14220
acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 14280
aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 14340
tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 14400
aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 14460
gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 14520
acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 14580
accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 14640
ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 14700
gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 14760
aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 14820
ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 14880
gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 14940
cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 15000
cttcacctag atccttttga tccggaatta 15030
<210> 58
<211> 15030
<212> DNA
<213> 人工序列
<220>
<223> 24378载体
<400> 58
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctagcgct cacgacagcc tggcagagct cccttcactc caaacattgc 1260
gcatctctag gtacgccgct cgtcctcccc ccccccccct ctctaccttc tctagatcgg 1320
cgttccggtc catagttagg gcccggtagt tctacttctg ttcatgtttg tgttagatcc 1380
gtgtttgtgt tagatccgtg ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac 1440
acgttctgat tgctaacttg ccagtgtttc tctttgggga atcctgggat ggctctagcc 1500
gttccgcaga cgggatcgat ttcatgattt tttttgtttc gttgcatagg gtttggtttg 1560
cccttttcct ttatttcaat atatgccgtg cacttgtttg tcgggtcatc ttttcatgct 1620
tttttttgtc ttggttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag 1680
aattctgttt caaactacct ggtggattta ttaattttgg atctgtatgt gtgtgccata 1740
catattcata gttacgaatt gaagatgatg gatggaaata tcgatctagg ataggtatac 1800
atgttgatgc gggttttact gatgcatata cagagatgct ttttgttcgc ttggttgtga 1860
tgatgtggtg tggttgggcg gtcgttcatt cgttctagat cggagtagaa tactgtttca 1920
aactacctgg tgtatttatt aattttggaa ctgtatgtgt gtgtcataca tcttcatagt 1980
tacgagttta agatggatgg aaatatcgat ctaggatagg tatacatgtt gatgtgggtt 2040
ttactgatgc atatacatga tggcatatgc agcatctatt catatgctct aaccttgagt 2100
acctatctat tataataaac aagtatgttt tataattatt ttgatcttga tatacttgga 2160
tgatggcata tccagcagct atatgtggat ttttttagcc ctgccttcat acgctattta 2220
tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag 2280
gatcctctaa gtagcagcca tggacaacaa ccccaacatc aacgagtgca tcccctacaa 2340
ctgcctgagc aaccccgagg tggaggtgct gggcggcgag cgcatcgaga ccggctacac 2400
ccccatcgac atcagcctga gcctgaccca gttcctgctg agcgagttcg tgcccggcgc 2460
cggcttcgtg ctgggcctgg tggacatcat ctggggcatc ttcggcccca gccagtggga 2520
cgccttcctg gtgcagatcg agcagttgat aaaccaacgc atagaggaat tcgcccgcaa 2580
ccaggccatc agccgcctgg agggcctgag caacctgtac caaatctacg ccgagagctt 2640
ccgcgagtgg gaggccgacc ccaccaaccc cgccctgcgc gaggagatgc gcatccagtt 2700
caacgacatg aacagcgccc tgaccaccgc catccccctg ttcgccgtgc agaactacca 2760
ggtgcccctg ctgagcgtgt acgtgcaggc cgccaacctg cacctgagcg tgctgcgcga 2820
cgtcagcgtg ttcggccagc gctggggctt cgacgccgcc accatcaaca gccgctacaa 2880
cgacctgacc cgcctgatcg gcaactacac cgaccacgcc gtgcgctggt acaacaccgg 2940
cctggagcgc gtgtgggggc ccgacagccg cgactggatc aggtacaacc agttccgccg 3000
cgagctgacc ctgaccgtgc tggacatcgt gagcctgttc cccaactacg acagccgcac 3060
ctaccccatc cgcaccgtga gccagctgac ccgcgagatt tacaccaacc ccgtgctgga 3120
gaacttcgac ggcagcttcc gcggcagcgc ccagggcatc gagggcagca tccgcagccc 3180
ccacctgatg gacatcctga acagcatcac catctacacc gacgcccacc gcggcgagta 3240
ctactggagc ggccaccaga tcatggccag ccccgtcggc ttcagcggcc ccgagttcac 3300
cttccccctg tacggcacga tgggcaacgc tgcacctcag cagcgcatcg tggcacagct 3360
gggccaggga gtgtaccgca ccctgagcag caccctgtac cgtcgacctt tcaacatcgg 3420
catcaacaac cagcagctga gcgtgctgga cggcaccgag ttcgcctacg gcaccagcag 3480
caacctgccc agcgccgtgt accgcaagag cggcaccgtg gacagcctgg acgagatccc 3540
ccctcagaac aacaacgtgc cacctcgaca gggcttcagc caccgtctga gccacgtgag 3600
catgttccgc agtggcttca gcaacagcag cgtgagcatc atccgtgcac ctatgttcag 3660
ctggattcac cgcagtgccg agttcaacaa catcatcccc agcagccaga tcacccagat 3720
ccccctgacc aagagcacca acctgggcag cggcaccagc gtggtgaagg gccccggctt 3780
caccggcggc gacatcctgc gccgcaccag ccccggccag atcagcaccc tgcgcgtgaa 3840
catcaccgcc cccctgagcc agcgctaccg cgtccgcatc cgctacgcca gcaccaccaa 3900
cctgcagttc cacaccagca tcgacggccg ccccatcaac cagggcaact tcagcgccac 3960
catgagcagc ggcagcaacc tgcagagcgg cagcttccgc accgtgggct tcaccacccc 4020
cttcaacttc agcaacggca gcagcgtgtt caccctgagc gcccacgtgt tcaacagcgg 4080
caacgaggtg tacatcgacc gcatcgagtt cgtgcccgcc gaggtgacct tcgaggccga 4140
gtacgacctg gagagggctc agaaggccgt gaacgagctg ttcaccagca gcaaccagat 4200
cggcctgaag accgacgtga ccgactacca catcgaccag gtgagcaacc tggtggagtg 4260
cttaagcgac gagttctgcc tggacgagaa gaaggagctg agcgagaagg tgaagcacgc 4320
caagcgcctg agcgacgagc gcaacctgct gcaggacccc aacttccgcg gcatcaaccg 4380
ccagctggac cgcggctggc gaggcagcac cgatatcacc atccagggcg gcgacgacgt 4440
gttcaaggag aactacgtga ccctgctggg caccttcgac gagtgctacc ccacctacct 4500
gtaccagaag atcgacgaga gcaagctgaa ggcctacacc cgctaccagc tgcgcggcta 4560
catcgaggac agccaggacc tggaaatcta cctgatccgc tacaacgcga agcacgagac 4620
cgtgaacgtg cccggcaccg gcagcctgtg gcccctgagc gcccccagcc ccatcggcaa 4680
gtgcactagt caccacagcc accacttcag cctggacatc gacgtgggct gcaccgacct 4740
gaacgaggac ctgggcgtgt gggtgatctt caagatcaag acccaggacg gccacgcccg 4800
cctgggcaat ctagagttcc tggaggagaa gcccctggtg ggcgaggccc tggcccgcgt 4860
gaagcgtgct gagaagaagt ggcgcgacaa gcgcgagaag ctggagtggg agaccaacat 4920
cgtgtacaag gaggccaagg agagcgtgga cgccctgttc gtgaacagcc agtacgaccg 4980
cctgcaggcc gacaccaaca tcgccatgat ccacgccgcc gacaagcgcg tgcacagcat 5040
tcgcgaggcc tacctgcccg agctgagcgt gatccccggt gtgaacgccg ccatcttcga 5100
ggaactcgag ggccgcatct aggagctcta tgatcgttca aacatttggc aataaagttt 5160
cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta 5220
cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat 5280
gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa 5340
ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcaataga ctacggaccg 5400
cgatcgctta attaagcttg catgcctgca gtgcagcgtg acccggtcgt gcccctctct 5460
agagataatg agcattgcat gtctaagtta taaaaaatta ccacatattt tttttgtcac 5520
acttgtttga agtgcagttt atctatcttt atacatatat ttaaacttta ctctacgaat 5580
aatataatct atagtactac aataatatca gtgttttaga gaatcatata aatgaacagt 5640
tagacatggt ctaaaggaca attgagtatt ttgacaacag gactctacag ttttatcttt 5700
ttagtgtgca tgtgttctcc tttttttttg caaatagctt cacctatata atacttcatc 5760
cattttatta gtacatccat ttagggttta gggttaatgg tttttataga ctaatttttt 5820
tagtacatct attttattct attttagcct ctaaattaag aaaactaaaa ctctatttta 5880
gtttttttat ttaataattt agatataaaa tagaataaaa taaagtgact aaaaattaaa 5940
caaataccct ttaagaaatt aaaaaaacta aggaaacatt tttcttgttt cgagtagata 6000
atgccagcct gttaaacgcc gccgacgagt ctaacggaca ccaaccagcg aaccagcagc 6060
gtcgcgtcgg gccaagcgaa gcagacggca cggcatctct gtcgctgcct ctggacccct 6120
ctcgagagtt ccgctccacc gttggacttg ctccgctgtc ggcatccaga aattgcgtgg 6180
cggagcggca gacgtgagcc ggcacggcag gcggcctcct cctcctctca cggcaccggc 6240
agctacgggg gattcctttc ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata 6300
aatagacacc ccctccacac cctctttccc caacctcgtg ttgttcggag cgcacacaca 6360
cacaaccaga tctcccccaa atccacccgt cggcacctcc gcttcaaggt acgccgctcg 6420
tcctcccccc ccccccctct ctaccttctc tagatcggcg ttccggtcca tagttagggc 6480
ccggtagttc tacttctgtt catgtttgtg ttagatccgt gtttgtgtta gatccgtgct 6540
gttagcgttc gtacacggat gcgacctgta cgtcagacac gttctgattg ctaacttgcc 6600
agtgtttctc tttggggaat cctgggatgg ctctagccgt tccgcagacg ggatcgattt 6660
catgattttt tttgtttcgt tgcatagggt ttggtttgcc cttttccttt atttcaatat 6720
atgccgtgca cttgtttgtc gggtcatctt ttcatgcttt tttttgtctt ggttgtgatg 6780
atgtggtctg gttgggcggt cgttctagat cggagtagaa ttctgtttca aactacctgg 6840
tggatttatt aattttggat ctgtatgtgt gtgccataca tattcatagt tacgaattga 6900
agatgatgga tggaaatatc gatctaggat aggtatacat gttgatgcgg gttttactga 6960
tgcatataca gagatgcttt ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt 7020
cgttcattcg ttctagatcg gagtagaata ctgtttcaaa ctacctggtg tatttattaa 7080
ttttggaact gtatgtgtgt gtcatacatc ttcatagtta cgagtttaag atggatggaa 7140
atatcgatct aggataggta tacatgttga tgtgggtttt actgatgcat atacatgatg 7200
gcatatgcag catctattca tatgctctaa ccttgagtac ctatctatta taataaacaa 7260
gtatgtttta taattatttt gatcttgata tacttggatg atggcatatg cagcagctat 7320
atgtggattt ttttagccct gccttcatac gctatttatt tgcttggtac tgtttctttt 7380
gtcgatgctc accctgttgt ttggtgttac ttctgcaggg atctccgatc atgcaaaaac 7440
tcattaactc agtgcaaaac tatgcctggg gcagcaaaac ggcgttgact gaactttatg 7500
gtatggaaaa tccgtccagc cagccgatgg ccgagctgtg gatgggcgca catccgaaaa 7560
gcagttcacg agtgcagaat gccgccggag atatcgtttc actgcgtgat gtgattgaga 7620
gtgataaatc gactctgctc ggagaggccg ttgccaaacg ctttggcgaa ctgcctttcc 7680
tgttcaaagt attatgcgca gcacagccac tctccattca ggttcatcca aacaaacaca 7740
attctgaaat cggttttgcc aaagaaaatg ccgcaggtat cccgatggat gccgccgagc 7800
gtaactataa agatcctaac cacaagccgg agctggtttt tgcgctgacg cctttccttg 7860
cgatgaacgc gtttcgtgaa ttttccgaga ttgtctccct actccagccg gtcgcaggtg 7920
cacatccggc gattgctcac tttttacaac agcctgatgc cgaacgttta agcgaactgt 7980
tcgccagcct gttgaatatg cagggtgaag aaaaatcccg cgcgctggcg attttaaaat 8040
cggccctcga tagccagcag ggtgaaccgt ggcaaacgat tcgtttaatt tctgaatttt 8100
acccggaaga cagcggtctg ttctccccgc tattgctgaa tgtggtgaaa ttgaaccctg 8160
gcgaagcgat gttcctgttc gctgaaacac cgcacgctta cctgcaaggc gtggcgctgg 8220
aagtgatggc aaactccgat aacgtgctgc gtgcgggtct gacgcctaaa tacattgata 8280
ttccggaact ggttgccaat gtgaaattcg aagccaaacc ggctaaccag ttgttgaccc 8340
agccggtgaa acaaggtgca gaactggact tcccgattcc agtggatgat tttgccttct 8400
cgctgcatga ccttagtgat aaagaaacca ccattagcca gcagagtgcc gccattttgt 8460
tctgcgtcga aggcgatgca acgttgtgga aaggttctca gcagttacag cttaaaccgg 8520
gtgaatcagc gtttattgcc gccaacgaat caccggtgac tgtcaaaggc cacggccgtt 8580
tagcgcgtgt ttacaacaag ctgtaagagc ttactgaaaa aattaacatc tcttgctaag 8640
ctgggtcatg ggtcgtttaa gctgccgatg tgcctgcgtc gtctggtgcc ctctctccat 8700
atggaggttg tcaaagtatc tgctgttcgt gtcatgagtc gtgtcagtgt tggtttaata 8760
atggaccggt tgtgttgtgt gtgcgtacta cccagaacta tgacaaatca tgaataagtt 8820
tgatgtttga aattaaagcc tgtgctcatt atgttctgtc tttcagttgt ctcctaatat 8880
ttgcctccag gtactggcta tctaccgttt cttacttagg aggtgtttga atgcactaaa 8940
actaatagtt agtggctaaa attagttaaa acatccaaac accatagcta atagttgaac 9000
tattagctat ttttggaaaa ttagttaata gtgaggtagt tatttgttag ctagctaatt 9060
caactaacaa tttttagcca actaacaatt agtttcagtg cattcaaaca cccccttaat 9120
gttaacgtgg ttctatctac cgtctcctaa tatatggttg attgttcggt ttgttgctat 9180
gctattgggt tctgattgct gctagttctt gctgaatcca gaagttctcg tagtatagct 9240
cagattcata ttatttattt gagtgataag tgatccaggt tattactatg ttagctaggt 9300
tttttttaca aggataaatt atctgtgatc ataattctta tgaaagcttt atgtttcctg 9360
gaggcagtgg catgcaatgc atgacagcaa cttgatcaca ccagctgagg tagatacggt 9420
aacaaggttc ttaaatctgt tcaccaaatc attggagaac acacatacac attcttgcca 9480
gtcttggtta gagaaatttc atgacaaaat gccaaagctg tcttgactct tcacttttgg 9540
ccatgagtcg tgacttagtt tggtttaatg gaccggttct cctagcttgt tctactcaaa 9600
actgttgttg atgcgaataa gttgtgatgg ttgatctctg gattttgttt tgctctcaat 9660
agtggacgag attagatagc ctgcaggccc gggggcgcgc cctaattagc taacggccag 9720
gatcgccgcg tgagccttta gcaactagct agattaatta acgcaatctg ttattaagtt 9780
gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc 9840
ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gaattaattc 9900
tcatgtttga cagcttatca tcgactgcac ggtgcaccaa tgcttctggc gtcaggcagc 9960
catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac tgcataattc gtgtcgctca 10020
aggcgcactc ccgttctgga taatgttttt tgcgccgaca tcataacggt tctggcaaat 10080
attctgaaat gagctgttga caattaatca tccggctcgt ataatgtgtg gaattgtgag 10140
cggataacaa tttcacacag gaaacagacc atgagggaag cgttgatcgc cgaagtatcg 10200
actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc 10260
gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg 10320
ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt 10380
ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt 10440
gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga 10500
gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat 10560
ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg 10620
gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta 10680
acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg 10740
tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac 10800
tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc taggcaggct 10860
tatcttggac aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac 10920
tacgtgaaag gcgagatcac caaagtagtc ggcaaataaa gctctagtgg atctccgtac 10980
ccagggatct ggctcgcggc ggacgcacga cgccggggcg agaccatagg cgatctccta 11040
aatcaatagt agctgtaacc tcgaagcgtt tcacttgtaa caacgattga gaatttttgt 11100
cataaaattg aaatacttgg ttcgcatttt tgtcatccgc ggtcagccgc aattctgacg 11160
aactgcccat ttagctggag atgattgtac atccttcacg tgaaaatttc tcaagcgctg 11220
tgaacaaggg ttcagatttt agattgaaag gtgagccgtt gaaacacgtt cttcttgtcg 11280
atgacgacgt cgctatgcgg catcttatta ttgaatacct tacgatccac gccttcaaag 11340
tgaccgcggt agccgacagc acccagttca caagagtact ctcttccgcg acggtcgatg 11400
tcgtggttgt tgatctagat ttaggtcgtg aagatgggct cgagatcgtt cgtaatctgg 11460
cggcaaagtc tgatattcca atcataatta tcagtggcga ccgccttgag gagacggata 11520
aagttgttgc actcgagcta ggagcaagtg attttatcgc taagccgttc agtatcagag 11580
agtttctagc acgcattcgg gttgccttgc gcgtgcgccc caacgttgtc cgctccaaag 11640
accgacggtc tttttgtttt actgactgga cacttaatct caggcaacgt cgcttgatgt 11700
ccgaagctgg cggtgaggtg aaacttacgg caggtgagtt caatcttctc ctcgcgtttt 11760
tagagaaacc ccgcgacgtt ctatcgcgcg agcaacttct cattgccagt cgagtacgcg 11820
acgaggaggt ttatgacagg agtatagatg ttctcatttt gaggctgcgc cgcaaacttg 11880
aggcagatcc gtcaagccct caactgataa aaacagcaag aggtgccggt tatttctttg 11940
acgcggacgt gcaggtttcg cacgggggga cgatggcagc ctgagccaat tcccagatcc 12000
ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct 12060
gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga 12120
ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc 12180
ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca 12240
accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat 12300
ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta 12360
cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg 12420
ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg 12480
ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa 12540
gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg 12600
gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt 12660
gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg 12720
gcggccggag tacatcgaga tcgagctggc tgattggatg taccgcgaga tcacagaagg 12780
caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg 12840
ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt 12900
caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt 12960
gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca 13020
ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg 13080
ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa 13140
aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg 13200
gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac 13260
tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac 13320
tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca 13380
aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat 13440
cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg 13500
acaagccgcg ccgtcgccac tcgaccgccg gcgctgaggt ctgcctcgtg aagaaggtgt 13560
tgctgactca taccaggcct gaatcgcccc atcatccagc cagaaagtga gggagccacg 13620
gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt gctttgccac 13680
ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag caaaagttcg 13740
atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac 13800
caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc 13860
atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac 13920
tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt 13980
ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa 14040
tcaccatgag tgacgactga atccggtgag aatggcaaaa gctctgcatt aatgaatcgg 14100
ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 14160
ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 14220
acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 14280
aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 14340
tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 14400
aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 14460
gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 14520
acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 14580
accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 14640
ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 14700
gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 14760
aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 14820
ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 14880
gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 14940
cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 15000
cttcacctag atccttttga tccggaatta 15030
<210> 59
<211> 15033
<212> DNA
<213> 人工序列
<220>
<223> 24382载体
<400> 59
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctagcgac gaccccaaag gacaggtttc ccacagcttt attgaacaat 1260
tgtttgcatc taggtacgcc gctcgtcctc cccccccccc cctctctacc ttctctagat 1320
cggcgttccg gtccatagtt agggcccggt agttctactt ctgttcatgt ttgtgttaga 1380
tccgtgtttg tgttagatcc gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca 1440
gacacgttct gattgctaac ttgccagtgt ttctctttgg ggaatcctgg gatggctcta 1500
gccgttccgc agacgggatc gatttcatga ttttttttgt ttcgttgcat agggtttggt 1560
ttgccctttt cctttatttc aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat 1620
gctttttttt gtcttggttg tgatgatgtg gtctggttgg gcggtcgttc tagatcggag 1680
tagaattctg tttcaaacta cctggtggat ttattaattt tggatctgta tgtgtgtgcc 1740
atacatattc atagttacga attgaagatg atggatggaa atatcgatct aggataggta 1800
tacatgttga tgcgggtttt actgatgcat atacagagat gctttttgtt cgcttggttg 1860
tgatgatgtg gtgtggttgg gcggtcgttc attcgttcta gatcggagta gaatactgtt 1920
tcaaactacc tggtgtattt attaattttg gaactgtatg tgtgtgtcat acatcttcat 1980
agttacgagt ttaagatgga tggaaatatc gatctaggat aggtatacat gttgatgtgg 2040
gttttactga tgcatataca tgatggcata tgcagcatct attcatatgc tctaaccttg 2100
agtacctatc tattataata aacaagtatg ttttataatt attttgatct tgatatactt 2160
ggatgatggc atatccagca gctatatgtg gattttttta gccctgcctt catacgctat 2220
ttatttgctt ggtactgttt cttttgtcga tgctcaccct gttgtttggt gttacttctg 2280
caggatcctc taagtagcag ccatggacaa caaccccaac atcaacgagt gcatccccta 2340
caactgcctg agcaaccccg aggtggaggt gctgggcggc gagcgcatcg agaccggcta 2400
cacccccatc gacatcagcc tgagcctgac ccagttcctg ctgagcgagt tcgtgcccgg 2460
cgccggcttc gtgctgggcc tggtggacat catctggggc atcttcggcc ccagccagtg 2520
ggacgccttc ctggtgcaga tcgagcagtt gataaaccaa cgcatagagg aattcgcccg 2580
caaccaggcc atcagccgcc tggagggcct gagcaacctg taccaaatct acgccgagag 2640
cttccgcgag tgggaggccg accccaccaa ccccgccctg cgcgaggaga tgcgcatcca 2700
gttcaacgac atgaacagcg ccctgaccac cgccatcccc ctgttcgccg tgcagaacta 2760
ccaggtgccc ctgctgagcg tgtacgtgca ggccgccaac ctgcacctga gcgtgctgcg 2820
cgacgtcagc gtgttcggcc agcgctgggg cttcgacgcc gccaccatca acagccgcta 2880
caacgacctg acccgcctga tcggcaacta caccgaccac gccgtgcgct ggtacaacac 2940
cggcctggag cgcgtgtggg ggcccgacag ccgcgactgg atcaggtaca accagttccg 3000
ccgcgagctg accctgaccg tgctggacat cgtgagcctg ttccccaact acgacagccg 3060
cacctacccc atccgcaccg tgagccagct gacccgcgag atttacacca accccgtgct 3120
ggagaacttc gacggcagct tccgcggcag cgcccagggc atcgagggca gcatccgcag 3180
cccccacctg atggacatcc tgaacagcat caccatctac accgacgccc accgcggcga 3240
gtactactgg agcggccacc agatcatggc cagccccgtc ggcttcagcg gccccgagtt 3300
caccttcccc ctgtacggca cgatgggcaa cgctgcacct cagcagcgca tcgtggcaca 3360
gctgggccag ggagtgtacc gcaccctgag cagcaccctg taccgtcgac ctttcaacat 3420
cggcatcaac aaccagcagc tgagcgtgct ggacggcacc gagttcgcct acggcaccag 3480
cagcaacctg cccagcgccg tgtaccgcaa gagcggcacc gtggacagcc tggacgagat 3540
cccccctcag aacaacaacg tgccacctcg acagggcttc agccaccgtc tgagccacgt 3600
gagcatgttc cgcagtggct tcagcaacag cagcgtgagc atcatccgtg cacctatgtt 3660
cagctggatt caccgcagtg ccgagttcaa caacatcatc cccagcagcc agatcaccca 3720
gatccccctg accaagagca ccaacctggg cagcggcacc agcgtggtga agggccccgg 3780
cttcaccggc ggcgacatcc tgcgccgcac cagccccggc cagatcagca ccctgcgcgt 3840
gaacatcacc gcccccctga gccagcgcta ccgcgtccgc atccgctacg ccagcaccac 3900
caacctgcag ttccacacca gcatcgacgg ccgccccatc aaccagggca acttcagcgc 3960
caccatgagc agcggcagca acctgcagag cggcagcttc cgcaccgtgg gcttcaccac 4020
ccccttcaac ttcagcaacg gcagcagcgt gttcaccctg agcgcccacg tgttcaacag 4080
cggcaacgag gtgtacatcg accgcatcga gttcgtgccc gccgaggtga ccttcgaggc 4140
cgagtacgac ctggagaggg ctcagaaggc cgtgaacgag ctgttcacca gcagcaacca 4200
gatcggcctg aagaccgacg tgaccgacta ccacatcgac caggtgagca acctggtgga 4260
gtgcttaagc gacgagttct gcctggacga gaagaaggag ctgagcgaga aggtgaagca 4320
cgccaagcgc ctgagcgacg agcgcaacct gctgcaggac cccaacttcc gcggcatcaa 4380
ccgccagctg gaccgcggct ggcgaggcag caccgatatc accatccagg gcggcgacga 4440
cgtgttcaag gagaactacg tgaccctgct gggcaccttc gacgagtgct accccaccta 4500
cctgtaccag aagatcgacg agagcaagct gaaggcctac acccgctacc agctgcgcgg 4560
ctacatcgag gacagccagg acctggaaat ctacctgatc cgctacaacg cgaagcacga 4620
gaccgtgaac gtgcccggca ccggcagcct gtggcccctg agcgccccca gccccatcgg 4680
caagtgcact agtcaccaca gccaccactt cagcctggac atcgacgtgg gctgcaccga 4740
cctgaacgag gacctgggcg tgtgggtgat cttcaagatc aagacccagg acggccacgc 4800
ccgcctgggc aatctagagt tcctggagga gaagcccctg gtgggcgagg ccctggcccg 4860
cgtgaagcgt gctgagaaga agtggcgcga caagcgcgag aagctggagt gggagaccaa 4920
catcgtgtac aaggaggcca aggagagcgt ggacgccctg ttcgtgaaca gccagtacga 4980
ccgcctgcag gccgacacca acatcgccat gatccacgcc gccgacaagc gcgtgcacag 5040
cattcgcgag gcctacctgc ccgagctgag cgtgatcccc ggtgtgaacg ccgccatctt 5100
cgaggaactc gagggccgca tctaggagct ctatgatcgt tcaaacattt ggcaataaag 5160
tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa 5220
ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt 5280
tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc 5340
aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcaat agactacgga 5400
ccgcgatcgc ttaattaagc ttgcatgcct gcagtgcagc gtgacccggt cgtgcccctc 5460
tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt 5520
cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg 5580
aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac 5640
agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc 5700
tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc 5760
atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt 5820
ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt 5880
ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt 5940
aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag 6000
ataatgccag cctgttaaac gccgccgacg agtctaacgg acaccaacca gcgaaccagc 6060
agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc 6120
cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg 6180
tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacc 6240
ggcagctacg ggggattcct ttcccaccgc tccttcgctt tcccttcctc gcccgccgta 6300
ataaatagac accccctcca caccctcttt ccccaacctc gtgttgttcg gagcgcacac 6360
acacacaacc agatctcccc caaatccacc cgtcggcacc tccgcttcaa ggtacgccgc 6420
tcgtcctccc cccccccccc tctctacctt ctctagatcg gcgttccggt ccatagttag 6480
ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt 6540
gctgttagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga ttgctaactt 6600
gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag acgggatcga 6660
tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc tttatttcaa 6720
tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt cttggttgtg 6780
atgatgtggt ctggttgggc ggtcgttcta gatcggagta gaattctgtt tcaaactacc 6840
tggtggattt attaattttg gatctgtatg tgtgtgccat acatattcat agttacgaat 6900
tgaagatgat ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac 6960
tgatgcatat acagagatgc tttttgttcg cttggttgtg atgatgtggt gtggttgggc 7020
ggtcgttcat tcgttctaga tcggagtaga atactgtttc aaactacctg gtgtatttat 7080
taattttgga actgtatgtg tgtgtcatac atcttcatag ttacgagttt aagatggatg 7140
gaaatatcga tctaggatag gtatacatgt tgatgtgggt tttactgatg catatacatg 7200
atggcatatg cagcatctat tcatatgctc taaccttgag tacctatcta ttataataaa 7260
caagtatgtt ttataattat tttgatcttg atatacttgg atgatggcat atgcagcagc 7320
tatatgtgga tttttttagc cctgccttca tacgctattt atttgcttgg tactgtttct 7380
tttgtcgatg ctcaccctgt tgtttggtgt tacttctgca gggatctccg atcatgcaaa 7440
aactcattaa ctcagtgcaa aactatgcct ggggcagcaa aacggcgttg actgaacttt 7500
atggtatgga aaatccgtcc agccagccga tggccgagct gtggatgggc gcacatccga 7560
aaagcagttc acgagtgcag aatgccgccg gagatatcgt ttcactgcgt gatgtgattg 7620
agagtgataa atcgactctg ctcggagagg ccgttgccaa acgctttggc gaactgcctt 7680
tcctgttcaa agtattatgc gcagcacagc cactctccat tcaggttcat ccaaacaaac 7740
acaattctga aatcggtttt gccaaagaaa atgccgcagg tatcccgatg gatgccgccg 7800
agcgtaacta taaagatcct aaccacaagc cggagctggt ttttgcgctg acgcctttcc 7860
ttgcgatgaa cgcgtttcgt gaattttccg agattgtctc cctactccag ccggtcgcag 7920
gtgcacatcc ggcgattgct cactttttac aacagcctga tgccgaacgt ttaagcgaac 7980
tgttcgccag cctgttgaat atgcagggtg aagaaaaatc ccgcgcgctg gcgattttaa 8040
aatcggccct cgatagccag cagggtgaac cgtggcaaac gattcgttta atttctgaat 8100
tttacccgga agacagcggt ctgttctccc cgctattgct gaatgtggtg aaattgaacc 8160
ctggcgaagc gatgttcctg ttcgctgaaa caccgcacgc ttacctgcaa ggcgtggcgc 8220
tggaagtgat ggcaaactcc gataacgtgc tgcgtgcggg tctgacgcct aaatacattg 8280
atattccgga actggttgcc aatgtgaaat tcgaagccaa accggctaac cagttgttga 8340
cccagccggt gaaacaaggt gcagaactgg acttcccgat tccagtggat gattttgcct 8400
tctcgctgca tgaccttagt gataaagaaa ccaccattag ccagcagagt gccgccattt 8460
tgttctgcgt cgaaggcgat gcaacgttgt ggaaaggttc tcagcagtta cagcttaaac 8520
cgggtgaatc agcgtttatt gccgccaacg aatcaccggt gactgtcaaa ggccacggcc 8580
gtttagcgcg tgtttacaac aagctgtaag agcttactga aaaaattaac atctcttgct 8640
aagctgggtc atgggtcgtt taagctgccg atgtgcctgc gtcgtctggt gccctctctc 8700
catatggagg ttgtcaaagt atctgctgtt cgtgtcatga gtcgtgtcag tgttggttta 8760
ataatggacc ggttgtgttg tgtgtgcgta ctacccagaa ctatgacaaa tcatgaataa 8820
gtttgatgtt tgaaattaaa gcctgtgctc attatgttct gtctttcagt tgtctcctaa 8880
tatttgcctc caggtactgg ctatctaccg tttcttactt aggaggtgtt tgaatgcact 8940
aaaactaata gttagtggct aaaattagtt aaaacatcca aacaccatag ctaatagttg 9000
aactattagc tatttttgga aaattagtta atagtgaggt agttatttgt tagctagcta 9060
attcaactaa caatttttag ccaactaaca attagtttca gtgcattcaa acaccccctt 9120
aatgttaacg tggttctatc taccgtctcc taatatatgg ttgattgttc ggtttgttgc 9180
tatgctattg ggttctgatt gctgctagtt cttgctgaat ccagaagttc tcgtagtata 9240
gctcagattc atattattta tttgagtgat aagtgatcca ggttattact atgttagcta 9300
ggtttttttt acaaggataa attatctgtg atcataattc ttatgaaagc tttatgtttc 9360
ctggaggcag tggcatgcaa tgcatgacag caacttgatc acaccagctg aggtagatac 9420
ggtaacaagg ttcttaaatc tgttcaccaa atcattggag aacacacata cacattcttg 9480
ccagtcttgg ttagagaaat ttcatgacaa aatgccaaag ctgtcttgac tcttcacttt 9540
tggccatgag tcgtgactta gtttggttta atggaccggt tctcctagct tgttctactc 9600
aaaactgttg ttgatgcgaa taagttgtga tggttgatct ctggattttg ttttgctctc 9660
aatagtggac gagattagat agcctgcagg cccgggggcg cgccctaatt agctaacggc 9720
caggatcgcc gcgtgagcct ttagcaacta gctagattaa ttaacgcaat ctgttattaa 9780
gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc 9840
tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca tcagaattaa 9900
ttctcatgtt tgacagctta tcatcgactg cacggtgcac caatgcttct ggcgtcaggc 9960
agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc 10020
tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca 10080
aatattctga aatgagctgt tgacaattaa tcatccggct cgtataatgt gtggaattgt 10140
gagcggataa caatttcaca caggaaacag accatgaggg aagcgttgat cgccgaagta 10200
tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg 10260
gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat 10320
ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac 10380
cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc 10440
attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt 10500
ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt 10560
gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg 10620
gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc 10680
ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg 10740
ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc 10800
gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctaggcag 10860
gcttatcttg gacaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt 10920
cactacgtga aaggcgagat caccaaagta gtcggcaaat aaagctctag tggatctccg 10980
tacccaggga tctggctcgc ggcggacgca cgacgccggg gcgagaccat aggcgatctc 11040
ctaaatcaat agtagctgta acctcgaagc gtttcacttg taacaacgat tgagaatttt 11100
tgtcataaaa ttgaaatact tggttcgcat ttttgtcatc cgcggtcagc cgcaattctg 11160
acgaactgcc catttagctg gagatgattg tacatccttc acgtgaaaat ttctcaagcg 11220
ctgtgaacaa gggttcagat tttagattga aaggtgagcc gttgaaacac gttcttcttg 11280
tcgatgacga cgtcgctatg cggcatctta ttattgaata ccttacgatc cacgccttca 11340
aagtgaccgc ggtagccgac agcacccagt tcacaagagt actctcttcc gcgacggtcg 11400
atgtcgtggt tgttgatcta gatttaggtc gtgaagatgg gctcgagatc gttcgtaatc 11460
tggcggcaaa gtctgatatt ccaatcataa ttatcagtgg cgaccgcctt gaggagacgg 11520
ataaagttgt tgcactcgag ctaggagcaa gtgattttat cgctaagccg ttcagtatca 11580
gagagtttct agcacgcatt cgggttgcct tgcgcgtgcg ccccaacgtt gtccgctcca 11640
aagaccgacg gtctttttgt tttactgact ggacacttaa tctcaggcaa cgtcgcttga 11700
tgtccgaagc tggcggtgag gtgaaactta cggcaggtga gttcaatctt ctcctcgcgt 11760
ttttagagaa accccgcgac gttctatcgc gcgagcaact tctcattgcc agtcgagtac 11820
gcgacgagga ggtttatgac aggagtatag atgttctcat tttgaggctg cgccgcaaac 11880
ttgaggcaga tccgtcaagc cctcaactga taaaaacagc aagaggtgcc ggttatttct 11940
ttgacgcgga cgtgcaggtt tcgcacgggg ggacgatggc agcctgagcc aattcccaga 12000
tccccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc 12060
gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat 12120
cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga 12180
atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga 12240
gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat 12300
catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg 12360
ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt 12420
gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata 12480
ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact 12540
caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat 12600
tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct 12660
ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac 12720
cgggcggccg gagtacatcg agatcgagct ggctgattgg atgtaccgcg agatcacaga 12780
aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat 12840
cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt 12900
gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac 12960
cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg 13020
gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc 13080
cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg 13140
aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa 13200
ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt 13260
gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa 13320
aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct 13380
gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc 13440
tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg 13500
cggacaagcc gcgccgtcgc cactcgaccg ccggcgctga ggtctgcctc gtgaagaagg 13560
tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag tgagggagcc 13620
acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact tttgctttgc 13680
cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact cagcaaaagt 13740
tcgatttatt caacaaagcc gccgtcccgt caagtcagcg taatgctctg ccagtgttac 13800
aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta 13860
ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa 13920
aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact 13980
cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag 14040
aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagctctgc attaatgaat 14100
cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 14160
tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 14220
aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 14280
gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 14340
ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 14400
ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 14460
gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 14520
ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 14580
cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 14640
cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 14700
gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 14760
aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 14820
tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 14880
gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 14940
tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 15000
gatcttcacc tagatccttt tgatccggaa tta 15033
<210> 60
<211> 940
<212> PRT
<213> 人工序列
<220>
<223> 突变型Cry1Ab
<400> 60
Met Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu
1 5 10 15
Ser Asn Pro Glu Val Glu Val Leu Gly Gly Glu Arg Ile Glu Thr Gly
20 25 30
Tyr Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser
35 40 45
Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile
50 55 60
Trp Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile
65 70 75 80
Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala
85 90 95
Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr Ala Glu
100 105 110
Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu
115 120 125
Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Thr Thr Ala
130 135 140
Ile Pro Leu Phe Ala Val Gln Asn Tyr Gln Val Pro Leu Leu Ser Val
145 150 155 160
Tyr Val Gln Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser
165 170 175
Val Phe Gly Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg
180 185 190
Tyr Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp His Ala Val
195 200 205
Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg
210 215 220
Asp Trp Ile Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val
225 230 235 240
Leu Asp Ile Val Ser Leu Phe Pro Asn Tyr Asp Ser Arg Thr Tyr Pro
245 250 255
Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr Thr Asn Pro Val
260 265 270
Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu
275 280 285
Gly Ser Ile Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr
290 295 300
Ile Tyr Thr Asp Ala His Arg Gly Glu Tyr Tyr Trp Ser Gly His Gln
305 310 315 320
Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro
325 330 335
Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile Val Ala
340 345 350
Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Leu Tyr Arg
355 360 365
Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val Leu Asp
370 375 380
Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu Pro Ser Ala Val
385 390 395 400
Tyr Arg Lys Ser Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln
405 410 415
Asn Asn Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His
420 425 430
Val Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile
435 440 445
Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn
450 455 460
Ile Ile Pro Ser Ser Gln Ile Thr Gln Ile Pro Leu Thr Lys Ser Thr
465 470 475 480
Asn Leu Gly Ser Gly Thr Ser Val Val Lys Gly Pro Gly Phe Thr Gly
485 490 495
Gly Asp Ile Leu Arg Arg Thr Ser Pro Gly Gln Ile Ser Thr Leu Arg
500 505 510
Val Asn Ile Thr Ala Pro Leu Ser Gln Arg Tyr Arg Val Arg Ile Arg
515 520 525
Tyr Ala Ser Thr Thr Asn Leu Gln Phe His Thr Ser Ile Asp Gly Arg
530 535 540
Pro Ile Asn Gln Gly Asn Phe Ser Ala Thr Met Ser Ser Gly Ser Asn
545 550 555 560
Leu Gln Ser Gly Ser Phe Arg Thr Val Gly Phe Thr Thr Pro Phe Asn
565 570 575
Phe Ser Asn Gly Ser Ser Val Phe Thr Leu Ser Ala His Val Phe Asn
580 585 590
Ser Gly Asn Glu Val Tyr Ile Asp Arg Ile Glu Phe Val Pro Ala Glu
595 600 605
Val Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val
610 615 620
Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val
625 630 635 640
Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser
645 650 655
Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys
660 665 670
His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn
675 680 685
Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr
690 695 700
Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val
705 710 715 720
Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln
725 730 735
Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg
740 745 750
Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr
755 760 765
Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp
770 775 780
Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Thr Ser His His Ser
785 790 795 800
His His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu
805 810 815
Asp Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His
820 825 830
Ala Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu Val Gly
835 840 845
Glu Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys
850 855 860
Arg Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys
865 870 875 880
Glu Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln
885 890 895
Ala Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg Val His
900 905 910
Ser Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val
915 920 925
Asn Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile
930 935 940
<210> 61
<211> 959
<212> PRT
<213> 人工序列
<220>
<223> 突变型Cry1Ab
<400> 61
Met Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu
1 5 10 15
Ser Asn Pro Glu Val Glu Val Leu Gly Gly Glu Arg Ile Glu Thr Gly
20 25 30
Tyr Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser
35 40 45
Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile
50 55 60
Trp Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile
65 70 75 80
Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala
85 90 95
Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr Ala Glu
100 105 110
Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu
115 120 125
Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Thr Thr Ala
130 135 140
Ile Pro Leu Phe Ala Val Gln Asn Tyr Gln Val Pro Leu Leu Ser Val
145 150 155 160
Tyr Val Gln Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser
165 170 175
Val Phe Gly Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg
180 185 190
Tyr Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp His Ala Val
195 200 205
Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg
210 215 220
Asp Trp Ile Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val
225 230 235 240
Leu Asp Ile Val Ser Leu Phe Pro Asn Tyr Asp Ser Arg Thr Tyr Pro
245 250 255
Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr Thr Asn Pro Val
260 265 270
Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu
275 280 285
Gly Ser Ile Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr
290 295 300
Ile Tyr Thr Asp Ala His Arg Gly Glu Tyr Tyr Trp Ser Gly His Gln
305 310 315 320
Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro
325 330 335
Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile Val Ala
340 345 350
Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Leu Tyr Arg
355 360 365
Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val Leu Asp
370 375 380
Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu Pro Ser Ala Val
385 390 395 400
Tyr Arg Lys Ser Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln
405 410 415
Asn Asn Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His
420 425 430
Val Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile
435 440 445
Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn
450 455 460
Ile Ile Pro Ser Ser Gln Ile Thr Gln Ile Pro Leu Thr Lys Ser Thr
465 470 475 480
Asn Leu Gly Ser Gly Thr Ser Val Val Lys Gly Pro Gly Phe Thr Gly
485 490 495
Gly Asp Ile Leu Arg Arg Thr Ser Pro Gly Gln Ile Ser Thr Leu Arg
500 505 510
Val Asn Ile Thr Ala Pro Leu Ser Gln Arg Tyr Arg Val Arg Ile Arg
515 520 525
Tyr Ala Ser Thr Thr Asn Leu Gln Phe His Thr Ser Ile Asp Gly Arg
530 535 540
Pro Ile Asn Gln Gly Asn Phe Ser Ala Thr Met Ser Ser Gly Ser Asn
545 550 555 560
Leu Gln Ser Gly Ser Phe Arg Thr Val Gly Phe Thr Thr Pro Phe Asn
565 570 575
Phe Ser Asn Gly Ser Ser Val Phe Thr Leu Ser Ala His Val Phe Asn
580 585 590
Ser Gly Asn Glu Val Tyr Ile Asp Arg Ile Glu Phe Val Pro Ala Glu
595 600 605
Val Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val
610 615 620
Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val
625 630 635 640
Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser
645 650 655
Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys
660 665 670
His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn
675 680 685
Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr
690 695 700
Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val
705 710 715 720
Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln
725 730 735
Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg
740 745 750
Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr
755 760 765
Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp
770 775 780
Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Thr Ser Ile Leu Phe
785 790 795 800
Ile Asp Lys Lys Ser Thr Val His Ser Asn Cys Lys Leu Val Ala Ser
805 810 815
His His Ser His His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp
820 825 830
Leu Asn Glu Asp Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln
835 840 845
Asp Gly His Ala Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro
850 855 860
Leu Val Gly Glu Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp
865 870 875 880
Arg Asp Lys Arg Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys
885 890 895
Glu Ala Lys Glu Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp
900 905 910
Arg Leu Gln Ala Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys
915 920 925
Arg Val His Ser Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile
930 935 940
Pro Gly Val Asn Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile
945 950 955
<210> 62
<211> 959
<212> PRT
<213> 人工序列
<220>
<223> 突变型Cry1Ab
<400> 62
Met Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu
