CN113527003B - Green plant disease and pest prevention and control cleaning agent and preparation method and application thereof - Google Patents
Green plant disease and pest prevention and control cleaning agent and preparation method and application thereof Download PDFInfo
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- CN113527003B CN113527003B CN202110782110.8A CN202110782110A CN113527003B CN 113527003 B CN113527003 B CN 113527003B CN 202110782110 A CN202110782110 A CN 202110782110A CN 113527003 B CN113527003 B CN 113527003B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The application discloses a green plant disease and pest prevention and control cleaning agent, and a preparation method and application thereof. The green plant disease and pest prevention and control cleaning agent provided by the application is produced by scientific proportioning of a purely natural biological base, is pollution-free, degradable and pesticide-free, and can block the respiration of plant surface pests (larvae) and eggs by a physical method so as to achieve the purposes of killing and expelling the pests (larvae) and eggs; is rich in mineral substances, fat-soluble and water-soluble vitamins, phospholipids, iron and other trace elements, can promote plant growth, and provides multiple nutrient elements for growth of fruits, vegetables and fruits. The cleaning agent can be applied to plants such as wheat, rice, fruits, vegetables, flowers, tea, chinese medicinal materials and the like, and achieves the effects of no plant diseases and insect pests and no pesticide residues on plant leaves and fruits. Years of researches and field experiments prove that the cleaning agent has stability and reliability, and the cleaning agent is pollution-free, nontoxic, pesticide-free and environment-friendly.
Description
Technical Field
The application relates to a plant cleaning agent, in particular to a green plant disease and pest prevention and control cleaning agent and a preparation method and application thereof.
Background
In the process of agricultural economic development, the production and sales of agricultural products become the main means for acquiring income, and the sales amount of the agricultural products directly affects the income condition of farmers. In order to increase the income of farmers and ensure the quality of agricultural products and provide safe edible products, the management of the pest and disease damage problem must be enhanced.
At present, the crops, especially fruits and vegetables, are insect-proof and insect-repellent, and inorganic pesticides are mainly used. The inorganic pesticide has the characteristics of quick insect killing, poor insect prevention and expelling effect, long pesticide residue time and easy occurrence of various diseases of crops.
Therefore, the green prevention and control technology of the plant diseases and insect pests is actively implemented, the plant diseases and insect pests are not treated by chemical pesticides, and the plant diseases and insect pests of crops are controlled in a biological prevention and control mode. The green prevention and control technology for plant diseases and insect pests can not only effectively boost the quality of agricultural products, ensure that the agricultural products are safer to eat, but also meet the requirements of environmental protection work, and can obtain better social, economic and environmental benefits.
Disclosure of Invention
The application aims to provide a green plant disease and pest prevention and control cleaning agent, and a preparation method and application thereof. The application improves the quality of agricultural products by the green and environment-friendly mode, and ensures people to eat the agricultural products safely.
In order to achieve the above purpose, the present application adopts the following technical scheme:
the application firstly provides a green plant disease and pest prevention and control cleaning agent, which comprises the following components in parts by weight: 0.01 to 0.1 part of mineral substance, 0.01 to 0.1 part of fat-soluble vitamin, 0.01 to 0.1 part of water-soluble vitamin, 100 to 200 parts of fatty acid, 0.01 to 0.1 part of amino acid, 100 to 200 parts of phospholipid, 0.001 to 0.01 part of microelement, 10 to 50 parts of sodium dehydroacetate and 20000 to 30000 parts of water.
In the green plant disease and insect pest prevention and control cleaning agent, the fatty acid contains the following substances in parts by weight: 0.1 to 0.2 part of tetradecanoic acid, 12 to 14 parts of palmitic acid, 0.4 to 0.6 part of palmitoleic acid, 4 to 5 parts of stearic acid, 20 to 22 parts of oleic acid, 50 to 52 parts of linoleic acid, 9 to 10 parts of linolenic acid, 0.3 to 0.5 part of arachic acid, 0.2 to 0.3 part of eicosenoic acid and 0.3 to 0.4 part of behenic acid.
In the green plant disease and insect pest prevention and control cleaning agent, the water-soluble vitamin consists of one or more of the following components in parts by weight: 50-70 parts of D-sodium erythorbate, 1 1-5 parts of vitamin B, 10-20 parts of vitamin B and 6 1-2 parts of vitamin B.
