CN113273576A - Platycodon grandiflorum seed coating agent and coating method and application thereof - Google Patents

Platycodon grandiflorum seed coating agent and coating method and application thereof Download PDF

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Publication number
CN113273576A
CN113273576A CN202110492046.XA CN202110492046A CN113273576A CN 113273576 A CN113273576 A CN 113273576A CN 202110492046 A CN202110492046 A CN 202110492046A CN 113273576 A CN113273576 A CN 113273576A
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platycodon grandiflorum
seeds
coating agent
seed
seed coating
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Inventor
王继永
尚兴朴
包芳
邓庭伟
樊良帅
曾燕
张峰
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China Traditional Chinese Medicine Seed & Seedling Co ltd
China National Traditional Chinese Medicine Co ltd
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China Traditional Chinese Medicine Seed & Seedling Co ltd
China National Traditional Chinese Medicine Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/74Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
    • A01N43/781,3-Thiazoles; Hydrogenated 1,3-thiazoles
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/06Coating or dressing seed
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46N-acyl derivatives
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N51/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Soil Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention provides a balloonflower seed coating agent and a coating method and application thereof, the balloonflower seed coating agent consists of 2.78-4.63% of thiabendazole, 0.36-0.60% of metalaxyl-M, 0.46-0.77% of fludioxonil, 1.5% of thiamethoxam, 2.0% of agricultural emulsion 700, 0.2% of xanthan gum, 0.2% of sodium carboxymethyl cellulose, 4% of glycol and purified water, the composition is simple, safe and low-toxic, the production process is simple, the cost is lower, and the balloonflower seed coating agent is suitable for large-scale industrial production; the seed coating agent is added with the antifreezing agent, so that the coated platycodon grandiflorum seeds have certain low temperature resistance, and the germination rate of the platycodon grandiflorum seeds in adverse circumstances can be improved; the coating method is that the balloonflower seed coating agent and the balloonflower seeds are directly mixed and stirred evenly according to a certain medicine seed ratio and then dried, and the coating method is simple; the invention optimizes and verifies the formula of the seed coating agent of the platycodon grandiflorum and the coating method thereof, can effectively solve the problems of pathogenic bacteria carried by the platycodon grandiflorum seeds, seedling diseases and insect pests and the like when used for seedling raising of the platycodon grandiflorum seeds, obviously increases the emergence rate and root length of the platycodon grandiflorum seeds, and improves the germination rate of the seeds and the quality of medicinal materials thereof.

