CN113303401A - Preparation method of piglet feed additive utilizing whole coix plant - Google Patents
Preparation method of piglet feed additive utilizing whole coix plant Download PDFInfo
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- CN113303401A CN113303401A CN202110653727.XA CN202110653727A CN113303401A CN 113303401 A CN113303401 A CN 113303401A CN 202110653727 A CN202110653727 A CN 202110653727A CN 113303401 A CN113303401 A CN 113303401A
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- 239000003674 animal food additive Substances 0.000 title claims abstract description 39
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- NJKOMDUNNDKEAI-UHFFFAOYSA-N beta-sitosterol Natural products CCC(CCC(C)C1CCC2(C)C3CC=C4CC(O)CCC4C3CCC12C)C(C)C NJKOMDUNNDKEAI-UHFFFAOYSA-N 0.000 description 2
- 230000001914 calming effect Effects 0.000 description 2
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- XBZYWSMVVKYHQN-MYPRUECHSA-N (4as,6as,6br,8ar,9r,10s,12ar,12br,14bs)-10-hydroxy-2,2,6a,6b,9,12a-hexamethyl-9-[(sulfooxy)methyl]-1,2,3,4,4a,5,6,6a,6b,7,8,8a,9,10,11,12,12a,12b,13,14b-icosahydropicene-4a-carboxylic acid Chemical compound C1C[C@H](O)[C@@](C)(COS(O)(=O)=O)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CCC(C)(C)C[C@H]5C4=CC[C@@H]3[C@]21C XBZYWSMVVKYHQN-MYPRUECHSA-N 0.000 description 1
- DPQCZNIGGNJGTD-UHFFFAOYSA-N Chloride-(??)-2-Methylpentanoic acid Natural products CCCCCCC=CCCCCCCCCCC(=O)OC(C)C(C)OC(=O)CCCCCCCC=CCCCCCC DPQCZNIGGNJGTD-UHFFFAOYSA-N 0.000 description 1
- DPQCZNIGGNJGTD-MVCBGFDASA-N Coixenolide Chemical compound CCCCCC\C=C\CCCCCCCCCC(=O)O[C@@H](C)[C@@H](C)OC(=O)CCCCCCC\C=C/CCCCCC DPQCZNIGGNJGTD-MVCBGFDASA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
Abstract
The invention discloses a preparation method of a piglet feed additive utilizing whole coix plants, which comprises the steps of cleaning fresh coix roots and coix stems and leaves, cutting the fresh coix roots and coix stems and leaves, retting the cut coix stems and leaves for 2 to 3 weeks, inoculating a strain of crinkled cap mushrooms to perform fungus fermentation culture for 3 to 4 weeks, soaking and freezing the strain and the puffed coix seeds together, performing reflux extraction on the strain and the puffed coix seeds by using 60 to 90 weight percent of ethanol solution and drinking water in sequence, combining extracting solutions, and recovering residues; concentrating the extractive solution, spray drying to obtain dry powder, inoculating the residue to strain of Pleurotus rugulosus, performing fungal fermentation culture for 3-4 weeks, oven drying, and pulverizing; and finally extracting, concentrating, spray drying and processing to form dry powder, uniformly mixing the dry powder with the residue powder obtained by fungus fermentation culture and pulverization, and packaging. The preparation process is scientific and reasonable, the preparation process has strong operability, and the prepared additive has good palatability, can effectively improve the digestion and absorption capacity of piglets, reduce the feed-meat ratio, enhance the immunity and the anti-stress capacity, and promote the growth of the piglets.
Description
Technical Field
The invention belongs to the technical field of piglet feed, and particularly relates to a preparation method of a piglet feed additive utilizing complete coix seeds.
