CN113511935A - Plant nutrient absorption enhancer - Google Patents
Plant nutrient absorption enhancer Download PDFInfo
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- CN113511935A CN113511935A CN202110454771.8A CN202110454771A CN113511935A CN 113511935 A CN113511935 A CN 113511935A CN 202110454771 A CN202110454771 A CN 202110454771A CN 113511935 A CN113511935 A CN 113511935A
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- nutrient absorption
- absorption enhancer
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
Abstract
The invention discloses a plant nutrient absorption enhancer, and relates to the technical field of agriculture. The biological bactericide comprises, by weight, 40-60 parts of active substances, 15-20 parts of auxiliary substances, 10 parts of calcium nitrate, 10 parts of magnesium sulfate, 10 parts of ammonium dihydrogen phosphate and 5 parts of a biological bactericide. According to the invention, the active substances capable of improving the nutrient absorption capacity of different tissues of plant roots, stems and leaves are matched with a small amount of growth promoter to prepare the universal plant nutrient absorption promoter, so that the nutrient absorption capacity of the plant can be improved, the nutrient consumption is improved by taking growth and development as a guide, and the nutrient absorption of the plant is greatly improved; in addition, by adding a mixed biological agent consisting of bacillus subtilis and pararhizobium, and matching the bacillus subtilis and the pararhizobium, not only can a universal nutrient absorption enhancer be prepared, but also a nutrient absorption enhancer for special plants can be prepared, and the applicability of the plant nutrient absorption enhancer is further improved.
Description
Technical Field
The invention belongs to the technical field of agriculture, and particularly relates to a plant nutrient absorption enhancer.
Background
In agricultural production, in order to promote the growth of crops and increase the yield of crops, various fertilizers, including biomass fertilizers and artificially synthesized chemical fertilizers, are generally applied to soil to supplement various nutrient components required in the growth and development process of crops; however, in the process of plant growth and development, the plants are often influenced by various environmental factors, the fertilizing amount can directly change the structure of nutrient components in soil, and when the concentration of mineral nutrients in a medium exceeds the absorption capacity of crops, the absorption of the nutrient substances is reduced due to the feedback regulation of the plants, so that the nutrient components are accumulated on the surface layer of the soil to form fertilizer leaching loss, and underground water and surface water pollution is directly caused; on the other hand, since plants cannot absorb nutrients, the growth and development conditions of the plants are also affected, and the overall yield of crops is further reduced, the growth condition of the crops needs to be improved by applying an accelerant to the crops so as to improve the yield; however, most of the existing plant promoters are growth promoters, which cannot improve the current situation for crops which cannot absorb nutrition in soil, so that a plant nutrition absorption promoter is needed for promoting the crops to absorb nutrition ingredients in soil.
Disclosure of Invention
The invention aims to provide a plant nutrient absorption promoter, which solves the problems of environmental pollution and yield reduction caused by the reduction of nutrient absorption capability of plants such as the existing crops under the influence of the environment.
In order to solve the technical problems, the invention is realized by the following technical scheme:
the invention relates to a plant nutrient absorption enhancer which is an aqueous solution mainly containing active substances and having a pH value of 5.5-6.5, and comprises 40-60 parts by weight of active substances, 15-20 parts by weight of auxiliary substances, 10 parts by weight of calcium nitrate, 10 parts by weight of magnesium sulfate, 10 parts by weight of ammonium dihydrogen phosphate and 5 parts by weight of a biological agent;
the active substances comprise potassium polyaspartate and CEP polypeptide, wherein the molecular weight of the potassium polyaspartate is 5500-7500, the amino acid sequence of the CEP polypeptide is DFRPTAPGHSPGVGH, and the active substances are artificially synthesized by a solid phase polypeptide synthesis method (SPPS); among the two components in the active substance, the CEP polypeptide can promote the absorption of plant root systems to nitrate nitrogen when acting on plant roots and stems, so as to avoid the accumulation of nitrogen elements in soil, and the polyaspartic acid potassium is a bionic biopolymer with a unique molecular structure and is used as polypeptide protein containing potassium ions, so that when the polyaspartic acid potassium is applied to the plant roots, the potassium ions can be effectively drained and conveyed to plant cells for free potassium ions in the soil, and the absorption capacity of plants to the potassium ions is improved;
