CN111264545A - Composition for preventing and treating aphids, insecticide and application - Google Patents

Composition for preventing and treating aphids, insecticide and application Download PDF

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Publication number
CN111264545A
CN111264545A CN202010164177.0A CN202010164177A CN111264545A CN 111264545 A CN111264545 A CN 111264545A CN 202010164177 A CN202010164177 A CN 202010164177A CN 111264545 A CN111264545 A CN 111264545A
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Prior art keywords
composition
aphids
matrine
pymetrozine
insecticide
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Inventor
吴静
陆静
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Jiangsu Kesheng Crop Technology Co ltd
JIANGSU KWIN GROUP
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Jiangsu Kesheng Crop Technology Co ltd
JIANGSU KWIN GROUP
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/7071,2,3- or 1,2,4-triazines; Hydrogenated 1,2,3- or 1,2,4-triazines

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides a composition for preventing and treating aphids, an insecticide and application, belonging to the technical field of pest control; the composition comprises the following components: matrine and pymetrozine. In the invention, the matrine has contact poisoning and stomach poisoning effects on aphids; pymetrozine has a needle blocking effect on aphids, so that the aphids stop feeding and die due to starvation; the matrine and the pymetrozine have different action mechanisms, are not easy to generate drug resistance after being compounded, prolong the service life of the pesticide, reduce the use cost and lighten the pollution to the environment. The matrine and pymetrozine in the composition have obvious synergistic action.