Background
The current chemical drugs and antibiotics are widely and at high dose used in veterinary clinics, and have serious negative effects on animal health, human health and environment while promoting piglet growth and improving breeding benefits, the application of the chemical drugs and antibiotics in livestock husbandry is completely forbidden in European Union and America at present, and the feed additives of the chemical drugs and the antibiotics are completely forbidden in China from 2021, so that the research and development of the pollution-free, residue-free and multifunctional feed additives capable of effectively replacing the chemical drugs becomes a key technical problem which is urgently needed to be solved by the livestock industry. The Chinese herbal medicine including the coix seeds is a natural green plant, has two attributes of nutrition and medicine, has been applied to the raising of piglets, has the functions of promoting the growth and development of animals, improving the utilization rate of feed and the like, and is one of the main technical means of 'feed resistance prohibition and feed resistance reduction' at present.
The coix seed is a traditional Chinese medicinal material and coarse cereals which are used as both medicine and food in China, the coix seed is commonly called coix seed, is rich in nutrition, is rich in nutrient components such as starch, protein, fat, amino acid, vitamin and the like, simultaneously contains active components such as coix seed ester, polysaccharide, beta-sitosterol, triterpenoid and the like, and has the effects of promoting diuresis and excreting dampness, strengthening spleen and stopping diarrhea, promoting immunity and the like. The coix seed plants are tall and big, the coix seed grains (also called coix seeds, containing coix seeds, coix shells and coix seed coats) only account for about 20 percent of the field yield of the coix seeds, and the coix seed plants also contain the agricultural byproducts such as coix roots, coix stems, coix leaves and the like with the yield about 4 times, the agricultural byproducts are usually discarded as wastes in the planting production of the coix seeds (coix seeds), the coix byproducts keep all or partial nutrition and active ingredients of the coix seeds, particularly contain the active ingredient coixol which is not contained or contains a small amount of the coix seeds (coix seeds), and the coixol has good effects of calming, clearing away heat and toxic materials, resisting bacteria and diminishing inflammation and the like. The coix seed byproducts can be processed into feed, particularly into a piglet feed additive, so that the production problems of weak digestion and absorption, high diarrhea rate, more stress reactions, slow growth and the like of piglets can be well solved, the utilization rate of the feed is improved, the growth and development of the piglets are promoted, the synergism and the sustainable development of the coix seed industry are promoted, and the feed has good economic, social and ecological benefits.
Disclosure of Invention
The invention aims to provide a preparation method of a piglet feed additive by utilizing the whole plant of coix lacryma-jobi, the preparation process is scientific and reasonable, the operability of the preparation process is strong, the palatability of the prepared additive is good, the digestion and absorption capacity of piglets can be effectively improved, the feed conversion ratio is reduced, the immunity and the anti-stress capacity are enhanced, and the growth of the piglets is promoted.
The piglet feed additive for the whole plant utilization of the coix, disclosed by the invention, comprises the following raw material components in parts by weight: 10-30 parts of coix seed, 10-30 parts of coix root and 50-70 parts of coix stem leaf.
The preparation method comprises the steps of cleaning fresh roots and stems and leaves of the coix seeds, cutting the roots and leaves, composting for 2-3 weeks, inoculating a strain of the crinkled stropharia rugoso-annulata to perform fungus fermentation culture for 3-4 weeks, soaking and freezing the strain and the puffed coix seeds, performing reflux extraction on the frozen coix seeds and the puffed coix seeds by using 60-90 wt% of ethanol solution and drinking water in sequence, combining extracting solutions, and recovering residues; concentrating the extractive solution, spray drying to obtain dry powder, inoculating the residue to strain of Pleurotus rugulosus, performing fungal fermentation culture for 3-4 weeks, oven drying, and pulverizing; and finally extracting, concentrating, spray drying and processing to form dry powder, uniformly mixing the dry powder with the residue powder obtained by fungus fermentation culture and pulverization, and packaging. The method comprises the following specific steps:
