CN113476360A - Composition for improving pouch and preparation method and application thereof - Google Patents
Composition for improving pouch and preparation method and application thereof Download PDFInfo
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- CN113476360A CN113476360A CN202110690534.1A CN202110690534A CN113476360A CN 113476360 A CN113476360 A CN 113476360A CN 202110690534 A CN202110690534 A CN 202110690534A CN 113476360 A CN113476360 A CN 113476360A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
Abstract
The invention discloses a composition for improving pouch, a preparation method and an application thereof, and belongs to the technical field of cosmetics. The composition for improving the eye bags comprises dihydroartemisinin and a lotus leaf extract, wherein the weight ratio of the dihydroartemisinin to the lotus leaf extract is 0.01-0.1: 1. the pouch-improving composition of the present invention can inhibit true pouch formation by three aspects: the eye cream is antioxidant, inhibits the damage of free radicals to eye skin and prevents the eye skin from aging and sagging; secondly, the degradation of collagen is inhibited, and the elasticity of eye skin is prevented from being reduced; thirdly, the differentiation of 3T3-L1 preadipocytes is inhibited, and the generation of fat is inhibited. Aiming at the reasons of pouch formation, the composition of the invention can improve pouch fundamentally.
Description
Technical Field
The invention relates to a composition for improving pouches and a preparation method and application thereof, belonging to the field of cosmetics.
Background
The eye bags are generally divided into true eye bags and edema type eye bags, the true eye bags are mainly characterized in that collagen and fat are loosened with age, so that tension between skin and an orbit is reduced, fat and tissues in the orbit are pushed out of an eye diaphragm through an orbit diaphragm weak link, and the eye diaphragm is expressed as loose skin of a lower eyelid of an eye part; edema type eye bags. The eye typically retains much of the fluid as a result of fluid remaining in the periocular tissues while lying flat or pressure (cortisol) or circulation problems, manifested as temporary edema, dark circles and puffy eyes. The real eye bags mainly appear in middle-aged and old people or young people with heredity at the age of forty and above, and the real eye bags often appear in people above 40 years are also found out of the young people along with the aggravation of life rhythm, severe decoction, air pollution, pressure and the like. In order to solve the true pouches which appear in young people, the invention develops a skin care composition based on the mechanism of the true pouches, and the skin care composition can improve the pouches from the pathways of inhibiting collagen degradation, resisting oxidation, digesting fat and the like.
Disclosure of Invention
The invention aims to provide a composition capable of improving pouches and a preparation method and application thereof.
In order to achieve the purpose, the invention adopts the technical scheme that:
a composition for improving eye bags comprises dihydroartemisinin and a lotus leaf extract, wherein the weight ratio of the dihydroartemisinin to the lotus leaf extract is 0.01-0.1: 1.
the folium Nelumbinis extract mainly contains alkaloids, flavonoids, volatile oils, etc., and has antibacterial and anti-inflammatory effects; dihydroartemisinin is a derivative of artemisinin, and most of artemisinin derivatives have the effects of inhibiting pigmentation, inhibiting scar formation and delaying skin aging. According to the invention, dihydroartemisinin and lotus leaf extract are combined, and the composition has a good synergistic effect on the aspect of improving eye bags. The composition of the present invention can inhibit true pouch formation by three aspects: the eye cream is antioxidant, inhibits the damage of free radicals to eye skin and prevents the eye skin from aging and sagging; secondly, the degradation of collagen is inhibited, and the elasticity of eye skin is prevented from being reduced; thirdly, the differentiation of 3T3-L1 preadipocytes is inhibited, and the generation of fat is inhibited. Aiming at the reasons of pouch formation, the composition of the invention can improve pouch fundamentally.
As a preferable embodiment of the composition for improving the eye bags, the weight ratio of the dihydroartemisinin to the lotus leaf extract is 0.03-0.06: 1. the weight ratio of the dihydroartemisinin to the lotus leaf extract has great influence on the pouch improvement effect of the prepared composition, and within the weight ratio range, the synergistic effect of the dihydroartemisinin and the lotus leaf extract is stronger, and the pouch improvement effect is better.