1 5 10 15
Ser Asn Pro Glu Val Glu Val Leu Gly Gly Glu Arg Ile Glu Thr Gly
20 25 30
Tyr Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser
35 40 45
Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile
50 55 60
Trp Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile
65 70 75 80
Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala
85 90 95
Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr Ala Glu
100 105 110
Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu
115 120 125
Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Thr Thr Ala
130 135 140
Ile Pro Leu Phe Ala Val Gln Asn Tyr Gln Val Pro Leu Leu Ser Val
145 150 155 160
Tyr Val Gln Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser
165 170 175
Val Phe Gly Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg
180 185 190
Tyr Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp His Ala Val
195 200 205
Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg
210 215 220
Asp Trp Ile Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val
225 230 235 240
Leu Asp Ile Val Ser Leu Phe Pro Asn Tyr Asp Ser Arg Thr Tyr Pro
245 250 255
Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr Thr Asn Pro Val
260 265 270
Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu
275 280 285
Gly Ser Ile Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr
290 295 300
Ile Tyr Thr Asp Ala His Arg Gly Glu Tyr Tyr Trp Ser Gly His Gln
305 310 315 320
Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro
325 330 335
Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile Val Ala
340 345 350
Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Leu Tyr Arg
355 360 365
Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val Leu Asp
370 375 380
Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu Pro Ser Ala Val
385 390 395 400
Tyr Arg Lys Ser Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln
405 410 415
Asn Asn Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His
420 425 430
Val Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile
435 440 445
Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn
450 455 460
Ile Ile Pro Ser Ser Gln Ile Thr Gln Ile Pro Leu Thr Lys Ser Thr
465 470 475 480
Asn Leu Gly Ser Gly Thr Ser Val Val Lys Gly Pro Gly Phe Thr Gly
485 490 495
Gly Asp Ile Leu Arg Arg Thr Ser Pro Gly Gln Ile Ser Thr Leu Arg
500 505 510
Val Asn Ile Thr Ala Pro Leu Ser Gln Arg Tyr Arg Val Arg Ile Arg
515 520 525
Tyr Ala Ser Thr Thr Asn Leu Gln Phe His Thr Ser Ile Asp Gly Arg
530 535 540
Pro Ile Asn Gln Gly Asn Phe Ser Ala Thr Met Ser Ser Gly Ser Asn
545 550 555 560
Leu Gln Ser Gly Ser Phe Arg Thr Val Gly Phe Thr Thr Pro Phe Asn
565 570 575
Phe Ser Asn Gly Ser Ser Val Phe Thr Leu Ser Ala His Val Phe Asn
580 585 590
Ser Gly Asn Glu Val Tyr Ile Asp Arg Ile Glu Phe Val Pro Ala Glu
595 600 605
Val Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val
610 615 620
Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val
625 630 635 640
Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser
645 650 655
Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys
660 665 670
His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn
675 680 685
Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr
690 695 700
Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val
705 710 715 720
Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln
725 730 735
Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg
740 745 750
Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr
755 760 765
Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp
770 775 780
Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Thr Ser Thr Ser Asn
785 790 795 800
Thr His Glu Met Thr Asp Arg Ala Val Ser Arg Pro Ser Leu Thr Ser
805 810 815
His His Ser His His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp
820 825 830
Leu Asn Glu Asp Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln
835 840 845
Asp Gly His Ala Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro
850 855 860
Leu Val Gly Glu Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp
865 870 875 880
Arg Asp Lys Arg Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys
885 890 895
Glu Ala Lys Glu Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp
900 905 910
Arg Leu Gln Ala Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys
915 920 925
Arg Val His Ser Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile
930 935 940
Pro Gly Val Asn Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile
945 950 955
<210> 63
<211> 958
<212> PRT
<213> 人工序列
<220>
<223> 突变型Cry1Ab
<400> 63
Met Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu
1 5 10 15
Ser Asn Pro Glu Val Glu Val Leu Gly Gly Glu Arg Ile Glu Thr Gly
20 25 30
Tyr Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser
35 40 45
Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile
50 55 60
Trp Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile
65 70 75 80
Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala
85 90 95
Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr Ala Glu
100 105 110
Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu
115 120 125
Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Thr Thr Ala
130 135 140
Ile Pro Leu Phe Ala Val Gln Asn Tyr Gln Val Pro Leu Leu Ser Val
145 150 155 160
Tyr Val Gln Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser
165 170 175
Val Phe Gly Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg
180 185 190
Tyr Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp His Ala Val
195 200 205
Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg
210 215 220
Asp Trp Ile Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val
225 230 235 240
Leu Asp Ile Val Ser Leu Phe Pro Asn Tyr Asp Ser Arg Thr Tyr Pro
245 250 255
Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr Thr Asn Pro Val
260 265 270
Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu
275 280 285
Gly Ser Ile Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr
290 295 300
Ile Tyr Thr Asp Ala His Arg Gly Glu Tyr Tyr Trp Ser Gly His Gln
305 310 315 320
Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro
325 330 335
Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile Val Ala
340 345 350
Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Leu Tyr Arg
355 360 365
Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val Leu Asp
370 375 380
Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu Pro Ser Ala Val
385 390 395 400
Tyr Arg Lys Ser Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln
405 410 415
Asn Asn Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His
420 425 430
Val Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile
435 440 445
Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn
450 455 460
Ile Ile Pro Ser Ser Gln Ile Thr Gln Ile Pro Leu Thr Lys Ser Thr
465 470 475 480
Asn Leu Gly Ser Gly Thr Ser Val Val Lys Gly Pro Gly Phe Thr Gly
485 490 495
Gly Asp Ile Leu Arg Arg Thr Ser Pro Gly Gln Ile Ser Thr Leu Arg
500 505 510
Val Asn Ile Thr Ala Pro Leu Ser Gln Arg Tyr Arg Val Arg Ile Arg
515 520 525
Tyr Ala Ser Thr Thr Asn Leu Gln Phe His Thr Ser Ile Asp Gly Arg
530 535 540
Pro Ile Asn Gln Gly Asn Phe Ser Ala Thr Met Ser Ser Gly Ser Asn
545 550 555 560
Leu Gln Ser Gly Ser Phe Arg Thr Val Gly Phe Thr Thr Pro Phe Asn
565 570 575
Phe Ser Asn Gly Ser Ser Val Phe Thr Leu Ser Ala His Val Phe Asn
580 585 590
Ser Gly Asn Glu Val Tyr Ile Asp Arg Ile Glu Phe Val Pro Ala Glu
595 600 605
Val Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val
610 615 620
Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val
625 630 635 640
Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser
645 650 655
Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys
660 665 670
His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn
675 680 685
Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr
690 695 700
Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val
705 710 715 720
Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln
725 730 735
Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg
740 745 750
Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr
755 760 765
Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp
770 775 780
Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Thr Ser Ile Ser Val
785 790 795 800
Ala Phe Leu Ile Glu Pro Cys Gln Gln Leu Val Gly Phe Thr Ser His
805 810 815
His Ser His His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu
820 825 830
Asn Glu Asp Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp
835 840 845
Gly His Ala Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu
850 855 860
Val Gly Glu Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg
865 870 875 880
Asp Lys Arg Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu
885 890 895
Ala Lys Glu Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg
900 905 910
Leu Gln Ala Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg
915 920 925
Val His Ser Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro
930 935 940
Gly Val Asn Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile
945 950 955
<210> 64
<211> 959
<212> PRT
<213> 人工序列
<220>
<223> 突变型Cry1Ab
<400> 64
Met Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu
1 5 10 15
Ser Asn Pro Glu Val Glu Val Leu Gly Gly Glu Arg Ile Glu Thr Gly
20 25 30
Tyr Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser
35 40 45
Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile
50 55 60
Trp Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile
65 70 75 80
Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala
85 90 95
Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr Ala Glu
100 105 110
Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu
115 120 125
Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Thr Thr Ala
130 135 140
Ile Pro Leu Phe Ala Val Gln Asn Tyr Gln Val Pro Leu Leu Ser Val
145 150 155 160
Tyr Val Gln Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser
165 170 175
Val Phe Gly Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg
180 185 190
Tyr Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp His Ala Val
195 200 205
Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg
210 215 220
Asp Trp Ile Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val
225 230 235 240
Leu Asp Ile Val Ser Leu Phe Pro Asn Tyr Asp Ser Arg Thr Tyr Pro
245 250 255
Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr Thr Asn Pro Val
260 265 270
Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu
275 280 285
Gly Ser Ile Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr
290 295 300
Ile Tyr Thr Asp Ala His Arg Gly Glu Tyr Tyr Trp Ser Gly His Gln
305 310 315 320
Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro
325 330 335
Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile Val Ala
340 345 350
Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Leu Tyr Arg
355 360 365
Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val Leu Asp
370 375 380
Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu Pro Ser Ala Val
385 390 395 400
Tyr Arg Lys Ser Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln
405 410 415
Asn Asn Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His
420 425 430
Val Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile
435 440 445
Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn
450 455 460
Ile Ile Pro Ser Ser Gln Ile Thr Gln Ile Pro Leu Thr Lys Ser Thr
465 470 475 480
Asn Leu Gly Ser Gly Thr Ser Val Val Lys Gly Pro Gly Phe Thr Gly
485 490 495
Gly Asp Ile Leu Arg Arg Thr Ser Pro Gly Gln Ile Ser Thr Leu Arg
500 505 510
Val Asn Ile Thr Ala Pro Leu Ser Gln Arg Tyr Arg Val Arg Ile Arg
515 520 525
Tyr Ala Ser Thr Thr Asn Leu Gln Phe His Thr Ser Ile Asp Gly Arg
530 535 540
Pro Ile Asn Gln Gly Asn Phe Ser Ala Thr Met Ser Ser Gly Ser Asn
545 550 555 560
Leu Gln Ser Gly Ser Phe Arg Thr Val Gly Phe Thr Thr Pro Phe Asn
565 570 575
Phe Ser Asn Gly Ser Ser Val Phe Thr Leu Ser Ala His Val Phe Asn
580 585 590
Ser Gly Asn Glu Val Tyr Ile Asp Arg Ile Glu Phe Val Pro Ala Glu
595 600 605
Val Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val
610 615 620
Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val
625 630 635 640
Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser
645 650 655
Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys
660 665 670
His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn
675 680 685
Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr
690 695 700
Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val
705 710 715 720
Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln
725 730 735
Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg
740 745 750
Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr
755 760 765
Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp
770 775 780
Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Thr Ser Asp Asp Pro
785 790 795 800
Lys Gly Gln Val Ser His Ser Phe Ile Glu Gln Leu Phe Ala Ser Ser
805 810 815
His His Ser His His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp
820 825 830
Leu Asn Glu Asp Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln
835 840 845
Asp Gly His Ala Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro
850 855 860
Leu Val Gly Glu Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp
865 870 875 880
Arg Asp Lys Arg Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys
885 890 895
Glu Ala Lys Glu Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp
900 905 910
Arg Leu Gln Ala Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys
915 920 925
Arg Val His Ser Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile
930 935 940
Pro Gly Val Asn Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile
945 950 955
<210> 65
<211> 958
<212> PRT
<213> 人工序列
<220>
<223> 突变型Cry1Ab
<400> 65
Met Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu
1 5 10 15
Ser Asn Pro Glu Val Glu Val Leu Gly Gly Glu Arg Ile Glu Thr Gly
20 25 30
Tyr Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser
35 40 45
Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile
50 55 60
Trp Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile
65 70 75 80
Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala
85 90 95
Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr Ala Glu
100 105 110
Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu
115 120 125
Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Thr Thr Ala
130 135 140
Ile Pro Leu Phe Ala Val Gln Asn Tyr Gln Val Pro Leu Leu Ser Val
145 150 155 160
Tyr Val Gln Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser
165 170 175
Val Phe Gly Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg
180 185 190
Tyr Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp His Ala Val
195 200 205
Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg
210 215 220
Asp Trp Ile Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val
225 230 235 240
Leu Asp Ile Val Ser Leu Phe Pro Asn Tyr Asp Ser Arg Thr Tyr Pro
245 250 255
Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr Thr Asn Pro Val
260 265 270
Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu
275 280 285
Gly Ser Ile Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr
290 295 300
Ile Tyr Thr Asp Ala His Arg Gly Glu Tyr Tyr Trp Ser Gly His Gln
305 310 315 320
Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro
325 330 335
Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile Val Ala
340 345 350
Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Leu Tyr Arg
355 360 365
Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val Leu Asp
370 375 380
Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu Pro Ser Ala Val
385 390 395 400
Tyr Arg Lys Ser Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln
405 410 415
Asn Asn Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His
420 425 430
Val Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile
435 440 445
Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn
450 455 460
Ile Ile Pro Ser Ser Gln Ile Thr Gln Ile Pro Leu Thr Lys Ser Thr
465 470 475 480
Asn Leu Gly Ser Gly Thr Ser Val Val Lys Gly Pro Gly Phe Thr Gly
485 490 495
Gly Asp Ile Leu Arg Arg Thr Ser Pro Gly Gln Ile Ser Thr Leu Arg
500 505 510
Val Asn Ile Thr Ala Pro Leu Ser Gln Arg Tyr Arg Val Arg Ile Arg
515 520 525
Tyr Ala Ser Thr Thr Asn Leu Gln Phe His Thr Ser Ile Asp Gly Arg
530 535 540
Pro Ile Asn Gln Gly Asn Phe Ser Ala Thr Met Ser Ser Gly Ser Asn
545 550 555 560
Leu Gln Ser Gly Ser Phe Arg Thr Val Gly Phe Thr Thr Pro Phe Asn
565 570 575
Phe Ser Asn Gly Ser Ser Val Phe Thr Leu Ser Ala His Val Phe Asn
580 585 590
Ser Gly Asn Glu Val Tyr Ile Asp Arg Ile Glu Phe Val Pro Ala Glu
595 600 605
Val Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val
610 615 620
Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val
625 630 635 640
Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser
645 650 655
Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys
660 665 670
His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn
675 680 685
Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr
690 695 700
Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val
705 710 715 720
Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln
725 730 735
Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg
740 745 750
Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr
755 760 765
Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp
770 775 780
Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Thr Ser Pro Phe Ser
785 790 795 800
Leu Phe Leu Ser Leu Cys Phe Ile Glu Leu Leu Lys Val Thr Ser His
805 810 815
His Ser His His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu
820 825 830
Asn Glu Asp Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp
835 840 845
Gly His Ala Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu
850 855 860
Val Gly Glu Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg
865 870 875 880
Asp Lys Arg Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu
885 890 895
Ala Lys Glu Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg
900 905 910
Leu Gln Ala Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg
915 920 925
Val His Ser Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro
930 935 940
Gly Val Asn Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile
945 950 955
<210> 66
<211> 940
<212> PRT
<213> 人工序列
<220>
<223> 突变型Cry1Ab
<400> 66
Met Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu
1 5 10 15
Ser Asn Pro Glu Val Glu Val Leu Gly Gly Glu Arg Ile Glu Thr Gly
20 25 30
Tyr Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser
35 40 45
Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile
50 55 60
Trp Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile
65 70 75 80
Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala
85 90 95
Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr Ala Glu
100 105 110
Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu
115 120 125
Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Thr Thr Ala
130 135 140
Ile Pro Leu Phe Ala Val Gln Asn Tyr Gln Val Pro Leu Leu Ser Val
145 150 155 160
Tyr Val Gln Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser
165 170 175
Val Phe Gly Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg
180 185 190
Tyr Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp His Ala Val
195 200 205
Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg
210 215 220
Asp Trp Ile Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val
225 230 235 240
Leu Asp Ile Val Ser Leu Phe Pro Asn Tyr Asp Ser Arg Thr Tyr Pro
245 250 255
Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr Thr Asn Pro Val
260 265 270
Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu
275 280 285
Gly Ser Ile Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr
290 295 300
Ile Tyr Thr Asp Ala His Arg Gly Glu Tyr Tyr Trp Ser Gly His Gln
305 310 315 320
Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro
325 330 335
Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile Val Ala
340 345 350
Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Leu Tyr Arg
355 360 365
Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val Leu Asp
370 375 380
Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu Pro Ser Ala Val
385 390 395 400
Tyr Arg Lys Ser Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln
405 410 415
Asn Asn Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His
420 425 430
Val Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile
435 440 445
Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn
450 455 460
Ile Ile Pro Ser Ser Gln Ile Thr Gln Ile Pro Leu Thr Lys Ser Thr
465 470 475 480
Asn Leu Gly Ser Gly Thr Ser Val Val Lys Gly Pro Gly Phe Thr Gly
485 490 495
Gly Asp Ile Leu Arg Arg Thr Ser Pro Gly Gln Ile Ser Thr Leu Arg
500 505 510
Val Asn Ile Thr Ala Pro Leu Ser Gln Arg Tyr Arg Val Arg Ile Arg
515 520 525
Tyr Ala Ser Thr Thr Asn Leu Gln Phe His Thr Ser Ile Asp Gly Arg
530 535 540
Pro Ile Asn Gln Gly Asn Phe Ser Ala Thr Met Ser Ser Gly Ser Asn
545 550 555 560
Leu Gln Ser Gly Ser Phe Arg Thr Val Gly Phe Thr Thr Pro Phe Asn
565 570 575
Phe Ser Asn Gly Ser Ser Val Phe Thr Leu Ser Ala His Val Phe Asn
580 585 590
Ser Gly Asn Glu Val Tyr Ile Asp Arg Ile Glu Phe Val Pro Ala Glu
595 600 605
Val Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val
610 615 620
Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val
625 630 635 640
Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser
645 650 655
Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys
660 665 670
His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn
675 680 685
Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr
690 695 700
Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val
705 710 715 720
Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln
725 730 735
Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg
740 745 750
Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr
755 760 765
Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp
770 775 780
Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Thr Ser His His Ser
785 790 795 800
His His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu
805 810 815
Asp Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His
820 825 830
Ala Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu Val Gly
835 840 845
Glu Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys
850 855 860
Arg Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys
865 870 875 880
Glu Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln
885 890 895
Ala Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg Val His
900 905 910
Ser Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val
915 920 925
Asn Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile
930 935 940
<210> 67
<211> 2823
<212> DNA
<213> 人工序列
<220>
<223> 突变型cry1Ab
<400> 67
atggacaaca accccaacat caacgagtgc atcccctaca actgcctgag caaccccgag 60
gtggaggtgc tgggcggcga gcgcatcgag accggctaca cccccatcga catcagcctg 120
agcctgaccc agttcctgct gagcgagttc gtgcccggcg ccggcttcgt gctgggcctg 180
gtggacatca tctggggcat cttcggcccc agccagtggg acgccttcct ggtgcagatc 240
gagcagttga taaaccaacg catagaggaa ttcgcccgca accaggccat cagccgcctg 300
gagggcctga gcaacctgta ccaaatctac gccgagagct tccgcgagtg ggaggccgac 360
cccaccaacc ccgccctgcg cgaggagatg cgcatccagt tcaacgacat gaacagcgcc 420
ctgaccaccg ccatccccct gttcgccgtg cagaactacc aggtgcccct gctgagcgtg 480
tacgtgcagg ccgccaacct gcacctgagc gtgctgcgcg acgtcagcgt gttcggccag 540
cgctggggct tcgacgccgc caccatcaac agccgctaca acgacctgac ccgcctgatc 600
ggcaactaca ccgaccacgc cgtgcgctgg tacaacaccg gcctggagcg cgtgtggggg 660
cccgacagcc gcgactggat caggtacaac cagttccgcc gcgagctgac cctgaccgtg 720
ctggacatcg tgagcctgtt ccccaactac gacagccgca cctaccccat ccgcaccgtg 780
agccagctga cccgcgagat ttacaccaac cccgtgctgg agaacttcga cggcagcttc 840
cgcggcagcg cccagggcat cgagggcagc atccgcagcc cccacctgat ggacatcctg 900
aacagcatca ccatctacac cgacgcccac cgcggcgagt actactggag cggccaccag 960
atcatggcca gccccgtcgg cttcagcggc cccgagttca ccttccccct gtacggcacg 1020
atgggcaacg ctgcacctca gcagcgcatc gtggcacagc tgggccaggg agtgtaccgc 1080
accctgagca gcaccctgta ccgtcgacct ttcaacatcg gcatcaacaa ccagcagctg 1140
agcgtgctgg acggcaccga gttcgcctac ggcaccagca gcaacctgcc cagcgccgtg 1200
taccgcaaga gcggcaccgt ggacagcctg gacgagatcc cccctcagaa caacaacgtg 1260
ccacctcgac agggcttcag ccaccgtctg agccacgtga gcatgttccg cagtggcttc 1320
agcaacagca gcgtgagcat catccgtgca cctatgttca gctggattca ccgcagtgcc 1380
gagttcaaca acatcatccc cagcagccag atcacccaga tccccctgac caagagcacc 1440
aacctgggca gcggcaccag cgtggtgaag ggccccggct tcaccggcgg cgacatcctg 1500
cgccgcacca gccccggcca gatcagcacc ctgcgcgtga acatcaccgc ccccctgagc 1560
cagcgctacc gcgtccgcat ccgctacgcc agcaccacca acctgcagtt ccacaccagc 1620
atcgacggcc gccccatcaa ccagggcaac ttcagcgcca ccatgagcag cggcagcaac 1680
ctgcagagcg gcagcttccg caccgtgggc ttcaccaccc ccttcaactt cagcaacggc 1740
agcagcgtgt tcaccctgag cgcccacgtg ttcaacagcg gcaacgaggt gtacatcgac 1800
cgcatcgagt tcgtgcccgc cgaggtgacc ttcgaggccg agtacgacct ggagagggct 1860
cagaaggccg tgaacgagct gttcaccagc agcaaccaga tcggcctgaa gaccgacgtg 1920
accgactacc acatcgacca ggtgagcaac ctggtggagt gcttaagcga cgagttctgc 1980
ctggacgaga agaaggagct gagcgagaag gtgaagcacg ccaagcgcct gagcgacgag 2040
cgcaacctgc tgcaggaccc caacttccgc ggcatcaacc gccagctgga ccgcggctgg 2100
cgaggcagca ccgatatcac catccagggc ggcgacgacg tgttcaagga gaactacgtg 2160
accctgctgg gcaccttcga cgagtgctac cccacctacc tgtaccagaa gatcgacgag 2220
agcaagctga aggcctacac ccgctaccag ctgcgcggct acatcgagga cagccaggac 2280
ctggaaatct acctgatccg ctacaacgcg aagcacgaga ccgtgaacgt gcccggcacc 2340
ggcagcctgt ggcccctgag cgcccccagc cccatcggca agtgcactag tcaccacagc 2400
caccacttca gcctggacat cgacgtgggc tgcaccgacc tgaacgagga cctgggcgtg 2460
tgggtgatct tcaagatcaa gacccaggac ggccacgccc gcctgggcaa tctagagttc 2520
ctggaggaga agcccctggt gggcgaggcc ctggcccgcg tgaagcgtgc tgagaagaag 2580
tggcgcgaca agcgcgagaa gctggagtgg gagaccaaca tcgtgtacaa ggaggccaag 2640
gagagcgtgg acgccctgtt cgtgaacagc cagtacgacc gcctgcaggc cgacaccaac 2700
atcgccatga tccacgccgc cgacaagcgc gtgcacagca ttcgcgaggc ctacctgccc 2760
gagctgagcg tgatccccgg tgtgaacgcc gccatcttcg aggaactcga gggccgcatc 2820
tag 2823
<210> 68
<211> 2880
<212> DNA
<213> 人工序列
<220>
<223> 突变型cry1Ab
<400> 68
atggacaaca accccaacat caacgagtgc atcccctaca actgcctgag caaccccgag 60
gtggaggtgc tgggcggcga gcgcatcgag accggctaca cccccatcga catcagcctg 120
agcctgaccc agttcctgct gagcgagttc gtgcccggcg ccggcttcgt gctgggcctg 180
gtggacatca tctggggcat cttcggcccc agccagtggg acgccttcct ggtgcagatc 240
gagcagttga taaaccaacg catagaggaa ttcgcccgca accaggccat cagccgcctg 300
gagggcctga gcaacctgta ccaaatctac gccgagagct tccgcgagtg ggaggccgac 360
cccaccaacc ccgccctgcg cgaggagatg cgcatccagt tcaacgacat gaacagcgcc 420
ctgaccaccg ccatccccct gttcgccgtg cagaactacc aggtgcccct gctgagcgtg 480
tacgtgcagg ccgccaacct gcacctgagc gtgctgcgcg acgtcagcgt gttcggccag 540
cgctggggct tcgacgccgc caccatcaac agccgctaca acgacctgac ccgcctgatc 600
ggcaactaca ccgaccacgc cgtgcgctgg tacaacaccg gcctggagcg cgtgtggggg 660
cccgacagcc gcgactggat caggtacaac cagttccgcc gcgagctgac cctgaccgtg 720
ctggacatcg tgagcctgtt ccccaactac gacagccgca cctaccccat ccgcaccgtg 780
agccagctga cccgcgagat ttacaccaac cccgtgctgg agaacttcga cggcagcttc 840
cgcggcagcg cccagggcat cgagggcagc atccgcagcc cccacctgat ggacatcctg 900
aacagcatca ccatctacac cgacgcccac cgcggcgagt actactggag cggccaccag 960
atcatggcca gccccgtcgg cttcagcggc cccgagttca ccttccccct gtacggcacg 1020
atgggcaacg ctgcacctca gcagcgcatc gtggcacagc tgggccaggg agtgtaccgc 1080
accctgagca gcaccctgta ccgtcgacct ttcaacatcg gcatcaacaa ccagcagctg 1140
agcgtgctgg acggcaccga gttcgcctac ggcaccagca gcaacctgcc cagcgccgtg 1200
taccgcaaga gcggcaccgt ggacagcctg gacgagatcc cccctcagaa caacaacgtg 1260
ccacctcgac agggcttcag ccaccgtctg agccacgtga gcatgttccg cagtggcttc 1320
agcaacagca gcgtgagcat catccgtgca cctatgttca gctggattca ccgcagtgcc 1380
gagttcaaca acatcatccc cagcagccag atcacccaga tccccctgac caagagcacc 1440
aacctgggca gcggcaccag cgtggtgaag ggccccggct tcaccggcgg cgacatcctg 1500
cgccgcacca gccccggcca gatcagcacc ctgcgcgtga acatcaccgc ccccctgagc 1560
cagcgctacc gcgtccgcat ccgctacgcc agcaccacca acctgcagtt ccacaccagc 1620
atcgacggcc gccccatcaa ccagggcaac ttcagcgcca ccatgagcag cggcagcaac 1680
ctgcagagcg gcagcttccg caccgtgggc ttcaccaccc ccttcaactt cagcaacggc 1740
agcagcgtgt tcaccctgag cgcccacgtg ttcaacagcg gcaacgaggt gtacatcgac 1800
cgcatcgagt tcgtgcccgc cgaggtgacc ttcgaggccg agtacgacct ggagagggct 1860
cagaaggccg tgaacgagct gttcaccagc agcaaccaga tcggcctgaa gaccgacgtg 1920
accgactacc acatcgacca ggtgagcaac ctggtggagt gcttaagcga cgagttctgc 1980
ctggacgaga agaaggagct gagcgagaag gtgaagcacg ccaagcgcct gagcgacgag 2040
cgcaacctgc tgcaggaccc caacttccgc ggcatcaacc gccagctgga ccgcggctgg 2100
cgaggcagca ccgatatcac catccagggc ggcgacgacg tgttcaagga gaactacgtg 2160
accctgctgg gcaccttcga cgagtgctac cccacctacc tgtaccagaa gatcgacgag 2220
agcaagctga aggcctacac ccgctaccag ctgcgcggct acatcgagga cagccaggac 2280
ctggaaatct acctgatccg ctacaacgcg aagcacgaga ccgtgaacgt gcccggcacc 2340
ggcagcctgt ggcccctgag cgcccccagc cccatcggca agtgcactag catccttttc 2400
attgataaga agagcaccgt tcactccaac tgcaaacttg tggctagtca ccacagccac 2460
cacttcagcc tggacatcga cgtgggctgc accgacctga acgaggacct gggcgtgtgg 2520
gtgatcttca agatcaagac ccaggacggc cacgcccgcc tgggcaatct agagttcctg 2580
gaggagaagc ccctggtggg cgaggccctg gcccgcgtga agcgtgctga gaagaagtgg 2640
cgcgacaagc gcgagaagct ggagtgggag accaacatcg tgtacaagga ggccaaggag 2700
agcgtggacg ccctgttcgt gaacagccag tacgaccgcc tgcaggccga caccaacatc 2760
gccatgatcc acgccgccga caagcgcgtg cacagcattc gcgaggccta cctgcccgag 2820
ctgagcgtga tccccggtgt gaacgccgcc atcttcgagg aactcgaggg ccgcatctag 2880
<210> 69
<211> 2880
<212> DNA
<213> 人工序列
<220>
<223> 突变型cry1Ab
<400> 69
atggacaaca accccaacat caacgagtgc atcccctaca actgcctgag caaccccgag 60
gtggaggtgc tgggcggcga gcgcatcgag accggctaca cccccatcga catcagcctg 120
agcctgaccc agttcctgct gagcgagttc gtgcccggcg ccggcttcgt gctgggcctg 180
gtggacatca tctggggcat cttcggcccc agccagtggg acgccttcct ggtgcagatc 240
gagcagttga taaaccaacg catagaggaa ttcgcccgca accaggccat cagccgcctg 300
gagggcctga gcaacctgta ccaaatctac gccgagagct tccgcgagtg ggaggccgac 360
cccaccaacc ccgccctgcg cgaggagatg cgcatccagt tcaacgacat gaacagcgcc 420
ctgaccaccg ccatccccct gttcgccgtg cagaactacc aggtgcccct gctgagcgtg 480
tacgtgcagg ccgccaacct gcacctgagc gtgctgcgcg acgtcagcgt gttcggccag 540
cgctggggct tcgacgccgc caccatcaac agccgctaca acgacctgac ccgcctgatc 600
ggcaactaca ccgaccacgc cgtgcgctgg tacaacaccg gcctggagcg cgtgtggggg 660
cccgacagcc gcgactggat caggtacaac cagttccgcc gcgagctgac cctgaccgtg 720
ctggacatcg tgagcctgtt ccccaactac gacagccgca cctaccccat ccgcaccgtg 780
agccagctga cccgcgagat ttacaccaac cccgtgctgg agaacttcga cggcagcttc 840
cgcggcagcg cccagggcat cgagggcagc atccgcagcc cccacctgat ggacatcctg 900
aacagcatca ccatctacac cgacgcccac cgcggcgagt actactggag cggccaccag 960
atcatggcca gccccgtcgg cttcagcggc cccgagttca ccttccccct gtacggcacg 1020
atgggcaacg ctgcacctca gcagcgcatc gtggcacagc tgggccaggg agtgtaccgc 1080
accctgagca gcaccctgta ccgtcgacct ttcaacatcg gcatcaacaa ccagcagctg 1140
agcgtgctgg acggcaccga gttcgcctac ggcaccagca gcaacctgcc cagcgccgtg 1200
taccgcaaga gcggcaccgt ggacagcctg gacgagatcc cccctcagaa caacaacgtg 1260
ccacctcgac agggcttcag ccaccgtctg agccacgtga gcatgttccg cagtggcttc 1320
agcaacagca gcgtgagcat catccgtgca cctatgttca gctggattca ccgcagtgcc 1380
gagttcaaca acatcatccc cagcagccag atcacccaga tccccctgac caagagcacc 1440
aacctgggca gcggcaccag cgtggtgaag ggccccggct tcaccggcgg cgacatcctg 1500
cgccgcacca gccccggcca gatcagcacc ctgcgcgtga acatcaccgc ccccctgagc 1560
cagcgctacc gcgtccgcat ccgctacgcc agcaccacca acctgcagtt ccacaccagc 1620
atcgacggcc gccccatcaa ccagggcaac ttcagcgcca ccatgagcag cggcagcaac 1680
ctgcagagcg gcagcttccg caccgtgggc ttcaccaccc ccttcaactt cagcaacggc 1740
agcagcgtgt tcaccctgag cgcccacgtg ttcaacagcg gcaacgaggt gtacatcgac 1800
cgcatcgagt tcgtgcccgc cgaggtgacc ttcgaggccg agtacgacct ggagagggct 1860
cagaaggccg tgaacgagct gttcaccagc agcaaccaga tcggcctgaa gaccgacgtg 1920
accgactacc acatcgacca ggtgagcaac ctggtggagt gcttaagcga cgagttctgc 1980
ctggacgaga agaaggagct gagcgagaag gtgaagcacg ccaagcgcct gagcgacgag 2040
cgcaacctgc tgcaggaccc caacttccgc ggcatcaacc gccagctgga ccgcggctgg 2100
cgaggcagca ccgatatcac catccagggc ggcgacgacg tgttcaagga gaactacgtg 2160
accctgctgg gcaccttcga cgagtgctac cccacctacc tgtaccagaa gatcgacgag 2220
agcaagctga aggcctacac ccgctaccag ctgcgcggct acatcgagga cagccaggac 2280
ctggaaatct acctgatccg ctacaacgcg aagcacgaga ccgtgaacgt gcccggcacc 2340
ggcagcctgt ggcccctgag cgcccccagc cccatcggca agtgcactag cacatccaac 2400
acgcacgaga tgacagatag agcagtgagc agaccatctc ttactagtca ccacagccac 2460
cacttcagcc tggacatcga cgtgggctgc accgacctga acgaggacct gggcgtgtgg 2520
gtgatcttca agatcaagac ccaggacggc cacgcccgcc tgggcaatct agagttcctg 2580
gaggagaagc ccctggtggg cgaggccctg gcccgcgtga agcgtgctga gaagaagtgg 2640
cgcgacaagc gcgagaagct ggagtgggag accaacatcg tgtacaagga ggccaaggag 2700
agcgtggacg ccctgttcgt gaacagccag tacgaccgcc tgcaggccga caccaacatc 2760
gccatgatcc acgccgccga caagcgcgtg cacagcattc gcgaggccta cctgcccgag 2820
ctgagcgtga tccccggtgt gaacgccgcc atcttcgagg aactcgaggg ccgcatctag 2880
<210> 70
<211> 2877
<212> DNA
<213> 人工序列
<220>
<223> 突变型cry1Ab
<400> 70
atggacaaca accccaacat caacgagtgc atcccctaca actgcctgag caaccccgag 60
gtggaggtgc tgggcggcga gcgcatcgag accggctaca cccccatcga catcagcctg 120
agcctgaccc agttcctgct gagcgagttc gtgcccggcg ccggcttcgt gctgggcctg 180
gtggacatca tctggggcat cttcggcccc agccagtggg acgccttcct ggtgcagatc 240
gagcagttga taaaccaacg catagaggaa ttcgcccgca accaggccat cagccgcctg 300
gagggcctga gcaacctgta ccaaatctac gccgagagct tccgcgagtg ggaggccgac 360
cccaccaacc ccgccctgcg cgaggagatg cgcatccagt tcaacgacat gaacagcgcc 420
ctgaccaccg ccatccccct gttcgccgtg cagaactacc aggtgcccct gctgagcgtg 480
tacgtgcagg ccgccaacct gcacctgagc gtgctgcgcg acgtcagcgt gttcggccag 540
cgctggggct tcgacgccgc caccatcaac agccgctaca acgacctgac ccgcctgatc 600
ggcaactaca ccgaccacgc cgtgcgctgg tacaacaccg gcctggagcg cgtgtggggg 660
cccgacagcc gcgactggat caggtacaac cagttccgcc gcgagctgac cctgaccgtg 720
ctggacatcg tgagcctgtt ccccaactac gacagccgca cctaccccat ccgcaccgtg 780
agccagctga cccgcgagat ttacaccaac cccgtgctgg agaacttcga cggcagcttc 840
cgcggcagcg cccagggcat cgagggcagc atccgcagcc cccacctgat ggacatcctg 900
aacagcatca ccatctacac cgacgcccac cgcggcgagt actactggag cggccaccag 960
atcatggcca gccccgtcgg cttcagcggc cccgagttca ccttccccct gtacggcacg 1020
atgggcaacg ctgcacctca gcagcgcatc gtggcacagc tgggccaggg agtgtaccgc 1080
accctgagca gcaccctgta ccgtcgacct ttcaacatcg gcatcaacaa ccagcagctg 1140
agcgtgctgg acggcaccga gttcgcctac ggcaccagca gcaacctgcc cagcgccgtg 1200
taccgcaaga gcggcaccgt ggacagcctg gacgagatcc cccctcagaa caacaacgtg 1260
ccacctcgac agggcttcag ccaccgtctg agccacgtga gcatgttccg cagtggcttc 1320
agcaacagca gcgtgagcat catccgtgca cctatgttca gctggattca ccgcagtgcc 1380
gagttcaaca acatcatccc cagcagccag atcacccaga tccccctgac caagagcacc 1440
aacctgggca gcggcaccag cgtggtgaag ggccccggct tcaccggcgg cgacatcctg 1500
cgccgcacca gccccggcca gatcagcacc ctgcgcgtga acatcaccgc ccccctgagc 1560
cagcgctacc gcgtccgcat ccgctacgcc agcaccacca acctgcagtt ccacaccagc 1620
atcgacggcc gccccatcaa ccagggcaac ttcagcgcca ccatgagcag cggcagcaac 1680
ctgcagagcg gcagcttccg caccgtgggc ttcaccaccc ccttcaactt cagcaacggc 1740
agcagcgtgt tcaccctgag cgcccacgtg ttcaacagcg gcaacgaggt gtacatcgac 1800
cgcatcgagt tcgtgcccgc cgaggtgacc ttcgaggccg agtacgacct ggagagggct 1860
cagaaggccg tgaacgagct gttcaccagc agcaaccaga tcggcctgaa gaccgacgtg 1920
accgactacc acatcgacca ggtgagcaac ctggtggagt gcttaagcga cgagttctgc 1980
ctggacgaga agaaggagct gagcgagaag gtgaagcacg ccaagcgcct gagcgacgag 2040
cgcaacctgc tgcaggaccc caacttccgc ggcatcaacc gccagctgga ccgcggctgg 2100
cgaggcagca ccgatatcac catccagggc ggcgacgacg tgttcaagga gaactacgtg 2160
accctgctgg gcaccttcga cgagtgctac cccacctacc tgtaccagaa gatcgacgag 2220
agcaagctga aggcctacac ccgctaccag ctgcgcggct acatcgagga cagccaggac 2280
ctggaaatct acctgatccg ctacaacgcg aagcacgaga ccgtgaacgt gcccggcacc 2340
ggcagcctgt ggcccctgag cgcccccagc cccatcggca agtgcactag catctctgtt 2400
gcctttttga ttgaaccgtg ccaacagctt gttggattta ctagtcacca cagccaccac 2460
ttcagcctgg acatcgacgt gggctgcacc gacctgaacg aggacctggg cgtgtgggtg 2520
atcttcaaga tcaagaccca ggacggccac gcccgcctgg gcaatctaga gttcctggag 2580
gagaagcccc tggtgggcga ggccctggcc cgcgtgaagc gtgctgagaa gaagtggcgc 2640
gacaagcgcg agaagctgga gtgggagacc aacatcgtgt acaaggaggc caaggagagc 2700
gtggacgccc tgttcgtgaa cagccagtac gaccgcctgc aggccgacac caacatcgcc 2760
atgatccacg ccgccgacaa gcgcgtgcac agcattcgcg aggcctacct gcccgagctg 2820
agcgtgatcc ccggtgtgaa cgccgccatc ttcgaggaac tcgagggccg catctag 2877
<210> 71
<211> 2880
<212> DNA
<213> 人工序列
<220>
<223> 突变型cry1Ab
<400> 71
atggacaaca accccaacat caacgagtgc atcccctaca actgcctgag caaccccgag 60
gtggaggtgc tgggcggcga gcgcatcgag accggctaca cccccatcga catcagcctg 120
agcctgaccc agttcctgct gagcgagttc gtgcccggcg ccggcttcgt gctgggcctg 180
gtggacatca tctggggcat cttcggcccc agccagtggg acgccttcct ggtgcagatc 240
gagcagttga taaaccaacg catagaggaa ttcgcccgca accaggccat cagccgcctg 300
gagggcctga gcaacctgta ccaaatctac gccgagagct tccgcgagtg ggaggccgac 360
cccaccaacc ccgccctgcg cgaggagatg cgcatccagt tcaacgacat gaacagcgcc 420
ctgaccaccg ccatccccct gttcgccgtg cagaactacc aggtgcccct gctgagcgtg 480
tacgtgcagg ccgccaacct gcacctgagc gtgctgcgcg acgtcagcgt gttcggccag 540
cgctggggct tcgacgccgc caccatcaac agccgctaca acgacctgac ccgcctgatc 600
ggcaactaca ccgaccacgc cgtgcgctgg tacaacaccg gcctggagcg cgtgtggggg 660
cccgacagcc gcgactggat caggtacaac cagttccgcc gcgagctgac cctgaccgtg 720
ctggacatcg tgagcctgtt ccccaactac gacagccgca cctaccccat ccgcaccgtg 780
agccagctga cccgcgagat ttacaccaac cccgtgctgg agaacttcga cggcagcttc 840
cgcggcagcg cccagggcat cgagggcagc atccgcagcc cccacctgat ggacatcctg 900
aacagcatca ccatctacac cgacgcccac cgcggcgagt actactggag cggccaccag 960
atcatggcca gccccgtcgg cttcagcggc cccgagttca ccttccccct gtacggcacg 1020
atgggcaacg ctgcacctca gcagcgcatc gtggcacagc tgggccaggg agtgtaccgc 1080
accctgagca gcaccctgta ccgtcgacct ttcaacatcg gcatcaacaa ccagcagctg 1140
agcgtgctgg acggcaccga gttcgcctac ggcaccagca gcaacctgcc cagcgccgtg 1200
taccgcaaga gcggcaccgt ggacagcctg gacgagatcc cccctcagaa caacaacgtg 1260
ccacctcgac agggcttcag ccaccgtctg agccacgtga gcatgttccg cagtggcttc 1320
agcaacagca gcgtgagcat catccgtgca cctatgttca gctggattca ccgcagtgcc 1380
gagttcaaca acatcatccc cagcagccag atcacccaga tccccctgac caagagcacc 1440
aacctgggca gcggcaccag cgtggtgaag ggccccggct tcaccggcgg cgacatcctg 1500
cgccgcacca gccccggcca gatcagcacc ctgcgcgtga acatcaccgc ccccctgagc 1560
cagcgctacc gcgtccgcat ccgctacgcc agcaccacca acctgcagtt ccacaccagc 1620
atcgacggcc gccccatcaa ccagggcaac ttcagcgcca ccatgagcag cggcagcaac 1680
ctgcagagcg gcagcttccg caccgtgggc ttcaccaccc ccttcaactt cagcaacggc 1740
agcagcgtgt tcaccctgag cgcccacgtg ttcaacagcg gcaacgaggt gtacatcgac 1800
cgcatcgagt tcgtgcccgc cgaggtgacc ttcgaggccg agtacgacct ggagagggct 1860
cagaaggccg tgaacgagct gttcaccagc agcaaccaga tcggcctgaa gaccgacgtg 1920
accgactacc acatcgacca ggtgagcaac ctggtggagt gcttaagcga cgagttctgc 1980
ctggacgaga agaaggagct gagcgagaag gtgaagcacg ccaagcgcct gagcgacgag 2040
cgcaacctgc tgcaggaccc caacttccgc ggcatcaacc gccagctgga ccgcggctgg 2100
cgaggcagca ccgatatcac catccagggc ggcgacgacg tgttcaagga gaactacgtg 2160
accctgctgg gcaccttcga cgagtgctac cccacctacc tgtaccagaa gatcgacgag 2220
agcaagctga aggcctacac ccgctaccag ctgcgcggct acatcgagga cagccaggac 2280
ctggaaatct acctgatccg ctacaacgcg aagcacgaga ccgtgaacgt gcccggcacc 2340
ggcagcctgt ggcccctgag cgcccccagc cccatcggca agtgcactag cgacgacccc 2400
aaaggacagg tttcccacag ctttattgaa caattgtttg catctagtca ccacagccac 2460
cacttcagcc tggacatcga cgtgggctgc accgacctga acgaggacct gggcgtgtgg 2520
gtgatcttca agatcaagac ccaggacggc cacgcccgcc tgggcaatct agagttcctg 2580
gaggagaagc ccctggtggg cgaggccctg gcccgcgtga agcgtgctga gaagaagtgg 2640
cgcgacaagc gcgagaagct ggagtgggag accaacatcg tgtacaagga ggccaaggag 2700
agcgtggacg ccctgttcgt gaacagccag tacgaccgcc tgcaggccga caccaacatc 2760
gccatgatcc acgccgccga caagcgcgtg cacagcattc gcgaggccta cctgcccgag 2820
ctgagcgtga tccccggtgt gaacgccgcc atcttcgagg aactcgaggg ccgcatctag 2880
<210> 72
<211> 2877
<212> DNA
<213> 人工序列
<220>
<223> 突变型cry1Ab
<400> 72
atggacaaca accccaacat caacgagtgc atcccctaca actgcctgag caaccccgag 60
gtggaggtgc tgggcggcga gcgcatcgag accggctaca cccccatcga catcagcctg 120
agcctgaccc agttcctgct gagcgagttc gtgcccggcg ccggcttcgt gctgggcctg 180
gtggacatca tctggggcat cttcggcccc agccagtggg acgccttcct ggtgcagatc 240
gagcagttga taaaccaacg catagaggaa ttcgcccgca accaggccat cagccgcctg 300
gagggcctga gcaacctgta ccaaatctac gccgagagct tccgcgagtg ggaggccgac 360
cccaccaacc ccgccctgcg cgaggagatg cgcatccagt tcaacgacat gaacagcgcc 420
ctgaccaccg ccatccccct gttcgccgtg cagaactacc aggtgcccct gctgagcgtg 480
tacgtgcagg ccgccaacct gcacctgagc gtgctgcgcg acgtcagcgt gttcggccag 540
cgctggggct tcgacgccgc caccatcaac agccgctaca acgacctgac ccgcctgatc 600
ggcaactaca ccgaccacgc cgtgcgctgg tacaacaccg gcctggagcg cgtgtggggg 660
cccgacagcc gcgactggat caggtacaac cagttccgcc gcgagctgac cctgaccgtg 720
ctggacatcg tgagcctgtt ccccaactac gacagccgca cctaccccat ccgcaccgtg 780
agccagctga cccgcgagat ttacaccaac cccgtgctgg agaacttcga cggcagcttc 840
cgcggcagcg cccagggcat cgagggcagc atccgcagcc cccacctgat ggacatcctg 900
aacagcatca ccatctacac cgacgcccac cgcggcgagt actactggag cggccaccag 960
atcatggcca gccccgtcgg cttcagcggc cccgagttca ccttccccct gtacggcacg 1020
atgggcaacg ctgcacctca gcagcgcatc gtggcacagc tgggccaggg agtgtaccgc 1080
accctgagca gcaccctgta ccgtcgacct ttcaacatcg gcatcaacaa ccagcagctg 1140
agcgtgctgg acggcaccga gttcgcctac ggcaccagca gcaacctgcc cagcgccgtg 1200
taccgcaaga gcggcaccgt ggacagcctg gacgagatcc cccctcagaa caacaacgtg 1260
ccacctcgac agggcttcag ccaccgtctg agccacgtga gcatgttccg cagtggcttc 1320
agcaacagca gcgtgagcat catccgtgca cctatgttca gctggattca ccgcagtgcc 1380
gagttcaaca acatcatccc cagcagccag atcacccaga tccccctgac caagagcacc 1440
aacctgggca gcggcaccag cgtggtgaag ggccccggct tcaccggcgg cgacatcctg 1500
cgccgcacca gccccggcca gatcagcacc ctgcgcgtga acatcaccgc ccccctgagc 1560
cagcgctacc gcgtccgcat ccgctacgcc agcaccacca acctgcagtt ccacaccagc 1620
atcgacggcc gccccatcaa ccagggcaac ttcagcgcca ccatgagcag cggcagcaac 1680