1, selecting materials: removing rotten and abnormal parts and impurities in the raw materials of the coix seed, the coix root and the coix stem leaf for later use;
2, puffing: puffing Coicis semen with air-flow type popcorn puffing machine under 0.5-1.2Mpa for 5-9 min;
3, fermenting and culturing fungi: cutting fresh roots and stems and leaves of coix lacryma-jobi into small segments of 1-3cm by a weeding machine, controlling the middle temperature of a material pile to be 60-80 ℃, controlling the water content to be 50-70wt%, retting the material pile for 2-3 weeks, spreading and cooling to 40-50 ℃, then inoculating the solid production seeds of the rugose annulate into a sterile plastic bag or a container with a breather valve according to the weight ratio of 100:2-5, uniformly stirring, putting the sterile plastic bag or the container into the sterile plastic bag or the container with the breather valve, culturing at the temperature of 20-30 ℃ and the humidity of 50-70%, fermenting and culturing fungi for 3-4 weeks, and drying the mixture for later use;
4, soaking: mixing the puffed coix seed with the coix seed root, coix seed stem and leaf which are subjected to fermentation culture and sun drying, adding drinking water according to the solid-liquid weight ratio of 1:2-5, soaking for 10-15h, taking out and draining for later use;
5, freezing: freezing Coicis semen, Coicis semen root and Coicis semen stem and leaf in freezer of-5-10 deg.C for 15-24 hr, and naturally thawing at room temperature for use;
6, leaching: freezing coix seed, coix root and coix stem leaf according to the weight-volume ratio of 1: adding 60-90 wt% ethanol solution into 4-8 g/ml, reflux extracting for 0.5-1.5 hr, collecting extractive solution a, and mixing the residue at a weight volume ratio of 1: adding 4-8 g/ml drinking water, reflux extracting at 85-95 deg.C for 2-3 times, each time for 0.5-1.5 hr, collecting extractive solution b, and collecting residue;
7, concentrating: mixing the extractive solutions a and b, and concentrating to relative density of 1.05-1.10 at 40-60 deg.C;
8, spray drying: adding dextrin which accounts for 1-3% of the weight of the concentrated solution into the obtained concentrated solution, uniformly stirring, carrying out spray drying, and collecting dry powder for later use;
9, secondary fungus fermentation culture: transferring the extracted residue to an aseptic inoculation chamber, inoculating the strain of the crinkled stropharia rugoso-annulata to the strain production chamber according to the weight ratio of 100:1-3 when the extracted residue is cooled to 40-50 ℃, uniformly stirring, putting the strain production chamber into an aseptic plastic bag or a container with a breather valve, culturing at the temperature of 20-30 ℃ and the humidity of 50-70%, and fermenting and culturing fungi for 3-4 weeks for later use;
10, drying and crushing: drying and pulverizing the extraction residue after the fungus fermentation culture, and sieving with a sieve of 80-160 meshes for later use;
11, mixing: and uniformly mixing the spray-dried dry powder with the dried and crushed extraction residue powder, and packaging to obtain the feed additive.
Remarking: the production strain is a solid strain which is directly used in the production of edible fungi, and is prepared by inoculating strains into a sterilized culture base material, culturing hyphae, and inoculating the edible fungi when the hyphae fully fill the whole bag of culture medium.
The piglet feed additive disclosed by the invention is applied as follows:
the additive is added into daily feed (powder) of piglets according to the proportion that the weight ratio of the feed amount of the piglets is 1-3% by weight, the feed is carried out after the mixture is evenly stirred, the feed can also be added into basal ration formula of the piglets in the form of premix and granulated into granulated feed for feeding, and the feed additive is added into the piglets from the weaning of the piglets without interruption until the piglets grow to about 40 kg.
The invention has the advantages that:
1. the piglet feed additive prepared by the invention not only contains all nutritional ingredients and nutritional values of the whole coix seed plant, but also has medicinal ingredients and effects of the whole coix seed plant, is rich in active ingredients such as coixenolide, coixol, coix seed polysaccharide, beta-sitosterol, triterpenoids and the like, has the effects of strengthening spleen, relieving diarrhea, calming, diminishing inflammation, resisting bacteria, clearing damp, clearing heat, detoxifying and the like, and has good nutritional health-care effects of promoting digestion and absorption of piglets, resisting stress, preventing and treating diarrhea of piglets and the like.
