CN113439658A - 一种高效诱导蝴蝶兰双梗的方法 - Google Patents

一种高效诱导蝴蝶兰双梗的方法 Download PDF

Info

Publication number
CN113439658A
CN113439658A CN202110720751.0A CN202110720751A CN113439658A CN 113439658 A CN113439658 A CN 113439658A CN 202110720751 A CN202110720751 A CN 202110720751A CN 113439658 A CN113439658 A CN 113439658A
Authority
CN
China
Prior art keywords
phalaenopsis
stems
double
plants
flower
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN202110720751.0A
Other languages
English (en)
Other versions
CN113439658B (zh
Inventor
张海波
吴笛
吴锦华
李林宝
张定军
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
China Three Gorges Corp
Original Assignee
China Three Gorges Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by China Three Gorges Corp filed Critical China Three Gorges Corp
Priority to CN202110720751.0A priority Critical patent/CN113439658B/zh
Publication of CN113439658A publication Critical patent/CN113439658A/zh
Priority to JP2023508010A priority patent/JP2023537367A/ja
Priority to DE112022000057.0T priority patent/DE112022000057B4/de
Priority to PCT/CN2022/082907 priority patent/WO2022252777A1/zh
Priority to GB2216951.0A priority patent/GB2613254B/en
Application granted granted Critical
Publication of CN113439658B publication Critical patent/CN113439658B/zh
Priority to DKPA202270523A priority patent/DK181430B1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
    • A01H3/04—Processes for modifying phenotypes, e.g. symbiosis with bacteria by treatment with chemicals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/02—Flowers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/04—Stems
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/62—Orchidaceae [Orchid family]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/647—Triazoles; Hydrogenated triazoles
    • A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/26—Phosphorus; Compounds thereof

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • General Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Dentistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Cultivation Of Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

本发明属于标本制备技术领域，具体涉及一种高效诱导蝴蝶兰双梗的方法，蝴蝶兰在经过化学药剂处理组合(TDZ 0.01～0.05g/L+GA3 0.3～1.0g/L+IAA0.1～0.6g/L+PP3330.2～0.8g/L+P2O50.5～1.2g/L+谷氨酸0.8～1.5g/L)处理后，能快速高效的诱导出双梗。此方法实施起来简单且省时省力，为蝴蝶兰提高产品质量，增强蝴蝶兰的观赏价值提供了一条快速有效的途径。本发明方法适合蝴蝶兰的工厂化催花,且生产成本低,得到的蝴蝶兰品质高，一致性好，具有低投入，高收益的优点，表现出了较好地推广应用价值。