ctgcagagcg gcagcttccg caccgtgggc ttcaccaccc ccttcaactt cagcaacggc 1740
agcagcgtgt tcaccctgag cgcccacgtg ttcaacagcg gcaacgaggt gtacatcgac 1800
cgcatcgagt tcgtgcccgc cgaggtgacc ttcgaggccg agtacgacct ggagagggct 1860
cagaaggccg tgaacgagct gttcaccagc agcaaccaga tcggcctgaa gaccgacgtg 1920
accgactacc acatcgacca ggtgagcaac ctggtggagt gcttaagcga cgagttctgc 1980
ctggacgaga agaaggagct gagcgagaag gtgaagcacg ccaagcgcct gagcgacgag 2040
cgcaacctgc tgcaggaccc caacttccgc ggcatcaacc gccagctgga ccgcggctgg 2100
cgaggcagca ccgatatcac catccagggc ggcgacgacg tgttcaagga gaactacgtg 2160
accctgctgg gcaccttcga cgagtgctac cccacctacc tgtaccagaa gatcgacgag 2220
agcaagctga aggcctacac ccgctaccag ctgcgcggct acatcgagga cagccaggac 2280
ctggaaatct acctgatccg ctacaacgcg aagcacgaga ccgtgaacgt gcccggcacc 2340
ggcagcctgt ggcccctgag cgcccccagc cccatcggca agtgcactag tcctttctct 2400
ctctttcttt ctctctgctt tattgaactt ctcaaagtta ctagtcacca cagccaccac 2460
ttcagcctgg acatcgacgt gggctgcacc gacctgaacg aggacctggg cgtgtgggtg 2520
atcttcaaga tcaagaccca ggacggccac gcccgcctgg gcaatctaga gttcctggag 2580
gagaagcccc tggtgggcga ggccctggcc cgcgtgaagc gtgctgagaa gaagtggcgc 2640
gacaagcgcg agaagctgga gtgggagacc aacatcgtgt acaaggaggc caaggagagc 2700
gtggacgccc tgttcgtgaa cagccagtac gaccgcctgc aggccgacac caacatcgcc 2760
atgatccacg ccgccgacaa gcgcgtgcac agcattcgcg aggcctacct gcccgagctg 2820
agcgtgatcc ccggtgtgaa cgccgccatc ttcgaggaac tcgagggccg catctag 2877
<210> 73
<211> 2823
<212> DNA
<213> 人工序列
<220>
<223> 突变型cry1Ab
<400> 73
atggacaaca accccaacat caacgagtgc atcccctaca actgcctgag caaccccgag 60
gtggaggtgc tgggcggcga gcgcatcgag accggctaca cccccatcga catcagcctg 120
agcctgaccc agttcctgct gagcgagttc gtgcccggcg ccggcttcgt gctgggcctg 180
gtggacatca tctggggcat cttcggcccc agccagtggg acgccttcct ggtgcagatc 240
gagcagttga taaaccaacg catagaggaa ttcgcccgca accaggccat cagccgcctg 300
gagggcctga gcaacctgta ccaaatctac gccgagagct tccgcgagtg ggaggccgac 360
cccaccaacc ccgccctgcg cgaggagatg cgcatccagt tcaacgacat gaacagcgcc 420
ctgaccaccg ccatccccct gttcgccgtg cagaactacc aggtgcccct gctgagcgtg 480
tacgtgcagg ccgccaacct gcacctgagc gtgctgcgcg acgtcagcgt gttcggccag 540
cgctggggct tcgacgccgc caccatcaac agccgctaca acgacctgac ccgcctgatc 600
ggcaactaca ccgaccacgc cgtgcgctgg tacaacaccg gcctggagcg cgtgtggggg 660
cccgacagcc gcgactggat caggtacaac cagttccgcc gcgagctgac cctgaccgtg 720
ctggacatcg tgagcctgtt ccccaactac gacagccgca cctaccccat ccgcaccgtg 780
agccagctga cccgcgagat ttacaccaac cccgtgctgg agaacttcga cggcagcttc 840
cgcggcagcg cccagggcat cgagggcagc atccgcagcc cccacctgat ggacatcctg 900
aacagcatca ccatctacac cgacgcccac cgcggcgagt actactggag cggccaccag 960
atcatggcca gccccgtcgg cttcagcggc cccgagttca ccttccccct gtacggcacg 1020
atgggcaacg ctgcacctca gcagcgcatc gtggcacagc tgggccaggg agtgtaccgc 1080
accctgagca gcaccctgta ccgtcgacct ttcaacatcg gcatcaacaa ccagcagctg 1140
agcgtgctgg acggcaccga gttcgcctac ggcaccagca gcaacctgcc cagcgccgtg 1200
taccgcaaga gcggcaccgt ggacagcctg gacgagatcc cccctcagaa caacaacgtg 1260
ccacctcgac agggcttcag ccaccgtctg agccacgtga gcatgttccg cagtggcttc 1320
agcaacagca gcgtgagcat catccgtgca cctatgttca gctggattca ccgcagtgcc 1380
gagttcaaca acatcatccc cagcagccag atcacccaga tccccctgac caagagcacc 1440
aacctgggca gcggcaccag cgtggtgaag ggccccggct tcaccggcgg cgacatcctg 1500
cgccgcacca gccccggcca gatcagcacc ctgcgcgtga acatcaccgc ccccctgagc 1560
cagcgctacc gcgtccgcat ccgctacgcc agcaccacca acctgcagtt ccacaccagc 1620
atcgacggcc gccccatcaa ccagggcaac ttcagcgcca ccatgagcag cggcagcaac 1680
ctgcagagcg gcagcttccg caccgtgggc ttcaccaccc ccttcaactt cagcaacggc 1740
agcagcgtgt tcaccctgag cgcccacgtg ttcaacagcg gcaacgaggt gtacatcgac 1800
cgcatcgagt tcgtgcccgc cgaggtgacc ttcgaggccg agtacgacct ggagagggct 1860
cagaaggccg tgaacgagct gttcaccagc agcaaccaga tcggcctgaa gaccgacgtg 1920
accgactacc acatcgacca ggtgagcaac ctggtggagt gcttaagcga cgagttctgc 1980
ctggacgaga agaaggagct gagcgagaag gtgaagcacg ccaagcgcct gagcgacgag 2040
cgcaacctgc tgcaggaccc caacttccgc ggcatcaacc gccagctgga ccgcggctgg 2100
cgaggcagca ccgatatcac catccagggc ggcgacgacg tgttcaagga gaactacgtg 2160
accctgctgg gcaccttcga cgagtgctac cccacctacc tgtaccagaa gatcgacgag 2220
agcaagctga aggcctacac ccgctaccag ctgcgcggct acatcgagga cagccaggac 2280
ctggaaatct acctgatccg ctacaacgcg aagcacgaga ccgtgaacgt gcccggcacc 2340
ggcagcctgt ggcccctgag cgcccccagc cccatcggca agtgcactag tcaccacagc 2400
caccacttca gcctggacat cgacgtgggc tgcaccgacc tgaacgagga cctgggcgtg 2460
tgggtgatct tcaagatcaa gacccaggac ggccacgccc gcctgggcaa tctagagttc 2520
ctggaggaga agcccctggt gggcgaggcc ctggcccgcg tgaagcgtgc tgagaagaag 2580
tggcgcgaca agcgcgagaa gctggagtgg gagaccaaca tcgtgtacaa ggaggccaag 2640
gagagcgtgg acgccctgtt cgtgaacagc cagtacgacc gcctgcaggc cgacaccaac 2700
atcgccatga tccacgccgc cgacaagcgc gtgcacagca ttcgcgaggc ctacctgccc 2760
gagctgagcg tgatccccgg tgtgaacgcc gccatcttcg aggaactcga gggccgcatc 2820
tag 2823
<210> 74
<211> 52
<212> RNA
<213> 人工序列
<220>
<223> 23708靶插入序列
<400> 74
ugaugcacag auugggauga gauauuggag gaaaacaaag agggacugac aa 52
<210> 75
<211> 51
<212> RNA
<213> 人工序列
<220>
<223> 23711靶插入序列
<400> 75
ugucccucac uacguccggu ucaguugccu cuaauccuau cccucgccac g 51
<210> 76
<211> 52
<212> RNA
<213> 人工序列
<220>
<223> 23712靶插入序列
<400> 76
uauacgcaca cacauguuga gauauuagag gaaacugaag aauuacgcca ca 52
<210> 77
<211> 52
<212> RNA
<213> 人工序列
<220>
<223> 23715靶插入序列
<400> 77
cgccaaauga aucuucuuga gauuuuagag uaaacugaaa cugacuguug ca 52
<210> 78
<211> 54
<212> RNA
<213> 人工序列
<220>
<223> 23716靶插入序列
<400> 78
uauacgcaca cacauguuga gauauuagag gaaacugaag aauuacgcca cauu 54
<210> 79
<211> 52
<212> DNA
<213> 人工序列
<220>
<223> 23708靶插入序列
<400> 79
tgatgcacag attgggatga gatattggag gaaaacaaag agggactgac aa 52
<210> 80
<211> 51
<212> DNA
<213> 人工序列
<220>
<223> 23711靶插入序列
<400> 80
tgtccctcac tacgtccggt tcagttgcct ctaatcctat ccctcgccac g 51
<210> 81
<211> 52
<212> DNA
<213> 人工序列
<220>
<223> 23712靶插入序列
<400> 81
tatacgcaca cacatgttga gatattagag gaaactgaag aattacgcca ca 52
<210> 82
<211> 52
<212> DNA
<213> 人工序列
<220>
<223> 23715靶插入序列
<400> 82
cgccaaatga atcttcttga gattttagag taaactgaaa ctgactgttg ca 52
<210> 83
<211> 54
<212> DNA
<213> 人工序列
<220>
<223> 23716靶插入序列
<400> 83
tatacgcaca cacatgttga gatattagag gaaactgaag aattacgcca catt 54
<210> 84
<211> 4579
<212> DNA
<213> 人工序列
<220>
<223> 23705 vip3表达盒
<400> 84
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 60
agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 120
tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 180
tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 240
gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 300
ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 360
gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 420
agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 480
taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 540
aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 600
cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 660
cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 720
acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 780
ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 840
gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 900
ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 960
cccgtcggca cctccgcttc ctaggtacgc cgctcgtcct cccccccccc ccctctctac 1020
cttctctaga tcggcgttcc ggtccatagt tagggcccgg tagttctact tctgttcatg 1080
tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga 1140
cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg 1200
ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca 1260
tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt 1320
catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt 1380
ctagatcgga gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt 1440
atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc 1500
taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt 1560
tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt 1620
agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca 1680
tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca 1740
tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg 1800
ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tattttgatc 1860
ttgatatact tggatgatgg catatccagc agctatatgt ggattttttt agccctgcct 1920
tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg 1980
tgttacttct gcaggatcct ctaagtagca gccatgaaca agaacaacac caagctgagc 2040
acccgcgccc tgccgagctt catcgactac ttcaacggca tctacggctt cgccaccggc 2100
atcaaggaca tcatgaacat gatcttcaag accgacaccg gcggcgacct gaccctggac 2160
gagatcctga agaaccagca gctgctgaac gacatcagcg gcaagctgga cggcgtgaac 2220
ggcagcctga acgacctgat cgcccagggc aacctgaaca ccgagctgag caaggagatc 2280
cttaagatcg ccaacgagca gaaccaggtg ctgaacgacg tgaacaacaa gctggacgcc 2340
atcaacacca tgctgcgcgt gtacctgccg aagatcacca gcatgctgag cgacgtgatt 2400
aagcagaact acgccctgag cctgcagatc gagtacctga gcaagcagct gcaggagatc 2460
agcgacaagc tggacatcat caacgtgaac gtcctgatca acagcaccct gaccgagatc 2520
accccggcct accagcgcat caagtacgtg aacgagaagt tcgaagagct gaccttcgcc 2580
accgagacca gcagcaaggt gaagaaggac ggcagcccgg ccgacatcct ggacgagctg 2640
accgagctga ccgagctggc gaagagcgtg accaagaacg acgtggacgg cttcgagttc 2700
tacctgaaca ccttccacga cgtgatggtg ggcaacaacc tgttcggccg cagcgccctg 2760
aagaccgcca gcgagctgat caccaaggag aacgtgaaga ccagcggcag cgaggtgggc 2820
aacgtgtaca acttcctgat cgtgctgacc gccctgcagg cccaggcctt cctgaccctg 2880
accacctgtc gcaagctgct gggcctggcc gacatcgact acaccagcat catgaacgag 2940
cacttgaaca aggagaagga ggagttccgc gtgaacatcc tgccgaccct gagcaacacc 3000
ttcagcaacc cgaactacgc caaggtgaag ggcagcgacg aggacgccaa gatgatcgtg 3060
gaggctaagc cgggccacgc gttgatcggc ttcgagatca gcaacgacag catcaccgtg 3120
ctgaaggtgt acgaggccaa gctgaagcag aactaccagg tggacaagga cagcttgagc 3180
gaggtgatct acggcgacat ggacaagctg ctgtgtccgg accagagcga gcaaatctac 3240
tacaccaaca acatcgtgtt cccgaacgag tacgtgatca ccaagatcga cttcaccaag 3300
aagatgaaga ccctgcgcta cgaggtgacc gccaacttct acgacagcag caccggcgag 3360
atcgacctga acaagaagaa ggtggagagc agcgaggccg agtaccgcac cctgagcgcg 3420
aacgacgacg gcgtctacat gccactgggc gtgatcagcg agaccttcct gaccccgatc 3480
aacggctttg gcctgcaggc cgacgagaac agccgcctga tcaccctgac ctgtaagagc 3540
tacctgcgcg agctgctgct agccaccgac ctgagcaaca aggagaccaa gctgatcgtg 3600
ccaccgagcg gcttcatcag caacatcgtg gagaacggca gcatcgagga ggacaacctg 3660
gagccgtgga aggccaacaa caagaacgcc tacgtcgacc acaccggcgg cgtgaacggc 3720
accaaggccc tgtacgtgca caaggacggc ggcatcagcc agttcatcgg cgacaagctg 3780
aagccgaaga ccgagtacgt gatccagtac accgtgaagg gcaagccatc gattcacctg 3840
aaggacgaga acaccggcta catccactac gaggacacca acaacaacct ggaggactac 3900
cagaccatca acaagcgctt caccaccggc accgacctga agggcgtgta cctgatcctg 3960
aagagccaga acggcgacga ggcctggggc gacaacttca tcatcctgga gatcagcccg 4020
agcgagaagc tgctgagccc ggagctgatc aacaccaaca actggaccag caccggcagc 4080
accaacatca gcggcaacac cctgaccctg taccagggcg gccgcggcat cctgaagcag 4140
aacctgcagc tggacagctt cagcacctac cgcgtgtact tcagcgtgag cggcgacgcc 4200
aacgtgcgca tccgcaactc ccgcgaggtg ctgttcgaga agaggtacat gagcggcgcc 4260
aaggacgtga gcgagatgtt caccaccaag ttcgagaagg acaacttcta catcgagctg 4320
agccagggca acaacctgta cggcggcccg atcgtgcact tctacgacgt gagcatcaag 4380
taggagctct agatcttatc tgcacaaagt ggagtagtca gtcatcgatc aggaaccaga 4440
caccagactt ttattcatac agtgaagtga agtgaagtgc agtgcagtga gttgctggtt 4500
tttgtacaac ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat 4560
tcctaaaacc aaaatccag 4579
<210> 85
<211> 4625
<212> DNA
<213> 人工序列
<220>
<223> 23708 vip3表达盒
<400> 85
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 60
agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 120
tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 180
tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 240
gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 300
ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 360
gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 420
agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 480
taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 540
aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 600
cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 660
cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 720
acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 780
ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 840
gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 900
ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 960
cccgtcggca cctccgcttc ctaggtacgc cgctcgtcct cccccccccc ccctctctac 1020
cttctctaga tcggcgttcc ggtccatagt tagggcccgg tagttctact tctgttcatg 1080
tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga 1140
cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg 1200
ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca 1260
tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt 1320
catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt 1380
ctagatcgga gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt 1440
atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc 1500
taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt 1560
tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt 1620
agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca 1680
tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca 1740
tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg 1800
ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tattttgatc 1860
ttgatatact tggatgatgg catatccagc agctatatgt ggattttttt agccctgcct 1920
tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg 1980
tgttacttct gcaggatcct ctaagtagca gccatgaaca agaacaacac caagctgagc 2040
acccgcgccc tgccgagctt catcgactac ttcaacggca tctacggctt cgccaccggc 2100
atcaaggaca tcatgaacat gatcttcaag accgacaccg gcggcgacct gaccctggac 2160
gagatcctga agaaccagca gctgctgaac gacatcagcg gcaagctgga cggcgtgaac 2220
ggcagcctga acgacctgat cgcccagggc aacctgaaca ccgagctgag caaggagatc 2280
cttaagatcg ccaacgagca gaaccaggtg ctgaacgacg tgaacaacaa gctggacgcc 2340
atcaacacca tgctgcgcgt gtacctgccg aagatcacca gcatgctgag cgacgtgatt 2400
aagcagaact acgccctgag cctgcagatc gagtacctga gcaagcagct gcaggagatc 2460
agcgacaagc tggacatcat caacgtgaac gtcctgatca acagcaccct gaccgagatc 2520
accccggcct accagcgcat caagtacgtg aacgagaagt tcgaagagct gaccttcgcc 2580
accgagacca gcagcaaggt gaagaaggac ggcagcccgg ccgacatcct ggacgagctg 2640
accgagctga ccgagctggc gaagagcgtg accaagaacg acgtggacgg cttcgagttc 2700
tacctgaaca ccttccacga cgtgatggtg ggcaacaacc tgttcggccg cagcgccctg 2760
aagaccgcca gcgagctgat caccaaggag aacgtgaaga ccagcggcag cgaggtgggc 2820
aacgtgtaca acttcctgat cgtgctgacc gccctgcagg cccaggcctt cctgaccctg 2880
accacctgtc gcaagctgct gggcctggcc gacatcgact acaccagcat catgaacgag 2940
cacttgaaca aggagaagga ggagttccgc gtgaacatcc tgccgaccct gagcaacacc 3000
ttcagcaacc cgaactacgc caaggtgaag ggcagcgacg aggacgccaa gatgatcgtg 3060
gaggctaagc cgggccacgc gttgatcggc ttcgagatca gcaacgacag catcaccgtg 3120
ctgaaggtgt acgaggccaa gctgaagcag aactaccagg tggacaagga cagcttgagc 3180
gaggtgatct acggcgacat ggacaagctg ctgtgtccgg accagagcga gcaaatctac 3240
tacaccaaca acatcgtgtt cccgaacgag tacgtgatca ccaagatcga cttcaccaag 3300
aagatgaaga ccctgcgcta cgaggtgacc gccaacttct acgacagcag caccggcgag 3360
atcgacctga acaagaagaa ggtggagagc agcgaggccg agtaccgcac cctgagcgcg 3420
aacgacgacg gcgtctacat gccactgggc gtgatcagcg agaccttcct gaccccgatc 3480
aacggctttg gcctgcaggc cgacgagaac agccgcctga tcaccctgac ctgtaagagc 3540
tacctgcgcg agctgctgct agccaccgac ctgagcaaca aggagaccaa gctgatcgtg 3600
ccaccgagcg gcttcatcag caacatcgtg gagaacggca gcatcgagga ggacaacctg 3660
gagccgtgga aggccaacaa caagaacgcc tacgtcgacc acaccggcgg cgtgaacggc 3720
accaaggccc tgtacgtgca caaggacggc ggcatcagcc agttcatcgg cgacaagctg 3780
aagccgaaga ccgagtacgt gatccagtac accgtgaagg gcaagccatc gattcacctg 3840
aaggacgaga acaccggcta catccactac gaggacacca acaacaacct ggaggactac 3900
cagaccatca acaagcgctt caccaccggc accgacctga agggcgtgta cctgatcctg 3960
aagagccaga acggcgacga ggcctggggc gacaacttca tcatcctgga gatcagcccg 4020
agcgagaagc tgctgagccc ggagctgatc aacaccaaca actggaccag caccggcagc 4080
accaacatca gcggcaacac cctgaccctg taccagggcg gccgcggcat cctgaagcag 4140
aacctgcagc tggacagctt cagcacctac cgcgtgtact tcagcgtgag cggcgacgcc 4200
aacgtgcgca tccgcaactc ccgcgaggtg ctgttcgaga agaggtacat gagcggcgcc 4260
aaggacgtga gcgagatgtt caccaccaag ttcgagaagg acaacttcta catcgagctg 4320
agccagggca acaacctgta cggcggcccg atcgtgcact tctacgacgt gagcatcaag 4380
taggagctct gatgcacaga ttgggatgag atattggagg aaaacaaaga gggactgaca 4440
attatctgca caaagtggag tagtcagtca tcgatcagga accagacacc agacttttat 4500
tcatacagtg aagtgaagtg aagtgcagtg cagtgagttg ctggtttttg tacaacttag 4560
tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa 4620
tccag 4625
<210> 86
<211> 4624
<212> DNA
<213> 人工序列
<220>
<223> 23711 vip3表达盒
<400> 86
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 60
agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 120
tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 180
tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 240
gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 300
ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 360
gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 420
agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 480
taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 540
aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 600
cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 660
cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 720
acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 780
ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 840
gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 900
ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 960
cccgtcggca cctccgcttc ctaggtacgc cgctcgtcct cccccccccc ccctctctac 1020
cttctctaga tcggcgttcc ggtccatagt tagggcccgg tagttctact tctgttcatg 1080
tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga 1140
cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg 1200
ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca 1260
tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt 1320
catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt 1380
ctagatcgga gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt 1440
atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc 1500
taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt 1560
tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt 1620
agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca 1680
tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca 1740
tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg 1800
ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tattttgatc 1860
ttgatatact tggatgatgg catatccagc agctatatgt ggattttttt agccctgcct 1920
tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg 1980
tgttacttct gcaggatcct ctaagtagca gccatgaaca agaacaacac caagctgagc 2040
acccgcgccc tgccgagctt catcgactac ttcaacggca tctacggctt cgccaccggc 2100
atcaaggaca tcatgaacat gatcttcaag accgacaccg gcggcgacct gaccctggac 2160
gagatcctga agaaccagca gctgctgaac gacatcagcg gcaagctgga cggcgtgaac 2220
ggcagcctga acgacctgat cgcccagggc aacctgaaca ccgagctgag caaggagatc 2280
cttaagatcg ccaacgagca gaaccaggtg ctgaacgacg tgaacaacaa gctggacgcc 2340
atcaacacca tgctgcgcgt gtacctgccg aagatcacca gcatgctgag cgacgtgatt 2400
aagcagaact acgccctgag cctgcagatc gagtacctga gcaagcagct gcaggagatc 2460
agcgacaagc tggacatcat caacgtgaac gtcctgatca acagcaccct gaccgagatc 2520
accccggcct accagcgcat caagtacgtg aacgagaagt tcgaagagct gaccttcgcc 2580
accgagacca gcagcaaggt gaagaaggac ggcagcccgg ccgacatcct ggacgagctg 2640
accgagctga ccgagctggc gaagagcgtg accaagaacg acgtggacgg cttcgagttc 2700
tacctgaaca ccttccacga cgtgatggtg ggcaacaacc tgttcggccg cagcgccctg 2760
aagaccgcca gcgagctgat caccaaggag aacgtgaaga ccagcggcag cgaggtgggc 2820
aacgtgtaca acttcctgat cgtgctgacc gccctgcagg cccaggcctt cctgaccctg 2880
accacctgtc gcaagctgct gggcctggcc gacatcgact acaccagcat catgaacgag 2940
cacttgaaca aggagaagga ggagttccgc gtgaacatcc tgccgaccct gagcaacacc 3000
ttcagcaacc cgaactacgc caaggtgaag ggcagcgacg aggacgccaa gatgatcgtg 3060
gaggctaagc cgggccacgc gttgatcggc ttcgagatca gcaacgacag catcaccgtg 3120
ctgaaggtgt acgaggccaa gctgaagcag aactaccagg tggacaagga cagcttgagc 3180
gaggtgatct acggcgacat ggacaagctg ctgtgtccgg accagagcga gcaaatctac 3240
tacaccaaca acatcgtgtt cccgaacgag tacgtgatca ccaagatcga cttcaccaag 3300
aagatgaaga ccctgcgcta cgaggtgacc gccaacttct acgacagcag caccggcgag 3360
atcgacctga acaagaagaa ggtggagagc agcgaggccg agtaccgcac cctgagcgcg 3420
aacgacgacg gcgtctacat gccactgggc gtgatcagcg agaccttcct gaccccgatc 3480
aacggctttg gcctgcaggc cgacgagaac agccgcctga tcaccctgac ctgtaagagc 3540
tacctgcgcg agctgctgct agccaccgac ctgagcaaca aggagaccaa gctgatcgtg 3600
ccaccgagcg gcttcatcag caacatcgtg gagaacggca gcatcgagga ggacaacctg 3660
gagccgtgga aggccaacaa caagaacgcc tacgtcgacc acaccggcgg cgtgaacggc 3720
accaaggccc tgtacgtgca caaggacggc ggcatcagcc agttcatcgg cgacaagctg 3780
aagccgaaga ccgagtacgt gatccagtac accgtgaagg gcaagccatc gattcacctg 3840
aaggacgaga acaccggcta catccactac gaggacacca acaacaacct ggaggactac 3900
cagaccatca acaagcgctt caccaccggc accgacctga agggcgtgta cctgatcctg 3960
aagagccaga acggcgacga ggcctggggc gacaacttca tcatcctgga gatcagcccg 4020
agcgagaagc tgctgagccc ggagctgatc aacaccaaca actggaccag caccggcagc 4080
accaacatca gcggcaacac cctgaccctg taccagggcg gccgcggcat cctgaagcag 4140
aacctgcagc tggacagctt cagcacctac cgcgtgtact tcagcgtgag cggcgacgcc 4200
aacgtgcgca tccgcaactc ccgcgaggtg ctgttcgaga agaggtacat gagcggcgcc 4260
aaggacgtga gcgagatgtt caccaccaag ttcgagaagg acaacttcta catcgagctg 4320
agccagggca acaacctgta cggcggcccg atcgtgcact tctacgacgt gagcatcaag 4380
taggagctct gtccctcact acgtccggtt cagttgcctc taatcctatc cctcgccacg 4440
ttatctgcac aaagtggagt agtcagtcat cgatcaggaa ccagacacca gacttttatt 4500
catacagtga agtgaagtga agtgcagtgc agtgagttgc tggtttttgt acaacttagt 4560
atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat 4620
ccag 4624
<210> 87
<211> 4625
<212> DNA
<213> 人工序列
<220>
<223> 23712 vip3表达盒
<400> 87
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 60
agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 120
tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 180
tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 240
gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 300
ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 360
gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 420
agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 480
taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 540
aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 600
cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 660
cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 720
acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 780
ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 840
gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 900
ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 960
cccgtcggca cctccgcttc ctaggtacgc cgctcgtcct cccccccccc ccctctctac 1020
cttctctaga tcggcgttcc ggtccatagt tagggcccgg tagttctact tctgttcatg 1080
tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga 1140
cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg 1200
ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca 1260
tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt 1320
catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt 1380
ctagatcgga gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt 1440
atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc 1500
taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt 1560
tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt 1620
agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca 1680
tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca 1740
tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg 1800
ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tattttgatc 1860
ttgatatact tggatgatgg catatccagc agctatatgt ggattttttt agccctgcct 1920
tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg 1980
tgttacttct gcaggatcct ctaagtagca gccatgaaca agaacaacac caagctgagc 2040
acccgcgccc tgccgagctt catcgactac ttcaacggca tctacggctt cgccaccggc 2100
atcaaggaca tcatgaacat gatcttcaag accgacaccg gcggcgacct gaccctggac 2160
gagatcctga agaaccagca gctgctgaac gacatcagcg gcaagctgga cggcgtgaac 2220
ggcagcctga acgacctgat cgcccagggc aacctgaaca ccgagctgag caaggagatc 2280
cttaagatcg ccaacgagca gaaccaggtg ctgaacgacg tgaacaacaa gctggacgcc 2340
atcaacacca tgctgcgcgt gtacctgccg aagatcacca gcatgctgag cgacgtgatt 2400
aagcagaact acgccctgag cctgcagatc gagtacctga gcaagcagct gcaggagatc 2460
agcgacaagc tggacatcat caacgtgaac gtcctgatca acagcaccct gaccgagatc 2520
accccggcct accagcgcat caagtacgtg aacgagaagt tcgaagagct gaccttcgcc 2580
accgagacca gcagcaaggt gaagaaggac ggcagcccgg ccgacatcct ggacgagctg 2640
accgagctga ccgagctggc gaagagcgtg accaagaacg acgtggacgg cttcgagttc 2700
tacctgaaca ccttccacga cgtgatggtg ggcaacaacc tgttcggccg cagcgccctg 2760
aagaccgcca gcgagctgat caccaaggag aacgtgaaga ccagcggcag cgaggtgggc 2820
aacgtgtaca acttcctgat cgtgctgacc gccctgcagg cccaggcctt cctgaccctg 2880
accacctgtc gcaagctgct gggcctggcc gacatcgact acaccagcat catgaacgag 2940
cacttgaaca aggagaagga ggagttccgc gtgaacatcc tgccgaccct gagcaacacc 3000
ttcagcaacc cgaactacgc caaggtgaag ggcagcgacg aggacgccaa gatgatcgtg 3060
gaggctaagc cgggccacgc gttgatcggc ttcgagatca gcaacgacag catcaccgtg 3120
ctgaaggtgt acgaggccaa gctgaagcag aactaccagg tggacaagga cagcttgagc 3180
gaggtgatct acggcgacat ggacaagctg ctgtgtccgg accagagcga gcaaatctac 3240
tacaccaaca acatcgtgtt cccgaacgag tacgtgatca ccaagatcga cttcaccaag 3300
aagatgaaga ccctgcgcta cgaggtgacc gccaacttct acgacagcag caccggcgag 3360
atcgacctga acaagaagaa ggtggagagc agcgaggccg agtaccgcac cctgagcgcg 3420
aacgacgacg gcgtctacat gccactgggc gtgatcagcg agaccttcct gaccccgatc 3480
aacggctttg gcctgcaggc cgacgagaac agccgcctga tcaccctgac ctgtaagagc 3540
tacctgcgcg agctgctgct agccaccgac ctgagcaaca aggagaccaa gctgatcgtg 3600
ccaccgagcg gcttcatcag caacatcgtg gagaacggca gcatcgagga ggacaacctg 3660
gagccgtgga aggccaacaa caagaacgcc tacgtcgacc acaccggcgg cgtgaacggc 3720
accaaggccc tgtacgtgca caaggacggc ggcatcagcc agttcatcgg cgacaagctg 3780
aagccgaaga ccgagtacgt gatccagtac accgtgaagg gcaagccatc gattcacctg 3840
aaggacgaga acaccggcta catccactac gaggacacca acaacaacct ggaggactac 3900
cagaccatca acaagcgctt caccaccggc accgacctga agggcgtgta cctgatcctg 3960
aagagccaga acggcgacga ggcctggggc gacaacttca tcatcctgga gatcagcccg 4020
agcgagaagc tgctgagccc ggagctgatc aacaccaaca actggaccag caccggcagc 4080
accaacatca gcggcaacac cctgaccctg taccagggcg gccgcggcat cctgaagcag 4140
aacctgcagc tggacagctt cagcacctac cgcgtgtact tcagcgtgag cggcgacgcc 4200
aacgtgcgca tccgcaactc ccgcgaggtg ctgttcgaga agaggtacat gagcggcgcc 4260
aaggacgtga gcgagatgtt caccaccaag ttcgagaagg acaacttcta catcgagctg 4320
agccagggca acaacctgta cggcggcccg atcgtgcact tctacgacgt gagcatcaag 4380
taggagctct atacgcacac acatgttgag atattagagg aaactgaaga attacgccac 4440
attatctgca caaagtggag tagtcagtca tcgatcagga accagacacc agacttttat 4500
tcatacagtg aagtgaagtg aagtgcagtg cagtgagttg ctggtttttg tacaacttag 4560
tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa 4620
tccag 4625
<210> 88
<211> 4635
<212> DNA
<213> 人工序列
<220>
<223> 23713 vip3表达盒
<400> 88
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 60
agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 120
tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 180
tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 240
gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 300
ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 360
gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 420
agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 480
taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 540
aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 600
cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 660
cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 720
acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 780
ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 840
gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 900
ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 960
cccgtcggca cctccgcttc ctagtatacg cacacacatg ttgagatatt agaggaaact 1020
gaagaattac gccacactag gtacgccgct cgtcctcccc ccccccccct ctctaccttc 1080
tctagatcgg cgttccggtc catagttagg gcccggtagt tctacttctg ttcatgtttg 1140
tgttagatcc gtgtttgtgt tagatccgtg ctgctagcgt tcgtacacgg atgcgacctg 1200
tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga atcctgggat 1260
ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc gttgcatagg 1320
gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg tcgggtcatc 1380
ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg gtcgttctag 1440
atcggagtag aattctgttt caaactacct ggtggattta ttaattttgg atctgtatgt 1500
gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata tcgatctagg 1560
ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct ttttgttcgc 1620
ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat cggagtagaa 1680
tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt gtgtcataca 1740
tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg tatacatgtt 1800
gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt catatgctct 1860
aaccttgagt acctatctat tataataaac aagtatgttt tataattatt ttgatcttga 1920
tatacttgga tgatggcata tccagcagct atatgtggat ttttttagcc ctgccttcat 1980
acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt gtttggtgtt 2040
acttctgcag gatcctctaa gtagcagcca tgaacaagaa caacaccaag ctgagcaccc 2100
gcgccctgcc gagcttcatc gactacttca acggcatcta cggcttcgcc accggcatca 2160
aggacatcat gaacatgatc ttcaagaccg acaccggcgg cgacctgacc ctggacgaga 2220
tcctgaagaa ccagcagctg ctgaacgaca tcagcggcaa gctggacggc gtgaacggca 2280
gcctgaacga cctgatcgcc cagggcaacc tgaacaccga gctgagcaag gagatcctta 2340
agatcgccaa cgagcagaac caggtgctga acgacgtgaa caacaagctg gacgccatca 2400
acaccatgct gcgcgtgtac ctgccgaaga tcaccagcat gctgagcgac gtgattaagc 2460
agaactacgc cctgagcctg cagatcgagt acctgagcaa gcagctgcag gagatcagcg 2520
acaagctgga catcatcaac gtgaacgtcc tgatcaacag caccctgacc gagatcaccc 2580
cggcctacca gcgcatcaag tacgtgaacg agaagttcga agagctgacc ttcgccaccg 2640
agaccagcag caaggtgaag aaggacggca gcccggccga catcctggac gagctgaccg 2700
agctgaccga gctggcgaag agcgtgacca agaacgacgt ggacggcttc gagttctacc 2760
tgaacacctt ccacgacgtg atggtgggca acaacctgtt cggccgcagc gccctgaaga 2820
ccgccagcga gctgatcacc aaggagaacg tgaagaccag cggcagcgag gtgggcaacg 2880
tgtacaactt cctgatcgtg ctgaccgccc tgcaggccca ggccttcctg accctgacca 2940
cctgtcgcaa gctgctgggc ctggccgaca tcgactacac cagcatcatg aacgagcact 3000
tgaacaagga gaaggaggag ttccgcgtga acatcctgcc gaccctgagc aacaccttca 3060
gcaacccgaa ctacgccaag gtgaagggca gcgacgagga cgccaagatg atcgtggagg 3120
ctaagccggg ccacgcgttg atcggcttcg agatcagcaa cgacagcatc accgtgctga 3180
aggtgtacga ggccaagctg aagcagaact accaggtgga caaggacagc ttgagcgagg 3240
tgatctacgg cgacatggac aagctgctgt gtccggacca gagcgagcaa atctactaca 3300
ccaacaacat cgtgttcccg aacgagtacg tgatcaccaa gatcgacttc accaagaaga 3360
tgaagaccct gcgctacgag gtgaccgcca acttctacga cagcagcacc ggcgagatcg 3420
acctgaacaa gaagaaggtg gagagcagcg aggccgagta ccgcaccctg agcgcgaacg 3480
acgacggcgt ctacatgcca ctgggcgtga tcagcgagac cttcctgacc ccgatcaacg 3540
gctttggcct gcaggccgac gagaacagcc gcctgatcac cctgacctgt aagagctacc 3600
tgcgcgagct gctgctagcc accgacctga gcaacaagga gaccaagctg atcgtgccac 3660
cgagcggctt catcagcaac atcgtggaga acggcagcat cgaggaggac aacctggagc 3720
cgtggaaggc caacaacaag aacgcctacg tcgaccacac cggcggcgtg aacggcacca 3780
aggccctgta cgtgcacaag gacggcggca tcagccagtt catcggcgac aagctgaagc 3840
cgaagaccga gtacgtgatc cagtacaccg tgaagggcaa gccatcgatt cacctgaagg 3900
acgagaacac cggctacatc cactacgagg acaccaacaa caacctggag gactaccaga 3960
ccatcaacaa gcgcttcacc accggcaccg acctgaaggg cgtgtacctg atcctgaaga 4020
gccagaacgg cgacgaggcc tggggcgaca acttcatcat cctggagatc agcccgagcg 4080
agaagctgct gagcccggag ctgatcaaca ccaacaactg gaccagcacc ggcagcacca 4140
acatcagcgg caacaccctg accctgtacc agggcggccg cggcatcctg aagcagaacc 4200
tgcagctgga cagcttcagc acctaccgcg tgtacttcag cgtgagcggc gacgccaacg 4260
tgcgcatccg caactcccgc gaggtgctgt tcgagaagag gtacatgagc ggcgccaagg 4320
acgtgagcga gatgttcacc accaagttcg agaaggacaa cttctacatc gagctgagcc 4380
agggcaacaa cctgtacggc ggcccgatcg tgcacttcta cgacgtgagc atcaagtagg 4440
agctctagat cttatctgca caaagtggag tagtcagtca tcgatcagga accagacacc 4500
agacttttat tcatacagtg aagtgaagtg aagtgcagtg cagtgagttg ctggtttttg 4560
tacaacttag tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct 4620
aaaaccaaaa tccag 4635
<210> 89
<211> 6625
<212> DNA
<213> 人工序列
<220>
<223> 23714 vip3表达盒
<400> 89
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 60
agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 120
tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 180
tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 240
gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 300
ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 360
gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 420
agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 480
taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 540
aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 600
cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 660
cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 720
acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 780
ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 840
gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 900
ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 960
cccgtcggca cctccgcttc ctaggtacgc cgctcgtcct cccccccccc ccctctctac 1020
cttctctaga tcggcgttcc ggtccatagt tagggcccgg tagttctact tctgttcatg 1080
tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga 1140
cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg 1200
ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca 1260
tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt 1320
catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt 1380
ctagatcgga gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt 1440
atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc 1500
taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt 1560
tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt 1620
agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca 1680
tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca 1740
tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg 1800
ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tattttgatc 1860
ttgatatact tggatgatgg catatccagc agctatatgt ggattttttt agccctgcct 1920
tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg 1980
tgttacttct gcaggatcct ctaagtagca gccatgaaca agaacaacac caagctgagc 2040
acccgcgccc tgccgagctt catcgactac ttcaacggca tctacggctt cgccaccggc 2100
atcaaggaca tcatgaacat gatcttcaag accgacaccg gcggcgacct gaccctggac 2160
gagatcctga agaaccagca gctgctgaac gacatcagcg gcaagctgga cggcgtgaac 2220
ggcagcctga acgacctgat cgcccagggc aacctgaaca ccgagctgag caaggagatc 2280
cttaagatcg ccaacgagca gaaccaggtg ctgaacgacg tgaacaacaa gctggacgcc 2340
atcaacacca tgctgcgcgt gtacctgccg aagatcacca gcatgctgag cgacgtgatt 2400
aagcagaact acgccctgag cctgcagatc gagtacctga gcaagcagct gcaggagatc 2460
agcgacaagc tggacatcat caacgtgaac gtcctgatca acagcaccct gaccgagatc 2520
accccggcct accagcgcat caagtacgtg aacgagaagt tcgaagagct gaccttcgcc 2580
accgagacca gcagcaaggt gaagaaggac ggcagcccgg ccgacatcct ggacgagctg 2640
accgagctga ccgagctggc gaagagcgtg accaagaacg acgtggacgg cttcgagttc 2700
tacctgaaca ccttccacga cgtgatggtg ggcaacaacc tgttcggccg cagcgccctg 2760
aagaccgcca gcgagctgat caccaaggag aacgtgaaga ccagcggcag cgaggtgggc 2820
aacgtgtaca acttcctgat cgtgctgacc gccctgcagg cccaggcctt cctgaccctg 2880
accacctgtc gcaagctgct gggcctggcc gacatcgact acaccagcat catgaacgag 2940
cacttgaaca aggagaagga ggagttccgc gtgaacatcc tgccgaccct gagcaacacc 3000
ttcagcaacc cgaactacgc caaggtgaag ggcagcgacg aggacgccaa gatgatcgtg 3060
gaggctaagc cgggccacgc gttgatcggc ttcgagatca gcaacgacag catcaccgtg 3120
ctgaaggtgt acgaggccaa gctgaagcag aactaccagg tggacaagga cagcttgagc 3180
gaggtgatct acggcgacat ggacaagctg ctgtgtccgg accagagcga gcaaatctac 3240
tacaccaaca acatcgtgtt cccgaacgag tacgtgatca ccaagatcga cttcaccaag 3300
aagatgaaga ccctgcgcta cgaggtgacc gccaacttct acgacagcag caccggcgag 3360
atcgacctga acaagaagaa ggtggagagc agcgaggccg agtaccgcac cctgagcgcg 3420
aacgacgacg gcgtctacat gccactgggc gtgatcagcg agaccttcct gaccccgatc 3480
aacggctttg gcctgcaggc cgacgagaac agccgcctga tcaccctgac ctgtaagagc 3540
tacctgcgcg agctgctgct agccaccgac ctgagcaaca aggagaccaa gctgatcgtg 3600
ccaccgagcg gcttcatcag caacatcgtg gagaacggca gcatcgagga ggacaacctg 3660
gagccgtgga aggccaacaa caagaacgcc tacgtcgacc acaccggcgg cgtgaacggc 3720
accaaggccc tgtacgtgca caaggacggc ggcatcagcc agttcatcgg cgacaagctg 3780
aagccgaaga ccgagtacgt gatccagtac accgtgaagg gcaagccatc gattcacctg 3840
aaggacgaga acaccggcta catccactac gaggacacca acaacaacct ggaggactac 3900
cagaccatca acaagcgctt caccaccggc accgacctga agggcgtgta cctgatcctg 3960
aagagccaga acggcgacga ggcctggggc gacaacttca tcatcctgga gatcagcccg 4020
agcgagaagc tgctgagccc ggagctgatc aacaccaaca actggaccag caccggcagc 4080
accaacatca gcggcaacac cctgaccctg taccagggcg gccgcggcat cctgaagcag 4140
aacctgcagc tggacagctt cagcacctac cgcgtgtact tcagcgtgag cggcgacgcc 4200
aacgtgcgca tccgcaactc ccgcgaggtg ctgttcgaga agaggtacat gagcggcgcc 4260
aaggacgtga gcgagatgtt caccaccaag ttcgagaagg acaacttcta catcgagctg 4320
agccagggca acaacctgta cggcggcccg atcgtgcact tctacgacgt gagcatcaag 4380
taggagctct atacgcacac acatgttgag atattagagg aaactgaaga attacgccac 4440
attatctgca caaagtggag tagtcagtca tcgatcagga accagacacc agacttttat 4500
tcatacagtg aagtgaagtg aagtgcagtg cagtgagttg ctggtttttg tacaacttag 4560
tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa 4620
tccaggctac acggaccgcg atcgctgacg aggctgatat aatttagggc tggttcggtt 4680
aggggtggat tgagaaggat tgaaggggat taaatatcct cctattgaaa tttgaatagg 4740
tccaatccac atcaatctat ctcaaactga ataaggcttt agggctgatt tgttgatcaa 4800
ggatcacgag agattgaaat cccctccaac accctgtgat cctcttgtca ccaaatcaat 4860
ctagggagtg tttggctccc accaccacct tctagactaa aattttagtc ttttagtttg 4920
taaactacta aacagtatga ctaagattct gtttggaggc aattaaattt ttaagaaact 4980
gatttttttt atcttttagt taggaggggc tagcttcttg gtttttaaga aactatgaat 5040
caaattttta taaactgagg cataaactga tatgtttgaa acaacctcaa tttccataaa 5100
caatttttaa aaaaattggg caaataggct ctaaatgtag tctcacaaat ataaatttta 5160
gggactaaag tgtagttctt aaagtttaga aggatgacta aagaaacaag aattccccat 5220
gctcgatttt tttttacaga gaaaaaacat ttttttaagt ttaaccaagt atatagaaaa 5280
tgttttatat ctaaataagt tcattctgaa agtatactcc actatcattc taacgataat 5340
tactacgcat cgttatttcc taatataaat tagagtgcag tgactttgta acaccgtgga 5400
gtgatgaact atttctcaaa gtttggtcat ggtgaactga actaaaaagc cagagcagct 5460
agctccacgc gagtgccaga acaaggcgcc gtgcttttgc agcttttcaa caagggtcaa 5520
gggggcagca aggcatactc tgtgtgattt gtgaagggca gcagcccggg taggtgtaac 5580
tgaaaaacag atgctgctgc tgctctcctg agtcctgaac tcccaacggt gcctgccttc 5640
tctaggaggc ctataaattt gggtcgagca gcagagcaac gccttcacag ccaatgcagg 5700
gtagcttctc acctccaaac agctagtctc tgttcttacc cattccaccc tgttacgaaa 5760
agctagaaaa gtgtcaggca ctgaaagtga gagttggagg aaagcaaacc ggctgggtat 5820
cggattcagc aacggagggt gctgccggtc ggtttgtttt cctccaatat ctcaccttca 5880
gtcgatagtc tgatgaagat gtacactacc aacatccatt catttgtgcc atctgaagtg 5940
aggattagag gcaactgaac catcatctat tgcaaaggtt cagtttcctc taatatctca 6000
tctcgaatgg gcccatcgcc ggagatgttt gtttgcatat agctgaaaat gcagtcaccc 6060
aaagtgagtg ctgaaaggaa tttctaacac ctaggttgaa ttgagtgata ctgaagtgtt 6120
tggccatctg ctagtactaa ccactagtca aacagatggc aatccacgtt gtcatcgagt 6180
tggctgaaca gatttgctcg gaaaggtata cattctccat ttgggacatg tgtgggagcc 6240
aggggatgga gcagattgga ggggatttta gcctctcgaa tcccctccat ttcgctggct 6300
cctaaacaag ccgcccttga agtgctgatt tgaggtaagt aaaccgaccg gagaagcagg 6360
gacaaagaag tatcgtttca tcagatgtga tgcaccaact tctaccgtta ttttgacaaa 6420
gcactgcatg taaatctgca gtggtttatc caatagcaaa cttgttctct actttgtcac 6480
tgtactaact gaaactgacg aacatcccca cttgtattac aattttatct cgatctgcag 6540
ggtaagacag ctttcgcgtg tatacacaca agtcgagaaa acccctcatt cgtatgcatt 6600
aacttgagag ctagaaatct agaat 6625
<210> 90
<211> 4625
<212> DNA
<213> 人工序列
<220>
<223> 23715 vip3表达盒
<400> 90
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 60
agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 120
tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 180
tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 240
gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 300
ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 360
gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 420
agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 480
taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 540
aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 600
cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 660
cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 720
acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 780
ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 840
gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 900
ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 960
cccgtcggca cctccgcttc ctaggtacgc cgctcgtcct cccccccccc ccctctctac 1020
cttctctaga tcggcgttcc ggtccatagt tagggcccgg tagttctact tctgttcatg 1080
tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga 1140
cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg 1200
ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca 1260
tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt 1320
catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt 1380
ctagatcgga gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt 1440
atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc 1500
taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt 1560
tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt 1620
agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca 1680
tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca 1740
tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg 1800
ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tattttgatc 1860
