CN113311158A - Multichannel colloidal gold immunoassay appearance - Google Patents
Multichannel colloidal gold immunoassay appearance Download PDFInfo
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- CN113311158A CN113311158A CN202110628322.0A CN202110628322A CN113311158A CN 113311158 A CN113311158 A CN 113311158A CN 202110628322 A CN202110628322 A CN 202110628322A CN 113311158 A CN113311158 A CN 113311158A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
- G01N33/587—Nanoparticles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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Abstract
The invention discloses a multi-channel colloidal gold immunoassay analyzer, which comprises a shell, a sample cell and a sample cell, wherein the shell is used for protecting internal devices; the display screen is used for displaying the detection result data; the control module is used for integrally regulating and controlling detection work; the channel is used for inserting the colloidal gold test strip; the channel plate is used for placing test strips; the clamping groove is used for limiting the test strip; the positioning switch is used for determining the position of the test strip; the temperature control device is used for heating the hatching test strip; and the displacement module and the detection module are used for identifying and detecting the test strip in an all-round way. This equipment can insert simultaneously and detect the test paper strip of a plurality of differences in proper order, and the test paper strip heats the hatching after inserting the draw-in groove, and the hatching is more convenient with the integration of detection flow, practices thrift operating time to quick locking male test paper strip position, in time detect, improves detection efficiency, shows the testing data in real time, conveniently reads fast and takes notes the testing result of a plurality of test paper strips, when the test sample cardinal number is big, effectively improves detection efficiency, practices thrift check-out time.
Description
Technical Field
The invention relates to the field of analytical instruments, in particular to a multichannel colloidal gold immunoassay instrument.
Background
The colloidal gold labeling technology is a novel immunological labeling technology which uses colloidal gold as a tracer marker to be applied to antigen and antibody and has unique advantages. At present, the colloidal gold labeling technology as a rapid diagnosis method is widely applied in the medical field, in particular to the detection fields of infectious diseases, drugs, food safety and the like. The colloidal gold sample is simple to prepare, high in detection speed and widely suitable for coarse screening of basic units.
When a plurality of colloidal gold samples were examined, the sample cardinal number is big, must have special personnel on duty, and the colloidal gold analysis appearance that has now on the market simultaneously mostly only has a passageway, once only can detect a sample, is difficult to realize the joint detection of multiple a plurality of samples, and the function is single, has reduced work efficiency to a certain extent like this, has increased staff's work burden.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a multi-channel colloidal gold immunoassay analyzer, which effectively solves the problem that the detection efficiency is low when the base number of a detection sample is large because a plurality of colloidal gold test strips for detecting samples of the same or different types cannot be jointly detected at present.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a multichannel colloidal gold immunoassay appearance, includes the casing, be provided with the display screen on the casing, be provided with control module and wireless communication device in the casing, a plurality of passageways have been seted up to the side of casing, be provided with the passageway board in the casing, be provided with the draw-in groove that corresponds with the passageway on the passageway board, control module is including setting up the positioning switch on the draw-in groove, setting temperature control device in the passageway board and setting up the displacement module on the passageway board, install detection module on the displacement module.
As a further improvement of the invention, the displacement module comprises a plurality of supports arranged on the channel plate, the supports at two sides of the clamping groove are symmetrically provided with first support shafts which are parallel to the clamping groove and are sleeved with the sliding block seats, a second support shaft is arranged between the opposite sliding block seats, a stepping motor with a primary synchronous belt pulley is arranged on the sliding block seat at one side, a linkage plate is arranged on the sliding block seat at the other side, the linkage plate comprises a lead screw nut and a secondary synchronous belt pulley, the primary synchronous belt pulley is connected with the secondary synchronous belt pulley through a synchronous belt, a lead screw stepping motor is arranged on the channel plate at one side of the linkage plate, the lead screw stepping motor is connected with the lead screw nut through a lead screw, and the detection module is arranged on the second support shaft and is linked with the synchronous belt.
As a further improvement of the invention, the stepping motor is connected with the slider seat through a slider connecting plate, and a shock pad is arranged between the stepping motor and the slider connecting plate.
As a further improvement of the present invention, a first microswitch and a second microswitch are respectively arranged on the channel plate at one side of the stepping motor relative to the slider connecting plate and the detection module.
As a further improvement of the invention, the detection module comprises a camera module and a colloidal gold scanning device.
As a further improvement of the invention, the clamping groove is provided with a test paper strip fastening part.
As a further improvement of the invention, a fixed support plate is arranged on the slider seat.