In the green plant disease and insect pest prevention and control cleaning agent, the fat-soluble vitamin consists of one or more of the following components in parts by weight: 0.01 to 0.1 part of vitamin A, 0.01 to 0.1 part of vitamin D and 50 to 70 parts of vitamin K.
In the green plant disease and insect pest prevention and control cleaning agent, the mineral substance consists of one or more of the following components in parts by weight: 400-800 parts of nitrogen, 100-500 parts of phosphorus, 500-1000 parts of potassium and 400-800 parts of calcium.
In the green plant disease and insect pest prevention and control cleaning agent, the trace elements consist of one or more of the following components in parts by weight: 1 to 20 parts of iron, 100 to 200 parts of magnesium, 5 to 10 parts of zinc, 0.5 to 1.5 parts of copper, 0.01 to 0.1 part of selenium, 1 to 5 parts of manganese, 0.01 to 0.05 part of molybdenum, 0.1 to 0.5 part of iodine and 0.01 to 0.1 part of chromium.
In the green plant disease and insect pest prevention and control cleaning agent, the amino acid consists of one or more of the following components in parts by weight: 10-20 parts of lysine, 10-20 parts of tryptophan, 10-20 parts of phenylalanine, 10-20 parts of methionine, 10-20 parts of threonine, 10-20 parts of isoleucine, 10-20 parts of leucine and 10-20 parts of valine.
The application also discloses a preparation method of the green plant disease and pest prevention and control cleaning agent, which comprises the following steps:
s1: mixing the water-soluble vitamins and amino acids in parts by weight with 10-20 parts of water under stirring to obtain a mixture A;
s2: mixing the fatty acid, the phospholipid and the fat-soluble vitamin with the mixture A in parts by weight, and uniformly stirring to obtain a mixture B;
s3: adding the mineral substances, the trace elements and the sodium dehydroacetate into the mixture B in parts by weight, and uniformly stirring to obtain a mixture C;
s4: and mixing the mixture C with 19980-29990 parts of water, and uniformly stirring to obtain the green plant disease and pest control cleaning agent.
The application also discloses application of the cleaning agent for green prevention and control of plant diseases and insect pests in the planting process of wheat, rice, fruits and vegetables, flowers, tea and Chinese medicinal materials as an insect repellent or an insecticide.
The application method comprises the following steps:
s1: uniformly spraying mist in a large area;
s2: re-spraying for 3-5 days after the first spraying;
s3: after finding the insects, locally spraying again;
s4: and re-spraying after 1 month.
Compared with the prior art, the application has the beneficial effects that:
the green plant disease and pest prevention and control cleaning agent provided by the application is produced by scientific proportioning of a purely natural biological base, is pollution-free, degradable and pesticide-free, and can block the respiration of plant surface pests (larvae) and eggs by a physical method so as to achieve the purposes of killing and expelling the pests (larvae) and eggs; is rich in mineral substances, fat-soluble and water-soluble vitamins, phospholipids, iron and other trace elements, can promote plant growth, and provides multiple nutrient elements for plant growth. The green plant disease and pest prevention and control cleaning agent can be applied to plants such as wheat, rice, fruits and vegetables, flowers, tea, chinese medicinal materials and the like, and achieves the effects of no disease and pest damage and no pesticide residue on plant leaves and fruits. Years of researches and field experiments prove that the green plant disease and pest prevention and control cleaning agent has stability and reliability, and can be pollution-free, nontoxic, pesticide residue-free and environment-friendly.
Detailed Description
In order to enable those skilled in the art to better understand the technical solutions of the present application, the present application is further described below with reference to examples:
the application relates to a green plant disease and pest prevention and control cleaning agent, which comprises the following components in parts by weight: 0.01 to 0.1 part of mineral substance, 0.01 to 0.1 part of fat-soluble vitamin, 0.01 to 0.1 part of water-soluble vitamin, 100 to 200 parts of fatty acid, 0.01 to 0.1 part of amino acid, 100 to 200 parts of phospholipid, 0.001 to 0.01 part of microelement, 10 to 50 parts of sodium dehydroacetate and 20000 to 30000 parts of water. Wherein:
the fat-soluble vitamin consists of one or more of the following components in parts by weight: 0.01 to 0.1 part of vitamin A, 0.01 to 0.1 part of vitamin D and 50 to 70 parts of vitamin K. Preferably, it is: 0.05 part of vitamin A, 0.05 part of vitamin D and 60 parts of vitamin K.
