JP2023537367A - コチョウランの双茎のための高効率誘導方法 - Google Patents
コチョウランの双茎のための高効率誘導方法 Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 18
- 230000006698 induction Effects 0.000 title claims description 60
- 230000001939 inductive effect Effects 0.000 claims abstract description 21
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims abstract description 10
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims abstract description 8
- 235000013922 glutamic acid Nutrition 0.000 claims abstract description 8
- 239000004220 glutamic acid Substances 0.000 claims abstract description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 7
- 244000127818 Phalaenopsis amabilis Species 0.000 claims description 44
- 241000196324 Embryophyta Species 0.000 claims description 34
- 239000011259 mixed solution Substances 0.000 claims description 23
- 238000005507 spraying Methods 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 230000012010 growth Effects 0.000 claims description 3
- 230000001965 increasing effect Effects 0.000 abstract description 14
- 238000003976 plant breeding Methods 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 abstract 1
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- 241000894007 species Species 0.000 description 24
- 238000012360 testing method Methods 0.000 description 24
- 238000007619 statistical method Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 3
- UUTKICFRNVKFRG-WDSKDSINSA-N (4R)-3-[oxo-[(2S)-5-oxo-2-pyrrolidinyl]methyl]-4-thiazolidinecarboxylic acid Chemical compound OC(=O)[C@@H]1CSCN1C(=O)[C@H]1NC(=O)CC1 UUTKICFRNVKFRG-WDSKDSINSA-N 0.000 description 2
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- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 1
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- 230000005923 long-lasting effect Effects 0.000 description 1
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- 230000005082 stem growth Effects 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
- 241000233856 x Doritaenopsis Species 0.000 description 1
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Abstract
Description
催花を実行しようとするコチョウランの植物体に対し物理的平面と空間角度を揃えて調整する物理的処理S1と、
S1終了後、コチョウランの第2~5葉の茎部に10日間毎に1回から計3回花柄誘導混合溶液を噴霧する化学試薬による処理S2と、
コチョウランの双茎のための高効率誘導を完了する処理とを含む。
1、屋外で植物体を栽培できるため、簡単な操作性と顕著な効果を備え、コチョウランの双茎を定期的に誘導し出すことができる。
1、物理的処理
