CN113398057A - Bacteriostatic agent and preparation method thereof - Google Patents

Bacteriostatic agent and preparation method thereof Download PDF

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CN113398057A
CN113398057A CN202011532873.9A CN202011532873A CN113398057A CN 113398057 A CN113398057 A CN 113398057A CN 202011532873 A CN202011532873 A CN 202011532873A CN 113398057 A CN113398057 A CN 113398057A
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parts
phase
bacteriostatic agent
cooling
steam
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梁正强
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Chongqing Zhongjiaxin Health Management Co Ltd
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Chongqing Zhongjiaxin Health Management Co Ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/99Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34Alcohols
    • A61K8/345Alcohols containing more than one hydroxy group
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/58Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing atoms other than carbon, hydrogen, halogen, oxygen, nitrogen, sulfur or phosphorus
    • A61K8/585Organosilicon compounds
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64Proteins; Peptides; Derivatives or degradation products thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/65Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67Vitamins
    • A61K8/673Vitamin B group
    • A61K8/675Vitamin B3 or vitamin B3 active, e.g. nicotinamide, nicotinic acid, nicotinyl aldehyde
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/72Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/84Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
    • A61K8/89Polysiloxanes
    • A61K8/891Polysiloxanes saturated, e.g. dimethicone, phenyl trimethicone, C24-C28 methicone or stearyl dimethicone
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9789Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00Drugs for genital or sexual disorders; Contraceptives
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00Drugs for genital or sexual disorders; Contraceptives
    • A61P15/02Drugs for genital or sexual disorders; Contraceptives for disorders of the vagina
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/02Local antiseptics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005Antimicrobial preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • FMECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F26DRYING
    • F26BDRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
    • F26B21/00Arrangements or duct systems, e.g. in combination with pallet boxes, for supplying and controlling air or gases for drying solid materials or objects
    • F26B21/005Drying-steam generating means
    • FMECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F26DRYING
    • F26BDRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
    • F26B25/00Details of general application not covered by group F26B21/00 or F26B23/00
    • F26B25/005Treatment of dryer exhaust gases
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Abstract

The invention discloses a bacteriostatic agent which comprises the following components in parts by weight: 10-50 parts of water, 0.05-0.2 part of xanthan gum, 0.1-0.5 part of carbomer, 10-20 parts of glycerol, 0.2-1 part of turmeric gum, 2-10 parts of collagen, 0.05-0.2 part of propyl hydroxybenzoate and 0.1-0.4 part of methyl hydroxybenzoate; phase B: 10-20 parts of liquid paraffin, 1-3 parts of ethyl hexyl palmitate, 1-3 parts of isooctyl palmitate, 1-3 parts of DC-345 cyclopentasiloxane, 1-3 parts of cyclohexasiloxane, 1-3 parts of polydimethylsiloxane, 1-3 parts of glyceryl monostearate and a C phase: 0.1-0.5 part of triethanolamine, 0.3-1 part of plant anti-allergy agent, 1-2 parts of nicotinic acid derivative, 1-3 parts of antibacterial peptide solution, 0.1-0.4 part of phenoxyethanol, 0.05-0.15 part of chlorphenesin and 0.05-0.15 part of 1,2 hexanediol. The bacteriostatic agent has the advantages of good bactericidal effect and capability of improving the immunity of the organism.

Description

Bacteriostatic agent and preparation method thereof
Technical Field
The invention relates to the technical field of bacteriostatic agents, in particular to a bacteriostatic agent.
Background
According to hospital data statistics, the number of female reproductive system diseases is increased year by year in recent years, the bacteriostatic agent on the market only has the functions of sterilization and inflammation diminishing, the components of the bacteriostatic agent have high irritation to the reproductive system and high toxic and side effects, the immunity of the reproductive system can be influenced after long-time use, good bacteriostatic protection is difficult to form after the bacteriostatic action is performed, and the bacteriostatic agent has high drug dependence.
Therefore, those skilled in the art have been devoted to develop a bacteriostatic agent which can enhance the immunity of the reproductive system itself while sterilizing.