The mineral substance consists of one or more of the following components in parts by weight: 400-800 parts of nitrogen, 100-500 parts of phosphorus, 500-1000 parts of potassium and 400-800 parts of calcium. Preferably, it is: 600 parts of nitrogen, 300 parts of phosphorus, 800 parts of potassium and 500 parts of calcium.
The microelements comprise one or more of the following components in parts by weight: 1 to 20 parts of iron, 100 to 200 parts of magnesium, 5 to 10 parts of zinc, 0.5 to 1.5 parts of copper, 0.01 to 0.1 part of selenium, 1 to 5 parts of manganese, 0.01 to 0.05 part of molybdenum, 0.1 to 0.5 part of iodine and 0.01 to 0.1 part of chromium. Preferably, it is: 10 parts of iron, 100 parts of magnesium, 10 parts of zinc, 1 part of copper, 0.05 part of selenium, 1 part of manganese, 0.05 part of molybdenum, 0.5 part of iodine and 0.01 part of chromium.
The amino acid consists of one or more of the following components in parts by weight: 10-20 parts of lysine, 10-20 parts of tryptophan, 10-20 parts of phenylalanine, 10-20 parts of methionine, 10-20 parts of threonine, 10-20 parts of isoleucine, 10-20 parts of leucine and 10-20 parts of valine. Preferably, it is: 10 parts of lysine, 10 parts of tryptophan, 10 parts of phenylalanine, 10 parts of methionine, 10 parts of threonine, 10 parts of isoleucine, 10 parts of leucine and 10 parts of valine.
Several representative cleaning agent formulations were selected from the preferred ratios as shown in the following table:
the preparation method of the green plant disease and pest prevention and control cleaning agent comprises the following steps:
s1: mixing the water-soluble vitamins and amino acids in parts by weight with a small amount of water under stirring to obtain a mixture A;
s2: mixing the fatty acid, the phospholipid and the fat-soluble vitamin with the mixture A in parts by weight, and uniformly stirring to obtain a mixture B;
s3: adding the mineral substances, the trace elements and the sodium dehydroacetate into the mixture B in parts by weight, and uniformly stirring to obtain a mixture C;
s4: and mixing the mixture C with the rest water, and uniformly stirring to obtain the green plant disease and pest control cleaning agent.
Test example 1 indoor insecticidal Activity assay
1. Test agent:
test agent: 1-8 parts of cleaning agent.
Control agent: 95% imidacloprid technical, hebei Wilkinson chemical Co., ltd.
2. Test subject:
a sensitive strain of Aphis faveolata (Aphis craccivora) nymph, which is bred for perennial indoor.
3. Test conditions:
temperature: 24-26 DEG C
Humidity: RH60%
Illumination: l: d=14:10
4. The test method comprises the following steps:
preparing test liquid medicine:
taking 1-8 parts of cleaning agent, adding 9 times of water to prepare mother solution with concentration, and diluting with water to obtain serial medicinal solutions with concentration gradients of 0.5, 0.25, 0.125, 0.0625 and 0.03125.
Preparing a control liquid medicine:
taking imidacloprid, completely dissolving in solvent, adding proper amount of emulsifying agent, adding water to prepare mother solution with concentration, and diluting with water according to the dose of test design to obtain a series of medicinal liquids with concentration gradients of 0.2, 0.1, 0.05, 0.025 and 0.0125.
The treatment method comprises the following steps:
soaking the bean seedlings with the bean aphid Aphis faveolata in the prepared liquid medicine for 10s, removing excessive liquid medicine, inserting into a water culture sponge tray, covering with a glass cover, and transferring into an observation chamber for culture. The test was run with a blank solvent control and a clear water treatment control, and each treatment was repeated 4 times.
The investigation method comprises the following steps:
and (5) placing the treated test insects in an observation chamber, periodically observing the reaction condition of the test insects, and investigating the number of dead and alive insects after 72 hours.
The calculation method comprises the following steps:
correcting mortality by abbott's formula, application of computer programSolving a toxicity regression equation and LC of the tested medicament 50 Values and 95% confidence limits according to LC 50 The values evaluate the indoor virulence of the test agents and statistically analyze the significance of differences between samples.