双茎の萌出を順調且つ有効的に促すために、まずコチョウランの植物体に対し物理的処理を行い、主に催花を実行しようとするコチョウランの植物体に対し物理的平面と空間角度とを揃えて調整する。一番目には、物理的平面を調整する、即ち、全てのコチョウランの植物体の向きが整い揃っており、本実験温室が南北方向に合わされており(その他類似の温室が実際の状況に応じて調整できる)、植物体の茎及び葉の生長方向を北に向けて根部を南に向けて配置することで植物体が地球磁場の方向と一致することを確かにすることにより、地球磁場を植物体に通過させ、植物体に対する地球磁場の影響を最小限に抑えるだけでなく、施肥や灌水や植え替えなどの操作を容易にすることができる(図1をご覧ください)。二番目には、向きが整い揃った後に植物体に対して空間角度を調整する、即ち、催花を実行しようとする全てのコチョウランの植物体を同じ傾斜角に挙げ、当該植物体の第3~5葉の茎部にある葉腋に光が上から下に当たるように植物体を揺れベッドの底面に向かって70~90度に傾斜させ、催花を30~40日間行って小花柄が10~20cmに生長した後、傾斜角を無くして0度又は180度に復して植物体を置く(図2をご覧ください)。詳細な実験結果を表1~3に示す。
化学試薬による処理についての研究は、向きが整い揃い且つ空間角度処理を行った後、コチョウランの第2~5葉の茎部に花柄誘導混合溶液(TDZ(0.01~0.05g/L)+GA3(0.3~1.0g/L)+IAA(0.1~0.6g/L)+PP333(0.2~0.8g/L)+P2O5(0.5~1.2g/L)+グルタミン酸(0.8~1.5g/L))を噴霧するとともに対照群(花柄誘導混合溶液を噴霧せず)を配置することである。各処理工程に30鉢の植物体があり、実験を3回繰り返す必要がある。花柄誘導混合溶液を10日間ごとに1回、計3回噴霧する必要がある。噴霧方法は、水玉が茎部の表面に付着して流れないほどを好適とするように、毎回150mlの小型如雨露を用いて最良の噴霧状態に壷口を調整してコチョウランの植物体の第2~5葉の茎部を一周回るとともに噴霧を行い、特に植物体の葉腋に噴霧することである。実験結果は、花柄誘導混合溶液を噴霧した植物体の誘導率について、小花品種が98.9%で、大花品種が88.9%で、小花品種は大花品種に比べて10%増え、小花品種と大花品種の平均双茎率は93.9%であるが、花柄誘導混合溶液を噴霧しなかった植物体の双茎率は低くなることを明らかにする。詳細な実験結果を表4に示す。
[付記1]
コチョウランの双茎の高効率誘導方法であって、
催花を実行しようとするコチョウランの植物体について物理的平面と空間角度を揃えて調整する物理的処理S1と、
S1終了後、コチョウランの葉の茎部に花柄誘導混合溶液を噴霧する化学試薬による処理S2と、
コチョウランの双茎の高効率誘導を完了する処理とを含み、
S1において、物理的平面を調整する工程は、具体的に、全てのコチョウランの植物体の向きが整い揃い、それらの茎及び葉の生長方向を北に向け、それらの根部を南に向けて配置するようにさせることであり、
S1において、空間角度を調整する工程は、具体的に、コチョウランの植物体の第3~5葉の茎部にある葉腋に光が上から下に当たるように催花を実行しようとする全てのコチョウランの植物体を同じ傾斜角に挙げ、催花を30~40日間行って小花梗が10~20cmに生長した後に、傾斜角を無くして0度又は180度に復して植物体を置くことであり、
S2において、花柄誘導混合溶液はTDZ(0.01~0.05g/L)+GA3(0.3~1.0g/L)+IAA(0.1~0.6g/L)+PP333(0.2~0.8g/L)+P2O5(0.5~1.2g/L)+グルタミン酸(0.8~1.5g/L)を含む
ことを特徴とする方法。
S2において、噴霧方法は、水玉が茎部の表面に付着して流れないほどを好適とするように、毎回小型如雨露を用いてコチョウランの植物体の第2~5葉の茎部を一周回るとともに噴霧を行い、特に植物体の葉腋に噴霧することである
ことを特徴とする付記1に記載の方法。
S2において、花柄誘導混合溶液による噴霧処理は9~12日間ごとに一回行われ、計2~4回実行される
ことを特徴とする付記1に記載の方法。
Claims (3)
- コチョウランの双茎の高効率誘導方法であって、
催花を実行しようとするコチョウランの植物体について物理的平面と空間角度を揃えて調整する物理的処理S1と、
S1終了後、コチョウランの葉の茎部に花柄誘導混合溶液を噴霧する化学試薬による処理S2と、
コチョウランの双茎の高効率誘導を完了する処理とを含み、
S1において、物理的平面を調整する工程は、具体的に、全てのコチョウランの植物体の向きが整い揃い、それらの茎及び葉の生長方向を北に向け、それらの根部を南に向けて配置するようにさせることであり、
S1において、空間角度を調整する工程は、具体的に、コチョウランの植物体の第3~5葉の茎部にある葉腋に光が上から下に当たるように催花を実行しようとする全てのコチョウランの植物体を同じ傾斜角に挙げ、催花を30~40日間行って小花梗が10~20cmに生長した後に、傾斜角を無くして0度又は180度に復して植物体を置くことであり、
S2において、花柄誘導混合溶液はTDZ(0.01~0.05g/L)+GA3(0.3~1.0g/L)+IAA(0.1~0.6g/L)+PP333(0.2~0.8g/L)+P2O5(0.5~1.2g/L)+グルタミン酸(0.8~1.5g/L)を含む
ことを特徴とする方法。 - S2において、噴霧方法は、水玉が茎部の表面に付着して流れないほどを好適とするように、毎回小型如雨露を用いてコチョウランの植物体の第2~5葉の茎部を一周回るとともに噴霧を行い、特に植物体の葉腋に噴霧することである
ことを特徴とする請求項1に記載の方法。 - S2において、花柄誘導混合溶液による噴霧処理は9~12日間ごとに一回行われ、計2~4回実行される
ことを特徴とする請求項1に記載の方法。
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CN113439658B (zh) * | 2021-06-28 | 2022-04-08 | 中国长江三峡集团有限公司 | 一种高效诱导蝴蝶兰双梗的方法 |
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CN113439658A (zh) | 2021-09-28 |
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