2. The preparation process of the invention is scientific and reasonable, and the preparation process has strong operability. According to the invention, the raw materials of the coix seed, the coix root, the coix stem leaf and the like are puffed, soaked and frozen, and then an extraction method of extracting alcohol and then extracting water is adopted, so that fat-soluble and water-soluble nutritional and medicinal active ingredients in the whole coix seed plants are extracted to the maximum extent, and the effect of the piglet feed additive utilized by the whole coix seed plants is ensured to the maximum extent.
3. Cutting roots and stems of coix seeds, composting and inoculating a strain of the crinkled cap mushrooms to the roots and stems of the coix seeds for fermentation culture, converting most of crude fibers in the roots, the stems and the stems into hyphal proteins through microorganisms, remarkably improving the contents of proteins and amino acids which are respectively 136.36% and 154.80%, remarkably reducing the content of the crude fibers in the roots, the stems and the stems, and reducing the content of the crude fibers by 48.22%; after extraction, the extracted residue is inoculated with the strain of the stropharia rugoso-annulata again for fungus fermentation culture, crude fibers in roots, stems and leaves of the coix lacryma-jobi and the coix lacryma-jobi grains can be further converted into hypha proteins through microorganisms, the contents of the proteins and amino acids are obviously improved by 64.82 percent and 59.09 percent respectively, the content of the crude fibers in the whole coix lacryma-jobi is obviously reduced by 42.37 percent, the nutritional quality of the feed additive is improved, meanwhile, the content of polysaccharides, particularly bacterial polysaccharides, can be obviously improved, the content of the polysaccharides is improved by 37.74 percent, and the immune efficacy of the piglet feed additive utilized by the whole coix lacryma-jobi is enhanced.
TABLE 1 comparison of the content of nutrients and active ingredients before and after fermentation of root, stem and leaf of Coix lacryma-jobi
Note: the different English letters represent that the comparison has significant difference (P < 0.05)
TABLE 2 comparison of nutrients and active ingredient contents before and after fermentation of extraction residue
Note: the different English letters represent that the comparison has significant difference (P < 0.05)
4. The prepared feed additive has good palatability, can effectively improve the digestion and absorption capacity and immunity of piglets, and obviously reduces the feed conversion ratio and diarrhea, thereby promoting the growth of the piglets. Under the same feeding condition, compared with a control group (without the conventional feed additive), the Average Daily Gain (ADG) of the piglets added with the feed additive is improved by 0.02-0.05 kg/head, and the feed-meat ratio is reduced by 11.8-18.5%; has obvious prevention effect on diarrhea of piglets, and the incidence rate of diarrhea is reduced by 54.8-83.2%; meanwhile, the invention has obvious effect of improving the immunity of piglets, the content of immunoglobulin IgA in the serum of the piglets is improved by 15.7-28.5%, the content of immunoglobulin IgM is improved by 13.5-24.2%, and the content of immunoglobulin IgG is improved by 9.8-14.6%. (see in particular the results of the additive feeding experiments hereinafter).
Detailed Description
In order to make the aforementioned and other features and advantages of the invention more comprehensible, embodiments accompanied with figures are described in detail below. The method of the present invention is a method which is conventional in the art unless otherwise specified.
Example 1
A piglet feed additive utilizing the whole coix plant comprises the following raw material components in parts by weight: 10 parts of coix seed, 10 parts of coix root and 50 parts of coix stem leaf.