The invention also provides a preparation method of the composition for improving the pouch, which comprises the following steps:
(1) mixing folium Nelumbinis with methyl propylene glycol water solution, ultrasonic extracting, filtering to obtain filtrate to obtain folium Nelumbinis crude extract;
(2) filtering the crude extract of lotus leaves for the second time to obtain filtrate, thus obtaining a lotus leaf extract;
(3) mixing dihydroartemisinin with the lotus leaf extract, and dissolving with ultrasonic to obtain the composition for improving eye bags.
As a preferred embodiment of the method for preparing the composition for improving under-eye puffiness according to the present invention, the weight ratio of lotus leaves to the methyl propylene glycol aqueous solution in the step (1) is: 1: 1 to 15. Research shows that the weight ratio of lotus leaves to the methyl propylene glycol aqueous solution influences the extraction of the total flavone in the lotus leaves, the extraction amount of the total flavone is not in positive correlation with the using amount of the methyl propylene glycol aqueous solution, and when the using amount of the methyl propylene glycol aqueous solution is increased, the extraction amount of the lotus leaf brown brass is not increased.
As a preferred embodiment of the method for preparing the composition for improving pouches of the present invention, the weight ratio of the methyl propylene glycol to the water in the methyl propylene glycol aqueous solution in the step (1) is: 2-5: 1 of the total amount of the active ingredients,
as a preferred embodiment of the method for preparing the composition for improving pouches of the present invention, the weight ratio of the methyl propylene glycol to the water in the methyl propylene glycol aqueous solution in the step (1) is: 4:1. Research shows that the weight ratio of the methyl propylene glycol to the water in the methyl propylene glycol aqueous solution has great influence on the extraction of the total flavone in the lotus leaves, and the lotus leaf extract finally extracted has higher content of the total flavone by selecting the weight ratio.
As a preferred embodiment of the preparation method of the composition for improving the bags under the eyes, the ultrasonic power in the step (1) is 200-800W, the ultrasonic time is 30-80 min, and the ultrasonic temperature is 30-60 ℃;
the aperture of the filter membrane filtered in the step (1) is 0.1-0.3 um;
the aperture of the filter membrane for secondary filtration in the step (2) is 10-100 KDa;
the ultrasonic power in the step (3) is 300-600W, the ultrasonic time is 10-25 min, and the ultrasonic temperature is 10-30 ℃. Research shows that the selection of the ultrasonic condition has great influence on the extraction amount of the total flavonoids in lotus leaves and the improvement effect of the eye bags of the finally prepared composition, the extraction amount of the total flavonoids in lotus leaves is higher under the ultrasonic condition in the range, and the ultrasonic condition in the range is selected to fully mix the dihydroartemisinin and the lotus leaf extract, so that the dihydroartemisinin and the lotus leaf extract synergistically improve the effect of improving the eye bags.
The invention also provides application of the composition for improving the bags under the eyes in preparing a skin care product for improving the bags under the eyes.
As a preferred embodiment of the application of the pouch-improving composition in the invention in preparing a pouch-improving skin care product, the pouch-improving skin care product is one of eye cream, eye gel and eye mask.
The invention also provides an eye cream for improving the bags under the eyes, which comprises the composition for improving the bags under the eyes and a substrate; the composition comprises the following components of the composition, wherein the composition comprises the following components of the composition, and the composition comprises the following components of the composition, such as the glyceryl polyether, disodium ethylene diamine tetraacetate, carbomer, p-hydroxyacetophenone, cetostearyl alcohol, cetostearyl glucoside, polydimethylsiloxane, dimethiconol, hydrogenated polyisobutene, hydrogenated lecithin, palmitic acid, hydroxyethyl acrylate, sodium acryloyldimethyl taurate copolymer, squalane, polysorbate-60, sodium hydroxide and 1, 2-hexanediol.
Compared with the prior art, the invention has the beneficial effects that: the invention provides a composition for improving eye bags, which is prepared by compounding dihydroartemisinin and lotus leaf extract for the first time and inhibits true eye bag formation through three aspects: the eye cream is antioxidant, inhibits the damage of free radicals to eye skin and prevents the eye skin from aging and sagging; secondly, the degradation of collagen is inhibited, and the elasticity of eye skin is prevented from being reduced; thirdly, the differentiation of 3T3-L1 preadipocytes is inhibited, the generation of fat is inhibited, the preparation process of the composition for improving the pouch is simple, and the pouch can be effectively improved.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.