Description

Platycodon grandiflorum seed coating agent and coating method and application thereof
Technical Field
The invention belongs to the technical field of traditional Chinese medicine seed treatment agents, and particularly relates to a platycodon grandiflorum seed coating agent and a coating method and application thereof.
Background
Platycodon grandiflorum is a perennial herb of Campanulaceae, is an economic plant with medicinal, edible and ornamental properties, has the effects of opening and ventilating lung qi, eliminating phlegm and expelling pus, and is mainly distributed in Chifeng of inner Mongolia, Zibo in Shandong, Anhui Bozhou and the like. The platycodon grandiflorum is mainly derived from cultivated medicinal materials and is mainly propagated by seeds, the quality of the seeds is a key factor for determining the high and low yield of the medicinal materials and is also a main condition for determining the quality and quality of the medicinal materials, but the platycodon grandiflorum seeds are easily damaged by the seeds or soil pathogenic bacteria in the germination period and the seedling period, so that the emergence rate and the quality of the medicinal materials are influenced. The seed coating technology can prevent and control plant diseases and insect pests in seedling stage and promote the growth of seedlings, and has obvious economic effect, but the existing seed coating agent is mainly applied to crop seeds, and the research on the seed coating agent of the traditional Chinese medicinal material seeds is less. The seed coating agent for the common crop seeds can not promote the germination of the platycodon grandiflorum seeds, so that the seed coating agent capable of improving the germination rate of the platycodon grandiflorum seeds is needed.
Disclosure of Invention
The invention aims to provide a platycodon grandiflorum seed coating agent, a coating method and application thereof, which are used for solving pathogenic bacteria, seedling diseases and insect pests and the like carried by platycodon grandiflorum seeds and increasing root biomass of platycodon grandiflorum so as to improve the germination rate of the seeds and the quality of medicinal materials.
In order to achieve the technical purpose, the technical scheme adopted by the invention is as follows: a platycodon grandiflorum seed coating agent comprises the following raw materials in percentage by mass: 2.78-4.63% of thiabendazole, 0.36-0.60% of metalaxyl-M, 0.46-0.77% of fludioxonil, 1.5% of thiamethoxam, 2.0% of agricultural emulsion 700, 0.2% of xanthan gum, 0.2% of sodium carboxymethylcellulose, 4% of ethylene glycol and the balance of purified water.
The invention also provides a coating method of the platycodon grandiflorum seed coating agent, which comprises the following steps:
preparing a platycodon root seed coating agent: the material is prepared by mixing the following raw materials in percentage by mass: 2.78-4.63% of thiabendazole, 0.36-0.60% of metalaxyl-M, 0.46-0.77% of fludioxonil, 1.5% of thiamethoxam, 2.0% of agricultural emulsion 700, 0.2% of xanthan gum, 0.2% of sodium carboxymethylcellulose, 4% of ethylene glycol and the balance of purified water;
and (3) uniformly stirring the platycodon root seed coating agent and the platycodon root seeds, and airing to finish the coating of the platycodon root seeds.
Further, the drug seed ratio of the platycodon grandiflorum seed coating agent to the platycodon grandiflorum seeds is 1 mL: 50-90 g.
Further, the platycodon grandiflorum seed coating agent comprises the following raw materials in percentage by mass: 3.71% thiabendazole, 0.48% metalaxyl-M, 0.61% fludioxonil, 1.5% thiamethoxam, 2.0% agricultural milk 700, 0.2% xanthan gum, 0.2% sodium carboxymethylcellulose, 4% ethylene glycol, and the balance of purified water.
Further, the drug seed ratio of the platycodon grandiflorum seed coating agent to the platycodon grandiflorum seeds is 1 mL: 85 g.
The invention also provides application of the platycodon grandiflorum seed coating agent in seedling raising of platycodon grandiflorum seeds.
The invention also provides an application of the coating method of the platycodon grandiflorum seed coating agent in seedling raising of platycodon grandiflorum seeds.
Compared with the prior art, the invention has the following beneficial effects: the invention provides a balloonflower seed coating agent and a coating method and application thereof, the balloonflower seed coating agent consists of 2.78-4.63% of thiabendazole, 0.36-0.60% of metalaxyl-M, 0.46-0.77% of fludioxonil, 1.5% of thiamethoxam, 2.0% of agricultural emulsion 700, 0.2% of xanthan gum, 0.2% of sodium carboxymethylcellulose, 4% of glycol and purified water, and the balloonflower seed coating agent has the advantages of simple components, convenient preparation, safety and low toxicity, simple production process, lower cost and suitability for large-scale industrial production; the film-forming agent and the antifreezing agent are added in the formula of the seed coating agent, the coating treatment of the seed coating agent on the platycodon grandiflorum can improve the film-forming property, uniformity and shedding rate of the coating, and the coated platycodon grandiflorum seed has certain low temperature resistance and can improve the germination rate of the platycodon grandiflorum seed in adverse circumstances; the coating method is that the balloonflower seed coating agent and the balloonflower seeds are directly mixed and stirred evenly according to a certain medicine seed ratio and then dried, and the coating method is simple; the formula of the platycodon grandiflorum seed coating agent and the coating method thereof are optimized and verified, and germination box experiments and sand box experiments show that when the platycodon grandiflorum seeds are treated by using the platycodon grandiflorum seed coating agent and the coating method thereof, the germination rate of the coated platycodon grandiflorum seeds is improved by 4-7 percentage points, and the mildew rate is reduced by 10-13 percentage points; the emergence rate is improved by 5-8 percent, the root is increased by 0.2-0.7 cm, and therefore when the platycodon grandiflorum seed coating agent and the coating method thereof are used for seedling raising of platycodon grandiflorum seeds, pathogenic bacteria, seedling diseases and insect pests and the like carried by the platycodon grandiflorum seeds can be effectively solved, the emergence rate and the root length of the platycodon grandiflorum are increased, and the germination rate of the seeds and the quality of medicinal materials of the seeds are improved.
Drawings
FIG. 1 is a front and rear view of a coated Platycodon grandiflorum seed according to example 2 of the present invention, wherein a is an uncoated Platycodon grandiflorum seed and b is a coated Platycodon grandiflorum seed;
FIG. 2 is a diagram of a germination box for platycodon grandiflorum seeds before and after coating in example 2 of the present invention, wherein a is a diagram of an uncoated platycodon grandiflorum seed germination box, and b is a diagram of a coated platycodon grandiflorum seed germination box;