Disclosure of Invention
In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is to provide a bacteriostatic agent, which has solved the problem that the bacteriostatic agent in the prior art only inhibits bacteria, but cannot improve the immunity of the reproductive system of the user.
In order to achieve the purpose, the invention provides a bacteriostatic agent which comprises the following components in parts by weight: 10-50 parts of water, 0.05-0.2 part of xanthan gum, 0.1-0.5 part of carbomer, 10-20 parts of glycerol, 0.2-1 part of turmeric gum, 2-10 parts of collagen, 0.05-0.2 part of propyl hydroxybenzoate and 0.1-0.4 part of methyl hydroxybenzoate; phase B: 10-20 parts of liquid paraffin, 1-3 parts of ethyl hexyl palmitate, 1-3 parts of isooctyl palmitate, 1-3 parts of DC-345 cyclopentasiloxane, 1-3 parts of cyclohexasiloxane, 1-3 parts of polydimethylsiloxane, 1-3 parts of glyceryl monostearate and a C phase: 0.1-0.5 part of triethanolamine, 0.3-1 part of plant anti-allergy agent, 1-2 parts of nicotinic acid derivative, 1-3 parts of antibacterial peptide solution, 0.1-0.4 part of phenoxyethanol, 0.05-0.15 part of chlorphenesin and 0.05-0.15 part of 1,2 hexanediol.
Furthermore, the phase A also comprises 5-10 parts of pepper extract.
Further, in the phase A, 44.3 parts of water, 0.1 part of xanthan gum, 0.3 part of carbomer, 15 parts of glycerol, 0.5 part of turmeric gum, 5 parts of collagen, 0.1 part of hydroxy phenylpropyl ester, 0.2 part of hydroxymethyl ester and 8 parts of pepper extract are contained in the phase A.
Further, in the B phase, 15 parts of liquid paraffin, 2.5 parts of ethyl hexyl palmitate, 2.5 parts of isooctyl palmitate, 2.5 parts of DC-345 cyclopentasiloxane, 2.5 parts of cyclohexasiloxane, 2 parts of polydimethylsiloxane and 2 parts of glycerol monostearate were used.
Further, in the phase C, 0.3 part of triethanolamine, 0.5 part of plant anti-allergic agent, 1.5 parts of nicotinic acid derivative and 2 parts of antibacterial peptide solution are added; 0.2 part of phenoxyethanol; 0.1 part of chlorphenesin; 0.1 part of 1,2 hexanediol;
further, the antibacterial peptide solution comprises 0.5 part of bacillus fermentation product, 0.5 part of propylene glycol, 0.5 part of hexylene glycol and 0.5 part of water.
Further, the pepper extract is pepper drying steam condensate.
A preparation method of a bacteriostatic agent comprises (1) mixing and soaking phase A for 20min, heating to 85 deg.C, stirring to dissolve completely, and keeping the temperature for 20 min; (2) mixing the phase B, heating to 85 ℃, adding the phase B into the phase A, stirring, and completing emulsification to obtain a mixed emulsion; (3) cooling the mixed emulsion obtained in the step (2) to 45 ℃; (4) mixing the phase C and adding into the mixed emulsion; (5) and cooling to normal temperature and discharging.
A prickly ash drying steam recovery system for preparing a prickly ash extract comprises: the pepper dryer is used for drying wet peppers and comprises a shell, wherein a fan and a heating plate are arranged in the shell, a heater is arranged in the heating plate, the fan is arranged at the bottom of the heating plate, and a plurality of ventilation holes are formed in the heating plate; the steam collecting pipe is used for collecting steam generated by drying, is arranged in the vertical direction and is connected to the top of the shell; a plurality of cooling plates are arranged in the steam collecting pipe, the plurality of cooling plates form an S-shaped channel, each cooling plate is connected with a liquid inlet pipe and a liquid outlet pipe, a plurality of cooling channels are arranged in the cooling plates, and two ends of each cooling channel are communicated with the liquid inlet pipe and the liquid outlet pipe; the condensate collecting box is used for collecting condensate of steam dried by the pepper and is arranged at the bottom of the steam collecting pipe and below the bottommost cooling plate;
furthermore, the cooling plate comprises an upper plate and a lower plate which are the same in structure, a plurality of arc-shaped liquid tanks are arranged on the upper plate and the lower plate, two ends of each liquid tank are open, and the liquid tanks after the upper plate and the lower plate are attached form the cooling channel.