Example 1
The preparation method of the composition for improving the pouch of the embodiment of the invention comprises the following steps:
(1) weighing the components in a weight ratio of 1: 8, ultrasonically extracting lotus leaves and a methyl propylene glycol aqueous solution for 50min under the conditions of ultrasonic power of 600W and ultrasonic temperature of 45 ℃, and filtering by using a filter membrane with the aperture of 0.2 mu m to obtain filtrate to obtain a lotus leaf crude extract; the weight ratio of the methyl propylene glycol to the water in the methyl propylene glycol aqueous solution is 4: 1;
(2) secondarily filtering the lotus leaf crude extract obtained in the step (1) by a filter membrane with the aperture of 50KDa to obtain filtrate, thus obtaining a lotus leaf extract;
(3) mixing the components in a weight ratio of 0.05: 1, mixing the dihydroartemisinin and the lotus leaf extract, and ultrasonically dissolving for 15min under the conditions of the ultrasonic power of 400W and the ultrasonic temperature of 20 ℃ to obtain the composition for improving the eye bags.
Example 2
The preparation method of the composition for improving the pouch of the embodiment of the invention comprises the following steps:
(1) weighing the components in a weight ratio of 1: 10, ultrasonically extracting lotus leaves and a methyl propylene glycol aqueous solution for 60min under the conditions of ultrasonic power of 500W and ultrasonic temperature of 50 ℃, and filtering by a filter membrane with the aperture of 0.2 mu m to obtain filtrate to obtain a lotus leaf crude extract; the weight ratio of the methyl propylene glycol to the water in the methyl propylene glycol aqueous solution is 4: 1;
(2) secondarily filtering the crude lotus leaf extract obtained in the step (1) by using a filter membrane with the aperture of 60KDa to obtain filtrate, thus obtaining a lotus leaf extract;
(3) mixing the components in a weight ratio of 0.04: 1, mixing the dihydroartemisinin and the lotus leaf extract, and performing ultrasonic dissolution for 12min under the conditions of ultrasonic power of 500W and ultrasonic temperature of 15 ℃ to obtain the composition for improving the eye bags.
Example 3
The preparation method of the composition for improving the pouch of the embodiment of the invention comprises the following steps:
(1) weighing the components in a weight ratio of 1: 5, ultrasonically extracting the lotus leaves and the methyl propylene glycol aqueous solution for 30min under the conditions of ultrasonic power of 200W and ultrasonic temperature of 60 ℃, and filtering by using a filter membrane with the aperture of 0.1 mu m to obtain filtrate to obtain a lotus leaf crude extract; the weight ratio of the methyl propylene glycol to the water in the methyl propylene glycol aqueous solution is 2: 1;
(2) secondarily filtering the lotus leaf crude extract obtained in the step (1) by a filter membrane with the aperture of 10KDa to obtain filtrate, thus obtaining a lotus leaf extract;
(3) mixing the components in a weight ratio of 0.01: 1, mixing the dihydroartemisinin and the lotus leaf extract, and ultrasonically dissolving for 10min under the conditions of the ultrasonic power of 300W and the ultrasonic temperature of 10 ℃ to obtain the composition for improving the eye bags.
Example 4
The preparation method of the composition for improving the pouch of the embodiment of the invention comprises the following steps:
(1) weighing the components in a weight ratio of 1: 15, ultrasonically extracting lotus leaves and a methyl propylene glycol aqueous solution for 80min under the conditions of the ultrasonic power of 800W and the ultrasonic temperature of 30 ℃, and filtering by using a filter membrane with the aperture of 0.3 mu m to obtain a filtrate to obtain a lotus leaf crude extract; the weight ratio of the methyl propylene glycol to the water in the methyl propylene glycol aqueous solution is 5: 1;
(2) secondarily filtering the lotus leaf crude extract obtained in the step (1) by a filter membrane with the aperture of 100KDa to obtain filtrate, thus obtaining a lotus leaf extract;
(3) mixing the components in a weight ratio of 0.1: 1, mixing the dihydroartemisinin and the lotus leaf extract, and ultrasonically dissolving for 25min under the conditions of the ultrasonic power of 600W and the ultrasonic temperature of 30 ℃ to obtain the composition for improving the eye bags.
