CN112662630A - Application of stem cell exosome in preparation of essence - Google Patents
Application of stem cell exosome in preparation of essence Download PDFInfo
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- CN112662630A CN112662630A CN202110135477.0A CN202110135477A CN112662630A CN 112662630 A CN112662630 A CN 112662630A CN 202110135477 A CN202110135477 A CN 202110135477A CN 112662630 A CN112662630 A CN 112662630A
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Abstract
The invention discloses an application of a stem cell exosome in the aspect of preparing essence, wherein the stem cell exosome is a skin stem cell exosome, and a preparation method of the stem cell exosome comprises the following steps: taking human skin stem cells for later use; mixing the skin stem cells with the electrotransfection working solution to obtain a mixed solution, and mixing the mixed solution with CTF1 plasmid to obtain a pre-transfection product; carrying out electric shock on the pre-transfection object to obtain skin stem cells transfected with CTF1 genes; this stem cell exosome is with skin stem cell exosome for in the essence, improvement that can be very big, the essence is to the lock water ability of skin, improves the fine and smooth degree of skin, has added modified xylitol moreover, further increases the effect, it is used for preparing the essence to have solved skin stem cell exosome, the maintenance of improvement skin moisture that can be very big, the fine and smooth degree of improvement skin, but present essence does not use stem cell exosome, can't satisfy the problem of the demand of people to the lock water ability of skin.
Description
Technical Field
The invention relates to essence, in particular to application of stem cell exosomes in preparation of the essence.
Background
The skin, the largest organ of the human body, is covered on the surface of the body and consists of the epidermis, the dermis, the subcutaneous tissue and the appendages. Plays an important role in resisting microorganism invasion and ultraviolet radiation, preventing water loss, regulating body temperature, maintaining human appearance and the like.
The skin stem cell exosome is used for preparing essence, can greatly improve the moisture retention of skin and improve the skin fineness, but the stem cell exosome is not used in the existing essence, and the requirement of people on the water locking capacity of skin cannot be met.
Disclosure of Invention
The invention aims to provide application of stem cell exosomes in preparation of essence so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a stem cell exosome, wherein the stem cell exosome is a skin stem cell exosome, and a preparation method of the stem cell exosome comprises the following steps:
s1: taking human skin stem cells for later use;
s2: mixing the skin stem cells with the electrotransfection working solution to obtain a mixed solution, and mixing the mixed solution with CTF1 plasmid to obtain a pre-transfection product;
s3: carrying out electric shock on the pre-transfection object to obtain skin stem cells transfected with CTF1 genes;
s4: conventionally culturing the skin stem cells transfected with the CTF1 gene, and collecting skin stem cell exosomes;
s5: and centrifuging to obtain the finished product of the skin stem cell exosome.
As a further scheme of the invention: the skin stem cells are added to the essence as a component.
As a still further scheme of the invention: essence containing stem cell exosomes comprises the following components: xylitol-based glucoside, dehydrated xylitol, sodium hyaluronate, stem cell exosomes, purslane extract, ceramide 3 and beta-glucose, wherein the components in parts by weight are as follows: 10-15 parts of xylitol-based glucoside, 20-30 parts of dehydrated xylitol, 30-50 parts of xylitol, 5-10 parts of sodium hyaluronate, 10-15 parts of dry cell exosome, 5-10 parts of purslane extract, 35-10 parts of ceramide and 5-10 parts of beta-glucose.
As a still further scheme of the invention: essence containing stem cell exosomes comprises the following components: xylitol-based glucoside, dehydrated xylitol, sodium hyaluronate, stem cell exosomes, purslane extract, ceramide 3 and beta-glucose, wherein the components in parts by weight are as follows: 11-14 parts of xylitol-based glucoside, 22-28 parts of dehydrated xylitol, 32-48 parts of xylitol, 6-9 parts of sodium hyaluronate, 11-14 parts of dry cell exosome, 6-9 parts of purslane extract, 36-9 parts of ceramide and 6-9 parts of beta-glucose.
