CN113480375B - Oil field mining area historical legacy oil-containing sludge microorganism induction in-situ advanced treatment method - Google Patents
Oil field mining area historical legacy oil-containing sludge microorganism induction in-situ advanced treatment method Download PDFInfo
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Abstract
The invention belongs to the technical field of sludge treatment, and particularly relates to an in-situ deep treatment method for historical legacy oily sludge microorganisms in an oil field mining area, which comprises the following steps: preparing lactobacillus bacteria liquid, bacillus bacteria liquid and petroleum degrading bacteria liquid, and mixing the bacteria liquids to obtain mixed bacteria liquid; mixing the mixed bacteria liquid with straw powder and a surfactant, and drying to prepare mixed bacteria-loaded bacteria powder; digging out the historical residual oily sludge of the well site, placing the oily sludge in the well site which is made with seepage prevention and leakage prevention in advance, and spreading the oily sludge; adding microbial powder, deep ploughing, spraying water on the ploughed soil surface, and standing for 3-7d; laying a layer of straw sections on the surface of soil, and fermenting for 20-30d; and (4) planting plants in the fermented soil, and supplementing the bacterial powder once every other month until the petroleum content in the soil meets the emission requirement. The invention combines microorganism and plant restoration, generates good petroleum degradation effect and has better application prospect.
Description
Technical Field
The invention belongs to the technical field of sludge treatment, and particularly relates to an in-situ deep treatment method for historical legacy oily sludge microorganisms in an oil field mining area.
Background
The historical oily sludge of the oil field mining area/well site is a mining area historical residual environment problem which is comprehensively formed in the early stage of the oil field development process due to the influence of technical process limitation and environmental protection attention degree, wherein petroleum hydrocarbon and chemical agent residues cause great risk to the mining area environment, and along with the improvement of the oil field development technical level and the increase of the strength of China in the environmental management of the mining area, the historical oily sludge of the oil field mining area/well site must be cleared and treated, the ecological function of polluted soil is recovered, and the harmonious symbiosis of the oil field high-quality development and the mining area environment is realized. In the prior art.
The microorganism treatment method is a common oil-containing sludge treatment method at present, and selects microorganisms with the function of degrading organic matters, such as pseudomonas, rhodococcus, micrococcus, bacillus and the like with petroleum degradation capacity, wherein the pseudomonas is a straight or slightly bent gram-negative bacillus, has no cell nucleus, moves by polar flagella and does not form spores; the rhodococcus is a kind of aerobic gram-positive bacteria, can form a rudiment mycelium, does not move, and has rough or smooth bacterial colony; micrococcus is a gram-positive bacterium with spherical cells, rarely moves, does not produce spores, and has smooth bacterial colony; bacillus is a genus that produces spores that are particularly resistant to adverse conditions, and is resistant to many adverse environments. The microorganisms have good petroleum hydrocarbon degradation capability after being singly or mixed, and can be used for bioremediation of petroleum sludge or petroleum polluted soil.
In the prior art, there are many technologies for degrading organic pollutants by using microorganisms, for example, chinese patent CN108580539A discloses a method for restoring organic polluted soil by coupling chemical oxidation with microorganisms, which sequentially adds ferrous sulfate activator solution, sodium persulfate oxidant solution and potassium permanganate oxidant solution into the soil to be restored, and changes the community structure of the soil microorganisms by using chemical reagents, so as to realize in-situ efficient economic restoration of the organic polluted soil; for example, the research on influence factors of the fixed microorganism for repairing the petroleum-polluted soil [ Zhang Xiuxia, baixuejing, xuna, and the like ], environmental engineering report, 2013 (03): 1156-1162' discloses a method for degrading the petroleum-polluted soil by utilizing the efficient petroleum-degrading single bacterium SM-3, which takes a natural organic material as a carrier, prepares the fixed microorganism by an adsorption method, then applies the free and fixed microorganisms to an indoor flowerpot, simulates and repairs the petroleum-polluted soil, and the result shows that the degradation rate of the fixed single bacterium SM-3 is 22.77% after 21d of repair. These studies all show the function of microorganisms in degrading petroleum pollutants.
Although the method for degrading petroleum pollutants by microorganisms is long in time consumption, the operation is simple, the energy consumption of equipment is low, and new pollution is not easily caused, so that the method becomes a better sludge treatment mode, but a plurality of microorganism functions are degraded, and when the use times of the microorganism are more, the degradation capability of organic matters is reduced, so that a new method for treating sludge by microorganisms needs to be continuously developed.
Disclosure of Invention
In order to solve the technical problems, the invention provides a method for the microorganism-induced in-situ advanced treatment of the historical residual oily sludge in the mining area of the oil field, and the method is a novel technology for the microorganism-induced in-situ advanced treatment of the oily sludge.