Example 5
The preparation method of the composition for improving the pouch of the embodiment of the invention comprises the following steps:
(1) weighing the components in a weight ratio of 1: 8, ultrasonically extracting lotus leaves and a methyl propylene glycol aqueous solution for 80min under the conditions of ultrasonic power of 200W and ultrasonic temperature of 30 ℃, and filtering by using a filter membrane with the aperture of 0.3 mu m to obtain filtrate to obtain a lotus leaf crude extract; the weight ratio of the methyl propylene glycol to the water in the methyl propylene glycol aqueous solution is 4: 1;
(2) secondarily filtering the lotus leaf crude extract obtained in the step (1) by a filter membrane with the aperture of 100KDa to obtain filtrate, thus obtaining a lotus leaf extract;
(3) mixing the components in a weight ratio of 0.05: 1, mixing the dihydroartemisinin and the lotus leaf extract, and ultrasonically dissolving for 15min under the conditions of the ultrasonic power of 400W and the ultrasonic temperature of 20 ℃ to obtain the composition for improving the eye bags.
Example 6
The preparation method of the composition for improving the pouch of the embodiment of the invention comprises the following steps:
(1) weighing the components in a weight ratio of 1: 8, ultrasonically extracting lotus leaves and a methyl propylene glycol aqueous solution for 50min under the conditions of ultrasonic power of 600W and ultrasonic temperature of 45 ℃, and filtering by using a filter membrane with the aperture of 0.2 mu m to obtain filtrate to obtain a lotus leaf crude extract; the weight ratio of the methyl propylene glycol to the water in the methyl propylene glycol aqueous solution is 4: 1;
(2) secondarily filtering the lotus leaf crude extract obtained in the step (1) by a filter membrane with the aperture of 50KDa to obtain filtrate, thus obtaining a lotus leaf extract;
(3) mixing the components in a weight ratio of 0.05: 1, mixing the dihydroartemisinin and the lotus leaf extract, and ultrasonically dissolving for 25min under the conditions of the ultrasonic power of 300W and the ultrasonic temperature of 30 ℃ to obtain the composition for improving the eye bags.
Example 7
The preparation method of the composition for improving the pouch of the embodiment of the invention comprises the following steps:
(1) weighing the components in a weight ratio of 1: 8, ultrasonically extracting lotus leaves and a methyl propylene glycol aqueous solution for 50min under the conditions of ultrasonic power of 600W and ultrasonic temperature of 45 ℃, and filtering by using a filter membrane with the aperture of 0.2 mu m to obtain filtrate to obtain a lotus leaf crude extract; the weight ratio of the methyl propylene glycol to the water in the methyl propylene glycol aqueous solution is 4: 1;
(2) secondarily filtering the lotus leaf crude extract obtained in the step (1) by a filter membrane with the aperture of 50KDa to obtain filtrate, thus obtaining a lotus leaf extract;
(3) mixing the components in a weight ratio of 0.05: 1, mixing the dihydroartemisinin and the lotus leaf extract, and performing ultrasonic dissolution for 15min under the conditions of ultrasonic power of 600W and ultrasonic temperature of 100 ℃ to obtain the composition for improving the eye bags.
Comparative example 1
The comparative example differs from example 1 only in that the weight ratio of methyl propylene glycol to water in the aqueous methyl propylene glycol solution is 4: 0.
comparative example 2
The present comparative example differs from example 1 only in that the weight ratio of methyl propylene glycol to water in the aqueous methyl propylene glycol solution is 0: 1.
comparative example 3
The comparative example differs from example 1 only in that the weight ratio of lotus leaves to the aqueous solution of methyl propylene glycol is 1: 16.
Comparative example 4
The comparative example differs from example 1 only in that the weight ratio of lotus leaves to the aqueous solution of methyl propylene glycol is 0.8: 8.
comparative example 5
The comparative example is different from example 1 only in that the ultrasonic extraction conditions in step (1) are 100W of ultrasonic power, 70 ℃ of ultrasonic temperature and 70min of ultrasonic time.
Comparative example 6
The comparative example is different from example 1 only in that the ultrasonic extraction conditions in step (1) are ultrasonic power of 900W, ultrasonic temperature of 25 ℃ and ultrasonic time of 25 min.
Comparative example 7
This comparative example differs from example 1 only in that the filter pore size of the filtration in step (1) was 0.05. mu.m, and the filter pore size of the secondary filtration in step (2) was 5 kDa.
Comparative example 8
This comparative example differs from example 1 only in that the filter pore size of the filtration in step (1) was 0.4. mu.m, and the filter pore size of the secondary filtration in step (2) was 150 kDa.