fig. 3 is a sandbox of the platycodon grandiflorum seeds before and after coating according to example 2 of the present invention, wherein a is a sandbox of the uncoated platycodon grandiflorum seeds, and b is a sandbox of the coated platycodon grandiflorum seeds.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples. The following examples are intended to illustrate the invention only and are not intended to limit the scope of the invention. The experimental procedures used in the following examples are conventional procedures unless otherwise specified, and the reagents and procedures used therein are conventional in the art unless otherwise specified.
The raw material components of the platycodon root seed coating agent in the following embodiments are all expressed by mass percent of effective components.
Example 1
A coating method of a platycodon grandiflorum seed coating agent comprises the following steps:
preparing a platycodon root seed coating agent: is prepared by mixing 2.78 percent of thiabendazole, 0.36 percent of metalaxyl-M, 0.46 percent of fludioxonil, 1.5 percent of thiamethoxam, 2.0 percent of agricultural emulsion 700, 0.2 percent of xanthan gum, 0.2 percent of sodium carboxymethylcellulose and 4 percent of glycol with purified water;
wherein, 2.78 percent of thiabendazole, 0.36 percent of metalaxyl-M, 0.46 percent of fludioxonil and 1.5 percent of thiamethoxam can be obtained by mixing corresponding raw material powder or suspension liquid which is commercially available in the market with purified water;
coating: pouring 1mL of the prepared platycodon grandiflorum seed coating agent into a plastic bag filled with 85g of platycodon grandiflorum seeds, fully shaking and mixing uniformly, and airing to finish the coating of the platycodon grandiflorum seeds.
Example 2
A coating method of a platycodon grandiflorum seed coating agent comprises the following steps:
preparing a platycodon root seed coating agent: is prepared by mixing 3.71 percent of thiabendazole, 0.48 percent of metalaxyl-M, 0.61 percent of fludioxonil, 1.5 percent of thiamethoxam, 2.0 percent of agricultural emulsion 700, 0.2 percent of xanthan gum, 0.2 percent of sodium carboxymethylcellulose and 4 percent of glycol with purified water;
coating: pouring 1mL of the prepared platycodon grandiflorum seed coating agent into a plastic bag filled with 85g of platycodon grandiflorum seeds, fully shaking and mixing uniformly, and drying in the air to complete the coating of the platycodon grandiflorum seeds, wherein the front and the back of the coating of the platycodon grandiflorum seeds in the embodiment are shown in figure 1.
Example 3
A coating method of a platycodon grandiflorum seed coating agent comprises the following steps:
preparing a platycodon root seed coating agent: is prepared by mixing 4.63 percent of thiabendazole, 0.60 percent of metalaxyl-M, 0.77 percent of fludioxonil, 1.5 percent of thiamethoxam, 2.0 percent of agricultural emulsion 700, 0.2 percent of xanthan gum, 0.2 percent of sodium carboxymethylcellulose and 4 percent of glycol with purified water;
coating: pouring 1mL of the prepared platycodon grandiflorum seed coating agent into a plastic bag filled with 85g of platycodon grandiflorum seeds, fully shaking and mixing uniformly, and airing to finish the coating of the platycodon grandiflorum seeds.
Example 4
The present embodiment is different from embodiment 2 in that the mixed seed ratio of the platycodon grandiflorum seed coating agent to the platycodon grandiflorum seeds is 1 mL: 50 g.
Example 5
The present embodiment is different from embodiment 2 in that the mixed seed ratio of the platycodon grandiflorum seed coating agent to the platycodon grandiflorum seeds is 1 mL: 70 g.
Example 6
The present embodiment is different from embodiment 2 in that the mixed seed ratio of the platycodon grandiflorum seed coating agent to the platycodon grandiflorum seeds is 1 mL: 90 g.
Verification example
1) Germination Box experiment
Randomly placing 50 coated platycodon grandiflorum seeds on a paper bed of a germination box, alternately performing 12-hour illumination and 12-hour dark culture every day, keeping the temperature at 20 ℃, regularly spraying a proper amount of water every day to keep the germination paper moist, counting the germination rate (the length of radicle exceeds the length of the seeds) from the fourth day of the experiment, recording the germination rate and the mildew rate when two leaves grow out, and considering that the germination is finished;
wherein the coated platycodon grandiflorum seeds are obtained by adopting the coating method of any one of embodiments 1 to 6; uncoated Platycodon grandiflorum seeds were used as controls; five replicates of each set of experiments above were performed; the results are shown in Table 1, wherein each example refers to the coated Platycodon grandiflorum seeds obtained by the coating method of the corresponding numbered example described above; fig. 2 is a diagram of a germination box for platycodon grandiflorum seeds before and after coating in example 2.
TABLE 1 germination and mold growth before and after coating of Platycodon grandiflorum seeds
Figure BDA0003052776720000041
The results in table 1 show that when the platycodon grandiflorum seed coating agent and the coating method thereof are used for coating platycodon grandiflorum seeds, the germination rate of the platycodon grandiflorum seeds is improved by 4-7% and the mildew rate is reduced by 10-13%, namely the platycodon grandiflorum seed coating agent can be matched with the coating method with a proper drug seed ratio to remarkably improve the germination rate of the platycodon grandiflorum seeds and reduce the mildew rate of the platycodon grandiflorum seeds.
2) Sandbox experiment
Randomly placing 100 coated platycodon grandiflorum seeds in a sand box, alternately performing 12-hour illumination and 12-hour dark culture every day, keeping the temperature at 20 ℃, regularly spraying a proper amount of water every day to keep the sand box moist, beginning to emerge the seeds, counting the emergence rate, considering that the germination is finished when three leaves grow out, and randomly taking out 30 plants to measure the root length;
wherein the coated platycodon grandiflorum seeds are obtained by adopting the coating method of any one of embodiments 1 to 6; uncoated Platycodon grandiflorum seeds were used as controls; five replicates of each set of experiments above were performed. The experimental results are shown in table 2, wherein each example refers to the coated platycodon grandiflorum seeds obtained by the coating method of the corresponding numbered example described above; fig. 3 is a sandbox of platycodon grandiflorum seeds before and after coating in example 2.
TABLE 2 emergence rate and root length before and after coating of Platycodon grandiflorum seeds
Figure BDA0003052776720000051
As can be seen from the results in Table 2, when the platycodon grandiflorum seed coating agent and the coating method thereof are used for coating platycodon grandiflorum seeds, the emergence rate of the platycodon grandiflorum seeds is improved by 5-8 percentage points after the platycodon grandiflorum seeds are coated, the root is increased by 0.2-0.7 cm, and the emergence rate and the root length of the platycodon grandiflorum seeds can be obviously improved.
The above is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above-mentioned embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that modifications and embellishments within the scope of the invention may be made by those skilled in the art without departing from the principle of the invention.