ttgatatact tggatgatgg catatccagc agctatatgt ggattttttt agccctgcct 1920
tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg 1980
tgttacttct gcaggatcct ctaagtagca gccatgaaca agaacaacac caagctgagc 2040
acccgcgccc tgccgagctt catcgactac ttcaacggca tctacggctt cgccaccggc 2100
atcaaggaca tcatgaacat gatcttcaag accgacaccg gcggcgacct gaccctggac 2160
gagatcctga agaaccagca gctgctgaac gacatcagcg gcaagctgga cggcgtgaac 2220
ggcagcctga acgacctgat cgcccagggc aacctgaaca ccgagctgag caaggagatc 2280
cttaagatcg ccaacgagca gaaccaggtg ctgaacgacg tgaacaacaa gctggacgcc 2340
atcaacacca tgctgcgcgt gtacctgccg aagatcacca gcatgctgag cgacgtgatt 2400
aagcagaact acgccctgag cctgcagatc gagtacctga gcaagcagct gcaggagatc 2460
agcgacaagc tggacatcat caacgtgaac gtcctgatca acagcaccct gaccgagatc 2520
accccggcct accagcgcat caagtacgtg aacgagaagt tcgaagagct gaccttcgcc 2580
accgagacca gcagcaaggt gaagaaggac ggcagcccgg ccgacatcct ggacgagctg 2640
accgagctga ccgagctggc gaagagcgtg accaagaacg acgtggacgg cttcgagttc 2700
tacctgaaca ccttccacga cgtgatggtg ggcaacaacc tgttcggccg cagcgccctg 2760
aagaccgcca gcgagctgat caccaaggag aacgtgaaga ccagcggcag cgaggtgggc 2820
aacgtgtaca acttcctgat cgtgctgacc gccctgcagg cccaggcctt cctgaccctg 2880
accacctgtc gcaagctgct gggcctggcc gacatcgact acaccagcat catgaacgag 2940
cacttgaaca aggagaagga ggagttccgc gtgaacatcc tgccgaccct gagcaacacc 3000
ttcagcaacc cgaactacgc caaggtgaag ggcagcgacg aggacgccaa gatgatcgtg 3060
gaggctaagc cgggccacgc gttgatcggc ttcgagatca gcaacgacag catcaccgtg 3120
ctgaaggtgt acgaggccaa gctgaagcag aactaccagg tggacaagga cagcttgagc 3180
gaggtgatct acggcgacat ggacaagctg ctgtgtccgg accagagcga gcaaatctac 3240
tacaccaaca acatcgtgtt cccgaacgag tacgtgatca ccaagatcga cttcaccaag 3300
aagatgaaga ccctgcgcta cgaggtgacc gccaacttct acgacagcag caccggcgag 3360
atcgacctga acaagaagaa ggtggagagc agcgaggccg agtaccgcac cctgagcgcg 3420
aacgacgacg gcgtctacat gccactgggc gtgatcagcg agaccttcct gaccccgatc 3480
aacggctttg gcctgcaggc cgacgagaac agccgcctga tcaccctgac ctgtaagagc 3540
tacctgcgcg agctgctgct agccaccgac ctgagcaaca aggagaccaa gctgatcgtg 3600
ccaccgagcg gcttcatcag caacatcgtg gagaacggca gcatcgagga ggacaacctg 3660
gagccgtgga aggccaacaa caagaacgcc tacgtcgacc acaccggcgg cgtgaacggc 3720
accaaggccc tgtacgtgca caaggacggc ggcatcagcc agttcatcgg cgacaagctg 3780
aagccgaaga ccgagtacgt gatccagtac accgtgaagg gcaagccatc gattcacctg 3840
aaggacgaga acaccggcta catccactac gaggacacca acaacaacct ggaggactac 3900
cagaccatca acaagcgctt caccaccggc accgacctga agggcgtgta cctgatcctg 3960
aagagccaga acggcgacga ggcctggggc gacaacttca tcatcctgga gatcagcccg 4020
agcgagaagc tgctgagccc ggagctgatc aacaccaaca actggaccag caccggcagc 4080
accaacatca gcggcaacac cctgaccctg taccagggcg gccgcggcat cctgaagcag 4140
aacctgcagc tggacagctt cagcacctac cgcgtgtact tcagcgtgag cggcgacgcc 4200
aacgtgcgca tccgcaactc ccgcgaggtg ctgttcgaga agaggtacat gagcggcgcc 4260
aaggacgtga gcgagatgtt caccaccaag ttcgagaagg acaacttcta catcgagctg 4320
agccagggca acaacctgta cggcggcccg atcgtgcact tctacgacgt gagcatcaag 4380
taggagctcc gccaaatgaa tcttcttgag attttagagt aaactgaaac tgactgttgc 4440
attatctgca caaagtggag tagtcagtca tcgatcagga accagacacc agacttttat 4500
tcatacagtg aagtgaagtg aagtgcagtg cagtgagttg ctggtttttg tacaacttag 4560
tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa 4620
tccag 4625
<210> 91
<211> 4633
<212> DNA
<213> 人工序列
<220>
<223> 23716 vip3表达盒
<400> 91
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 60
agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 120
tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 180
tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 240
gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 300
ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 360
gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 420
agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 480
taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 540
aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 600
cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 660
cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 720
acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 780
ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 840
gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 900
ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 960
cccgtcggca cctccgcttc ctaggtacgc cgctcgtcct cccccccccc ccctctctac 1020
cttctctaga tcggcgttcc ggtccatagt tagggcccgg tagttctact tctgttcatg 1080
tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga 1140
cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg 1200
ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca 1260
tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt 1320
catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt 1380
ctagatcgga gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt 1440
atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc 1500
taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt 1560
tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt 1620
agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca 1680
tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca 1740
tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg 1800
ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tattttgatc 1860
ttgatatact tggatgatgg catatccagc agctatatgt ggattttttt agccctgcct 1920
tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg 1980
tgttacttct gcaggatcct ctaagtagca gccatgtata cgcacacaca tgttgagata 2040
ttagaggaaa ctgaagaatt acgccacatt aacaagaaca acaccaagct gagcacccgc 2100
gccctgccga gcttcatcga ctacttcaac ggcatctacg gcttcgccac cggcatcaag 2160
gacatcatga acatgatctt caagaccgac accggcggcg acctgaccct ggacgagatc 2220
ctgaagaacc agcagctgct gaacgacatc agcggcaagc tggacggcgt gaacggcagc 2280
ctgaacgacc tgatcgccca gggcaacctg aacaccgagc tgagcaagga gatccttaag 2340
atcgccaacg agcagaacca ggtgctgaac gacgtgaaca acaagctgga cgccatcaac 2400
accatgctgc gcgtgtacct gccgaagatc accagcatgc tgagcgacgt gattaagcag 2460
aactacgccc tgagcctgca gatcgagtac ctgagcaagc agctgcagga gatcagcgac 2520
aagctggaca tcatcaacgt gaacgtcctg atcaacagca ccctgaccga gatcaccccg 2580
gcctaccagc gcatcaagta cgtgaacgag aagttcgaag agctgacctt cgccaccgag 2640
accagcagca aggtgaagaa ggacggcagc ccggccgaca tcctggacga gctgaccgag 2700
ctgaccgagc tggcgaagag cgtgaccaag aacgacgtgg acggcttcga gttctacctg 2760
aacaccttcc acgacgtgat ggtgggcaac aacctgttcg gccgcagcgc cctgaagacc 2820
gccagcgagc tgatcaccaa ggagaacgtg aagaccagcg gcagcgaggt gggcaacgtg 2880
tacaacttcc tgatcgtgct gaccgccctg caggcccagg ccttcctgac cctgaccacc 2940
tgtcgcaagc tgctgggcct ggccgacatc gactacacca gcatcatgaa cgagcacttg 3000
aacaaggaga aggaggagtt ccgcgtgaac atcctgccga ccctgagcaa caccttcagc 3060
aacccgaact acgccaaggt gaagggcagc gacgaggacg ccaagatgat cgtggaggct 3120
aagccgggcc acgcgttgat cggcttcgag atcagcaacg acagcatcac cgtgctgaag 3180
gtgtacgagg ccaagctgaa gcagaactac caggtggaca aggacagctt gagcgaggtg 3240
atctacggcg acatggacaa gctgctgtgt ccggaccaga gcgagcaaat ctactacacc 3300
aacaacatcg tgttcccgaa cgagtacgtg atcaccaaga tcgacttcac caagaagatg 3360
aagaccctgc gctacgaggt gaccgccaac ttctacgaca gcagcaccgg cgagatcgac 3420
ctgaacaaga agaaggtgga gagcagcgag gccgagtacc gcaccctgag cgcgaacgac 3480
gacggcgtct acatgccact gggcgtgatc agcgagacct tcctgacccc gatcaacggc 3540
tttggcctgc aggccgacga gaacagccgc ctgatcaccc tgacctgtaa gagctacctg 3600
cgcgagctgc tgctagccac cgacctgagc aacaaggaga ccaagctgat cgtgccaccg 3660
agcggcttca tcagcaacat cgtggagaac ggcagcatcg aggaggacaa cctggagccg 3720
tggaaggcca acaacaagaa cgcctacgtc gaccacaccg gcggcgtgaa cggcaccaag 3780
gccctgtacg tgcacaagga cggcggcatc agccagttca tcggcgacaa gctgaagccg 3840
aagaccgagt acgtgatcca gtacaccgtg aagggcaagc catcgattca cctgaaggac 3900
gagaacaccg gctacatcca ctacgaggac accaacaaca acctggagga ctaccagacc 3960
atcaacaagc gcttcaccac cggcaccgac ctgaagggcg tgtacctgat cctgaagagc 4020
cagaacggcg acgaggcctg gggcgacaac ttcatcatcc tggagatcag cccgagcgag 4080
aagctgctga gcccggagct gatcaacacc aacaactgga ccagcaccgg cagcaccaac 4140
atcagcggca acaccctgac cctgtaccag ggcggccgcg gcatcctgaa gcagaacctg 4200
cagctggaca gcttcagcac ctaccgcgtg tacttcagcg tgagcggcga cgccaacgtg 4260
cgcatccgca actcccgcga ggtgctgttc gagaagaggt acatgagcgg cgccaaggac 4320
gtgagcgaga tgttcaccac caagttcgag aaggacaact tctacatcga gctgagccag 4380
ggcaacaacc tgtacggcgg cccgatcgtg cacttctacg acgtgagcat caagtaggag 4440
ctctagatct tatctgcaca aagtggagta gtcagtcatc gatcaggaac cagacaccag 4500
acttttattc atacagtgaa gtgaagtgaa gtgcagtgca gtgagttgct ggtttttgta 4560
caacttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa 4620
aaccaaaatc cag 4633
<210> 92
<211> 4603
<212> DNA
<213> 人工序列
<220>
<223> 23717 vip3表达盒
<400> 92
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 60
agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 120
tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 180
tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 240
gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 300
ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 360
gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 420
agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 480
taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 540
aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 600
cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 660
cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 720
acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 780
ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 840
gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 900
ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 960
cccgtcggca cctccgcttc ctaggtacgc cgctcgtcct cccccccccc ccctctctac 1020
cttctctaga tcggcgttcc ggtccatagt tagggcccgg tagttctact tctgttcatg 1080
tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga 1140
cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg 1200
ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca 1260
tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt 1320
catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt 1380
ctagatcgga gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt 1440
atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc 1500
taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt 1560
tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt 1620
agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca 1680
tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca 1740
tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg 1800
ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tattttgatc 1860
ttgatatact tggatgatgg catatccagc agctatatgt ggattttttt agccctgcct 1920
tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg 1980
tgttacttct gcaggatcct ctaagtagca gccatggctg agatattaga ggaaactgaa 2040
aacaagaaca acaccaagct gagcacccgc gccctgccga gcttcatcga ctacttcaac 2100
ggcatctacg gcttcgccac cggcatcaag gacatcatga acatgatctt caagaccgac 2160
accggcggcg acctgaccct ggacgagatc ctgaagaacc agcagctgct gaacgacatc 2220
agcggcaagc tggacggcgt gaacggcagc ctgaacgacc tgatcgccca gggcaacctg 2280
aacaccgagc tgagcaagga gatccttaag atcgccaacg agcagaacca ggtgctgaac 2340
gacgtgaaca acaagctgga cgccatcaac accatgctgc gcgtgtacct gccgaagatc 2400
accagcatgc tgagcgacgt gattaagcag aactacgccc tgagcctgca gatcgagtac 2460
ctgagcaagc agctgcagga gatcagcgac aagctggaca tcatcaacgt gaacgtcctg 2520
atcaacagca ccctgaccga gatcaccccg gcctaccagc gcatcaagta cgtgaacgag 2580
aagttcgaag agctgacctt cgccaccgag accagcagca aggtgaagaa ggacggcagc 2640
ccggccgaca tcctggacga gctgaccgag ctgaccgagc tggcgaagag cgtgaccaag 2700
aacgacgtgg acggcttcga gttctacctg aacaccttcc acgacgtgat ggtgggcaac 2760
aacctgttcg gccgcagcgc cctgaagacc gccagcgagc tgatcaccaa ggagaacgtg 2820
aagaccagcg gcagcgaggt gggcaacgtg tacaacttcc tgatcgtgct gaccgccctg 2880
caggcccagg ccttcctgac cctgaccacc tgtcgcaagc tgctgggcct ggccgacatc 2940
gactacacca gcatcatgaa cgagcacttg aacaaggaga aggaggagtt ccgcgtgaac 3000
atcctgccga ccctgagcaa caccttcagc aacccgaact acgccaaggt gaagggcagc 3060
gacgaggacg ccaagatgat cgtggaggct aagccgggcc acgcgttgat cggcttcgag 3120
atcagcaacg acagcatcac cgtgctgaag gtgtacgagg ccaagctgaa gcagaactac 3180
caggtggaca aggacagctt gagcgaggtg atctacggcg acatggacaa gctgctgtgt 3240
ccggaccaga gcgagcaaat ctactacacc aacaacatcg tgttcccgaa cgagtacgtg 3300
atcaccaaga tcgacttcac caagaagatg aagaccctgc gctacgaggt gaccgccaac 3360
ttctacgaca gcagcaccgg cgagatcgac ctgaacaaga agaaggtgga gagcagcgag 3420
gccgagtacc gcaccctgag cgcgaacgac gacggcgtct acatgccact gggcgtgatc 3480
agcgagacct tcctgacccc gatcaacggc tttggcctgc aggccgacga gaacagccgc 3540
ctgatcaccc tgacctgtaa gagctacctg cgcgagctgc tgctagccac cgacctgagc 3600
aacaaggaga ccaagctgat cgtgccaccg agcggcttca tcagcaacat cgtggagaac 3660
ggcagcatcg aggaggacaa cctggagccg tggaaggcca acaacaagaa cgcctacgtc 3720
gaccacaccg gcggcgtgaa cggcaccaag gccctgtacg tgcacaagga cggcggcatc 3780
agccagttca tcggcgacaa gctgaagccg aagaccgagt acgtgatcca gtacaccgtg 3840
aagggcaagc catcgattca cctgaaggac gagaacaccg gctacatcca ctacgaggac 3900
accaacaaca acctggagga ctaccagacc atcaacaagc gcttcaccac cggcaccgac 3960
ctgaagggcg tgtacctgat cctgaagagc cagaacggcg acgaggcctg gggcgacaac 4020
ttcatcatcc tggagatcag cccgagcgag aagctgctga gcccggagct gatcaacacc 4080
aacaactgga ccagcaccgg cagcaccaac atcagcggca acaccctgac cctgtaccag 4140
ggcggccgcg gcatcctgaa gcagaacctg cagctggaca gcttcagcac ctaccgcgtg 4200
tacttcagcg tgagcggcga cgccaacgtg cgcatccgca actcccgcga ggtgctgttc 4260
gagaagaggt acatgagcgg cgccaaggac gtgagcgaga tgttcaccac caagttcgag 4320
aaggacaact tctacatcga gctgagccag ggcaacaacc tgtacggcgg cccgatcgtg 4380
cacttctacg acgtgagcat caagtaggag ctctagatct tatctgcaca aagtggagta 4440
gtcagtcatc gatcaggaac cagacaccag acttttattc atacagtgaa gtgaagtgaa 4500
gtgcagtgca gtgagttgct ggtttttgta caacttagta tgtatttgta tttgtaaaat 4560
acttctatca ataaaatttc taattcctaa aaccaaaatc cag 4603
<210> 93
<211> 4591
<212> DNA
<213> 人工序列
<220>
<223> 23718 vip3表达盒
<400> 93
ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 60
agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 120
tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 180
tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 240
gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 300
ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 360
gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 420
agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 480
taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 540
aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 600
cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 660
cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 720
acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 780
ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 840
gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 900
ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 960
cccgtcggca cctccgcttc ctaggtacgc cgctcgtcct cccccccccc ccctctctac 1020
cttctctaga tcggcgttcc ggtccatagt tagggcccgg tagttctact tctgttcatg 1080
tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga 1140
cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg 1200
ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca 1260
tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt 1320
catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt 1380
ctagatcgga gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt 1440
atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc 1500
taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt 1560
tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt 1620
agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca 1680
tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca 1740
tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg 1800
ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tattttgatc 1860
ttgatatact tggatgatgg catatccagc agctatatgt ggattttttt agccctgcct 1920
tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg 1980
tgttacttct gcaggatcct ctaagtagca gccatgaaca agaacaacac caagctgagc 2040
acccgcgccc tgccgagctt catcgactac ttcaacggca tctacggctt cgccaccggc 2100
atcaaggaca tcatgaacat gatcttcaag accgacaccg gcggcgacct gaccctggac 2160
gagatcctga agaaccagca gctgctgaac gacatcagcg gcaagctgga cggcgtgaac 2220
ggcagcctga acgacctgat cgcccagggc aacctgaaca ccgagctgag caaggagatc 2280
cttaagatcg ccaacgagca gaaccaggtg ctgaacgacg tgaacaacaa gctggacgcc 2340
atcaacacca tgctgcgcgt gtacctgccg aagatcacca gcatgctgag cgacgtgatt 2400
aagcagaact acgccctgag cctgcagatc gagtacctga gcaagcagct gcaggagatc 2460
agcgacaagc tggacatcat caacgtgaac gtcctgatca acagcaccct gaccgagatc 2520
accccggcct accagcgcat caagtacgtg aacgagaagt tcgaagagct gaccttcgcc 2580
accgagacca gcagcgctga gatattagag gaaactgaag acggcagccc ggccgacatc 2640
ctggacgagc tgaccgagct gaccgagctg gcgaagagcg tgaccaagaa cgacgtggac 2700
ggcttcgagt tctacctgaa caccttccac gacgtgatgg tgggcaacaa cctgttcggc 2760
cgcagcgccc tgaagaccgc cagcgagctg atcaccaagg agaacgtgaa gaccagcggc 2820
agcgaggtgg gcaacgtgta caacttcctg atcgtgctga ccgccctgca ggcccaggcc 2880
ttcctgaccc tgaccacctg tcgcaagctg ctgggcctgg ccgacatcga ctacaccagc 2940
atcatgaacg agcacttgaa caaggagaag gaggagttcc gcgtgaacat cctgccgacc 3000
ctgagcaaca ccttcagcaa cccgaactac gccaaggtga agggcagcga cgaggacgcc 3060
aagatgatcg tggaggctaa gccgggccac gcgttgatcg gcttcgagat cagcaacgac 3120
agcatcaccg tgctgaaggt gtacgaggcc aagctgaagc agaactacca ggtggacaag 3180
gacagcttga gcgaggtgat ctacggcgac atggacaagc tgctgtgtcc ggaccagagc 3240
gagcaaatct actacaccaa caacatcgtg ttcccgaacg agtacgtgat caccaagatc 3300
gacttcacca agaagatgaa gaccctgcgc tacgaggtga ccgccaactt ctacgacagc 3360
agcaccggcg agatcgacct gaacaagaag aaggtggaga gcagcgaggc cgagtaccgc 3420
accctgagcg cgaacgacga cggcgtctac atgccactgg gcgtgatcag cgagaccttc 3480
ctgaccccga tcaacggctt tggcctgcag gccgacgaga acagccgcct gatcaccctg 3540
acctgtaaga gctacctgcg cgagctgctg ctagccaccg acctgagcaa caaggagacc 3600
aagctgatcg tgccaccgag cggcttcatc agcaacatcg tggagaacgg cagcatcgag 3660
gaggacaacc tggagccgtg gaaggccaac aacaagaacg cctacgtcga ccacaccggc 3720
ggcgtgaacg gcaccaaggc cctgtacgtg cacaaggacg gcggcatcag ccagttcatc 3780
ggcgacaagc tgaagccgaa gaccgagtac gtgatccagt acaccgtgaa gggcaagcca 3840
tcgattcacc tgaaggacga gaacaccggc tacatccact acgaggacac caacaacaac 3900
ctggaggact accagaccat caacaagcgc ttcaccaccg gcaccgacct gaagggcgtg 3960
tacctgatcc tgaagagcca gaacggcgac gaggcctggg gcgacaactt catcatcctg 4020
gagatcagcc cgagcgagaa gctgctgagc ccggagctga tcaacaccaa caactggacc 4080
agcaccggca gcaccaacat cagcggcaac accctgaccc tgtaccaggg cggccgcggc 4140
atcctgaagc agaacctgca gctggacagc ttcagcacct accgcgtgta cttcagcgtg 4200
agcggcgacg ccaacgtgcg catccgcaac tcccgcgagg tgctgttcga gaagaggtac 4260
atgagcggcg ccaaggacgt gagcgagatg ttcaccacca agttcgagaa ggacaacttc 4320
tacatcgagc tgagccaggg caacaacctg tacggcggcc cgatcgtgca cttctacgac 4380
gtgagcatca agtaggagct ctagatctta tctgcacaaa gtggagtagt cagtcatcga 4440
tcaggaacca gacaccagac ttttattcat acagtgaagt gaagtgaagt gcagtgcagt 4500
gagttgctgg tttttgtaca acttagtatg tatttgtatt tgtaaaatac ttctatcaat 4560
aaaatttcta attcctaaaa ccaaaatcca g 4591
<210> 94
<211> 22
<212> RNA
<213> 人工序列
<220>
<223> miR2275a-3p起始子序列
<400> 94
ugagauauug gaggaaaaca aa 22
<210> 95
<211> 21
<212> RNA
<213> 人工序列
<220>
<223> miR2275b-5p起始子序列
<400> 95
gguucaguug ccucuaaucc u 21
<210> 96
<211> 22
<212> RNA
<213> 人工序列
<220>
<223> miR2275b-3p起始子序列
<400> 96
ugagauauua gaggaaacug aa 22
<210> 97
<211> 22
<212> RNA
<213> 人工序列
<220>
<223> miR2275b-3p PHAS靶起始子序列
<400> 97
ugagauuuua gaguaaacug aa 22
<210> 98
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> miR2275a-3p起始子序列
<400> 98
tgagatattg gaggaaaaca aa 22
<210> 99
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> miR2275b-5p起始子序列
<400> 99
ggttcagttg cctctaatcc t 21
<210> 100
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> miR2275b-3p起始子序列
<400> 100
tgagatatta gaggaaactg aa 22
<210> 101
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> miR2275b-3p PHAS靶起始子序列
<400> 101
tgagatttta gagtaaactg aa 22
<210> 102
<211> 22
<212> RNA
<213> 玉蜀黍
<400> 102
uucaguuucc ucuaauaucu ca 22
<210> 103
<211> 21
<212> RNA
<213> 玉蜀黍
<400> 103
aggauuagag gcaacugaac c 21
<210> 104
<211> 14454
<212> DNA
<213> 人工序列
<220>
<223> 23705载体
<400> 104
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta 1260
gatcggcgtt ccggtccata gttagggccc ggtagttcta cttctgttca tgtttgtgtt 1320
agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg 1380
tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct 1440
ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt 1500
ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt 1560
catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg 1620
gagtagaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt 1680
gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag 1740
gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg 1800
ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact 1860
gtttcaaact acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt 1920
catagttacg agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg 1980
tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc 2040
ttgagtacct atctattata ataaacaagt atgttttata attattttga tcttgatata 2100
cttggatgat ggcatatcca gcagctatat gtggattttt ttagccctgc cttcatacgc 2160
tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt 2220
ctgcaggatc ctctaagtag cagccatgaa caagaacaac accaagctga gcacccgcgc 2280
cctgccgagc ttcatcgact acttcaacgg catctacggc ttcgccaccg gcatcaagga 2340
catcatgaac atgatcttca agaccgacac cggcggcgac ctgaccctgg acgagatcct 2400
gaagaaccag cagctgctga acgacatcag cggcaagctg gacggcgtga acggcagcct 2460
gaacgacctg atcgcccagg gcaacctgaa caccgagctg agcaaggaga tccttaagat 2520
cgccaacgag cagaaccagg tgctgaacga cgtgaacaac aagctggacg ccatcaacac 2580
catgctgcgc gtgtacctgc cgaagatcac cagcatgctg agcgacgtga ttaagcagaa 2640
ctacgccctg agcctgcaga tcgagtacct gagcaagcag ctgcaggaga tcagcgacaa 2700
gctggacatc atcaacgtga acgtcctgat caacagcacc ctgaccgaga tcaccccggc 2760
ctaccagcgc atcaagtacg tgaacgagaa gttcgaagag ctgaccttcg ccaccgagac 2820
cagcagcaag gtgaagaagg acggcagccc ggccgacatc ctggacgagc tgaccgagct 2880
gaccgagctg gcgaagagcg tgaccaagaa cgacgtggac ggcttcgagt tctacctgaa 2940
caccttccac gacgtgatgg tgggcaacaa cctgttcggc cgcagcgccc tgaagaccgc 3000
cagcgagctg atcaccaagg agaacgtgaa gaccagcggc agcgaggtgg gcaacgtgta 3060
caacttcctg atcgtgctga ccgccctgca ggcccaggcc ttcctgaccc tgaccacctg 3120
tcgcaagctg ctgggcctgg ccgacatcga ctacaccagc atcatgaacg agcacttgaa 3180
caaggagaag gaggagttcc gcgtgaacat cctgccgacc ctgagcaaca ccttcagcaa 3240
cccgaactac gccaaggtga agggcagcga cgaggacgcc aagatgatcg tggaggctaa 3300
gccgggccac gcgttgatcg gcttcgagat cagcaacgac agcatcaccg tgctgaaggt 3360
gtacgaggcc aagctgaagc agaactacca ggtggacaag gacagcttga gcgaggtgat 3420
ctacggcgac atggacaagc tgctgtgtcc ggaccagagc gagcaaatct actacaccaa 3480
caacatcgtg ttcccgaacg agtacgtgat caccaagatc gacttcacca agaagatgaa 3540
gaccctgcgc tacgaggtga ccgccaactt ctacgacagc agcaccggcg agatcgacct 3600
gaacaagaag aaggtggaga gcagcgaggc cgagtaccgc accctgagcg cgaacgacga 3660
cggcgtctac atgccactgg gcgtgatcag cgagaccttc ctgaccccga tcaacggctt 3720
tggcctgcag gccgacgaga acagccgcct gatcaccctg acctgtaaga gctacctgcg 3780
cgagctgctg ctagccaccg acctgagcaa caaggagacc aagctgatcg tgccaccgag 3840
cggcttcatc agcaacatcg tggagaacgg cagcatcgag gaggacaacc tggagccgtg 3900
gaaggccaac aacaagaacg cctacgtcga ccacaccggc ggcgtgaacg gcaccaaggc 3960
cctgtacgtg cacaaggacg gcggcatcag ccagttcatc ggcgacaagc tgaagccgaa 4020
gaccgagtac gtgatccagt acaccgtgaa gggcaagcca tcgattcacc tgaaggacga 4080
gaacaccggc tacatccact acgaggacac caacaacaac ctggaggact accagaccat 4140
caacaagcgc ttcaccaccg gcaccgacct gaagggcgtg tacctgatcc tgaagagcca 4200
gaacggcgac gaggcctggg gcgacaactt catcatcctg gagatcagcc cgagcgagaa 4260
gctgctgagc ccggagctga tcaacaccaa caactggacc agcaccggca gcaccaacat 4320
cagcggcaac accctgaccc tgtaccaggg cggccgcggc atcctgaagc agaacctgca 4380
gctggacagc ttcagcacct accgcgtgta cttcagcgtg agcggcgacg ccaacgtgcg 4440
catccgcaac tcccgcgagg tgctgttcga gaagaggtac atgagcggcg ccaaggacgt 4500
gagcgagatg ttcaccacca agttcgagaa ggacaacttc tacatcgagc tgagccaggg 4560
caacaacctg tacggcggcc cgatcgtgca cttctacgac gtgagcatca agtaggagct 4620
ctagatctta tctgcacaaa gtggagtagt cagtcatcga tcaggaacca gacaccagac 4680
ttttattcat acagtgaagt gaagtgaagt gcagtgcagt gagttgctgg tttttgtaca 4740
acttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa 4800
ccaaaatcca ggctacacgg accgcgatcg cttaattaag cttgcatgcc tgcagtgcag 4860
cgtgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa gttataaaaa 4920
attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat ctttatacat 4980
atatttaaac tttactctac gaataatata atctatagta ctacaataat atcagtgttt 5040
tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag tattttgaca 5100
acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt tttgcaaata 5160
gcttcaccta tataatactt catccatttt attagtacat ccatttaggg tttagggtta 5220
atggttttta tagactaatt tttttagtac atctatttta ttctatttta gcctctaaat 5280
taagaaaact aaaactctat tttagttttt ttatttaata atttagatat aaaatagaat 5340
aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa actaaggaaa 5400
catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgccgac gagtctaacg 5460
gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac ggcacggcat 5520
ctctgtcgct gcctctggac ccctctcgag agttccgctc caccgttgga cttgctccgc 5580
tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg agccggcacg gcaggcggcc 5640
tcctcctcct ctcacggcac cggcagctac gggggattcc tttcccaccg ctccttcgct 5700
ttcccttcct cgcccgccgt aataaataga caccccctcc acaccctctt tccccaacct 5760
cgtgttgttc ggagcgcaca cacacacaac cagatctccc ccaaatccac ccgtcggcac 5820
ctccgcttca aggtacgccg ctcgtcctcc cccccccccc ctctctacct tctctagatc 5880
ggcgttccgg tccatagtta gggcccggta gttctacttc tgttcatgtt tgtgttagat 5940
ccgtgtttgt gttagatccg tgctgttagc gttcgtacac ggatgcgacc tgtacgtcag 6000
acacgttctg attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag 6060
ccgttccgca gacgggatcg atttcatgat tttttttgtt tcgttgcata gggtttggtt 6120
tgcccttttc ctttatttca atatatgccg tgcacttgtt tgtcgggtca tcttttcatg 6180
cttttttttg tcttggttgt gatgatgtgg tctggttggg cggtcgttct agatcggagt 6240
agaattctgt ttcaaactac ctggtggatt tattaatttt ggatctgtat gtgtgtgcca 6300
tacatattca tagttacgaa ttgaagatga tggatggaaa tatcgatcta ggataggtat 6360
acatgttgat gcgggtttta ctgatgcata tacagagatg ctttttgttc gcttggttgt 6420
gatgatgtgg tgtggttggg cggtcgttca ttcgttctag atcggagtag aatactgttt 6480
caaactacct ggtgtattta ttaattttgg aactgtatgt gtgtgtcata catcttcata 6540
gttacgagtt taagatggat ggaaatatcg atctaggata ggtatacatg ttgatgtggg 6600
ttttactgat gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga 6660
gtacctatct attataataa acaagtatgt tttataatta ttttgatctt gatatacttg 6720
gatgatggca tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt 6780
tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc 6840
agggatctcc gatcatgcaa aaactcatta actcagtgca aaactatgcc tggggcagca 6900
aaacggcgtt gactgaactt tatggtatgg aaaatccgtc cagccagccg atggccgagc 6960
tgtggatggg cgcacatccg aaaagcagtt cacgagtgca gaatgccgcc ggagatatcg 7020
tttcactgcg tgatgtgatt gagagtgata aatcgactct gctcggagag gccgttgcca 7080
aacgctttgg cgaactgcct ttcctgttca aagtattatg cgcagcacag ccactctcca 7140
ttcaggttca tccaaacaaa cacaattctg aaatcggttt tgccaaagaa aatgccgcag 7200
gtatcccgat ggatgccgcc gagcgtaact ataaagatcc taaccacaag ccggagctgg 7260
tttttgcgct gacgcctttc cttgcgatga acgcgtttcg tgaattttcc gagattgtct 7320
ccctactcca gccggtcgca ggtgcacatc cggcgattgc tcacttttta caacagcctg 7380
atgccgaacg tttaagcgaa ctgttcgcca gcctgttgaa tatgcagggt gaagaaaaat 7440
cccgcgcgct ggcgatttta aaatcggccc tcgatagcca gcagggtgaa ccgtggcaaa 7500
cgattcgttt aatttctgaa ttttacccgg aagacagcgg tctgttctcc ccgctattgc 7560
tgaatgtggt gaaattgaac cctggcgaag cgatgttcct gttcgctgaa acaccgcacg 7620
cttacctgca aggcgtggcg ctggaagtga tggcaaactc cgataacgtg ctgcgtgcgg 7680
gtctgacgcc taaatacatt gatattccgg aactggttgc caatgtgaaa ttcgaagcca 7740
aaccggctaa ccagttgttg acccagccgg tgaaacaagg tgcagaactg gacttcccga 7800
ttccagtgga tgattttgcc ttctcgctgc atgaccttag tgataaagaa accaccatta 7860
gccagcagag tgccgccatt ttgttctgcg tcgaaggcga tgcaacgttg tggaaaggtt 7920
ctcagcagtt acagcttaaa ccgggtgaat cagcgtttat tgccgccaac gaatcaccgg 7980
tgactgtcaa aggccacggc cgtttagcgc gtgtttacaa caagctgtaa gagcttactg 8040
aaaaaattaa catctcttgc taagctgggt catgggtcgt ttaagctgcc gatgtgcctg 8100
cgtcgtctgg tgccctctct ccatatggag gttgtcaaag tatctgctgt tcgtgtcatg 8160
agtcgtgtca gtgttggttt aataatggac cggttgtgtt gtgtgtgcgt actacccaga 8220
actatgacaa atcatgaata agtttgatgt ttgaaattaa agcctgtgct cattatgttc 8280
tgtctttcag ttgtctccta atatttgcct ccaggtactg gctatctacc gtttcttact 8340
taggaggtgt ttgaatgcac taaaactaat agttagtggc taaaattagt taaaacatcc 8400
aaacaccata gctaatagtt gaactattag ctatttttgg aaaattagtt aatagtgagg 8460
tagttatttg ttagctagct aattcaacta acaattttta gccaactaac aattagtttc 8520
agtgcattca aacaccccct taatgttaac gtggttctat ctaccgtctc ctaatatatg 8580
gttgattgtt cggtttgttg ctatgctatt gggttctgat tgctgctagt tcttgctgaa 8640
tccagaagtt ctcgtagtat agctcagatt catattattt atttgagtga taagtgatcc 8700
aggttattac tatgttagct aggttttttt tacaaggata aattatctgt gatcataatt 8760
cttatgaaag ctttatgttt cctggaggca gtggcatgca atgcatgaca gcaacttgat 8820
cacaccagct gaggtagata cggtaacaag gttcttaaat ctgttcacca aatcattgga 8880
gaacacacat acacattctt gccagtcttg gttagagaaa tttcatgaca aaatgccaaa 8940
gctgtcttga ctcttcactt ttggccatga gtcgtgactt agtttggttt aatggaccgg 9000
ttctcctagc ttgttctact caaaactgtt gttgatgcga ataagttgtg atggttgatc 9060
tctggatttt gttttgctct caatagtgga cgagattaga tagcctgcag gcccgggggc 9120
gcgccctaat tagctaacgg ccaggatcgc cgcgtgagcc tttagcaact agctagatta 9180
attaacgcaa tctgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc 9240
tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata 9300
caggcagccc atcagaatta attctcatgt ttgacagctt atcatcgact gcacggtgca 9360
ccaatgcttc tggcgtcagg cagccatcgg aagctgtggt atggctgtgc aggtcgtaaa 9420
tcactgcata attcgtgtcg ctcaaggcgc actcccgttc tggataatgt tttttgcgcc 9480
gacatcataa cggttctggc aaatattctg aaatgagctg ttgacaatta atcatccggc 9540
tcgtataatg tgtggaattg tgagcggata acaatttcac acaggaaaca gaccatgagg 9600
gaagcgttga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc 9660
catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg 9720
aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg 9780
cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt 9840
ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca 9900
gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc 9960
gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc 10020
gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta 10080
tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat 10140
gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc 10200
gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc 10260
gtcatacttg aagctaggca ggcttatctt ggacaagaag atcgcttggc ctcgcgcgca 10320
gatcagttgg aagaatttgt tcactacgtg aaaggcgaga tcaccaaagt agtcggcaaa 10380
taaagctcta gtggatctcc gtacccaggg atctggctcg cggcggacgc acgacgccgg 10440
ggcgagacca taggcgatct cctaaatcaa tagtagctgt aacctcgaag cgtttcactt 10500
gtaacaacga ttgagaattt ttgtcataaa attgaaatac ttggttcgca tttttgtcat 10560
ccgcggtcag ccgcaattct gacgaactgc ccatttagct ggagatgatt gtacatcctt 10620
cacgtgaaaa tttctcaagc gctgtgaaca agggttcaga ttttagattg aaaggtgagc 10680
cgttgaaaca cgttcttctt gtcgatgacg acgtcgctat gcggcatctt attattgaat 10740
accttacgat ccacgccttc aaagtgaccg cggtagccga cagcacccag ttcacaagag 10800
tactctcttc cgcgacggtc gatgtcgtgg ttgttgatct agatttaggt cgtgaagatg 10860
ggctcgagat cgttcgtaat ctggcggcaa agtctgatat tccaatcata attatcagtg 10920
gcgaccgcct tgaggagacg gataaagttg ttgcactcga gctaggagca agtgatttta 10980
tcgctaagcc gttcagtatc agagagtttc tagcacgcat tcgggttgcc ttgcgcgtgc 11040
gccccaacgt tgtccgctcc aaagaccgac ggtctttttg ttttactgac tggacactta 11100
atctcaggca acgtcgcttg atgtccgaag ctggcggtga ggtgaaactt acggcaggtg 11160
agttcaatct tctcctcgcg tttttagaga aaccccgcga cgttctatcg cgcgagcaac 11220
ttctcattgc cagtcgagta cgcgacgagg aggtttatga caggagtata gatgttctca 11280
ttttgaggct gcgccgcaaa cttgaggcag atccgtcaag ccctcaactg ataaaaacag 11340
caagaggtgc cggttatttc tttgacgcgg acgtgcaggt ttcgcacggg gggacgatgg 11400
cagcctgagc caattcccag atccccgagg aatcggcgtg agcggtcgca aaccatccgg 11460
cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca 11520
ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc 11580
cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag 11640
gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg 11700
cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg 11760
acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg 11820
gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct 11880
aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg 11940
tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga 12000
cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa 12060
gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta 12120
caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tggctgattg 12180
gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta 12240
ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg 12300
caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga 12360
gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta 12420
cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct 12480
gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc 12540
cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa 12600
cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg 12660
gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta 12720
aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg 12780
ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg 12840
ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg 12900
gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgctg 12960
aggtctgcct cgtgaagaag gtgttgctga ctcataccag gcctgaatcg ccccatcatc 13020
cagccagaaa gtgagggagc cacggttgat gagagctttg ttgtaggtgg accagttggt 13080
gattttgaac ttttgctttg ccacggaacg gtctgcgttg tcgggaagat gcgtgatctg 13140
atccttcaac tcagcaaaag ttcgatttat tcaacaaagc cgccgtcccg tcaagtcagc 13200
gtaatgctct gccagtgtta caaccaatta accaattctg attagaaaaa ctcatcgagc 13260
atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc 13320
cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg 13380
tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca 13440
aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc 13500
aaaagctctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 13560
ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 13620
atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 13680
gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 13740
gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 13800
gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 13860
gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 13920
aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 13980
ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 14040
taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 14100
tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 14160
gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 14220
taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 14280
tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 14340
tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 14400
ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttgatccgga atta 14454
<210> 105
<211> 14500
<212> DNA
<213> 人工序列
<220>
<223> 23708载体
<400> 105
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta 1260
gatcggcgtt ccggtccata gttagggccc ggtagttcta cttctgttca tgtttgtgtt 1320
agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg 1380
tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct 1440
ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt 1500
ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt 1560
catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg 1620
gagtagaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt 1680
gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag 1740
gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg 1800
ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact 1860
gtttcaaact acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt 1920
catagttacg agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg 1980
tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc 2040
ttgagtacct atctattata ataaacaagt atgttttata attattttga tcttgatata 2100
cttggatgat ggcatatcca gcagctatat gtggattttt ttagccctgc cttcatacgc 2160
tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt 2220
ctgcaggatc ctctaagtag cagccatgaa caagaacaac accaagctga gcacccgcgc 2280
cctgccgagc ttcatcgact acttcaacgg catctacggc ttcgccaccg gcatcaagga 2340
catcatgaac atgatcttca agaccgacac cggcggcgac ctgaccctgg acgagatcct 2400
gaagaaccag cagctgctga acgacatcag cggcaagctg gacggcgtga acggcagcct 2460
gaacgacctg atcgcccagg gcaacctgaa caccgagctg agcaaggaga tccttaagat 2520
cgccaacgag cagaaccagg tgctgaacga cgtgaacaac aagctggacg ccatcaacac 2580
catgctgcgc gtgtacctgc cgaagatcac cagcatgctg agcgacgtga ttaagcagaa 2640
ctacgccctg agcctgcaga tcgagtacct gagcaagcag ctgcaggaga tcagcgacaa 2700
gctggacatc atcaacgtga acgtcctgat caacagcacc ctgaccgaga tcaccccggc 2760
ctaccagcgc atcaagtacg tgaacgagaa gttcgaagag ctgaccttcg ccaccgagac 2820
cagcagcaag gtgaagaagg acggcagccc ggccgacatc ctggacgagc tgaccgagct 2880
gaccgagctg gcgaagagcg tgaccaagaa cgacgtggac ggcttcgagt tctacctgaa 2940
caccttccac gacgtgatgg tgggcaacaa cctgttcggc cgcagcgccc tgaagaccgc 3000
cagcgagctg atcaccaagg agaacgtgaa gaccagcggc agcgaggtgg gcaacgtgta 3060
caacttcctg atcgtgctga ccgccctgca ggcccaggcc ttcctgaccc tgaccacctg 3120
tcgcaagctg ctgggcctgg ccgacatcga ctacaccagc atcatgaacg agcacttgaa 3180
caaggagaag gaggagttcc gcgtgaacat cctgccgacc ctgagcaaca ccttcagcaa 3240
cccgaactac gccaaggtga agggcagcga cgaggacgcc aagatgatcg tggaggctaa 3300
gccgggccac gcgttgatcg gcttcgagat cagcaacgac agcatcaccg tgctgaaggt 3360
gtacgaggcc aagctgaagc agaactacca ggtggacaag gacagcttga gcgaggtgat 3420
ctacggcgac atggacaagc tgctgtgtcc ggaccagagc gagcaaatct actacaccaa 3480
caacatcgtg ttcccgaacg agtacgtgat caccaagatc gacttcacca agaagatgaa 3540
gaccctgcgc tacgaggtga ccgccaactt ctacgacagc agcaccggcg agatcgacct 3600
gaacaagaag aaggtggaga gcagcgaggc cgagtaccgc accctgagcg cgaacgacga 3660
cggcgtctac atgccactgg gcgtgatcag cgagaccttc ctgaccccga tcaacggctt 3720
tggcctgcag gccgacgaga acagccgcct gatcaccctg acctgtaaga gctacctgcg 3780
cgagctgctg ctagccaccg acctgagcaa caaggagacc aagctgatcg tgccaccgag 3840
cggcttcatc agcaacatcg tggagaacgg cagcatcgag gaggacaacc tggagccgtg 3900
gaaggccaac aacaagaacg cctacgtcga ccacaccggc ggcgtgaacg gcaccaaggc 3960
cctgtacgtg cacaaggacg gcggcatcag ccagttcatc ggcgacaagc tgaagccgaa 4020
gaccgagtac gtgatccagt acaccgtgaa gggcaagcca tcgattcacc tgaaggacga 4080
gaacaccggc tacatccact acgaggacac caacaacaac ctggaggact accagaccat 4140
caacaagcgc ttcaccaccg gcaccgacct gaagggcgtg tacctgatcc tgaagagcca 4200
gaacggcgac gaggcctggg gcgacaactt catcatcctg gagatcagcc cgagcgagaa 4260
gctgctgagc ccggagctga tcaacaccaa caactggacc agcaccggca gcaccaacat 4320
cagcggcaac accctgaccc tgtaccaggg cggccgcggc atcctgaagc agaacctgca 4380
gctggacagc ttcagcacct accgcgtgta cttcagcgtg agcggcgacg ccaacgtgcg 4440
catccgcaac tcccgcgagg tgctgttcga gaagaggtac atgagcggcg ccaaggacgt 4500
gagcgagatg ttcaccacca agttcgagaa ggacaacttc tacatcgagc tgagccaggg 4560
caacaacctg tacggcggcc cgatcgtgca cttctacgac gtgagcatca agtaggagct 4620
ctgatgcaca gattgggatg agatattgga ggaaaacaaa gagggactga caattatctg 4680
cacaaagtgg agtagtcagt catcgatcag gaaccagaca ccagactttt attcatacag 4740
tgaagtgaag tgaagtgcag tgcagtgagt tgctggtttt tgtacaactt agtatgtatt 4800
tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccaggct 4860
acacggaccg cgatcgctta attaagcttg catgcctgca gtgcagcgtg acccggtcgt 4920
gcccctctct agagataatg agcattgcat gtctaagtta taaaaaatta ccacatattt 4980
tttttgtcac acttgtttga agtgcagttt atctatcttt atacatatat ttaaacttta 5040
ctctacgaat aatataatct atagtactac aataatatca gtgttttaga gaatcatata 5100
aatgaacagt tagacatggt ctaaaggaca attgagtatt ttgacaacag gactctacag 5160
ttttatcttt ttagtgtgca tgtgttctcc tttttttttg caaatagctt cacctatata 5220
atacttcatc cattttatta gtacatccat ttagggttta gggttaatgg tttttataga 5280
ctaatttttt tagtacatct attttattct attttagcct ctaaattaag aaaactaaaa 5340
ctctatttta gtttttttat ttaataattt agatataaaa tagaataaaa taaagtgact 5400
aaaaattaaa caaataccct ttaagaaatt aaaaaaacta aggaaacatt tttcttgttt 5460
cgagtagata atgccagcct gttaaacgcc gccgacgagt ctaacggaca ccaaccagcg 5520
aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca cggcatctct gtcgctgcct 5580
ctggacccct ctcgagagtt ccgctccacc gttggacttg ctccgctgtc ggcatccaga 5640
aattgcgtgg cggagcggca gacgtgagcc ggcacggcag gcggcctcct cctcctctca 5700
cggcaccggc agctacgggg gattcctttc ccaccgctcc ttcgctttcc cttcctcgcc 5760
cgccgtaata aatagacacc ccctccacac cctctttccc caacctcgtg ttgttcggag 5820
cgcacacaca cacaaccaga tctcccccaa atccacccgt cggcacctcc gcttcaaggt 5880
acgccgctcg tcctcccccc ccccccctct ctaccttctc tagatcggcg ttccggtcca 5940
tagttagggc ccggtagttc tacttctgtt catgtttgtg ttagatccgt gtttgtgtta 6000
gatccgtgct gttagcgttc gtacacggat gcgacctgta cgtcagacac gttctgattg 6060
ctaacttgcc agtgtttctc tttggggaat cctgggatgg ctctagccgt tccgcagacg 6120
ggatcgattt catgattttt tttgtttcgt tgcatagggt ttggtttgcc cttttccttt 6180
atttcaatat atgccgtgca cttgtttgtc gggtcatctt ttcatgcttt tttttgtctt 6240
ggttgtgatg atgtggtctg gttgggcggt cgttctagat cggagtagaa ttctgtttca 6300
aactacctgg tggatttatt aattttggat ctgtatgtgt gtgccataca tattcatagt 6360
tacgaattga agatgatgga tggaaatatc gatctaggat aggtatacat gttgatgcgg 6420
gttttactga tgcatataca gagatgcttt ttgttcgctt ggttgtgatg atgtggtgtg 6480
gttgggcggt cgttcattcg ttctagatcg gagtagaata ctgtttcaaa ctacctggtg 6540
tatttattaa ttttggaact gtatgtgtgt gtcatacatc ttcatagtta cgagtttaag 6600
atggatggaa atatcgatct aggataggta tacatgttga tgtgggtttt actgatgcat 6660
atacatgatg gcatatgcag catctattca tatgctctaa ccttgagtac ctatctatta 6720
taataaacaa gtatgtttta taattatttt gatcttgata tacttggatg atggcatatg 6780
cagcagctat atgtggattt ttttagccct gccttcatac gctatttatt tgcttggtac 6840
tgtttctttt gtcgatgctc accctgttgt ttggtgttac ttctgcaggg atctccgatc 6900