the auxiliary substances comprise 30-60% of indoleacetic acid and 40-70% of gibberellin by weight percentage, wherein the indoleacetic acid is a common plant growth promoter, can promote plant cell division and photosynthetic product distribution, enables leaves to be enlarged and seeds to germinate, and enables adventitious roots and lateral roots to be generated, on the other hand, the nutrient consumption of the plant can be improved, and the auxiliary substances are matched with polyaspartic acid potassium and CEP polypeptide to improve the nutrient absorption amount of plant roots; the gibberellin can break seed dormancy, improve plant setting rate or form seedless fruits, and can be mixed with acidic pesticides and fertilizers to improve the environmental adaptability of the plant nutrient absorption promoter;
the biological agent is a mixed agent of a bacillus subtilis agent and a crowtoe agent, wherein the bacillus subtilis agent is 60-80 wt% and the crowtoe agent is 20-40 wt%, wherein the crowtoe agent usually exists at rhizomes of leguminous plants to form a symbiotic system with the rhizomes of the leguminous plants, so that the yield is improved, the addition amount of the crowtoe agent is controlled to be 20-25% when the crowtoe agent is prepared and used according to the types of crops to be applied, namely when the crowtoe agent is applied to non-leguminous crops, and the addition amount is controlled to be 35-40% when the crowtoe agent is applied to leguminous crops; the bacillus subtilis can effectively accelerate the fermentation of humus in the cultivated land, further accelerate the fermentation of a biomass fertilizer and promote the absorption capacity of plants on nutrients in the fertilizer.
Further, the active substance is prepared into mixed mother liquor with the concentration of 1mol/L, wherein the mixed mother liquor comprises the potassium polyaspartate and the CEP polypeptide, and the solvent is buffer solution.
Furthermore, the buffer solution is a phosphate buffer solution, namely 0.2mol/L potassium dihydrogen phosphate solution is taken, 118ml of 0.2mol/L sodium hydroxide solution is added, ultrapure water is added to dilute the solution to 1000ml, and the pH value is adjusted to 6.0 by concentrated hydrochloric acid, so that the free state of the potassium polyaspartate can be stabilized, and the efficient absorption of potassium ions is ensured.
Furthermore, the auxiliary substances are prepared into a mixed solution with the concentration of 1mol/L and containing indoleacetic acid and gibberellin, the solvent is ultrapure water, the pH value is adjusted to 6.0 by concentrated hydrochloric acid, the pH value is consistent with the acid-base environment of the active substance mixed solution, and the subsequent preparation of the mixed solution is facilitated.
Furthermore, the colony number of the bacillus subtilis agent in the biological agent is 1.5 multiplied by 1011～2.5×1011The colony count of the crowfoot tumor microbial inoculum is 1.0 multiplied by 1011～1.5×1011One per gram.
Further, the biological agent is prepared into bacillus subtilis liquid and phlebia baimaiensis liquid with the same concentration.
Further, the preparation method of the plant nutrient absorption enhancer comprises the following steps: firstly, taking 50ml of active substance mixed mother liquor, adding 20ml of auxiliary substance mixed solution, and uniformly mixing; secondly, respectively mixing bacillus subtilis liquid and the sinorhizobium liquid according to the proportion of 4:1, adding a mixed solution of an active substance and an auxiliary substance, mainly applying to the preparation of a nutrient absorption enhancer or a universal nutrient absorption enhancer of non-leguminous plants, and changing the microbial inoculum proportion to 2:1 when the microbial inoculum is applied to leguminous plants; then 7.5g of calcium nitrate, 8.0g of magnesium sulfate and 10.0g of ammonium dihydrogen phosphate are respectively weighed and dissolved in a phosphate buffer solution, and the plant nutrient absorption enhancer is prepared by referring to the weight components of the plant nutrient absorption enhancer; and finally, adding the buffer solution of calcium nitrate, magnesium sulfate and ammonium dihydrogen phosphate into the mixed solution of the active substance and the auxiliary substance, uniformly mixing to prepare an accelerator solution, and sealing and refrigerating in a dark place.
Further, the application of the plant nutrient absorption enhancer is as follows: the accelerant solution is diluted by 600 times when soaking seeds, is diluted by 900 times when irrigating roots, and is diluted by 1200 times when spraying leaves.