The invention has the beneficial effects that: the invention adopts low-irritation raw materials and various herbaceous plant raw materials, has good bacteriostatic and anti-inflammatory functions, can improve the immunity of a reproductive system during use, and avoids generating drug dependence. Meanwhile, the bacteriostatic agent disclosed by the invention is low in toxic and side effects, does not contain heavy metal components, and has no irritation and toxic and side effects, so that the use comfort is improved, the product is good in stability, and the using effect is not easily influenced by deterioration.
The prickly ash extract in this scheme is prickly ash stoving steam condensate, the stoving steam that does not need in the dry prickly ash preparation technology has been utilized, condensate after the steam condensation uses, can carry out effectual recovery and utilization to the resource like this, its composition acquires the convenience, and its antibacterial effect is also better, prickly ash stoving steam condensate is prepared through a prickly ash stoving steam recovery system in this scheme, carry out the condensation and acquire the condensate with steam recovery when drying the prickly ash, and simple process is practical.
Drawings
FIG. 1 is a schematic structural diagram of a pepper drying steam recovery system;
FIG. 2 is a schematic view of a cooling plate in a vapor recovery system for drying peppers;
fig. 3 is a schematic view of a-a in fig. 2.
Detailed Description
Example one
A bacteriostatic agent comprises the following components in parts by weight:
phase A: 44.3 parts of water; 0.1 part of xanthan gum; 0.3 part of carbomer; 15 parts of glycerol; 0.5 part of turmeric glue; 5 parts of collagen; 0.1 part of propyl hydroxybenzoate; 0.2 part of methylparaben; and 8 parts of pepper extract.
Phase B: 15 parts of liquid paraffin; 2.5 parts of ethyl hexyl palmitate; 2.5 parts of isooctyl palmitate; 2.5 parts of DC-345 cyclopentasiloxane; 2.5 parts of cyclohexasiloxane; 2 parts of polydimethylsiloxane; and 2 parts of glyceryl monostearate.
And C phase: 0.3 part of triethanolamine; 0.5 part of plant anti-allergy agent, wherein the plant anti-allergy agent is dioscorea longissima root extract; 1.5 parts of a nicotinic acid derivative; 2 parts of antibacterial peptide liquid, wherein the antibacterial peptide liquid comprises 0.5 part of bacillus fermentation product, 0.5 part of propylene glycol, 0.5 part of hexanediol and 0.5 part of water; 0.2 part of phenoxyethanol; 0.1 part of chlorphenesin; 0.1 part of 1,2 hexanediol.
Example two
Phase A: 10 parts of water; 0.05 part of xanthan gum; 0.1 part of carbomer; 10 parts of glycerol; 0.2 part of turmeric glue; 2 parts of collagen; 0.05 part of propyl hydroxybenzoate; 0.1 part of methylparaben; 5 parts of pepper extract
Phase B: 10 parts of liquid paraffin; 1 part of ethyl hexyl palmitate; 1 part of isooctyl palmitate; 1 part of DC-345 cyclopentasiloxane; 1 part of cyclohexasiloxane; 1 part of polydimethylsiloxane; and 1 part of glycerin monostearate.
And C phase: 0.1 part of triethanolamine; 0.3 part of plant anti-allergy agent, wherein the plant anti-allergy agent is dioscorea longissima root extract; 1 part of nicotinic acid derivative; 1 part of antibacterial peptide solution; wherein the antibacterial peptide solution comprises 0.5 part of bacillus fermentation product, 0.2 part of propylene glycol, 0.2 part of hexanediol and 0.1 part of water; 0.1 part of phenoxyethanol; 0.05 part of chlorphenesin; 0.05 part of 1,2 hexanediol.