atgcaaaaac tcattaactc agtgcaaaac tatgcctggg gcagcaaaac ggcgttgact 6960
gaactttatg gtatggaaaa tccgtccagc cagccgatgg ccgagctgtg gatgggcgca 7020
catccgaaaa gcagttcacg agtgcagaat gccgccggag atatcgtttc actgcgtgat 7080
gtgattgaga gtgataaatc gactctgctc ggagaggccg ttgccaaacg ctttggcgaa 7140
ctgcctttcc tgttcaaagt attatgcgca gcacagccac tctccattca ggttcatcca 7200
aacaaacaca attctgaaat cggttttgcc aaagaaaatg ccgcaggtat cccgatggat 7260
gccgccgagc gtaactataa agatcctaac cacaagccgg agctggtttt tgcgctgacg 7320
cctttccttg cgatgaacgc gtttcgtgaa ttttccgaga ttgtctccct actccagccg 7380
gtcgcaggtg cacatccggc gattgctcac tttttacaac agcctgatgc cgaacgttta 7440
agcgaactgt tcgccagcct gttgaatatg cagggtgaag aaaaatcccg cgcgctggcg 7500
attttaaaat cggccctcga tagccagcag ggtgaaccgt ggcaaacgat tcgtttaatt 7560
tctgaatttt acccggaaga cagcggtctg ttctccccgc tattgctgaa tgtggtgaaa 7620
ttgaaccctg gcgaagcgat gttcctgttc gctgaaacac cgcacgctta cctgcaaggc 7680
gtggcgctgg aagtgatggc aaactccgat aacgtgctgc gtgcgggtct gacgcctaaa 7740
tacattgata ttccggaact ggttgccaat gtgaaattcg aagccaaacc ggctaaccag 7800
ttgttgaccc agccggtgaa acaaggtgca gaactggact tcccgattcc agtggatgat 7860
tttgccttct cgctgcatga ccttagtgat aaagaaacca ccattagcca gcagagtgcc 7920
gccattttgt tctgcgtcga aggcgatgca acgttgtgga aaggttctca gcagttacag 7980
cttaaaccgg gtgaatcagc gtttattgcc gccaacgaat caccggtgac tgtcaaaggc 8040
cacggccgtt tagcgcgtgt ttacaacaag ctgtaagagc ttactgaaaa aattaacatc 8100
tcttgctaag ctgggtcatg ggtcgtttaa gctgccgatg tgcctgcgtc gtctggtgcc 8160
ctctctccat atggaggttg tcaaagtatc tgctgttcgt gtcatgagtc gtgtcagtgt 8220
tggtttaata atggaccggt tgtgttgtgt gtgcgtacta cccagaacta tgacaaatca 8280
tgaataagtt tgatgtttga aattaaagcc tgtgctcatt atgttctgtc tttcagttgt 8340
ctcctaatat ttgcctccag gtactggcta tctaccgttt cttacttagg aggtgtttga 8400
atgcactaaa actaatagtt agtggctaaa attagttaaa acatccaaac accatagcta 8460
atagttgaac tattagctat ttttggaaaa ttagttaata gtgaggtagt tatttgttag 8520
ctagctaatt caactaacaa tttttagcca actaacaatt agtttcagtg cattcaaaca 8580
cccccttaat gttaacgtgg ttctatctac cgtctcctaa tatatggttg attgttcggt 8640
ttgttgctat gctattgggt tctgattgct gctagttctt gctgaatcca gaagttctcg 8700
tagtatagct cagattcata ttatttattt gagtgataag tgatccaggt tattactatg 8760
ttagctaggt tttttttaca aggataaatt atctgtgatc ataattctta tgaaagcttt 8820
atgtttcctg gaggcagtgg catgcaatgc atgacagcaa cttgatcaca ccagctgagg 8880
tagatacggt aacaaggttc ttaaatctgt tcaccaaatc attggagaac acacatacac 8940
attcttgcca gtcttggtta gagaaatttc atgacaaaat gccaaagctg tcttgactct 9000
tcacttttgg ccatgagtcg tgacttagtt tggtttaatg gaccggttct cctagcttgt 9060
tctactcaaa actgttgttg atgcgaataa gttgtgatgg ttgatctctg gattttgttt 9120
tgctctcaat agtggacgag attagatagc ctgcaggccc gggggcgcgc cctaattagc 9180
taacggccag gatcgccgcg tgagccttta gcaactagct agattaatta acgcaatctg 9240
ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc 9300
caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca 9360
gaattaattc tcatgtttga cagcttatca tcgactgcac ggtgcaccaa tgcttctggc 9420
gtcaggcagc catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac tgcataattc 9480
gtgtcgctca aggcgcactc ccgttctgga taatgttttt tgcgccgaca tcataacggt 9540
tctggcaaat attctgaaat gagctgttga caattaatca tccggctcgt ataatgtgtg 9600
gaattgtgag cggataacaa tttcacacag gaaacagacc atgagggaag cgttgatcgc 9660
cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac 9720
gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga 9780
tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat 9840
caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga 9900
agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact 9960
gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat 10020
cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg 10080
tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa 10140
tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt 10200
gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt 10260
cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc 10320
taggcaggct tatcttggac aagaagatcg cttggcctcg cgcgcagatc agttggaaga 10380
atttgttcac tacgtgaaag gcgagatcac caaagtagtc ggcaaataaa gctctagtgg 10440
atctccgtac ccagggatct ggctcgcggc ggacgcacga cgccggggcg agaccatagg 10500
cgatctccta aatcaatagt agctgtaacc tcgaagcgtt tcacttgtaa caacgattga 10560
gaatttttgt cataaaattg aaatacttgg ttcgcatttt tgtcatccgc ggtcagccgc 10620
aattctgacg aactgcccat ttagctggag atgattgtac atccttcacg tgaaaatttc 10680
tcaagcgctg tgaacaaggg ttcagatttt agattgaaag gtgagccgtt gaaacacgtt 10740
cttcttgtcg atgacgacgt cgctatgcgg catcttatta ttgaatacct tacgatccac 10800
gccttcaaag tgaccgcggt agccgacagc acccagttca caagagtact ctcttccgcg 10860
acggtcgatg tcgtggttgt tgatctagat ttaggtcgtg aagatgggct cgagatcgtt 10920
cgtaatctgg cggcaaagtc tgatattcca atcataatta tcagtggcga ccgccttgag 10980
gagacggata aagttgttgc actcgagcta ggagcaagtg attttatcgc taagccgttc 11040
agtatcagag agtttctagc acgcattcgg gttgccttgc gcgtgcgccc caacgttgtc 11100
cgctccaaag accgacggtc tttttgtttt actgactgga cacttaatct caggcaacgt 11160
cgcttgatgt ccgaagctgg cggtgaggtg aaacttacgg caggtgagtt caatcttctc 11220
ctcgcgtttt tagagaaacc ccgcgacgtt ctatcgcgcg agcaacttct cattgccagt 11280
cgagtacgcg acgaggaggt ttatgacagg agtatagatg ttctcatttt gaggctgcgc 11340
cgcaaacttg aggcagatcc gtcaagccct caactgataa aaacagcaag aggtgccggt 11400
tatttctttg acgcggacgt gcaggtttcg cacgggggga cgatggcagc ctgagccaat 11460
tcccagatcc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg 11520
gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc 11580
aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc 11640
gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg 11700
gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc 11760
gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg 11820
tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg 11880
ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga 11940
accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg 12000
acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa 12060
cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg 12120
gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga 12180
gcgaaaccgg gcggccggag tacatcgaga tcgagctggc tgattggatg taccgcgaga 12240
tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc 12300
ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca 12360
gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct 12420
gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg 12480
aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag 12540
catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa 12600
aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca 12660
ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca 12720
tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac 12780
ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg 12840
aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc 12900
gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac 12960
cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgctgaggt ctgcctcgtg 13020
aagaaggtgt tgctgactca taccaggcct gaatcgcccc atcatccagc cagaaagtga 13080
gggagccacg gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt 13140
gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag 13200
caaaagttcg atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa tgctctgcca 13260
gtgttacaac caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg 13320
caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga 13380
aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat 13440
tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc 13500
aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gctctgcatt 13560
aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 13620
cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 13680
aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 13740
aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 13800
tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 13860
caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 13920
cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 13980
ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 14040
gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 14100
agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 14160
gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 14220
acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 14280
gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 14340
gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 14400
cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 14460
caaaaaggat cttcacctag atccttttga tccggaatta 14500
<210> 106
<211> 14499
<212> DNA
<213> 人工序列
<220>
<223> 23711载体
<400> 106
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta 1260
gatcggcgtt ccggtccata gttagggccc ggtagttcta cttctgttca tgtttgtgtt 1320
agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg 1380
tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct 1440
ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt 1500
ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt 1560
catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg 1620
gagtagaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt 1680
gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag 1740
gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg 1800
ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact 1860
gtttcaaact acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt 1920
catagttacg agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg 1980
tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc 2040
ttgagtacct atctattata ataaacaagt atgttttata attattttga tcttgatata 2100
cttggatgat ggcatatcca gcagctatat gtggattttt ttagccctgc cttcatacgc 2160
tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt 2220
ctgcaggatc ctctaagtag cagccatgaa caagaacaac accaagctga gcacccgcgc 2280
cctgccgagc ttcatcgact acttcaacgg catctacggc ttcgccaccg gcatcaagga 2340
catcatgaac atgatcttca agaccgacac cggcggcgac ctgaccctgg acgagatcct 2400
gaagaaccag cagctgctga acgacatcag cggcaagctg gacggcgtga acggcagcct 2460
gaacgacctg atcgcccagg gcaacctgaa caccgagctg agcaaggaga tccttaagat 2520
cgccaacgag cagaaccagg tgctgaacga cgtgaacaac aagctggacg ccatcaacac 2580
catgctgcgc gtgtacctgc cgaagatcac cagcatgctg agcgacgtga ttaagcagaa 2640
ctacgccctg agcctgcaga tcgagtacct gagcaagcag ctgcaggaga tcagcgacaa 2700
gctggacatc atcaacgtga acgtcctgat caacagcacc ctgaccgaga tcaccccggc 2760
ctaccagcgc atcaagtacg tgaacgagaa gttcgaagag ctgaccttcg ccaccgagac 2820
cagcagcaag gtgaagaagg acggcagccc ggccgacatc ctggacgagc tgaccgagct 2880
gaccgagctg gcgaagagcg tgaccaagaa cgacgtggac ggcttcgagt tctacctgaa 2940
caccttccac gacgtgatgg tgggcaacaa cctgttcggc cgcagcgccc tgaagaccgc 3000
cagcgagctg atcaccaagg agaacgtgaa gaccagcggc agcgaggtgg gcaacgtgta 3060
caacttcctg atcgtgctga ccgccctgca ggcccaggcc ttcctgaccc tgaccacctg 3120
tcgcaagctg ctgggcctgg ccgacatcga ctacaccagc atcatgaacg agcacttgaa 3180
caaggagaag gaggagttcc gcgtgaacat cctgccgacc ctgagcaaca ccttcagcaa 3240
cccgaactac gccaaggtga agggcagcga cgaggacgcc aagatgatcg tggaggctaa 3300
gccgggccac gcgttgatcg gcttcgagat cagcaacgac agcatcaccg tgctgaaggt 3360
gtacgaggcc aagctgaagc agaactacca ggtggacaag gacagcttga gcgaggtgat 3420
ctacggcgac atggacaagc tgctgtgtcc ggaccagagc gagcaaatct actacaccaa 3480
caacatcgtg ttcccgaacg agtacgtgat caccaagatc gacttcacca agaagatgaa 3540
gaccctgcgc tacgaggtga ccgccaactt ctacgacagc agcaccggcg agatcgacct 3600
gaacaagaag aaggtggaga gcagcgaggc cgagtaccgc accctgagcg cgaacgacga 3660
cggcgtctac atgccactgg gcgtgatcag cgagaccttc ctgaccccga tcaacggctt 3720
tggcctgcag gccgacgaga acagccgcct gatcaccctg acctgtaaga gctacctgcg 3780
cgagctgctg ctagccaccg acctgagcaa caaggagacc aagctgatcg tgccaccgag 3840
cggcttcatc agcaacatcg tggagaacgg cagcatcgag gaggacaacc tggagccgtg 3900
gaaggccaac aacaagaacg cctacgtcga ccacaccggc ggcgtgaacg gcaccaaggc 3960
cctgtacgtg cacaaggacg gcggcatcag ccagttcatc ggcgacaagc tgaagccgaa 4020
gaccgagtac gtgatccagt acaccgtgaa gggcaagcca tcgattcacc tgaaggacga 4080
gaacaccggc tacatccact acgaggacac caacaacaac ctggaggact accagaccat 4140
caacaagcgc ttcaccaccg gcaccgacct gaagggcgtg tacctgatcc tgaagagcca 4200
gaacggcgac gaggcctggg gcgacaactt catcatcctg gagatcagcc cgagcgagaa 4260
gctgctgagc ccggagctga tcaacaccaa caactggacc agcaccggca gcaccaacat 4320
cagcggcaac accctgaccc tgtaccaggg cggccgcggc atcctgaagc agaacctgca 4380
gctggacagc ttcagcacct accgcgtgta cttcagcgtg agcggcgacg ccaacgtgcg 4440
catccgcaac tcccgcgagg tgctgttcga gaagaggtac atgagcggcg ccaaggacgt 4500
gagcgagatg ttcaccacca agttcgagaa ggacaacttc tacatcgagc tgagccaggg 4560
caacaacctg tacggcggcc cgatcgtgca cttctacgac gtgagcatca agtaggagct 4620
ctgtccctca ctacgtccgg ttcagttgcc tctaatccta tccctcgcca cgttatctgc 4680
acaaagtgga gtagtcagtc atcgatcagg aaccagacac cagactttta ttcatacagt 4740
gaagtgaagt gaagtgcagt gcagtgagtt gctggttttt gtacaactta gtatgtattt 4800
gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccaggcta 4860
cacggaccgc gatcgcttaa ttaagcttgc atgcctgcag tgcagcgtga cccggtcgtg 4920
cccctctcta gagataatga gcattgcatg tctaagttat aaaaaattac cacatatttt 4980
ttttgtcaca cttgtttgaa gtgcagttta tctatcttta tacatatatt taaactttac 5040
tctacgaata atataatcta tagtactaca ataatatcag tgttttagag aatcatataa 5100
atgaacagtt agacatggtc taaaggacaa ttgagtattt tgacaacagg actctacagt 5160
tttatctttt tagtgtgcat gtgttctcct ttttttttgc aaatagcttc acctatataa 5220
tacttcatcc attttattag tacatccatt tagggtttag ggttaatggt ttttatagac 5280
taattttttt agtacatcta ttttattcta ttttagcctc taaattaaga aaactaaaac 5340
tctattttag tttttttatt taataattta gatataaaat agaataaaat aaagtgacta 5400
aaaattaaac aaataccctt taagaaatta aaaaaactaa ggaaacattt ttcttgtttc 5460
gagtagataa tgccagcctg ttaaacgccg ccgacgagtc taacggacac caaccagcga 5520
accagcagcg tcgcgtcggg ccaagcgaag cagacggcac ggcatctctg tcgctgcctc 5580
tggacccctc tcgagagttc cgctccaccg ttggacttgc tccgctgtcg gcatccagaa 5640
attgcgtggc ggagcggcag acgtgagccg gcacggcagg cggcctcctc ctcctctcac 5700
ggcaccggca gctacggggg attcctttcc caccgctcct tcgctttccc ttcctcgccc 5760
gccgtaataa atagacaccc cctccacacc ctctttcccc aacctcgtgt tgttcggagc 5820
gcacacacac acaaccagat ctcccccaaa tccacccgtc ggcacctccg cttcaaggta 5880
cgccgctcgt cctccccccc cccccctctc taccttctct agatcggcgt tccggtccat 5940
agttagggcc cggtagttct acttctgttc atgtttgtgt tagatccgtg tttgtgttag 6000
atccgtgctg ttagcgttcg tacacggatg cgacctgtac gtcagacacg ttctgattgc 6060
taacttgcca gtgtttctct ttggggaatc ctgggatggc tctagccgtt ccgcagacgg 6120
gatcgatttc atgatttttt ttgtttcgtt gcatagggtt tggtttgccc ttttccttta 6180
tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg 6240
gttgtgatga tgtggtctgg ttgggcggtc gttctagatc ggagtagaat tctgtttcaa 6300
actacctggt ggatttatta attttggatc tgtatgtgtg tgccatacat attcatagtt 6360
acgaattgaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg 6420
ttttactgat gcatatacag agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg 6480
ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgt 6540
atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac gagtttaaga 6600
tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata 6660
tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc tatctattat 6720
aataaacaag tatgttttat aattattttg atcttgatat acttggatga tggcatatgc 6780
agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt gcttggtact 6840
gtttcttttg tcgatgctca ccctgttgtt tggtgttact tctgcaggga tctccgatca 6900
tgcaaaaact cattaactca gtgcaaaact atgcctgggg cagcaaaacg gcgttgactg 6960
aactttatgg tatggaaaat ccgtccagcc agccgatggc cgagctgtgg atgggcgcac 7020
atccgaaaag cagttcacga gtgcagaatg ccgccggaga tatcgtttca ctgcgtgatg 7080
tgattgagag tgataaatcg actctgctcg gagaggccgt tgccaaacgc tttggcgaac 7140
tgcctttcct gttcaaagta ttatgcgcag cacagccact ctccattcag gttcatccaa 7200
acaaacacaa ttctgaaatc ggttttgcca aagaaaatgc cgcaggtatc ccgatggatg 7260
ccgccgagcg taactataaa gatcctaacc acaagccgga gctggttttt gcgctgacgc 7320
ctttccttgc gatgaacgcg tttcgtgaat tttccgagat tgtctcccta ctccagccgg 7380
tcgcaggtgc acatccggcg attgctcact ttttacaaca gcctgatgcc gaacgtttaa 7440
gcgaactgtt cgccagcctg ttgaatatgc agggtgaaga aaaatcccgc gcgctggcga 7500
ttttaaaatc ggccctcgat agccagcagg gtgaaccgtg gcaaacgatt cgtttaattt 7560
ctgaatttta cccggaagac agcggtctgt tctccccgct attgctgaat gtggtgaaat 7620
tgaaccctgg cgaagcgatg ttcctgttcg ctgaaacacc gcacgcttac ctgcaaggcg 7680
tggcgctgga agtgatggca aactccgata acgtgctgcg tgcgggtctg acgcctaaat 7740
acattgatat tccggaactg gttgccaatg tgaaattcga agccaaaccg gctaaccagt 7800
tgttgaccca gccggtgaaa caaggtgcag aactggactt cccgattcca gtggatgatt 7860
ttgccttctc gctgcatgac cttagtgata aagaaaccac cattagccag cagagtgccg 7920
ccattttgtt ctgcgtcgaa ggcgatgcaa cgttgtggaa aggttctcag cagttacagc 7980
ttaaaccggg tgaatcagcg tttattgccg ccaacgaatc accggtgact gtcaaaggcc 8040
acggccgttt agcgcgtgtt tacaacaagc tgtaagagct tactgaaaaa attaacatct 8100
cttgctaagc tgggtcatgg gtcgtttaag ctgccgatgt gcctgcgtcg tctggtgccc 8160
tctctccata tggaggttgt caaagtatct gctgttcgtg tcatgagtcg tgtcagtgtt 8220
ggtttaataa tggaccggtt gtgttgtgtg tgcgtactac ccagaactat gacaaatcat 8280
gaataagttt gatgtttgaa attaaagcct gtgctcatta tgttctgtct ttcagttgtc 8340
tcctaatatt tgcctccagg tactggctat ctaccgtttc ttacttagga ggtgtttgaa 8400
tgcactaaaa ctaatagtta gtggctaaaa ttagttaaaa catccaaaca ccatagctaa 8460
tagttgaact attagctatt tttggaaaat tagttaatag tgaggtagtt atttgttagc 8520
tagctaattc aactaacaat ttttagccaa ctaacaatta gtttcagtgc attcaaacac 8580
ccccttaatg ttaacgtggt tctatctacc gtctcctaat atatggttga ttgttcggtt 8640
tgttgctatg ctattgggtt ctgattgctg ctagttcttg ctgaatccag aagttctcgt 8700
agtatagctc agattcatat tatttatttg agtgataagt gatccaggtt attactatgt 8760
tagctaggtt ttttttacaa ggataaatta tctgtgatca taattcttat gaaagcttta 8820
tgtttcctgg aggcagtggc atgcaatgca tgacagcaac ttgatcacac cagctgaggt 8880
agatacggta acaaggttct taaatctgtt caccaaatca ttggagaaca cacatacaca 8940
ttcttgccag tcttggttag agaaatttca tgacaaaatg ccaaagctgt cttgactctt 9000
cacttttggc catgagtcgt gacttagttt ggtttaatgg accggttctc ctagcttgtt 9060
ctactcaaaa ctgttgttga tgcgaataag ttgtgatggt tgatctctgg attttgtttt 9120
gctctcaata gtggacgaga ttagatagcc tgcaggcccg ggggcgcgcc ctaattagct 9180
aacggccagg atcgccgcgt gagcctttag caactagcta gattaattaa cgcaatctgt 9240
tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc 9300
aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag 9360
aattaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg 9420
tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg 9480
tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt 9540
ctggcaaata ttctgaaatg agctgttgac aattaatcat ccggctcgta taatgtgtgg 9600
aattgtgagc ggataacaat ttcacacagg aaacagacca tgagggaagc gttgatcgcc 9660
gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg 9720
ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat 9780
attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc 9840
aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa 9900
gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg 9960
caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc 10020
gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt 10080
ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat 10140
gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg 10200
cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc 10260
gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct 10320
aggcaggctt atcttggaca agaagatcgc ttggcctcgc gcgcagatca gttggaagaa 10380
tttgttcact acgtgaaagg cgagatcacc aaagtagtcg gcaaataaag ctctagtgga 10440
tctccgtacc cagggatctg gctcgcggcg gacgcacgac gccggggcga gaccataggc 10500
gatctcctaa atcaatagta gctgtaacct cgaagcgttt cacttgtaac aacgattgag 10560
aatttttgtc ataaaattga aatacttggt tcgcattttt gtcatccgcg gtcagccgca 10620
attctgacga actgcccatt tagctggaga tgattgtaca tccttcacgt gaaaatttct 10680
caagcgctgt gaacaagggt tcagatttta gattgaaagg tgagccgttg aaacacgttc 10740
ttcttgtcga tgacgacgtc gctatgcggc atcttattat tgaatacctt acgatccacg 10800
ccttcaaagt gaccgcggta gccgacagca cccagttcac aagagtactc tcttccgcga 10860
cggtcgatgt cgtggttgtt gatctagatt taggtcgtga agatgggctc gagatcgttc 10920
gtaatctggc ggcaaagtct gatattccaa tcataattat cagtggcgac cgccttgagg 10980
agacggataa agttgttgca ctcgagctag gagcaagtga ttttatcgct aagccgttca 11040
gtatcagaga gtttctagca cgcattcggg ttgccttgcg cgtgcgcccc aacgttgtcc 11100
gctccaaaga ccgacggtct ttttgtttta ctgactggac acttaatctc aggcaacgtc 11160
gcttgatgtc cgaagctggc ggtgaggtga aacttacggc aggtgagttc aatcttctcc 11220
tcgcgttttt agagaaaccc cgcgacgttc tatcgcgcga gcaacttctc attgccagtc 11280
gagtacgcga cgaggaggtt tatgacagga gtatagatgt tctcattttg aggctgcgcc 11340
gcaaacttga ggcagatccg tcaagccctc aactgataaa aacagcaaga ggtgccggtt 11400
atttctttga cgcggacgtg caggtttcgc acggggggac gatggcagcc tgagccaatt 11460
cccagatccc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg 11520
cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca 11580
acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg 11640
caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg 11700
cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg 11760
cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt 11820
gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc 11880
cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa 11940
ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga 12000
cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac 12060
ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg 12120
ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag 12180
cgaaaccggg cggccggagt acatcgagat cgagctggct gattggatgt accgcgagat 12240
cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc 12300
cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag 12360
atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg 12420
tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga 12480
ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc 12540
atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa 12600
aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat 12660
tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat 12720
gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact 12780
tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga 12840
agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg 12900
tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc 12960
agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgctgaggtc tgcctcgtga 13020
agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc agaaagtgag 13080
ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt tgaacttttg 13140
ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct tcaactcagc 13200
aaaagttcga tttattcaac aaagccgccg tcccgtcaag tcagcgtaat gctctgccag 13260
tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc 13320
aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa 13380
ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt 13440
ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca 13500
agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag ctctgcatta 13560
atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 13620
gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 13680
ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 13740
aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 13800
ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 13860
aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 13920
gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 13980
tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 14040
tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 14100
gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 14160
cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 14220
cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 14280
agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 14340
caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 14400
ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 14460
aaaaaggatc ttcacctaga tccttttgat ccggaatta 14499
<210> 107
<211> 14500
<212> DNA
<213> 人工序列
<220>
<223> 23712载体
<400> 107
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta 1260
gatcggcgtt ccggtccata gttagggccc ggtagttcta cttctgttca tgtttgtgtt 1320
agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg 1380
tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct 1440
ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt 1500
ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt 1560
catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg 1620
gagtagaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt 1680
gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag 1740
gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg 1800
ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact 1860
gtttcaaact acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt 1920
catagttacg agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg 1980
tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc 2040
ttgagtacct atctattata ataaacaagt atgttttata attattttga tcttgatata 2100
cttggatgat ggcatatcca gcagctatat gtggattttt ttagccctgc cttcatacgc 2160
tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt 2220
ctgcaggatc ctctaagtag cagccatgaa caagaacaac accaagctga gcacccgcgc 2280
cctgccgagc ttcatcgact acttcaacgg catctacggc ttcgccaccg gcatcaagga 2340
catcatgaac atgatcttca agaccgacac cggcggcgac ctgaccctgg acgagatcct 2400
gaagaaccag cagctgctga acgacatcag cggcaagctg gacggcgtga acggcagcct 2460
gaacgacctg atcgcccagg gcaacctgaa caccgagctg agcaaggaga tccttaagat 2520
cgccaacgag cagaaccagg tgctgaacga cgtgaacaac aagctggacg ccatcaacac 2580
catgctgcgc gtgtacctgc cgaagatcac cagcatgctg agcgacgtga ttaagcagaa 2640
ctacgccctg agcctgcaga tcgagtacct gagcaagcag ctgcaggaga tcagcgacaa 2700
gctggacatc atcaacgtga acgtcctgat caacagcacc ctgaccgaga tcaccccggc 2760
ctaccagcgc atcaagtacg tgaacgagaa gttcgaagag ctgaccttcg ccaccgagac 2820
cagcagcaag gtgaagaagg acggcagccc ggccgacatc ctggacgagc tgaccgagct 2880
gaccgagctg gcgaagagcg tgaccaagaa cgacgtggac ggcttcgagt tctacctgaa 2940
caccttccac gacgtgatgg tgggcaacaa cctgttcggc cgcagcgccc tgaagaccgc 3000
cagcgagctg atcaccaagg agaacgtgaa gaccagcggc agcgaggtgg gcaacgtgta 3060
caacttcctg atcgtgctga ccgccctgca ggcccaggcc ttcctgaccc tgaccacctg 3120
tcgcaagctg ctgggcctgg ccgacatcga ctacaccagc atcatgaacg agcacttgaa 3180
caaggagaag gaggagttcc gcgtgaacat cctgccgacc ctgagcaaca ccttcagcaa 3240
cccgaactac gccaaggtga agggcagcga cgaggacgcc aagatgatcg tggaggctaa 3300
gccgggccac gcgttgatcg gcttcgagat cagcaacgac agcatcaccg tgctgaaggt 3360
gtacgaggcc aagctgaagc agaactacca ggtggacaag gacagcttga gcgaggtgat 3420
ctacggcgac atggacaagc tgctgtgtcc ggaccagagc gagcaaatct actacaccaa 3480
caacatcgtg ttcccgaacg agtacgtgat caccaagatc gacttcacca agaagatgaa 3540
gaccctgcgc tacgaggtga ccgccaactt ctacgacagc agcaccggcg agatcgacct 3600
gaacaagaag aaggtggaga gcagcgaggc cgagtaccgc accctgagcg cgaacgacga 3660
cggcgtctac atgccactgg gcgtgatcag cgagaccttc ctgaccccga tcaacggctt 3720
tggcctgcag gccgacgaga acagccgcct gatcaccctg acctgtaaga gctacctgcg 3780
cgagctgctg ctagccaccg acctgagcaa caaggagacc aagctgatcg tgccaccgag 3840
cggcttcatc agcaacatcg tggagaacgg cagcatcgag gaggacaacc tggagccgtg 3900
gaaggccaac aacaagaacg cctacgtcga ccacaccggc ggcgtgaacg gcaccaaggc 3960
cctgtacgtg cacaaggacg gcggcatcag ccagttcatc ggcgacaagc tgaagccgaa 4020
gaccgagtac gtgatccagt acaccgtgaa gggcaagcca tcgattcacc tgaaggacga 4080
gaacaccggc tacatccact acgaggacac caacaacaac ctggaggact accagaccat 4140
caacaagcgc ttcaccaccg gcaccgacct gaagggcgtg tacctgatcc tgaagagcca 4200
gaacggcgac gaggcctggg gcgacaactt catcatcctg gagatcagcc cgagcgagaa 4260
gctgctgagc ccggagctga tcaacaccaa caactggacc agcaccggca gcaccaacat 4320
cagcggcaac accctgaccc tgtaccaggg cggccgcggc atcctgaagc agaacctgca 4380
gctggacagc ttcagcacct accgcgtgta cttcagcgtg agcggcgacg ccaacgtgcg 4440
catccgcaac tcccgcgagg tgctgttcga gaagaggtac atgagcggcg ccaaggacgt 4500
gagcgagatg ttcaccacca agttcgagaa ggacaacttc tacatcgagc tgagccaggg 4560
caacaacctg tacggcggcc cgatcgtgca cttctacgac gtgagcatca agtaggagct 4620
ctatacgcac acacatgttg agatattaga ggaaactgaa gaattacgcc acattatctg 4680
cacaaagtgg agtagtcagt catcgatcag gaaccagaca ccagactttt attcatacag 4740
tgaagtgaag tgaagtgcag tgcagtgagt tgctggtttt tgtacaactt agtatgtatt 4800
tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccaggct 4860
acacggaccg cgatcgctta attaagcttg catgcctgca gtgcagcgtg acccggtcgt 4920
gcccctctct agagataatg agcattgcat gtctaagtta taaaaaatta ccacatattt 4980
tttttgtcac acttgtttga agtgcagttt atctatcttt atacatatat ttaaacttta 5040
ctctacgaat aatataatct atagtactac aataatatca gtgttttaga gaatcatata 5100
aatgaacagt tagacatggt ctaaaggaca attgagtatt ttgacaacag gactctacag 5160
ttttatcttt ttagtgtgca tgtgttctcc tttttttttg caaatagctt cacctatata 5220
atacttcatc cattttatta gtacatccat ttagggttta gggttaatgg tttttataga 5280
ctaatttttt tagtacatct attttattct attttagcct ctaaattaag aaaactaaaa 5340
ctctatttta gtttttttat ttaataattt agatataaaa tagaataaaa taaagtgact 5400
aaaaattaaa caaataccct ttaagaaatt aaaaaaacta aggaaacatt tttcttgttt 5460
cgagtagata atgccagcct gttaaacgcc gccgacgagt ctaacggaca ccaaccagcg 5520
aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca cggcatctct gtcgctgcct 5580
ctggacccct ctcgagagtt ccgctccacc gttggacttg ctccgctgtc ggcatccaga 5640
aattgcgtgg cggagcggca gacgtgagcc ggcacggcag gcggcctcct cctcctctca 5700
cggcaccggc agctacgggg gattcctttc ccaccgctcc ttcgctttcc cttcctcgcc 5760
cgccgtaata aatagacacc ccctccacac cctctttccc caacctcgtg ttgttcggag 5820
cgcacacaca cacaaccaga tctcccccaa atccacccgt cggcacctcc gcttcaaggt 5880
acgccgctcg tcctcccccc ccccccctct ctaccttctc tagatcggcg ttccggtcca 5940
tagttagggc ccggtagttc tacttctgtt catgtttgtg ttagatccgt gtttgtgtta 6000
gatccgtgct gttagcgttc gtacacggat gcgacctgta cgtcagacac gttctgattg 6060
ctaacttgcc agtgtttctc tttggggaat cctgggatgg ctctagccgt tccgcagacg 6120
ggatcgattt catgattttt tttgtttcgt tgcatagggt ttggtttgcc cttttccttt 6180
atttcaatat atgccgtgca cttgtttgtc gggtcatctt ttcatgcttt tttttgtctt 6240
ggttgtgatg atgtggtctg gttgggcggt cgttctagat cggagtagaa ttctgtttca 6300
aactacctgg tggatttatt aattttggat ctgtatgtgt gtgccataca tattcatagt 6360
tacgaattga agatgatgga tggaaatatc gatctaggat aggtatacat gttgatgcgg 6420
gttttactga tgcatataca gagatgcttt ttgttcgctt ggttgtgatg atgtggtgtg 6480
gttgggcggt cgttcattcg ttctagatcg gagtagaata ctgtttcaaa ctacctggtg 6540
tatttattaa ttttggaact gtatgtgtgt gtcatacatc ttcatagtta cgagtttaag 6600
atggatggaa atatcgatct aggataggta tacatgttga tgtgggtttt actgatgcat 6660
atacatgatg gcatatgcag catctattca tatgctctaa ccttgagtac ctatctatta 6720
taataaacaa gtatgtttta taattatttt gatcttgata tacttggatg atggcatatg 6780
cagcagctat atgtggattt ttttagccct gccttcatac gctatttatt tgcttggtac 6840
tgtttctttt gtcgatgctc accctgttgt ttggtgttac ttctgcaggg atctccgatc 6900
atgcaaaaac tcattaactc agtgcaaaac tatgcctggg gcagcaaaac ggcgttgact 6960
gaactttatg gtatggaaaa tccgtccagc cagccgatgg ccgagctgtg gatgggcgca 7020
catccgaaaa gcagttcacg agtgcagaat gccgccggag atatcgtttc actgcgtgat 7080
gtgattgaga gtgataaatc gactctgctc ggagaggccg ttgccaaacg ctttggcgaa 7140
ctgcctttcc tgttcaaagt attatgcgca gcacagccac tctccattca ggttcatcca 7200
aacaaacaca attctgaaat cggttttgcc aaagaaaatg ccgcaggtat cccgatggat 7260
gccgccgagc gtaactataa agatcctaac cacaagccgg agctggtttt tgcgctgacg 7320
cctttccttg cgatgaacgc gtttcgtgaa ttttccgaga ttgtctccct actccagccg 7380
gtcgcaggtg cacatccggc gattgctcac tttttacaac agcctgatgc cgaacgttta 7440
agcgaactgt tcgccagcct gttgaatatg cagggtgaag aaaaatcccg cgcgctggcg 7500
attttaaaat cggccctcga tagccagcag ggtgaaccgt ggcaaacgat tcgtttaatt 7560
tctgaatttt acccggaaga cagcggtctg ttctccccgc tattgctgaa tgtggtgaaa 7620
ttgaaccctg gcgaagcgat gttcctgttc gctgaaacac cgcacgctta cctgcaaggc 7680
gtggcgctgg aagtgatggc aaactccgat aacgtgctgc gtgcgggtct gacgcctaaa 7740
tacattgata ttccggaact ggttgccaat gtgaaattcg aagccaaacc ggctaaccag 7800
ttgttgaccc agccggtgaa acaaggtgca gaactggact tcccgattcc agtggatgat 7860
tttgccttct cgctgcatga ccttagtgat aaagaaacca ccattagcca gcagagtgcc 7920
gccattttgt tctgcgtcga aggcgatgca acgttgtgga aaggttctca gcagttacag 7980
cttaaaccgg gtgaatcagc gtttattgcc gccaacgaat caccggtgac tgtcaaaggc 8040
cacggccgtt tagcgcgtgt ttacaacaag ctgtaagagc ttactgaaaa aattaacatc 8100
tcttgctaag ctgggtcatg ggtcgtttaa gctgccgatg tgcctgcgtc gtctggtgcc 8160
ctctctccat atggaggttg tcaaagtatc tgctgttcgt gtcatgagtc gtgtcagtgt 8220
tggtttaata atggaccggt tgtgttgtgt gtgcgtacta cccagaacta tgacaaatca 8280
tgaataagtt tgatgtttga aattaaagcc tgtgctcatt atgttctgtc tttcagttgt 8340
ctcctaatat ttgcctccag gtactggcta tctaccgttt cttacttagg aggtgtttga 8400
atgcactaaa actaatagtt agtggctaaa attagttaaa acatccaaac accatagcta 8460
atagttgaac tattagctat ttttggaaaa ttagttaata gtgaggtagt tatttgttag 8520
ctagctaatt caactaacaa tttttagcca actaacaatt agtttcagtg cattcaaaca 8580
cccccttaat gttaacgtgg ttctatctac cgtctcctaa tatatggttg attgttcggt 8640
ttgttgctat gctattgggt tctgattgct gctagttctt gctgaatcca gaagttctcg 8700
tagtatagct cagattcata ttatttattt gagtgataag tgatccaggt tattactatg 8760
ttagctaggt tttttttaca aggataaatt atctgtgatc ataattctta tgaaagcttt 8820
atgtttcctg gaggcagtgg catgcaatgc atgacagcaa cttgatcaca ccagctgagg 8880
tagatacggt aacaaggttc ttaaatctgt tcaccaaatc attggagaac acacatacac 8940
attcttgcca gtcttggtta gagaaatttc atgacaaaat gccaaagctg tcttgactct 9000
tcacttttgg ccatgagtcg tgacttagtt tggtttaatg gaccggttct cctagcttgt 9060
tctactcaaa actgttgttg atgcgaataa gttgtgatgg ttgatctctg gattttgttt 9120
tgctctcaat agtggacgag attagatagc ctgcaggccc gggggcgcgc cctaattagc 9180
taacggccag gatcgccgcg tgagccttta gcaactagct agattaatta acgcaatctg 9240
ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc 9300
caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca 9360
gaattaattc tcatgtttga cagcttatca tcgactgcac ggtgcaccaa tgcttctggc 9420
gtcaggcagc catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac tgcataattc 9480
gtgtcgctca aggcgcactc ccgttctgga taatgttttt tgcgccgaca tcataacggt 9540
tctggcaaat attctgaaat gagctgttga caattaatca tccggctcgt ataatgtgtg 9600
gaattgtgag cggataacaa tttcacacag gaaacagacc atgagggaag cgttgatcgc 9660
cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac 9720
gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga 9780
tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat 9840
caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga 9900
agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact 9960
gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat 10020
cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg 10080
tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa 10140
tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt 10200
gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt 10260
cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc 10320
taggcaggct tatcttggac aagaagatcg cttggcctcg cgcgcagatc agttggaaga 10380
atttgttcac tacgtgaaag gcgagatcac caaagtagtc ggcaaataaa gctctagtgg 10440
atctccgtac ccagggatct ggctcgcggc ggacgcacga cgccggggcg agaccatagg 10500
cgatctccta aatcaatagt agctgtaacc tcgaagcgtt tcacttgtaa caacgattga 10560
gaatttttgt cataaaattg aaatacttgg ttcgcatttt tgtcatccgc ggtcagccgc 10620
aattctgacg aactgcccat ttagctggag atgattgtac atccttcacg tgaaaatttc 10680
tcaagcgctg tgaacaaggg ttcagatttt agattgaaag gtgagccgtt gaaacacgtt 10740
cttcttgtcg atgacgacgt cgctatgcgg catcttatta ttgaatacct tacgatccac 10800
gccttcaaag tgaccgcggt agccgacagc acccagttca caagagtact ctcttccgcg 10860
acggtcgatg tcgtggttgt tgatctagat ttaggtcgtg aagatgggct cgagatcgtt 10920
cgtaatctgg cggcaaagtc tgatattcca atcataatta tcagtggcga ccgccttgag 10980
gagacggata aagttgttgc actcgagcta ggagcaagtg attttatcgc taagccgttc 11040
agtatcagag agtttctagc acgcattcgg gttgccttgc gcgtgcgccc caacgttgtc 11100
cgctccaaag accgacggtc tttttgtttt actgactgga cacttaatct caggcaacgt 11160
cgcttgatgt ccgaagctgg cggtgaggtg aaacttacgg caggtgagtt caatcttctc 11220
ctcgcgtttt tagagaaacc ccgcgacgtt ctatcgcgcg agcaacttct cattgccagt 11280
cgagtacgcg acgaggaggt ttatgacagg agtatagatg ttctcatttt gaggctgcgc 11340
cgcaaacttg aggcagatcc gtcaagccct caactgataa aaacagcaag aggtgccggt 11400
tatttctttg acgcggacgt gcaggtttcg cacgggggga cgatggcagc ctgagccaat 11460
tcccagatcc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg 11520
gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc 11580
aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc 11640
gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg 11700
gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc 11760
gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg 11820
tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg 11880
ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga 11940
accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg 12000
acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa 12060
cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg 12120
gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga 12180
gcgaaaccgg gcggccggag tacatcgaga tcgagctggc tgattggatg taccgcgaga 12240
tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc 12300
ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca 12360
gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct 12420
gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg 12480
aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag 12540
catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa 12600
aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca 12660
ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca 12720
tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac 12780
ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg 12840
aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc 12900
gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac 12960
cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgctgaggt ctgcctcgtg 13020
aagaaggtgt tgctgactca taccaggcct gaatcgcccc atcatccagc cagaaagtga 13080
gggagccacg gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt 13140
gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag 13200
caaaagttcg atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa tgctctgcca 13260
gtgttacaac caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg 13320
caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga 13380
aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat 13440
tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc 13500
aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gctctgcatt 13560
aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 13620
cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 13680
aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 13740
aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 13800
tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 13860
caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 13920
cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 13980
ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 14040
gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 14100
agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 14160
gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 14220
acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 14280
gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 14340
gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 14400
cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 14460
caaaaaggat cttcacctag atccttttga tccggaatta 14500
<210> 108
<211> 14510
<212> DNA
<213> 人工序列
<220>
<223> 23713载体
<400> 108
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctagtata cgcacacaca tgttgagata ttagaggaaa ctgaagaatt 1260
acgccacact aggtacgccg ctcgtcctcc cccccccccc ctctctacct tctctagatc 1320
ggcgttccgg tccatagtta gggcccggta gttctacttc tgttcatgtt tgtgttagat 1380
ccgtgtttgt gttagatccg tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag 1440
acacgttctg attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag 1500
ccgttccgca gacgggatcg atttcatgat tttttttgtt tcgttgcata gggtttggtt 1560
tgcccttttc ctttatttca atatatgccg tgcacttgtt tgtcgggtca tcttttcatg 1620
cttttttttg tcttggttgt gatgatgtgg tctggttggg cggtcgttct agatcggagt 1680
agaattctgt ttcaaactac ctggtggatt tattaatttt ggatctgtat gtgtgtgcca 1740
tacatattca tagttacgaa ttgaagatga tggatggaaa tatcgatcta ggataggtat 1800
acatgttgat gcgggtttta ctgatgcata tacagagatg ctttttgttc gcttggttgt 1860
gatgatgtgg tgtggttggg cggtcgttca ttcgttctag atcggagtag aatactgttt 1920
caaactacct ggtgtattta ttaattttgg aactgtatgt gtgtgtcata catcttcata 1980
gttacgagtt taagatggat ggaaatatcg atctaggata ggtatacatg ttgatgtggg 2040
ttttactgat gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga 2100
gtacctatct attataataa acaagtatgt tttataatta ttttgatctt gatatacttg 2160
gatgatggca tatccagcag ctatatgtgg atttttttag ccctgccttc atacgctatt 2220
tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc 2280
aggatcctct aagtagcagc catgaacaag aacaacacca agctgagcac ccgcgccctg 2340
ccgagcttca tcgactactt caacggcatc tacggcttcg ccaccggcat caaggacatc 2400
atgaacatga tcttcaagac cgacaccggc ggcgacctga ccctggacga gatcctgaag 2460
aaccagcagc tgctgaacga catcagcggc aagctggacg gcgtgaacgg cagcctgaac 2520
gacctgatcg cccagggcaa cctgaacacc gagctgagca aggagatcct taagatcgcc 2580
aacgagcaga accaggtgct gaacgacgtg aacaacaagc tggacgccat caacaccatg 2640
ctgcgcgtgt acctgccgaa gatcaccagc atgctgagcg acgtgattaa gcagaactac 2700
gccctgagcc tgcagatcga gtacctgagc aagcagctgc aggagatcag cgacaagctg 2760
gacatcatca acgtgaacgt cctgatcaac agcaccctga ccgagatcac cccggcctac 2820
cagcgcatca agtacgtgaa cgagaagttc gaagagctga ccttcgccac cgagaccagc 2880
agcaaggtga agaaggacgg cagcccggcc gacatcctgg acgagctgac cgagctgacc 2940
gagctggcga agagcgtgac caagaacgac gtggacggct tcgagttcta cctgaacacc 3000
ttccacgacg tgatggtggg caacaacctg ttcggccgca gcgccctgaa gaccgccagc 3060
gagctgatca ccaaggagaa cgtgaagacc agcggcagcg aggtgggcaa cgtgtacaac 3120
ttcctgatcg tgctgaccgc cctgcaggcc caggccttcc tgaccctgac cacctgtcgc 3180
aagctgctgg gcctggccga catcgactac accagcatca tgaacgagca cttgaacaag 3240
gagaaggagg agttccgcgt gaacatcctg ccgaccctga gcaacacctt cagcaacccg 3300
aactacgcca aggtgaaggg cagcgacgag gacgccaaga tgatcgtgga ggctaagccg 3360
ggccacgcgt tgatcggctt cgagatcagc aacgacagca tcaccgtgct gaaggtgtac 3420
gaggccaagc tgaagcagaa ctaccaggtg gacaaggaca gcttgagcga ggtgatctac 3480
ggcgacatgg acaagctgct gtgtccggac cagagcgagc aaatctacta caccaacaac 3540
atcgtgttcc cgaacgagta cgtgatcacc aagatcgact tcaccaagaa gatgaagacc 3600