The invention has the following beneficial effects:
according to the invention, the active substances capable of improving the nutrient absorption capacity of different tissues of plant roots, stems and leaves are matched with a small amount of growth promoter to prepare the universal plant nutrient absorption promoter, so that the nutrient absorption capacity of the plant can be improved, the nutrient consumption is improved by taking growth and development as a guide, and the nutrient absorption of the plant is greatly improved; in addition, by adding a mixed biological agent consisting of bacillus subtilis and pararhizobium, and matching the bacillus subtilis and the pararhizobium, not only can a universal nutrient absorption enhancer be prepared, but also a nutrient absorption enhancer for special plants can be prepared, and the applicability of the plant nutrient absorption enhancer is further improved.
Of course, it is not necessary for any product in which the invention is practiced to achieve all of the above-described advantages at the same time.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The invention relates to a plant nutrient absorption enhancer which is an aqueous solution mainly containing active substances and having a pH value of 5.5-6.5, and comprises 40-60 parts by weight of active substances, 15-20 parts by weight of auxiliary substances, 10 parts by weight of calcium nitrate, 10 parts by weight of magnesium sulfate, 10 parts by weight of ammonium dihydrogen phosphate and 5 parts by weight of a biological agent;
the active substances comprise potassium polyaspartate and CEP polypeptide, wherein the molecular weight of the potassium polyaspartate is 5500-7500, the amino acid sequence of the CEP polypeptide is DFRPTAPGHSPGVGH, and the potassium polyaspartate is artificially synthesized by a solid phase polypeptide synthesis method (SPPS); among the two components in the active substance, the CEP polypeptide can promote the absorption of plant root systems to nitrate nitrogen when acting on plant roots and stems, so as to avoid the accumulation of nitrogen elements in soil, and the polyaspartic acid potassium is a bionic biopolymer with a unique molecular structure and is used as polypeptide protein containing potassium ions, so that when the polyaspartic acid potassium is applied to the plant roots, the potassium ions can be effectively drained and conveyed to plant cells for free potassium ions in the soil, and the absorption capacity of plants to the potassium ions is improved;
the auxiliary substances comprise 30-60% of indoleacetic acid and 40-70% of gibberellin by weight percentage, wherein the indoleacetic acid is a common plant growth promoter, can promote plant cell division and photosynthetic product distribution, enables leaves to expand and seeds to germinate, and enables adventitious roots and lateral roots to generate, on the other hand, the auxiliary substances can improve the nutrient consumption of the plant per se, and can improve the nutrient absorption amount of the plant root system by matching with polyaspartic acid potassium and CEP polypeptide; the gibberellin can break seed dormancy, improve plant setting rate or form seedless fruits, and can be mixed with acidic pesticides and fertilizers to improve the environmental adaptability of the plant nutrient absorption promoter;
the biological agent is a mixed agent of a bacillus subtilis agent and a crow's foot agent, wherein the bacillus subtilis agent is 60-80 wt% and the crow's foot agent is 20-40 wt%, wherein the crow's foot agent usually exists at the root and stem of leguminous plants to form a symbiotic system with the roots and stems of the leguminous plants, so that the yield is improved, the addition amount of the crow's foot agent is controlled to be 20-25% when the crow's foot agent is prepared and used according to the types of crops to be applied, namely when the crow's foot agent is applied to non-leguminous crops, and the addition amount is controlled to be 35-40% when the crow's foot agent is applied to leguminous crops; the bacillus subtilis can effectively accelerate the fermentation of humus in the cultivated land, further accelerate the fermentation of a biomass fertilizer and promote the absorption capacity of plants on nutrients in the fertilizer.
Preferably, the active substance is prepared into a mixed mother liquor with the concentration of 1mol/L, wherein the mixed mother liquor comprises the potassium polyaspartate and the CEP polypeptide, and the solvent is a buffer solution.
Preferably, the buffer solution is phosphate buffer solution, namely 0.2mol/L potassium dihydrogen phosphate solution is taken, 118ml of 0.2mol/L sodium hydroxide solution is added, ultrapure water is added to dilute the solution to 1000ml, and the pH value is adjusted to 6.0 by concentrated hydrochloric acid, so that the free state of the potassium polyaspartate can be stabilized, and the efficient absorption of potassium ions is ensured.
Preferably, the auxiliary substance is prepared into a mixed solution with the concentration of 1mol/L and containing indoleacetic acid and gibberellin, the solvent is ultrapure water, the pH value is adjusted to 6.0 by concentrated hydrochloric acid, the pH value is consistent with the acid-base environment of the mixed solution of the active substance, and the subsequent preparation of the mixed solution is facilitated.