Description

一种高效诱导蝴蝶兰双梗的方法
技术领域
本发明涉及标本制备技术领域，具体涉及一种高效诱导蝴蝶兰双梗的方法。
背景技术
蝴蝶兰(Phalaenopsis spp.)为兰科蝴蝶兰属花卉。因其花型奇特，色彩艳丽，花期持久，素有“洋兰皇后”之美誉。
刘耀仁刊载的“双层充气式蝴蝶兰催花温室抽梗率及双梗率之调查”一文中不同蝴蝶兰品种在经过30余天催花后双梗诱导率依次为：夕阳红50％、劳伦斯27％、V317％、巨宝红玫瑰仅为3％。在《中国花卉报》今年3月底刊发的文章《年宵花中的王者，凭什么是蝴蝶兰》写到，2020年宵期间，我国蝴蝶兰上市量预估超过5500万株，预计总销售额超过10亿元，是排在后面其他几个主要品种的销售额总和。在蝴蝶兰双梗品种方面，欧洲相比中国极具优势，欧洲的育种者已经培育了20多年双梗品种。就中小花型而言，对种植者来说，双梗品种的经济效益比单梗品种高。对于中国的经销商客户，如果单梗蝴蝶兰产品进货价为20元，优质双梗产品进货价则一般为25元，最高溢价达25％。由此可见做好蝴蝶兰双梗研究对于提高人民生活质量，建设美丽中国具有重要现实意义。
发明内容
为解决上述技术问题，本发明提供一种高效诱导蝴蝶兰双梗的方法，达到了培育双梗品种的观赏效果，但却大大节省了育种所需要的时间成本。
本发明技术方案如下：
一种高效诱导蝴蝶兰双梗的方法，所述方法包括以下步骤：
步骤一、植株物理处理：将预进行催花处理的蝴蝶兰植株统一进行物理平面调整和空间角度调整；
步骤二、化学试剂处理：完成步骤一后，对蝴蝶兰植株叶片2～5片的茎段喷施花梗诱导混合溶剂液，花梗诱导混合溶剂处理每10d一次，共计喷施3次；完成蝴蝶兰双梗的高效诱导。
优选地，所述步骤一中，物理平面调整具体操作为：将所有蝴蝶兰植株统一朝向，植株茎和叶片生长方向朝北，根部朝南。
优选地，所述步骤一中，空间角度调整具体操作为：即将所有催花前蝴蝶兰植株统一抬高倾斜角，使植株与摇床底面呈70～90度角，光线能够由上而下照射到蝴蝶兰植株叶片3～5片的茎段叶腋处，待催花30～40d花梗长至10～20cm后去掉倾斜角恢复0度或180度摆放。
优选地，所述步骤二中，花梗诱导混合溶剂液为：TDZ 0.01～0.05g/L+GA3 0.3～1.0g/L+IAA0.1～0.6g/L+PP3330.2～0.8g/L+P2O50.5～1.2g/L+谷氨酸0.8～1.5g/L。
优选地，所述步骤二中，喷施的方法为每次喷施时采用150ml小喷壶，壶口调成出雾状最佳状态围绕准蝴蝶兰植株叶片2～5片的茎段一周喷施，着重喷施植株叶腋处，喷完后水雾附着在茎段表面不流动为宜。
优选地，所述步骤二中，花梗诱导混合喷施处理每9-12d一次，共计喷施2-4次。
本发明有益效果为：
1、室外植株处理操作简单，效果显著，定期内可以诱导出蝴蝶兰双梗。
2、蝴蝶兰植株物理平面调整不但可以使磁场平稳地穿过植株本体，将地球磁场对植株的影响降到最低外，便于对植株进行施肥、浇水和换盆等操作。
3、蝴蝶兰植株物理空间角度调整更有利于光线能够由上而下照射到蝴蝶兰植株叶片3～5片的茎段叶腋处，促进双梗萌发。
4、化学药剂处理能有效促进植株由营养生长转向生殖生长，促进花芽分化和双梗率，从而在大规模生产上有着重要意义。
附图说明
图1、蝴蝶兰叶片平面一致性朝向；
图2、蝴蝶兰植株空间倾斜角度处理；
图3、蝴蝶兰群株双梗诱导；
图4、蝴蝶兰单株双梗诱导；
图5、蝴蝶兰红色系(红太阳)双梗开花株；
图6、蝴蝶兰黄色系(富乐夕阳)双梗群株开花；