5. Test results
LC (liquid crystal) cleaning agent 1-8 pairs of broad bean insect nymphs 50 The values are 154.4786mg/L, 81.2156mg/L, 187.5328mg/L, 217.8217mg/L, 986.7823mg/L, 231.2389mg/L, 734.2745mg/L and 172.8924mg/L respectively, and the contrast agent is LC to the nymph of broad bean 50 The value is 0.0156mg/L.
From the test results, the tested sample has certain activity on the broad bean, and can be theoretically used for preventing and controlling the insect in the thousand fields, but the activity of the test reagent cleaning agent 1-8 is far lower than that of the chemical pesticide, and the dosage of the test reagent cleaning agent should be properly increased when the test reagent cleaning agent is applied in the fields. Meanwhile, the activity of the cleaning agent 2 is higher than that of the cleaning agent 1, which shows that the cleaning agent with higher concentration has better insecticidal effect; the activity of the cleaning agents 1, 2, 3, 4, 6 and 8 is obviously higher than that of the cleaning agents 5 and 7, which indicates that the fatty acid and the phospholipid play a key role in the insecticidal effect of the cleaning agents; compared with the cleaning agents 5 and 7, the cleaning agents have the advantages that the influence of the fatty acid on the insecticidal effect of the cleaning agents is larger; the differences among the cleaning agents 3, 4, 6 and 8 are not obvious, which indicates that the addition of minerals, vitamins, amino acids and microelements has little influence on the insecticidal effect of the cleaning agents; the activity of the cleaning agent 2 is higher than that of the cleaning agent 1, and the activities of the cleaning agents 1 and 2 are higher than those of other groups, which indicates that the cleaning agent containing various nutrient elements has better insecticidal effect.
Test example 2 pesticide field efficacy test
1. Test agent:
test agent: 1-8 parts of cleaning agent.
Control agent: 10% imidacloprid wettable powder, which is produced by Hebei Wilkinson chemical Co., ltd.
2. Selection of test subjects, crops and varieties:
test object: worm, school name: sitobion avena, sitobion avena Sehizaphis graminum.
Test crop: wheat, variety Jimai 22.
3. Test conditions:
temperature: the temperature is 19-29 ℃ and the average temperature is 24 DEG C
Humidity: RH30%
Southwest wind level 3, cloudy weather
The test land is loam, the soil fertility is uniform, the pH value of the soil is 7.2/slightly alkaline, and the organic matter content is 2.1%. No watering was applied during the test.
4. The test method comprises the following steps:
using knapsack sprayer (JACTO HD 400) produced by Singapore private Co., ltd, uniformly spraying the front and back sides of the blade, and using 750 liters (750L/hm) of the liquid medicine per hectare 2 )。
| Medicament treatment | Dosage of preparation (g/mu) |
| Test agent | 100 |
| Control agent | 10 |
| Blank control | 0 |
Test agent detergent 1-8, control agent and blank control, 10 treatments in total, and the cells of each treatment were randomly arranged.
Suitable administration period and dosage: in the early stage of wheat insect, when the number of the insect is greater than 500, the pesticide is applied once at the dosage of 50 kg/mu, and the pesticide is uniformly sprayed on all parts of the damaged plant.
The basal number was investigated before administration, and the basal number was investigated once each of 1, 3 and 7 days after administration, 4 times in total.
Five samples were taken per cell, 2 aphid plants were fixed per spot, and the number of larvae on the fixed plants was investigated.
The prevention and treatment effect is represented by the corrected rate of the reduction of the insect population, and the statistical calculation is carried out according to a method specified by pesticide field efficacy test criterion (II), wherein the calculation formula is as follows:
reduction rate of insect population (%) = [ (number of live insects before administration-number of live insects after administration)/number of live insects before administration ] ×100
Control effect (%) = [ (rate of reduction of insect population in treated area-rate of reduction of insect population in control area)/(rate of reduction of insect population in control area) ], 100
5. Test results:
the control (%) shown in the above table is the average value of each repetition.
And adopting DPS data processing software to perform single-factor analysis of variance on the test data.
Drug evaluation: from the test results, the cleaning agents 1 and 2 have better control effects on wheat larvae, the control effects on 1 day, 3 days and 7 days after application are 42%, 85%, 44%, 87% and 86%, and the control effects are not obvious from the control agents. The ladybug larvae or adults are found in each investigation period, which indicates that the medicament has very high safety to natural enemies. No adverse effect on wheat, non-target beneficial insects, etc. was found at the amount of agent used.