ctgcgctacg aggtgaccgc caacttctac gacagcagca ccggcgagat cgacctgaac 3660
aagaagaagg tggagagcag cgaggccgag taccgcaccc tgagcgcgaa cgacgacggc 3720
gtctacatgc cactgggcgt gatcagcgag accttcctga ccccgatcaa cggctttggc 3780
ctgcaggccg acgagaacag ccgcctgatc accctgacct gtaagagcta cctgcgcgag 3840
ctgctgctag ccaccgacct gagcaacaag gagaccaagc tgatcgtgcc accgagcggc 3900
ttcatcagca acatcgtgga gaacggcagc atcgaggagg acaacctgga gccgtggaag 3960
gccaacaaca agaacgccta cgtcgaccac accggcggcg tgaacggcac caaggccctg 4020
tacgtgcaca aggacggcgg catcagccag ttcatcggcg acaagctgaa gccgaagacc 4080
gagtacgtga tccagtacac cgtgaagggc aagccatcga ttcacctgaa ggacgagaac 4140
accggctaca tccactacga ggacaccaac aacaacctgg aggactacca gaccatcaac 4200
aagcgcttca ccaccggcac cgacctgaag ggcgtgtacc tgatcctgaa gagccagaac 4260
ggcgacgagg cctggggcga caacttcatc atcctggaga tcagcccgag cgagaagctg 4320
ctgagcccgg agctgatcaa caccaacaac tggaccagca ccggcagcac caacatcagc 4380
ggcaacaccc tgaccctgta ccagggcggc cgcggcatcc tgaagcagaa cctgcagctg 4440
gacagcttca gcacctaccg cgtgtacttc agcgtgagcg gcgacgccaa cgtgcgcatc 4500
cgcaactccc gcgaggtgct gttcgagaag aggtacatga gcggcgccaa ggacgtgagc 4560
gagatgttca ccaccaagtt cgagaaggac aacttctaca tcgagctgag ccagggcaac 4620
aacctgtacg gcggcccgat cgtgcacttc tacgacgtga gcatcaagta ggagctctag 4680
atcttatctg cacaaagtgg agtagtcagt catcgatcag gaaccagaca ccagactttt 4740
attcatacag tgaagtgaag tgaagtgcag tgcagtgagt tgctggtttt tgtacaactt 4800
agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa 4860
aatccaggct acacggaccg cgatcgctta attaagcttg catgcctgca gtgcagcgtg 4920
acccggtcgt gcccctctct agagataatg agcattgcat gtctaagtta taaaaaatta 4980
ccacatattt tttttgtcac acttgtttga agtgcagttt atctatcttt atacatatat 5040
ttaaacttta ctctacgaat aatataatct atagtactac aataatatca gtgttttaga 5100
gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt ttgacaacag 5160
gactctacag ttttatcttt ttagtgtgca tgtgttctcc tttttttttg caaatagctt 5220
cacctatata atacttcatc cattttatta gtacatccat ttagggttta gggttaatgg 5280
tttttataga ctaatttttt tagtacatct attttattct attttagcct ctaaattaag 5340
aaaactaaaa ctctatttta gtttttttat ttaataattt agatataaaa tagaataaaa 5400
taaagtgact aaaaattaaa caaataccct ttaagaaatt aaaaaaacta aggaaacatt 5460
tttcttgttt cgagtagata atgccagcct gttaaacgcc gccgacgagt ctaacggaca 5520
ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca cggcatctct 5580
gtcgctgcct ctggacccct ctcgagagtt ccgctccacc gttggacttg ctccgctgtc 5640
ggcatccaga aattgcgtgg cggagcggca gacgtgagcc ggcacggcag gcggcctcct 5700
cctcctctca cggcaccggc agctacgggg gattcctttc ccaccgctcc ttcgctttcc 5760
cttcctcgcc cgccgtaata aatagacacc ccctccacac cctctttccc caacctcgtg 5820
ttgttcggag cgcacacaca cacaaccaga tctcccccaa atccacccgt cggcacctcc 5880
gcttcaaggt acgccgctcg tcctcccccc ccccccctct ctaccttctc tagatcggcg 5940
ttccggtcca tagttagggc ccggtagttc tacttctgtt catgtttgtg ttagatccgt 6000
gtttgtgtta gatccgtgct gttagcgttc gtacacggat gcgacctgta cgtcagacac 6060
gttctgattg ctaacttgcc agtgtttctc tttggggaat cctgggatgg ctctagccgt 6120
tccgcagacg ggatcgattt catgattttt tttgtttcgt tgcatagggt ttggtttgcc 6180
cttttccttt atttcaatat atgccgtgca cttgtttgtc gggtcatctt ttcatgcttt 6240
tttttgtctt ggttgtgatg atgtggtctg gttgggcggt cgttctagat cggagtagaa 6300
ttctgtttca aactacctgg tggatttatt aattttggat ctgtatgtgt gtgccataca 6360
tattcatagt tacgaattga agatgatgga tggaaatatc gatctaggat aggtatacat 6420
gttgatgcgg gttttactga tgcatataca gagatgcttt ttgttcgctt ggttgtgatg 6480
atgtggtgtg gttgggcggt cgttcattcg ttctagatcg gagtagaata ctgtttcaaa 6540
ctacctggtg tatttattaa ttttggaact gtatgtgtgt gtcatacatc ttcatagtta 6600
cgagtttaag atggatggaa atatcgatct aggataggta tacatgttga tgtgggtttt 6660
actgatgcat atacatgatg gcatatgcag catctattca tatgctctaa ccttgagtac 6720
ctatctatta taataaacaa gtatgtttta taattatttt gatcttgata tacttggatg 6780
atggcatatg cagcagctat atgtggattt ttttagccct gccttcatac gctatttatt 6840
tgcttggtac tgtttctttt gtcgatgctc accctgttgt ttggtgttac ttctgcaggg 6900
atctccgatc atgcaaaaac tcattaactc agtgcaaaac tatgcctggg gcagcaaaac 6960
ggcgttgact gaactttatg gtatggaaaa tccgtccagc cagccgatgg ccgagctgtg 7020
gatgggcgca catccgaaaa gcagttcacg agtgcagaat gccgccggag atatcgtttc 7080
actgcgtgat gtgattgaga gtgataaatc gactctgctc ggagaggccg ttgccaaacg 7140
ctttggcgaa ctgcctttcc tgttcaaagt attatgcgca gcacagccac tctccattca 7200
ggttcatcca aacaaacaca attctgaaat cggttttgcc aaagaaaatg ccgcaggtat 7260
cccgatggat gccgccgagc gtaactataa agatcctaac cacaagccgg agctggtttt 7320
tgcgctgacg cctttccttg cgatgaacgc gtttcgtgaa ttttccgaga ttgtctccct 7380
actccagccg gtcgcaggtg cacatccggc gattgctcac tttttacaac agcctgatgc 7440
cgaacgttta agcgaactgt tcgccagcct gttgaatatg cagggtgaag aaaaatcccg 7500
cgcgctggcg attttaaaat cggccctcga tagccagcag ggtgaaccgt ggcaaacgat 7560
tcgtttaatt tctgaatttt acccggaaga cagcggtctg ttctccccgc tattgctgaa 7620
tgtggtgaaa ttgaaccctg gcgaagcgat gttcctgttc gctgaaacac cgcacgctta 7680
cctgcaaggc gtggcgctgg aagtgatggc aaactccgat aacgtgctgc gtgcgggtct 7740
gacgcctaaa tacattgata ttccggaact ggttgccaat gtgaaattcg aagccaaacc 7800
ggctaaccag ttgttgaccc agccggtgaa acaaggtgca gaactggact tcccgattcc 7860
agtggatgat tttgccttct cgctgcatga ccttagtgat aaagaaacca ccattagcca 7920
gcagagtgcc gccattttgt tctgcgtcga aggcgatgca acgttgtgga aaggttctca 7980
gcagttacag cttaaaccgg gtgaatcagc gtttattgcc gccaacgaat caccggtgac 8040
tgtcaaaggc cacggccgtt tagcgcgtgt ttacaacaag ctgtaagagc ttactgaaaa 8100
aattaacatc tcttgctaag ctgggtcatg ggtcgtttaa gctgccgatg tgcctgcgtc 8160
gtctggtgcc ctctctccat atggaggttg tcaaagtatc tgctgttcgt gtcatgagtc 8220
gtgtcagtgt tggtttaata atggaccggt tgtgttgtgt gtgcgtacta cccagaacta 8280
tgacaaatca tgaataagtt tgatgtttga aattaaagcc tgtgctcatt atgttctgtc 8340
tttcagttgt ctcctaatat ttgcctccag gtactggcta tctaccgttt cttacttagg 8400
aggtgtttga atgcactaaa actaatagtt agtggctaaa attagttaaa acatccaaac 8460
accatagcta atagttgaac tattagctat ttttggaaaa ttagttaata gtgaggtagt 8520
tatttgttag ctagctaatt caactaacaa tttttagcca actaacaatt agtttcagtg 8580
cattcaaaca cccccttaat gttaacgtgg ttctatctac cgtctcctaa tatatggttg 8640
attgttcggt ttgttgctat gctattgggt tctgattgct gctagttctt gctgaatcca 8700
gaagttctcg tagtatagct cagattcata ttatttattt gagtgataag tgatccaggt 8760
tattactatg ttagctaggt tttttttaca aggataaatt atctgtgatc ataattctta 8820
tgaaagcttt atgtttcctg gaggcagtgg catgcaatgc atgacagcaa cttgatcaca 8880
ccagctgagg tagatacggt aacaaggttc ttaaatctgt tcaccaaatc attggagaac 8940
acacatacac attcttgcca gtcttggtta gagaaatttc atgacaaaat gccaaagctg 9000
tcttgactct tcacttttgg ccatgagtcg tgacttagtt tggtttaatg gaccggttct 9060
cctagcttgt tctactcaaa actgttgttg atgcgaataa gttgtgatgg ttgatctctg 9120
gattttgttt tgctctcaat agtggacgag attagatagc ctgcaggccc gggggcgcgc 9180
cctaattagc taacggccag gatcgccgcg tgagccttta gcaactagct agattaatta 9240
acgcaatctg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc 9300
accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg 9360
cagcccatca gaattaattc tcatgtttga cagcttatca tcgactgcac ggtgcaccaa 9420
tgcttctggc gtcaggcagc catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac 9480
tgcataattc gtgtcgctca aggcgcactc ccgttctgga taatgttttt tgcgccgaca 9540
tcataacggt tctggcaaat attctgaaat gagctgttga caattaatca tccggctcgt 9600
ataatgtgtg gaattgtgag cggataacaa tttcacacag gaaacagacc atgagggaag 9660
cgttgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc 9720
tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc 9780
cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc 9840
gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc 9900
gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta 9960
agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc 10020
cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg 10080
ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg 10140
aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc 10200
gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc 10260
cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca 10320
tacttgaagc taggcaggct tatcttggac aagaagatcg cttggcctcg cgcgcagatc 10380
agttggaaga atttgttcac tacgtgaaag gcgagatcac caaagtagtc ggcaaataaa 10440
gctctagtgg atctccgtac ccagggatct ggctcgcggc ggacgcacga cgccggggcg 10500
agaccatagg cgatctccta aatcaatagt agctgtaacc tcgaagcgtt tcacttgtaa 10560
caacgattga gaatttttgt cataaaattg aaatacttgg ttcgcatttt tgtcatccgc 10620
ggtcagccgc aattctgacg aactgcccat ttagctggag atgattgtac atccttcacg 10680
tgaaaatttc tcaagcgctg tgaacaaggg ttcagatttt agattgaaag gtgagccgtt 10740
gaaacacgtt cttcttgtcg atgacgacgt cgctatgcgg catcttatta ttgaatacct 10800
tacgatccac gccttcaaag tgaccgcggt agccgacagc acccagttca caagagtact 10860
ctcttccgcg acggtcgatg tcgtggttgt tgatctagat ttaggtcgtg aagatgggct 10920
cgagatcgtt cgtaatctgg cggcaaagtc tgatattcca atcataatta tcagtggcga 10980
ccgccttgag gagacggata aagttgttgc actcgagcta ggagcaagtg attttatcgc 11040
taagccgttc agtatcagag agtttctagc acgcattcgg gttgccttgc gcgtgcgccc 11100
caacgttgtc cgctccaaag accgacggtc tttttgtttt actgactgga cacttaatct 11160
caggcaacgt cgcttgatgt ccgaagctgg cggtgaggtg aaacttacgg caggtgagtt 11220
caatcttctc ctcgcgtttt tagagaaacc ccgcgacgtt ctatcgcgcg agcaacttct 11280
cattgccagt cgagtacgcg acgaggaggt ttatgacagg agtatagatg ttctcatttt 11340
gaggctgcgc cgcaaacttg aggcagatcc gtcaagccct caactgataa aaacagcaag 11400
aggtgccggt tatttctttg acgcggacgt gcaggtttcg cacgggggga cgatggcagc 11460
ctgagccaat tcccagatcc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg 11520
gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc 11580
gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct 11640
gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag 11700
ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc 11760
cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga 11820
gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg 11880
gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc 11940
gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca 12000
cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac 12060
ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag 12120
gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag 12180
atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctggc tgattggatg 12240
taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt 12300
ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag 12360
gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc 12420
aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat 12480
ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc 12540
gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta 12600
gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca 12660
aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac 12720
cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac 12780
tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag 12840
cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc 12900
gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca 12960
ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgctgaggt 13020
ctgcctcgtg aagaaggtgt tgctgactca taccaggcct gaatcgcccc atcatccagc 13080
cagaaagtga gggagccacg gttgatgaga gctttgttgt aggtggacca gttggtgatt 13140
ttgaactttt gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc 13200
ttcaactcag caaaagttcg atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa 13260
tgctctgcca gtgttacaac caattaacca attctgatta gaaaaactca tcgagcatca 13320
aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt 13380
tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc 13440
ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa 13500
taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa 13560
gctctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 13620
tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 13680
gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 13740
atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 13800
ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 13860
cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 13920
tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 13980
gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 14040
aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 14100
tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 14160
aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 14220
aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 14280
ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 14340
ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 14400
atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 14460
atgagattat caaaaaggat cttcacctag atccttttga tccggaatta 14510
<210> 109
<211> 16488
<212> DNA
<213> 人工序列
<220>
<223> 23714载体
<400> 109
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta 1260
gatcggcgtt ccggtccata gttagggccc ggtagttcta cttctgttca tgtttgtgtt 1320
agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg 1380
tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct 1440
ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt 1500
ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt 1560
catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg 1620
gagtagaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt 1680
gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag 1740
gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg 1800
ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact 1860
gtttcaaact acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt 1920
catagttacg agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg 1980
tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc 2040
ttgagtacct atctattata ataaacaagt atgttttata attattttga tcttgatata 2100
cttggatgat ggcatatcca gcagctatat gtggattttt ttagccctgc cttcatacgc 2160
tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt 2220
ctgcaggatc ctctaagtag cagccatgaa caagaacaac accaagctga gcacccgcgc 2280
cctgccgagc ttcatcgact acttcaacgg catctacggc ttcgccaccg gcatcaagga 2340
catcatgaac atgatcttca agaccgacac cggcggcgac ctgaccctgg acgagatcct 2400
gaagaaccag cagctgctga acgacatcag cggcaagctg gacggcgtga acggcagcct 2460
gaacgacctg atcgcccagg gcaacctgaa caccgagctg agcaaggaga tccttaagat 2520
cgccaacgag cagaaccagg tgctgaacga cgtgaacaac aagctggacg ccatcaacac 2580
catgctgcgc gtgtacctgc cgaagatcac cagcatgctg agcgacgtga ttaagcagaa 2640
ctacgccctg agcctgcaga tcgagtacct gagcaagcag ctgcaggaga tcagcgacaa 2700
gctggacatc atcaacgtga acgtcctgat caacagcacc ctgaccgaga tcaccccggc 2760
ctaccagcgc atcaagtacg tgaacgagaa gttcgaagag ctgaccttcg ccaccgagac 2820
cagcagcaag gtgaagaagg acggcagccc ggccgacatc ctggacgagc tgaccgagct 2880
gaccgagctg gcgaagagcg tgaccaagaa cgacgtggac ggcttcgagt tctacctgaa 2940
caccttccac gacgtgatgg tgggcaacaa cctgttcggc cgcagcgccc tgaagaccgc 3000
cagcgagctg atcaccaagg agaacgtgaa gaccagcggc agcgaggtgg gcaacgtgta 3060
caacttcctg atcgtgctga ccgccctgca ggcccaggcc ttcctgaccc tgaccacctg 3120
tcgcaagctg ctgggcctgg ccgacatcga ctacaccagc atcatgaacg agcacttgaa 3180
caaggagaag gaggagttcc gcgtgaacat cctgccgacc ctgagcaaca ccttcagcaa 3240
cccgaactac gccaaggtga agggcagcga cgaggacgcc aagatgatcg tggaggctaa 3300
gccgggccac gcgttgatcg gcttcgagat cagcaacgac agcatcaccg tgctgaaggt 3360
gtacgaggcc aagctgaagc agaactacca ggtggacaag gacagcttga gcgaggtgat 3420
ctacggcgac atggacaagc tgctgtgtcc ggaccagagc gagcaaatct actacaccaa 3480
caacatcgtg ttcccgaacg agtacgtgat caccaagatc gacttcacca agaagatgaa 3540
gaccctgcgc tacgaggtga ccgccaactt ctacgacagc agcaccggcg agatcgacct 3600
gaacaagaag aaggtggaga gcagcgaggc cgagtaccgc accctgagcg cgaacgacga 3660
cggcgtctac atgccactgg gcgtgatcag cgagaccttc ctgaccccga tcaacggctt 3720
tggcctgcag gccgacgaga acagccgcct gatcaccctg acctgtaaga gctacctgcg 3780
cgagctgctg ctagccaccg acctgagcaa caaggagacc aagctgatcg tgccaccgag 3840
cggcttcatc agcaacatcg tggagaacgg cagcatcgag gaggacaacc tggagccgtg 3900
gaaggccaac aacaagaacg cctacgtcga ccacaccggc ggcgtgaacg gcaccaaggc 3960
cctgtacgtg cacaaggacg gcggcatcag ccagttcatc ggcgacaagc tgaagccgaa 4020
gaccgagtac gtgatccagt acaccgtgaa gggcaagcca tcgattcacc tgaaggacga 4080
gaacaccggc tacatccact acgaggacac caacaacaac ctggaggact accagaccat 4140
caacaagcgc ttcaccaccg gcaccgacct gaagggcgtg tacctgatcc tgaagagcca 4200
gaacggcgac gaggcctggg gcgacaactt catcatcctg gagatcagcc cgagcgagaa 4260
gctgctgagc ccggagctga tcaacaccaa caactggacc agcaccggca gcaccaacat 4320
cagcggcaac accctgaccc tgtaccaggg cggccgcggc atcctgaagc agaacctgca 4380
gctggacagc ttcagcacct accgcgtgta cttcagcgtg agcggcgacg ccaacgtgcg 4440
catccgcaac tcccgcgagg tgctgttcga gaagaggtac atgagcggcg ccaaggacgt 4500
gagcgagatg ttcaccacca agttcgagaa ggacaacttc tacatcgagc tgagccaggg 4560
caacaacctg tacggcggcc cgatcgtgca cttctacgac gtgagcatca agtaggagct 4620
ctatacgcac acacatgttg agatattaga ggaaactgaa gaattacgcc acattatctg 4680
cacaaagtgg agtagtcagt catcgatcag gaaccagaca ccagactttt attcatacag 4740
tgaagtgaag tgaagtgcag tgcagtgagt tgctggtttt tgtacaactt agtatgtatt 4800
tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccaggct 4860
acacggaccg cgatcgctga cgaggctgat ataatttagg gctggttcgg ttaggggtgg 4920
attgagaagg attgaagggg attaaatatc ctcctattga aatttgaata ggtccaatcc 4980
acatcaatct atctcaaact gaataaggct ttagggctga tttgttgatc aaggatcacg 5040
agagattgaa atcccctcca acaccctgtg atcctcttgt caccaaatca atctagggag 5100
tgtttggctc ccaccaccac cttctagact aaaattttag tcttttagtt tgtaaactac 5160
taaacagtat gactaagatt ctgtttggag gcaattaaat ttttaagaaa ctgatttttt 5220
ttatctttta gttaggaggg gctagcttct tggtttttaa gaaactatga atcaaatttt 5280
tataaactga ggcataaact gatatgtttg aaacaacctc aatttccata aacaattttt 5340
aaaaaaattg ggcaaatagg ctctaaatgt agtctcacaa atataaattt tagggactaa 5400
agtgtagttc ttaaagttta gaaggatgac taaagaaaca agaattcccc atgctcgatt 5460
tttttttaca gagaaaaaac atttttttaa gtttaaccaa gtatatagaa aatgttttat 5520
atctaaataa gttcattctg aaagtatact ccactatcat tctaacgata attactacgc 5580
atcgttattt cctaatataa attagagtgc agtgactttg taacaccgtg gagtgatgaa 5640
ctatttctca aagtttggtc atggtgaact gaactaaaaa gccagagcag ctagctccac 5700
gcgagtgcca gaacaaggcg ccgtgctttt gcagcttttc aacaagggtc aagggggcag 5760
caaggcatac tctgtgtgat ttgtgaaggg cagcagcccg ggtaggtgta actgaaaaac 5820
agatgctgct gctgctctcc tgagtcctga actcccaacg gtgcctgcct tctctaggag 5880
gcctataaat ttgggtcgag cagcagagca acgccttcac agccaatgca gggtagcttc 5940
tcacctccaa acagctagtc tctgttctta cccattccac cctgttacga aaagctagaa 6000
aagtgtcagg cactgaaagt gagagttgga ggaaagcaaa ccggctgggt atcggattca 6060
gcaacggagg gtgctgccgg tcggtttgtt ttcctccaat atctcacctt cagtcgatag 6120
tctgatgaag atgtacacta ccaacatcca ttcatttgtg ccatctgaag tgaggattag 6180
aggcaactga accatcatct attgcaaagg ttcagtttcc tctaatatct catctcgaat 6240
gggcccatcg ccggagatgt ttgtttgcat atagctgaaa atgcagtcac ccaaagtgag 6300
tgctgaaagg aatttctaac acctaggttg aattgagtga tactgaagtg tttggccatc 6360
tgctagtact aaccactagt caaacagatg gcaatccacg ttgtcatcga gttggctgaa 6420
cagatttgct cggaaaggta tacattctcc atttgggaca tgtgtgggag ccaggggatg 6480
gagcagattg gaggggattt tagcctctcg aatcccctcc atttcgctgg ctcctaaaca 6540
agccgccctt gaagtgctga tttgaggtaa gtaaaccgac cggagaagca gggacaaaga 6600
agtatcgttt catcagatgt gatgcaccaa cttctaccgt tattttgaca aagcactgca 6660
tgtaaatctg cagtggttta tccaatagca aacttgttct ctactttgtc actgtactaa 6720
ctgaaactga cgaacatccc cacttgtatt acaattttat ctcgatctgc agggtaagac 6780
agctttcgcg tgtatacaca caagtcgaga aaacccctca ttcgtatgca ttaacttgag 6840
agctagaaat ctagaatgcg atcgcttaat taagcttgca tgcctgcagt gcagcgtgac 6900
ccggtcgtgc ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc 6960
acatattttt tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt 7020
aaactttact ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga 7080
atcatataaa tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga 7140
ctctacagtt ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca 7200
cctatataat acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt 7260
tttatagact aattttttta gtacatctat tttattctat tttagcctct aaattaagaa 7320
aactaaaact ctattttagt ttttttattt aataatttag atataaaata gaataaaata 7380
aagtgactaa aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt 7440
tcttgtttcg agtagataat gccagcctgt taaacgccgc cgacgagtct aacggacacc 7500
aaccagcgaa ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt 7560
cgctgcctct ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg 7620
catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc 7680
tcctctcacg gcaccggcag ctacggggga ttcctttccc accgctcctt cgctttccct 7740
tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt 7800
gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc 7860
ttcaaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt 7920
ccggtccata gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt 7980
ttgtgttaga tccgtgctgt tagcgttcgt acacggatgc gacctgtacg tcagacacgt 8040
tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc 8100
cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt ggtttgccct 8160
tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt catgcttttt 8220
tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg gagtagaatt 8280
ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata 8340
ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt 8400
tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat 8460
gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact gtttcaaact 8520
acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg 8580
agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac 8640
tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct 8700
atctattata ataaacaagt atgttttata attattttga tcttgatata cttggatgat 8760
ggcatatgca gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg 8820
cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt ctgcagggat 8880
ctccgatcat gcaaaaactc attaactcag tgcaaaacta tgcctggggc agcaaaacgg 8940
cgttgactga actttatggt atggaaaatc cgtccagcca gccgatggcc gagctgtgga 9000
tgggcgcaca tccgaaaagc agttcacgag tgcagaatgc cgccggagat atcgtttcac 9060
tgcgtgatgt gattgagagt gataaatcga ctctgctcgg agaggccgtt gccaaacgct 9120
ttggcgaact gcctttcctg ttcaaagtat tatgcgcagc acagccactc tccattcagg 9180
ttcatccaaa caaacacaat tctgaaatcg gttttgccaa agaaaatgcc gcaggtatcc 9240
cgatggatgc cgccgagcgt aactataaag atcctaacca caagccggag ctggtttttg 9300
cgctgacgcc tttccttgcg atgaacgcgt ttcgtgaatt ttccgagatt gtctccctac 9360
tccagccggt cgcaggtgca catccggcga ttgctcactt tttacaacag cctgatgccg 9420
aacgtttaag cgaactgttc gccagcctgt tgaatatgca gggtgaagaa aaatcccgcg 9480
cgctggcgat tttaaaatcg gccctcgata gccagcaggg tgaaccgtgg caaacgattc 9540
gtttaatttc tgaattttac ccggaagaca gcggtctgtt ctccccgcta ttgctgaatg 9600
tggtgaaatt gaaccctggc gaagcgatgt tcctgttcgc tgaaacaccg cacgcttacc 9660
tgcaaggcgt ggcgctggaa gtgatggcaa actccgataa cgtgctgcgt gcgggtctga 9720
cgcctaaata cattgatatt ccggaactgg ttgccaatgt gaaattcgaa gccaaaccgg 9780
ctaaccagtt gttgacccag ccggtgaaac aaggtgcaga actggacttc ccgattccag 9840
tggatgattt tgccttctcg ctgcatgacc ttagtgataa agaaaccacc attagccagc 9900
agagtgccgc cattttgttc tgcgtcgaag gcgatgcaac gttgtggaaa ggttctcagc 9960
agttacagct taaaccgggt gaatcagcgt ttattgccgc caacgaatca ccggtgactg 10020
tcaaaggcca cggccgttta gcgcgtgttt acaacaagct gtaagagctt actgaaaaaa 10080
ttaacatctc ttgctaagct gggtcatggg tcgtttaagc tgccgatgtg cctgcgtcgt 10140
ctggtgccct ctctccatat ggaggttgtc aaagtatctg ctgttcgtgt catgagtcgt 10200
gtcagtgttg gtttaataat ggaccggttg tgttgtgtgt gcgtactacc cagaactatg 10260
acaaatcatg aataagtttg atgtttgaaa ttaaagcctg tgctcattat gttctgtctt 10320
tcagttgtct cctaatattt gcctccaggt actggctatc taccgtttct tacttaggag 10380
gtgtttgaat gcactaaaac taatagttag tggctaaaat tagttaaaac atccaaacac 10440
catagctaat agttgaacta ttagctattt ttggaaaatt agttaatagt gaggtagtta 10500
tttgttagct agctaattca actaacaatt tttagccaac taacaattag tttcagtgca 10560
ttcaaacacc cccttaatgt taacgtggtt ctatctaccg tctcctaata tatggttgat 10620
tgttcggttt gttgctatgc tattgggttc tgattgctgc tagttcttgc tgaatccaga 10680
agttctcgta gtatagctca gattcatatt atttatttga gtgataagtg atccaggtta 10740
ttactatgtt agctaggttt tttttacaag gataaattat ctgtgatcat aattcttatg 10800
aaagctttat gtttcctgga ggcagtggca tgcaatgcat gacagcaact tgatcacacc 10860
agctgaggta gatacggtaa caaggttctt aaatctgttc accaaatcat tggagaacac 10920
acatacacat tcttgccagt cttggttaga gaaatttcat gacaaaatgc caaagctgtc 10980
ttgactcttc acttttggcc atgagtcgtg acttagtttg gtttaatgga ccggttctcc 11040
tagcttgttc tactcaaaac tgttgttgat gcgaataagt tgtgatggtt gatctctgga 11100
ttttgttttg ctctcaatag tggacgagat tagatagcct gcaggcccgg gggcgcgccc 11160
taattagcta acggccagga tcgccgcgtg agcctttagc aactagctag attaattaac 11220
gcaatctgtt attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac 11280
cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca 11340
gcccatcaga attaattctc atgtttgaca gcttatcatc gactgcacgg tgcaccaatg 11400
cttctggcgt caggcagcca tcggaagctg tggtatggct gtgcaggtcg taaatcactg 11460
cataattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc 11520
ataacggttc tggcaaatat tctgaaatga gctgttgaca attaatcatc cggctcgtat 11580
aatgtgtgga attgtgagcg gataacaatt tcacacagga aacagaccat gagggaagcg 11640
ttgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc 11700
gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca 11760
cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga 11820
gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc 11880
gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag 11940
cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca 12000
gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc 12060
ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag 12120
gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga 12180
aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg 12240
aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata 12300
cttgaagcta ggcaggctta tcttggacaa gaagatcgct tggcctcgcg cgcagatcag 12360
ttggaagaat ttgttcacta cgtgaaaggc gagatcacca aagtagtcgg caaataaagc 12420
tctagtggat ctccgtaccc agggatctgg ctcgcggcgg acgcacgacg ccggggcgag 12480
accataggcg atctcctaaa tcaatagtag ctgtaacctc gaagcgtttc acttgtaaca 12540
acgattgaga atttttgtca taaaattgaa atacttggtt cgcatttttg tcatccgcgg 12600
tcagccgcaa ttctgacgaa ctgcccattt agctggagat gattgtacat ccttcacgtg 12660
aaaatttctc aagcgctgtg aacaagggtt cagattttag attgaaaggt gagccgttga 12720
aacacgttct tcttgtcgat gacgacgtcg ctatgcggca tcttattatt gaatacctta 12780
cgatccacgc cttcaaagtg accgcggtag ccgacagcac ccagttcaca agagtactct 12840
cttccgcgac ggtcgatgtc gtggttgttg atctagattt aggtcgtgaa gatgggctcg 12900
agatcgttcg taatctggcg gcaaagtctg atattccaat cataattatc agtggcgacc 12960
gccttgagga gacggataaa gttgttgcac tcgagctagg agcaagtgat tttatcgcta 13020
agccgttcag tatcagagag tttctagcac gcattcgggt tgccttgcgc gtgcgcccca 13080
acgttgtccg ctccaaagac cgacggtctt tttgttttac tgactggaca cttaatctca 13140
ggcaacgtcg cttgatgtcc gaagctggcg gtgaggtgaa acttacggca ggtgagttca 13200
atcttctcct cgcgttttta gagaaacccc gcgacgttct atcgcgcgag caacttctca 13260
ttgccagtcg agtacgcgac gaggaggttt atgacaggag tatagatgtt ctcattttga 13320
ggctgcgccg caaacttgag gcagatccgt caagccctca actgataaaa acagcaagag 13380
gtgccggtta tttctttgac gcggacgtgc aggtttcgca cggggggacg atggcagcct 13440
gagccaattc ccagatcccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt 13500
acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc 13560
ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga 13620
tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc 13680
gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg 13740
cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc 13800
tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc 13860
cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga 13920
atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca 13980
cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct 14040
ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc 14100
caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat 14160
cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctggctg attggatgta 14220
ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt 14280
gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc 14340
agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa 14400
gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt 14460
gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga 14520
gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc 14580
aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa 14640
gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg 14700
gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc 14760
tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg 14820
cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc 14880
cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg 14940
caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gctgaggtct 15000
gcctcgtgaa gaaggtgttg ctgactcata ccaggcctga atcgccccat catccagcca 15060
gaaagtgagg gagccacggt tgatgagagc tttgttgtag gtggaccagt tggtgatttt 15120
gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt 15180
caactcagca aaagttcgat ttattcaaca aagccgccgt cccgtcaagt cagcgtaatg 15240
ctctgccagt gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa 15300
tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc 15360
tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg 15420
tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata 15480
aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc 15540
tctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 15600
cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 15660
tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 15720
gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 15780
ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 15840
aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 15900
tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 15960
ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 16020
gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 16080
tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 16140
caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 16200
ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 16260
cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 16320
ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 16380
cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 16440
gagattatca aaaaggatct tcacctagat ccttttgatc cggaatta 16488
<210> 110
<211> 14500
<212> DNA
<213> 人工序列
<220>
<223> 23715载体
<400> 110
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta 1260
gatcggcgtt ccggtccata gttagggccc ggtagttcta cttctgttca tgtttgtgtt 1320
agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg 1380
tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct 1440
ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt 1500
ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt 1560
catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg 1620
gagtagaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt 1680
gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag 1740
gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg 1800
ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact 1860
gtttcaaact acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt 1920
catagttacg agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg 1980
tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc 2040
ttgagtacct atctattata ataaacaagt atgttttata attattttga tcttgatata 2100
cttggatgat ggcatatcca gcagctatat gtggattttt ttagccctgc cttcatacgc 2160
tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt 2220
ctgcaggatc ctctaagtag cagccatgaa caagaacaac accaagctga gcacccgcgc 2280
cctgccgagc ttcatcgact acttcaacgg catctacggc ttcgccaccg gcatcaagga 2340
catcatgaac atgatcttca agaccgacac cggcggcgac ctgaccctgg acgagatcct 2400
gaagaaccag cagctgctga acgacatcag cggcaagctg gacggcgtga acggcagcct 2460
gaacgacctg atcgcccagg gcaacctgaa caccgagctg agcaaggaga tccttaagat 2520
cgccaacgag cagaaccagg tgctgaacga cgtgaacaac aagctggacg ccatcaacac 2580
catgctgcgc gtgtacctgc cgaagatcac cagcatgctg agcgacgtga ttaagcagaa 2640
ctacgccctg agcctgcaga tcgagtacct gagcaagcag ctgcaggaga tcagcgacaa 2700
gctggacatc atcaacgtga acgtcctgat caacagcacc ctgaccgaga tcaccccggc 2760
ctaccagcgc atcaagtacg tgaacgagaa gttcgaagag ctgaccttcg ccaccgagac 2820
cagcagcaag gtgaagaagg acggcagccc ggccgacatc ctggacgagc tgaccgagct 2880
gaccgagctg gcgaagagcg tgaccaagaa cgacgtggac ggcttcgagt tctacctgaa 2940
caccttccac gacgtgatgg tgggcaacaa cctgttcggc cgcagcgccc tgaagaccgc 3000
cagcgagctg atcaccaagg agaacgtgaa gaccagcggc agcgaggtgg gcaacgtgta 3060
caacttcctg atcgtgctga ccgccctgca ggcccaggcc ttcctgaccc tgaccacctg 3120
tcgcaagctg ctgggcctgg ccgacatcga ctacaccagc atcatgaacg agcacttgaa 3180
caaggagaag gaggagttcc gcgtgaacat cctgccgacc ctgagcaaca ccttcagcaa 3240
cccgaactac gccaaggtga agggcagcga cgaggacgcc aagatgatcg tggaggctaa 3300
gccgggccac gcgttgatcg gcttcgagat cagcaacgac agcatcaccg tgctgaaggt 3360
gtacgaggcc aagctgaagc agaactacca ggtggacaag gacagcttga gcgaggtgat 3420
ctacggcgac atggacaagc tgctgtgtcc ggaccagagc gagcaaatct actacaccaa 3480
caacatcgtg ttcccgaacg agtacgtgat caccaagatc gacttcacca agaagatgaa 3540
gaccctgcgc tacgaggtga ccgccaactt ctacgacagc agcaccggcg agatcgacct 3600
gaacaagaag aaggtggaga gcagcgaggc cgagtaccgc accctgagcg cgaacgacga 3660
cggcgtctac atgccactgg gcgtgatcag cgagaccttc ctgaccccga tcaacggctt 3720
tggcctgcag gccgacgaga acagccgcct gatcaccctg acctgtaaga gctacctgcg 3780
cgagctgctg ctagccaccg acctgagcaa caaggagacc aagctgatcg tgccaccgag 3840
cggcttcatc agcaacatcg tggagaacgg cagcatcgag gaggacaacc tggagccgtg 3900
gaaggccaac aacaagaacg cctacgtcga ccacaccggc ggcgtgaacg gcaccaaggc 3960
cctgtacgtg cacaaggacg gcggcatcag ccagttcatc ggcgacaagc tgaagccgaa 4020
gaccgagtac gtgatccagt acaccgtgaa gggcaagcca tcgattcacc tgaaggacga 4080
gaacaccggc tacatccact acgaggacac caacaacaac ctggaggact accagaccat 4140
caacaagcgc ttcaccaccg gcaccgacct gaagggcgtg tacctgatcc tgaagagcca 4200
gaacggcgac gaggcctggg gcgacaactt catcatcctg gagatcagcc cgagcgagaa 4260
gctgctgagc ccggagctga tcaacaccaa caactggacc agcaccggca gcaccaacat 4320
cagcggcaac accctgaccc tgtaccaggg cggccgcggc atcctgaagc agaacctgca 4380
gctggacagc ttcagcacct accgcgtgta cttcagcgtg agcggcgacg ccaacgtgcg 4440
catccgcaac tcccgcgagg tgctgttcga gaagaggtac atgagcggcg ccaaggacgt 4500
gagcgagatg ttcaccacca agttcgagaa ggacaacttc tacatcgagc tgagccaggg 4560
caacaacctg tacggcggcc cgatcgtgca cttctacgac gtgagcatca agtaggagct 4620
ccgccaaatg aatcttcttg agattttaga gtaaactgaa actgactgtt gcattatctg 4680
cacaaagtgg agtagtcagt catcgatcag gaaccagaca ccagactttt attcatacag 4740
tgaagtgaag tgaagtgcag tgcagtgagt tgctggtttt tgtacaactt agtatgtatt 4800
tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccaggct 4860
acacggaccg cgatcgctta attaagcttg catgcctgca gtgcagcgtg acccggtcgt 4920
gcccctctct agagataatg agcattgcat gtctaagtta taaaaaatta ccacatattt 4980
tttttgtcac acttgtttga agtgcagttt atctatcttt atacatatat ttaaacttta 5040
ctctacgaat aatataatct atagtactac aataatatca gtgttttaga gaatcatata 5100
aatgaacagt tagacatggt ctaaaggaca attgagtatt ttgacaacag gactctacag 5160
ttttatcttt ttagtgtgca tgtgttctcc tttttttttg caaatagctt cacctatata 5220
atacttcatc cattttatta gtacatccat ttagggttta gggttaatgg tttttataga 5280
ctaatttttt tagtacatct attttattct attttagcct ctaaattaag aaaactaaaa 5340
ctctatttta gtttttttat ttaataattt agatataaaa tagaataaaa taaagtgact 5400
aaaaattaaa caaataccct ttaagaaatt aaaaaaacta aggaaacatt tttcttgttt 5460
cgagtagata atgccagcct gttaaacgcc gccgacgagt ctaacggaca ccaaccagcg 5520
aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca cggcatctct gtcgctgcct 5580
ctggacccct ctcgagagtt ccgctccacc gttggacttg ctccgctgtc ggcatccaga 5640
aattgcgtgg cggagcggca gacgtgagcc ggcacggcag gcggcctcct cctcctctca 5700
cggcaccggc agctacgggg gattcctttc ccaccgctcc ttcgctttcc cttcctcgcc 5760
cgccgtaata aatagacacc ccctccacac cctctttccc caacctcgtg ttgttcggag 5820
cgcacacaca cacaaccaga tctcccccaa atccacccgt cggcacctcc gcttcaaggt 5880
acgccgctcg tcctcccccc ccccccctct ctaccttctc tagatcggcg ttccggtcca 5940
tagttagggc ccggtagttc tacttctgtt catgtttgtg ttagatccgt gtttgtgtta 6000
gatccgtgct gttagcgttc gtacacggat gcgacctgta cgtcagacac gttctgattg 6060
ctaacttgcc agtgtttctc tttggggaat cctgggatgg ctctagccgt tccgcagacg 6120
ggatcgattt catgattttt tttgtttcgt tgcatagggt ttggtttgcc cttttccttt 6180
atttcaatat atgccgtgca cttgtttgtc gggtcatctt ttcatgcttt tttttgtctt 6240
ggttgtgatg atgtggtctg gttgggcggt cgttctagat cggagtagaa ttctgtttca 6300
aactacctgg tggatttatt aattttggat ctgtatgtgt gtgccataca tattcatagt 6360
tacgaattga agatgatgga tggaaatatc gatctaggat aggtatacat gttgatgcgg 6420
gttttactga tgcatataca gagatgcttt ttgttcgctt ggttgtgatg atgtggtgtg 6480
gttgggcggt cgttcattcg ttctagatcg gagtagaata ctgtttcaaa ctacctggtg 6540
tatttattaa ttttggaact gtatgtgtgt gtcatacatc ttcatagtta cgagtttaag 6600
atggatggaa atatcgatct aggataggta tacatgttga tgtgggtttt actgatgcat 6660
atacatgatg gcatatgcag catctattca tatgctctaa ccttgagtac ctatctatta 6720
taataaacaa gtatgtttta taattatttt gatcttgata tacttggatg atggcatatg 6780
cagcagctat atgtggattt ttttagccct gccttcatac gctatttatt tgcttggtac 6840
tgtttctttt gtcgatgctc accctgttgt ttggtgttac ttctgcaggg atctccgatc 6900
atgcaaaaac tcattaactc agtgcaaaac tatgcctggg gcagcaaaac ggcgttgact 6960
gaactttatg gtatggaaaa tccgtccagc cagccgatgg ccgagctgtg gatgggcgca 7020
catccgaaaa gcagttcacg agtgcagaat gccgccggag atatcgtttc actgcgtgat 7080
gtgattgaga gtgataaatc gactctgctc ggagaggccg ttgccaaacg ctttggcgaa 7140
ctgcctttcc tgttcaaagt attatgcgca gcacagccac tctccattca ggttcatcca 7200
aacaaacaca attctgaaat cggttttgcc aaagaaaatg ccgcaggtat cccgatggat 7260
gccgccgagc gtaactataa agatcctaac cacaagccgg agctggtttt tgcgctgacg 7320
cctttccttg cgatgaacgc gtttcgtgaa ttttccgaga ttgtctccct actccagccg 7380
gtcgcaggtg cacatccggc gattgctcac tttttacaac agcctgatgc cgaacgttta 7440
agcgaactgt tcgccagcct gttgaatatg cagggtgaag aaaaatcccg cgcgctggcg 7500
attttaaaat cggccctcga tagccagcag ggtgaaccgt ggcaaacgat tcgtttaatt 7560
tctgaatttt acccggaaga cagcggtctg ttctccccgc tattgctgaa tgtggtgaaa 7620
ttgaaccctg gcgaagcgat gttcctgttc gctgaaacac cgcacgctta cctgcaaggc 7680
gtggcgctgg aagtgatggc aaactccgat aacgtgctgc gtgcgggtct gacgcctaaa 7740
tacattgata ttccggaact ggttgccaat gtgaaattcg aagccaaacc ggctaaccag 7800
ttgttgaccc agccggtgaa acaaggtgca gaactggact tcccgattcc agtggatgat 7860
tttgccttct cgctgcatga ccttagtgat aaagaaacca ccattagcca gcagagtgcc 7920
gccattttgt tctgcgtcga aggcgatgca acgttgtgga aaggttctca gcagttacag 7980
cttaaaccgg gtgaatcagc gtttattgcc gccaacgaat caccggtgac tgtcaaaggc 8040
cacggccgtt tagcgcgtgt ttacaacaag ctgtaagagc ttactgaaaa aattaacatc 8100
tcttgctaag ctgggtcatg ggtcgtttaa gctgccgatg tgcctgcgtc gtctggtgcc 8160
ctctctccat atggaggttg tcaaagtatc tgctgttcgt gtcatgagtc gtgtcagtgt 8220
tggtttaata atggaccggt tgtgttgtgt gtgcgtacta cccagaacta tgacaaatca 8280
tgaataagtt tgatgtttga aattaaagcc tgtgctcatt atgttctgtc tttcagttgt 8340
ctcctaatat ttgcctccag gtactggcta tctaccgttt cttacttagg aggtgtttga 8400
atgcactaaa actaatagtt agtggctaaa attagttaaa acatccaaac accatagcta 8460
atagttgaac tattagctat ttttggaaaa ttagttaata gtgaggtagt tatttgttag 8520
ctagctaatt caactaacaa tttttagcca actaacaatt agtttcagtg cattcaaaca 8580
cccccttaat gttaacgtgg ttctatctac cgtctcctaa tatatggttg attgttcggt 8640
ttgttgctat gctattgggt tctgattgct gctagttctt gctgaatcca gaagttctcg 8700
tagtatagct cagattcata ttatttattt gagtgataag tgatccaggt tattactatg 8760
ttagctaggt tttttttaca aggataaatt atctgtgatc ataattctta tgaaagcttt 8820
atgtttcctg gaggcagtgg catgcaatgc atgacagcaa cttgatcaca ccagctgagg 8880
tagatacggt aacaaggttc ttaaatctgt tcaccaaatc attggagaac acacatacac 8940
attcttgcca gtcttggtta gagaaatttc atgacaaaat gccaaagctg tcttgactct 9000
tcacttttgg ccatgagtcg tgacttagtt tggtttaatg gaccggttct cctagcttgt 9060
tctactcaaa actgttgttg atgcgaataa gttgtgatgg ttgatctctg gattttgttt 9120
tgctctcaat agtggacgag attagatagc ctgcaggccc gggggcgcgc cctaattagc 9180
taacggccag gatcgccgcg tgagccttta gcaactagct agattaatta acgcaatctg 9240
ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc 9300
caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca 9360
gaattaattc tcatgtttga cagcttatca tcgactgcac ggtgcaccaa tgcttctggc 9420
gtcaggcagc catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac tgcataattc 9480
gtgtcgctca aggcgcactc ccgttctgga taatgttttt tgcgccgaca tcataacggt 9540
tctggcaaat attctgaaat gagctgttga caattaatca tccggctcgt ataatgtgtg 9600
gaattgtgag cggataacaa tttcacacag gaaacagacc atgagggaag cgttgatcgc 9660
cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac 9720
gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga 9780
tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat 9840
caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga 9900
agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact 9960
gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat 10020
cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg 10080
tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa 10140
tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt 10200
gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt 10260
cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc 10320
taggcaggct tatcttggac aagaagatcg cttggcctcg cgcgcagatc agttggaaga 10380
atttgttcac tacgtgaaag gcgagatcac caaagtagtc ggcaaataaa gctctagtgg 10440
atctccgtac ccagggatct ggctcgcggc ggacgcacga cgccggggcg agaccatagg 10500
cgatctccta aatcaatagt agctgtaacc tcgaagcgtt tcacttgtaa caacgattga 10560
gaatttttgt cataaaattg aaatacttgg ttcgcatttt tgtcatccgc ggtcagccgc 10620
aattctgacg aactgcccat ttagctggag atgattgtac atccttcacg tgaaaatttc 10680
tcaagcgctg tgaacaaggg ttcagatttt agattgaaag gtgagccgtt gaaacacgtt 10740
cttcttgtcg atgacgacgt cgctatgcgg catcttatta ttgaatacct tacgatccac 10800
gccttcaaag tgaccgcggt agccgacagc acccagttca caagagtact ctcttccgcg 10860
acggtcgatg tcgtggttgt tgatctagat ttaggtcgtg aagatgggct cgagatcgtt 10920
cgtaatctgg cggcaaagtc tgatattcca atcataatta tcagtggcga ccgccttgag 10980
gagacggata aagttgttgc actcgagcta ggagcaagtg attttatcgc taagccgttc 11040
agtatcagag agtttctagc acgcattcgg gttgccttgc gcgtgcgccc caacgttgtc 11100
cgctccaaag accgacggtc tttttgtttt actgactgga cacttaatct caggcaacgt 11160
cgcttgatgt ccgaagctgg cggtgaggtg aaacttacgg caggtgagtt caatcttctc 11220
ctcgcgtttt tagagaaacc ccgcgacgtt ctatcgcgcg agcaacttct cattgccagt 11280
cgagtacgcg acgaggaggt ttatgacagg agtatagatg ttctcatttt gaggctgcgc 11340
cgcaaacttg aggcagatcc gtcaagccct caactgataa aaacagcaag aggtgccggt 11400