图7、蝴蝶兰橙色系(金蝶)双梗群株开花；
图8、蝴蝶兰红色系(世纪红)双梗群株开花。
具体实施方式
为了使本发明的目的、技术方案及优点更加清楚明白，以下结合实施例，对本发明进行进一步详细说明。应当理解，此处所描述的具体实施例仅仅用以解释本发明，并不用于限定本发明。
实施例1
1、植株物理处理
为顺利有效促进双梗的萌发，先对蝴蝶兰植株进行物理处理，主要是将预进行催花处理的蝴蝶兰植株统一进行物理平面和空间角度调整。一是进行物理平面调整，即将所有蝴蝶兰植株统一朝向，本实验温室为南北走向(其它类似温室可根据实际情况进行调整)，植株茎和叶片生长方向朝北，根部朝南，保证植株顺着地球磁场的方向，这样除有利磁场平稳地穿过植株本体，将地球磁场对植株的影响降到最低外，还便于对植株进行施肥、浇水和换盆等操作(见图1)。二是在完成统一朝向调整后对植株进行空间角度调整，即将所有催花前蝴蝶兰植株统一抬高倾斜角，使植株与摇床底面呈70～90度角，光线能够由上而下照射到蝴蝶兰植株叶片3～5片的茎段叶腋处，待催花培养30～40d花梗长至10～20cm后去掉倾斜角恢复0度或180度摆放(见图2)。详细实验结果见表1至表3。
30d后统计，进行统一朝向处理的蝴蝶兰植株双梗诱导结果显示：只进行统一朝向处理的P1蝴蝶兰大花品种没有出现双梗现象，即双梗诱导率为0％；进行统一朝向处理的P2蝴蝶兰绒花品双梗诱导个数为5个，双梗诱导率5.6％；对照P3蝴蝶兰大花品种也为出现双梗，双梗诱导率为0％；对照P4蝴蝶兰绒花品双梗诱导个数为1个，双梗诱导率1.1％(见表1)。
30d后统计，进行空间角度处理的蝴蝶兰植株双梗诱导结果显示：单一进行空间70～90度角度处理的P5蝴蝶兰大花品种双梗诱导数为4个，双梗诱导率4.4％；进行空间70～90度角度处理的P6蝴蝶兰绒花品种双梗诱导数为9个，双梗诱导率10.0％；P5相比对照P3蝴蝶兰大花品种多出现4个双梗，双梗诱导率提高4.4％；P6比较对照P4蝴蝶兰绒花品双梗诱导个数多8个，双梗诱导率提升8.9％(见表2)。
30d后统计，进行统一朝向+处理空间角度处理的蝴蝶兰植株双梗诱导结果显示：进行统一朝向+空间70～90度角度处理的P7蝴蝶兰大花品种双梗诱导个数为71个，双梗诱导率78.9％；进行统一朝向+空间70～90度角度处理的P8蝴蝶兰绒花品种双梗诱导个数为82个，双梗诱导率91.1％；P7较对照P3蝴蝶兰大花品种双梗多出71个，双梗诱导率提高78.9％；P8较对照P4蝴蝶兰绒花品双梗诱导个数多出81个，双梗诱导率提高90％(见表3)。
表1蝴蝶兰催花培养30d双梗诱导情况
Figure BDA0003136428170000041
表2蝴蝶兰催花培养30d双梗诱导情况
Figure BDA0003136428170000042
表3蝴蝶兰催花培养30d双梗诱导情况
Figure BDA0003136428170000043
由表3可知：进行统一朝向+处理空间角度处理的蝴蝶兰植株无论是大花品种还是绒花品种，在蝴蝶兰双梗诱导个数和诱导率方面都有所提升，P8蝴蝶兰绒花品种较对照P3大花品种双梗诱导个数增加82个，诱导率提高91.1％；较P4绒花品种双梗诱导个数增加81个，诱导率提高90％；较P7大花品种双梗诱导个数增加11个，诱导率提高15.4％。P7大花品种较对照P3大花品种双梗诱导个数增加71个，诱导率提高78.9％；较P4绒花品种双梗诱导个数增加70个，诱导率提高77.8％。由此可见，统一朝向+处理空间角度处理对蝴蝶兰诱导双梗起着重要的作用。