Comparative example 9
The present comparative example differs from example 1 only in that the weight ratio of dihydroartemisinin to the lotus leaf extract in step (3) is 0.005: 1.
comparative example 10
The present comparative example differs from example 1 only in that the weight ratio of dihydroartemisinin to the lotus leaf extract in step (3) is 0.15: 1.
comparative example 11
The comparative example is different from example 1 only in that the ultrasonic dissolution conditions in step (3) are ultrasonic power of 200W, ultrasonic temperature of 35 ℃ and ultrasonic time of 30 min.
Comparative example 12
The comparative example is different from example 1 only in that the ultrasonic dissolution conditions in step (3) are ultrasonic power of 700W, ultrasonic temperature of 5 ℃ and ultrasonic time of 8 min.
Comparative example 13
(1) Weighing the components in a weight ratio of 1: 8, ultrasonically extracting lotus leaves and a methyl propylene glycol aqueous solution for 50min under the conditions of ultrasonic power of 600W and ultrasonic temperature of 45 ℃, and filtering by using a filter membrane with the aperture of 0.2 mu m to obtain filtrate to obtain a lotus leaf crude extract; the weight ratio of the methyl propylene glycol to the water in the methyl propylene glycol aqueous solution is 4: 1;
(2) secondarily filtering the lotus leaf crude extract obtained in the step (1) by a filter membrane with the aperture of 50KDa to obtain filtrate, thus obtaining a lotus leaf extract;
(3) weighing 1 part by weight of lotus leaf extract for later use.
Comparative example 14
Mixing the components in a weight ratio of 0.05: 1 and mixing the dihydroartemisinin with deionized water, and ultrasonically dissolving for 15min under the conditions of the ultrasonic power of 400W and the ultrasonic temperature of 20 ℃ to obtain a dihydroartemisinin solution.
Test examples
Eye creams are prepared by using the compositions for improving bags in examples 1-7 and comparative examples 1-12 and the solutions in comparative examples 13-14 respectively, and the steps are as follows:
(1) 79.215 parts of deionized water, 263.0 parts of glyceryl polyether, 0.15 part of EDTA-2Na, 0.1 part of carbomer and 0.6 part of p-hydroxyacetophenone are weighed, mixed uniformly and heated to 85 ℃ to obtain phase A;
(2) weighing 0.1 part of MONTANOV 68MB (cetearyl alcohol, cetearyl glucoside), 5.0 parts of polydimethylsiloxane, 2.0 parts of PMX-1503 (polydimethylsiloxane), 3.0 parts of hydrogenated polyisobutene, 0.2 part of hydrogenated lecithin, 0.2 part of palmitic acid and 1.8 parts of SIMULGEC NS (hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, squalane and polysorbate-60), uniformly mixing and heating to 85 ℃ to obtain a phase B;
(3) weighing 2 parts of deionized water and 0.035 part of NaOH, mixing uniformly, and adding to 85 ℃ to obtain a phase C;
(4) weighing 2.0 parts of the composition for improving the pouch, heating to 45 ℃, and stirring to obtain a phase D;
(5) adding phase B into phase A, homogenizing at 1200r/min for 6min, completely homogenizing, and adding phase C to obtain matrix; and (3) cooling the matrix to 45 ℃ while stirring, sequentially adding 0.6 part of 1, 2-hexanediol and the phase D, and uniformly mixing to obtain the eye cream for improving the pouches.
Examples of effects
1. Method for extracting total flavone
Using NaNO2-Al(NO3)3The total flavone content in the lotus leaf extracts of examples 1 to 7 and comparative examples 1 to 8 was measured by NaOH colorimetry, and the results are shown in Table 1.