Description

Composition for preventing and treating aphids, insecticide and application
Technical Field
The invention relates to the technical field of pest control, and particularly relates to a composition for controlling aphids, an insecticide and application.
Background
Aphids, also known as putties and nectasis, are a group of phytophagous pests. At present, the control of the pesticide is mainly chemical pesticide control, and the commonly used pesticide is mainly imidacloprid, thiamethoxam, buprofezin, chlorpyrifos and the like. With the long-term repeated use of pesticides, the resistance of pests to conventional pesticides is higher and higher, and the control effect is worse and worse. At present, in the aphid control process, no record or report exists for combining a matrine water agent and pymetrozine.
Disclosure of Invention
The invention aims to provide a composition for preventing and treating aphids, an insecticide and application thereof.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a composition for preventing and treating aphids, which comprises the following components: matrine and pymetrozine; the mass ratio of the matrine to the pymetrozine is 1-5: 10 to 80 parts.
Preferably, the mass ratio of the matrine to the pymetrozine is 2-3: 20 to 50.
The invention provides an insecticide comprising the composition, wherein the matrine in the insecticide accounts for 0.05-0.3% by mass; the mass percentage content of pymetrozine in the pesticide is 1-24%.
Preferably, the formulation of the pesticide comprises an aqueous emulsion, a microemulsion or a suspension.
The invention provides application of the composition or the pesticide in the scheme to prevention and treatment of aphids.
Preferably, the aphids comprise one or more of myzus persicae, Aphis citricola and Aphis gossypii.
Preferably, the application comprises the following steps: and (3) diluting the composition or the pesticide by 1000-6000 times, and spraying the composition or the pesticide on insect pest crops.
Preferably, the spraying period is the pest occurrence period of aphids; the spraying frequency is 1-2 times, and the interval time of the two times of spraying is 7-14 days.
Preferably, the application amount of the diluted composition or the diluted pesticide is 10-100L/mu.
The invention has the beneficial effects that: the invention provides a composition for preventing and treating aphids, which comprises the following components: matrine and pymetrozine. In the invention, the matrine has contact poisoning and stomach poisoning effects on aphids; pymetrozine has a needle blocking effect on aphids, so that the aphids stop feeding and die due to starvation; the matrine and the pymetrozine have different action mechanisms, are not easy to generate drug resistance after being compounded, prolong the service life of the pesticide, reduce the use cost and lighten the pollution to the environment. The matrine and pymetrozine in the composition have obvious synergistic action. Compared with a single agent, the composition of the invention has better control effect in controlling aphids, and reduces the use amount of the single agent. The matrine and pymetrozine in the composition have obvious synergistic action.
Detailed Description
The invention provides a composition for preventing and treating aphids, which comprises the following components: matrine and pymetrozine; the mass ratio of the matrine to the pymetrozine is 1-5: 10-80, preferably 2-3: 20-50, more preferably 2.5: 30-40 parts of; the matrine and pymetrozine are from conventional market.
In the invention, the matrine has contact killing effect and stomach toxicity effect on aphids, once the aphids contact the matrine, nerve centers can be paralyzed, and then body proteins of pests are solidified to block pores of the pests so as to suffocate the pests, so that the pests can be killed quickly; pymetrozine has a needle blocking effect on aphids, once aphids contact pymetrozine, feeding can be stopped immediately, and starvation is finally caused; the matrine and the pymetrozine have different action mechanisms, are not easy to generate drug resistance after being compounded, prolong the service life of the pesticide, reduce the use cost and lighten the pollution to the environment.
The invention provides an insecticide comprising the composition, wherein the matrine in the insecticide accounts for 0.05-0.3% by mass, and preferably accounts for 0.1-0.2%; the mass percentage content of the pymetrozine in the pesticide is 1-24%, preferably 5-20%, and more preferably 10%.
In the invention, the dosage form of the pesticide preferably comprises aqueous emulsion, microemulsion and suspending agent; the pesticide preferably also comprises auxiliary materials; the invention has no special limitation on the types and the dosage of the auxiliary materials, and the auxiliary materials can be conventional auxiliary materials in the field.
The invention provides the application of the composition or the pesticide in the scheme in preventing and controlling aphids; in the invention, the aphids preferably comprise one or more of green peach aphids, apple yellow aphids and cotton aphids.
In the invention, the application comprises the following steps: after the composition or the pesticide is diluted by 1000-6000 times, the composition or the pesticide is sprayed on insect-pest crops; the dilution multiple of the composition or the pesticide is preferably 2000-5000 times, and more preferably 3000-4000 times; the reagent used for dilution is preferably water; the spraying period is preferably the pest period of aphids; the spraying frequency is preferably 1-2 times, and the interval time between two times of spraying is preferably 7-14 days; the application amount of the diluted composition or pesticide is preferably 10-100L/mu, and more preferably 30-80L/mu.
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1 indoor virulence assay
1. Pharmaceutical formulation
The selected medicaments are as follows:
single dose: 50 percent of pymetrozine wettable powder (purchased from Hebei Bojia agriculture company Limited) and 0.3 percent of matrine aqueous solution (purchased from Weifang Aofeng crop disease control company Limited).
The compound insecticidal composition comprises the following components: the mass ratio of the matrine to the pymetrozine is 1: 80. 2: 70. 3: 60. 4: 50. 5: 40. 2: 10 and 5: 10, the mixture also comprises conventional auxiliary materials.
The single-dose and compound insecticidal composition is diluted to 5000mg/L mother liquor for later use, and each single-dose and each group of mixture are respectively diluted into 5-7 concentration gradients.
2. Processing method
The toxicity effect of the insecticide on aphids is determined by an insect-soaking method. Collecting fresh peach tree tender tips with aphids from the field, removing redundant aphids, keeping healthy wingless adult aphids with basically consistent sizes, soaking the healthy wingless adult aphids in a pesticide solution for 5s, taking out and airing the adult aphids, placing the adult aphids into a culture dish which is padded with filter paper and has the diameter of 90mm, adding water into the culture dish for moisturizing, treating each mass concentration of the pesticide-soaked solution for 1 time, repeating each treatment for 3 times, and repeating each treatment for 30 aphids.
3. Raising and observing
The processed aphids are placed at the temperature of 25 +/-1 ℃, the relative humidity of 60 percent and the photoperiod of L: and D ═ 16: 8) h for feeding and observation.
4. Examination of results
After 24h, the death condition of each treated pest is checked, and the number of live pests and the number of dead pests are recorded respectively.
5. Data statistics
The insecticidal activity of the matrine aqua with the mass percentage of 0.3 percent and the pymetrozine wettable powder with the mass percentage of 50 percent in different proportions of insecticides is evaluated by a co-toxicity coefficient (CTC) method provided by Sun Yunpei and the like.
Virulence index TI ═ (LC of standard agent)50LC of test agent50)×100
Actual Toxicity Index (ATI) of mixed preparation (LC of standard preparation)50LC of/mixture50)×100
The Theoretical Toxicity Index (TTI) of the mixed preparation (the percentage (%) of the TI × A preparation of the A preparation) plus the percentage (%) of the TI × B preparation of the B preparation in the mixed preparation)
Co-toxicity coefficient (CTC) ═ Actual Toxicity Index (ATI) of the mixture/[ Theoretical Toxicity Index (TTI) of the mixture) ] x l00
According to the regulation of agricultural chemical examination institute of Ministry of agriculture, the judgment standard of the synergy of the mixture formula is as follows: the co-toxicity coefficient is more than 120, so that the synergistic effect is achieved; the co-toxicity coefficient is 80-120, which is an additive effect; the co-toxicity coefficient is less than 80, and the antagonism is obtained.
And (3) carrying out statistical analysis on different proportions, carrying out statistical analysis on indoor live test by adopting an SPSS 20.0 statistical analysis system, solving a toxicity regression equation of the medicament, and calculating LC50 and a correlation coefficient.
6. Results and analysis
TABLE 1 indoor toxicity test results of matrine aqua with different mixture ratios and mass percentage content of 0.3 percent and pymetrozine wettable powder with mass percentage content of 50 percent
Figure BDA0002406809460000041
Figure BDA0002406809460000051
The results of indoor toxicity tests in table 1 show that both the matrine aqueous solution with a mass percentage of 0.3% and the pymetrozine wettable powder with a mass percentage of 50% have certain control effects on myzus persicae, and the mass ratio of the matrine aqueous solution with a mass percentage of 0.3% to the pymetrozine wettable powder with a mass percentage of 50% is 25: 12-250: 3 shows synergy, with 25: 12 showed the most significant synergy.
Example 2: composition of matrine and pymetrozine has field control effect on Aphis citricola
1. Conditions of treatment
The object to be treated is yellow aphid, the crop to be tested is apple tree, and the variety is Fuji.
The treated Aphis citricola occurs universally and uniformly throughout the year; the growth period of the apples during the treatment is the fruit expansion period, and the cultivation conditions (soil type, fertilization, growth stage, plant row spacing and the like) and the growth vigor of plants in all treatment districts are uniform and consistent with local cultivation measures.
2. Flow of treatment
Treating the medicament: 0.3 percent of matrine aqueous solution by mass percent, 50 percent of pymetrozine wettable powder insecticidal composition (25: 12) by mass percent; the reference medicament is a matrine aqua with the mass percentage of 0.3 percent; 50 percent of pymetrozine wettable powder. Treating the mixed solution to obtain 0.3 mass percent of matrine aqueous solution and 50 mass percent of pymetrozine wettable powder insecticidal composition (25: 12)2000 times of solution, 4000 times of solution and 6000 times of solution; 0.3 percent of matrine aqueous 2000 times liquid; 5 treatments of 2000 times of liquid of 50% pymetrozine wettable powder by mass percentage are carried out, each treatment is repeated for 3 trees, 1 repetition is 1 cell, and all cells are arranged randomly.
3. Method of administering a drug
Diluting the pesticide by adding water according to a specified multiple in the emergence period of Aphis citricola, and spraying the whole apple trees by using a sprayer, wherein the standard is that the pesticide is uniformly applied to the front and back of leaves, and the water consumption per mu is 100L.
4. Investigation and recording method
According to the field efficacy treatment criterion GB/T17980.65-2004, the population base number of the insects is investigated before the pesticide is applied, and the number of the residual and alive insects is investigated on 3 rd, 7 th and 14 th days after the pesticide is applied for 4 times. Investigating 2 trees in each cell, fixing 5 damaged tips in east, west, south, north and middle of each tree, and investigating the number of live aphids with 5-10 leaves on the top tip of each tip.
The drug effect calculation method comprises the following steps: control effect (%) < 1- (live insect quantity before drug administration in the clear water control area x live insect quantity after drug administration in the drug treatment area)/(live insect quantity after drug administration in the clear water control area x live insect quantity before drug administration in the drug treatment area) ] × 100
5. Results and analysis
TABLE 2 field treatment results for the test agents
Figure BDA0002406809460000061
Note: the data in the table are the average data of each treatment.
As can be seen from Table 2, the matrine aqueous solution with the mass percentage of 0.3 percent and the pymetrozine wettable powder insecticidal composition (25: 12) with the mass percentage of 50 percent have better control effect on Aphis citricola, and the control effect is increased along with the increase of the dosage. The control effect of each treatment of the composition is superior to that of a single control agent.
Example 3 safety analysis of the composition of matrine and pymetrozine of the present invention
The toxicity test result of a determined institution shows that the compound composition (0.3 percent by mass of matrine aqua, 50 percent by mass of pymetrozine wettable powder insecticidal composition (25: 12)) belongs to a micro-toxic pesticide:
rat acute oral toxicity LD50 of more than 5000mg/kg and male parent of more than 5000 mg/kg;
rat acute percutaneous toxicity LD50 of greater than 5000mg/kg and male parent of greater than 5000 mg/kg;
the integral of the rabbit eye irritation is 0(1h), and the rabbit eye irritation is diluted by 0 (1: 100) after 48h, so that the rabbit eye irritation is not irritant to eyes;
the integral of the rabbit eye irritation is 0(4h), and the rabbit eye irritation is not irritant to skin.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (9)