2、植株化学试剂处理
植株化学药剂处理的研究具体为在完成统一朝向调整后及空间角度调整后，对蝴蝶兰植株叶片2～5片的茎段喷施花梗诱导混合溶剂液(TDZ 0.01～0.05g/L+GA3 0.3～1.0g/L+IAA0.1～0.6g/L+PP3330.2～0.8g/L+P2O50.5～1.2g/L+谷氨酸0.8～1.5g/L)，同时设立对照(不喷花梗诱导混合溶剂)。每个处理包含30盆植株个体，试验设重复3次。花梗诱导混合溶剂处理每10d一次，共计喷施3次。喷施的方法为每次喷施时采用150ml小喷壶，壶口调成出雾状最佳状态围绕准蝴蝶兰植株叶片2～5片的茎段一周喷施，着重喷施植株叶腋处，喷完后水雾附着在茎段表面不流动为宜。试验结果表明喷施花梗诱导混合溶剂的植株诱导率绒花品种为98.9％，大花品种为88.9％，绒花品种较大花品种双梗高出10％，绒花品种和大花品种平均双梗率为93.9％，而未喷施花梗诱导混合溶剂液的双梗率较低。详细实验结果见表4。
其中：
P9-P10：花梗诱导混合溶剂液A：TDZ 0.03g/L+GA3 0.6g/L+IAA0.4g/L+P2O50.5～1.2g/L+谷氨酸1.2g/L；
P11-P12：花梗诱导混合溶剂液B：TDZ 0.03g/L+GA30.6g/L+IAA0.4g/L+PP3330.5g/L+P2O50.9g/L；
P13-P14：花梗诱导混合溶剂液C：TDZ 0.03g/L+GA30.6g/L+IAA0.4g/L+PP3330.5g/L+P2O50.9g/L+谷氨酸1.2g/L；
P15-P16：花梗诱导混合溶剂液D：TDZ 0.03g/L+GA3 0.6g/L+IAA0.4g/L+P2O50.9g/L。
表4不同蝴蝶兰植株喷施花梗诱导混合溶剂对照表
Figure BDA0003136428170000051
Figure BDA0003136428170000061
30d后统计，在完成统一朝向调整及空间倾斜角度调整后，对蝴蝶兰植株喷施花梗诱导混合溶剂液A的结果显示：P9蝴蝶兰大花品种诱导双梗数量为72个，诱导率达80％，花梗长度为10.9cm，花梗粗度为0.41cm；P10蝴蝶兰绒花品种诱导个数为81个，诱导率90％，花梗长度为8.6cm，花梗粗度为0.31cm；
30d后统计，在完成统一朝向调整及空间倾斜角度调整后，对蝴蝶兰植株喷施花梗诱导混合溶剂液B的结果显示：P11蝴蝶兰大花品种诱导双梗数量为73个，诱导率达81.1％，花梗长度为9.2cm，花梗粗度为0.45cm；P12蝴蝶兰绒花品种诱导个数为82个，诱导率91.1％，花梗长度为7.7cm，花梗粗度为0.35cm；
30d后统计，在完成统一朝向调整及空间倾斜角度调整后，对蝴蝶兰植株喷施花梗诱导混合溶剂液C的结果显示：P14蝴蝶兰绒花品种诱导双梗数量为89个，诱导率达98.9％，花梗长度为10.5cm，花梗粗度为0.38cm(见图3、图6、图7)；P13蝴蝶兰大花品种次之，诱导个数为80个，诱导率88.9％，花梗长度为12.8cm，花梗粗度为0.52cm(见图4、图5、图8)；
30d后统计，在完成统一朝向调整及空间倾斜角度调整后，对蝴蝶兰植株喷施花梗诱导混合溶剂液D的结果显示：P15蝴蝶兰大花品种诱导双梗数量为70个，诱导率达77.8％，花梗长度为7.9cm，花梗粗度为0.39cm；P16蝴蝶兰绒花品种诱导个数为80个，诱导率88.9％，花梗长度为6.6cm，花梗粗度为0.25cm；