As a still further scheme of the invention: essence containing stem cell exosomes comprises the following components: xylitol-based glucoside, dehydrated xylitol, sodium hyaluronate, stem cell exosomes, purslane extract, ceramide 3 and beta-glucose, wherein the components in parts by weight are as follows: 12 parts of xylitol-based glucoside, 15 parts of dehydrated xylitol, 40 parts of xylitol, 7 parts of sodium hyaluronate, 12 parts of dry cell exosomes, 7 parts of purslane extract, 37 parts of ceramide and 7 parts of beta-glucose.
As a still further scheme of the invention: the preparation method of the dehydrated xylitol comprises the following steps: preparing mixed acid by using sulfuric acid and p-toluic acid, putting the xylitol and the mixed acid into a reaction vat, and carrying out dehydration reaction for 30min at the temperature of 200-.
As a still further scheme of the invention: the preparation method of the essence containing the stem cell exosomes comprises the following steps:
s1: preparing a stem cell exosome;
s2: preparation of dehydrated xylitol
S3: taking the raw materials in parts by weight, wherein the raw materials comprise: 12 parts of xylitol-based glucoside, 15 parts of dehydrated xylitol, 40 parts of xylitol, 7 parts of sodium hyaluronate, 12 parts of dry cell exosomes, 7 parts of purslane extract, 37 parts of ceramide and 7 parts of beta-glucose;
s4: heating xylitol-based glucoside, dehydrated xylitol, xylitol and sodium hyaluronate to 70-75 ℃, and dissolving until the xylitol-based glucoside, dehydrated xylitol, xylitol and sodium hyaluronate are transparent to obtain a first mixed solution;
s5: cooling the first mixed solution, adding the stem cell exosomes, and uniformly stirring and mixing to obtain a second mixed solution;
s6: standing the second mixed solution for 30min, adding herba Portulacae extract, ceramide and beta-glucose, stirring, mixing, and freeze drying to obtain product with water content less than 3%.
Compared with the prior art, the invention has the beneficial effects that:
this stem cell exosome is with skin stem cell exosome for in the essence, improvement that can be very big, the essence is to the lock water ability of skin, improves the fine and smooth degree of skin, has added modified xylitol moreover, further increases the effect, it is used for preparing the essence to have solved skin stem cell exosome, the maintenance of improvement skin moisture that can be very big, the fine and smooth degree of improvement skin, but present essence does not use stem cell exosome, can't satisfy the problem of the demand of people to the lock water ability of skin.
Detailed Description
The technical solution of the present invention will be described in further detail with reference to specific embodiments.
Example 1
A preparation method of essence containing stem cell exosomes comprises the following steps:
s1: preparing a stem cell exosome: taking human skin stem cells for later use; mixing the skin stem cells with the electrotransfection working solution to obtain a mixed solution, and mixing the mixed solution with CTF1 plasmid to obtain a pre-transfection product; carrying out electric shock on the pre-transfection object to obtain skin stem cells transfected with CTF1 genes; conventionally culturing the skin stem cells transfected with the CTF1 gene, and collecting skin stem cell exosomes; centrifuging to obtain finished skin stem cell exosomes;
s2: preparing dehydrated xylitol: preparing mixed acid by using sulfuric acid and p-toluic acid, putting the xylitol and the mixed acid into a reaction vat, and carrying out dehydration reaction for 30min at the temperature of 200-;
s3: taking the raw materials in parts by weight, wherein the raw materials comprise: 12 parts of xylitol-based glucoside, 15 parts of dehydrated xylitol, 40 parts of xylitol, 7 parts of sodium hyaluronate, 12 parts of dry cell exosomes, 7 parts of purslane extract, 37 parts of ceramide and 7 parts of beta-glucose;
s4: heating xylitol-based glucoside, dehydrated xylitol, xylitol and sodium hyaluronate to 70-75 ℃, and dissolving until the xylitol-based glucoside, dehydrated xylitol, xylitol and sodium hyaluronate are transparent to obtain a first mixed solution;
s5: cooling the first mixed solution, adding the stem cell exosomes, and uniformly stirring and mixing to obtain a second mixed solution;
s6: standing the second mixed solution for 30min, adding herba Portulacae extract, ceramide and beta-glucose, stirring, mixing, and freeze drying to obtain product with water content less than 3%.