EXAMPLE III
Phase A: 50 parts of water; 0.2 part of xanthan gum; 0.5 part of carbomer; 20 parts of glycerol; 1 part of turmeric gum; 10 parts of collagen; 0.2 part of propyl hydroxybenzoate; 0.4 part of methylparaben; 10 parts of pepper extract.
Phase B: 20 parts of liquid paraffin; 3 parts of ethyl hexyl palmitate; 3 parts of isooctyl palmitate; 3 parts of DC-345 cyclopentasiloxane; 3 parts of cyclohexasiloxane; 3 parts of polydimethylsiloxane; and 3 parts of glycerin monostearate.
And C phase: 0.5 part of triethanolamine; 1 part of plant anti-allergy agent, wherein the plant anti-allergy agent is a long-hair dioscorea root extract; 2 parts of a nicotinic acid derivative; 3 parts of antibacterial peptide liquid, wherein the antibacterial peptide liquid comprises 0.8 part of bacillus fermentation product, 0.8 part of propylene glycol, 0.8 part of hexanediol and 0.6 part of water; 0.4 part of phenoxyethanol; 0.15 part of chlorphenesin; 0.15 part of 1,2 hexanediol.
English names of part of raw materials in the examples: glycerin, liquid paraffin Paraffin Liquidum, XANTHAN GUM, CARBOMER Carbomer, DC-345 CYCLOPENTASILOXANE and cyclohexasiloxane cyclopropenesine, ETHYLHEXYL PALMITATE/isooctyl PALMITATE hydroxy PALMITATE, DIMETHICONE dimethylsiloxane DIMETHICONE, Glyceryl monostearate, TRIETHANOLAMINE, PHENOXYETHANOL phenoxy phenoxythane, CHLORPHENESIN, 1,2 HEXANEDIOL, 1,2-HEXANEDIOL, the plant anti-sensate diospore VILLOSA (WILD YAM) ROOT EXTRACT, nicotinic acid derivative NIACINAMIDE, Bacillus fermentation product BACILLUS FEENT, COLLAGEN protein HYDROXYLYZED COLLAGEN, METHYLPARABEN, PROPYLPARABEN pyrrolidone.
A preparation method of a bacteriostatic agent comprises the following steps according to the formula of the first to third embodiments:
(1) mixing and soaking the phase A for 20 minutes, heating to 85 ℃, stirring until the phase A is completely dissolved, and keeping the temperature for 20 minutes;
(2) mixing and heating the phase B to 85 ℃, adding the phase B into the phase A, stirring, and completing emulsification to obtain a mixed emulsion;
(3) cooling the mixed emulsion obtained in the step (2) to 45 ℃;
(4) mixing the phase C and adding into the mixed emulsion;
(5) and cooling to normal temperature and discharging.
The pepper extract is specifically pepper drying steam condensate, and the pepper drying steam condensate is mainly adopted in the pepper drying process in the manufacturing process of the dried pepper. After picking and cleaning fresh pepper, draining excessive water, putting the fresh pepper into a pepper dryer for drying, generating steam in the drying process, and condensing the recovered steam to obtain condensate, namely the pepper extract in the first to third embodiments.
The bacteriostatic performance test of the bacteriostatic agent in example one is as follows:
first, equipment
1. Test strains: coli ATCC 25922, staphylococcus aureus ATCC6538, candida albicans ATCC10231, passage 4 fresh culture.
2. Test samples: the bacteriostatic agent of the first embodiment.
Second, method
1. The detection basis is as follows: GB15979-2002 hygienic Standard appendix C4 of Disposable sanitary articles.
2. Detection conditions are as follows: the test temperature was 24 ℃ and the humidity was 52%.
3. The experiment was repeated 3 times.
Three, result in
(1) After 3 times of repeated tests, the results show that the average values of the bacteriostasis rates of the test sample stock solution on escherichia coli for 2min, 5min, 10min and 20min are all more than 50.0%, and the results are shown in table 1.