TABLE 1
| The content of total flavone is mg/ml | Appearance of the product | |
| Example 1 | 1.02 | Light yellow clear and bright liquid |
| Example 2 | 0.95 | Light yellow clear and bright liquid |
| Example 3 | 0.62 | Light yellow clear and bright liquid |
| Example 4 | 0.65 | Light yellow clear and bright liquid |
| Example 5 | 0.75 | Light yellow clear and bright liquid |
| Example 6 | 0.79 | Light yellow clear and bright liquid |
| Example 7 | 0.85 | Light yellow clear and bright liquid |
| Comparative example 1 | 0.48 | Light yellow clear and bright liquid |
| Comparative example 2 | 0.32 | Light yellow clear and bright liquid |
| Comparative example 3 | 0.53 | Light yellow clear and bright liquid |
| Comparative example 4 | 0.54 | Yellow clear and bright liquid |
| Comparative example 5 | 0.56 | Light yellow clear and bright liquid |
| Comparative example 6 | 0.54 | Light yellow clear and bright liquid |
| Comparative example 7 | 0.51 | Light yellow clear and bright liquid |
| Comparative example 8 | 1.25 | Yellow atomized liquid |
As can be seen from Table 1, the comparison of the data of examples 1-7 and comparative examples 1-8 shows that the products obtained in the examples are better than the comparative examples in terms of total flavone content or appearance. The high content of the total flavonoids indicates that the obtained composition has good oxidation resistance, although the content of the total flavonoids is high in the comparative example 8, the main reason is that the aperture of a membrane used for filtering is large, a plurality of macromolecular substances are not removed, and the numerical value is large because the macromolecular substances contain flavonoids, but the macromolecular substances are not removed, so that the product has an unclear and transparent appearance, poor stability and is easy to precipitate and discolor, the influence on the stability of an eye cream product is large, and the phenomena of precipitation and discoloration are easy to generate.
2. Inhibition of 3T3-L1 preadipocytes
(1) Test method
Detecting by adopting an MTT method: cells were seeded at a density of 1 × 105/mL onto 96-well plates, cultured for 24h until confluency of growth, and divided into normal control groups and sample groups with a mass fraction of 1 g/mL. Normal control group cells are cultured normally; the sample groups stimulate 3T3-L1 precursor adipocytes for 24h by using examples 1-7 and comparative examples 1-14 respectively, then replace 90 mu L of serum-free medium containing 5g/L of MTT solution, continue to culture for 4h, and then measure the absorbance at 490nm by using a microplate reader. The inhibition rate was calculated according to the following formula
Wherein A1 is the absorbance value of the normal control group and A2 is the absorbance value of the sample group.
(2) Results of the experiment
The specific inhibition rates are shown in Table 2.
TABLE 2
| Sample (I) | Inhibition rate |
| Example 1 | 23.0％ |
| Example 2 | 21.0％ |
| Example 3 | 16.0％ |
| Example 4 | 17.5％ |
| Example 5 | 19.8％ |
| Example 6 | 20.0％ |
| Example 7 | 20.0％ |
| Comparative example 1 | 5.0％ |
| Comparative example 2 | 3.0％ |
| Comparative example 3 | 11.12％ |
| Comparative example 4 | 12.45％ |
| Comparative example 5 | 13.5％ |
| Comparative example 6 | 13.1％ |
| Comparative example 7 | 11.04％ |
| Comparative example 8 | 14.0％ |
| Comparative example 9 | 11.75％ |
| Comparative example 10 | 12.34％ |
| Comparative example 11 | 11.24％ |
| Comparative example 12 | 10.45％ |
| Comparative example 13 | 5.0％ |
| Comparative example 14 | 6.50％ |
As can be seen from table 2, the pouch-improving composition of the present invention has an effect of inhibiting the regeneration of adipocytes, and can improve pouch problems caused by accumulation of fat; example 1 shows that the inhibition rate of 3T3-L1 preadipocytes is far greater than that of comparative examples 13 and 14, and the inhibition effect of dihydroartemisinin and lotus leaf extract on adipocyte regeneration is enhanced through the synergistic effect.
3. Pouch improvement test
(1) Test method
210 women with real pouches on the face between 30 and 65 years old are selected as trial objects and divided into 21 groups of 10 persons, and one sample is tried on each group. Before the test, after cleaning face, the tester is in constant temperature and humidity environment (temperature 21-23 deg.C, humidity 55-65%) for 30min, and then takes facial photograph by VISA-CR as primary eye pouch data, the subject applies each sample to eye periphery, uses each sample for 1 time every day, continuously tries for 56 days, cleans face on 30 th and 56 th days, then takes eye pouch test by VISA-CR, and records as D30And D56. It is noted that each group D30And D56The values are the average of the set of values and the pouch improvement before and after use is calculated by the instrument software.
(2) Test results
The specific pouch-improving effect is shown in table 3.