As a further improvement of the invention, a printer and a USB interface are arranged on the casing.
As a further improvement of the invention, the number of the channels is 8.
The invention has the advantages that when the colloidal gold labeling technology is applied to the immune labeling test sample detection of the antigen antibody, the instrument can be simultaneously inserted with a plurality of colloidal gold test strips for detecting different or same types of samples and sequentially detects the samples, the colloidal gold test strips are heated and incubated after being inserted into the clamping grooves, the external heating and incubation are not needed, compared with the original instrument with only the detection function or the heating and incubation function, the flow is more convenient to integrate, when one test strip is detected, other test strips are in the heating and incubation state, the operation time is saved to a certain extent, the inserted test strip position can be quickly locked by using the positioning switch, the detection is carried out in time, the detection efficiency is improved, after the detection is finished, the data is displayed on the display screen in real time, and the detection results of a plurality of colloidal gold test strips can be conveniently and quickly read and recorded by workers, when the detection sample cardinality is large, the detection efficiency is effectively improved, and the detection time is saved.
Drawings
FIG. 1 is a schematic view of the external structure of the present invention;
FIG. 2 is a schematic view of the internal structure of the present invention;
FIG. 3 is a schematic view of the structure of the present invention for removing the casing and fixing the supporting plate;
FIG. 4 is a schematic view of a displacement module according to the present invention;
FIG. 5 is a schematic structural diagram of a detection module according to the present invention.
Reference numerals: 1. a housing; 11. a printer; 12. a USB interface; 2. a display screen; 21. a wireless communication device; 3. a control module; 31. a positioning switch; 32. a temperature control device; 33. a displacement module; 331. a support; 332. a first support shaft; 333. a slider seat; 3331. fixing the support plate; 3332. a slider connecting plate; 3333. a shock pad; 334. a second support shaft; 335. a stepping motor; 3351. a primary timing pulley; 336. a linkage plate; 3361. a feed screw nut; 3362. a secondary timing pulley; 337. a synchronous belt; 338. a screw rod stepping motor; 34. a detection module; 341. a camera module; 342. a colloidal gold scanning device; 4. a channel; 5. a channel plate; 51. a first microswitch; 52. a second microswitch; 6. a card slot; 7. the test paper strip fastening part.
Detailed Description
The invention will be further described in detail with reference to the following examples, which are given in the accompanying drawings.
Referring to fig. 1-5, the multichannel gold immunoassay analyzer of the present embodiment includes a housing 1, a display screen 2 is disposed on the housing 1, the display screen 2 is a touch screen, a control module 3 and a wireless communication device 21 are disposed in the housing 1, the wireless communication device 21 can perform remote wireless communication with a cloud server, spectral curve information of gold in different batches is stored in the cloud server, a plurality of channels 4 are disposed on a side surface of the housing 1, a channel plate 5 is disposed in the housing 1, a card slot 6 corresponding to the channel 4 is disposed on the channel plate 5, the control module 3 includes a positioning switch 31 disposed on the card slot 6, a temperature control device 32 disposed in the channel plate 5, and a displacement module 33 disposed on the channel plate 5, and a detection module 34 is mounted on the displacement module 33, the positioning switch 31 is a microswitch, and the temperature control device 32 is composed of a temperature measuring probe and a heating plate.
Through the technical scheme, in the multi-channel colloidal gold immunoassay analyzer of the embodiment, the control module 3 marks each card slot 6 through the positioning switch 31, and performs corresponding partition display management on the display screen 2 according to the number of the card slot 6, when the colloidal gold test strip is inserted into the card slot 6 through the channel 4 and collides with the card slot 6 to close the positioning switch 31, the control module 3 receives a signal of the positioning switch 31, locks the number of the switch, moves the displacement module 33 and the detection module 34 to detect the colloidal gold test strip in the card slot 6 where the number is located, different batches of colloidal gold spectral curve information are stored in the cloud server, the control module 3 can call the spectral curve information by using the wireless communication device 21, calibrates and checks the detected absorbance data of the colloidal gold and the called spectral curve information to determine the components and the concentration value of a detection sample, and after the checking is finished, the switch number information is sent to the display screen 2 together, the number is displayed in an area, and a worker can record the detection data information corresponding to the colloidal gold test strip in the 6-bit slot conveniently. The colloidal gold spectral curve information stored in the cloud server can be added subsequently. The temperature control device 32 in the channel plate 5, namely the temperature measuring probe and the heating plate, effectively controls the temperature of the clamping groove 6, controls the temperature in a proper temperature range for heating and hatching the test strip, and ensures normal hatching of the test strip, thereby ensuring the accuracy of detection data. The display screen 2 is a touch screen 2, after normal starting, the main interface of the display screen 2 can display touch options such as start detection, data management, parameter setting, communication test and the like, the start detection option can be clicked to enter the detection interface, the data management option can be clicked to enter a database to perform data operations such as data query, data deletion, data uploading and the like, the parameter setting option can be clicked to set parameters such as detection temperature, detection time and detection personnel, and the communication test option can be clicked to detect whether instrument communication is normal.