The invention aims to provide a microbial induction in-situ advanced treatment method for historical residual oily sludge in an oil field mining area, which comprises the following steps:
s1, preparing bacterial powder:
preparing lactobacillus bacteria liquid, bacillus bacteria liquid and petroleum degrading bacteria liquid, and mixing the bacteria liquids to obtain mixed bacteria liquid;
mixing the mixed bacteria liquid with straw powder and a surfactant, and drying to prepare the mixed bacteria-loaded straw powder for later use;
s2, in-situ sludge transfer: digging out the historical residual oily sludge of the well site, placing the dug oily sludge in the well site with seepage prevention and leakage prevention and 50cm high cofferdams at the periphery, and spreading the dug oily sludge;
s3, primary fermentation: uniformly scattering bacterial powder on the soil surface for deep ploughing, sprinkling water on the soil surface after deep ploughing, and standing for 3-7d;
s4, post-fermentation: laying a layer of straw sections on the surface of soil, and fermenting for 20-30d;
s5, phytoremediation: and (4) planting plants in the fermented soil, and supplementing the bacterial powder once every other month until the petroleum content in the soil meets the emission requirement.
Preferably, the oil field mining area history legacy oily sludge microorganism induction in-situ deep treatment method comprises the step of carrying out deep treatment on the oil field mining area history legacy oily sludge by using lactobacillus reuteri CGMCC NO.15528, the bacillus is bacillus megaterium CGMCC NO.3770, and the petroleum degrading bacteria are one or two of BDB-n biodegradable bacteria and BDB-a biodegradable bacteria.
Preferably, in the oil field mining area history remaining oily sludge microorganism induction in-situ advanced treatment method, the number of viable bacteria in the lactic acid bacteria liquid, the bacillus liquid and the petroleum degrading bacteria liquid is more than 107/mL, and the mass ratio of the lactic acid bacteria liquid, the bacillus liquid and the petroleum degrading bacteria liquid in the mixed bacteria liquid is 1.
Preferably, in the method for deep treatment of the oil-containing sludge historical remnants in the oilfield, in step S2, the spreading and drying thickness of the oil-containing sludge in the wellsite field is 10-25cm, and the plowing depth is 25-40cm.
Preferably, in the method for the microbial-induced in-situ advanced treatment of the historical residual oily sludge in the oil field mining area, in S3, the addition amount of the bacterial powder is 5-10kg per cubic meter of the oily sludge, and the water spraying amount is 1-2L per cubic meter of the oily sludge.
Preferably, the oil field mining area history legacy oily sludge microorganism induction in-situ advanced treatment method is adopted, and the straw is one or more of corn straw, wheat straw, sorghum straw and rice straw.
Preferably, in the oil field mining area history residual oily sludge microorganism induction in-situ advanced treatment method, the straw powder is obtained by crushing straws into straw powder with the length of 2-5 mm;
the straw sections are straw sections with the length of 2-5cm, and the laying thickness of the straw sections on the soil surface is 5-10m.
Preferably, in the method for the microbial-induced in-situ advanced treatment of the oily sludge left in the history of the oilfield mining area, the bacterial powder is prepared from a mixed bacterial liquid: straw powder: the surfactant is prepared by mixing the following components in a mass ratio of 100:
weighing the mixed bacteria liquid, the straw powder and the surfactant according to a proportion, dividing the surfactant into two parts with equal mass, fully mixing one part of the surfactant with the mixed bacteria liquid to obtain a bacteria liquid mixture, mixing the other part of the surfactant with the straw powder to obtain a straw mixture, mixing the bacteria liquid mixture with the straw mixture, and drying to obtain the bacteria powder.
Preferably, the oil field mining area history residual oily sludge microorganism induction in-situ advanced treatment method is adopted, and the surfactant is glycerol.
Preferably, in the method for performing in-situ deep treatment on historical residual oily sludge in an oil field mining area by microorganism induction, in step S5, the plant is a mixed seed obtained by mixing corn grass, ryegrass or green bristlegrass seeds according to a mass ratio of 1; the addition amount of the fungus powder is 0.2-0.5 times of the previous fungus powder.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention selects lactobacillus reuteri CGMCC NO.15528, bacillus megaterium CGMCC NO.3770, BDB-n biodegradable bacteria and BDB-a biodegradable bacteria with petroleum degradation function, combines the microorganisms with plant repair, generates good petroleum degradation effect and has better application prospect.