The above examples are only illustrative of the preferred embodiments of the present application and are not intended to limit the scope of the present application, and various modifications and improvements made by those skilled in the art to the technical solution of the present application should fall within the scope of protection defined by the claims of the present application without departing from the spirit of the present application.
Claims (4)
1. The green plant disease and pest prevention and control cleaning agent is characterized by comprising the following components in parts by weight: 0.01-0.1 part of mineral, 0.01-0.1 part of fat-soluble vitamin, 0.01-0.1 part of water-soluble vitamin, 100-200 parts of fatty acid, 0.01-0.1 part of amino acid, 100-200 parts of phospholipid, 0.001-0.01 part of trace element, 10-50 parts of sodium dehydroacetate and 20000-30000 parts of water; the preparation method comprises the following steps:
s1: mixing the water-soluble vitamins and amino acids in parts by weight with 10-20 parts of water under stirring to obtain a mixture A;
s2: mixing the fatty acid, the phospholipid and the fat-soluble vitamin with the mixture A in parts by weight, and uniformly stirring to obtain a mixture B;
s3: adding the mineral substances, the trace elements and the sodium dehydroacetate into the mixture B in parts by weight, and uniformly stirring to obtain a mixture C;
s4: mixing the mixture C with 19980-29990 parts of water, and uniformly stirring to obtain a green plant disease and pest prevention and control cleaning agent;
the fatty acid contains the following substances in parts by weight: 0.1-0.2 part of tetradecanoic acid, 12-14 parts of palmitic acid, 0.4-0.6 part of palmitoleic acid, 4-5 parts of stearic acid, 20-22 parts of oleic acid, 50-52 parts of linoleic acid, 9-10 parts of linolenic acid, 0.3-0.5 part of arachidic acid, 0.2-0.3 part of eicosenoic acid and 0.3-0.4 part of behenic acid;
the water-soluble vitamin consists of one or more of the following components in parts by weight: 50-70 parts of D-sodium erythorbate, 1-5 parts of vitamin B, 10-20 parts of vitamin B and 1-2 parts of vitamin B;
the fat-soluble vitamin consists of one or more of the following components in parts by weight: 0.01-0.1 part of vitamin A, 0.01-0.1 part of vitamin D and 50-70 parts of vitamin K;
the mineral substance consists of one or more of the following components in parts by weight: 400-800 parts of nitrogen, 100-500 parts of phosphorus, 500-1000 parts of potassium and 400-800 parts of calcium;
the trace elements consist of one or more of the following components in parts by weight: 1-20 parts of iron, 100-200 parts of magnesium, 5-10 parts of zinc, 0.5-1.5 parts of copper, 0.01-0.1 part of selenium, 1-5 parts of manganese, 0.01-0.05 part of molybdenum, 0.1-0.5 part of iodine and 0.01-0.1 part of chromium;
the amino acid consists of one or more of the following components in parts by weight: 10-20 parts of lysine, 10-20 parts of tryptophan, 10-20 parts of phenylalanine, 10-20 parts of methionine, 10-20 parts of threonine, 10-20 parts of isoleucine, 10-20 parts of leucine and 10-20 parts of valine.
2. The method for preparing the green plant disease and pest prevention and control cleaning agent according to claim 1, which is characterized by comprising the following steps:
s1: mixing the water-soluble vitamins and amino acids in parts by weight with 10-20 parts of water under stirring to obtain a mixture A;
s2: mixing the fatty acid, the phospholipid and the fat-soluble vitamin with the mixture A in parts by weight, and uniformly stirring to obtain a mixture B;
s3: adding the mineral substances, the trace elements and the sodium dehydroacetate into the mixture B in parts by weight, and uniformly stirring to obtain a mixture C;
s4: and mixing the mixture C with 19980-29990 parts of water, and uniformly stirring to obtain the green plant disease and pest control cleaning agent.
3. The use of the cleaning agent for green prevention and control of plant diseases and insect pests according to claim 1 as an insect repellent or pesticide in the planting process of wheat, rice, fruits and vegetables, flowers, tea leaves and Chinese medicinal materials.
4. The use according to claim 3, characterized in that the method steps comprise:
s1: uniformly spraying mist in a large area;
s2: re-spraying for 3-5 days after the first spraying;
s3: after finding the insects, locally spraying again;
s4: and re-spraying after 1 month.
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