由表4可知：在完成统一朝向调整及空间倾斜角度调整后，对蝴蝶兰植株喷施花梗诱导混合溶剂液(TDZ 0.03g/L+GA3 0.6g/L+IAA0.4g/L+PP3330.5g/L+P2O50.9g/L+谷氨酸1.2g/L)，无论是在双梗诱导数量还是在提升诱导率方面效果都是最佳的。其中，P14蝴蝶兰绒花品种诱导效果最好，诱导数量最多为89个，诱导率最高达98.9％，花梗长度为10.5cm，花梗粗度为0.38cm(见图3、图6、图7)；P13蝴蝶兰大花品种次之，诱导个数为80个，诱导率88.9％，花梗长度为12.8cm，花梗粗度为0.52cm(见图4、图5、图8)；P14蝴蝶兰绒花品种较P3、P4、P9、P10、P11、P12、P13、P15和P16双梗数量分别增加89个、88个增长8800％、17个增长23.6％、8个增长9.9％、16个增长21.9％、7个增长8.5％、9个增长11.3％、19个增长24.1％和9个增长11.3％，诱导率分别提升98.9％、97.8％、18.9％、8.9％、17.8％、7.8％、10.0％、21.1％和10.0％，花梗长度分别增长10.5cm、5cm增幅91％、-0.4cm降幅3.7％、1.9cm增幅22.1％、1.3cm增幅14.1％、2.8cm增幅36.4％、-2.3cm降幅18.0％、2.6cm增幅32.9％和3.9cm增幅59.1％，花梗粗度分别增加0.38cm、0.15cm增幅65.2％、-0.03cm降幅7.3％、0.08cm增幅26.7％、-0.07cm减幅15.6％、0.03cm增幅8.6％、-0.14cm减幅26.9％、-0.01cm减幅2.6％和0.13cm增幅52％,绒花品种在花梗长度和花梗粗度方面有部分略低于大花品种属于品种本身特性决定，在关键性指标双梗个数和诱导率方面绒花品种占绝对优势；P13蝴蝶兰大花品种较P3、P4、P9、P10、P11、P12、P14、P15和P16双梗数量分别增加80个、79个增长7900％、8个增长11.1％、-1个减少1.2％、7个增长9.6％、-2个减少2.4％、-9个减少10.1％、10个增长14.3％和0个增长0.0％，诱导率分别提升88.9％、87.8％、8.9％、-1.1％、7.8％、-2.2％、-10.0％、11.1％和0.0％，花梗长度分别增长12.8cm、7.3cm增幅132.7％、1.9cm增幅17.4％、4.2cm增幅48.8％、3.6cm增幅39.1％、5.1cm增幅66.2％、2.3cm降幅21.9％、4.9cm增幅62.0％和6.2cm增幅93.9％，花梗粗度分别增加0.52cm、0.29cm涨幅126.1％、0.11cm涨幅26.8％、0.22cm涨幅73.3％、0.07cm涨幅15.6％、0.17cm增幅48.6％、0.14cm涨幅36.8％、0.13cm涨幅33.3％和0.27cm增幅108.0％，蝴蝶兰大花品种在双梗数量和诱导率方面普遍低于绒花品种，但是在花梗长度和粗度方面占据优势。
综上所述，说明统一朝向+处理空间角度处理后再辅之混合溶剂液对蝴蝶兰诱导双梗诱导起着至关重要的作用，不但可以实现“零”的突破(蝴蝶兰大花品种)，还可以明显增加双梗数量，提升诱导率。除此之外，在增长花梗长度和粗度方面也具有明显优势，对于培养出高产优质高效的蝴蝶兰，满足人们对美好生活的向往方面提供了坚实的技术基础。
以上所述仅为本发明较优的具体的实施方式，但本发明的保护范围并不局限于此，任何熟悉本技术领域的技术人员在本发明揭露的技术范围内，所作的任何修改、等同替换和改进等，应涵盖在本发明的保护范围之内。

