CN113383082A - 包含α疱疹病毒启动子序列的重组核酸 - Google Patents

包含α疱疹病毒启动子序列的重组核酸 Download PDF

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Publication number
CN113383082A
CN113383082A CN202080012381.9A CN202080012381A CN113383082A CN 113383082 A CN113383082 A CN 113383082A CN 202080012381 A CN202080012381 A CN 202080012381A CN 113383082 A CN113383082 A CN 113383082A
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seq
nucleic acid
cells
cell
promoter
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Pending
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CN202080012381.9A
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English (en)
Chinese (zh)
Inventor
E·恩格尔
C·马图拉纳
L·恩奎斯特
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Princeton University
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Princeton University
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Publication of CN113383082A publication Critical patent/CN113383082A/zh
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A61K48/0058—Nucleic acids adapted for tissue specific expression, e.g. having tissue specific promoters as part of a contruct
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16711—Varicellovirus, e.g. human herpesvirus 3, Varicella Zoster, pseudorabies
    • C12N2710/16722—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
CN202080012381.9A 2019-02-05 2020-02-05 包含α疱疹病毒启动子序列的重组核酸 Pending CN113383082A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962801524P 2019-02-05 2019-02-05
US62/801,524 2019-02-05
US201962950848P 2019-12-19 2019-12-19
US62/950,848 2019-12-19
PCT/US2020/016787 WO2020163475A1 (en) 2019-02-05 2020-02-05 Recombinant nucleic acids containing alphaherpesvirus promoter sequences

Publications (1)

Publication Number Publication Date
CN113383082A true CN113383082A (zh) 2021-09-10

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Family Applications (1)

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CN202080012381.9A Pending CN113383082A (zh) 2019-02-05 2020-02-05 包含α疱疹病毒启动子序列的重组核酸

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Country Link
US (1) US12551574B2 (enExample)
EP (1) EP3921431A4 (enExample)
JP (2) JP7658580B2 (enExample)
KR (1) KR20210125014A (enExample)
CN (1) CN113383082A (enExample)
AU (1) AU2020218337A1 (enExample)
BR (1) BR112021015281A2 (enExample)
CA (1) CA3127019A1 (enExample)
MX (1) MX2021009161A (enExample)
SG (1) SG11202108111WA (enExample)
WO (1) WO2020163475A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7658580B2 (ja) 2019-02-05 2025-04-08 ザ、トラスティーズ オブ プリンストン ユニバーシティ アルファヘルペスウイルスプロモーター配列を含む組換え核酸
US20250025575A1 (en) * 2021-11-23 2025-01-23 The Trustees Of Princeton University Compositions and Methods for Cell-Specific Expression of Target Genes
WO2026032009A1 (en) * 2024-08-06 2026-02-12 Chinese Institute For Brain Research, Beijing (Cibr) A microglia-specific regulatory element for aav-mediated transgene manipulation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1826411A (zh) * 2003-04-02 2006-08-30 新加坡科技研究局 一种用于神经元细胞基因表达的启动子构建体
US20070154456A1 (en) * 2004-02-17 2007-07-05 University Of Florida Reserch Foundation, Inc. Insulated herpesvirus-derived gene expression cassettes for sustained and regulatable gene expression

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5352596A (en) * 1992-09-11 1994-10-04 The United States Of America As Represented By The Secretary Of Agriculture Pseudorabies virus deletion mutants involving the EPO and LLT genes
EP2058401A1 (en) 2007-10-05 2009-05-13 Genethon Widespread gene delivery to motor neurons using peripheral injection of AAV vectors
JP7090548B2 (ja) 2016-01-29 2022-06-24 ジリオン バイオテック ゲーエムベーハー Ａａｖ系条件的発現系
JP7658580B2 (ja) 2019-02-05 2025-04-08 ザ、トラスティーズ オブ プリンストン ユニバーシティ アルファヘルペスウイルスプロモーター配列を含む組換え核酸
US20250025575A1 (en) 2021-11-23 2025-01-23 The Trustees Of Princeton University Compositions and Methods for Cell-Specific Expression of Target Genes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1826411A (zh) * 2003-04-02 2006-08-30 新加坡科技研究局 一种用于神经元细胞基因表达的启动子构建体
US20070154456A1 (en) * 2004-02-17 2007-07-05 University Of Florida Reserch Foundation, Inc. Insulated herpesvirus-derived gene expression cassettes for sustained and regulatable gene expression

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHEUNG AK等: "Analysis of the latency-associated transcript/UL1-3.5 gene cluster promoter complex of pseudorabies virus", ARCH VIROL, vol. 144, no. 2, 31 December 1999 (1999-12-31), pages 381 - 391, XP093129688, DOI: 10.1007/s007050050511 *
SZPARA, M, L.等: "登录号JF797219.1", NCBI_GENBANK *
TAHARAGUCHI S等: "The latency-associated transcript promoter of pseudorabies virus directs neuron-specific expression in trigeminal ganglia of transgenic mice", JOURNAL OF GENERAL VIROLOGY, vol. 84, no. 8, pages 2015 - 2022, XP055729613, DOI: 10.1099/vir.0.19080-0 *

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Publication number Publication date
EP3921431A1 (en) 2021-12-15
CA3127019A1 (en) 2020-08-13
JP7658580B2 (ja) 2025-04-08
WO2020163475A1 (en) 2020-08-13
JP2025026584A (ja) 2025-02-21
JP2022518867A (ja) 2022-03-16
BR112021015281A2 (pt) 2021-10-05
EP3921431A4 (en) 2022-12-14
KR20210125014A (ko) 2021-10-15
MX2021009161A (es) 2021-09-10
US12551574B2 (en) 2026-02-17
SG11202108111WA (en) 2021-08-30
US20220378941A1 (en) 2022-12-01
AU2020218337A1 (en) 2021-08-26

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