Example 2
A preparation method of essence containing stem cell exosomes comprises the following steps:
s1: preparing a stem cell exosome: taking human skin stem cells for later use; mixing the skin stem cells with the electrotransfection working solution to obtain a mixed solution, and mixing the mixed solution with CTF1 plasmid to obtain a pre-transfection product; carrying out electric shock on the pre-transfection object to obtain skin stem cells transfected with CTF1 genes; conventionally culturing the skin stem cells transfected with the CTF1 gene, and collecting skin stem cell exosomes; centrifuging to obtain finished skin stem cell exosomes;
s2: preparing dehydrated xylitol: preparing mixed acid by using sulfuric acid and p-toluic acid, putting the xylitol and the mixed acid into a reaction vat, and carrying out dehydration reaction for 30min at the temperature of 200-;
s3: taking the raw materials in parts by weight, wherein the raw materials comprise: 10 parts of xylitol-based glucoside, 20 parts of dehydrated xylitol, 30 parts of xylitol, 5 parts of sodium hyaluronate, 10 parts of dry cell exosomes, 5 parts of purslane extract, 35 parts of ceramide and 5 parts of beta-glucose;
s4: heating xylitol-based glucoside, dehydrated xylitol, xylitol and sodium hyaluronate to 70-75 ℃, and dissolving until the xylitol-based glucoside, dehydrated xylitol, xylitol and sodium hyaluronate are transparent to obtain a first mixed solution;
s5: cooling the first mixed solution, adding the stem cell exosomes, and uniformly stirring and mixing to obtain a second mixed solution;
s6: standing the second mixed solution for 30min, adding herba Portulacae extract, ceramide and beta-glucose, stirring, mixing, and freeze drying to obtain product with water content less than 3%.
Example 3
A preparation method of essence containing stem cell exosomes comprises the following steps:
s1: preparing a stem cell exosome: taking human skin stem cells for later use; mixing the skin stem cells with the electrotransfection working solution to obtain a mixed solution, and mixing the mixed solution with CTF1 plasmid to obtain a pre-transfection product; carrying out electric shock on the pre-transfection object to obtain skin stem cells transfected with CTF1 genes; conventionally culturing the skin stem cells transfected with the CTF1 gene, and collecting skin stem cell exosomes; centrifuging to obtain finished skin stem cell exosomes;
s2: preparing dehydrated xylitol: preparing mixed acid by using sulfuric acid and p-toluic acid, putting the xylitol and the mixed acid into a reaction vat, and carrying out dehydration reaction for 30min at the temperature of 200-;
s3: taking the raw materials in parts by weight, wherein the raw materials comprise: 15 parts of xylitol-based glucoside, 30 parts of dehydrated xylitol, 50 parts of xylitol, 10 parts of sodium hyaluronate, 15 parts of dry cell exosomes, 10 parts of purslane extract, 310 parts of ceramide and 10 parts of beta-glucose;
s4: heating xylitol-based glucoside, dehydrated xylitol, xylitol and sodium hyaluronate to 70-75 ℃, and dissolving until the xylitol-based glucoside, dehydrated xylitol, xylitol and sodium hyaluronate are transparent to obtain a first mixed solution;
s5: cooling the first mixed solution, adding the stem cell exosomes, and uniformly stirring and mixing to obtain a second mixed solution;
s6: standing the second mixed solution for 30min, adding herba Portulacae extract, ceramide and beta-glucose, stirring, mixing, and freeze drying to obtain product with water content less than 3%.