The preparation method comprises the following specific steps:
1, selecting materials: removing rotten and abnormal parts and impurities in the raw materials of the coix seed, the coix root and the coix stem leaf for later use;
2, puffing: puffing Coicis semen with air-flow type popcorn puffing machine under 0.5Mpa for 5 min;
3, fermenting and culturing fungi: cutting fresh roots and stems and leaves of the coix lacryma-jobi into small segments of 1cm by a weeding machine, controlling the middle temperature of a material pile to be 80 ℃, controlling the water content to be 50wt%, retting the material pile for 2 weeks, spreading and cooling the material pile to 45 ℃, inoculating a strain solid production seed of the crinkled cap fungus according to the weight ratio of 100:2, uniformly stirring the strain solid production seed, putting the strain solid production seed into a sterile plastic bag or a container with a breather valve, culturing the strain in the sterile plastic bag or the container at the temperature of 20 ℃ and the humidity of 70%, fermenting and culturing the fungus for 4 weeks, and drying the strain for later use;
4, soaking: mixing the puffed coix seed with the coix seed roots, coix seed stems and leaves which are subjected to fermentation culture and sun drying, adding drinking water according to the solid-liquid weight ratio of 1:2, soaking for 10h, taking out and draining for later use;
5, freezing: freezing Coicis semen, Coicis semen root and Coicis semen stem and leaf at-5 deg.C for 15 hr, and naturally thawing at room temperature;
6, leaching: freezing coix seed, coix root and coix stem leaf according to the weight-volume ratio of 1: adding 60wt% ethanol solution into 4g/ml, reflux extracting for 0.5 hr, collecting extractive solution a, and mixing the residue at weight volume ratio of 1: adding 4g/ml drinking water, reflux-extracting at 85 deg.C for 0.5 hr for 2 times, collecting extractive solution b, and collecting residue;
7, concentrating: mixing the extractive solutions a and b, and concentrating to relative density of 1.05 at 40 deg.C;
8, spray drying: adding dextrin accounting for 1% of the weight of the concentrated solution into the obtained concentrated solution, uniformly stirring, performing spray drying, and collecting dry powder for later use;
9, secondary fungus fermentation culture: transferring the extracted residues to an aseptic inoculation chamber, inoculating the strain of the crinkled stropharia rugoso-annulata to the strain production chamber according to the weight ratio of 100:1 when the extracted residues are cooled to 40 ℃, uniformly stirring, putting the strain production chamber into an aseptic plastic bag or a container with a breather valve, culturing at the temperature of 20 ℃ and the humidity of 70%, and performing fermentation culture on fungi for 3 weeks for later use;
10, drying and crushing: drying and crushing the extraction residue after the fungus fermentation culture, and sieving the extraction residue with a 80-mesh sieve for later use;
11, mixing: and uniformly mixing the spray-dried dry powder with the dried and crushed extraction residue powder, and packaging to obtain the feed additive.
Example 2
A piglet feed additive utilizing the whole coix plant comprises the following raw material components in parts by weight: 20 parts of coix seed, 20 parts of coix root and 60 parts of coix stem leaf.
The preparation method comprises the following specific steps:
1, selecting materials: removing rotten and abnormal parts and impurities in the raw materials of the coix seed, the coix root and the coix stem leaf for later use;
2, puffing: puffing Coicis semen with airflow type popcorn puffing machine under 1.0Mpa for 7 min;
3, fermenting and culturing fungi: cutting fresh roots and stems and leaves of the coix lacryma-jobi into small 2cm segments by a weeding machine, controlling the middle temperature of a material pile to be 70 ℃, controlling the moisture content to be 60wt%, retting the material pile for 2 weeks, spreading and cooling the material pile to 40 ℃, then inoculating the solid production seeds of the rugose annulate into the material pile according to the weight ratio of 100:3, uniformly stirring the material pile, putting the material pile into a sterile plastic bag or a container with a breather valve, culturing the material pile at the temperature of 30 ℃, keeping the humidity of 50%, fermenting and culturing fungi for 3 weeks, and then drying the fungi for later use;
4, soaking: mixing the puffed coix seed with the coix seed roots, coix seed stems and leaves which are subjected to fermentation culture and sun drying, adding drinking water according to the solid-liquid weight ratio of 1:3, soaking for 12h, taking out and draining for later use;
5, freezing: freezing Coicis semen, Coicis semen root and Coicis semen stem and leaf at-8 deg.C for 20 hr, and naturally thawing at room temperature;