Preferably, the colony number of the bacillus subtilis agent in the biological agent is 1.5 multiplied by 1011～2.5×1011The colony count of the crowfoot tumor microbial inoculum is 1.0 multiplied by 1011～1.5×1011One per gram.
Preferably, the biological agent is prepared into bacillus subtilis liquid and phlebia baimaiensis liquid with the same concentration.
Preferably, the preparation method of the plant nutrient absorption enhancer comprises the following steps: firstly, taking 50ml of active substance mixed mother liquor, adding 20ml of auxiliary substance mixed solution, and uniformly mixing; secondly, respectively mixing bacillus subtilis liquid and the sinorhizobium liquid according to the proportion of 4:1, adding a mixed solution of an active substance and an auxiliary substance, mainly applying to the preparation of a nutrient absorption enhancer or a universal nutrient absorption enhancer of non-leguminous plants, and changing the microbial inoculum proportion to 2:1 when the microbial inoculum is applied to leguminous plants; then 7.5g of calcium nitrate, 8.0g of magnesium sulfate and 10.0g of ammonium dihydrogen phosphate are respectively weighed and dissolved in a phosphate buffer solution, and the plant nutrient absorption enhancer is prepared by referring to the weight components of the plant nutrient absorption enhancer; and finally, adding the buffer solution of calcium nitrate, magnesium sulfate and ammonium dihydrogen phosphate into the mixed solution of the active substance and the auxiliary substance, uniformly mixing to prepare an accelerator solution, and sealing and refrigerating in a dark place.
Preferably, the plant nutrient absorption enhancer is used as follows: the accelerant solution is diluted by 600 times when soaking seeds, is diluted by 900 times when irrigating roots, and is diluted by 1200 times when spraying leaves.
Example 2
The plant nutrient absorption accelerant prepared by the formula in the embodiment is used for carrying out nutrient absorption experiments in soilless culture of non-leguminous crop plants, the experimental object is culture solution of a soilless culture flask, the reference object is culture solution of the soilless culture flask without using the accelerant in the ratio, four groups of culture flasks used by the non-leguminous crop plants with the same growth condition are selected and respectively represented as an experimental group, a control group 1, a control group 2 and a control group 3, wherein the experimental group uses the plant nutrient absorption accelerant in the ratio, the control group 1 uses the local absorption accelerant for removing active substances, the control group 2 uses the local absorption accelerant for removing auxiliary substances, the control group 3 uses the local absorption accelerant for removing biological agents, and the plant nutrient absorption accelerant is used for root irrigation; after the same duration of use, the consumption of the culture solution by four bottles of cultivated plant plants is respectively detected to observe the nutrition absorption capacity of each group of plants, and the experimental results are shown in the following table:
table one shows the consumption of the culture solution in different time intervals after using the combination accelerator of each ratio
| Group of | 1 day | 2 days | 3 days | 4 days |
| Experimental group | 0.65mL | 1.2mL | 1.5mL | 1.65mL |
| Control group 1 | 0.55mL | 0.75mL | 0.85mL | 0.92mL |
| Control group 2 | 0.45mL | 0.66mL | 1.0mL | 1.05mL |
| Control group 3 | 0.53mL | 0.95mL | 1.1mL | 1.15mL |
As can be seen from the table I, the non-leguminous crop plants using the plant nutrient absorption enhancer of the invention have stronger nutrient absorption capacity when initially used, and then gradually tend to be gentle along with the time; after the active substances are removed, the absorption capacity is stronger when the plant is used for the first time, but the consumption of the plant to the nutrient components is gradually reduced; after the auxiliary substances are removed, although the absorption capacity is inferior to that of the control group 1 in the initial use, the absorption capacity tends to be stable after rapid increase; after the biological agent is removed, the whole trend of the nutrient absorption capacity of the plant is the same as that of an experimental group, but the peak value of the nutrient absorption capacity is lower than that of the experimental group, so that the whole capacity of the plant is not as good as that of the experimental group plant using the plant nutrient absorption enhancer prepared by the invention;
in conclusion, the plant nutrient absorption enhancer prepared according to the proportion is not only quick in action time, but also greatly improves the nutrient absorption capacity of crop plants after action, and is suitable for long-term popularization and use in agriculture.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (8)
1. A plant nutrient absorption enhancer is an aqueous solution mainly containing active substances and having a pH of 5.5-6.5, and is characterized in that: the biological bactericide comprises, by weight, 40-60 parts of active substances, 15-20 parts of auxiliary substances, 10 parts of calcium nitrate, 10 parts of magnesium sulfate, 10 parts of ammonium dihydrogen phosphate and 5 parts of a biological bactericide;
the active substances comprise potassium polyaspartate and CEP polypeptide, wherein the molecular weight of the potassium polyaspartate is 5500-7500, and the amino acid sequence of the CEP polypeptide is DFRPTAPGHSPGVGH;
the auxiliary substances comprise 30-60% of indoleacetic acid and 40-70% of gibberellin by weight percentage;
the biological agent is a mixed agent of a bacillus subtilis agent and a crowtoe agent, wherein the bacillus subtilis agent accounts for 60-80 wt% and the crowtoe agent accounts for 20-40 wt% of the mixed agent.