Claims (6)

1.一种高效诱导蝴蝶兰双梗的方法，其特征在于，所述方法包括以下步骤：
步骤一、植株物理处理：将预进行催花处理的蝴蝶兰植株统一进行物理平面调整和空间角度调整；
步骤二、化学试剂处理：完成步骤一后，对蝴蝶兰植株叶片茎段喷施花梗诱导混合溶剂液；
完成蝴蝶兰双梗的高效诱导。
2.根据权利要求1所述高效诱导蝴蝶兰双梗的方法，其特征在于，所述步骤一中，物理平面调整具体操作为：将所有蝴蝶兰植株统一朝向，植株茎和叶片生长方向朝北，根部朝南。
3.根据权利要求1所述高效诱导蝴蝶兰双梗的方法，其特征在于，所述步骤一中，空间角度调整具体操作为：即将所有催花前蝴蝶兰植株统一抬高倾斜角，使植株与摇床底面呈70～90度角，光线能够由上而下照射到蝴蝶兰植株叶片3～5片的茎段叶腋处，待催花30～40d花梗长至10～20cm后去掉倾斜角恢复0度或180度摆放。
4.根据权利要求1所述高效诱导蝴蝶兰双梗的方法，其特征在于，所述步骤二中，花梗诱导混合溶剂液为：TDZ 0.01～0.05g/L+GA3 0.3～1.0g/L+IAA0.1～0.6g/L+PP3330.2～0.8g/L+P2O50.5～1.2g/L+谷氨酸0.8～1.5g/L。
5.根据权利要求1所述高效诱导蝴蝶兰双梗的方法，其特征在于，所述步骤二中，喷施的方法为每次喷施时采用小喷壶，围绕准蝴蝶兰植株叶片2～5片的茎段一周喷施，着重喷施植株叶腋处，喷完后水雾附着在茎段表面不流动为宜。
6.根据权利要求1所述高效诱导蝴蝶兰双梗的方法，其特征在于，所述步骤二中，花梗诱导混合喷施处理每9-12d一次，共计喷施2-4次。
CN202110720751.0A 2021-06-28 2021-06-28 一种高效诱导蝴蝶兰双梗的方法 Active CN113439658B (zh)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CN202110720751.0A CN113439658B (zh) 2021-06-28 2021-06-28 一种高效诱导蝴蝶兰双梗的方法
JP2023508010A JP2023537367A (ja) 2021-06-28 2022-03-25 コチョウランの双茎のための高効率誘導方法
DE112022000057.0T DE112022000057B4 (de) 2021-06-28 2022-03-25 Verfahren zur hocheffizienten Doppelstiel-Induktion von Phalaenopsis
PCT/CN2022/082907 WO2022252777A1 (zh) 2021-06-28 2022-03-25 一种高效诱导蝴蝶兰双梗的方法
GB2216951.0A GB2613254B (en) 2021-06-28 2022-03-25 Method for efficiently inducing double stems of Phalaenopsis SSP
DKPA202270523A DK181430B1 (en) 2021-06-28 2022-10-28 Method for high-efficiently inducing double stems of phalaenopsis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110720751.0A CN113439658B (zh) 2021-06-28 2021-06-28 一种高效诱导蝴蝶兰双梗的方法