6, leaching: freezing coix seed, coix root and coix stem leaf according to the weight-volume ratio of 1: adding 75wt% ethanol solution into 6g/ml, refluxing and extracting for 1 hour, collecting extract a, and then mixing the residues according to the weight volume ratio of 1: adding drinking water 6g/ml, reflux-extracting at 90 deg.C for 2 times, each for 1 hr, collecting extractive solution b, and collecting residue;
7, concentrating: mixing the extractive solutions a and b, and concentrating to relative density of 1.08 at 50 deg.C;
8, spray drying: adding dextrin accounting for 2% of the weight of the concentrated solution into the obtained concentrated solution, uniformly stirring, performing spray drying, and collecting dry powder for later use;
9, secondary fungus fermentation culture: transferring the extracted residues to an aseptic inoculation chamber, inoculating the strain of the crinkled cap mushrooms according to the weight ratio of 100:2 when the residues are cooled to 45 ℃, uniformly stirring, putting the strain into an aseptic plastic bag or a container with a breather valve, culturing at the temperature of 30 ℃ and the humidity of 50%, and fermenting and culturing fungi for 3 weeks for later use;
10, drying and crushing: drying and crushing the extraction residue after the fungus fermentation culture, and sieving the extraction residue with a 120-mesh sieve for later use;
11, mixing: and uniformly mixing the spray-dried dry powder with the dried and crushed extraction residue powder, and packaging to obtain the feed additive.
Example 3
A piglet feed additive utilizing the whole coix plant comprises the following raw material components in parts by weight: 30 parts of coix seed, 30 parts of coix root and 70 parts of coix stem leaf.
The preparation method comprises the following specific steps:
1, selecting materials: removing rotten and abnormal parts and impurities in the raw materials of the coix seed, the coix root and the coix stem leaf for later use;
2, puffing: puffing Coicis semen with airflow type popcorn puffing machine under 1.2Mpa for 9 min;
3, fermenting and culturing fungi: cutting fresh roots and stems and leaves of the coix lacryma-jobi into small segments of 3cm by a weeding machine, controlling the middle temperature of a material pile to be 60 ℃, controlling the moisture content to be 70wt%, retting the material pile for 3 weeks, spreading and cooling the material pile to 50 ℃, then inoculating a crinkled stropharia rugoso-annulata solid production seed according to the weight ratio of 100:5, uniformly stirring the seed, putting the seed into a sterile plastic bag or a container with a breather valve, culturing the seed at the temperature of 25 ℃, the humidity of 60%, fermenting and culturing fungi for 4 weeks, and then drying the seeds for later use;
the water content is 50-70wt%, the compost is retted for 2-3 weeks, and then the crinkled cap mushrooms are inoculated according to the weight ratio of 100: 2-5;
4, soaking: mixing the puffed coix seed with the coix seed roots, coix seed stems and leaves which are subjected to fermentation culture and sun drying, adding drinking water according to the solid-liquid weight ratio of 1:5, soaking for 15h, taking out and draining for later use;
5, freezing: freezing Coicis semen, Coicis semen root and Coicis semen stem and leaf at-10 deg.C for 24 hr, and naturally thawing at room temperature;
6, leaching: freezing coix seed, coix root and coix stem leaf according to the weight-volume ratio of 1: adding 90wt% ethanol solution into 8 g/ml, reflux extracting for 1.5 hours, collecting extract a, and mixing the residues according to the weight volume ratio of 1: adding 8 g/ml drinking water, reflux-extracting at 95 deg.C for 3 times, each for 1.5 hr, collecting extractive solution b, and collecting residue;
7, concentrating: mixing the extractive solutions a and b, and concentrating to relative density of 1.10 at 60 deg.C;
8, spray drying: adding dextrin which accounts for 3 percent of the weight of the concentrated solution into the obtained concentrated solution, uniformly stirring, carrying out spray drying, and collecting dry powder for later use;
9, secondary fungus fermentation culture: transferring the extracted residues to an aseptic inoculation chamber, inoculating the strain of the crinkled cap mushrooms according to the weight ratio of 100:3 when the residues are cooled to 50 ℃, uniformly stirring, putting the strain into an aseptic plastic bag or a container with a breather valve, culturing at the temperature of 25 ℃ and the humidity of 60%, and fermenting and culturing fungi for 4 weeks for later use;
10, drying and crushing: drying and crushing the extraction residue after the fungus fermentation culture, and sieving the extraction residue with a 160-mesh sieve for later use;
11, mixing: and uniformly mixing the spray-dried dry powder with the dried and crushed extraction residue powder, and packaging to obtain the feed additive.