2. The method disperses redundant petroleum pollutants in the oily sludge by utilizing the viscosity of the soil in the well site field, so that the content of petroleum in the sludge is reduced; soil in the well site field provides a nutrient medium for the growth of subsequent plants; standing for 3-7 days during primary fermentation to allow the microorganisms to penetrate into the soil and propagate, wherein the microorganisms have a primary degradation effect on oil dirt in the soil; during post-fermentation, straw sections are added for fermentation for 20-30d, the straw sections have the functions of preserving heat and moisture and providing proper temperature and humidity for fermentation of microorganisms, and the straws after being decomposed by the microorganisms can improve soil quality and increase the content of soil nutrient elements; the microbial powder is added in the plant repairing process, and the petroleum degradation effect can be enhanced together with the plant.
3. The invention designs a special preparation method of microbial bacteria powder, which comprises the steps of dividing a surfactant into two parts with equal mass, fully mixing one part of the surfactant with a mixed bacterial liquid to obtain a bacterial liquid mixture, mixing the other part of the surfactant with straw powder to obtain a straw mixture, changing the surface polarity of the mixed bacterial liquid added with the surfactant and the straw powder, and mixing the bacterial liquid mixture with the straw mixture, wherein bacteria can be quickly adsorbed on the straw powder.
4. The petroleum sludge treated by the method can be used as a base fertilizer or a soil improvement matrix for improving the soil nutrition of barren fields, and the planted herbaceous crops can be used as greening environments.
Drawings
FIG. 1 is a flow chart of the method for the microorganism-induced in-situ advanced treatment of the historical residual oily sludge in the mining area of the oil field.
Detailed Description
In order that those skilled in the art will better understand the technical solutions of the present invention to be implemented, the present invention will be further described with reference to the following specific embodiments and accompanying drawings.
In the description of the present invention, reagents used are commercially available and methods used are conventional in the art, unless otherwise specified. The lactobacillus reuteri CGMCC NO.15528 and the bacillus megaterium CGMCC NO.3770 are purchased from China general microbiological culture Collection center (CGMCC); the BDB-n biodegradable bacteria and the BDB-a biodegradable bacteria are sold in the market and are products developed by the south China Donghua company and the Beijing university college of environmental college; the above-mentioned method for the expanded culture of each bacterial suspension was carried out by a conventional method, or commercially available bacterial suspensions were directly purchased. The oily sludge used in the invention is from the well site of an oil field for prolonging petroleum, and the heavy metal content in the sludge-containing sample used in the invention meets the agricultural sludge pollutant control standard GB4284-2018.
It should be noted that, in the present invention, all the test data are randomly sampled and measured in three times and expressed in the form of "mean ± standard deviation".
The invention provides a microbial-induced in-situ advanced treatment method for historical residual oily sludge in an oil field mining area, which comprises the following embodiments.
Example 1
An in-situ deep treatment method for historical legacy oily sludge microorganisms in an oil field mining area comprises the following steps:
s1, preparing a lactobacillus bacterial liquid, a bacillus bacterial liquid and a petroleum degrading bacterial liquid, and mixing the bacteria liquids to obtain a mixed bacterial liquid for later use; the lactobacillus is Lactobacillus reuteri CGMCC NO.15528, and the concentration of the bacteria liquid is 3 x 10 7 Each mL, the bacillus is bacillus megaterium CGMCC NO.3770, and the concentration of the bacterial liquid is 6 multiplied by 10 7 The oil degrading bacteria are BDB-n biodegradation bacteria, and the concentration of the bacteria liquid is 5 multiplied by 10 7 Per mL; the mass ratio of the lactobacillus bacterial liquid, the bacillus bacterial liquid and the petroleum degrading bacterial liquid is (1);
the bacterial powder is prepared from mixed bacterial liquid: straw powder: the surfactant is prepared by mixing the following components in a mass ratio of 100:
weighing the mixed bacteria liquid, the straw powder and the surfactant according to a ratio, dividing the surfactant into two parts with equal mass, fully mixing one part of the surfactant with the mixed bacteria liquid to obtain a bacteria liquid mixture, mixing the other part of the surfactant with the straw powder to obtain a straw mixture, fully mixing the bacteria liquid mixture with the straw mixture, and carrying out ventilation drying at 30 ℃ to obtain bacteria powder;
the straw powder is formed by crushing dried wheat straws into straw powder with the length of 2 mm; the surfactant is glycerol;
s2, in-situ sludge transfer: will be 20m 3 Digging out the historical oily sludge left in the well site, placing the well site in a well site field with seepage prevention and leakage prevention and 50cm high cofferdams at the periphery, spreading out, and spreading and drying the oily sludge on the soil to be 15cm in thickness;
s3, primary fermentation: uniformly scattering 100kg of bacterial powder on the surface of soil, and deeply ploughing once, wherein the ploughing depth is 25cm; the plowed sludge is basically and uniformly mixed with the field soil of the well site, and the test shows that the mass fraction of oil in the mixed soil with the depth of 10cm is 8.67 +/-0.29 g/100g; spraying 20L of water on the surface of the deeply ploughed soil, and standing for 3d;
s4, post-fermentation: laying a layer of straw sections with the thickness of 5cm on the surface of soil, and fermenting for 30d; the straw section is formed by crushing dried wheat straws into straw sections with the length of 5cm; sampling and testing the oil content in the soil with the depth of 10cm to be 3.72 +/-0.07 g/100g, and the oil removal rate is ((8.67-3.72)/8.67) × 100% =57.09%;
s5, phytoremediation: and (3) planting plants in the fermented soil, uniformly spraying 20kg of bacterial powder every other month, and sampling and testing the oil content of the soil with the depth of 10cm to be 1.12 +/-0.19 g/100g and the oil removal rate to be ((8.67-1.12)/8.67). Times.100% =87.08% after one month.
