CN113330111A - 病毒的制造 - Google Patents

病毒的制造 Download PDF

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Publication number
CN113330111A
CN113330111A CN201980089784.0A CN201980089784A CN113330111A CN 113330111 A CN113330111 A CN 113330111A CN 201980089784 A CN201980089784 A CN 201980089784A CN 113330111 A CN113330111 A CN 113330111A
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CN
China
Prior art keywords
virus
culture
pfu
recombinant oncolytic
bioreactor
Prior art date
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Pending
Application number
CN201980089784.0A
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English (en)
Chinese (zh)
Inventor
保罗·安德鲁·赖特
凯蒂·瓦克
张明瑞
杰基·瓦瑟尔
苏珊·妮可·霍利
奥古斯特·艾伦·伦肖
朱广宇
米歇尔·凯瑟琳·文德尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Caliwell Immunotherapy
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Western Oncolytics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Western Oncolytics Ltd filed Critical Western Oncolytics Ltd
Publication of CN113330111A publication Critical patent/CN113330111A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24151—Methods of production or purification of viral material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201980089784.0A 2018-11-21 2019-11-21 病毒的制造 Pending CN113330111A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862770577P 2018-11-21 2018-11-21
US62/770,577 2018-11-21
PCT/US2019/062643 WO2020106975A1 (en) 2018-11-21 2019-11-21 Manufacture of virus

Publications (1)

Publication Number Publication Date
CN113330111A true CN113330111A (zh) 2021-08-31

Family

ID=70774620

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980089784.0A Pending CN113330111A (zh) 2018-11-21 2019-11-21 病毒的制造

Country Status (8)

Country Link
US (1) US20210277363A1 (enExample)
EP (1) EP3884044A4 (enExample)
JP (1) JP7625519B2 (enExample)
KR (1) KR20210093965A (enExample)
CN (1) CN113330111A (enExample)
AU (1) AU2019384164A1 (enExample)
CA (1) CA3120129A1 (enExample)
WO (1) WO2020106975A1 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116529382A (zh) * 2020-10-02 2023-08-01 吉恩勒克斯公司 由贴壁细胞在生物反应器中产生病毒
JP2026519561A (ja) * 2023-05-31 2026-06-16 コーニング インコーポレイテッド 固定床バイオリアクタにおける改善される細胞回収効率のための方法

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1922308A (zh) * 2004-02-23 2007-02-28 克鲁塞尔荷兰公司 病毒纯化方法
CN101058800A (zh) * 2002-07-09 2007-10-24 巴克斯特国际有限公司 用于细胞培养的无动物蛋白质培养基
US20080286850A1 (en) * 2006-09-15 2008-11-20 Medimmune Vaccines, Inc. Mdck cell lines supporting viral growth to high titers and bioreactor process using the same
CN101466720A (zh) * 2006-06-12 2009-06-24 赛托斯生物技术公司 向rna噬菌体的病毒样颗粒内包装寡核苷酸的方法
US20140162342A1 (en) * 2011-08-05 2014-06-12 Jennerex, Inc. Methods and compositions for production of vaccina virus
US10851350B1 (en) * 2017-06-27 2020-12-01 Genelux Corporation Bioreactor production of virus from adherent cells

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL131014A0 (en) 1997-01-31 2001-01-28 Schering Plough Corp Methods for cultivating cells and propagating viruses
US8003363B2 (en) * 2007-05-14 2011-08-23 Bavarian Nordic A/S Purification of vaccinia virus- and recombinant vaccinia virus-based vaccines
AU2008250596C1 (en) 2007-05-14 2010-11-25 Bavarian Nordic A/S Purification of Vaccinia virus- and recombinant Vaccinia virus-based vaccines
KR20110074563A (ko) * 2008-09-24 2011-06-30 메디뮨 엘엘씨 바이러스의 정제 방법

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101058800A (zh) * 2002-07-09 2007-10-24 巴克斯特国际有限公司 用于细胞培养的无动物蛋白质培养基
CN1922308A (zh) * 2004-02-23 2007-02-28 克鲁塞尔荷兰公司 病毒纯化方法
CN101466720A (zh) * 2006-06-12 2009-06-24 赛托斯生物技术公司 向rna噬菌体的病毒样颗粒内包装寡核苷酸的方法
US20080286850A1 (en) * 2006-09-15 2008-11-20 Medimmune Vaccines, Inc. Mdck cell lines supporting viral growth to high titers and bioreactor process using the same
US20140162342A1 (en) * 2011-08-05 2014-06-12 Jennerex, Inc. Methods and compositions for production of vaccina virus
US10851350B1 (en) * 2017-06-27 2020-12-01 Genelux Corporation Bioreactor production of virus from adherent cells

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
"Benzonase Nuclease高活力全能核酸酶", pages 2, Retrieved from the Internet <URL:https://www.biomart.cn/news/78/79/2834461.htm> *
MAFALDA G. MOLEIRINHO等: "Clinical-grade Oncolytic Adenovirus Purification Using Polysorbate 20 as an Alternative for Cell Lysis", CURRENT GENE THERAPY, 31 December 2018 (2018-12-31), pages 366 - 374 *
ZONG SHENG GUO等: "Rapid Generation of Multiple Loci-Engineered Marker-free Poxvirus and Characterization of a Clinical-Grade Oncolytic Vaccinia Virus", MOLECULAR THERAPY: METHODS & CLINICAL DEVELOPMENT, 31 December 2017 (2017-12-31), pages 120 *

Also Published As

Publication number Publication date
JP2022508156A (ja) 2022-01-19
EP3884044A4 (en) 2022-08-31
WO2020106975A1 (en) 2020-05-28
EP3884044A1 (en) 2021-09-29
JP7625519B2 (ja) 2025-02-03
US20210277363A1 (en) 2021-09-09
KR20210093965A (ko) 2021-07-28
AU2019384164A1 (en) 2021-06-03
CA3120129A1 (en) 2020-05-28

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Application publication date: 20210831