Table 1 shows the bacteriostatic effect of the bacteriostatic agent of example one on Escherichia coli
Figure BDA0002852536780000061
Figure BDA0002852536780000071
(2) After 3 times of repeated tests, the results show that the average values of the bacteriostasis rates of the test sample stock solution on staphylococcus aureus in 2min, 5min, 10min and 20min are all more than 90%, which is shown in table 2.
Table 2 shows the bacteriostatic effect of the bacteriostatic agent of the first embodiment on staphylococcus aureus
Figure BDA0002852536780000072
(3) After 3 times of repeated tests, the results show that the average values of the bacteriostasis rates of the test sample stock solution on the Candida albicans for 2min, 5min, 10min and 20min are all more than 99.9%, and the results are shown in Table 3.
Table 3 shows the bacteriostatic effect of the bacteriostatic agent of the first embodiment on Candida albicans
Figure BDA0002852536780000073
Figure BDA0002852536780000081
Fourth, conclusion
After 3 times of repeated tests, the result shows that the raw solution of the argyi lanugo bacteriostatic agent has the bactericidal rate of 50.0% for escherichia coli, staphylococcus aureus and candida albicans in 2min, 5min, 10min and 20min, has strong bacteriostatic action, and meets the requirement that the product has strong bacteriostatic action in GB15979-2002, appendix C4.2 of hygienic standard GB15979-2002 for disposable sanitary articles is more than or equal to 50-90%.
The toxicological test for the bacteriostatic agent of example one is as follows
Materials and animals
1. And (3) testing a sample:
1.1 sample description: the bacteriostatic agent of the first embodiment.
1.2 sample preparation: the original test substance.
2. Animal and feeding environment:
Figure BDA0002852536780000082
Figure BDA0002852536780000091
second, method
1. The detection basis is as follows: GB15979-2002
2. Number and group of experimental animals: the test is divided into a virus infection group and a control group, and each group comprises 3 animals.
3. The operation procedure is as follows:
3.1 fixing the rabbit on the back, exposing perineum and vaginal orifice. A catheter (a child catheter having a length of about 8cm and connected to a 5mL syringe) was wetted with the test substance, gently inserted into the vagina (4cm), 2mL of the test substance was slowly injected with the syringe, and the catheter was withdrawn. The animals in the control group were treated with physiological saline in the same manner;
3.2 after the infection, the animals are killed by an air embolism method 24h, the whole vagina is taken out after the laparotomy, the longitudinal dissection is carried out, whether congestion, edema and other manifestations exist is observed by naked eyes, then the vagina is placed into 10% formalin solution for fixation for 48h, tissues at two ends and 3 middle parts of the vagina are selected for flaking, HE staining is carried out, and histopathology examination is carried out.
4. The result evaluation method comprises the following steps:
4.1 scoring the histopathological examination results;
4.2 the stimulation response integrals of 3 parts of 3 animals in the test group are added and then divided by the total number of observation to obtain the average integral of the vaginal mucosa stimulation response in the test group. The control group scoring method is the same as above;
4.3 the average integral of the test group is subtracted by the average integral of the control group to obtain the stimulation index, and then the stimulation intensity is graded.
Third, test results
The vaginal mucosal irritation response scores are given in table 4 below.
Vaginal mucosa irritation response scores summary table 4
Figure BDA0002852536780000092
Figure BDA0002852536780000101
Note: 1) the average integral is the sum of the stimulation response integrals of 3 sites of 3 animals/total observed number (animals x 3); 2) stimulation index is the mean integral of the infected group-the mean integral of the negative control group.
Fourthly, conclusion:
the stimulation index of the sample to the vaginal mucosa of the rabbit is 0.22, and the stimulation response intensity of the vaginal mucosa is the stimulation of the vagina-free mucosa.