Example 2
An in-situ deep treatment method for historical legacy oily sludge microorganisms in an oil field mining area comprises the following steps:
s1, preparing a lactobacillus bacterial liquid, a bacillus bacterial liquid and a petroleum degrading bacterial liquid, and mixing the bacteria liquids to obtain a mixed bacterial liquid for later use; the lactobacillus is Lactobacillus reuteri CGMCC NO.15528, and the concentration of the bacteria liquid is 3 x 10 7 Per mL, the bacillus is bacillus megaterium CGMCCNo.3770, bacterial liquid concentration is 6X 10 7 The oil degrading bacteria are BDB-a biodegradable bacteria, and the concentration of the bacteria liquid is 6 multiplied by 10 7 Per mL; the mass ratio of the lactobacillus bacterial liquid to the bacillus bacterial liquid to the petroleum degrading bacterial liquid is 1;
the bacterial powder is prepared from mixed bacterial liquid: straw powder: the surfactant is prepared by mixing the following components in a mass ratio of 100:
weighing the mixed bacteria liquid, the straw powder and the surfactant according to a ratio, dividing the surfactant into two parts with equal mass, fully mixing one part of the surfactant with the mixed bacteria liquid to obtain a bacteria liquid mixture, mixing the other part of the surfactant with the straw powder to obtain a straw mixture, fully mixing the bacteria liquid mixture with the straw mixture, and carrying out ventilation drying at 30 ℃ to obtain bacteria powder;
the straw powder is straw powder which is formed by crushing dried corn straws and has the length of 5 mm; the surfactant is glycerol;
s2, in-situ sludge transfer: 20m is put 3 Digging out the oil-containing sludge which is historically left in the well site, placing the oil-containing sludge in the well site with the functions of seepage prevention, leakage prevention and 50cm high cofferdams at the periphery, and spreading the oil-containing sludge out of the soil to a spreading thickness of 25cm;
s3, primary fermentation: uniformly scattering 200kg of bacterial powder on the surface of soil, and deeply ploughing once, wherein the ploughing depth is 40cm; the plowed sludge is basically and uniformly mixed with the well site field soil, the test shows that the mass fraction of oil in the mixed soil with the depth of 10cm is 6.43 +/-0.19 g/100g, 40L of water is sprayed on the surface of the deeply plowed soil, and then the mixture is kept still for 7d;
s4, post-fermentation: laying a layer of straw sections with the thickness of 10cm on the surface of soil, and fermenting for 20d; the straw section is formed by crushing dried corn straws into straw sections with the length of 2 cm; sampling and testing the oil content in the soil with the depth of 10cm to be 3.57 +/-0.10 g/100g, and the oil removal rate is ((6.43-3.57)/6.43) × 100% =44.48%;
s5, phytoremediation: planting plants in the fermented soil, uniformly spraying 60kg of bacterial powder every other month, and after one month, sampling and testing the oil content in the soil with the depth of 10cm to be 0.84 +/-0.04 g/100g, wherein the oil removal rate is ((6.43-0.84)/6.43) × 100% =86.94%.