1. A composition for controlling aphids, said composition comprising the following components: matrine and pymetrozine; the mass ratio of the matrine to the pymetrozine is 1-5: 10 to 80 parts.
2. The composition according to claim 1, wherein the mass ratio of the matrine to the pymetrozine is 2-3: 20 to 50.
3. An insecticide comprising the composition of claim 1 or 2, wherein the matrine accounts for 0.05 to 0.3 percent of the insecticide by mass; the mass percentage content of pymetrozine in the pesticide is 1-24%.
4. The insecticide according to claim 3, wherein said insecticide is in the form of an aqueous emulsion, microemulsion or suspension.
5. Use of a composition according to claim 1 or 2 or of an insecticide according to claim 3 or 4 for controlling aphids.
6. The use according to claim 5, wherein said aphids comprise one or more of the species Myzus persicae, Aphis citricola and Aphis gossypii.
7. The application according to claim 5, characterized in that it comprises the following steps: and (3) diluting the composition or the pesticide by 1000-6000 times, and spraying the composition or the pesticide on insect pest crops.
8. The use according to claim 7, characterized in that the spraying period is the pest emergence period of aphids; the spraying frequency is 1-2 times, and the interval time of the two times of spraying is 7-14 days.
9. The use according to claim 7 or 8, wherein the diluted composition or insecticide is applied in an amount of 10 to 100L/acre.
CN202010164177.0A 2020-03-11 2020-03-11 Composition for preventing and treating aphids, insecticide and application Pending CN111264545A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112262723A (en) * 2020-11-05 2021-01-26 青海省农林科学院 Whole-course biological prevention and control method for diseases and insect pests of broad beans