The following is a heavy metal test for the bacteriostatic agent of example one:
first, equipment
1. Sample name: the embodiment is a bacteriostatic agent.
2. The instrument equipment comprises: electronic balance, model BSA 124S: microwave digestion instrument, model number TOPEX: inductively coupled plasma mass spectrometry (ICP-WS) model Nexlon 350X.
3. Concentration of standard solution: lead standard solution (1 mg/L); arsenic standard solution (1 mg/L); mercury standard solution (1 mg/L).
Second, method
1. The detection basis is as follows: chapter iv physical and chemical inspection method 1.6 of cosmetic safety specifications (2015 edition).
2. The detection environment temperature is 23.6 ℃, and the humidity is 35%.
Three, result in
EXAMPLES results of measurement of lead, arsenic and mercury as bacteriostatic agents
Detecting items The result of the detection
Lead (II) Not detected (<0.09mg/L)
Arsenic (As) Not detected (<0.0033mg/L)
Mercury Not detected (<0.0033mg/L)
The following is a stability test for the bacteriostatic agent of example one:
after the bacteriostatic agent in the first embodiment is stored in a constant-temperature constant-humidity box with the relative humidity of more than 75% at 37 ℃ for 3 months, the appearance and the package of the bacteriostatic agent are not obviously changed, and after 3 times of repeated tests, the bacteriostatic agent in the first embodiment has stronger bacteriostatic action on escherichia coli, staphylococcus aureus and candida albicans within 2min, 5min, 10min and 20min, meets the requirement of stronger bacteriostatic action on products which are more than or equal to 90% in the appendix C4.2 of GB15979-2002 hygienic Standard for Disposable use, and the bacteriostatic action of the bacteriostatic agent is maintained at room temperature for at least two years.
The following is a microbiological indicator test of the bacteriostatic agent of example one:
first, equipment
1. Test samples: the embodiment is a bacteriostatic agent.
Second, method
1. The detection basis is as follows: the hygienic standard GB15979-2002 appendix B of the disposable hygienic article.
2. The test temperature and humidity were: 21 ℃ and 55 percent.
Three, result in
EXAMPLE results of microbiological indicators for bacteriostatic agents
Figure BDA0002852536780000111
Figure BDA0002852536780000121
A prickly ash drying steam recovery system for preparing the prickly ash extract comprises a prickly ash dryer 1, a steam collecting pipe 2 and a condensate collecting box 3. The pepper dryer 1 is used for drying fresh and wet pepper, and can dry the fresh and wet pepper into dry pepper; the steam collecting pipe 2 is used for collecting steam generated in the process of drying the fresh and wet pepper and condensing the steam into condensate; and the condensate collecting box 3 is used for collecting pepper drying steam condensate.
Prickly ash drying-machine 1 includes casing 4, be equipped with fan 5 and hot plate 6 in the casing 4, the intermediate position in casing 4 is established to hot plate 6 level, and fan 5 is established in the bottom of casing 4, the below of hot plate 6, and the vertical upwards bottom towards hot plate 6 of air outlet of fan 5. The heating plate 6 is provided with a heater 13, and particularly, the heater 13 is an electric heater. Be equipped with a plurality of ventilation holes on the hot plate 6, specific hot plate 6 is the orifice plate, and the ventilation hole equipartition is arranged and is seted up on hot plate 6, and bright wet prickly ash is placed on hot plate 6, and the granule of bright wet prickly ash can not follow the ventilation hole and drop.
Steam collecting pipe 2 connects in the terminal top of 4 casings of prickly ash drying-machine 1, through the vertical upwards setting of one end return bend, be equipped with a plurality of cooling plates 7 in the steam collecting pipe 2, specifically be 4 cooling plates 7 in this embodiment, cooling plates 7 divide 4 layers to fix in turn and set up on steam collecting pipe 2' S inner wall, and 4 cooling plates 7 form an S-shaped passageway. Each cooling plate 7 is disposed horizontally inclined downward. The tail end of the steam collecting pipe 2 is provided with an induced draft fan 8, and the induced draft fan 8 is used for enhancing the flow of steam and plays a role in drainage.