tatttctttg acgcggacgt gcaggtttcg cacgggggga cgatggcagc ctgagccaat 11460
tcccagatcc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg 11520
gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc 11580
aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc 11640
gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg 11700
gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc 11760
gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg 11820
tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg 11880
ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga 11940
accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg 12000
acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa 12060
cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg 12120
gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga 12180
gcgaaaccgg gcggccggag tacatcgaga tcgagctggc tgattggatg taccgcgaga 12240
tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc 12300
ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca 12360
gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct 12420
gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg 12480
aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag 12540
catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa 12600
aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca 12660
ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca 12720
tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac 12780
ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg 12840
aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc 12900
gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac 12960
cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgctgaggt ctgcctcgtg 13020
aagaaggtgt tgctgactca taccaggcct gaatcgcccc atcatccagc cagaaagtga 13080
gggagccacg gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt 13140
gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag 13200
caaaagttcg atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa tgctctgcca 13260
gtgttacaac caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg 13320
caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga 13380
aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat 13440
tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc 13500
aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gctctgcatt 13560
aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 13620
cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 13680
aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 13740
aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 13800
tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 13860
caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 13920
cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 13980
ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 14040
gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 14100
agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 14160
gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 14220
acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 14280
gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 14340
gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 14400
cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 14460
caaaaaggat cttcacctag atccttttga tccggaatta 14500
<210> 111
<211> 14508
<212> DNA
<213> 人工序列
<220>
<223> 23716载体
<400> 111
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta 1260
gatcggcgtt ccggtccata gttagggccc ggtagttcta cttctgttca tgtttgtgtt 1320
agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg 1380
tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct 1440
ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt 1500
ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt 1560
catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg 1620
gagtagaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt 1680
gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag 1740
gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg 1800
ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact 1860
gtttcaaact acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt 1920
catagttacg agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg 1980
tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc 2040
ttgagtacct atctattata ataaacaagt atgttttata attattttga tcttgatata 2100
cttggatgat ggcatatcca gcagctatat gtggattttt ttagccctgc cttcatacgc 2160
tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt 2220
ctgcaggatc ctctaagtag cagccatgta tacgcacaca catgttgaga tattagagga 2280
aactgaagaa ttacgccaca ttaacaagaa caacaccaag ctgagcaccc gcgccctgcc 2340
gagcttcatc gactacttca acggcatcta cggcttcgcc accggcatca aggacatcat 2400
gaacatgatc ttcaagaccg acaccggcgg cgacctgacc ctggacgaga tcctgaagaa 2460
ccagcagctg ctgaacgaca tcagcggcaa gctggacggc gtgaacggca gcctgaacga 2520
cctgatcgcc cagggcaacc tgaacaccga gctgagcaag gagatcctta agatcgccaa 2580
cgagcagaac caggtgctga acgacgtgaa caacaagctg gacgccatca acaccatgct 2640
gcgcgtgtac ctgccgaaga tcaccagcat gctgagcgac gtgattaagc agaactacgc 2700
cctgagcctg cagatcgagt acctgagcaa gcagctgcag gagatcagcg acaagctgga 2760
catcatcaac gtgaacgtcc tgatcaacag caccctgacc gagatcaccc cggcctacca 2820
gcgcatcaag tacgtgaacg agaagttcga agagctgacc ttcgccaccg agaccagcag 2880
caaggtgaag aaggacggca gcccggccga catcctggac gagctgaccg agctgaccga 2940
gctggcgaag agcgtgacca agaacgacgt ggacggcttc gagttctacc tgaacacctt 3000
ccacgacgtg atggtgggca acaacctgtt cggccgcagc gccctgaaga ccgccagcga 3060
gctgatcacc aaggagaacg tgaagaccag cggcagcgag gtgggcaacg tgtacaactt 3120
cctgatcgtg ctgaccgccc tgcaggccca ggccttcctg accctgacca cctgtcgcaa 3180
gctgctgggc ctggccgaca tcgactacac cagcatcatg aacgagcact tgaacaagga 3240
gaaggaggag ttccgcgtga acatcctgcc gaccctgagc aacaccttca gcaacccgaa 3300
ctacgccaag gtgaagggca gcgacgagga cgccaagatg atcgtggagg ctaagccggg 3360
ccacgcgttg atcggcttcg agatcagcaa cgacagcatc accgtgctga aggtgtacga 3420
ggccaagctg aagcagaact accaggtgga caaggacagc ttgagcgagg tgatctacgg 3480
cgacatggac aagctgctgt gtccggacca gagcgagcaa atctactaca ccaacaacat 3540
cgtgttcccg aacgagtacg tgatcaccaa gatcgacttc accaagaaga tgaagaccct 3600
gcgctacgag gtgaccgcca acttctacga cagcagcacc ggcgagatcg acctgaacaa 3660
gaagaaggtg gagagcagcg aggccgagta ccgcaccctg agcgcgaacg acgacggcgt 3720
ctacatgcca ctgggcgtga tcagcgagac cttcctgacc ccgatcaacg gctttggcct 3780
gcaggccgac gagaacagcc gcctgatcac cctgacctgt aagagctacc tgcgcgagct 3840
gctgctagcc accgacctga gcaacaagga gaccaagctg atcgtgccac cgagcggctt 3900
catcagcaac atcgtggaga acggcagcat cgaggaggac aacctggagc cgtggaaggc 3960
caacaacaag aacgcctacg tcgaccacac cggcggcgtg aacggcacca aggccctgta 4020
cgtgcacaag gacggcggca tcagccagtt catcggcgac aagctgaagc cgaagaccga 4080
gtacgtgatc cagtacaccg tgaagggcaa gccatcgatt cacctgaagg acgagaacac 4140
cggctacatc cactacgagg acaccaacaa caacctggag gactaccaga ccatcaacaa 4200
gcgcttcacc accggcaccg acctgaaggg cgtgtacctg atcctgaaga gccagaacgg 4260
cgacgaggcc tggggcgaca acttcatcat cctggagatc agcccgagcg agaagctgct 4320
gagcccggag ctgatcaaca ccaacaactg gaccagcacc ggcagcacca acatcagcgg 4380
caacaccctg accctgtacc agggcggccg cggcatcctg aagcagaacc tgcagctgga 4440
cagcttcagc acctaccgcg tgtacttcag cgtgagcggc gacgccaacg tgcgcatccg 4500
caactcccgc gaggtgctgt tcgagaagag gtacatgagc ggcgccaagg acgtgagcga 4560
gatgttcacc accaagttcg agaaggacaa cttctacatc gagctgagcc agggcaacaa 4620
cctgtacggc ggcccgatcg tgcacttcta cgacgtgagc atcaagtagg agctctagat 4680
cttatctgca caaagtggag tagtcagtca tcgatcagga accagacacc agacttttat 4740
tcatacagtg aagtgaagtg aagtgcagtg cagtgagttg ctggtttttg tacaacttag 4800
tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa 4860
tccaggctac acggaccgcg atcgcttaat taagcttgca tgcctgcagt gcagcgtgac 4920
ccggtcgtgc ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc 4980
acatattttt tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt 5040
aaactttact ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga 5100
atcatataaa tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga 5160
ctctacagtt ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca 5220
cctatataat acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt 5280
tttatagact aattttttta gtacatctat tttattctat tttagcctct aaattaagaa 5340
aactaaaact ctattttagt ttttttattt aataatttag atataaaata gaataaaata 5400
aagtgactaa aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt 5460
tcttgtttcg agtagataat gccagcctgt taaacgccgc cgacgagtct aacggacacc 5520
aaccagcgaa ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt 5580
cgctgcctct ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg 5640
catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc 5700
tcctctcacg gcaccggcag ctacggggga ttcctttccc accgctcctt cgctttccct 5760
tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt 5820
gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc 5880
ttcaaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt 5940
ccggtccata gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt 6000
ttgtgttaga tccgtgctgt tagcgttcgt acacggatgc gacctgtacg tcagacacgt 6060
tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc 6120
cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt ggtttgccct 6180
tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt catgcttttt 6240
tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg gagtagaatt 6300
ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata 6360
ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt 6420
tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat 6480
gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact gtttcaaact 6540
acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg 6600
agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac 6660
tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct 6720
atctattata ataaacaagt atgttttata attattttga tcttgatata cttggatgat 6780
ggcatatgca gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg 6840
cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt ctgcagggat 6900
ctccgatcat gcaaaaactc attaactcag tgcaaaacta tgcctggggc agcaaaacgg 6960
cgttgactga actttatggt atggaaaatc cgtccagcca gccgatggcc gagctgtgga 7020
tgggcgcaca tccgaaaagc agttcacgag tgcagaatgc cgccggagat atcgtttcac 7080
tgcgtgatgt gattgagagt gataaatcga ctctgctcgg agaggccgtt gccaaacgct 7140
ttggcgaact gcctttcctg ttcaaagtat tatgcgcagc acagccactc tccattcagg 7200
ttcatccaaa caaacacaat tctgaaatcg gttttgccaa agaaaatgcc gcaggtatcc 7260
cgatggatgc cgccgagcgt aactataaag atcctaacca caagccggag ctggtttttg 7320
cgctgacgcc tttccttgcg atgaacgcgt ttcgtgaatt ttccgagatt gtctccctac 7380
tccagccggt cgcaggtgca catccggcga ttgctcactt tttacaacag cctgatgccg 7440
aacgtttaag cgaactgttc gccagcctgt tgaatatgca gggtgaagaa aaatcccgcg 7500
cgctggcgat tttaaaatcg gccctcgata gccagcaggg tgaaccgtgg caaacgattc 7560
gtttaatttc tgaattttac ccggaagaca gcggtctgtt ctccccgcta ttgctgaatg 7620
tggtgaaatt gaaccctggc gaagcgatgt tcctgttcgc tgaaacaccg cacgcttacc 7680
tgcaaggcgt ggcgctggaa gtgatggcaa actccgataa cgtgctgcgt gcgggtctga 7740
cgcctaaata cattgatatt ccggaactgg ttgccaatgt gaaattcgaa gccaaaccgg 7800
ctaaccagtt gttgacccag ccggtgaaac aaggtgcaga actggacttc ccgattccag 7860
tggatgattt tgccttctcg ctgcatgacc ttagtgataa agaaaccacc attagccagc 7920
agagtgccgc cattttgttc tgcgtcgaag gcgatgcaac gttgtggaaa ggttctcagc 7980
agttacagct taaaccgggt gaatcagcgt ttattgccgc caacgaatca ccggtgactg 8040
tcaaaggcca cggccgttta gcgcgtgttt acaacaagct gtaagagctt actgaaaaaa 8100
ttaacatctc ttgctaagct gggtcatggg tcgtttaagc tgccgatgtg cctgcgtcgt 8160
ctggtgccct ctctccatat ggaggttgtc aaagtatctg ctgttcgtgt catgagtcgt 8220
gtcagtgttg gtttaataat ggaccggttg tgttgtgtgt gcgtactacc cagaactatg 8280
acaaatcatg aataagtttg atgtttgaaa ttaaagcctg tgctcattat gttctgtctt 8340
tcagttgtct cctaatattt gcctccaggt actggctatc taccgtttct tacttaggag 8400
gtgtttgaat gcactaaaac taatagttag tggctaaaat tagttaaaac atccaaacac 8460
catagctaat agttgaacta ttagctattt ttggaaaatt agttaatagt gaggtagtta 8520
tttgttagct agctaattca actaacaatt tttagccaac taacaattag tttcagtgca 8580
ttcaaacacc cccttaatgt taacgtggtt ctatctaccg tctcctaata tatggttgat 8640
tgttcggttt gttgctatgc tattgggttc tgattgctgc tagttcttgc tgaatccaga 8700
agttctcgta gtatagctca gattcatatt atttatttga gtgataagtg atccaggtta 8760
ttactatgtt agctaggttt tttttacaag gataaattat ctgtgatcat aattcttatg 8820
aaagctttat gtttcctgga ggcagtggca tgcaatgcat gacagcaact tgatcacacc 8880
agctgaggta gatacggtaa caaggttctt aaatctgttc accaaatcat tggagaacac 8940
acatacacat tcttgccagt cttggttaga gaaatttcat gacaaaatgc caaagctgtc 9000
ttgactcttc acttttggcc atgagtcgtg acttagtttg gtttaatgga ccggttctcc 9060
tagcttgttc tactcaaaac tgttgttgat gcgaataagt tgtgatggtt gatctctgga 9120
ttttgttttg ctctcaatag tggacgagat tagatagcct gcaggcccgg gggcgcgccc 9180
taattagcta acggccagga tcgccgcgtg agcctttagc aactagctag attaattaac 9240
gcaatctgtt attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac 9300
cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca 9360
gcccatcaga attaattctc atgtttgaca gcttatcatc gactgcacgg tgcaccaatg 9420
cttctggcgt caggcagcca tcggaagctg tggtatggct gtgcaggtcg taaatcactg 9480
cataattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc 9540
ataacggttc tggcaaatat tctgaaatga gctgttgaca attaatcatc cggctcgtat 9600
aatgtgtgga attgtgagcg gataacaatt tcacacagga aacagaccat gagggaagcg 9660
ttgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc 9720
gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca 9780
cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga 9840
gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc 9900
gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag 9960
cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca 10020
gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc 10080
ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag 10140
gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga 10200
aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg 10260
aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata 10320
cttgaagcta ggcaggctta tcttggacaa gaagatcgct tggcctcgcg cgcagatcag 10380
ttggaagaat ttgttcacta cgtgaaaggc gagatcacca aagtagtcgg caaataaagc 10440
tctagtggat ctccgtaccc agggatctgg ctcgcggcgg acgcacgacg ccggggcgag 10500
accataggcg atctcctaaa tcaatagtag ctgtaacctc gaagcgtttc acttgtaaca 10560
acgattgaga atttttgtca taaaattgaa atacttggtt cgcatttttg tcatccgcgg 10620
tcagccgcaa ttctgacgaa ctgcccattt agctggagat gattgtacat ccttcacgtg 10680
aaaatttctc aagcgctgtg aacaagggtt cagattttag attgaaaggt gagccgttga 10740
aacacgttct tcttgtcgat gacgacgtcg ctatgcggca tcttattatt gaatacctta 10800
cgatccacgc cttcaaagtg accgcggtag ccgacagcac ccagttcaca agagtactct 10860
cttccgcgac ggtcgatgtc gtggttgttg atctagattt aggtcgtgaa gatgggctcg 10920
agatcgttcg taatctggcg gcaaagtctg atattccaat cataattatc agtggcgacc 10980
gccttgagga gacggataaa gttgttgcac tcgagctagg agcaagtgat tttatcgcta 11040
agccgttcag tatcagagag tttctagcac gcattcgggt tgccttgcgc gtgcgcccca 11100
acgttgtccg ctccaaagac cgacggtctt tttgttttac tgactggaca cttaatctca 11160
ggcaacgtcg cttgatgtcc gaagctggcg gtgaggtgaa acttacggca ggtgagttca 11220
atcttctcct cgcgttttta gagaaacccc gcgacgttct atcgcgcgag caacttctca 11280
ttgccagtcg agtacgcgac gaggaggttt atgacaggag tatagatgtt ctcattttga 11340
ggctgcgccg caaacttgag gcagatccgt caagccctca actgataaaa acagcaagag 11400
gtgccggtta tttctttgac gcggacgtgc aggtttcgca cggggggacg atggcagcct 11460
gagccaattc ccagatcccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt 11520
acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc 11580
ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga 11640
tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc 11700
gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg 11760
cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc 11820
tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc 11880
cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga 11940
atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca 12000
cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct 12060
ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc 12120
caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat 12180
cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctggctg attggatgta 12240
ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt 12300
gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc 12360
agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa 12420
gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt 12480
gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga 12540
gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc 12600
aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa 12660
gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg 12720
gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc 12780
tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg 12840
cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc 12900
cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg 12960
caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gctgaggtct 13020
gcctcgtgaa gaaggtgttg ctgactcata ccaggcctga atcgccccat catccagcca 13080
gaaagtgagg gagccacggt tgatgagagc tttgttgtag gtggaccagt tggtgatttt 13140
gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt 13200
caactcagca aaagttcgat ttattcaaca aagccgccgt cccgtcaagt cagcgtaatg 13260
ctctgccagt gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa 13320
tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc 13380
tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg 13440
tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata 13500
aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc 13560
tctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 13620
cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 13680
tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 13740
gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 13800
ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 13860
aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 13920
tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 13980
ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 14040
gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 14100
tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 14160
caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 14220
ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 14280
cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 14340
ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 14400
cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 14460
gagattatca aaaaggatct tcacctagat ccttttgatc cggaatta 14508
<210> 112
<211> 14478
<212> DNA
<213> 人工序列
<220>
<223> 23717载体
<400> 112
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta 1260
gatcggcgtt ccggtccata gttagggccc ggtagttcta cttctgttca tgtttgtgtt 1320
agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg 1380
tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct 1440
ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt 1500
ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt 1560
catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg 1620
gagtagaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt 1680
gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag 1740
gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg 1800
ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact 1860
gtttcaaact acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt 1920
catagttacg agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg 1980
tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc 2040
ttgagtacct atctattata ataaacaagt atgttttata attattttga tcttgatata 2100
cttggatgat ggcatatcca gcagctatat gtggattttt ttagccctgc cttcatacgc 2160
tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt 2220
ctgcaggatc ctctaagtag cagccatggc tgagatatta gaggaaactg aaaacaagaa 2280
caacaccaag ctgagcaccc gcgccctgcc gagcttcatc gactacttca acggcatcta 2340
cggcttcgcc accggcatca aggacatcat gaacatgatc ttcaagaccg acaccggcgg 2400
cgacctgacc ctggacgaga tcctgaagaa ccagcagctg ctgaacgaca tcagcggcaa 2460
gctggacggc gtgaacggca gcctgaacga cctgatcgcc cagggcaacc tgaacaccga 2520
gctgagcaag gagatcctta agatcgccaa cgagcagaac caggtgctga acgacgtgaa 2580
caacaagctg gacgccatca acaccatgct gcgcgtgtac ctgccgaaga tcaccagcat 2640
gctgagcgac gtgattaagc agaactacgc cctgagcctg cagatcgagt acctgagcaa 2700
gcagctgcag gagatcagcg acaagctgga catcatcaac gtgaacgtcc tgatcaacag 2760
caccctgacc gagatcaccc cggcctacca gcgcatcaag tacgtgaacg agaagttcga 2820
agagctgacc ttcgccaccg agaccagcag caaggtgaag aaggacggca gcccggccga 2880
catcctggac gagctgaccg agctgaccga gctggcgaag agcgtgacca agaacgacgt 2940
ggacggcttc gagttctacc tgaacacctt ccacgacgtg atggtgggca acaacctgtt 3000
cggccgcagc gccctgaaga ccgccagcga gctgatcacc aaggagaacg tgaagaccag 3060
cggcagcgag gtgggcaacg tgtacaactt cctgatcgtg ctgaccgccc tgcaggccca 3120
ggccttcctg accctgacca cctgtcgcaa gctgctgggc ctggccgaca tcgactacac 3180
cagcatcatg aacgagcact tgaacaagga gaaggaggag ttccgcgtga acatcctgcc 3240
gaccctgagc aacaccttca gcaacccgaa ctacgccaag gtgaagggca gcgacgagga 3300
cgccaagatg atcgtggagg ctaagccggg ccacgcgttg atcggcttcg agatcagcaa 3360
cgacagcatc accgtgctga aggtgtacga ggccaagctg aagcagaact accaggtgga 3420
caaggacagc ttgagcgagg tgatctacgg cgacatggac aagctgctgt gtccggacca 3480
gagcgagcaa atctactaca ccaacaacat cgtgttcccg aacgagtacg tgatcaccaa 3540
gatcgacttc accaagaaga tgaagaccct gcgctacgag gtgaccgcca acttctacga 3600
cagcagcacc ggcgagatcg acctgaacaa gaagaaggtg gagagcagcg aggccgagta 3660
ccgcaccctg agcgcgaacg acgacggcgt ctacatgcca ctgggcgtga tcagcgagac 3720
cttcctgacc ccgatcaacg gctttggcct gcaggccgac gagaacagcc gcctgatcac 3780
cctgacctgt aagagctacc tgcgcgagct gctgctagcc accgacctga gcaacaagga 3840
gaccaagctg atcgtgccac cgagcggctt catcagcaac atcgtggaga acggcagcat 3900
cgaggaggac aacctggagc cgtggaaggc caacaacaag aacgcctacg tcgaccacac 3960
cggcggcgtg aacggcacca aggccctgta cgtgcacaag gacggcggca tcagccagtt 4020
catcggcgac aagctgaagc cgaagaccga gtacgtgatc cagtacaccg tgaagggcaa 4080
gccatcgatt cacctgaagg acgagaacac cggctacatc cactacgagg acaccaacaa 4140
caacctggag gactaccaga ccatcaacaa gcgcttcacc accggcaccg acctgaaggg 4200
cgtgtacctg atcctgaaga gccagaacgg cgacgaggcc tggggcgaca acttcatcat 4260
cctggagatc agcccgagcg agaagctgct gagcccggag ctgatcaaca ccaacaactg 4320
gaccagcacc ggcagcacca acatcagcgg caacaccctg accctgtacc agggcggccg 4380
cggcatcctg aagcagaacc tgcagctgga cagcttcagc acctaccgcg tgtacttcag 4440
cgtgagcggc gacgccaacg tgcgcatccg caactcccgc gaggtgctgt tcgagaagag 4500
gtacatgagc ggcgccaagg acgtgagcga gatgttcacc accaagttcg agaaggacaa 4560
cttctacatc gagctgagcc agggcaacaa cctgtacggc ggcccgatcg tgcacttcta 4620
cgacgtgagc atcaagtagg agctctagat cttatctgca caaagtggag tagtcagtca 4680
tcgatcagga accagacacc agacttttat tcatacagtg aagtgaagtg aagtgcagtg 4740
cagtgagttg ctggtttttg tacaacttag tatgtatttg tatttgtaaa atacttctat 4800
caataaaatt tctaattcct aaaaccaaaa tccaggctac acggaccgcg atcgcttaat 4860
taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc ccctctctag agataatgag 4920
cattgcatgt ctaagttata aaaaattacc acatattttt tttgtcacac ttgtttgaag 4980
tgcagtttat ctatctttat acatatattt aaactttact ctacgaataa tataatctat 5040
agtactacaa taatatcagt gttttagaga atcatataaa tgaacagtta gacatggtct 5100
aaaggacaat tgagtatttt gacaacagga ctctacagtt ttatcttttt agtgtgcatg 5160
tgttctcctt tttttttgca aatagcttca cctatataat acttcatcca ttttattagt 5220
acatccattt agggtttagg gttaatggtt tttatagact aattttttta gtacatctat 5280
tttattctat tttagcctct aaattaagaa aactaaaact ctattttagt ttttttattt 5340
aataatttag atataaaata gaataaaata aagtgactaa aaattaaaca aatacccttt 5400
aagaaattaa aaaaactaag gaaacatttt tcttgtttcg agtagataat gccagcctgt 5460
taaacgccgc cgacgagtct aacggacacc aaccagcgaa ccagcagcgt cgcgtcgggc 5520
caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct cgagagttcc 5580
gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga 5640
cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga 5700
ttcctttccc accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc 5760
ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc 5820
tcccccaaat ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc 5880
ccccctctct accttctcta gatcggcgtt ccggtccata gttagggccc ggtagttcta 5940
cttctgttca tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgt tagcgttcgt 6000
acacggatgc gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt 6060
tggggaatcc tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt 6120
tgtttcgttg catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact 6180
tgtttgtcgg gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt 6240
tgggcggtcg ttctagatcg gagtagaatt ctgtttcaaa ctacctggtg gatttattaa 6300
ttttggatct gtatgtgtgt gccatacata ttcatagtta cgaattgaag atgatggatg 6360
gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg catatacaga 6420
gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt 6480
ctagatcgga gtagaatact gtttcaaact acctggtgta tttattaatt ttggaactgt 6540
atgtgtgtgt catacatctt catagttacg agtttaagat ggatggaaat atcgatctag 6600
gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca 6660
tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata 6720
attattttga tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt 6780
ttagccctgc cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac 6840
cctgttgttt ggtgttactt ctgcagggat ctccgatcat gcaaaaactc attaactcag 6900
tgcaaaacta tgcctggggc agcaaaacgg cgttgactga actttatggt atggaaaatc 6960
cgtccagcca gccgatggcc gagctgtgga tgggcgcaca tccgaaaagc agttcacgag 7020
tgcagaatgc cgccggagat atcgtttcac tgcgtgatgt gattgagagt gataaatcga 7080
ctctgctcgg agaggccgtt gccaaacgct ttggcgaact gcctttcctg ttcaaagtat 7140
tatgcgcagc acagccactc tccattcagg ttcatccaaa caaacacaat tctgaaatcg 7200
gttttgccaa agaaaatgcc gcaggtatcc cgatggatgc cgccgagcgt aactataaag 7260
atcctaacca caagccggag ctggtttttg cgctgacgcc tttccttgcg atgaacgcgt 7320
ttcgtgaatt ttccgagatt gtctccctac tccagccggt cgcaggtgca catccggcga 7380
ttgctcactt tttacaacag cctgatgccg aacgtttaag cgaactgttc gccagcctgt 7440
tgaatatgca gggtgaagaa aaatcccgcg cgctggcgat tttaaaatcg gccctcgata 7500
gccagcaggg tgaaccgtgg caaacgattc gtttaatttc tgaattttac ccggaagaca 7560
gcggtctgtt ctccccgcta ttgctgaatg tggtgaaatt gaaccctggc gaagcgatgt 7620
tcctgttcgc tgaaacaccg cacgcttacc tgcaaggcgt ggcgctggaa gtgatggcaa 7680
actccgataa cgtgctgcgt gcgggtctga cgcctaaata cattgatatt ccggaactgg 7740
ttgccaatgt gaaattcgaa gccaaaccgg ctaaccagtt gttgacccag ccggtgaaac 7800
aaggtgcaga actggacttc ccgattccag tggatgattt tgccttctcg ctgcatgacc 7860
ttagtgataa agaaaccacc attagccagc agagtgccgc cattttgttc tgcgtcgaag 7920
gcgatgcaac gttgtggaaa ggttctcagc agttacagct taaaccgggt gaatcagcgt 7980
ttattgccgc caacgaatca ccggtgactg tcaaaggcca cggccgttta gcgcgtgttt 8040
acaacaagct gtaagagctt actgaaaaaa ttaacatctc ttgctaagct gggtcatggg 8100
tcgtttaagc tgccgatgtg cctgcgtcgt ctggtgccct ctctccatat ggaggttgtc 8160
aaagtatctg ctgttcgtgt catgagtcgt gtcagtgttg gtttaataat ggaccggttg 8220
tgttgtgtgt gcgtactacc cagaactatg acaaatcatg aataagtttg atgtttgaaa 8280
ttaaagcctg tgctcattat gttctgtctt tcagttgtct cctaatattt gcctccaggt 8340
actggctatc taccgtttct tacttaggag gtgtttgaat gcactaaaac taatagttag 8400
tggctaaaat tagttaaaac atccaaacac catagctaat agttgaacta ttagctattt 8460
ttggaaaatt agttaatagt gaggtagtta tttgttagct agctaattca actaacaatt 8520
tttagccaac taacaattag tttcagtgca ttcaaacacc cccttaatgt taacgtggtt 8580
ctatctaccg tctcctaata tatggttgat tgttcggttt gttgctatgc tattgggttc 8640
tgattgctgc tagttcttgc tgaatccaga agttctcgta gtatagctca gattcatatt 8700
atttatttga gtgataagtg atccaggtta ttactatgtt agctaggttt tttttacaag 8760
gataaattat ctgtgatcat aattcttatg aaagctttat gtttcctgga ggcagtggca 8820
tgcaatgcat gacagcaact tgatcacacc agctgaggta gatacggtaa caaggttctt 8880
aaatctgttc accaaatcat tggagaacac acatacacat tcttgccagt cttggttaga 8940
gaaatttcat gacaaaatgc caaagctgtc ttgactcttc acttttggcc atgagtcgtg 9000
acttagtttg gtttaatgga ccggttctcc tagcttgttc tactcaaaac tgttgttgat 9060
gcgaataagt tgtgatggtt gatctctgga ttttgttttg ctctcaatag tggacgagat 9120
tagatagcct gcaggcccgg gggcgcgccc taattagcta acggccagga tcgccgcgtg 9180
agcctttagc aactagctag attaattaac gcaatctgtt attaagttgt ctaagcgtca 9240
atttgtttac accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc 9300
tcggcacaaa atcaccactc gatacaggca gcccatcaga attaattctc atgtttgaca 9360
gcttatcatc gactgcacgg tgcaccaatg cttctggcgt caggcagcca tcggaagctg 9420
tggtatggct gtgcaggtcg taaatcactg cataattcgt gtcgctcaag gcgcactccc 9480
gttctggata atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga 9540
gctgttgaca attaatcatc cggctcgtat aatgtgtgga attgtgagcg gataacaatt 9600
tcacacagga aacagaccat gagggaagcg ttgatcgccg aagtatcgac tcaactatca 9660
gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac 9720
ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg 9780
accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg 9840
gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac 9900
gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc 9960
aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg 10020
ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt 10080
gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac 10140
tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg 10200
tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag 10260
cgcctgccgg cccagtatca gcccgtcata cttgaagcta ggcaggctta tcttggacaa 10320
gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgttcacta cgtgaaaggc 10380
gagatcacca aagtagtcgg caaataaagc tctagtggat ctccgtaccc agggatctgg 10440
ctcgcggcgg acgcacgacg ccggggcgag accataggcg atctcctaaa tcaatagtag 10500
ctgtaacctc gaagcgtttc acttgtaaca acgattgaga atttttgtca taaaattgaa 10560
atacttggtt cgcatttttg tcatccgcgg tcagccgcaa ttctgacgaa ctgcccattt 10620
agctggagat gattgtacat ccttcacgtg aaaatttctc aagcgctgtg aacaagggtt 10680
cagattttag attgaaaggt gagccgttga aacacgttct tcttgtcgat gacgacgtcg 10740
ctatgcggca tcttattatt gaatacctta cgatccacgc cttcaaagtg accgcggtag 10800
ccgacagcac ccagttcaca agagtactct cttccgcgac ggtcgatgtc gtggttgttg 10860
atctagattt aggtcgtgaa gatgggctcg agatcgttcg taatctggcg gcaaagtctg 10920
atattccaat cataattatc agtggcgacc gccttgagga gacggataaa gttgttgcac 10980
tcgagctagg agcaagtgat tttatcgcta agccgttcag tatcagagag tttctagcac 11040
gcattcgggt tgccttgcgc gtgcgcccca acgttgtccg ctccaaagac cgacggtctt 11100
tttgttttac tgactggaca cttaatctca ggcaacgtcg cttgatgtcc gaagctggcg 11160
gtgaggtgaa acttacggca ggtgagttca atcttctcct cgcgttttta gagaaacccc 11220
gcgacgttct atcgcgcgag caacttctca ttgccagtcg agtacgcgac gaggaggttt 11280
atgacaggag tatagatgtt ctcattttga ggctgcgccg caaacttgag gcagatccgt 11340
caagccctca actgataaaa acagcaagag gtgccggtta tttctttgac gcggacgtgc 11400
aggtttcgca cggggggacg atggcagcct gagccaattc ccagatcccc gaggaatcgg 11460
cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct 11520
ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg 11580
ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc 11640
ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt 11700
cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt 11760
tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga 11820
cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct 11880
ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg 11940
agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg 12000
agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac 12060
gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga 12120
gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta 12180
catcgagatc gagctggctg attggatgta ccgcgagatc acagaaggca agaacccgga 12240
cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta 12300
ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta 12360
cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat 12420
cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat 12480
cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac 12540
ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc 12600
tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat 12660
tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga 12720
gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg 12780
cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac 12840
ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg 12900
ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc 12960
gtcgccactc gaccgccggc gctgaggtct gcctcgtgaa gaaggtgttg ctgactcata 13020
ccaggcctga atcgccccat catccagcca gaaagtgagg gagccacggt tgatgagagc 13080
tttgttgtag gtggaccagt tggtgatttt gaacttttgc tttgccacgg aacggtctgc 13140
gttgtcggga agatgcgtga tctgatcctt caactcagca aaagttcgat ttattcaaca 13200
aagccgccgt cccgtcaagt cagcgtaatg ctctgccagt gttacaacca attaaccaat 13260
tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta 13320
tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag 13380
ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata 13440
caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg 13500
acgactgaat ccggtgagaa tggcaaaagc tctgcattaa tgaatcggcc aacgcgcggg 13560
gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 13620
ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 13680
agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 13740
ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 13800
caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 13860
gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 13920
cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 13980
tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 14040
gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 14100
cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 14160
tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 14220
tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 14280
caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 14340
aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 14400
cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 14460
ccttttgatc cggaatta 14478
<210> 113
<211> 14466
<212> DNA
<213> 人工序列
<220>
<223> 23718载体
<400> 113
attcctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt 60
taaatatccg attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc 120
tgtcaaacac tgatagttta aactggcact agcctaacgg tgttgactaa ctaggccgct 180
tccctaatta gctaacccgg gggcgcgccg ggaccctaat tagctaaggt acctgcagtg 240
cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa 300
aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata 360
catatattta aactttactc tacgaataat ataatctata gtactacaat aatatcagtg 420
ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg 480
acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa 540
atagcttcac ctatataata cttcatccat tttattagta catccattta gggtttaggg 600
ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta 660
aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga tataaaatag 720
aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg 780
aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta 840
acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg 900
catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc 960
cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg 1020
gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc 1080
gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa 1140
cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg 1200
cacctccgct tcctaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta 1260
gatcggcgtt ccggtccata gttagggccc ggtagttcta cttctgttca tgtttgtgtt 1320
agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg 1380
tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct 1440
ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt 1500
ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt 1560
catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg 1620
gagtagaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt 1680
gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag 1740
gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg 1800
ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact 1860
gtttcaaact acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt 1920
catagttacg agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg 1980
tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc 2040
ttgagtacct atctattata ataaacaagt atgttttata attattttga tcttgatata 2100
cttggatgat ggcatatcca gcagctatat gtggattttt ttagccctgc cttcatacgc 2160
tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt 2220
ctgcaggatc ctctaagtag cagccatgaa caagaacaac accaagctga gcacccgcgc 2280
cctgccgagc ttcatcgact acttcaacgg catctacggc ttcgccaccg gcatcaagga 2340
catcatgaac atgatcttca agaccgacac cggcggcgac ctgaccctgg acgagatcct 2400
gaagaaccag cagctgctga acgacatcag cggcaagctg gacggcgtga acggcagcct 2460
gaacgacctg atcgcccagg gcaacctgaa caccgagctg agcaaggaga tccttaagat 2520
cgccaacgag cagaaccagg tgctgaacga cgtgaacaac aagctggacg ccatcaacac 2580
catgctgcgc gtgtacctgc cgaagatcac cagcatgctg agcgacgtga ttaagcagaa 2640
ctacgccctg agcctgcaga tcgagtacct gagcaagcag ctgcaggaga tcagcgacaa 2700
gctggacatc atcaacgtga acgtcctgat caacagcacc ctgaccgaga tcaccccggc 2760
ctaccagcgc atcaagtacg tgaacgagaa gttcgaagag ctgaccttcg ccaccgagac 2820
cagcagcgct gagatattag aggaaactga agacggcagc ccggccgaca tcctggacga 2880
gctgaccgag ctgaccgagc tggcgaagag cgtgaccaag aacgacgtgg acggcttcga 2940
gttctacctg aacaccttcc acgacgtgat ggtgggcaac aacctgttcg gccgcagcgc 3000
cctgaagacc gccagcgagc tgatcaccaa ggagaacgtg aagaccagcg gcagcgaggt 3060
gggcaacgtg tacaacttcc tgatcgtgct gaccgccctg caggcccagg ccttcctgac 3120
cctgaccacc tgtcgcaagc tgctgggcct ggccgacatc gactacacca gcatcatgaa 3180
cgagcacttg aacaaggaga aggaggagtt ccgcgtgaac atcctgccga ccctgagcaa 3240
caccttcagc aacccgaact acgccaaggt gaagggcagc gacgaggacg ccaagatgat 3300
cgtggaggct aagccgggcc acgcgttgat cggcttcgag atcagcaacg acagcatcac 3360
cgtgctgaag gtgtacgagg ccaagctgaa gcagaactac caggtggaca aggacagctt 3420
gagcgaggtg atctacggcg acatggacaa gctgctgtgt ccggaccaga gcgagcaaat 3480
ctactacacc aacaacatcg tgttcccgaa cgagtacgtg atcaccaaga tcgacttcac 3540
caagaagatg aagaccctgc gctacgaggt gaccgccaac ttctacgaca gcagcaccgg 3600
cgagatcgac ctgaacaaga agaaggtgga gagcagcgag gccgagtacc gcaccctgag 3660
cgcgaacgac gacggcgtct acatgccact gggcgtgatc agcgagacct tcctgacccc 3720
gatcaacggc tttggcctgc aggccgacga gaacagccgc ctgatcaccc tgacctgtaa 3780
gagctacctg cgcgagctgc tgctagccac cgacctgagc aacaaggaga ccaagctgat 3840
cgtgccaccg agcggcttca tcagcaacat cgtggagaac ggcagcatcg aggaggacaa 3900
cctggagccg tggaaggcca acaacaagaa cgcctacgtc gaccacaccg gcggcgtgaa 3960
cggcaccaag gccctgtacg tgcacaagga cggcggcatc agccagttca tcggcgacaa 4020
gctgaagccg aagaccgagt acgtgatcca gtacaccgtg aagggcaagc catcgattca 4080
cctgaaggac gagaacaccg gctacatcca ctacgaggac accaacaaca acctggagga 4140
ctaccagacc atcaacaagc gcttcaccac cggcaccgac ctgaagggcg tgtacctgat 4200
cctgaagagc cagaacggcg acgaggcctg gggcgacaac ttcatcatcc tggagatcag 4260
cccgagcgag aagctgctga gcccggagct gatcaacacc aacaactgga ccagcaccgg 4320
cagcaccaac atcagcggca acaccctgac cctgtaccag ggcggccgcg gcatcctgaa 4380
gcagaacctg cagctggaca gcttcagcac ctaccgcgtg tacttcagcg tgagcggcga 4440
cgccaacgtg cgcatccgca actcccgcga ggtgctgttc gagaagaggt acatgagcgg 4500
cgccaaggac gtgagcgaga tgttcaccac caagttcgag aaggacaact tctacatcga 4560
gctgagccag ggcaacaacc tgtacggcgg cccgatcgtg cacttctacg acgtgagcat 4620
caagtaggag ctctagatct tatctgcaca aagtggagta gtcagtcatc gatcaggaac 4680
cagacaccag acttttattc atacagtgaa gtgaagtgaa gtgcagtgca gtgagttgct 4740
ggtttttgta caacttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc 4800
taattcctaa aaccaaaatc caggctacac ggaccgcgat cgcttaatta agcttgcatg 4860
cctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct 4920
aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct 4980
atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata 5040
atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg 5100
agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt 5160
tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag 5220
ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt 5280
tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat 5340
ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa 5400
aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgccg 5460
acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag 5520
acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg 5580
gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca 5640
cggcaggcgg cctcctcctc ctctcacggc accggcagct acgggggatt cctttcccac 5700
cgctccttcg ctttcccttc ctcgcccgcc gtaataaata gacaccccct ccacaccctc 5760
tttccccaac ctcgtgttgt tcggagcgca cacacacaca accagatctc ccccaaatcc 5820
acccgtcggc acctccgctt caaggtacgc cgctcgtcct cccccccccc ccctctctac 5880
cttctctaga tcggcgttcc ggtccatagt tagggcccgg tagttctact tctgttcatg 5940
tttgtgttag atccgtgttt gtgttagatc cgtgctgtta gcgttcgtac acggatgcga 6000
cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg 6060
ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca 6120
tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt 6180
catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt 6240
ctagatcgga gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt 6300
atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc 6360
taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt 6420
tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt 6480
agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca 6540
tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca 6600
tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg 6660
ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tattttgatc 6720
ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt agccctgcct 6780
tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg 6840
tgttacttct gcagggatct ccgatcatgc aaaaactcat taactcagtg caaaactatg 6900
cctggggcag caaaacggcg ttgactgaac tttatggtat ggaaaatccg tccagccagc 6960
cgatggccga gctgtggatg ggcgcacatc cgaaaagcag ttcacgagtg cagaatgccg 7020
ccggagatat cgtttcactg cgtgatgtga ttgagagtga taaatcgact ctgctcggag 7080
aggccgttgc caaacgcttt ggcgaactgc ctttcctgtt caaagtatta tgcgcagcac 7140
agccactctc cattcaggtt catccaaaca aacacaattc tgaaatcggt tttgccaaag 7200
aaaatgccgc aggtatcccg atggatgccg ccgagcgtaa ctataaagat cctaaccaca 7260