Example 4
A preparation method of essence containing stem cell exosomes comprises the following steps:
s1: preparing a stem cell exosome: taking human skin stem cells for later use; mixing the skin stem cells with the electrotransfection working solution to obtain a mixed solution, and mixing the mixed solution with CTF1 plasmid to obtain a pre-transfection product; carrying out electric shock on the pre-transfection object to obtain skin stem cells transfected with CTF1 genes; conventionally culturing the skin stem cells transfected with the CTF1 gene, and collecting skin stem cell exosomes; centrifuging to obtain finished skin stem cell exosomes;
s2: preparing dehydrated xylitol: preparing mixed acid by using sulfuric acid and p-toluic acid, putting the xylitol and the mixed acid into a reaction vat, and carrying out dehydration reaction for 30min at the temperature of 200-;
s3: taking the raw materials in parts by weight, wherein the raw materials comprise: 11 parts of xylitol-based glucoside, 22 parts of dehydrated xylitol, 32 parts of xylitol, 6 parts of sodium hyaluronate, 11 parts of dry cell exosomes, 6 parts of purslane extract, 36 parts of ceramide and 6 parts of beta-glucose;
s4: heating xylitol-based glucoside, dehydrated xylitol, xylitol and sodium hyaluronate to 70-75 ℃, and dissolving until the xylitol-based glucoside, dehydrated xylitol, xylitol and sodium hyaluronate are transparent to obtain a first mixed solution;
s5: cooling the first mixed solution, adding the stem cell exosomes, and uniformly stirring and mixing to obtain a second mixed solution;
s6: standing the second mixed solution for 30min, adding herba Portulacae extract, ceramide and beta-glucose, stirring, mixing, and freeze drying to obtain product with water content less than 3%.
Example 5
A preparation method of essence containing stem cell exosomes comprises the following steps:
s1: preparing a stem cell exosome: taking human skin stem cells for later use; mixing the skin stem cells with the electrotransfection working solution to obtain a mixed solution, and mixing the mixed solution with CTF1 plasmid to obtain a pre-transfection product; carrying out electric shock on the pre-transfection object to obtain skin stem cells transfected with CTF1 genes; conventionally culturing the skin stem cells transfected with the CTF1 gene, and collecting skin stem cell exosomes; centrifuging to obtain finished skin stem cell exosomes;
s2: preparing dehydrated xylitol: preparing mixed acid by using sulfuric acid and p-toluic acid, putting the xylitol and the mixed acid into a reaction vat, and carrying out dehydration reaction for 30min at the temperature of 200-;
s3: taking the raw materials in parts by weight, wherein the raw materials comprise: 14 parts of xylitol-based glucoside, 28 parts of dehydrated xylitol, 48 parts of xylitol, 9 parts of sodium hyaluronate, 14 parts of dry cell exosomes, 9 parts of purslane extract, 39 parts of ceramide and 9 parts of beta-glucose;
s4: heating xylitol-based glucoside, dehydrated xylitol, xylitol and sodium hyaluronate to 70-75 ℃, and dissolving until the xylitol-based glucoside, dehydrated xylitol, xylitol and sodium hyaluronate are transparent to obtain a first mixed solution;
s5: cooling the first mixed solution, adding the stem cell exosomes, and uniformly stirring and mixing to obtain a second mixed solution;
s6: standing the second mixed solution for 30min, adding herba Portulacae extract, ceramide and beta-glucose, stirring, mixing, and freeze drying to obtain product with water content less than 3%.
While the preferred embodiments of the present invention have been described in detail, the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.
Claims (7)
1. A stem cell exosome, wherein the stem cell exosome is a skin stem cell exosome, and a preparation method of the stem cell exosome comprises the following steps:
s1: taking human skin stem cells for later use;
s2: mixing the skin stem cells with the electrotransfection working solution to obtain a mixed solution, and mixing the mixed solution with CTF1 plasmid to obtain a pre-transfection product;
s3: carrying out electric shock on the pre-transfection object to obtain skin stem cells transfected with CTF1 genes;
s4: conventionally culturing the skin stem cells transfected with the CTF1 gene, and collecting skin stem cell exosomes;
s5: and centrifuging to obtain the finished product of the skin stem cell exosome.