Be equipped with a plurality of cooling channel 9 in the cooling plate 7, cooling channel 9 is the arc structure, and the entry and the export of cooling channel 9 all are established on the same board edge of cooling plate 7, specifically are the fixed edge of the inner wall of cooling plate 7 and steam collecting pipe 2. The specific quantity of cooling channel 9 is 3, and 3 cooling channel 9 include 3 entrances and 3 exports, and 3 entrances and 3 exports connect external water pipe 12 after connecting a cross 10 respectively through connecting water pipe 11 jointly.
The cooling plate 7 is formed by jointing an upper plate 7-1 and a lower plate 7-2 which have the same structure, a plurality of arc-shaped liquid grooves are formed in the upper plate and the lower plate, the liquid grooves formed after the upper plate and the lower plate are jointed form the cooling channel 9, and the jointing part of the upper plate and the lower plate is sealed in a waterproof way through sealant.
And the condensate collecting box 3 is arranged at the bottom of the steam collecting pipe 2 and below the bottommost cooling plate 7. The steam is cooled when meeting the cooling plate 7, and the steam is condensed into condensate which drops into the condensate collecting box 3 and is collected for standby.
The foregoing detailed description of the preferred embodiments of the invention has been presented. It should be understood that numerous modifications and variations could be devised by those skilled in the art in light of the present teachings without departing from the inventive concepts. Therefore, the technical solutions available to those skilled in the art through logic analysis, reasoning and limited experiments based on the prior art according to the concept of the present invention should be within the scope of protection defined by the claims.

Claims (10)

1. The bacteriostatic agent is characterized by comprising the following components in parts by weight:
phase A: 10-50 parts of water;
0.05-0.2 part of xanthan gum;
0.1-0.5 part of carbomer;
10-20 parts of glycerol;
0.2-1 part of turmeric gum;
2-10 parts of collagen;
0.05-0.2 part of propyl hydroxybenzoate;
0.1-0.4 part of methylparaben;
phase B: 10-20 parts of liquid paraffin;
1-3 parts of ethyl hexyl palmitate;
1-3 parts of isooctyl palmitate;
1-3 parts of DC-345 cyclopentasiloxane;
1-3 parts of cyclohexasiloxane;
1-3 parts of polydimethylsiloxane;
1-3 parts of glycerin monostearate;
and C phase: 0.1-0.5 part of triethanolamine;
0.3-1 part of plant anti-allergic agent;
1-2 parts of a nicotinic acid derivative;
1-3 parts of antibacterial peptide solution;
0.1-0.4 part of phenoxyethanol;
0.05-0.15 part of chlorphenesin;
0.05-0.15 part of 1,2 hexanediol.
2. A bacteriostatic agent according to claim 1, characterized in that: and the phase A also comprises 5-10 parts of pepper extract.
3. A bacteriostatic agent according to claims 1 and 2, characterized in that: in the phase A, 44.3 parts of water, 0.1 part of xanthan gum, 0.3 part of carbomer, 15 parts of glycerol, 0.5 part of turmeric gum, 5 parts of collagen, 0.1 part of hydroxy phenyl benzoate, 0.2 part of methyl hydroxybenzoate and 8 parts of pepper extract.
4. A bacteriostatic agent according to claim 1, characterized in that: in the phase B, 15 parts of liquid paraffin, 2.5 parts of ethyl hexyl palmitate, 2.5 parts of isooctyl palmitate, 2.5 parts of DC-345 cyclopentasiloxane, 2.5 parts of cyclohexasiloxane, 2 parts of polydimethylsiloxane and 2 parts of glycerol monostearate.
5. A bacteriostatic agent according to claim 1, characterized in that: in the phase C, 0.3 part of triethanolamine, 0.5 part of plant anti-allergic agent, 1.5 parts of nicotinic acid derivative and 2 parts of antibacterial peptide solution are added; 0.2 part of phenoxyethanol; 0.1 part of chlorphenesin; 0.1 part of 1,2 hexanediol.