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CN105145621A (en) * 2015-09-28 2015-12-16 全椒县花溪湖特种水产合作社 Drug preventing and curing tobacco aphids
CN106982640A (en) * 2017-05-24 2017-07-28 安徽农业大学 A kind of sandy loess wheat stalk crushes corn machine live broadcasting method under returning to the field
CN107372490A (en) * 2017-07-27 2017-11-24 兰溪市顺光园艺技术有限公司 The compound brown paddy plant hopper processing method of compound insecticide of rice
CN107549204A (en) * 2017-07-27 2018-01-09 浦江县合洪园艺研发有限公司 The purposes of the compound brown paddy plant hopper composite insecticide of rice

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Publication number Priority date Publication date Assignee Title
CN105145621A (en) * 2015-09-28 2015-12-16 全椒县花溪湖特种水产合作社 Drug preventing and curing tobacco aphids
CN106982640A (en) * 2017-05-24 2017-07-28 安徽农业大学 A kind of sandy loess wheat stalk crushes corn machine live broadcasting method under returning to the field
CN107372490A (en) * 2017-07-27 2017-11-24 兰溪市顺光园艺技术有限公司 The compound brown paddy plant hopper processing method of compound insecticide of rice
CN107549204A (en) * 2017-07-27 2018-01-09 浦江县合洪园艺研发有限公司 The purposes of the compound brown paddy plant hopper composite insecticide of rice

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安然等: "苹果园病虫害的局部发生与挑治", 《北方果树》 *
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112262723A (en) * 2020-11-05 2021-01-26 青海省农林科学院 Whole-course biological prevention and control method for diseases and insect pests of broad beans
CN112262723B (en) * 2020-11-05 2023-06-23 青海省农林科学院 Whole-course biological prevention and control method for broad bean diseases and insect pests

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Application publication date: 20200612