TABLE 3
| D30 | D56 | |
| Example 1 | 6％ | 18.34％ |
| Example 2 | 5％ | 17.25％ |
| Example 3 | 4％ | 16.25％ |
| Example 4 | 4％ | 15.78％ |
| Example 5 | 4％ | 14.56％ |
| Example 6 | 4％ | 15.01％ |
| Example 7 | 4％ | 15.03％ |
| Comparative example 1 | 4％ | 8.52％ |
| Comparative example 2 | 4％ | 7.12％ |
| Comparative example 3 | 3％ | 6.51％ |
| Comparative example 4 | 3％ | 5.68％ |
| Comparative example 5 | 2％ | 4.21％ |
| Comparative example 6 | 2％ | 3.65％ |
| Comparative example 7 | 1％ | 3.56％ |
| Comparative example 8 | 1％ | 2.56％ |
| Comparative example 9 | 1％ | 2.78％ |
| Comparative example 10 | 1％ | 3.78％ |
| Comparative example 11 | 1％ | 3.04％ |
| Comparative example 12 | 1％ | 2.78％ |
| Comparative example 13 | 0％ | 2.14％ |
| Comparative example 14 | 0％ | 1.78％ |
As can be seen from table 3, the eye creams of examples 1 to 7 have a significant pouch-improving effect; example 1 is more effective in improving pouch than the eye creams of comparative examples 13 and 14, which shows that the pouch-improving composition of the present invention has a better pouch-improving effect through the synergistic effect of dihydroartemisinin and lotus leaf extract.
Although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the true spirit and scope of the present invention.
Claims (10)
1. A composition for improving eye bags, which comprises dihydroartemisinin and lotus leaf extract; the weight ratio of the dihydroartemisinin to the lotus leaf extract is 0.01-0.1: 1.
2. the pouch-improving composition according to claim 1, wherein the weight ratio of dihydroartemisinin to lotus leaf extract is 0.03-0.06: 1.
3. the method for preparing a pouch-improving composition according to any one of claims 1 to 2, comprising the steps of:
(1) mixing folium Nelumbinis with methyl propylene glycol water solution, ultrasonic extracting, filtering to obtain filtrate to obtain folium Nelumbinis crude extract;
(2) filtering the crude extract of lotus leaves for the second time to obtain filtrate, thus obtaining a lotus leaf extract;
(3) mixing dihydroartemisinin with the lotus leaf extract, and dissolving with ultrasonic to obtain the composition for improving eye bags.
4. The method for preparing a composition for improving under-eye puffiness as claimed in claim 3, wherein the weight ratio of lotus leaves to the aqueous solution of methyl propylene glycol in step (1) is: 1: 1 to 15.
5. The method for preparing a composition for improving under-eye puffiness as claimed in claim 3, wherein the weight ratio of the methyl propylene glycol to the water in the methyl propylene glycol aqueous solution in the step (1) is: 2-5: 1.
6. The method for preparing a composition for improving under-eye puffiness as claimed in claim 5, wherein the weight ratio of the methyl propylene glycol to the water in the aqueous methyl propylene glycol solution in step (1) is: 4:1.
7. The method for preparing the composition for improving the bags under the eyes according to claim 3, wherein the ultrasonic power in the step (1) is 200-800W, the ultrasonic time is 30-80 min, and the ultrasonic temperature is 30-60 ℃;
the aperture of the filter membrane filtered in the step (1) is 0.1-0.3 μm;
the aperture of the filter membrane for secondary filtration in the step (2) is 10-100 KDa;
the ultrasonic power in the step (3) is 300-600W, the ultrasonic time is 10-25 min, and the ultrasonic temperature is 10-30 ℃.
8. Use of the pouch-improving composition according to any one of claims 1 to 2 for preparing a pouch-improving skin care product.
9. The use of claim 8, wherein the pouch-improving skin care product is one of eye cream, eye gel and eye mask.
10. An eye cream for improving pouches, comprising the pouch-improving composition according to any one of claims 1 to 2 and a base; the matrix comprises glyceryl polyether, ethylene diamine tetraacetic acid disodium, carbomer, p-hydroxyacetophenone, cetostearyl alcohol, cetearyl glucoside, polydimethylsiloxane, dimethiconol, hydrogenated polyisobutene, hydrogenated lecithin, palmitic acid, hydroxyethyl acrylate, sodium acryloyldimethyl taurate copolymer, squalane, polysorbate-60, sodium hydroxide and 1, 2-hexanediol.
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