Example 3
An in-situ deep treatment method for historical legacy oily sludge microorganisms in an oil field mining area comprises the following steps:
s1, preparing a lactobacillus bacterial liquid, a bacillus bacterial liquid and a petroleum degrading bacterial liquid, and mixing the bacteria liquids to obtain a mixed bacterial liquid for later use; the lactobacillus is Lactobacillus reuteri CGMCC NO.15528, and the concentration of the bacteria liquid is 3 x 10 7 The bacillus is bacillus megatherium CGMCC NO.3770, the concentration of the bacterial liquid is 6 multiplied by 10 7 The petroleum degrading bacteria are a mixture of BDB-n biodegradation bacteria and BDB-a biodegradation bacteria according to the mass ratio of 1 7 The bacterial concentration of the BDB-a biodegradable bacteria is 6 multiplied by 10 7 Per mL; the mass ratio of the lactobacillus bacterial liquid to the bacillus bacterial liquid to the petroleum degrading bacterial liquid is 1;
the bacterial powder is prepared from mixed bacterial liquid: straw powder: the surfactant is prepared by mixing the following components in a mass ratio of 100:
weighing the mixed bacteria liquid, the straw powder and the surfactant according to a ratio, dividing the surfactant into two parts with equal mass, fully mixing one part of the surfactant with the mixed bacteria liquid to obtain a bacteria liquid mixture, mixing the other part of the surfactant with the straw powder to obtain a straw mixture, fully mixing the bacteria liquid mixture with the straw mixture, and carrying out ventilation drying at 30 ℃ to obtain bacteria powder;
the straw powder is made by crushing dry sorghum straws into straw powder with the length of 4 mm; the surfactant is glycerol;
s2, in-situ sludge transfer: will be 20m 3 Digging out the oil-containing sludge which is historically left in the well site, placing the oil-containing sludge in the well site with the functions of seepage prevention, leakage prevention and 50cm high cofferdams at the periphery, and spreading out the oil-containing sludge, wherein the spreading thickness of the oil-containing sludge on the soil is 10cm;
s3, primary fermentation: uniformly scattering 140kg of bacterial powder on the surface of soil, and deeply ploughing once, wherein the ploughing depth is 25cm; the plowed sludge is basically and uniformly mixed with the field soil of the well site, and the test shows that the mass fraction of oil in the mixed soil with the depth of 10cm is 8.30 +/-0.16 g/100g; spraying 30L of water on the surface of the deeply ploughed soil, and then standing for 5d;
s4, post-fermentation: laying a layer of straw sections with the thickness of 5cm on the soil surface, and fermenting for 25d; the straw sections are straw sections obtained by crushing dried sorghum straws into 5cm in length; the oil content in soil with a sampling test depth of 10cm is 4.00 +/-0.12 g/100g, and the oil removal rate is ((8.30-4.00)/8.30) × 100% =51.80%;
s5, phytoremediation: and planting plants in the fermented soil, uniformly spraying 42kg of bacterial powder every other month, and sampling and testing the oil content of the soil with the depth of 10cm to be 0.94 +/-0.03 g/100g and the oil removal rate to be ((8.30-0.94)/8.30). Times.100% =88.67% after one month.
Example 4
An in-situ induction advanced treatment method for historical legacy oily sludge microorganisms in an oil field mining area comprises the following steps:
s1, preparing a lactobacillus bacterial liquid, a bacillus bacterial liquid and a petroleum degrading bacterial liquid, and mixing the bacteria liquids to obtain a mixed bacterial liquid for later use; the lactobacillus is Lactobacillus reuteri CGMCC NO.15528, and the concentration of the bacteria liquid is 3 × 10 7 Each mL, the bacillus is bacillus megaterium CGMCC NO.3770, and the concentration of the bacterial liquid is 6 multiplied by 10 7 each/mL, the petroleum degrading bacteria are BDB-n biodegradable bacteria, and the concentration of the bacteria liquid is 5 multiplied by 10 7 Per mL; the mass ratio of the lactobacillus bacterial liquid, the bacillus bacterial liquid and the petroleum degrading bacterial liquid is (1);
the bacterial powder is prepared from mixed bacterial liquid: straw powder: the surfactant is prepared by mixing the following components in a mass ratio of 100:
weighing mixed bacteria liquid, straw powder and a surfactant according to a ratio, dividing the surfactant into two parts with equal mass, fully mixing one part of the surfactant with the mixed bacteria liquid to obtain a bacteria liquid mixture, mixing the other part of the surfactant with the straw powder to obtain a straw mixture, fully mixing the bacteria liquid mixture with the straw mixture, and carrying out ventilation drying at 30 ℃ to obtain bacteria powder;
the straw powder is prepared by crushing dry rice straws into straw powder with the length of 2 mm; the surfactant is glycerol;
s2, in-situ sludge transfer: will be 20m 3 Digging out the historical oily sludge left in the well site, placing the well site in a well site field with seepage prevention and leakage prevention and 50cm high cofferdams at the periphery, spreading out, and spreading and drying the oily sludge on the soil to be 15cm in thickness;
s3, primary fermentation: uniformly scattering 200kg of bacterial powder on the surface of soil, and deeply ploughing once, wherein the ploughing depth is 25cm; the plowed sludge is basically and uniformly mixed with the field soil of the well site, and the test shows that the mass fraction of oil in the mixed soil with the depth of 10cm is 8.19 +/-0.26 g/100g; spraying 20L of water on the surface of the deeply ploughed soil, and standing for 3d;
s4, post-fermentation: laying a layer of straw sections with the thickness of 5cm on the surface of soil, and fermenting for 30d; the straw sections are straw sections with the length of 5cm which are obtained by crushing dry rice straws; sampling and testing the oil content in the soil with the depth of 10cm to be 3.48 +/-0.06 g/100g, and the oil removal rate is ((8.19-3.48)/8.19) × 100% =57.51%;
s5, phytoremediation: planting plants in the fermented soil, uniformly spraying 100kg of bacterial powder every other month, and after one month, sampling and testing the oil content in the soil with the depth of 10cm to be 0.76 +/-0.03 g/100g, wherein the oil removal rate is ((8.19-0.76)/8.19). Times.100% =90.72%.