6. A bacteriostatic agent according to claim 5, which is characterized in that: the antibacterial peptide solution comprises 0.5 part of bacillus fermentation product, 0.5 part of propylene glycol, 0.5 part of hexanediol and 0.5 part of water.
7. A bacteriostatic agent according to claim 1, characterized in that: the fructus Zanthoxyli extract is fructus Zanthoxyli drying steam condensate.
8. A method for preparing a bacteriostatic agent according to any one of claims 1 to 7, characterized by comprising the following steps:
(1) mixing and soaking the phase A for 20 minutes, heating to 85 ℃, stirring until the phase A is completely dissolved, and keeping the temperature for 20 minutes;
(2) mixing and heating the phase B to 85 ℃, adding the phase B into the phase A, stirring, and completing emulsification to obtain a mixed emulsion;
(3) cooling the mixed emulsion obtained in the step (2) to 45 ℃;
(4) mixing the phase C and adding into the mixed emulsion;
(5) and cooling to normal temperature and discharging.
9. A pepper drying vapor recovery system for preparing the pepper extract of claim 2, comprising:
the pepper dryer is used for drying wet peppers and comprises a shell, wherein a fan and a heating plate are arranged in the shell, a heater is arranged in the heating plate, the fan is arranged at the bottom of the heating plate, and a plurality of ventilation holes are formed in the heating plate;
the steam collecting pipe is used for collecting steam generated by drying, is arranged in the vertical direction and is connected to the top of the shell; a plurality of cooling plates are arranged in the steam collecting pipe, the plurality of cooling plates form an S-shaped channel, each cooling plate is connected with a liquid inlet pipe and a liquid outlet pipe, a plurality of cooling channels are arranged in the cooling plates, and two ends of each cooling channel are communicated with the liquid inlet pipe and the liquid outlet pipe;
and the condensate collecting box is used for collecting pepper drying steam condensate, is arranged at the bottom of the steam collecting pipe and is arranged below the bottommost cooling plate.
10. The pepper drying steam recovery system as defined in claim 9, wherein: the cooling plate comprises an upper plate and a lower plate which are the same in structure, a plurality of arc-shaped liquid tanks are arranged on the upper plate and the lower plate, two ends of each liquid tank are open, and the liquid tanks after the upper plate and the lower plate are attached form the cooling channel.
CN202011532873.9A 2020-12-22 2020-12-22 Bacteriostatic agent and preparation method thereof Pending CN113398057A (en)

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CN110812283A (en) * 2019-12-17 2020-02-21 广州市澳莱化妆品有限公司 Anti-inflammation sterilization makeup removing cream and preparation method thereof
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102416011A (en) * 2011-10-10 2012-04-18 艾硕特生物科技(昆明)有限公司 Broad-spectrum and high-efficiency antibacterial washing liquor
CN105288393A (en) * 2015-12-08 2016-02-03 刘锋 Antibacterial cream for skin and preparation method of antibacterial cream
CN105411873A (en) * 2015-12-14 2016-03-23 北京肽康生物科技有限公司 Application of antibacterial peptide as anti-inflammation acne removal agent for cosmetic preparation
CN106974857A (en) * 2017-03-28 2017-07-25 佛山市汇汾化妆品科技有限公司 A kind of antibacterial mildy wash
CN106924121A (en) * 2017-04-27 2017-07-07 广州巴宝莉化妆品有限公司 A kind of long-acting bacteriostatic skin cream
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CN110101634A (en) * 2019-05-17 2019-08-09 亚享生物科技(江苏)有限公司 A kind of astheniz resisting balance frost and preparation method thereof
CN110812283A (en) * 2019-12-17 2020-02-21 广州市澳莱化妆品有限公司 Anti-inflammation sterilization makeup removing cream and preparation method thereof
CN111467249A (en) * 2020-04-24 2020-07-31 嘉兴力山明朗生物医药科技有限公司 Washing-free antibacterial gel and preparation method thereof

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