As an improved specific embodiment, the displacement module 33 includes a plurality of brackets 331 disposed on the channel plate 5, four of the brackets 331 are respectively disposed at four corners of the channel plate 5, the brackets 331 at two sides of the card slot 6 are symmetrically disposed with first support shafts 332 parallel to the card slot 6 and sleeved with slider holders 333, two second support shafts 334 are disposed between the opposing slider holders 333, a step motor 335 with a primary synchronous pulley 3351 is disposed on one side of the slider holder 333, a linkage plate 336 is disposed on the other side of the slider holder 333, the linkage plate 336 includes a lead screw nut 3361 and a secondary synchronous pulley 3362, the primary synchronous pulley 3351 and the secondary synchronous pulley 3362 are connected through a synchronous belt 337, a step lead screw motor 338 is disposed on the channel plate 5 at one side of the linkage plate 336, the lead screw step motor 338 and the lead screw nut 3361 are connected through a lead screw, detection module 34 installs on second support shaft 334 and links with hold-in range 337, detection module 34 and second support shaft 334 sliding connection, set up the opening that two parts passed respectively about can supplying hold-in range 337 on the detection module 34, wherein supply opening and hold-in range 337 fixed connection that hold-in range 337 lower part passed to this realizes detection module 34 and hold-in range 337 linkage.
Through the above technical solution, the brackets 331 disposed at the four corners of the channel plate 5 serve as base points for constructing the whole displacement module 33; the first supporting shafts 332 are respectively sleeved with a slider seat 333 capable of sliding in a reciprocating manner, the slider seats 333 are provided with a second supporting shaft 334 for the sliding of the detection module 34, the first supporting shafts 332 can be used for driving the slider seats 333 to control the detection module 34 to move back and forth along the insertion direction of the card slot 6, and the second supporting shafts 334 can be used for the detection module 34 to move in a reciprocating manner along the direction perpendicular to the card slot 6; the arrangement of the stepping motor 335 with the primary synchronous pulley 3351, the linkage plate 336 with the lead screw nut 3361 and the secondary synchronous pulley 3362, the lead screw stepping motor 338, the synchronous belt 337 and the through opening on the detection module 34 can directly drive the linkage plate 336 and the slider seat 333 to reciprocate on the first support shaft 332 through the operation of the lead screw stepping motor 338, and indirectly drive the detection module 34 and the stepping motor 335 to reciprocate along the insertion direction of the card slot 6; meanwhile, the timing belt 337 can be driven to rotate by the operation of the stepping motor 335, and the detection module 34 can be driven to reciprocate on the second support shaft 334 due to the linkage between the detection module 34 and the timing belt 337. Therefore, the displacement module 33 can be used to realize the omnidirectional detection of the detection module 34 in the upper plane space parallel to the channel plate 5.
As a modified specific embodiment, the stepping motor 335 is connected to the slider seat 333 through a slider connecting plate 3332, and a shock absorbing pad 3333 is disposed between the stepping motor 335 and the slider connecting plate 3332.
Through the technical scheme, the stepping motor 335 is connected with the sliding block seat 333 through the sliding block connecting plate 3332, the shock absorption pad 3333 is arranged between the stepping motor 335 and the sliding block connecting plate 3332, and the shock absorption pad can reduce the shock of the stepping motor 335 when the sliding block seat 333 drives the stepping motor 335 to reciprocate along the direction of the first supporting shaft 332, so that the dislocation of the synchronous belt 337 and the main synchronous belt wheel 3351 on the stepping motor 335 is prevented, and the subsequent normal operation of the stepping motor 335 is influenced.
As a modified specific embodiment, the channel plate 5 on the side of the stepping motor 335 is provided with a first microswitch 51 and a second microswitch 52 with respect to the slider connecting plate 3332 and the detection module 34, respectively.