Claims (7)

1. The platycodon grandiflorum seed coating agent is characterized by comprising the following raw materials in percentage by mass: 2.78-4.63% of thiabendazole, 0.36-0.60% of metalaxyl-M, 0.46-0.77% of fludioxonil, 1.5% of thiamethoxam, 2.0% of agricultural emulsion 700, 0.2% of xanthan gum, 0.2% of sodium carboxymethylcellulose, 4% of ethylene glycol and the balance of purified water.
2. A coating method of a platycodon grandiflorum seed coating agent is characterized by comprising the following steps:
preparing the platycodon grandiflorum seed coating of claim 1;
and (3) uniformly stirring the platycodon root seed coating agent and the platycodon root seeds, and airing to finish the coating of the platycodon root seeds.
3. The coating process according to claim 2, characterized in that: the seed coating agent of the platycodon grandiflorum and the seed of the platycodon grandiflorum have a drug-seed ratio of 1 mL: 50-90 g.
4. The coating process according to claim 2, characterized in that: the platycodon root seed coating agent comprises the following raw materials in percentage by mass: 3.71% thiabendazole, 0.48% metalaxyl-M, 0.61% fludioxonil, 1.5% thiamethoxam, 2.0% agricultural milk 700, 0.2% xanthan gum, 0.2% sodium carboxymethylcellulose, 4% ethylene glycol, and the balance of purified water.
5. The coating method according to claim 4, wherein: the seed coating agent of the platycodon grandiflorum and the seed of the platycodon grandiflorum have a drug-seed ratio of 1 mL: 85 g.
6. The application of the platycodon grandiflorum seed coating agent of claim 1 in seedling raising of platycodon grandiflorum seeds.
7. The application of the coating method of any one of claims 2 to 5 in raising seedlings of platycodon grandiflorum seeds.
CN202110492046.XA 2021-05-06 2021-05-06 Platycodon grandiflorum seed coating agent and coating method and application thereof Pending CN113273576A (en)

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Application publication date: 20210820