Experimental example 1
An oil field mining area historical legacy oily sludge microorganism induction in-situ advanced treatment method comprises the following steps:
s1, preparing lactobacillus bacterial liquid for later use; the lactobacillus is Lactobacillus reuteri CGMCC NO.15528, and the concentration of the bacteria liquid is 3 × 10 7 The amount of lactobacillus in the experimental example is the same as that in the example 1, and the bacteria liquid of the bacillus and the petroleum degrading bacteria in the example 1 is replaced by sterile water with equal mass;
the bacterium powder is prepared from lactobacillus bacterium liquid: straw powder: the surfactant is prepared by mixing the following components in a mass ratio of 100:
weighing the mixed bacteria liquid, the straw powder and the surfactant according to a ratio, dividing the surfactant into two parts with equal mass, fully mixing one part of the surfactant with the mixed bacteria liquid to obtain a bacteria liquid mixture, mixing the other part of the surfactant with the straw powder to obtain a straw mixture, fully mixing the bacteria liquid mixture with the straw mixture, and carrying out ventilation drying at 30 ℃ to obtain bacteria powder;
the straw powder is straw powder with the length of 2mm which is formed by crushing dry wheat straws; the surfactant is glycerol;
s2, in-situ sludge transfer: 20m is put 3 Digging out the oil-containing sludge which is historically left in the well site, placing the oil-containing sludge in the well site with the functions of seepage prevention, leakage prevention and 50cm high cofferdams at the periphery, and spreading out the oil-containing sludge, wherein the spreading thickness of the oil-containing sludge on the soil is 15cm;
s3, primary fermentation: uniformly scattering 100kg of bacterial powder on the surface of soil, and deeply ploughing for one time, wherein the ploughing depth is 25cm; the plowed sludge is basically and uniformly mixed with the field soil of the well site, and the test shows that the mass fraction of oil in the mixed soil with the depth of 10cm is 8.75 +/-0.23 g/100g; spraying 20L of water on the surface of the deeply ploughed soil, and standing for 3d;
s4, post-fermentation: laying a layer of straw sections with the thickness of 5cm on the surface of soil, and fermenting for 30d; the straw sections are straw sections obtained by crushing dried wheat straws into 5cm in length; sampling and testing the oil content in the soil with the depth of 10cm to be 8.00 +/-0.16 g/100g, and the oil removal rate is ((8.75-8.00)/8.75) × 100% =8.57%;
s5, phytoremediation: and (3) planting plants in the fermented soil, uniformly spraying 20kg of bacterial powder every other month, and after one month, sampling and testing the oil content in the soil with the depth of 10cm to be 7.52 +/-0.13 g/100g, wherein the oil removal rate is ((8.75-7.52)/8.75) multiplied by 100% =14.06%.