2. Use of the stem cell exosome according to claim 1 for preparing an essence, wherein the skin stem cells are added as a component to the essence.
3. The essence containing stem cell exosomes according to claim 2, comprising the following components: xylitol-based glucoside, dehydrated xylitol, sodium hyaluronate, stem cell exosomes, purslane extract, ceramide 3 and beta-glucose, wherein the components in parts by weight are as follows:
10-15 parts of xylitol-based glucoside, 20-30 parts of dehydrated xylitol, 30-50 parts of xylitol, 5-10 parts of sodium hyaluronate, 10-15 parts of dry cell exosome, 5-10 parts of purslane extract, 35-10 parts of ceramide and 5-10 parts of beta-glucose.
4. The essence containing stem cell exosomes according to claim 3, comprising the following components: xylitol-based glucoside, dehydrated xylitol, sodium hyaluronate, stem cell exosomes, purslane extract, ceramide 3 and beta-glucose, wherein the components in parts by weight are as follows:
11-14 parts of xylitol-based glucoside, 22-28 parts of dehydrated xylitol, 32-48 parts of xylitol, 6-9 parts of sodium hyaluronate, 11-14 parts of dry cell exosome, 6-9 parts of purslane extract, 36-9 parts of ceramide and 6-9 parts of beta-glucose.
5. The essence containing stem cell exosomes according to claim 3, comprising the following components: xylitol-based glucoside, dehydrated xylitol, sodium hyaluronate, stem cell exosomes, purslane extract, ceramide 3 and beta-glucose, wherein the components in parts by weight are as follows:
12 parts of xylitol-based glucoside, 15 parts of dehydrated xylitol, 40 parts of xylitol, 7 parts of sodium hyaluronate, 12 parts of dry cell exosomes, 7 parts of purslane extract, 37 parts of ceramide and 7 parts of beta-glucose.
6. The essence containing stem cell exosomes according to claim 3, wherein the dehydrated xylitol is prepared by the following method: preparing mixed acid by using sulfuric acid and p-toluic acid, putting the xylitol and the mixed acid into a reaction vat, and carrying out dehydration reaction for 30min at the temperature of 200-.
7. A method for preparing essence containing stem cell exosomes according to claim 3, comprising the steps of:
s1: preparing a stem cell exosome;
s2: preparation of dehydrated xylitol
S3: taking the raw materials in parts by weight, wherein the raw materials comprise: 12 parts of xylitol-based glucoside, 15 parts of dehydrated xylitol, 40 parts of xylitol, 7 parts of sodium hyaluronate, 12 parts of dry cell exosomes, 7 parts of purslane extract, 37 parts of ceramide and 7 parts of beta-glucose;
s4: heating xylitol-based glucoside, dehydrated xylitol, xylitol and sodium hyaluronate to 70-75 ℃, and dissolving until the xylitol-based glucoside, dehydrated xylitol, xylitol and sodium hyaluronate are transparent to obtain a first mixed solution;
s5: cooling the first mixed solution, adding the stem cell exosomes, and uniformly stirring and mixing to obtain a second mixed solution;
s6: standing the second mixed solution for 30min, adding herba Portulacae extract, ceramide and beta-glucose, stirring, mixing, and freeze drying to obtain product with water content less than 3%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113304097A (en) * | 2021-05-27 | 2021-08-27 | 上海南滨江细胞生物科技有限公司 | Essence containing stem cell exosomes and preparation method thereof |
| CN113925825A (en) * | 2021-11-18 | 2022-01-14 | 张�杰 | Facial essence and preparation method thereof |
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2021
- 2021-02-01 CN CN202110135477.0A patent/CN112662630A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113304097A (en) * | 2021-05-27 | 2021-08-27 | 上海南滨江细胞生物科技有限公司 | Essence containing stem cell exosomes and preparation method thereof |
| CN113925825A (en) * | 2021-11-18 | 2022-01-14 | 张�杰 | Facial essence and preparation method thereof |
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