agccggagct ggtttttgcg ctgacgcctt tccttgcgat gaacgcgttt cgtgaatttt 7320
ccgagattgt ctccctactc cagccggtcg caggtgcaca tccggcgatt gctcactttt 7380
tacaacagcc tgatgccgaa cgtttaagcg aactgttcgc cagcctgttg aatatgcagg 7440
gtgaagaaaa atcccgcgcg ctggcgattt taaaatcggc cctcgatagc cagcagggtg 7500
aaccgtggca aacgattcgt ttaatttctg aattttaccc ggaagacagc ggtctgttct 7560
ccccgctatt gctgaatgtg gtgaaattga accctggcga agcgatgttc ctgttcgctg 7620
aaacaccgca cgcttacctg caaggcgtgg cgctggaagt gatggcaaac tccgataacg 7680
tgctgcgtgc gggtctgacg cctaaataca ttgatattcc ggaactggtt gccaatgtga 7740
aattcgaagc caaaccggct aaccagttgt tgacccagcc ggtgaaacaa ggtgcagaac 7800
tggacttccc gattccagtg gatgattttg ccttctcgct gcatgacctt agtgataaag 7860
aaaccaccat tagccagcag agtgccgcca ttttgttctg cgtcgaaggc gatgcaacgt 7920
tgtggaaagg ttctcagcag ttacagctta aaccgggtga atcagcgttt attgccgcca 7980
acgaatcacc ggtgactgtc aaaggccacg gccgtttagc gcgtgtttac aacaagctgt 8040
aagagcttac tgaaaaaatt aacatctctt gctaagctgg gtcatgggtc gtttaagctg 8100
ccgatgtgcc tgcgtcgtct ggtgccctct ctccatatgg aggttgtcaa agtatctgct 8160
gttcgtgtca tgagtcgtgt cagtgttggt ttaataatgg accggttgtg ttgtgtgtgc 8220
gtactaccca gaactatgac aaatcatgaa taagtttgat gtttgaaatt aaagcctgtg 8280
ctcattatgt tctgtctttc agttgtctcc taatatttgc ctccaggtac tggctatcta 8340
ccgtttctta cttaggaggt gtttgaatgc actaaaacta atagttagtg gctaaaatta 8400
gttaaaacat ccaaacacca tagctaatag ttgaactatt agctattttt ggaaaattag 8460
ttaatagtga ggtagttatt tgttagctag ctaattcaac taacaatttt tagccaacta 8520
acaattagtt tcagtgcatt caaacacccc cttaatgtta acgtggttct atctaccgtc 8580
tcctaatata tggttgattg ttcggtttgt tgctatgcta ttgggttctg attgctgcta 8640
gttcttgctg aatccagaag ttctcgtagt atagctcaga ttcatattat ttatttgagt 8700
gataagtgat ccaggttatt actatgttag ctaggttttt tttacaagga taaattatct 8760
gtgatcataa ttcttatgaa agctttatgt ttcctggagg cagtggcatg caatgcatga 8820
cagcaacttg atcacaccag ctgaggtaga tacggtaaca aggttcttaa atctgttcac 8880
caaatcattg gagaacacac atacacattc ttgccagtct tggttagaga aatttcatga 8940
caaaatgcca aagctgtctt gactcttcac ttttggccat gagtcgtgac ttagtttggt 9000
ttaatggacc ggttctccta gcttgttcta ctcaaaactg ttgttgatgc gaataagttg 9060
tgatggttga tctctggatt ttgttttgct ctcaatagtg gacgagatta gatagcctgc 9120
aggcccgggg gcgcgcccta attagctaac ggccaggatc gccgcgtgag cctttagcaa 9180
ctagctagat taattaacgc aatctgttat taagttgtct aagcgtcaat ttgtttacac 9240
cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat 9300
caccactcga tacaggcagc ccatcagaat taattctcat gtttgacagc ttatcatcga 9360
ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg gtatggctgt 9420
gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt tctggataat 9480
gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat 9540
taatcatccg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 9600
cagaccatga gggaagcgtt gatcgccgaa gtatcgactc aactatcaga ggtagttggc 9660
gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg 9720
gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt 9780
gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga 9840
gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg 9900
tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt 9960
gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca 10020
agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct 10080
gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac 10140
tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta 10200
accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc 10260
cagtatcagc ccgtcatact tgaagctagg caggcttatc ttggacaaga agatcgcttg 10320
gcctcgcgcg cagatcagtt ggaagaattt gttcactacg tgaaaggcga gatcaccaaa 10380
gtagtcggca aataaagctc tagtggatct ccgtacccag ggatctggct cgcggcggac 10440
gcacgacgcc ggggcgagac cataggcgat ctcctaaatc aatagtagct gtaacctcga 10500
agcgtttcac ttgtaacaac gattgagaat ttttgtcata aaattgaaat acttggttcg 10560
catttttgtc atccgcggtc agccgcaatt ctgacgaact gcccatttag ctggagatga 10620
ttgtacatcc ttcacgtgaa aatttctcaa gcgctgtgaa caagggttca gattttagat 10680
tgaaaggtga gccgttgaaa cacgttcttc ttgtcgatga cgacgtcgct atgcggcatc 10740
ttattattga ataccttacg atccacgcct tcaaagtgac cgcggtagcc gacagcaccc 10800
agttcacaag agtactctct tccgcgacgg tcgatgtcgt ggttgttgat ctagatttag 10860
gtcgtgaaga tgggctcgag atcgttcgta atctggcggc aaagtctgat attccaatca 10920
taattatcag tggcgaccgc cttgaggaga cggataaagt tgttgcactc gagctaggag 10980
caagtgattt tatcgctaag ccgttcagta tcagagagtt tctagcacgc attcgggttg 11040
ccttgcgcgt gcgccccaac gttgtccgct ccaaagaccg acggtctttt tgttttactg 11100
actggacact taatctcagg caacgtcgct tgatgtccga agctggcggt gaggtgaaac 11160
ttacggcagg tgagttcaat cttctcctcg cgtttttaga gaaaccccgc gacgttctat 11220
cgcgcgagca acttctcatt gccagtcgag tacgcgacga ggaggtttat gacaggagta 11280
tagatgttct cattttgagg ctgcgccgca aacttgaggc agatccgtca agccctcaac 11340
tgataaaaac agcaagaggt gccggttatt tctttgacgc ggacgtgcag gtttcgcacg 11400
gggggacgat ggcagcctga gccaattccc agatccccga ggaatcggcg tgagcggtcg 11460
caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt 11520
gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc 11580
gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc 11640
gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct 11700
ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc 11760
gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga 11820
ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc 11880
ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg 11940
ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg 12000
aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat 12060
gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt 12120
gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga 12180
gctggctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt 12240
tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg 12300
ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg 12360
cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga 12420
cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg 12480
ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct 12540
agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac 12600
gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa 12660
gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga 12720
tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc 12780
ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct 12840
gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat 12900
ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga 12960
ccgccggcgc tgaggtctgc ctcgtgaaga aggtgttgct gactcatacc aggcctgaat 13020
cgccccatca tccagccaga aagtgaggga gccacggttg atgagagctt tgttgtaggt 13080
ggaccagttg gtgattttga acttttgctt tgccacggaa cggtctgcgt tgtcgggaag 13140
atgcgtgatc tgatccttca actcagcaaa agttcgattt attcaacaaa gccgccgtcc 13200
cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat taaccaattc tgattagaaa 13260
aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat 13320
ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg 13380
gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat 13440
ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc 13500
ggtgagaatg gcaaaagctc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 13560
gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 13620
gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 13680
taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 13740
cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 13800
ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 13860
aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 13920
tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 13980
gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 14040
cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 14100
ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 14160
cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 14220
gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 14280
cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 14340
tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 14400
ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttgatccg 14460
gaatta 14466
<210> 114
<211> 789
<212> PRT
<213> 人工序列
<220>
<223> 23705 Vip3
<400> 114
Met Asn Lys Asn Asn Thr Lys Leu Ser Thr Arg Ala Leu Pro Ser Phe
1 5 10 15
Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys Asp
20 25 30
Ile Met Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asp Leu Thr Leu
35 40 45
Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn Asp Ile Ser Gly Lys
50 55 60
Leu Asp Gly Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln Gly Asn
65 70 75 80
Leu Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn Glu Gln
85 90 95
Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile Asn Thr
100 105 110
Met Leu Arg Val Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser Asp Val
115 120 125
Ile Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr Leu Ser Lys
130 135 140
Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Ile Ile Asn Val Asn Val
145 150 155 160
Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ala Tyr Gln Arg Ile
165 170 175
Lys Tyr Val Asn Glu Lys Phe Glu Glu Leu Thr Phe Ala Thr Glu Thr
180 185 190
Ser Ser Lys Val Lys Lys Asp Gly Ser Pro Ala Asp Ile Leu Asp Glu
195 200 205
Leu Thr Glu Leu Thr Glu Leu Ala Lys Ser Val Thr Lys Asn Asp Val
210 215 220
Asp Gly Phe Glu Phe Tyr Leu Asn Thr Phe His Asp Val Met Val Gly
225 230 235 240
Asn Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr Ala Ser Glu Leu Ile
245 250 255
Thr Lys Glu Asn Val Lys Thr Ser Gly Ser Glu Val Gly Asn Val Tyr
260 265 270
Asn Phe Leu Ile Val Leu Thr Ala Leu Gln Ala Gln Ala Phe Leu Thr
275 280 285
Leu Thr Thr Cys Arg Lys Leu Leu Gly Leu Ala Asp Ile Asp Tyr Thr
290 295 300
Ser Ile Met Asn Glu His Leu Asn Lys Glu Lys Glu Glu Phe Arg Val
305 310 315 320
Asn Ile Leu Pro Thr Leu Ser Asn Thr Phe Ser Asn Pro Asn Tyr Ala
325 330 335
Lys Val Lys Gly Ser Asp Glu Asp Ala Lys Met Ile Val Glu Ala Lys
340 345 350
Pro Gly His Ala Leu Ile Gly Phe Glu Ile Ser Asn Asp Ser Ile Thr
355 360 365
Val Leu Lys Val Tyr Glu Ala Lys Leu Lys Gln Asn Tyr Gln Val Asp
370 375 380
Lys Asp Ser Leu Ser Glu Val Ile Tyr Gly Asp Met Asp Lys Leu Leu
385 390 395 400
Cys Pro Asp Gln Ser Glu Gln Ile Tyr Tyr Thr Asn Asn Ile Val Phe
405 410 415
Pro Asn Glu Tyr Val Ile Thr Lys Ile Asp Phe Thr Lys Lys Met Lys
420 425 430
Thr Leu Arg Tyr Glu Val Thr Ala Asn Phe Tyr Asp Ser Ser Thr Gly
435 440 445
Glu Ile Asp Leu Asn Lys Lys Lys Val Glu Ser Ser Glu Ala Glu Tyr
450 455 460
Arg Thr Leu Ser Ala Asn Asp Asp Gly Val Tyr Met Pro Leu Gly Val
465 470 475 480
Ile Ser Glu Thr Phe Leu Thr Pro Ile Asn Gly Phe Gly Leu Gln Ala
485 490 495
Asp Glu Asn Ser Arg Leu Ile Thr Leu Thr Cys Lys Ser Tyr Leu Arg
500 505 510
Glu Leu Leu Leu Ala Thr Asp Leu Ser Asn Lys Glu Thr Lys Leu Ile
515 520 525
Val Pro Pro Ser Gly Phe Ile Ser Asn Ile Val Glu Asn Gly Ser Ile
530 535 540
Glu Glu Asp Asn Leu Glu Pro Trp Lys Ala Asn Asn Lys Asn Ala Tyr
545 550 555 560
Val Asp His Thr Gly Gly Val Asn Gly Thr Lys Ala Leu Tyr Val His
565 570 575
Lys Asp Gly Gly Ile Ser Gln Phe Ile Gly Asp Lys Leu Lys Pro Lys
580 585 590
Thr Glu Tyr Val Ile Gln Tyr Thr Val Lys Gly Lys Pro Ser Ile His
595 600 605
Leu Lys Asp Glu Asn Thr Gly Tyr Ile His Tyr Glu Asp Thr Asn Asn
610 615 620
Asn Leu Glu Asp Tyr Gln Thr Ile Asn Lys Arg Phe Thr Thr Gly Thr
625 630 635 640
Asp Leu Lys Gly Val Tyr Leu Ile Leu Lys Ser Gln Asn Gly Asp Glu
645 650 655
Ala Trp Gly Asp Asn Phe Ile Ile Leu Glu Ile Ser Pro Ser Glu Lys
660 665 670
Leu Leu Ser Pro Glu Leu Ile Asn Thr Asn Asn Trp Thr Ser Thr Gly
675 680 685
Ser Thr Asn Ile Ser Gly Asn Thr Leu Thr Leu Tyr Gln Gly Gly Arg
690 695 700
Gly Ile Leu Lys Gln Asn Leu Gln Leu Asp Ser Phe Ser Thr Tyr Arg
705 710 715 720
Val Tyr Phe Ser Val Ser Gly Asp Ala Asn Val Arg Ile Arg Asn Ser
725 730 735
Arg Glu Val Leu Phe Glu Lys Arg Tyr Met Ser Gly Ala Lys Asp Val
740 745 750
Ser Glu Met Phe Thr Thr Lys Phe Glu Lys Asp Asn Phe Tyr Ile Glu
755 760 765
Leu Ser Gln Gly Asn Asn Leu Tyr Gly Gly Pro Ile Val His Phe Tyr
770 775 780
Asp Val Ser Ile Lys
785
<210> 115
<211> 807
<212> PRT
<213> 人工序列
<220>
<223> 23716 Vip3
<400> 115
Met Tyr Thr His Thr His Val Glu Ile Leu Glu Glu Thr Glu Glu Leu
1 5 10 15
Arg His Ile Asn Lys Asn Asn Thr Lys Leu Ser Thr Arg Ala Leu Pro
20 25 30
Ser Phe Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile
35 40 45
Lys Asp Ile Met Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asp Leu
50 55 60
Thr Leu Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn Asp Ile Ser
65 70 75 80
Gly Lys Leu Asp Gly Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln
85 90 95
Gly Asn Leu Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn
100 105 110
Glu Gln Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile
115 120 125
Asn Thr Met Leu Arg Val Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser
130 135 140
Asp Val Ile Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr Leu
145 150 155 160
Ser Lys Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Ile Ile Asn Val
165 170 175
Asn Val Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ala Tyr Gln
180 185 190
Arg Ile Lys Tyr Val Asn Glu Lys Phe Glu Glu Leu Thr Phe Ala Thr
195 200 205
Glu Thr Ser Ser Lys Val Lys Lys Asp Gly Ser Pro Ala Asp Ile Leu
210 215 220
Asp Glu Leu Thr Glu Leu Thr Glu Leu Ala Lys Ser Val Thr Lys Asn
225 230 235 240
Asp Val Asp Gly Phe Glu Phe Tyr Leu Asn Thr Phe His Asp Val Met
245 250 255
Val Gly Asn Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr Ala Ser Glu
260 265 270
Leu Ile Thr Lys Glu Asn Val Lys Thr Ser Gly Ser Glu Val Gly Asn
275 280 285
Val Tyr Asn Phe Leu Ile Val Leu Thr Ala Leu Gln Ala Gln Ala Phe
290 295 300
Leu Thr Leu Thr Thr Cys Arg Lys Leu Leu Gly Leu Ala Asp Ile Asp
305 310 315 320
Tyr Thr Ser Ile Met Asn Glu His Leu Asn Lys Glu Lys Glu Glu Phe
325 330 335
Arg Val Asn Ile Leu Pro Thr Leu Ser Asn Thr Phe Ser Asn Pro Asn
340 345 350
Tyr Ala Lys Val Lys Gly Ser Asp Glu Asp Ala Lys Met Ile Val Glu
355 360 365
Ala Lys Pro Gly His Ala Leu Ile Gly Phe Glu Ile Ser Asn Asp Ser
370 375 380
Ile Thr Val Leu Lys Val Tyr Glu Ala Lys Leu Lys Gln Asn Tyr Gln
385 390 395 400
Val Asp Lys Asp Ser Leu Ser Glu Val Ile Tyr Gly Asp Met Asp Lys
405 410 415
Leu Leu Cys Pro Asp Gln Ser Glu Gln Ile Tyr Tyr Thr Asn Asn Ile
420 425 430
Val Phe Pro Asn Glu Tyr Val Ile Thr Lys Ile Asp Phe Thr Lys Lys
435 440 445
Met Lys Thr Leu Arg Tyr Glu Val Thr Ala Asn Phe Tyr Asp Ser Ser
450 455 460
Thr Gly Glu Ile Asp Leu Asn Lys Lys Lys Val Glu Ser Ser Glu Ala
465 470 475 480
Glu Tyr Arg Thr Leu Ser Ala Asn Asp Asp Gly Val Tyr Met Pro Leu
485 490 495
Gly Val Ile Ser Glu Thr Phe Leu Thr Pro Ile Asn Gly Phe Gly Leu
500 505 510
Gln Ala Asp Glu Asn Ser Arg Leu Ile Thr Leu Thr Cys Lys Ser Tyr
515 520 525
Leu Arg Glu Leu Leu Leu Ala Thr Asp Leu Ser Asn Lys Glu Thr Lys
530 535 540
Leu Ile Val Pro Pro Ser Gly Phe Ile Ser Asn Ile Val Glu Asn Gly
545 550 555 560
Ser Ile Glu Glu Asp Asn Leu Glu Pro Trp Lys Ala Asn Asn Lys Asn
565 570 575
Ala Tyr Val Asp His Thr Gly Gly Val Asn Gly Thr Lys Ala Leu Tyr
580 585 590
Val His Lys Asp Gly Gly Ile Ser Gln Phe Ile Gly Asp Lys Leu Lys
595 600 605
Pro Lys Thr Glu Tyr Val Ile Gln Tyr Thr Val Lys Gly Lys Pro Ser
610 615 620
Ile His Leu Lys Asp Glu Asn Thr Gly Tyr Ile His Tyr Glu Asp Thr
625 630 635 640
Asn Asn Asn Leu Glu Asp Tyr Gln Thr Ile Asn Lys Arg Phe Thr Thr
645 650 655
Gly Thr Asp Leu Lys Gly Val Tyr Leu Ile Leu Lys Ser Gln Asn Gly
660 665 670
Asp Glu Ala Trp Gly Asp Asn Phe Ile Ile Leu Glu Ile Ser Pro Ser
675 680 685
Glu Lys Leu Leu Ser Pro Glu Leu Ile Asn Thr Asn Asn Trp Thr Ser
690 695 700
Thr Gly Ser Thr Asn Ile Ser Gly Asn Thr Leu Thr Leu Tyr Gln Gly
705 710 715 720
Gly Arg Gly Ile Leu Lys Gln Asn Leu Gln Leu Asp Ser Phe Ser Thr
725 730 735
Tyr Arg Val Tyr Phe Ser Val Ser Gly Asp Ala Asn Val Arg Ile Arg
740 745 750
Asn Ser Arg Glu Val Leu Phe Glu Lys Arg Tyr Met Ser Gly Ala Lys
755 760 765
Asp Val Ser Glu Met Phe Thr Thr Lys Phe Glu Lys Asp Asn Phe Tyr
770 775 780
Ile Glu Leu Ser Gln Gly Asn Asn Leu Tyr Gly Gly Pro Ile Val His
785 790 795 800
Phe Tyr Asp Val Ser Ile Lys
805
<210> 116
<211> 797
<212> PRT
<213> 人工序列
<220>
<223> 23717 Vip3
<400> 116
Met Ala Glu Ile Leu Glu Glu Thr Glu Asn Lys Asn Asn Thr Lys Leu
1 5 10 15
Ser Thr Arg Ala Leu Pro Ser Phe Ile Asp Tyr Phe Asn Gly Ile Tyr
20 25 30
Gly Phe Ala Thr Gly Ile Lys Asp Ile Met Asn Met Ile Phe Lys Thr
35 40 45
Asp Thr Gly Gly Asp Leu Thr Leu Asp Glu Ile Leu Lys Asn Gln Gln
50 55 60
Leu Leu Asn Asp Ile Ser Gly Lys Leu Asp Gly Val Asn Gly Ser Leu
65 70 75 80
Asn Asp Leu Ile Ala Gln Gly Asn Leu Asn Thr Glu Leu Ser Lys Glu
85 90 95
Ile Leu Lys Ile Ala Asn Glu Gln Asn Gln Val Leu Asn Asp Val Asn
100 105 110
Asn Lys Leu Asp Ala Ile Asn Thr Met Leu Arg Val Tyr Leu Pro Lys
115 120 125
Ile Thr Ser Met Leu Ser Asp Val Ile Lys Gln Asn Tyr Ala Leu Ser
130 135 140
Leu Gln Ile Glu Tyr Leu Ser Lys Gln Leu Gln Glu Ile Ser Asp Lys
145 150 155 160
Leu Asp Ile Ile Asn Val Asn Val Leu Ile Asn Ser Thr Leu Thr Glu
165 170 175
Ile Thr Pro Ala Tyr Gln Arg Ile Lys Tyr Val Asn Glu Lys Phe Glu
180 185 190
Glu Leu Thr Phe Ala Thr Glu Thr Ser Ser Lys Val Lys Lys Asp Gly
195 200 205
Ser Pro Ala Asp Ile Leu Asp Glu Leu Thr Glu Leu Thr Glu Leu Ala
210 215 220
Lys Ser Val Thr Lys Asn Asp Val Asp Gly Phe Glu Phe Tyr Leu Asn
225 230 235 240
Thr Phe His Asp Val Met Val Gly Asn Asn Leu Phe Gly Arg Ser Ala
245 250 255
Leu Lys Thr Ala Ser Glu Leu Ile Thr Lys Glu Asn Val Lys Thr Ser
260 265 270
Gly Ser Glu Val Gly Asn Val Tyr Asn Phe Leu Ile Val Leu Thr Ala
275 280 285
Leu Gln Ala Gln Ala Phe Leu Thr Leu Thr Thr Cys Arg Lys Leu Leu
290 295 300
Gly Leu Ala Asp Ile Asp Tyr Thr Ser Ile Met Asn Glu His Leu Asn
305 310 315 320
Lys Glu Lys Glu Glu Phe Arg Val Asn Ile Leu Pro Thr Leu Ser Asn
325 330 335
Thr Phe Ser Asn Pro Asn Tyr Ala Lys Val Lys Gly Ser Asp Glu Asp
340 345 350
Ala Lys Met Ile Val Glu Ala Lys Pro Gly His Ala Leu Ile Gly Phe
355 360 365
Glu Ile Ser Asn Asp Ser Ile Thr Val Leu Lys Val Tyr Glu Ala Lys
370 375 380
Leu Lys Gln Asn Tyr Gln Val Asp Lys Asp Ser Leu Ser Glu Val Ile
385 390 395 400
Tyr Gly Asp Met Asp Lys Leu Leu Cys Pro Asp Gln Ser Glu Gln Ile
405 410 415
Tyr Tyr Thr Asn Asn Ile Val Phe Pro Asn Glu Tyr Val Ile Thr Lys
420 425 430
Ile Asp Phe Thr Lys Lys Met Lys Thr Leu Arg Tyr Glu Val Thr Ala
435 440 445
Asn Phe Tyr Asp Ser Ser Thr Gly Glu Ile Asp Leu Asn Lys Lys Lys
450 455 460
Val Glu Ser Ser Glu Ala Glu Tyr Arg Thr Leu Ser Ala Asn Asp Asp
465 470 475 480
Gly Val Tyr Met Pro Leu Gly Val Ile Ser Glu Thr Phe Leu Thr Pro
485 490 495
Ile Asn Gly Phe Gly Leu Gln Ala Asp Glu Asn Ser Arg Leu Ile Thr
500 505 510
Leu Thr Cys Lys Ser Tyr Leu Arg Glu Leu Leu Leu Ala Thr Asp Leu
515 520 525
Ser Asn Lys Glu Thr Lys Leu Ile Val Pro Pro Ser Gly Phe Ile Ser
530 535 540
Asn Ile Val Glu Asn Gly Ser Ile Glu Glu Asp Asn Leu Glu Pro Trp
545 550 555 560
Lys Ala Asn Asn Lys Asn Ala Tyr Val Asp His Thr Gly Gly Val Asn
565 570 575
Gly Thr Lys Ala Leu Tyr Val His Lys Asp Gly Gly Ile Ser Gln Phe
580 585 590
Ile Gly Asp Lys Leu Lys Pro Lys Thr Glu Tyr Val Ile Gln Tyr Thr
595 600 605
Val Lys Gly Lys Pro Ser Ile His Leu Lys Asp Glu Asn Thr Gly Tyr
610 615 620
Ile His Tyr Glu Asp Thr Asn Asn Asn Leu Glu Asp Tyr Gln Thr Ile
625 630 635 640
Asn Lys Arg Phe Thr Thr Gly Thr Asp Leu Lys Gly Val Tyr Leu Ile
645 650 655
Leu Lys Ser Gln Asn Gly Asp Glu Ala Trp Gly Asp Asn Phe Ile Ile
660 665 670
Leu Glu Ile Ser Pro Ser Glu Lys Leu Leu Ser Pro Glu Leu Ile Asn
675 680 685
Thr Asn Asn Trp Thr Ser Thr Gly Ser Thr Asn Ile Ser Gly Asn Thr
690 695 700
Leu Thr Leu Tyr Gln Gly Gly Arg Gly Ile Leu Lys Gln Asn Leu Gln
705 710 715 720
Leu Asp Ser Phe Ser Thr Tyr Arg Val Tyr Phe Ser Val Ser Gly Asp
725 730 735
Ala Asn Val Arg Ile Arg Asn Ser Arg Glu Val Leu Phe Glu Lys Arg
740 745 750
Tyr Met Ser Gly Ala Lys Asp Val Ser Glu Met Phe Thr Thr Lys Phe
755 760 765
Glu Lys Asp Asn Phe Tyr Ile Glu Leu Ser Gln Gly Asn Asn Leu Tyr
770 775 780
Gly Gly Pro Ile Val His Phe Tyr Asp Val Ser Ile Lys
785 790 795
<210> 117
<211> 793
<212> PRT
<213> 人工序列
<220>
<223> 23718 Vip3
<400> 117
Met Asn Lys Asn Asn Thr Lys Leu Ser Thr Arg Ala Leu Pro Ser Phe
1 5 10 15
Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys Asp
20 25 30
Ile Met Asn Met Ile Phe Lys Thr Asp Thr Gly Gly Asp Leu Thr Leu
35 40 45
Asp Glu Ile Leu Lys Asn Gln Gln Leu Leu Asn Asp Ile Ser Gly Lys
50 55 60
Leu Asp Gly Val Asn Gly Ser Leu Asn Asp Leu Ile Ala Gln Gly Asn
65 70 75 80
Leu Asn Thr Glu Leu Ser Lys Glu Ile Leu Lys Ile Ala Asn Glu Gln
85 90 95
Asn Gln Val Leu Asn Asp Val Asn Asn Lys Leu Asp Ala Ile Asn Thr
100 105 110
Met Leu Arg Val Tyr Leu Pro Lys Ile Thr Ser Met Leu Ser Asp Val
115 120 125
Ile Lys Gln Asn Tyr Ala Leu Ser Leu Gln Ile Glu Tyr Leu Ser Lys
130 135 140
Gln Leu Gln Glu Ile Ser Asp Lys Leu Asp Ile Ile Asn Val Asn Val
145 150 155 160
Leu Ile Asn Ser Thr Leu Thr Glu Ile Thr Pro Ala Tyr Gln Arg Ile
165 170 175
Lys Tyr Val Asn Glu Lys Phe Glu Glu Leu Thr Phe Ala Thr Glu Thr
180 185 190
Ser Ser Ala Glu Ile Leu Glu Glu Thr Glu Asp Gly Ser Pro Ala Asp
195 200 205
Ile Leu Asp Glu Leu Thr Glu Leu Thr Glu Leu Ala Lys Ser Val Thr
210 215 220
Lys Asn Asp Val Asp Gly Phe Glu Phe Tyr Leu Asn Thr Phe His Asp
225 230 235 240
Val Met Val Gly Asn Asn Leu Phe Gly Arg Ser Ala Leu Lys Thr Ala
245 250 255
Ser Glu Leu Ile Thr Lys Glu Asn Val Lys Thr Ser Gly Ser Glu Val
260 265 270
Gly Asn Val Tyr Asn Phe Leu Ile Val Leu Thr Ala Leu Gln Ala Gln
275 280 285
Ala Phe Leu Thr Leu Thr Thr Cys Arg Lys Leu Leu Gly Leu Ala Asp
290 295 300
Ile Asp Tyr Thr Ser Ile Met Asn Glu His Leu Asn Lys Glu Lys Glu
305 310 315 320
Glu Phe Arg Val Asn Ile Leu Pro Thr Leu Ser Asn Thr Phe Ser Asn
325 330 335
Pro Asn Tyr Ala Lys Val Lys Gly Ser Asp Glu Asp Ala Lys Met Ile
340 345 350
Val Glu Ala Lys Pro Gly His Ala Leu Ile Gly Phe Glu Ile Ser Asn
355 360 365
Asp Ser Ile Thr Val Leu Lys Val Tyr Glu Ala Lys Leu Lys Gln Asn
370 375 380
Tyr Gln Val Asp Lys Asp Ser Leu Ser Glu Val Ile Tyr Gly Asp Met
385 390 395 400
Asp Lys Leu Leu Cys Pro Asp Gln Ser Glu Gln Ile Tyr Tyr Thr Asn
405 410 415
Asn Ile Val Phe Pro Asn Glu Tyr Val Ile Thr Lys Ile Asp Phe Thr
420 425 430
Lys Lys Met Lys Thr Leu Arg Tyr Glu Val Thr Ala Asn Phe Tyr Asp
435 440 445
Ser Ser Thr Gly Glu Ile Asp Leu Asn Lys Lys Lys Val Glu Ser Ser
450 455 460
Glu Ala Glu Tyr Arg Thr Leu Ser Ala Asn Asp Asp Gly Val Tyr Met
465 470 475 480
Pro Leu Gly Val Ile Ser Glu Thr Phe Leu Thr Pro Ile Asn Gly Phe
485 490 495
Gly Leu Gln Ala Asp Glu Asn Ser Arg Leu Ile Thr Leu Thr Cys Lys
500 505 510
Ser Tyr Leu Arg Glu Leu Leu Leu Ala Thr Asp Leu Ser Asn Lys Glu
515 520 525
Thr Lys Leu Ile Val Pro Pro Ser Gly Phe Ile Ser Asn Ile Val Glu
530 535 540
Asn Gly Ser Ile Glu Glu Asp Asn Leu Glu Pro Trp Lys Ala Asn Asn
545 550 555 560
Lys Asn Ala Tyr Val Asp His Thr Gly Gly Val Asn Gly Thr Lys Ala
565 570 575
Leu Tyr Val His Lys Asp Gly Gly Ile Ser Gln Phe Ile Gly Asp Lys
580 585 590
Leu Lys Pro Lys Thr Glu Tyr Val Ile Gln Tyr Thr Val Lys Gly Lys
595 600 605
Pro Ser Ile His Leu Lys Asp Glu Asn Thr Gly Tyr Ile His Tyr Glu
610 615 620
Asp Thr Asn Asn Asn Leu Glu Asp Tyr Gln Thr Ile Asn Lys Arg Phe
625 630 635 640
Thr Thr Gly Thr Asp Leu Lys Gly Val Tyr Leu Ile Leu Lys Ser Gln
645 650 655
Asn Gly Asp Glu Ala Trp Gly Asp Asn Phe Ile Ile Leu Glu Ile Ser
660 665 670
Pro Ser Glu Lys Leu Leu Ser Pro Glu Leu Ile Asn Thr Asn Asn Trp
675 680 685
Thr Ser Thr Gly Ser Thr Asn Ile Ser Gly Asn Thr Leu Thr Leu Tyr
690 695 700
Gln Gly Gly Arg Gly Ile Leu Lys Gln Asn Leu Gln Leu Asp Ser Phe
705 710 715 720
Ser Thr Tyr Arg Val Tyr Phe Ser Val Ser Gly Asp Ala Asn Val Arg
725 730 735
Ile Arg Asn Ser Arg Glu Val Leu Phe Glu Lys Arg Tyr Met Ser Gly
740 745 750
Ala Lys Asp Val Ser Glu Met Phe Thr Thr Lys Phe Glu Lys Asp Asn
755 760 765
Phe Tyr Ile Glu Leu Ser Gln Gly Asn Asn Leu Tyr Gly Gly Pro Ile
770 775 780
Val His Phe Tyr Asp Val Ser Ile Lys
785 790
<210> 118
<211> 2370
<212> DNA
<213> 人工序列
<220>
<223> 23705 vip3编码序列
<400> 118
atgaacaaga acaacaccaa gctgagcacc cgcgccctgc cgagcttcat cgactacttc 60
aacggcatct acggcttcgc caccggcatc aaggacatca tgaacatgat cttcaagacc 120
gacaccggcg gcgacctgac cctggacgag atcctgaaga accagcagct gctgaacgac 180
atcagcggca agctggacgg cgtgaacggc agcctgaacg acctgatcgc ccagggcaac 240
ctgaacaccg agctgagcaa ggagatcctt aagatcgcca acgagcagaa ccaggtgctg 300
aacgacgtga acaacaagct ggacgccatc aacaccatgc tgcgcgtgta cctgccgaag 360
atcaccagca tgctgagcga cgtgattaag cagaactacg ccctgagcct gcagatcgag 420
tacctgagca agcagctgca ggagatcagc gacaagctgg acatcatcaa cgtgaacgtc 480
ctgatcaaca gcaccctgac cgagatcacc ccggcctacc agcgcatcaa gtacgtgaac 540
gagaagttcg aagagctgac cttcgccacc gagaccagca gcaaggtgaa gaaggacggc 600
agcccggccg acatcctgga cgagctgacc gagctgaccg agctggcgaa gagcgtgacc 660
aagaacgacg tggacggctt cgagttctac ctgaacacct tccacgacgt gatggtgggc 720
aacaacctgt tcggccgcag cgccctgaag accgccagcg agctgatcac caaggagaac 780
gtgaagacca gcggcagcga ggtgggcaac gtgtacaact tcctgatcgt gctgaccgcc 840
ctgcaggccc aggccttcct gaccctgacc acctgtcgca agctgctggg cctggccgac 900
atcgactaca ccagcatcat gaacgagcac ttgaacaagg agaaggagga gttccgcgtg 960
aacatcctgc cgaccctgag caacaccttc agcaacccga actacgccaa ggtgaagggc 1020
agcgacgagg acgccaagat gatcgtggag gctaagccgg gccacgcgtt gatcggcttc 1080
gagatcagca acgacagcat caccgtgctg aaggtgtacg aggccaagct gaagcagaac 1140
taccaggtgg acaaggacag cttgagcgag gtgatctacg gcgacatgga caagctgctg 1200
tgtccggacc agagcgagca aatctactac accaacaaca tcgtgttccc gaacgagtac 1260
gtgatcacca agatcgactt caccaagaag atgaagaccc tgcgctacga ggtgaccgcc 1320
aacttctacg acagcagcac cggcgagatc gacctgaaca agaagaaggt ggagagcagc 1380
gaggccgagt accgcaccct gagcgcgaac gacgacggcg tctacatgcc actgggcgtg 1440
atcagcgaga ccttcctgac cccgatcaac ggctttggcc tgcaggccga cgagaacagc 1500
cgcctgatca ccctgacctg taagagctac ctgcgcgagc tgctgctagc caccgacctg 1560
agcaacaagg agaccaagct gatcgtgcca ccgagcggct tcatcagcaa catcgtggag 1620
aacggcagca tcgaggagga caacctggag ccgtggaagg ccaacaacaa gaacgcctac 1680
gtcgaccaca ccggcggcgt gaacggcacc aaggccctgt acgtgcacaa ggacggcggc 1740
atcagccagt tcatcggcga caagctgaag ccgaagaccg agtacgtgat ccagtacacc 1800
gtgaagggca agccatcgat tcacctgaag gacgagaaca ccggctacat ccactacgag 1860
gacaccaaca acaacctgga ggactaccag accatcaaca agcgcttcac caccggcacc 1920
gacctgaagg gcgtgtacct gatcctgaag agccagaacg gcgacgaggc ctggggcgac 1980
aacttcatca tcctggagat cagcccgagc gagaagctgc tgagcccgga gctgatcaac 2040
accaacaact ggaccagcac cggcagcacc aacatcagcg gcaacaccct gaccctgtac 2100
cagggcggcc gcggcatcct gaagcagaac ctgcagctgg acagcttcag cacctaccgc 2160
gtgtacttca gcgtgagcgg cgacgccaac gtgcgcatcc gcaactcccg cgaggtgctg 2220
ttcgagaaga ggtacatgag cggcgccaag gacgtgagcg agatgttcac caccaagttc 2280
gagaaggaca acttctacat cgagctgagc cagggcaaca acctgtacgg cggcccgatc 2340
gtgcacttct acgacgtgag catcaagtag 2370
<210> 119
<211> 2424
<212> DNA
<213> 人工序列
<220>
<223> 23716 vip3编码序列
<400> 119
atgtatacgc acacacatgt tgagatatta gaggaaactg aagaattacg ccacattaac 60
aagaacaaca ccaagctgag cacccgcgcc ctgccgagct tcatcgacta cttcaacggc 120
atctacggct tcgccaccgg catcaaggac atcatgaaca tgatcttcaa gaccgacacc 180
ggcggcgacc tgaccctgga cgagatcctg aagaaccagc agctgctgaa cgacatcagc 240
ggcaagctgg acggcgtgaa cggcagcctg aacgacctga tcgcccaggg caacctgaac 300
accgagctga gcaaggagat ccttaagatc gccaacgagc agaaccaggt gctgaacgac 360
gtgaacaaca agctggacgc catcaacacc atgctgcgcg tgtacctgcc gaagatcacc 420
agcatgctga gcgacgtgat taagcagaac tacgccctga gcctgcagat cgagtacctg 480
agcaagcagc tgcaggagat cagcgacaag ctggacatca tcaacgtgaa cgtcctgatc 540
aacagcaccc tgaccgagat caccccggcc taccagcgca tcaagtacgt gaacgagaag 600
ttcgaagagc tgaccttcgc caccgagacc agcagcaagg tgaagaagga cggcagcccg 660
gccgacatcc tggacgagct gaccgagctg accgagctgg cgaagagcgt gaccaagaac 720
gacgtggacg gcttcgagtt ctacctgaac accttccacg acgtgatggt gggcaacaac 780
ctgttcggcc gcagcgccct gaagaccgcc agcgagctga tcaccaagga gaacgtgaag 840
accagcggca gcgaggtggg caacgtgtac aacttcctga tcgtgctgac cgccctgcag 900
gcccaggcct tcctgaccct gaccacctgt cgcaagctgc tgggcctggc cgacatcgac 960
tacaccagca tcatgaacga gcacttgaac aaggagaagg aggagttccg cgtgaacatc 1020
ctgccgaccc tgagcaacac cttcagcaac ccgaactacg ccaaggtgaa gggcagcgac 1080
gaggacgcca agatgatcgt ggaggctaag ccgggccacg cgttgatcgg cttcgagatc 1140
agcaacgaca gcatcaccgt gctgaaggtg tacgaggcca agctgaagca gaactaccag 1200
gtggacaagg acagcttgag cgaggtgatc tacggcgaca tggacaagct gctgtgtccg 1260
gaccagagcg agcaaatcta ctacaccaac aacatcgtgt tcccgaacga gtacgtgatc 1320
accaagatcg acttcaccaa gaagatgaag accctgcgct acgaggtgac cgccaacttc 1380
tacgacagca gcaccggcga gatcgacctg aacaagaaga aggtggagag cagcgaggcc 1440
gagtaccgca ccctgagcgc gaacgacgac ggcgtctaca tgccactggg cgtgatcagc 1500
gagaccttcc tgaccccgat caacggcttt ggcctgcagg ccgacgagaa cagccgcctg 1560
atcaccctga cctgtaagag ctacctgcgc gagctgctgc tagccaccga cctgagcaac 1620
aaggagacca agctgatcgt gccaccgagc ggcttcatca gcaacatcgt ggagaacggc 1680
agcatcgagg aggacaacct ggagccgtgg aaggccaaca acaagaacgc ctacgtcgac 1740
cacaccggcg gcgtgaacgg caccaaggcc ctgtacgtgc acaaggacgg cggcatcagc 1800
cagttcatcg gcgacaagct gaagccgaag accgagtacg tgatccagta caccgtgaag 1860
ggcaagccat cgattcacct gaaggacgag aacaccggct acatccacta cgaggacacc 1920
aacaacaacc tggaggacta ccagaccatc aacaagcgct tcaccaccgg caccgacctg 1980
aagggcgtgt acctgatcct gaagagccag aacggcgacg aggcctgggg cgacaacttc 2040
atcatcctgg agatcagccc gagcgagaag ctgctgagcc cggagctgat caacaccaac 2100
aactggacca gcaccggcag caccaacatc agcggcaaca ccctgaccct gtaccagggc 2160
ggccgcggca tcctgaagca gaacctgcag ctggacagct tcagcaccta ccgcgtgtac 2220
ttcagcgtga gcggcgacgc caacgtgcgc atccgcaact cccgcgaggt gctgttcgag 2280
aagaggtaca tgagcggcgc caaggacgtg agcgagatgt tcaccaccaa gttcgagaag 2340
gacaacttct acatcgagct gagccagggc aacaacctgt acggcggccc gatcgtgcac 2400
ttctacgacg tgagcatcaa gtag 2424
<210> 120
<211> 2394
<212> DNA
<213> 人工序列
<220>
<223> 23717 vip3编码序列
<400> 120
atggctgaga tattagagga aactgaaaac aagaacaaca ccaagctgag cacccgcgcc 60
ctgccgagct tcatcgacta cttcaacggc atctacggct tcgccaccgg catcaaggac 120
atcatgaaca tgatcttcaa gaccgacacc ggcggcgacc tgaccctgga cgagatcctg 180
aagaaccagc agctgctgaa cgacatcagc ggcaagctgg acggcgtgaa cggcagcctg 240
aacgacctga tcgcccaggg caacctgaac accgagctga gcaaggagat ccttaagatc 300
gccaacgagc agaaccaggt gctgaacgac gtgaacaaca agctggacgc catcaacacc 360
atgctgcgcg tgtacctgcc gaagatcacc agcatgctga gcgacgtgat taagcagaac 420
tacgccctga gcctgcagat cgagtacctg agcaagcagc tgcaggagat cagcgacaag 480
ctggacatca tcaacgtgaa cgtcctgatc aacagcaccc tgaccgagat caccccggcc 540
taccagcgca tcaagtacgt gaacgagaag ttcgaagagc tgaccttcgc caccgagacc 600
agcagcaagg tgaagaagga cggcagcccg gccgacatcc tggacgagct gaccgagctg 660
accgagctgg cgaagagcgt gaccaagaac gacgtggacg gcttcgagtt ctacctgaac 720
accttccacg acgtgatggt gggcaacaac ctgttcggcc gcagcgccct gaagaccgcc 780
agcgagctga tcaccaagga gaacgtgaag accagcggca gcgaggtggg caacgtgtac 840
aacttcctga tcgtgctgac cgccctgcag gcccaggcct tcctgaccct gaccacctgt 900
cgcaagctgc tgggcctggc cgacatcgac tacaccagca tcatgaacga gcacttgaac 960
aaggagaagg aggagttccg cgtgaacatc ctgccgaccc tgagcaacac cttcagcaac 1020
ccgaactacg ccaaggtgaa gggcagcgac gaggacgcca agatgatcgt ggaggctaag 1080
ccgggccacg cgttgatcgg cttcgagatc agcaacgaca gcatcaccgt gctgaaggtg 1140
tacgaggcca agctgaagca gaactaccag gtggacaagg acagcttgag cgaggtgatc 1200
tacggcgaca tggacaagct gctgtgtccg gaccagagcg agcaaatcta ctacaccaac 1260
aacatcgtgt tcccgaacga gtacgtgatc accaagatcg acttcaccaa gaagatgaag 1320
accctgcgct acgaggtgac cgccaacttc tacgacagca gcaccggcga gatcgacctg 1380
aacaagaaga aggtggagag cagcgaggcc gagtaccgca ccctgagcgc gaacgacgac 1440
ggcgtctaca tgccactggg cgtgatcagc gagaccttcc tgaccccgat caacggcttt 1500
ggcctgcagg ccgacgagaa cagccgcctg atcaccctga cctgtaagag ctacctgcgc 1560
gagctgctgc tagccaccga cctgagcaac aaggagacca agctgatcgt gccaccgagc 1620
ggcttcatca gcaacatcgt ggagaacggc agcatcgagg aggacaacct ggagccgtgg 1680
aaggccaaca acaagaacgc ctacgtcgac cacaccggcg gcgtgaacgg caccaaggcc 1740
ctgtacgtgc acaaggacgg cggcatcagc cagttcatcg gcgacaagct gaagccgaag 1800
accgagtacg tgatccagta caccgtgaag ggcaagccat cgattcacct gaaggacgag 1860
aacaccggct acatccacta cgaggacacc aacaacaacc tggaggacta ccagaccatc 1920
aacaagcgct tcaccaccgg caccgacctg aagggcgtgt acctgatcct gaagagccag 1980
aacggcgacg aggcctgggg cgacaacttc atcatcctgg agatcagccc gagcgagaag 2040
ctgctgagcc cggagctgat caacaccaac aactggacca gcaccggcag caccaacatc 2100
agcggcaaca ccctgaccct gtaccagggc ggccgcggca tcctgaagca gaacctgcag 2160
ctggacagct tcagcaccta ccgcgtgtac ttcagcgtga gcggcgacgc caacgtgcgc 2220
atccgcaact cccgcgaggt gctgttcgag aagaggtaca tgagcggcgc caaggacgtg 2280
agcgagatgt tcaccaccaa gttcgagaag gacaacttct acatcgagct gagccagggc 2340
aacaacctgt acggcggccc gatcgtgcac ttctacgacg tgagcatcaa gtag 2394
<210> 121
<211> 2382
<212> DNA
<213> 人工序列
<220>
<223> 23718 vip编码序列
<400> 121
atgaacaaga acaacaccaa gctgagcacc cgcgccctgc cgagcttcat cgactacttc 60
aacggcatct acggcttcgc caccggcatc aaggacatca tgaacatgat cttcaagacc 120
gacaccggcg gcgacctgac cctggacgag atcctgaaga accagcagct gctgaacgac 180
atcagcggca agctggacgg cgtgaacggc agcctgaacg acctgatcgc ccagggcaac 240
ctgaacaccg agctgagcaa ggagatcctt aagatcgcca acgagcagaa ccaggtgctg 300
aacgacgtga acaacaagct ggacgccatc aacaccatgc tgcgcgtgta cctgccgaag 360
atcaccagca tgctgagcga cgtgattaag cagaactacg ccctgagcct gcagatcgag 420
tacctgagca agcagctgca ggagatcagc gacaagctgg acatcatcaa cgtgaacgtc 480
ctgatcaaca gcaccctgac cgagatcacc ccggcctacc agcgcatcaa gtacgtgaac 540
gagaagttcg aagagctgac cttcgccacc gagaccagca gcgctgagat attagaggaa 600
actgaagacg gcagcccggc cgacatcctg gacgagctga ccgagctgac cgagctggcg 660
aagagcgtga ccaagaacga cgtggacggc ttcgagttct acctgaacac cttccacgac 720
gtgatggtgg gcaacaacct gttcggccgc agcgccctga agaccgccag cgagctgatc 780
accaaggaga acgtgaagac cagcggcagc gaggtgggca acgtgtacaa cttcctgatc 840
gtgctgaccg ccctgcaggc ccaggccttc ctgaccctga ccacctgtcg caagctgctg 900
ggcctggccg acatcgacta caccagcatc atgaacgagc acttgaacaa ggagaaggag 960
gagttccgcg tgaacatcct gccgaccctg agcaacacct tcagcaaccc gaactacgcc 1020
aaggtgaagg gcagcgacga ggacgccaag atgatcgtgg aggctaagcc gggccacgcg 1080
ttgatcggct tcgagatcag caacgacagc atcaccgtgc tgaaggtgta cgaggccaag 1140
ctgaagcaga actaccaggt ggacaaggac agcttgagcg aggtgatcta cggcgacatg 1200
gacaagctgc tgtgtccgga ccagagcgag caaatctact acaccaacaa catcgtgttc 1260
ccgaacgagt acgtgatcac caagatcgac ttcaccaaga agatgaagac cctgcgctac 1320
gaggtgaccg ccaacttcta cgacagcagc accggcgaga tcgacctgaa caagaagaag 1380
gtggagagca gcgaggccga gtaccgcacc ctgagcgcga acgacgacgg cgtctacatg 1440
ccactgggcg tgatcagcga gaccttcctg accccgatca acggctttgg cctgcaggcc 1500
gacgagaaca gccgcctgat caccctgacc tgtaagagct acctgcgcga gctgctgcta 1560
gccaccgacc tgagcaacaa ggagaccaag ctgatcgtgc caccgagcgg cttcatcagc 1620
aacatcgtgg agaacggcag catcgaggag gacaacctgg agccgtggaa ggccaacaac 1680
aagaacgcct acgtcgacca caccggcggc gtgaacggca ccaaggccct gtacgtgcac 1740
aaggacggcg gcatcagcca gttcatcggc gacaagctga agccgaagac cgagtacgtg 1800
atccagtaca ccgtgaaggg caagccatcg attcacctga aggacgagaa caccggctac 1860
atccactacg aggacaccaa caacaacctg gaggactacc agaccatcaa caagcgcttc 1920
accaccggca ccgacctgaa gggcgtgtac ctgatcctga agagccagaa cggcgacgag 1980
gcctggggcg acaacttcat catcctggag atcagcccga gcgagaagct gctgagcccg 2040
gagctgatca acaccaacaa ctggaccagc accggcagca ccaacatcag cggcaacacc 2100
ctgaccctgt accagggcgg ccgcggcatc ctgaagcaga acctgcagct ggacagcttc 2160
agcacctacc gcgtgtactt cagcgtgagc ggcgacgcca acgtgcgcat ccgcaactcc 2220
cgcgaggtgc tgttcgagaa gaggtacatg agcggcgcca aggacgtgag cgagatgttc 2280
accaccaagt tcgagaagga caacttctac atcgagctga gccagggcaa caacctgtac 2340
ggcggcccga tcgtgcactt ctacgacgtg agcatcaagt ag 2382
<210> 122
<211> 51
<212> DNA
<213> 人工序列
<220>
<223> t基因输入序列
<400> 122
tcctattcaa tgataagaag agcaccgttc actccaactg caaacatgtg g 51
<210> 123
<211> 51
<212> DNA
<213> 人工序列
<220>
<223> t基因输出序列
<400> 123
tcctattcaa ggataagaag agcaccgttc actccaactg caaacaggtg g 51
<210> 124
<211> 51
<212> DNA
<213> 人工序列
<220>
<223> 基序输入序列
<220>
<221> 尚未归类的特征
<223> n = a、c、g或t
<220>
<221> 尚未归类的特征
<222> (1)..(17)
<223> n是a、c、g、或t
<220>
<221> 尚未归类的特征
<222> (39)..(51)
<223> n是a、c、g、或t
<400> 124
nnnnnnnnnn nnnnnnncag agctcccttc actccaaann nnnnnnnnnn n 51
<210> 125
<211> 51
<212> DNA
<213> 人工序列
<220>
<223> 基序输出序列
<400> 125
cgctcacgac agcctggcag agctcccttc actccaaaca ttgcgcatct c 51

Claims (70)

1.一种重组DNA构建体，其包含可操作地连接至编码微小RNA(miRNA)元件的DNA序列的杀昆虫蛋白编码序列，所述miRNA元件在玉米植物中是雄性组织特异性的或雄性组织优选的，其中所述miRNA元件相对于所述杀昆虫蛋白编码序列是异源的。
2.如权利要求1所述的重组DNA构建体，其中所述miRNA元件包含在所述杀昆虫蛋白编码序列的5’非翻译区内、或所述杀昆虫蛋白编码序列的3’非翻译区内、或所述杀昆虫蛋白编码序列内。
3.如权利要求1所述的重组DNA构建体，其中所述雄性组织是花粉和/或绒毡层。
4.如权利要求1至3中任一项所述的重组DNA构建体，其中所述miRNA元件包含编码至少一个miRNA起始子序列的序列。
5.如权利要求4所述的重组DNA构建体，其中所述miRNA起始子序列选自由SEQ ID NO:29-34或SEQ ID NO:94-97组成的组。
6.如权利要求4所述的重组DNA构建体，其中编码所述miRNA起始子序列的所述序列选自由SEQ ID NO:41-46或SEQ ID NO:98-101组成的组。
7.如权利要求5所述的重组DNA构建体，其中与转基因玉米植物中的非雄性生殖组织相比，所述miRNA起始子序列在所述转基因玉米植物中的表达降低了所述杀昆虫蛋白在所述转基因玉米植物的雄性生殖组织中的表达。
8.如权利要求5所述的重组DNA构建体，其中与转基因玉米植物中的非雄性生殖组织相比，所述miRNA起始子序列在所述转基因玉米植物中的表达增加了所述杀昆虫蛋白在所述转基因玉米植物的雄性生殖组织中的表达。
9.如权利要求4所述的重组DNA构建体，其中所述miRNA元件选自由SEQ ID NO:10-18或SEQ ID NO:74-78组成的组。
10.如权利要求1所述的重组DNA构建体，其中所述杀昆虫蛋白编码序列在转基因玉米植物中的表达赋予所述玉米植物对昆虫有害生物的耐受性。
11.如权利要求10所述的重组DNA构建体，其中所述杀昆虫蛋白编码序列编码Cry1蛋白或Vip3蛋白。
12.如权利要求11所述的重组DNA构建体，其中所述Cry1蛋白是Cry1A蛋白，或所述Vip3蛋白是Vip3A蛋白。
13.如权利要求12所述的重组DNA构建体，其中所述Cry1A蛋白是Cry1Ab蛋白，或所述Vip3A蛋白是Vip3Aa蛋白。
14.如权利要求13所述的重组DNA构建体，其中所述Cry1Ab蛋白包含选自由SEQ ID NO:61-66组成的组的氨基酸序列。
15.如权利要求13所述的重组DNA构建体，其中所述Vip3Aa蛋白包含选自由SEQ ID NO:115-117组成的组的氨基酸序列。
16.如权利要求1所述的重组DNA构建体，其中所述构建体包含选自由SEQ ID NO:20-28或SEQ ID NO:85-93组成的组的多核苷酸。
17.一种重组载体，其包含如权利要求1所述的DNA构建体。
18.如权利要求17所述的重组载体，其中所述载体包含选自由SEQ ID NO:49-59或SEQID NO:105-113组成的组的核苷酸序列。
19.一种转基因玉米植物，其包含如权利要求1所述的DNA构建体。
20.一种如权利要求19所述的转基因玉米植物的种子、子代、或植物部分，其中所述种子、子代、或植物部分包含所述DNA构建体。
21.一种制备DNA构建体的方法，所述方法包括鉴定内源性雄性组织特异性或雄性组织优选的玉米微小RNA(miRNA)，构建编码至少一个被所述玉米miRNA识别的miRNA起始子序列的miRNA元件，以及将所述miRNA元件可操作地连接至杀昆虫蛋白编码序列。
22.如权利要求21所述的方法，其中所述雄性组织特异性miRNA或雄性组织优选的miRNA是花粉特异性或花粉优选的或者是绒毡层特异性或绒毡层优选的。
23.如权利要求21所述的方法，其中所述雄性组织特异性miRNA元件或雄性组织优选的miRNA元件是花粉特异性或花粉优选的或者是绒毡层特异性或绒毡层优选的。
24.如权利要求21所述的方法，其中所述miRNA元件包含选自由SEQ ID NO:10-18或SEQID NO:79-83组成的组的多核苷酸。
25.如权利要求21所述的方法，其中由所述miRNA元件编码的所述miRNA起始子序列选自由SEQ ID NO:29-34或SEQ ID NO:94-97组成的组。
26.一种选择性地降低重组杀昆虫蛋白在转基因玉米植物的雄性生殖组织中的表达的方法，所述方法包括在所述转基因玉米植物中表达嵌合基因，所述嵌合基因包含编码所述重组杀昆虫蛋白的杀昆虫蛋白编码序列和可操作地连接至所述杀昆虫蛋白编码序列的至少第一雄性组织特异性miRNA元件或雄性组织优选的miRNA元件，其中所述miRNA元件编码至少一种miRNA起始子序列。
27.如权利要求26所述的方法，其中所述miRNA元件包含在所述杀昆虫蛋白编码序列的5’非翻译区内、或所述杀昆虫蛋白编码序列的3’非翻译区内、或所述杀昆虫蛋白编码序列内。
28.如权利要求26所述的方法，其中所述miRNA元件包含至少两个miRNA起始子序列。
29.如权利要求26所述的方法，其中所述其中所述雄性组织是花粉和/或绒毡层。
30.如权利要求26所述的方法，其中所述miRNA起始子序列选自由SEQ ID NO:29-34或SEQ ID NO:94-97组成的组。
31.如权利要求26所述的方法，其中所述miRNA元件包含选自由SEQ ID NO:10-18或SEQID NO:79-83组成的组的序列。
32.如权利要求26所述的方法，其中所述杀昆虫蛋白编码序列在转基因玉米植物中的表达赋予所述玉米植物对昆虫有害生物的耐受性。
33.如权利要求26所述的方法，其中所述杀昆虫蛋白编码序列编码Cry1蛋白或Vip3蛋白。
34.如权利要求26所述的方法，其中所述杀昆虫蛋白编码序列编码Cry1A蛋白或Vip3A蛋白。
35.如权利要求26所述的方法，其中所述杀昆虫蛋白编码序列编码Cry1Ab蛋白或Vip3Aa蛋白。
36.如权利要求26所述的方法，其中所述杀昆虫蛋白编码序列编码Cry1Ab蛋白，所述Cry1Ab蛋白包含选自由SEQ ID NO:61-66组成的组的氨基酸序列。
37.如权利要求26所述的方法，其中所述杀昆虫蛋白编码序列编码Vip3Aa蛋白，所述Vip3Aa蛋白包含选自由SEQ ID NO:115-117组成的组的氨基酸序列。
38.如权利要求26所述的方法，其中所述DNA构建体包含选自由SEQ ID NO:20-28或SEQID NO:85-93组成的组的序列。
39.一种产生具有增加的雄性能育性的表达Vip3的玉米植物的方法，所述方法包括向玉米植物部分的基因组中引入重组DNA构建体，所述重组DNA构建体包含可操作地连接至编码微小RNA(miRNA)元件的DNA序列的Vip3蛋白编码序列，所述miRNA元件在玉米植物中是雄性组织特异性的或雄性组织优选的，其中所述miRNA元件相对于所述Vip3蛋白编码序列是异源的；由所述玉米植物部分产生玉米植物，从而产生与没有所述DNA构建体的表达Vip3的玉米植物相比具有增加的雄性能育性的表达Vip3的玉米植物。
40.一种产生具有增加的雄性能育性的表达Vip3的玉米植物的方法，所述方法包括：(a)使表达Vip3A的第一玉米植物与第二玉米植物杂交，其中所述第一玉米植物在其基因组中包含重组DNA构建体，所述重组DNA构建体包含可操作地连接至编码微小RNA(miRNA)元件的DNA序列的Vip3蛋白编码序列，所述miRNA元件在玉米植物中是雄性组织特异性的或雄性组织优选的，其中所述miRNA元件相对于所述Vip3蛋白编码序列是异源的；以及任选地(b)使步骤(a)中所得的包含所述DNA构建体的表达Vip3的子代玉米植物与亲本植物回交，以产生回交子代植物；(c)选择包含所述DNA构建体的表达Vip3的子代植物进行回交；以及(d)进行步骤b)和c)至少三次以将所述DNA构建体固定在所希望的遗传背景中，从而产生包含所述DNA构建体并且与不含所述DNA构建体的表达Vip3的玉蜀黍植物相比具有增加的雄性能育性的表达Vip3的玉米植物。
41.一种用于产生具有增加的雄性能育性的表达Vip3的玉米植物或玉米种质的方法，所述方法包括：(a)提供表达Vip3的第一玉米植物或玉米种质，所述第一玉米植物或玉米种质包含重组DNA构建体，所述重组DNA构建体包含可操作地连接至编码微小RNA(miRNA)元件的DNA序列的Vip3蛋白编码序列，所述miRNA元件在玉米植物中是雄性组织特异性的或雄性组织优选的，其中所述miRNA元件相对于所述Vip3蛋白编码序列是异源的；(b)使步骤(a)的所述DNA构建体渗入第二玉米植物或玉米种质中；以及(c)选择包含所述DNA构建体的表达Vip3的玉米植物或玉米种质，从而产生与不含所述DNA构建体的表达Vip3A的玉米植物或玉米种质相比具有增加的雄性能育性的表达Vip3的玉米植物或玉米种质。
42.一种提高表达Vip3的玉蜀黍植物的种子产量的方法，所述方法包括：(a)使第一玉米植物或玉米种质与第二玉米植物或玉米种质杂交，其中所述第一或第二玉米植物或玉米种质表达Vip3杀昆虫蛋白，并且其中所述第一玉米植物或玉米种质在其基因组中包含重组DNA构建体，所述重组DNA构建体包含可操作地连接至编码微小RNA(miRNA)元件的DNA序列的Vip3蛋白编码序列，所述miRNA元件在玉米植物中是雄性组织特异性的或雄性组织优选的，其中所述miRNA元件相对于所述Vip3蛋白编码序列是异源的；以及(b)使用包含所述DNA构建体的子代玉蜀黍植物作为传粉者与其自身或与作为种子亲本起作用的第二玉米植物或玉米种质杂交，从而与合适的对照杂交相比提高所述杂交的种子产量。
43.如权利要求39-42中任一项所述的方法，其中所述miRNA元件包含在所述杀昆虫蛋白编码序列的5’非翻译区内、或所述杀昆虫蛋白编码序列的3’非翻译区内、或所述杀昆虫蛋白编码序列内。
44.如权利要求39-42中任一项所述的方法，其中所述雄性组织是花粉和/或绒毡层。
45.如权利要求39-42中任一项所述的方法，其中所述miRNA元件包含编码至少一个miRNA起始子序列的核苷酸序列。
46.如权利要求45所述的方法，其中由所述核苷酸序列编码的所述miRNA起始子序列选自由SEQ ID NO:94-97组成的组。
47.如权利要求46所述的方法，其中与所述转基因玉米植物中的非雄性生殖组织相比，所述miRNA起始子序列在转基因玉米植物中的表达降低了所述Vip3蛋白在所述转基因玉米植物的雄性生殖组织中的表达。
48.如权利要求45所述的方法，其中所述miRNA元件包含选自由SEQ ID NO:74-78组成的组的序列。
49.如权利要求39-42中任一项所述的方法，其中所述Vip3蛋白编码序列在转基因玉米植物中的表达赋予所述玉米植物对昆虫有害生物的耐受性。
50.如权利要求49所述的方法，其中所述Vip3蛋白是Vip3A蛋白。
51.如权利要求50所述的方法，其中所述Vip3A蛋白是Vip3Aa蛋白。
52.如权利要求51所述的方法，其中所述Vip3Aa蛋白包含选自由SEQ ID NO:115-117组成的组的氨基酸序列。
53.如权利要求39-42中任一项所述的方法，其中所述DNA构建体包含选自由SEQ IDNO:85-93组成的组的多核苷酸。[Vip3表达盒]
54.一种重组杀昆虫蛋白，其对鳞翅目昆虫有活性，其中所述杀昆虫蛋白包含由雄性组织特异性或雄性组织优选的miRNA元件编码的氨基酸序列，并且其中所述miRNA元件编码至少一个miRNA起始子序列。
55.如权利要求54所述的重组杀昆虫蛋白，其中所述杀昆虫蛋白是Cry1蛋白或Vip3蛋白。
56.如权利要求55所述的重组杀昆虫蛋白，其中所述Cry1蛋白是Cry1A蛋白，或所述Vip3蛋白是Vip3A蛋白。
57.如权利要求56所述的重组杀昆虫蛋白，其中所述Cry1A蛋白是Cry1Ab蛋白，并且所述Vip3A蛋白是Vip3Aa蛋白。
58.如权利要求54所述的重组杀昆虫蛋白，其中所述氨基酸序列选自由SEQ ID NO:61-66或SEQ ID NO:115-117组成的组。
59.如权利要求54所述的重组杀昆虫蛋白，其中所述鳞翅目有害生物是欧洲玉米螟或玉米穗蛾。
60.如权利要求54所述的重组杀昆虫蛋白，其中所述雄性组织是花粉或绒毡层。
61.如权利要求54所述的重组杀昆虫蛋白，其中所述miRNA元件包含选自由SEQ ID NO:10-18或SEQ ID NO:74-78组成的组的序列。
62.如权利要求54所述的重组杀昆虫蛋白，其中所述miRNA起始子序列选自由SEQ IDNO:29-34或SEQ ID NO:94-97组成的组。
63.一种合成多核苷酸，其编码如权利要求54-62中任一项所述的重组杀昆虫蛋白。
64.如权利要求63所述的合成多核苷酸，其包含选自由SEQ ID NO:68-73或SEQ ID NO:118-121组成的组的序列。
65.一种杀昆虫组合物，其包含如权利要求54所述的重组杀昆虫蛋白、以及农业上可接受的运载体。
66.一种控制昆虫有害生物的方法，所述方法包括通过转基因玉米植物将有效量的如权利要求54所述的重组杀昆虫蛋白递送至所述昆虫有害生物。
67.如权利要求66所述的方法，其中所述转基因植物包含不同于所述重组杀昆虫蛋白的第二杀昆虫剂。
68.如权利要求67所述的方法，其中所述第二杀昆虫剂是蛋白质或双链RNA(dsRNA)。
69.如权利要求68所述的方法，其中所述蛋白质选自由以下组成的组：Cry蛋白、Vip蛋白、马铃薯糖蛋白、蛋白酶、蛋白酶抑制剂、脲酶、α-淀粉酶抑制剂、成孔蛋白、凝集素、工程化抗体或抗体片段、几丁质酶、及其组合。
70.一种减轻表达杀昆虫蛋白的转基因玉米植物对暴露于来自所述转基因玉米植物的花粉的非靶标昆虫的影响的方法，所述方法包括将如权利要求1所述的DNA构建体引入玉米植物中，其中所述DNA构建体使得所述杀昆虫蛋白在花粉中的表达水平比在叶中更低，从而减轻了所述转基因玉米花粉对暴露于所述花粉的非靶标昆虫的影响。
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