Publications (2)

Publication Number Publication Date
CN113439658A true CN113439658A (zh) 2021-09-28
CN113439658B CN113439658B (zh) 2022-04-08

Family

ID=77813651

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202110720751.0A Active CN113439658B (zh) 2021-06-28 2021-06-28 一种高效诱导蝴蝶兰双梗的方法

Country Status (6)

Country Link
JP (1) JP2023537367A (zh)
CN (1) CN113439658B (zh)
DE (1) DE112022000057B4 (zh)
DK (1) DK181430B1 (zh)
GB (1) GB2613254B (zh)
WO (1) WO2022252777A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022252777A1 (zh) * 2021-06-28 2022-12-08 中国长江三峡集团有限公司 一种高效诱导蝴蝶兰双梗的方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL1030581C2 (nl) 2005-11-25 2006-05-10 Joost Petrus Jacobus Bakker Verbeterde werkwijze voor het telen van orchideeen.
CN113439658B (zh) * 2021-06-28 2022-04-08 中国长江三峡集团有限公司 一种高效诱导蝴蝶兰双梗的方法

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
YIN-TUNG WANG: "Effects of six fertilizers on vegetative growth and flowering of phalaenopsis orchids", 《SCIENTIA HORTICULTURAE》 *
YIN-TUNG WANG: "Flowering and Growth of Phalaenopsis Orchids following Growth Retardant Applications", 《HORTSCIENCE》 *
刘晓荣: "蝴蝶兰花芽分化及花期调控研究", 《中国优秀博硕士学位论文全文数据库(硕士)农业科技辑》 *
刘晓荣等: "植物生长调节剂对蝴蝶兰花芽分化与发育的影响", 《广东农业科学》 *
张文波等: "双层降温催花技术对蝴蝶兰抽梗时期及双梗率的影响", 《黑龙江农业科学》 *
曹登才等: "《家庭养花1000问》", 31 January 2011, 上海科学技术文献出版社 *
李奥等: "植物生长调节剂和温度对蝴蝶兰双梗率、花期及花朵性状的影响", 《热带作物学报》 *
金龙: "宁夏地区蝴蝶兰日光温室催花与生长控制技术研究", 《中国优秀博硕士学位论文全文数据库(硕士)农业科技辑》 *
陈冬: "南京地区蝴蝶兰生产专业化操作体系研究", 《中国优秀博硕士学位论文全文数据库(硕士)农业科技辑》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022252777A1 (zh) * 2021-06-28 2022-12-08 中国长江三峡集团有限公司 一种高效诱导蝴蝶兰双梗的方法
GB2613254A (en) * 2021-06-28 2023-05-31 China Three Gorges Corp Method for efficiently inducing double stems of Phalaenopsis SSP
GB2613254B (en) * 2021-06-28 2023-11-22 China Three Gorges Corp Method for efficiently inducing double stems of Phalaenopsis SSP

Also Published As

Publication number Publication date
DK181430B1 (en) 2023-12-06
GB202216951D0 (zh) 2022-12-28
JP2023537367A (ja) 2023-08-31
GB2613254A (en) 2023-05-31
GB2613254B (en) 2023-11-22
DE112022000057T5 (de) 2023-05-25
DK202270523A1 (en) 2023-12-06
DE112022000057B4 (de) 2024-03-14
CN113439658B (zh) 2022-04-08
WO2022252777A1 (zh) 2022-12-08

Similar Documents

Publication Publication Date Title
CN102440155B (zh) 一种兜兰花期调控方法
CN102511326B (zh) 一种盆栽桂花矮化方法
CN109089817B (zh) 一种火焰兰花期调控方法
CN105684811B (zh) 一种凤梨释迦冬期果生产的方法
CN101346062A (zh) 改进的培育兰花的方法
CN106613114B (zh) 一种秋石斛花期调控方法
CN100998298A (zh) 一种利用植物生长调节剂促进大苞鞘石斛提前开花的方法
CN107047300A (zh) 一种瓜蒌的组培育苗方法
CN108782065B (zh) 一种百香果实生苗的催花方法
CN103202152A (zh) 赏食兼用型盆栽草莓基质栽培方法
CN104920223A (zh) 一种墨兰种苗繁殖方法
CN113439658B (zh) 一种高效诱导蝴蝶兰双梗的方法
CN108739380B (zh) 一种白及组培苗一次性成苗的方法
CN106577216A (zh) 一种促进景天科多肉植物侧芽萌发的方法
CN108684511A (zh) 温室有机基质型无土栽培厚皮甜瓜的栽培方法
CN109122184B (zh) 一种秋石斛花芽诱导方法
CN106613211A (zh) 一种麝香百合北方温室栽培技术
Mu et al. Production and cultivation of lilies in China
CN101218871A (zh) 杜鹃花的选育和繁殖方法
Lobodina et al. Micropropagation of hardy Hibiscus (Hibiscus moscheutos L.)
CN103535278B (zh) 一种鸡冠花试管花卉的生产方法
CN105052705B (zh) 一种应用于昙花的育苗液体及其制备方法
CN106982660B (zh) 一种莲雾低产树形的改造方法
CN112314232B (zh) 一种调控蓝花丹花期的方法
CN111066600B (zh) 一种提高凤梨释迦反季自然座果率的方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant