CN113304097A - Essence containing stem cell exosomes and preparation method thereof - Google Patents
Essence containing stem cell exosomes and preparation method thereof Download PDFInfo
- Publication number
- CN113304097A CN113304097A CN202110587344.7A CN202110587344A CN113304097A CN 113304097 A CN113304097 A CN 113304097A CN 202110587344 A CN202110587344 A CN 202110587344A CN 113304097 A CN113304097 A CN 113304097A
- Authority
- CN
- China
- Prior art keywords
- parts
- stem cell
- solution
- collagen
- essence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/736—Chitin; Chitosan; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
- A61K8/985—Skin or skin outgrowth, e.g. hair, nails
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Zoology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses an essence containing stem cell exosomes, belonging to the technical field of cosmetic preparation, and comprising the following raw materials in parts by weight: 10-13 parts of skin stem cells, 15-20 parts of sodium hyaluronate, 6-8 parts of denatured ethanol, 2-6 parts of hydroxyethyl piperazine ethane sulfonic acid, 5-8 parts of modified collagen, 4-6 parts of silicone wax, 3-5 parts of octyl dodecanol, 2-4 parts of ascorbic acid glucoside, 1-3 parts of ammonium polyacryl dimethyl taurate and 10-12 parts of deionized water; the invention also discloses a preparation method of the essence. The skin stem cell exosome is used in the essence, so that the water locking capacity of the essence on the skin can be greatly improved, the skin fineness is improved, and meanwhile, the modified collagen is rich in collagen, chitosan and camellia seed extract, so that the modified collagen has high nutritional value and has the characteristics of moisture resistance, oxygen resistance, antibiosis, antioxidation and moisture retention.
Description
Technical Field
The invention belongs to the technical field of cosmetic preparation, and relates to essence containing stem cell exosomes and a preparation method thereof.
Background
The skin is the largest organ of the human body, is covered on the surface of the body, consists of epidermis, dermis, subcutaneous tissue and appendages, and plays an important role in resisting invasion of microorganisms and ultraviolet radiation, preventing water loss, regulating the body temperature, maintaining the appearance of the human body and the like. In recent years, the use of living cells for beauty treatment has become a hot spot in the market, and particularly, the use of stem cells enables the living cells to differentiate and grow in the skin through direct injection on wrinkles, scars and scars, thereby playing a filling and repairing function on the skin.
However, the beauty effect is realized by simply using the stem cell extracting solution through an injection mode, the effect is slow, a professional needs to use medical equipment in a specified place by an injection method, people who are the same are limited, and products of the essence containing the stem cell exosomes and having the effects of whitening, moisturizing and resisting oxidation are few, so that the problem can be solved by developing the essence containing the stem cell exosomes and having the effects of moisturizing, whitening, resisting oxidation.
Disclosure of Invention
The invention aims to provide essence containing stem cell exosomes and a preparation method thereof.
The problems to be solved by the invention are as follows: the beauty effect is realized by simply using the stem cell extracting solution in an injection mode, the effect is slow, a professional needs to use medical equipment in a specified place by an injection method, the people who suffer from the beauty effect are limited, and products containing the stem cell exosome essence with the effects of whitening, moisturizing and resisting oxidation are few.
The purpose of the invention can be realized by the following technical scheme:
an essence containing stem cell exosomes comprises the following raw materials in parts by weight:
10-13 parts of skin stem cells, 15-20 parts of sodium hyaluronate, 6-8 parts of denatured ethanol, 2-6 parts of hydroxyethyl piperazine ethane sulfonic acid, 5-8 parts of modified collagen, 4-6 parts of silicone wax, 3-5 parts of octyl dodecanol, 2-4 parts of ascorbic acid glucoside, 1-3 parts of ammonium polyacryl dimethyl taurate and 10-12 parts of deionized water;
the essence containing the stem cell exosomes is prepared by the following steps:
step A1, adding electrotransfection working solution and CTF1 plasmid into skin stem cells to obtain a pre-transfection product;
step A2, carrying out electric shock on the pre-transfection object to obtain skin stem cells transfected with CTF1 genes;
step A3, culturing the skin stem cells transfected with CTF1 gene, collecting and centrifuging to obtain a stem cell secretion;
step A4, adding sodium hyaluronate, modified collagen, denatured ethanol, organic silicon wax and hydroxyethyl piperazine ethanesulfonic acid into a stirring tank, and stirring and mixing at 45-50 ℃ and 250-300rpm for 30-60min to obtain a mixture A;
step A5, adding the mixture A, octyldodecanol, ascorbic acid glucoside, polyacrylamide dimethyl taurinate and deionized water into an emulsifying pot, and emulsifying at 70-80 ℃ for 10-15min to obtain a mixture B;
step A6, adding the stem cell secretion into the mixture B, stirring and mixing at 150-160rpm for 20-30min to obtain the essence containing the stem cell secretion.
Further, the dosage of the electrotransfection working solution in the step A1 is 8-10 times of the mass of the skin stem cells, and the mass of the CTF1 plasmid is 10-20% of the mass of the skin stem cells.
The modified collagen is prepared by the following steps:
step S1, weighing collagen in a beaker, adding deionized water to prepare a solution with the concentration of 20g/L, heating to 50 ℃ to react for 30-40min, and removing precipitates through suction filtration to obtain a collagen solution;
step S2, adding an acetic acid solution with the volume fraction of 2% into chitosan to prepare a solution with the volume fraction of 20g/L, heating to 50 ℃ and reacting for 30-40min to obtain a chitosan solution;
step S3, crushing the camellia seeds into camellia seed powder of 40 meshes, adding petroleum ether into the camellia seed powder, stirring and reacting for 20-24h at room temperature, standing for 6h, performing suction filtration, taking supernatant, performing rotary evaporation and concentration to one third of the original volume to obtain a camellia seed extract;
step S4, mixing the collagen solution and the chitosan solution according to the ratio of 1: 4.2-4.5, adding camellia seed extract and glycerol with the mass fraction of 25%, stirring for 10min at 20 ℃, and heating to 60 ℃ for reflux reaction for 1-2h to obtain the modified collagen.
Further, in the step S3, the mass ratio of the camellia seed powder to the petroleum ether is 1: 10.
further, in step S4, the amount of the camellia seed extract is 5-10% of the total mass of the collagen solution and the chitosan solution, and the amount of the glycerol is 3-5% of the total mass of the collagen solution and the chitosan solution.
A preparation method of essence containing stem cell exosomes comprises the following preparation steps:
step A1, adding electrotransfection working solution and CTF1 plasmid into skin stem cells to obtain a pre-transfection product;
step A2, carrying out electric shock on the pre-transfection object to obtain skin stem cells transfected with CTF1 genes;
step A3, culturing the skin stem cells transfected with CTF1 gene, collecting and centrifuging to obtain a stem cell secretion;
step A4, adding sodium hyaluronate, modified collagen, denatured ethanol, organic silicon wax and hydroxyethyl piperazine ethanesulfonic acid into a stirring tank, and stirring and mixing at 45-50 ℃ and 250-300rpm for 30-60min to obtain a mixture A;
step A5, adding the mixture A, octyldodecanol, ascorbic acid glucoside, polyacrylamide dimethyl taurinate and deionized water into an emulsifying pot, and emulsifying at 70-80 ℃ for 10-15min to obtain a mixture B;
step A6, adding the stem cell secretion into the mixture B, stirring and mixing at 150-160rpm for 20-30min to obtain the essence containing the stem cell secretion.
The invention has the beneficial effects that: the invention aims to provide essence containing stem cell exosomes and a preparation method thereof, the skin stem cell exosomes are used in the essence, the water-locking capacity of the essence on skin can be greatly improved, the skin fineness is improved, meanwhile, modified collagen is rich in collagen, chitosan and an oil tea seed extract, the essence has high nutritive value, has the characteristics of moisture resistance, oxygen resistance, antibiosis, antioxidation and moisture retention, can prevent oxygen from entering, inhibit the oxidation of active ingredients and the breeding of microorganisms, and has the effects of water replenishing and nourishing, oil tea seeds contain a large amount of flavone, polysaccharide, tea polyphenol and other substances, and the substances have the effects of oxidation resistance and aging resistance, are beneficial to the repair and the maintenance of the skin, and have the effects of high-efficiency water replenishing, whitening and spot lightening by matching with the stem cell exosomes.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The modified collagen is prepared by the following steps:
step S1, weighing collagen in a beaker, adding deionized water to prepare a solution with the concentration of 20g/L, heating to 50 ℃ to react for 30min, and removing precipitates through suction filtration to obtain a collagen solution;
step S2, adding an acetic acid solution with the volume fraction of 2% into chitosan to prepare a solution with the volume fraction of 20g/L, heating to 50 ℃ and reacting for 30min to obtain a chitosan solution;
step S3, crushing the camellia seeds into camellia seed powder of 40 meshes, adding petroleum ether into the camellia seed powder, stirring and reacting for 20 hours at room temperature, standing for 6 hours, performing suction filtration, taking supernatant, performing rotary evaporation and concentration to one third of the original volume to obtain a camellia seed extract, wherein the mass ratio of the camellia seed powder to the petroleum ether is 1: 10;
step S4, mixing the collagen solution and the chitosan solution according to the ratio of 1: 4.2, adding the camellia seed extract and 25 mass percent of glycerol, stirring for 10min at 20 ℃, heating to 60 ℃, and performing reflux reaction for 1h to obtain the modified collagen, wherein the dosage of the camellia seed extract is 5 percent of the total mass of the collagen solution and the chitosan solution, and the dosage of the glycerol is 3 percent of the total mass of the collagen solution and the chitosan solution.
Example 2
The modified collagen is prepared by the following steps:
step S1, weighing collagen in a beaker, adding deionized water to prepare a solution with the concentration of 20g/L, heating to 50 ℃ to react for 30min, and removing precipitates through suction filtration to obtain a collagen solution;
step S2, adding an acetic acid solution with the volume fraction of 2% into chitosan to prepare a solution with the volume fraction of 20g/L, heating to 50 ℃ and reacting for 30min to obtain a chitosan solution;
step S3, crushing the camellia seeds into camellia seed powder of 40 meshes, adding petroleum ether into the camellia seed powder, stirring and reacting for 20 hours at room temperature, standing for 6 hours, performing suction filtration, taking supernatant, performing rotary evaporation and concentration to one third of the original volume to obtain a camellia seed extract, wherein the mass ratio of the camellia seed powder to the petroleum ether is 1: 10;
step S4, mixing the collagen solution and the chitosan solution according to the ratio of 1: 4.3, adding the camellia seed extract and 25 mass percent of glycerol, stirring for 10min at 20 ℃, heating to 60 ℃, and performing reflux reaction for 1h to obtain the modified collagen, wherein the dosage of the camellia seed extract is 7 percent of the total mass of the collagen solution and the chitosan solution, and the dosage of the glycerol is 4 percent of the total mass of the collagen solution and the chitosan solution.
Example 3
The modified collagen is prepared by the following steps:
step S1, weighing collagen in a beaker, adding deionized water to prepare a solution with the concentration of 20g/L, heating to 50 ℃ to react for 40min, and removing precipitates through suction filtration to obtain a collagen solution;
step S2, adding an acetic acid solution with the volume fraction of 2% into chitosan to prepare a solution with the volume fraction of 20g/L, heating to 50 ℃ and reacting for 40min to obtain a chitosan solution;
step S3, crushing the camellia seeds into camellia seed powder of 40 meshes, adding petroleum ether into the camellia seed powder, stirring and reacting for 24 hours at room temperature, standing for 6 hours, performing suction filtration, taking supernatant, performing rotary evaporation and concentration to one third of the original volume to obtain a camellia seed extract, wherein the mass ratio of the camellia seed powder to the petroleum ether is 1: 10;
step S4, mixing the collagen solution and the chitosan solution according to the ratio of 1: and 4.5, uniformly mixing, adding the camellia seed extract and 25 mass percent of glycerol, stirring for 10min at 20 ℃, heating to 60 ℃, and carrying out reflux reaction for 2h to obtain the modified collagen, wherein the dosage of the camellia seed extract is 10 percent of the total mass of the collagen solution and the chitosan solution, and the dosage of the glycerol is 5 percent of the total mass of the collagen solution and the chitosan solution.
Example 4
An essence containing stem cell exosomes comprises the following raw materials in parts by weight:
10 parts of skin stem cells, 15 parts of sodium hyaluronate, 6 parts of denatured ethanol, 2 parts of hydroxyethyl piperazine ethane sulfonic acid, 5 parts of modified collagen, 4 parts of silicone wax, 3 parts of octyl dodecanol, 2 parts of ascorbyl glucoside, 1 part of ammonium polyacryl dimethyl taurate and 10 parts of deionized water;
the essence containing the stem cell exosomes is prepared by the following steps:
step A1, adding electrotransfection working solution and CTF1 plasmid into skin stem cells to obtain a pre-transfection product, wherein the using amount of the electrotransfection working solution is 8 times of the mass of the skin stem cells, and the CTF1 plasmid is 10% of the mass of the skin stem cells;
step A2, carrying out electric shock on the pre-transfection object to obtain skin stem cells transfected with CTF1 genes;
step A3, culturing the skin stem cells transfected with CTF1 gene, collecting and centrifuging to obtain a stem cell secretion;
step A4, adding sodium hyaluronate, the modified collagen prepared in example 1, denatured ethanol, silicone wax and hydroxyethyl piperazine ethanesulfonic acid into a stirring tank, and stirring and mixing at 45 ℃ and 250rpm for 30min to obtain a mixture A;
step A5, adding the mixture A, octyldodecanol, ascorbyl glucoside, polyacrylamide dimethyl taurate and deionized water into an emulsifying pot, and emulsifying at 70 ℃ for 10min to obtain a mixture B;
and step A6, adding the stem cell secretion into the mixture B, stirring and mixing at 150rpm for 20min to obtain essence containing the stem cell secretion.
Example 5
An essence containing stem cell exosomes comprises the following raw materials in parts by weight:
12 parts of skin stem cells, 17 parts of sodium hyaluronate, 7 parts of denatured ethanol, 4 parts of hydroxyethyl piperazine ethane sulfonic acid, 6 parts of modified collagen, 5 parts of silicone wax, 4 parts of octyl dodecanol, 3 parts of ascorbyl glucoside, 2 parts of ammonium polyacryl dimethyl taurate and 11 parts of deionized water;
the essence containing the stem cell exosomes is prepared by the following steps:
step A1, adding electrotransfection working solution and CTF1 plasmid into skin stem cells to obtain a pre-transfection product, wherein the using amount of the electrotransfection working solution is 9 times of the mass of the skin stem cells, and the CTF1 plasmid is 15% of the mass of the skin stem cells;
step A2, carrying out electric shock on the pre-transfection object to obtain skin stem cells transfected with CTF1 genes;
step A3, culturing the skin stem cells transfected with CTF1 gene, collecting and centrifuging to obtain a stem cell secretion;
step A4, adding sodium hyaluronate, the modified collagen prepared in example 2, denatured ethanol, silicone wax and hydroxyethyl piperazine ethanesulfonic acid into a stirring tank, and stirring and mixing at 45 ℃ and 250rpm for 30min to obtain a mixture A;
step A5, adding the mixture A, octyldodecanol, ascorbyl glucoside, polyacrylamide dimethyl taurate and deionized water into an emulsifying pot, and emulsifying at 70 ℃ for 10min to obtain a mixture B;
and step A6, adding the stem cell secretion into the mixture B, stirring and mixing at 155rpm for 25min to obtain the essence containing the stem cell secretion.
Example 6
An essence containing stem cell exosomes comprises the following raw materials in parts by weight:
13 parts of skin stem cells, 20 parts of sodium hyaluronate, 8 parts of denatured ethanol, 6 parts of hydroxyethyl piperazine ethane sulfonic acid, 8 parts of modified collagen, 6 parts of silicone wax, 5 parts of octyl dodecanol, 4 parts of ascorbyl glucoside, 3 parts of ammonium polyacryl dimethyl taurate and 12 parts of deionized water;
the essence containing the stem cell exosomes is prepared by the following steps:
step A1, adding electrotransfection working solution and CTF1 plasmid into skin stem cells to obtain a pre-transfection product, wherein the using amount of the electrotransfection working solution is 10 times of the mass of the skin stem cells, and the CTF1 plasmid is 20 percent of the mass of the skin stem cells;
step A2, carrying out electric shock on the pre-transfection object to obtain skin stem cells transfected with CTF1 genes;
step A3, culturing the skin stem cells transfected with CTF1 gene, collecting and centrifuging to obtain a stem cell secretion;
step A4, adding sodium hyaluronate, the modified collagen prepared in example 3, denatured ethanol, silicone wax and hydroxyethyl piperazine ethanesulfonic acid into a stirring tank, and stirring and mixing at 50 ℃ and 300rpm for 60min to obtain a mixture A;
step A5, adding the mixture A, octyldodecanol, ascorbyl glucoside, polyacrylamide dimethyl taurate and deionized water into an emulsifying pot, and emulsifying at 80 ℃ for 15min to obtain a mixture B;
and step A6, adding the stem cell secretion into the mixture B, stirring and mixing at 160rpm for 30min to prepare the essence containing the stem cell secretion.
The essence prepared in examples 4-6 was tested for efficacy, 120 female subjects with significant wrinkles and color spots on their faces over 40 years old were selected and evenly divided into 3 groups, and the essence was applied every morning and evening to observe the efficacy, with the test data shown in table 1:
TABLE 1
As can be seen from table 1, the essence containing the stem cell exosomes prepared in examples 4 to 6 has excellent effects of repairing wrinkles, lightening spots, and moisturizing and whitening.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (5)
1. The essence containing the stem cell exosomes is characterized by comprising the following raw materials in parts by weight: 10-13 parts of skin stem cells, 15-20 parts of sodium hyaluronate, 6-8 parts of denatured ethanol, 2-6 parts of hydroxyethyl piperazine ethane sulfonic acid, 5-8 parts of modified collagen, 4-6 parts of silicone wax, 3-5 parts of octyl dodecanol, 2-4 parts of ascorbic acid glucoside, 1-3 parts of ammonium polyacryl dimethyl taurate and 10-12 parts of deionized water;
the modified collagen is prepared by the following steps:
step S1, adding deionized water into collagen to prepare a solution of 20g/L, heating to 50 ℃ to react for 30-40min, and removing precipitates by suction filtration to obtain a collagen solution;
step S2, adding acetic acid solution into chitosan to prepare 20g/L solution, heating to 50 ℃ and reacting for 30-40min to obtain chitosan solution;
step S3, crushing the camellia seeds into camellia seed powder of 40 meshes, adding petroleum ether into the camellia seed powder, stirring and reacting for 20-24h at room temperature, standing, performing suction filtration, taking supernatant, performing rotary evaporation and concentration to one third of the original volume to obtain a camellia seed extract;
step S4, mixing the collagen solution and the chitosan solution according to the ratio of 1: 4.2-4.5, adding camellia seed extract and glycerol with the mass fraction of 25%, stirring for 10min at 20 ℃, and heating to 60 ℃ for reflux reaction for 1-2h to obtain the modified collagen.
2. The essence containing stem cell exosomes according to claim 1, characterized in that: in the step S3, the mass ratio of the camellia seed powder to the petroleum ether is 1: 10.
3. the essence containing stem cell exosomes according to claim 1, characterized in that: in the step S4, the dosage of the camellia seed extract is 5-10% of the total mass of the collagen solution and the chitosan solution, and the dosage of the glycerol is 3-5% of the total mass of the collagen solution and the chitosan solution.
4. The method for preparing essence containing stem cell exosomes according to claim 1, wherein the essence comprises the following steps: the preparation method comprises the following preparation processes:
step A1, adding electrotransfection working solution and CTF1 plasmid into skin stem cells to obtain a pre-transfection product;
step A2, carrying out electric shock on the pre-transfection object to obtain skin stem cells transfected with CTF1 genes;
step A3, culturing the skin stem cells transfected with CTF1 gene, collecting and centrifuging to obtain a stem cell secretion;
step A4, uniformly stirring sodium hyaluronate, modified collagen, denatured ethanol, organic silicon wax and hydroxyethyl piperazine ethanesulfonic acid to obtain a mixture A;
step A5, uniformly mixing the mixture A, octyldodecanol, ascorbyl glucoside, ammonium polyacryloyl dimethyl taurate and deionized water, and emulsifying at 70-80 ℃ for 10-15min to obtain a mixture B;
and step A6, adding the stem cell secretion into the mixture B, and uniformly stirring to obtain the essence containing the stem cell secretion.
5. The method for preparing essence containing stem cell exosomes according to claim 4, wherein the essence comprises the following steps: the dosage of the electrotransfection working solution in the step A1 is 8-10 times of the mass of the skin stem cells, and the mass of the CTF1 plasmid is 10-20% of the mass of the skin stem cells.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110587344.7A CN113304097A (en) | 2021-05-27 | 2021-05-27 | Essence containing stem cell exosomes and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110587344.7A CN113304097A (en) | 2021-05-27 | 2021-05-27 | Essence containing stem cell exosomes and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113304097A true CN113304097A (en) | 2021-08-27 |
Family
ID=77375789
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110587344.7A Pending CN113304097A (en) | 2021-05-27 | 2021-05-27 | Essence containing stem cell exosomes and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113304097A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109172404A (en) * | 2018-10-18 | 2019-01-11 | 周君梅 | A kind of anti-wrinkle essence and preparation method thereof |
| CN111437244A (en) * | 2020-04-15 | 2020-07-24 | 北京本真工坊生物科技有限公司 | Preparation method and product of high-efficiency stem cell expression exosome for skin beauty |
| CN111643527A (en) * | 2020-07-22 | 2020-09-11 | 北京岳昊科技发展有限公司 | Application of transgenic stem cell exosome in preparation of medicine or whitening cosmetics |
| CN112641714A (en) * | 2021-01-07 | 2021-04-13 | 上海南滨江细胞生物科技有限公司 | Preparation method of essence containing autologous adipose-derived stem cell cytokines |
| CN112662630A (en) * | 2021-02-01 | 2021-04-16 | 菲尔生物工程技术有限公司 | Application of stem cell exosome in preparation of essence |
-
2021
- 2021-05-27 CN CN202110587344.7A patent/CN113304097A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109172404A (en) * | 2018-10-18 | 2019-01-11 | 周君梅 | A kind of anti-wrinkle essence and preparation method thereof |
| CN111437244A (en) * | 2020-04-15 | 2020-07-24 | 北京本真工坊生物科技有限公司 | Preparation method and product of high-efficiency stem cell expression exosome for skin beauty |
| CN111643527A (en) * | 2020-07-22 | 2020-09-11 | 北京岳昊科技发展有限公司 | Application of transgenic stem cell exosome in preparation of medicine or whitening cosmetics |
| CN112641714A (en) * | 2021-01-07 | 2021-04-13 | 上海南滨江细胞生物科技有限公司 | Preparation method of essence containing autologous adipose-derived stem cell cytokines |
| CN112662630A (en) * | 2021-02-01 | 2021-04-16 | 菲尔生物工程技术有限公司 | Application of stem cell exosome in preparation of essence |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108670943B (en) | Plant extract composition with moisturizing, whitening and spot-lightening functions | |
| CN105434239B (en) | A kind of application of skin lightening compositions containing natural botanical source in cosmetics | |
| CN107137318A (en) | A kind of ferment facial mask for improving skin moisture function and preparation method thereof | |
| CN107693456A (en) | A kind of natural tea pasta frost and preparation method thereof | |
| KR101606393B1 (en) | Cosmetic composition containing scoria | |
| CN102209527B (en) | Antioxidant cosmetic composition containing extract of processed peony, polygonati rhizoma or lily | |
| CN113576975A (en) | Easily-absorbed anti-aging composition and preparation method and application thereof | |
| CN108670933B (en) | Skin care composition with moisturizing, whitening and anti-aging functions and application thereof | |
| CN110302128A (en) | A kind of whitening antiallergic rich in natural product extraction ingredient is releived sleep mask and preparation method thereof | |
| KR20130051543A (en) | Manufacturing method of composition of cosmetic using plant extract from codonopsis lanceolata and the composition of cosmetic | |
| CN113304097A (en) | Essence containing stem cell exosomes and preparation method thereof | |
| CN111643441A (en) | Antioxidant whitening moisturizing liquid containing stem cell extracting solution and preparation method thereof | |
| CN109646329A (en) | It is a kind of to drop the essential oil content in freckle cream and preparation method thereof that smells good | |
| KR102386118B1 (en) | Composition comprising Lespedeza plant extracts for skin volume augmentation | |
| CN114344211A (en) | Moisturizing and stabilizing composition and preparation method thereof | |
| CN113081924A (en) | Antioxidant composition, preparation method and application | |
| CN108619020B (en) | A facial mask containing herba Ixeritis Sonchifoliae extract and its preparation method | |
| CN112870141A (en) | Edible natural plant skin-care mask lotion and preparation method thereof | |
| CN112618441A (en) | Anti-allergy whitening moisturizing composition and preparation method thereof | |
| CN112656739A (en) | Shampoo containing natural plants and preparation method thereof | |
| CN113143841B (en) | Whitening and moisturizing gel mask containing calendula officinalis extract as well as preparation method and application thereof | |
| KR101831756B1 (en) | Cosmetic composition for improving wrinkles and the patch for the skin around eyes using the same | |
| CN110585107A (en) | Wonderful age essence capable of firming skin, moistening skin, beautifying skin and deeply replenishing water and preparation method thereof | |
| KR100983326B1 (en) | Cosmetic composition containing fermented media of achyranthes japonica using mineral water to be electrolysis | |
| CN112972276A (en) | Moisturizing and hydrating face cream and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210827 |
|
| RJ01 | Rejection of invention patent application after publication |