Through the above technical solution, the position of the first micro switch 51 is used as the slider connection plate 3332, and is also the initial position of the detection module 34 moving along the direction of the first support shaft 332, when the slider connection plate touches the first micro switch 51, the control module 3 can know that the detection module 34 has returned to the initial position moving along the direction of the first support shaft 332; the position of the second microswitch 52 is used as the initial position of the detection module 34 moving along the direction of the second support shaft 334, when the detection module 34 touches the second microswitch 52, the control module 3 can know that the detection module 34 has returned to the initial position moving along the direction of the second support shaft 334, the two microswitches are used for ensuring that the detection module 34 successfully returns to the initial position after the detection work is completed, and the situation that the detection module stops at other positions to cause false detection or re-detection is avoided.
As a modified specific embodiment, the detection module 34 includes a camera module 341 and a colloidal gold scanning device 342, the camera module 341 employs a camera capable of scanning a two-dimensional code, two-dimensional codes of different batches of information are marked on a colloidal gold test strip, the different two-dimensional codes are identified by using the camera on the detection module 34, and the colloidal gold scanning device 342 is an optical module having a transmitter and a receiver inside.
Through above-mentioned technical scheme, mark the two-dimensional code of different grade type batch on the colloidal gold test paper strip to being recorded and storing, the sample of different grade type is detected in the two-dimensional code representative of different grade type batch, can realize detecting the colloidal gold test paper strip of a plurality of different or same type samples in same platform analysis appearance, promote detection efficiency. The camera module 341 on the detection module 34 employs a camera capable of scanning the two-dimensional code to scan and read the two-dimensional code detection information marked on the colloidal gold test strip, and after the type batch information is read, the two-dimensional code detection information can be timely reflected to the control module 3, and the control module 3 performs relevant regulation and control on the temperature, so as to ensure the correctness and accuracy of the detection result. Colloidal gold immunoassay appearance is under operating condition, casing 1 is inside to be in a dark space, colloidal gold scanning device 342 is that the optical module utilizes the transmitter to the colloidal gold emission light on the colloidal gold test paper strip, utilize the receiver to receive the light after the reflection of colloidal gold test paper strip simultaneously, the light of contrast outgoing and the light of receiving back, thereby reach the absorbance of colloidal gold test paper strip, do a relevant record to it, and carry out the conversion of analog quantity and digital quantity in detection module 34, follow-up transmit data for control module 3, control module 3 calibrates data and colloidal gold spectral curve information and checks, and show final testing result on display screen 2.
As an improved specific embodiment, the card slot 6 is provided with a test strip fastening part 7, and the test strip fastening part 7 includes an extension strip and a collision spring piece for limiting the colloidal gold test strip.
Through above-mentioned technical scheme, colloidal gold test paper strip passes through passageway 4, inserts draw-in groove 6 back, is earlier by the scope that the epitaxial strip restriction reciprocated, when the test paper strip was contradicted the shell fragment, contradicts the shell fragment with test paper strip jack-up, makes it hug closely the epitaxial strip, can't reciprocate, can only realize inserting and extract the operation to play the fastening effect.
As a modified specific embodiment, the slider seat 333 is provided with a fixed support plate 3331, and the fixed support plate 3331 is located above the slider seat 333 and is fixedly connected with all the slider seats 333.
Through the technical scheme, the fixed support plate 3331 is arranged on the sliding block seat 333, so that all the sliding block seats 333 can move in the same direction at the same time, and the phenomenon that any one sliding block seat 333 is not coordinated with other sliding block seats 333 and normal operation of an instrument is influenced is avoided. And the arrangement of the fixed support plate 3331 may also provide a certain degree of protection for the detection module 34.
As a modified specific embodiment, the casing 1 is provided with a printer 11 and a USB interface 12.
Through the technical scheme, after the detection and data check analysis of the colloidal gold test strip are completed, the detection data information can be printed by the printer 11 to be recorded on paper, so that the experimental data can be conveniently stored. The USB interface 12 can be externally connected with a mobile data storage device, and an electronic file is reserved for the detection result, so that the situations that the detection result is lost, the detection needs to be carried out again and the like are avoided; the USB interface 12 can be used for connecting a mobile data storage device externally, and colloidal gold spectral curve information stored in the mobile data storage device is used for helping to complete the detection process.
As a modified specific embodiment, the number of the passages 4 is 8.
Through the technical scheme, the number of the detected channels 4 is set to 8, so that the detection efficiency can be improved to a certain extent, the display partitions of the display screen 2 are set according to the number of the channels 4, the display area of the display screen is limited, the 8 display partitions can present clear data information with reasonable font size, and the display resources of the display screen 2 cannot be wasted.