Feed additive feeding test with different dosages
The feed additive prepared by the method is added and fed according to different dosages, and is compared with the conventional feeding (without the feed additive).
1. Design of experiments
The test was divided into 3 feed additive test groups (1%, 2% and 3% feed additive added respectively) and a control group (no feed additive added) for 4 treatments, each treatment was set to 3 replicates, each replicate for 10.
The trial was scheduled for feeding 28d (4 weeks) with 5 days of pre-trial. The same batch of 120 healthy piglets 3-5d after weaning are selected for the test, the average weight is 10.12 +/-0.30 kg, the piglets are marked with the ear numbers (or other marks) one by one, and the piglets are selected and classified according to the following principle: (1) sex: the male pigs need to be castrated in each half of the male and female pigs; (2) variety: an outer ternary element; (3) the weights are consistent as much as possible, and according to the weight grades, the grade distribution of the piglets of each test group is consistent with the number of the piglets.
The basic ration is prepared according to the recommended nutritional requirement of NRC (1998) pork pigs, the composition and nutritional level of the basic ration in a specific test pig farm are shown in Table 2, and the feed additive is directly added into the basic ration according to the proportion of 1%, 2% and 3% and is mixed for feeding. During the test period, the feeding is divided into 2 times according to the proportion every day, the amount is adjusted every day, and less feeding are achieved as much as possible. The piglets are fed and drunk freely, and other feeding management and immunization programs are consistent with those of a pig farm.
TABLE 3 basic diet and nutritional ingredients
The test pigs were weighed on an empty stomach and bled on a 08: 00 day by day basis on test 1, 15 and 29 morning, respectively. The feeding and diarrhea episodes were recorded throughout (daily) the test pigs.
Average Daily Gain (ADG) (final-initial group gross)/(days of experiment × group number);
feed-to-meat ratio (F/G) ═ Average Daily Feed Intake (ADFI)/Average Daily Gain (ADG);
diarrhea incidence (%) × (number of heads diarrhea per group × number of days diarrhea)/(number of heads per group × number of days of the trial) × 100;
2. results and analysis
TABLE 4 results of the effect of feed additives on growth performance, diarrhea and immune index of weaned piglets
Note: the different English letters represent that the comparison has significant difference (P < 0.05)
From table 4, it can be derived: compared with a control group, under the same feeding condition, compared with the control group (without the conventional feed additive), the average daily gain of the treatment groups with different dosages of the feed additive is improved to different degrees, and the Average Daily Gain (ADG) is improved by 0.02-0.05 kg/day; the feed conversion ratio is obviously reduced by 11.8 to 18.5 percent; has obvious prevention effect on diarrhea of piglets, and the incidence rate of diarrhea is reduced by 54.8-83.2%; meanwhile, the invention has obvious effect of improving the immunity of piglets, the content of immunoglobulin IgA in the serum of the piglets is improved by 15.7-28.5%, the content of immunoglobulin IgM is improved by 13.5-24.2%, and the content of immunoglobulin IgG is improved by 9.8-14.6%. The result shows that the feed additive can effectively improve the digestion and absorption capacity and immunity of piglets, remarkably reduce the feed-meat ratio and reduce the generation of diarrhea, thereby promoting the growth of the piglets.
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
Claims (2)
1. A piglet feed additive which is utilized by the whole plant of the coix lacryma-jobi is characterized in that: the additive comprises the following raw material components in parts by weight: 10-30 parts of coix seed, 10-30 parts of coix root and 50-70 parts of coix stem leaf.