2. The plant nutrient absorption enhancer as claimed in claim 1, wherein the active substance is prepared as a mixed mother liquor comprising potassium polyaspartate and CEP polypeptide at a concentration of 1mol/L, and the solvent is a buffer solution.
3. The plant nutrient absorption enhancer as claimed in claim 1, wherein the buffer solution is phosphate buffer solution, which is prepared by adding 118ml of 0.2mol/L sodium hydroxide solution to 0.2mol/L potassium dihydrogen phosphate solution, diluting to 1000ml with ultrapure water, and adjusting pH to 6.0 with concentrated hydrochloric acid.
4. A plant nutrient absorption enhancer as claimed in claim 1, wherein the auxiliary substances are prepared as a mixed solution comprising indoleacetic acid and gibberellin at a concentration of 1mol/L, the solvent is ultrapure water, and the pH is adjusted to 6.0 with concentrated hydrochloric acid.
5. The plant nutrient absorption enhancer as claimed in claim 1, wherein the biological agent is a Bacillus subtilis agentThe number of colonies was 1.5X 1011～2.5×1011The colony count of the crowfoot tumor microbial inoculum is 1.0 multiplied by 1011～1.5×1011One per gram.
6. The plant nutrient absorption enhancer as claimed in claim 1, wherein the biological agent is prepared from a bacillus subtilis solution and a Lotus japonicus solution having the same concentration.
7. The plant nutrient absorption enhancer as claimed in claim 1, wherein the plant nutrient absorption enhancer is prepared by a method comprising: firstly, taking 50ml of active substance mixed mother liquor, adding 20ml of auxiliary substance mixed solution, and uniformly mixing; secondly, respectively mixing the bacillus subtilis liquid and the sinorhizobium baileyi liquid according to the proportion of 4:1, and adding a mixed solution of an active substance and an auxiliary substance; then 7.5g of calcium nitrate, 8.0g of magnesium sulfate and 10.0g of ammonium dihydrogen phosphate are respectively weighed and dissolved in a phosphate buffer solution; and finally, adding the buffer solution of calcium nitrate, magnesium sulfate and ammonium dihydrogen phosphate into the mixed solution of the active substance and the auxiliary substance, uniformly mixing to prepare an accelerator solution, and sealing and refrigerating in a dark place.
8. The plant nutrient absorption enhancer as claimed in claim 1, wherein the plant nutrient absorption enhancer is used in the form of: the accelerant solution is diluted by 600 times when soaking seeds, is diluted by 900 times when irrigating roots, and is diluted by 1200 times when spraying leaves.
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| CN202110454771.8A CN113511935A (en) | 2021-04-26 | 2021-04-26 | Plant nutrient absorption enhancer |
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| CN202110454771.8A CN113511935A (en) | 2021-04-26 | 2021-04-26 | Plant nutrient absorption enhancer |
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| CN112690290A (en) * | 2020-12-30 | 2021-04-23 | 阳田生物科技(江苏)有限公司 | Preparation method of bacillus subtilis plant growth promoter |
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2021
- 2021-04-26 CN CN202110454771.8A patent/CN113511935A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101379977A (en) * | 2007-09-07 | 2009-03-11 | 北京巨泰科技有限公司 | Plant nutrient absorption enhancer and preparation method and use thereof |
| CN103004764A (en) * | 2011-09-28 | 2013-04-03 | 北京盛大龙腾科技发展有限公司 | Plant nutrient absorption enhancer and application thereof |
| CN107473903A (en) * | 2017-09-29 | 2017-12-15 | 湖南省中科农业有限公司 | A kind of complete first biological organic fertilizer and preparation method thereof |
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Application publication date: 20211019 |