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that modifications and embellishments within the scope of the invention may occur to those skilled in the art without departing from the principle of the invention, and are considered to be within the scope of the invention.
Claims (9)
1. A multichannel colloidal gold immunoassay appearance which characterized in that: including casing (1), be provided with display screen (2) on casing (1), be provided with control module (3) and wireless communication device (21) in casing (1), a plurality of passageways (4) have been seted up to the side of casing (1), be provided with passageway board (5) in casing (1), be provided with draw-in groove (6) that correspond with passageway (4) on passageway board (5), control module (3) are including setting up positioning switch (31) on draw-in groove (6), setting up temperature control device (32) in passageway board (5) and setting up displacement module (33) on passageway board (5), install detection module (34) on displacement module (33).
2. The multi-channel colloidal gold immunoassay analyzer of claim 1, wherein: the displacement module (33) comprises a plurality of supports (331) arranged on a channel plate (5), the supports (331) on two sides of the clamping groove (6) are symmetrically provided with first support shafts (332) which are parallel to the clamping groove (6) and are sleeved with sliding block seats (333), a second support shaft (334) is arranged between the opposite sliding block seats (333), a stepping motor (335) with a primary synchronous pulley (3351) is arranged on the sliding block seat (333) on one side, a linkage plate (336) is arranged on the sliding block seat (333) on the other side, the linkage plate (336) comprises a lead screw nut (3361) and a secondary synchronous pulley (3362), the primary synchronous pulley (3351) is connected with the secondary synchronous pulley (3362) through a synchronous belt (337), a lead screw stepping motor (338) is arranged on the channel plate (5) on one side of the linkage plate (336), and the lead screw stepping motor (338) is connected with the lead screw nut (3361), the detection module (34) is installed on the second support shaft (334) and is linked with the synchronous belt (337).
3. The multi-channel colloidal gold immunoassay analyzer of claim 2, wherein: the stepping motor (335) is connected with the sliding block seat (333) through a sliding block connecting plate (3332), and a damping pad (3333) is arranged between the stepping motor (335) and the sliding block connecting plate (3332).
4. The multi-channel colloidal gold immunoassay analyzer of claim 3, wherein: and a first microswitch (51) and a second microswitch (52) are respectively arranged on the channel plate (5) at one side of the stepping motor (335) relative to the slider connecting plate (3332) and the detection module (34).
5. The multi-channel colloidal gold immunoassay analyzer of claim 4, wherein: the detection module (34) comprises a camera module (341) and a colloidal gold scanning device (342).
6. The multi-channel colloidal gold immunoassay analyzer of claim 5, wherein: and a test paper strip fastening part (7) is arranged on the clamping groove (6).
7. The multi-channel colloidal gold immunoassay analyzer of claim 6, wherein: and a fixed support plate (3331) is arranged on the sliding block seat (333).
8. The multi-channel colloidal gold immunoassay analyzer according to any one of claims 1 to 7, wherein: the casing (1) is provided with a printer (11) and a USB interface (12).
9. The multi-channel colloidal gold immunoassay analyzer according to any one of claims 1 to 7, wherein: the number of the channels (4) is 8.
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| CN202110628322.0A CN113311158A (en) | 2021-06-04 | 2021-06-04 | Multichannel colloidal gold immunoassay appearance |
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| CN202110628322.0A CN113311158A (en) | 2021-06-04 | 2021-06-04 | Multichannel colloidal gold immunoassay appearance |
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| CN210982464U (en) * | 2019-10-30 | 2020-07-10 | 浙江恩谱生物科技有限公司 | Fluorescence immunoassay appearance with heating device |
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2021
- 2021-06-04 CN CN202110628322.0A patent/CN113311158A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
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| GB1469172A (en) * | 1973-03-05 | 1977-03-30 | Varian Associates | Fluid transfer apparatus |
| CN106370836A (en) * | 2016-09-26 | 2017-02-01 | 佰奥达生物科技（武汉）股份有限公司 | Immune detector and use method thereof |
| CN106483283A (en) * | 2016-11-28 | 2017-03-08 | 贝知（上海）信息科技有限公司 | A kind of diagnostic test system |
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| CN110031618A (en) * | 2019-04-22 | 2019-07-19 | 广西埃韦迪生物科技有限公司 | A kind of medical ten channels colloidal gold analyzer |
| CN210982464U (en) * | 2019-10-30 | 2020-07-10 | 浙江恩谱生物科技有限公司 | Fluorescence immunoassay appearance with heating device |
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