2. The method for preparing a piglet feed additive by using the whole coix plant according to claim 1, which comprises the following steps: the method comprises the following specific steps:
1, selecting materials: removing rotten and abnormal parts and impurities in the raw materials of the coix seed, the coix root and the coix stem leaf for later use;
2, puffing: puffing Coicis semen with air-flow type popcorn puffing machine under 0.5-1.2Mpa for 5-9 min;
3, fermenting and culturing fungi: cutting fresh roots and stems and leaves of the coix lacryma-jobi into small segments of 1-3cm by a weeding machine, retting the segments for 2-3 weeks, controlling the middle temperature of a stock pile to be 60-80 ℃ and the water content to be 50-70wt%, spreading and cooling the segments to 40-50 ℃, then inoculating the solid production seeds of the stropharia rugoso-annulata according to the weight ratio of 100:2-5, uniformly stirring the seeds, putting the seeds into an aseptic plastic bag or a container with a breather valve, culturing the seeds at the temperature of 20-30 ℃ and the humidity of 50-70%, fermenting and culturing fungi for 3-4 weeks, and drying the seeds for later use;
4, soaking: mixing the puffed coix seed with the coix seed root, coix seed stem and leaf which are subjected to fermentation culture and sun drying, adding drinking water according to the solid-liquid weight ratio of 1:2-5, soaking for 10-15h, taking out and draining for later use;
5, freezing: freezing Coicis semen, Coicis semen root and Coicis semen stem and leaf in freezer of-5-10 deg.C for 15-24 hr, and naturally thawing at room temperature for use;
6, leaching: freezing coix seed, coix root and coix stem leaf according to the weight-volume ratio of 1: adding 60-90 wt% ethanol solution into 4-8 g/ml, reflux extracting for 0.5-1.5 hr, collecting extractive solution a, and mixing the residue at a weight volume ratio of 1: adding 4-8 g/ml drinking water, reflux extracting at 85-95 deg.C for 2-3 times, each time for 0.5-1.5 hr, collecting extractive solution b, and collecting residue;
7, concentrating: mixing the extractive solutions a and b, and concentrating to relative density of 1.05-1.10 at 40-60 deg.C;
8, spray drying: adding dextrin which accounts for 1-3% of the weight of the concentrated solution into the obtained concentrated solution, uniformly stirring, carrying out spray drying, and collecting dry powder for later use;
9, secondary fungus fermentation culture: transferring the extracted residue to an aseptic inoculation chamber, inoculating the strain of the crinkled stropharia rugoso-annulata to the strain production chamber according to the weight ratio of 100:1-3 when the extracted residue is cooled to 40-50 ℃, uniformly stirring, putting the strain production chamber into an aseptic plastic bag or a container with a breather valve, culturing at the temperature of 20-30 ℃ and the humidity of 50-70%, and fermenting and culturing fungi for 3-4 weeks for later use;
10, drying and crushing: drying and pulverizing the extraction residue after the fungus fermentation culture, and sieving with a sieve of 80-160 meshes for later use;
11, mixing: and uniformly mixing the spray-dried dry powder with the dried and crushed extraction residue powder, and packaging to obtain the feed additive.
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CN101023775A (en) * | 2006-02-20 | 2007-08-29 | 郑爱国 | Microbe feed additive |
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CN109601716A (en) * | 2018-12-24 | 2019-04-12 | 福建省农业科学院农业生态研究所 | A kind of feed addictive and preparation method thereof using coix lacryma-jobi processing waste as raw material |
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CN101023775A (en) * | 2006-02-20 | 2007-08-29 | 郑爱国 | Microbe feed additive |
CN106071255A (en) * | 2016-08-17 | 2016-11-09 | 马鞍山市五谷禽业专业合作社 | A kind of minimizing diarrhea rate Poria fermented pig feed additive |
CN109497286A (en) * | 2018-12-24 | 2019-03-22 | 福建省农业科学院农业生态研究所 | A kind of somatotrophic Chinese herbal feed additive of piglet and preparation method thereof |
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