Experimental example 2
An oil field mining area historical legacy oily sludge microorganism induction in-situ advanced treatment method comprises the following steps:
s1, preparing a bacillus liquid for later use; the bacillus is bacillus megaterium CGMCC NO.3770, and the concentration of the bacterial liquid is 6 multiplied by 10 7 Each mL, the using amount of the lactic acid bacteria in the experimental example is the same as that of the lactic acid bacteria in the example 1, and sterile water with equal mass is used for replacing the lactic acid bacteria and the petroleum degrading bacteria liquid in the example 1;
the bacterial powder is prepared from bacillus bacterial liquid: straw powder: the surfactant is prepared by mixing the following components in a mass ratio of 100:
weighing the mixed bacteria liquid, the straw powder and the surfactant according to a ratio, dividing the surfactant into two parts with equal mass, fully mixing one part of the surfactant with the mixed bacteria liquid to obtain a bacteria liquid mixture, mixing the other part of the surfactant with the straw powder to obtain a straw mixture, fully mixing the bacteria liquid mixture with the straw mixture, and carrying out ventilation drying at 30 ℃ to obtain bacteria powder;
the straw powder is straw powder with the length of 2mm which is formed by crushing dry wheat straws; the surfactant is glycerol;
s2, in-situ sludge transfer: 20m is put 3 Digging out the oil-containing sludge which is historically left in the well site, placing the oil-containing sludge in the well site with the functions of seepage prevention, leakage prevention and 50cm high cofferdams at the periphery, and spreading out the oil-containing sludge, wherein the spreading thickness of the oil-containing sludge on the soil is 15cm; (ii) a
S3, primary fermentation: uniformly scattering 100kg of bacterial powder on the surface of soil, and deeply ploughing once, wherein the ploughing depth is 25cm; the plowed sludge is basically and uniformly mixed with the well site soil, and the mass fraction of oil in the mixed soil with the depth of 10cm is 8.45 +/-0.39 g/100g through testing; spraying 20L of water on the surface of the deeply ploughed soil, and standing for 3d;
s4, post-fermentation: laying a layer of straw sections with the thickness of 5cm on the soil surface, and fermenting for 30d; the straw section is formed by crushing dried wheat straws into straw sections with the length of 5cm; the oil content in soil with a sampling test depth of 10cm is 7.82 +/-0.09 g/100g, and the oil removal rate is ((8.45-7.82)/8.45) × 100% =7.46%;
s5, phytoremediation: and (3) planting plants in the fermented soil, uniformly spraying 20kg of bacterial powder every other month, and after one month, sampling and testing the oil content in the soil with the depth of 10cm to be 7.03 +/-0.06 g/100g, wherein the oil removal rate is ((8.45-7.03)/8.45). Times.100% =16.80%.
Experimental example 3
An in-situ deep treatment method for historical legacy oily sludge microorganisms in an oil field mining area comprises the following steps:
s1, preparing a petroleum degrading bacterium liquid for later use; the petroleum degrading bacteria are BDB-n biodegradable bacteria, and the concentration of the bacteria liquid is 5 multiplied by 10 7 Per mL; in the experimental example, the amount of the petroleum degrading bacteria is the same as that of the petroleum degrading bacteria in the example 1, and sterile water with equal mass is used for replacing the lactobacillus bacteria liquid and the bacillus bacteria liquid in the example 1;
the bacterial powder is prepared from bacillus bacterial liquid: straw powder: the surfactant is prepared by mixing the following components in a mass ratio of 100:
weighing the mixed bacteria liquid, the straw powder and the surfactant according to a ratio, dividing the surfactant into two parts with equal mass, fully mixing one part of the surfactant with the mixed bacteria liquid to obtain a bacteria liquid mixture, mixing the other part of the surfactant with the straw powder to obtain a straw mixture, fully mixing the bacteria liquid mixture with the straw mixture, and carrying out ventilation drying at 30 ℃ to obtain bacteria powder;
the straw powder is formed by crushing dried wheat straws into straw powder with the length of 2 mm; the surfactant is glycerol;
s2, in-situ sludge transfer: will be 20m 3 Digging out the historical oily sludge left in the well site, placing the well site in a well site field with seepage prevention and leakage prevention and 50cm high cofferdams at the periphery, spreading out, and spreading and drying the oily sludge on the soil to be 15cm in thickness;
s3, primary fermentation: uniformly scattering 100kg of bacterial powder on the surface of soil, and deeply ploughing once, wherein the ploughing depth is 25cm; (ii) a The plowed sludge is basically and uniformly mixed with the well site soil, and the mass fraction of oil in the mixed soil with the depth of 10cm is 8.33 +/-0.11 g/100g through testing; spraying 20L of water on the surface of the deeply ploughed soil, and standing for 3d;
s4, post-fermentation: laying a layer of straw sections with the thickness of 5cm on the soil surface, and fermenting for 30d; the straw section is formed by crushing dried wheat straws into straw sections with the length of 5cm; sampling and testing the oil content in soil with the depth of 10cm to be 6.51 +/-0.15 g/100g, and the oil removal rate is ((8.33-6.51)/8.33) × 100% =21.85%;
s5, phytoremediation: and (3) planting plants in the fermented soil, uniformly spraying 20kg of bacterial powder every other month, and after one month, sampling and testing the oil content in the soil with the depth of 10cm to be 4.29 +/-0.15 g/100g, wherein the oil removal rate is ((8.33-4.29)/8.33) × 100% =48.50%.
In the above examples and experimental examples, the oil content of the soil in the depth of 10cm in the plowed sludge is different from that of the soil in the well site field, and is influenced by the plowing depth and the oil content of the soil in the well site field.
It should be noted that, when the present invention relates to a numerical range, it should be understood that two endpoints of each numerical range and any value between the two endpoints can be selected, and since the steps and methods adopted are the same as those in the embodiment, in order to prevent redundancy, the present invention describes a preferred embodiment. While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. Therefore, it is intended that the appended claims be interpreted as including the preferred embodiment and all changes and modifications that fall within the scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (9)
1. The oil field mining area historical legacy oily sludge microorganism induction in-situ advanced treatment method is characterized by comprising the following steps:
s1, preparing bacterial powder:
preparing lactobacillus bacteria liquid, bacillus bacteria liquid and petroleum degrading bacteria liquid, and mixing the bacteria liquids to obtain mixed bacteria liquid; the lactobacillus is Lactobacillus reuteri CGMCC NO.15528, the bacillus is Bacillus megaterium CGMCC NO.3770, and the petroleum degrading bacteria are one or two of BDB-n biodegradable bacteria and BDB-a biodegradable bacteria;
mixing the mixed bacteria liquid with straw powder and a surfactant, and drying to prepare the mixed bacteria-loaded straw powder for later use;
s2, in-situ sludge transfer: digging out the historical residual oily sludge of the well site, placing the dug oily sludge in the well site with seepage prevention and leakage prevention and 50cm high cofferdams at the periphery, and spreading the dug oily sludge;
s3, primary fermentation: uniformly scattering bacterial powder on the surface of the soil for deep ploughing, spraying water on the surface of the soil after deep ploughing, and standing for 3-7d;
s4, post-fermentation: laying a layer of straw sections on the soil surface, and fermenting for 20-30d;
s5, phytoremediation: and (4) planting plants in the fermented soil, and supplementing the bacterial powder once every other month until the petroleum content in the soil meets the emission requirement.
2. The method for the in-situ deep treatment of the oil field mining area historical residual oily sludge through microorganism induction according to claim 1, wherein the viable count of the lactic acid bacteria liquid, the bacillus liquid and the petroleum degrading bacteria liquid is 10 7 More than one/mL, wherein the mass ratio of the lactobacillus bacterial liquid to the bacillus bacterial liquid to the petroleum degrading bacterial liquid in the mixed bacterial liquid is 1.
3. The method for the microorganism-induced in-situ deep treatment of the oily sludge left in the historical oilfield mining area according to claim 2, wherein in S2, the spreading and drying thickness of the oily sludge in the wellsite field is 10-25cm, and the plowing depth is 25-40cm.
4. The method for the microbial-induced in-situ advanced treatment of the historical residual oily sludge in the oilfield mining area according to claim 3, wherein in S3, the addition amount of the bacterial powder is 5-10kg per cubic meter of the oily sludge, and the water spraying amount is 1-2L per cubic meter of the oily sludge.
5. The method for the microbial-induced in-situ advanced treatment of the historical residual oily sludge in the oilfield mining area according to claim 4, wherein the straws are one or more of corn straws, wheat straws, sorghum straws and rice straws.
6. The method for the microbial-induced in-situ advanced treatment of the oil-containing sludge historically left in the oil field mining area according to claim 5, wherein the straw powder is prepared by crushing straws into straw powder with the length of 2-5 mm;
the straw sections are straw sections with the length of 2-5cm, and the laying thickness of the straw sections on the soil surface is 5-10m.
7. The method for the microbial-induced in-situ advanced treatment of the historical legacy oily sludge in the oilfield mining area according to claim 6, wherein the bacterial powder is prepared by mixing bacterial liquid: straw powder: the surfactant is prepared by mixing the following components in a mass ratio of 100:
weighing the mixed bacteria liquid, the straw powder and the surfactant according to a ratio, dividing the surfactant into two parts with equal mass, fully mixing one part of the surfactant with the mixed bacteria liquid to obtain a bacteria liquid mixture, mixing the other part of the surfactant with the straw powder to obtain a straw mixture, mixing the bacteria liquid mixture with the straw mixture, and drying to obtain the bacteria powder.
8. The method of claim 7, wherein the surfactant is glycerol.
9. The method for the microorganism-induced in-situ advanced treatment of the historical residual oily sludge in the oilfield mining area according to claim 1, wherein in S5, the plant is a mixed seed obtained by mixing the grass seeds of corn grass, ryegrass or green bristlegrass in a mass ratio of 1; the addition amount of the fungus powder is 0.2-0.5 times of the previous fungus powder.
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